LOCUS       DAYYBG010000001       115738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000001 DAYYBG010000000
VERSION     DAYYBG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 115738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 115738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..115738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.001"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..2353)
                     /locus_tag="ACHAUN_000001"
     CDS             complement(167..2353)
                     /locus_tag="ACHAUN_000001"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8458742.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2571..3254
                     /locus_tag="ACHAUN_000002"
     CDS             2571..3254
                     /locus_tag="ACHAUN_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8458743.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3335..4054)
                     /locus_tag="ACHAUN_000003"
     CDS             complement(3335..4054)
                     /locus_tag="ACHAUN_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8458744.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4366..4572)
                     /locus_tag="ACHAUN_000004"
     CDS             complement(4366..4572)
                     /locus_tag="ACHAUN_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458745.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4817..5419
                     /locus_tag="ACHAUN_000005"
     CDS             4817..5419
                     /locus_tag="ACHAUN_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8458746.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5560..6840
                     /locus_tag="ACHAUN_000006"
     CDS             5560..6840
                     /locus_tag="ACHAUN_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8458747.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6853..7581
                     /locus_tag="ACHAUN_000007"
     CDS             6853..7581
                     /locus_tag="ACHAUN_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8458748.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7650..9173)
                     /locus_tag="ACHAUN_000008"
     CDS             complement(7650..9173)
                     /locus_tag="ACHAUN_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8458749.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9425..10606
                     /locus_tag="ACHAUN_000009"
     CDS             9425..10606
                     /locus_tag="ACHAUN_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8458750.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10666..12294)
                     /gene="treC"
                     /locus_tag="ACHAUN_000010"
     CDS             complement(10666..12294)
                     /gene="treC"
                     /locus_tag="ACHAUN_000010"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8458751.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12745..15456)
                     /locus_tag="ACHAUN_000011"
     CDS             complement(12745..15456)
                     /locus_tag="ACHAUN_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8458752.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15466..16167)
                     /locus_tag="ACHAUN_000012"
     CDS             complement(15466..16167)
                     /locus_tag="ACHAUN_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8458753.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16353..16952
                     /locus_tag="ACHAUN_000013"
     CDS             16353..16952
                     /locus_tag="ACHAUN_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8458754.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17033..18046
                     /locus_tag="ACHAUN_000014"
     CDS             17033..18046
                     /locus_tag="ACHAUN_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8458755.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18154..19071
                     /gene="rbsK"
                     /locus_tag="ACHAUN_000015"
     CDS             18154..19071
                     /gene="rbsK"
                     /locus_tag="ACHAUN_000015"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8458756.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19099..19494
                     /gene="rbsD"
                     /locus_tag="ACHAUN_000016"
     CDS             19099..19494
                     /gene="rbsD"
                     /locus_tag="ACHAUN_000016"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8458757.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19494..20867
                     /locus_tag="ACHAUN_000017"
     CDS             19494..20867
                     /locus_tag="ACHAUN_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8458758.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21224..23368
                     /locus_tag="ACHAUN_000018"
     CDS             21224..23368
                     /locus_tag="ACHAUN_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8458759.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23420..24460)
                     /locus_tag="ACHAUN_000019"
     CDS             complement(23420..24460)
                     /locus_tag="ACHAUN_000019"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8458760.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24737..26539)
                     /locus_tag="ACHAUN_000020"
     CDS             complement(24737..26539)
                     /locus_tag="ACHAUN_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8458761.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26553..27305)
                     /locus_tag="ACHAUN_000021"
     CDS             complement(26553..27305)
                     /locus_tag="ACHAUN_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8458762.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27314..27676)
                     /locus_tag="ACHAUN_000022"
     CDS             complement(27314..27676)
                     /locus_tag="ACHAUN_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8458763.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27852..28724)
                     /locus_tag="ACHAUN_000023"
     CDS             complement(27852..28724)
                     /locus_tag="ACHAUN_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8458764.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28775..29263)
                     /locus_tag="ACHAUN_000024"
     CDS             complement(28775..29263)
                     /locus_tag="ACHAUN_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8458765.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29401..30192)
                     /locus_tag="ACHAUN_000025"
     CDS             complement(29401..30192)
                     /locus_tag="ACHAUN_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8458766.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30247..30855)
                     /locus_tag="ACHAUN_000026"
     CDS             complement(30247..30855)
                     /locus_tag="ACHAUN_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8458767.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30848..31165)
                     /locus_tag="ACHAUN_000027"
     CDS             complement(30848..31165)
                     /locus_tag="ACHAUN_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8458768.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31321..32049)
                     /locus_tag="ACHAUN_000028"
     CDS             complement(31321..32049)
                     /locus_tag="ACHAUN_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8458769.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32208..33089
                     /locus_tag="ACHAUN_000029"
     CDS             32208..33089
                     /locus_tag="ACHAUN_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8458770.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33326..34369)
                     /locus_tag="ACHAUN_000030"
     CDS             complement(33326..34369)
                     /locus_tag="ACHAUN_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8458771.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34372..35040)
                     /locus_tag="ACHAUN_000031"
     CDS             complement(34372..35040)
                     /locus_tag="ACHAUN_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8458772.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35182..37518
                     /locus_tag="ACHAUN_000032"
     CDS             35182..37518
                     /locus_tag="ACHAUN_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8458773.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37633..37944)
                     /locus_tag="ACHAUN_000033"
     CDS             complement(37633..37944)
                     /locus_tag="ACHAUN_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458774.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38125..39600
                     /locus_tag="ACHAUN_000034"
     CDS             38125..39600
                     /locus_tag="ACHAUN_000034"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8458775.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39647..40231
                     /locus_tag="ACHAUN_000035"
     CDS             39647..40231
                     /locus_tag="ACHAUN_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8458776.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40233..40652
                     /locus_tag="ACHAUN_000036"
     CDS             40233..40652
                     /locus_tag="ACHAUN_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8458777.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40933..41217)
                     /locus_tag="ACHAUN_000037"
     CDS             complement(40933..41217)
                     /locus_tag="ACHAUN_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8458778.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41569..42279)
                     /locus_tag="ACHAUN_000038"
     CDS             complement(41569..42279)
                     /locus_tag="ACHAUN_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8458779.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42276..43748)
                     /locus_tag="ACHAUN_000039"
     CDS             complement(42276..43748)
                     /locus_tag="ACHAUN_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8458780.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43886..44359
                     /locus_tag="ACHAUN_000040"
     CDS             43886..44359
                     /locus_tag="ACHAUN_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8458781.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44512..45063)
                     /locus_tag="ACHAUN_000041"
     CDS             complement(44512..45063)
                     /locus_tag="ACHAUN_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8458782.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45168..45698
                     /locus_tag="ACHAUN_000042"
     CDS             45168..45698
                     /locus_tag="ACHAUN_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8458783.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46181..46624)
                     /locus_tag="ACHAUN_000043"
     CDS             complement(46181..46624)
                     /locus_tag="ACHAUN_000043"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8458784.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46640..47800)
                     /locus_tag="ACHAUN_000044"
     CDS             complement(46640..47800)
                     /locus_tag="ACHAUN_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8458785.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48307..50007
                     /locus_tag="ACHAUN_000045"
     CDS             48307..50007
                     /locus_tag="ACHAUN_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458786.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50109..50942)
                     /locus_tag="ACHAUN_000046"
     CDS             complement(50109..50942)
                     /locus_tag="ACHAUN_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8458787.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51246..53411)
                     /locus_tag="ACHAUN_000047"
     CDS             complement(51246..53411)
                     /locus_tag="ACHAUN_000047"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8458788.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53671..55965
                     /locus_tag="ACHAUN_000048"
     CDS             53671..55965
                     /locus_tag="ACHAUN_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8458789.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55958..56623
                     /gene="pgmB"
                     /locus_tag="ACHAUN_000049"
     CDS             55958..56623
                     /gene="pgmB"
                     /locus_tag="ACHAUN_000049"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8458790.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56616..57623
                     /locus_tag="ACHAUN_000050"
     CDS             56616..57623
                     /locus_tag="ACHAUN_000050"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8458791.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57645..58664
                     /locus_tag="ACHAUN_000051"
     CDS             57645..58664
                     /locus_tag="ACHAUN_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8458792.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58945..60312)
                     /gene="nhaC"
                     /locus_tag="ACHAUN_000052"
     CDS             complement(58945..60312)
                     /gene="nhaC"
                     /locus_tag="ACHAUN_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8458793.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60421..61842)
                     /gene="tyrP"
                     /locus_tag="ACHAUN_000053"
     CDS             complement(60421..61842)
                     /gene="tyrP"
                     /locus_tag="ACHAUN_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8458794.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62085..63962)
                     /gene="tdc"
                     /locus_tag="ACHAUN_000054"
     CDS             complement(62085..63962)
                     /gene="tdc"
                     /locus_tag="ACHAUN_000054"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8458795.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64253..65509)
                     /gene="tyrS"
                     /locus_tag="ACHAUN_000055"
     CDS             complement(64253..65509)
                     /gene="tyrS"
                     /locus_tag="ACHAUN_000055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8458796.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65621..65826)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            66089..67390
                     /locus_tag="ACHAUN_000056"
     CDS             66089..67390
                     /locus_tag="ACHAUN_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8458797.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67459..68058)
                     /locus_tag="ACHAUN_000057"
     CDS             complement(67459..68058)
                     /locus_tag="ACHAUN_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8458798.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68312..69193
                     /locus_tag="ACHAUN_000058"
     CDS             68312..69193
                     /locus_tag="ACHAUN_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8458799.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69206..70816
                     /locus_tag="ACHAUN_000059"
     CDS             69206..70816
                     /locus_tag="ACHAUN_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8458800.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(70893..71552)
                     /locus_tag="ACHAUN_000060"
     CDS             complement(70893..71552)
                     /locus_tag="ACHAUN_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8458801.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71835..72500)
                     /locus_tag="ACHAUN_000061"
     CDS             complement(71835..72500)
                     /locus_tag="ACHAUN_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8458802.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(72647..73252)
                     /gene="dhaL"
                     /locus_tag="ACHAUN_000062"
     CDS             complement(72647..73252)
                     /gene="dhaL"
                     /locus_tag="ACHAUN_000062"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8458803.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(73280..74290)
                     /gene="dhaK"
                     /locus_tag="ACHAUN_000063"
     CDS             complement(73280..74290)
                     /gene="dhaK"
                     /locus_tag="ACHAUN_000063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8458804.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(74265..74660)
                     /gene="dhaM"
                     /locus_tag="ACHAUN_000064"
     CDS             complement(74265..74660)
                     /gene="dhaM"
                     /locus_tag="ACHAUN_000064"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8458805.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            74814..75134
                     /locus_tag="ACHAUN_000065"
     CDS             74814..75134
                     /locus_tag="ACHAUN_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8458806.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(75246..76103)
                     /locus_tag="ACHAUN_000066"
     CDS             complement(75246..76103)
                     /locus_tag="ACHAUN_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8458807.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(76211..77515)
                     /locus_tag="ACHAUN_000067"
     CDS             complement(76211..77515)
                     /locus_tag="ACHAUN_000067"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8458808.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(77512..78021)
                     /locus_tag="ACHAUN_000068"
     CDS             complement(77512..78021)
                     /locus_tag="ACHAUN_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458809.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(78196..80331)
                     /locus_tag="ACHAUN_000069"
     CDS             complement(78196..80331)
                     /locus_tag="ACHAUN_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8458810.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(80414..82069)
                     /locus_tag="ACHAUN_000070"
     CDS             complement(80414..82069)
                     /locus_tag="ACHAUN_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8458811.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(82071..82385)
                     /locus_tag="ACHAUN_000071"
     CDS             complement(82071..82385)
                     /locus_tag="ACHAUN_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8458812.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            82555..82815
                     /locus_tag="ACHAUN_000072"
     CDS             82555..82815
                     /locus_tag="ACHAUN_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8458813.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            82829..83143
                     /locus_tag="ACHAUN_000073"
     CDS             82829..83143
                     /locus_tag="ACHAUN_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8458814.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(83873..84427)
                     /locus_tag="ACHAUN_000074"
     CDS             complement(83873..84427)
                     /locus_tag="ACHAUN_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8458815.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(84553..86482)
                     /locus_tag="ACHAUN_000075"
                     /pseudo
     CDS             complement(84553..86482)
                     /locus_tag="ACHAUN_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(86788..87504)
                     /locus_tag="ACHAUN_000076"
     CDS             complement(86788..87504)
                     /locus_tag="ACHAUN_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8458816.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(87505..89328)
                     /gene="glpO"
                     /locus_tag="ACHAUN_000077"
     CDS             complement(87505..89328)
                     /gene="glpO"
                     /locus_tag="ACHAUN_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8458817.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(89342..90838)
                     /gene="glpK"
                     /locus_tag="ACHAUN_000078"
     CDS             complement(89342..90838)
                     /gene="glpK"
                     /locus_tag="ACHAUN_000078"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8458818.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(90974..92434)
                     /locus_tag="ACHAUN_000079"
     CDS             complement(90974..92434)
                     /locus_tag="ACHAUN_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8458819.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(92424..93719)
                     /locus_tag="ACHAUN_000080"
     CDS             complement(92424..93719)
                     /locus_tag="ACHAUN_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8458820.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(94051..94728)
                     /locus_tag="ACHAUN_000081"
     CDS             complement(94051..94728)
                     /locus_tag="ACHAUN_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8458821.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(94758..95849)
                     /locus_tag="ACHAUN_000082"
     CDS             complement(94758..95849)
                     /locus_tag="ACHAUN_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8458822.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(95950..96384)
                     /locus_tag="ACHAUN_000083"
     CDS             complement(95950..96384)
                     /locus_tag="ACHAUN_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8458823.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(96968..98425)
                     /locus_tag="ACHAUN_000084"
     CDS             complement(96968..98425)
                     /locus_tag="ACHAUN_000084"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8458824.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(98422..100317)
                     /locus_tag="ACHAUN_000085"
     CDS             complement(98422..100317)
                     /locus_tag="ACHAUN_000085"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8458825.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(100375..102150)
                     /locus_tag="ACHAUN_000086"
     CDS             complement(100375..102150)
                     /locus_tag="ACHAUN_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8458826.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(102200..103696)
                     /locus_tag="ACHAUN_000087"
     CDS             complement(102200..103696)
                     /locus_tag="ACHAUN_000087"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8458827.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(103890..105512)
                     /locus_tag="ACHAUN_000088"
     CDS             complement(103890..105512)
                     /locus_tag="ACHAUN_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8458828.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(105840..107153)
                     /locus_tag="ACHAUN_000089"
     CDS             complement(105840..107153)
                     /locus_tag="ACHAUN_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8458829.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(107398..107622)
                     /locus_tag="ACHAUN_000090"
     CDS             complement(107398..107622)
                     /locus_tag="ACHAUN_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458830.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(107642..108064)
                     /locus_tag="ACHAUN_000091"
     CDS             complement(107642..108064)
                     /locus_tag="ACHAUN_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8458831.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(108206..108916)
                     /locus_tag="ACHAUN_000092"
     CDS             complement(108206..108916)
                     /locus_tag="ACHAUN_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8458832.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(108934..109431)
                     /locus_tag="ACHAUN_000093"
     CDS             complement(108934..109431)
                     /locus_tag="ACHAUN_000093"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8458833.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(109468..109920)
                     /locus_tag="ACHAUN_000094"
     CDS             complement(109468..109920)
                     /locus_tag="ACHAUN_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8458834.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(110055..110660)
                     /locus_tag="ACHAUN_000095"
     CDS             complement(110055..110660)
                     /locus_tag="ACHAUN_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8458835.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(111110..111730)
                     /locus_tag="ACHAUN_000096"
     CDS             complement(111110..111730)
                     /locus_tag="ACHAUN_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8458836.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(111732..112601)
                     /locus_tag="ACHAUN_000097"
     CDS             complement(111732..112601)
                     /locus_tag="ACHAUN_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8458837.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(112624..113031)
                     /locus_tag="ACHAUN_000098"
     CDS             complement(112624..113031)
                     /locus_tag="ACHAUN_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8458838.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(113050..114339)
                     /locus_tag="ACHAUN_000099"
     CDS             complement(113050..114339)
                     /locus_tag="ACHAUN_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8458839.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            114397..115736
                     /locus_tag="ACHAUN_000100"
     CDS             join(114397..114838,114838..115736)
                     /locus_tag="ACHAUN_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8458840.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT        34003 a        23848 c        20273 g        37614 t
ORIGIN      
        1 gaggtgtgac aaaattcttg tcacacctcc cttttccgaa taaactgtgt tccagaaata
       61 gcttcttcga aaataagctg aaatttcaca aaaatttgag aagcaatttt cgtacattcc
      121 ttcttctttc ttgaaactga ccacttctgt cacaccctct cttctatcac tttccttttt
      181 tcacacgact cccggtttct ttgataaatc tcgatacttt caattttctt gaatttttca
      241 tttgaggtac acatagatac agacgatttc ttgctctagt catcgcaaca taaaatagtc
      301 ttcgttcttc ttctatcgtc gttactggtt ctgcttttat ttcattaaag tcgattactt
      361 tcttttctgt cgctccgtta taaggcaaac ttccttcgtt acaaccaatc aaaaagacat
      421 ttcggtaact aagaccttta cttttgtgaa tgctctggat acatagtgca tcaagttttc
      481 cttgtctgta agcaatcaag cgggttttta attctttagc tgtcgcttgg gtttcttgcg
      541 ataaggcttg gagtcttctg aaagtagcat gctgctttac ttcttctcgt atccattctt
      601 gatcactctg gtcgatttcc ttagctatac gtggcagatg aagatagctt tgaacaagcg
      661 aatagatttc ttgatgctgg tctgttttac gcaacgcttc ctcaaactct cggatttctg
      721 cagatttcga ttttaaattg gggcttatcg acaaaatata atctgcaacg gctatatttt
      781 gatatttagg agaaaagtat tgatcgcatt cacttttttt caggtagcag tctgccatca
      841 gctgccggag caattgctgg taggcctgat ttttcttttg cagattcagt gctgtttgcc
      901 aatgaacaat tttttgatag aaatgttgga aagacttgat accaaagata ccttcatatg
      961 gactatctgc ttccagtaca aaaggaatat tcgttttcag cagagattgg acaagctgct
     1021 gcgtttgtga agctgatcgg tataatatga ttgattcctt atatttccca tttttttgaa
     1081 taaatgtctg aatttgtttg attagccact gactttctgc tttagtatca gaaaacaaat
     1141 aggcttccat aacacctgct tctttttgtg ctggaatcat cgcctttggt atccgatcat
     1201 ttttattata gctgatcaat ctatttgctc cttggacaat agctgcccga gaacgataat
     1261 tagttaataa gaaaagagtt ttggtaggat attctttttc aaagttcttg atgtatgctg
     1321 gctctgatcc tctgaatcca tagatactct gatcatcatc tccaacaaca atcaacttga
     1381 tcctgttttc ctttgtcatc cgtttcacga tttcccattg aagtggatta gtatcttgaa
     1441 attcatcaat gaatagataa tcatattttt ggccgatata atgctggatc tcctcattct
     1501 ccaaagcgct tttcagattt tggagtacat cattgaactc tagcaattgc tggcttcttt
     1561 tttgttcttg gagttccgtg agcaatccat acagtcgttc tgatatcggc tgtcgtgcat
     1621 tcttatcaat ggaatcatat ttttctttaa ctaactgaac catttcttct actgaaaggt
     1681 tcatattctg ccaatagtct aaccgagaag caatcgtttt ttctccatat tgatcaaata
     1741 tataaagatt atatttacgt atcagctgtt gcaaaacagc attcactttt tgtgcagtat
     1801 cctttgaaaa gtctccttgt attccttgca ttgctacttc aggaaatttt cgacaaattt
     1861 cattgtatat cctaaaaaag atactatgga atgttccgat atggacagcg gtttttctct
     1921 ggctgaaatt atctactcgt ttttttagtt catttgcagc gttccttgta aaagtgatca
     1981 ttagtatgcg ttcttcgtct acttgttttt gcgtcatcag ataaacagct tttgcacaga
     2041 ttgtttgcgt tttgccgctc ccagctcctg ctatgattcg aagatgtttc ttcatatcat
     2101 atgtgatagc tgctttttgt tcttgattga agcgattata ggaacgtttc tccatcattt
     2161 taaagaaaga gctgtttcct tcactttctt ttacttgtac ttcttcctca ataagtccgg
     2221 gtgcttcgcc ataagccgct tcataggtag caatgatttc ttcttgtagc cgaaagactt
     2281 gttgaagctt tttatacttg ttctcccagc gtttcaattc tttttgtaat cggagaattt
     2341 ctttttcatt caactgattg atttccttcc catctttctt ctttgaattt gtttatttca
     2401 agttggttat ttaaagtttc gttgtctatt ttttctttat actaacagac ctttctaaaa
     2461 atgaaaatgg gaagcgatat gattttagtt ttctgaaaat ttttcctttt tttcaatcgt
     2521 ttatgctaag ataaggaaga caaaaatatt cataaaaggt gatgaaaaaa atgcagactt
     2581 cctttcacga ggggcgtaca ataggacttt tgctgacatt tatcggagga gccatggaca
     2641 gttatactta tatccaatat ggtgcttttg cctctgctca aactggcaat atcattctat
     2701 cgatcatcca agcatttgac ggagaatggc taagtgttgg taaaaaagta ttgtctaccc
     2761 ttttcttttt cttagggatt cttctaacaa agtatctaat tgattatttt agaaaaaaag
     2821 aaaaacattt ctggcgcttg tttgttctat attatgaagc agtgattttt ttctttgtta
     2881 gtttgacacc cgtccatgag catcccgcat tagtaacgat tttgattgct tttactgcat
     2941 cgatccagtg ggtagctttt gataaaatca acgggcgtgc ctacactaat ttatttacaa
     3001 ctggaaattt aaaaggggta gcaacgaact tatatgatta ttgtatttct aaagaaaaag
     3061 cagattttga tcgatttttc cactatctaa gtgtcgttct tgcctttatc gcaggagcta
     3121 ttgtccttgt tttctgtcat catctgtttg gcgcccattc gattttgatc gtttccattt
     3181 tatttttgat catggccctc actcagacat ttcatttatg gcaattttat catacgaatc
     3241 ggttcactat ttgatctcaa tgaatcaaaa aaactaagca agatgctgct cacagagaaa
     3301 tatctgcgag tgagcaattt gcttagtttt tatattattt agttgtaaag attccctttg
     3361 ctttcgggtt atttgtagtg tagcggatcg actgggcatc tgcctcttct gtcattttct
     3421 ttgcccgcat cttgatttct tgtacgattt gttctccaca tgccggatcg gctacgaaat
     3481 catatttgtc cccatcgatc actattttgg gacatatatc aaactgttca taccactctt
     3541 cgtatcgtcg attgagttct ttatagtagg aatagagttc aggatcttgt tctagctgtt
     3601 catattcccg gcctcttttt ttgattctcg ctaacatagt ttcaaaggat acacgaatat
     3661 aaaccagtag atctggtttc ttttgtggcg aaatttcttc taactccttc agcatcgttt
     3721 ctaaaagacc ttcatactgc tttacttcta cttcagaaac acgccctaaa tctgcattca
     3781 aatgaaacag catactatct tcgtagatgg agcggtctag tacgttgtca tcatggctga
     3841 aagcatcttt gattgccaaa aagcgtttgt tcaaaaagaa aatctgtaat aaaaaagtat
     3901 acttttctgg attagaataa aatagcggca atacttcatt atcttccacg ttctcataaa
     3961 aaggttttgt ttctaaatct tgggcaagca tttcagttag tgtgcttttc cctgcaccga
     4021 tcgttcctgc aacaatgatc acaattatcg gcatccttcc tgtaataaaa tctgttcttc
     4081 attttactac ttcatttata gaaataaaag ccttgacttt atctgctcct taaaagctac
     4141 ttctctaaaa gtagccaaaa gtaactccta ttcatgaaaa taaaggctgt gacaaaagtc
     4201 ttgtcgcagc ccttttttca aataaacagt attctcctgc caacacctcg gtcacaaggc
     4261 aatattcata aaacatgaaa agctgttttt taacattatt ccttgtaact cagagctaaa
     4321 cgggagactc acaacctctt tatgctctca acattttatc tgtttttatt cttctattgt
     4381 acttgatttg cgtacaacta actgccagtg cagattatga gcggtatcat tgtcttctac
     4441 tgcgcatttc atgacacgta tcatcacact attttcatag attttttcta tttttccttt
     4501 tattggtgat tcaaataaat cagatttaaa tgcatatgtt tcgccgactt caaacaattg
     4561 ttcattttcc ataagaatcc ctccatcacc ttttataagg tcagtaattt tttaacataa
     4621 atcactcgaa aaagcacaaa cttgtcataa aaagtacttt tcgtatcaaa actgtcactt
     4681 tttttggtaa ggttcttgga aaacgaagat ttttttagga gatcatgacg gttttgatgt
     4741 caaaaccact ttttatgaaa caaaagacgt tttttatttt ttttagcata aaatttgtgg
     4801 tggaggtacg tatgagatga aagataaagt tgtgaacaaa ttattagaag ccttattcag
     4861 cggagacaaa tcggaagaag atatggtgta tgtccaagta aaatttgtct tagaagtttt
     4921 attgaataat cttggaaaac tagcggtcgt tctagtcttt tctctggtga caggctcatg
     4981 ggcggaaaca ggaatcacct ttctttctta tatctgcatc cgcagatatg cttatggatt
     5041 gcattccgac tctgaattcg tttgtctgct gtggacacta ctttatttat ggggcgttcc
     5101 cttggtgatg aaacatctgc aattgactat ctccttccca atgatggtct ttttgttaat
     5161 cagctgcttt ttgttactgc ttcgctatgg atcaagaggt acagcaatca atcccattga
     5221 acccgagaaa cgtcccccat tattaaagaa ggcgatcagc atgtttctaa ttttttctct
     5281 cattacgtta tttttctcag cttcttactt ttctacctat ttgctacttg ggattgtttt
     5341 agaaatagct acgttgcttc ctattacaaa ctacttgatg aattttggag gaatctttca
     5401 tgaaaaaaac aatcattaaa caattaacta acttatctga cttttttgta tctggctgct
     5461 gctttggtat gagttctgaa ttcaaaatcc ctgaaaaatt aaaaaaataa tacgccaatt
     5521 gtgctataat ttggtaaaaa tcaatgggag ctgtacttta tgtttttatg gtccatagcg
     5581 ttgcaatatt tgatttacac aatttgtatc ttattgattg accatcacat ccgatacaaa
     5641 tatctgtttg cgggagtcat tctgatttct tgtattttga ttggatgggt ctattcgatt
     5701 ttagggattt ttactgctct aattgctata ggaatgctga tgcttacttt tttcttcttt
     5761 tcgacttcgc gtcaacaatt ggtttacgcg cttccttttg ggttattgac gtcgctttta
     5821 ggagatcact taacaagcat cggcgatttt tttgttttga aatcttcggt tattgaacca
     5881 ggagattcat tgcagtattt tcacattgtc ttatccgctt ttttatctgc tttattagct
     5941 ttttgtttaa aaaaatcgtt agagacttgg tttcacactg tggaccgccg tatgatcggt
     6001 acgatcggga cattgatatt atttacatac tatatcatta ttttttatac ccgtttttct
     6061 ggtgaaacac ctgatgtctt agctttgaat acgagttttt tcattctata tctgtgtgtt
     6121 ggcctgatca tcttggttta ttattggaaa atctctaata agaaattagc tgatcagtta
     6181 ttagagcaac aagtcaggat gcaacaggat tatatcaaag atctagaaaa aaactatcaa
     6241 gacttgagag aattcaaaca tgattaccaa aatctgcttt tttctcttca cagctatatt
     6301 tcagaaggcg acttagaagg attacggtct tattatgatc aaacgatatt acctacacgc
     6361 cagatgatga acctttttcc ggccaatcta agtatgttgg ataatatgaa agttcctgag
     6421 atccgtagtt tactgtcatt aaaactcatg atggcacagg aaaaagggtt gatcgtccag
     6481 cttcattttc ctgagccaat cgaactggat acgaaacata cggtaaacct tgtacgtatg
     6541 cttggtatta tcttggacaa tgcgattgaa ggagctagtg ctgcaaagaa gaaaaaaatc
     6601 gaattgacca tactcaagaa gaatcattcc gtgatgattc gtgtgatcaa tacaacaaca
     6661 aatacattac cgttaaacca gttgaaacaa aaagggttct caacaaaaac aaatccaaaa
     6721 aacgaaggcc ttggcttgtt tatcttggat gaattgacaa agaccaatcc ttatcttttc
     6781 cttgaaacat caattgaaaa ccagcgtttt tctcaaacga tttatataca aataaaataa
     6841 aggagtaatg atatgattcc catttatatt tgtgaagacg atccaaaaca attagaagtt
     6901 ttatcaacgg tgatcaaaaa tagaatcatg attgaaaact tagacagtta tgtccatatt
     6961 tctacctctg atccggaaga acttctcaat gcagccaaga tcagaacttc ttcttttggt
     7021 atttactttt tagatatcga actagaagac agtgaaatgg atggtgtagc gatcggtaaa
     7081 ttgatccgtg aattagatcc tttaggaaaa ataatctatg tgacttccca tacggaaatg
     7141 tcgatgaaaa tcttaaaaag caacatcgaa ccgactgatt atatcgtcaa agaagatctt
     7201 tatgatttaa aagaaaatgt cgagcatatc ctgagcaaaa ttttcgaaga ttttcaaatg
     7261 tcccaaatcc aaaaagatat cttcaaaata gagtttaatg atgagatcaa atttttaccg
     7321 atcaaagaca tccaatattt ttctacttcg ctaggagcgc cacataaact ggaagtccat
     7381 ttgactcatg cccaattaca attctatgag aagatcaaag aaatcgagaa aatgcatcgg
     7441 aattttatcc gttgtcataa atcctatgtt atcaatacac aaaatgtgcg gtcgattaat
     7501 aaaaaaacca gagaagtcac gttggcaaac ggcgagacca tcccagcctc tatccgtgga
     7561 ttaaagaaat tgatttcata aaaagaaccg aatagtttgg accgactaaa tgaccagccg
     7621 atccaaaccc ttcggttctt ttttgcctat cagtgcaaaa gcatttcctg tgcaatttca
     7681 cgtccatcca ttgtatctgg ataaaatgtt ggccaattct ccatttcttt cagcaatact
     7741 tcttgacttt catttcctgc atataaatga aaatgttcgg agtcagccgg tgcaatcgtg
     7801 tgatcactga attgaatata tctaaaagcg ccactttcct tatcttctgc ttcgaataga
     7861 taccttacgc cacggtttcc tgcctcgtag gtcaagattt ctttccctac ataacgataa
     7921 tttgaacgtt tcgttgttcc attcttaaac gtaaatgcca tctctttctc actgatcttg
     7981 attgaagcaa tatctgtttg atagcccttc gtataatatt ctttatattc ttgtgctgtc
     8041 ttatcctgat tcaatttttc tttatattca aaaacaggat caaaagtccc atcttctaca
     8101 tacgtatcta ctgtttgcca agttcctgcc caatctgaaa gagagcgatc ttgtacatcc
     8161 tcgtcttcaa aataaccgtt gtagacgctc ttttcttttt tgggggtcaa agtatcttct
     8221 agcgggtttt ctgtctctgt cgtttttttc aacgcttcga ggtttgcttc catgatagaa
     8281 agatagtttt ctccattttc tatttgttca ttcgtcaatc cttccaaagg attcaatact
     8341 tctaaagata cacctgcttc gtttgctaac gttctggcaa tactatcttt ggcgttttct
     8401 tcaaaataaa tatagttgat tccatgttct ttaacaaagt gtttcagttc agcaattctc
     8461 gccgcagacg gttcttcact agaagaaaga ccagcaatcg atacttgatt caatccatat
     8521 tctaacgcta gatagttgaa tgcagcatgt tgcgtgacaa aagatctttg ttttgcttgc
     8581 ttaaaggttt cttgataaag ctgatggagt gcttgaagtt tctcggtata ggctgctgca
     8641 ttttttgtcc atacttcctg tttttctggg taagcttcaa tcagctgatc acgtatgttt
     8701 gctacctgct tgattgctaa acttggagct agccatacat gtgggtctag ctcatgtgta
     8761 tggccttctt ccccgtgttc atggtcatga tcatgtcctt cttcacttcc cggaagtaaa
     8821 agcatacctt ctgtcgcttt tatcgtatgg acattgcctt cttgtaatgt tttttggatt
     8881 gcggggaccc atgtttccat gttttcatca ttataaacaa agatatctgt atcttggatc
     8941 tttgccatgt ctttagcaga aggttcataa tcgtgggaat cagtccctgc tggaatcaat
     9001 aactctactt ctccttcatc cccgacgatt gcttttgtaa aatcatacat gggataaaag
     9061 gtcgtcatca cgctcaattt ttctttactg tcctcggctt tcccacttgc cccgcaagca
     9121 gttaacatac ctgctaaaac cagcatggcc aatataccta tcaatcgttt catttatgac
     9181 actcctcttc tcatcttctt tcatttgaat gttccgattc gcttttcgca aaccgtaata
     9241 gttacgtttt agcaacagga tttaatttat catatggtta aaagaactgt caagcgtttt
     9301 cttattttaa ttgatgagaa agtttatatg aatgattgac gatatttttg caatcaacct
     9361 ctatcatttt ccttttatac ccgcttttgt ctataatgaa tttatagata aaggaggaaa
     9421 aagagtgaaa aaagaaacag atacaacgaa tcgtttgatc cgctttttag gaggaaaaac
     9481 ctcttactat gttttaggaa tagttatctt acttgcgctg atcatttatt ttttccatca
     9541 gatctcgttt atattcaagc ctgtcggaat cattatcgct actatcttgc ccccattatt
     9601 gtttgcactg atcttgtatt atcttttgga accagtcatc aaacgtttga gcaagcacct
     9661 ttcgaggaac ttgtcagtca ttatcgttta tgtgattatt ttatttttat tggggcttgg
     9721 aagcatatgg cttattcctt ttcttgaaga acaggcaaag acattgatca gtagtttgcc
     9781 agatttgttt caagattttc aagagagtct ccgacaattt ttggagaaaa caccttttgc
     9841 cgattcattt aatcaatttt tcgcttctat tgacgacgtg acgtctgata tagctggatt
     9901 tattggaaat tattgggaaa gtggcgcaca acggatcagc aacatttttt caactgttac
     9961 agctattttt attactttat ttactggtcc aattgtcgct tttttccttt tgagagatcc
    10021 tcaaaaattt tatcagtcag ttttagcgat tgtcccacca gcatttcgta cagattttaa
    10081 aaatttgaca aagattgcta atcaacaatt gggttcattt ctaaaggggc aaatcattgc
    10141 ttcatttatc cttggagcag tctactgggt ttgtttctta ctgatcggat tagaatttgc
    10201 tagtgtttta gctatttctg ctgggttgtt atgtatcatt ccatatattg ggccgtttat
    10261 cgccttcttt ccaggtttga tcattgcatt ccaagactct acttttatgg ctgtcaaatt
    10321 tgtcattgtc tggtttgccg tacaacttct ccacggtgac ttggtgattc ctcgagtaat
    10381 gggtgatcgt ttacagatcc atccgattac gatcttaatc gttttacttg tcatgggtga
    10441 tctaatggga attgtgggcg ttatttttgg tattcctatc tatacgttag tcaaattact
    10501 cgttactttt attttccgta aattcaaaca acgctacaat aaattttatg gcgataaagg
    10561 agagtatgaa aactcttcat tttctgaaga agattatttt aaataaaaca caaaacgtcg
    10621 atcttacgag aatagctcgt aggaacgacg ttttgtgttt ttgttttaat ttttcagcaa
    10681 agcaattgcc tggtatggtt ttagctccag actttgatga agaacagtat cggaataatt
    10741 atcgataaga atattggctt ctgtgaatcc ttctgggatg gcgatccttg tatctttatc
    10801 atagaaattt gttaatacaa gcagctgctg tccttcaaat tggcgaagaa agccataaac
    10861 ttccggatga tttgcttcat aagcatgata atctccttgt gcaacgattg gatatgtttt
    10921 tctcagccga atcaatttct tgtaatagga caaaatagaa tcttcagaag atagttcttt
    10981 ttctacatta atctcttcta gcttgcctcc aacttttaac caaggtttcc ctgtagtaaa
    11041 tcctgcttgt tctgtgtctg accattgcat gggtgttcga gagttatcgc gagattttgc
    11101 tttgatccga cgaaacgctt ctttttctgt gaatccttgt tcaaggagta acttataagc
    11161 attcaaactt tctacgtcaa tatagtcgga aatcgatgaa tagtctgggt cgatcatgcc
    11221 gatttcttct cccatataaa tatagggcgt cccacggttc aaatggatca tggttgctag
    11281 catctctgct ccttgttttc gatactgatc gatagctaca aatcgattga gcgcacgtgg
    11341 ctgatcatga ttattaaaaa atagagcatt ccagccgtta ccctcactca tcttttctcc
    11401 ccatgtatga aatagtcgct ttaattctgc aaaatcaaat ggaggagtag accatttatt
    11461 cccattttca taatcgactt tcaaatgatg gaaattaaag accattgata attcttctct
    11521 actaggttct gtatacaaga tacaattttc gatcgtcgta gagctcattt caccgactgt
    11581 gatgatagtg gaatccgtcc caaacgttgc ttggttcaat tcttttagat acgtatgggt
    11641 gattggtttg tccgtatatt caggtttgcc atcattttct ttattatttt tcaattgttc
    11701 gtctttaccg ataacattga taacatcgaa acggaatcct tttacccctt tttccatcca
    11761 aaaacgaaca actttgaata gttcctctct tactgcttgg ttccgccagt tcaagtcagc
    11821 ttgtgtccga tcatataagt gcaaataata tttccccgta tctccaaatg gcgaccaagc
    11881 acttccacca aattttgata cccaatccgt tggctcttct cttaaaatga agaagtcctg
    11941 ataatgctgg tcccctgcta gtgctttttt gaaccattca tgttctgtcg aaacgtgatt
    12001 cagcaccata tcgagcatga tttctatccg gtatttcttt gcttcggcta ttagctgttc
    12061 gaattcctcc atcgtaccaa agataggatc gatggctgtg taatcagaga tatcataacc
    12121 gttgtctttt tgagggcttg gataaatagg gtttagccaa atcatgtcga tgcctagatc
    12181 attcagatac gggagttttt ctttgattcc ttttaaatct ccaattccat caccattact
    12241 gtctttgtaa gatttgggat agatttggta aatgactttg tctttaaatg tcatggatat
    12301 ttacctccat tcaactgatt tattacctac cacaacttat atatacaagt tatcatttat
    12361 tattataact gaaccgaaag cgtttgtaaa atttatcttt atacattcta agaggtctgt
    12421 tgcccaataa aaaacaagag atcagacatg tatttgtcta tcctcttgtt taaaatagca
    12481 aaatattctt gtttcttcta taaatggtgt tcaaaagctt ttatctacgg caatcagtct
    12541 tctcaaatgt aaaacatgag gagctaattt tatttgagta ttctttttgc tcgatgcagg
    12601 acggcttttt tacaacctct gattcatggt gttcaatcag ctgtccttcg gtattagctc
    12661 aaaaaacggc aaaatatgaa aaactatttt gccgtttgtt tctctaatac tcaggtcaaa
    12721 ccgctgattt cacaacctct gtttttattc attcgatttc aacgcatcaa tcatatcgat
    12781 atgctttaat ttgaaatggg taacaatcat cacaataacc gtaaatacga tagttaatcc
    12841 tgctgataag acataagccc accatgtgat cactaatgga aagatgacat tctccataga
    12901 cgcttgttgc aagatgaagt cagtcaatat atatcctaca ccaaatcctc cgatgattcc
    12961 taaaagagta aagatgatat tttctcgaac aatgtacatc gtgacttctt tatcaaaaaa
    13021 tccaaggact tttattgtag ataattcgcg tacacgctcg gaaatattga tattagttag
    13081 attatacaag acgataaaag ccagcagtcc tgataatacg acaaagatta agacaaccga
    13141 gtctaagttc gccattgatt cttcttgcgt ttcgatttgt ttggacatga aagacgtatt
    13201 tgtcacagcg tctgtggcaa gcagtttctc ggaaagcatt tcttcttctt ttgttgtcat
    13261 cttatctgta cgcagcaaaa atgcattatc catcatcgat tgatcagtaa ctgaagcata
    13321 atattcaggg gacatataaa taaagctacc tagatagttt ttcacgattt cttttacttc
    13381 gactttccat tctttttgat cttcatcata aagcgaaata tgatctcctg tagataagtc
    13441 aaatagttct gctacttttt gcgtaatagc gattcctgtt ttaggcaagg agcgtgggtt
    13501 ctcttcaaga tcagttaaat ggataaaacg atccaaatca gctgttttac ttggtacgat
    13561 cactgtcaga ctttgcgaag catgtccttt ttttctcaat tctacggttt gagtatgaat
    13621 agctaggatc tcatttactt tttcttgctt ctgcagcata tccgctgctt tttgacgact
    13681 atcttccgtg gatgaatcca atgtaacgat tgcttgatag tcaatgatcg gtccaaattg
    13741 tttggctgaa acagaactta gagaatcttt caatcccacg cctgcaacca tcagcccggc
    13801 acatccagca attccgataa tcgccataag cattcttgac ttatatcgga agagatttcg
    13861 gtaactgatt ttttgattaa agctaaggcg tgaccacagc ggagtgatgt attcaagaaa
    13921 aatgcgtttc cctggcttcg gtgctttcgg ttgaagcaat gcagcaggcc gttctcttaa
    13981 atctttgaat aaaacgatca ttgcagcgcc aaatgcggca atgaagaatg cggcagctgc
    14041 ttgtaaaatc ggcggccagt cataaaaaat aagcgcactc ccaatatcat accgctcgtt
    14101 agacagctca aaaatgatcc ttggcaaaag attcgttcct aatatcgttc ctaaaacaat
    14161 acctatccct gaggctagac ttgcatagat tgcatatttt tttgcgattt caactttcgt
    14221 ataacctaac gcctttaatg tcccgatttc cctgcggttt tcttctacca tacgtgtcat
    14281 tgtcgtaaag gtgatcaatg cggcaataaa gaaaaagaaa acaggaaaaa cattggcaat
    14341 cgcagcgata cgatcagaca gatcaccaaa ttcttgaaaa ccgacatttt ctgaacgttc
    14401 attgtatagg tatgatggtt tagttaaatt ttcaattttt tctgcatttc gattcaattc
    14461 ttcctctgca gaggccagtt cacgttcttt ctcaactaat tgcctttctt gttcagcaag
    14521 ttgagcttct tgttgagata atgtttgaac agcgattgtt cgttgcgctt caggaagctg
    14581 ctggatagtt gctgactgtt gcgctaactg ttttttagca tcttctaacc gtgttcttgc
    14641 ttgcgttgtt tgtgctttgc catccgctat tttttgctgt gccggttcta attcttcgtt
    14701 ggccgatttt ttcagctctt ctaagcgttc ttcgggtcga tcttgtaaat acttttctgc
    14761 cttttcttga ttttgctcca ttttatcttt gtaagctttg ctgtacgtat tcaaattctt
    14821 tacgtttgta aaatcaagat ataagacaga ataaatatct gttttaaaaa cggattcagg
    14881 taaatagaca aaataatcga cgctaccatt tccgacgttc gcgtatcctc tctccaaatt
    14941 gttgatgaac aatggagaac ggacaaagcc cgtgatggta tactcttttt ctttcaattc
    15001 atctgaatca atctgataaa tatcaccgat tttataccct tcatcttccg ctttttcatc
    15061 caatacaagc tggtttggtt tttccgggag ttttccatca gttaattgta gcttgttctg
    15121 cttgttattt ttgttataag aaaatatttg aatcacttcg tttttgccag tattggaata
    15181 atagatctgt tttcctgttt ctacttttgc atctgggatc ttttctgctt gagcaatatc
    15241 tttttgtgtc aagccaccag tagaaacgat ttgcaaatca gaaagtcctg ctgtaccaac
    15301 atagtgatcc atcgtttttt gcatgacagg tccagcagtt ttgacaccta caaataaaag
    15361 tgttcctagc atgacaataa ggatgattgc aatgaatcgt cctttagatt gccgaatttc
    15421 gcgaaaacct gctttaagat atgttcttgt tttcataaaa tccccctacc atacaatctc
    15481 atcaatggat gaaggatgat catttaattc aacagacctt actttagcat cattaatgtg
    15541 aatgactcta tcagcgatgg gcgctaaagc agaattatgt gtaatgatca aaacggtatt
    15601 gccgtgttgt ctgctagcgt tttgcaataa tttcaatact tgcttgcccg tttcaaaatc
    15661 cagcgcgcct gtcggttcat cacataaaag aagtttggga tttttagcta gtgcacgagc
    15721 aattgaaacc ctctgctgtt cgcctcctga taattgggac ggaaagttat tcaatcgatg
    15781 agctaggccc acttgcctca atacctctac aggatcaagc gcatcaggag aaacttccgt
    15841 tgccaattcc acattttctt ttgctgttag attaggaacg agattgtaga attggaaaac
    15901 gaaccctaca tctgttcgtc ggtaagcagt aagctgttta tctgaaaatt gcgcaatatc
    15961 tgtgtcatca ataatgatct gaccttcatc aggtgtatcc atcccaccta aaatattaag
    16021 aatcgttgac tttcctgctc cacttggacc aagtataatc actaattcgc ctttttcaat
    16081 tgaaaaagaa atatcgtcat ttgctgtaat ggtcgtttct cccattttgt atcgtttgta
    16141 ttcatttttt acttcgacat acttcactct gattcctcca ttttcacgat atttctcgct
    16201 atatctatgc atcgaatgcg ttttctcaca cttatttatt ttaccactga atttctctag
    16261 actaaagaaa ggaatccttt aatttagttg acaaataaac gatttatgat ttacttacat
    16321 aaaaaacatt gttatttgaa agaaggaaca tcatgaatga tttaaaggaa aatatgattt
    16381 ttgatttatc aaaaactgct tttgtcgcga tcgacatgca aaaagagatc gtaaataacg
    16441 gtacactctc tccacacact gcagcatcta ttctgacaaa caatgatttg ttagtaagaa
    16501 cattgaaacg taccgctgcc ttgaaagttt tagtgaacgt agacatctcc actttccctt
    16561 atttgagtca acaaactgat atgggtggaa tgactgcttc tgtacctcca gaattcactg
    16621 atttactttt gaaggattct ttgaaagata cagataatat gttgactatc acaaaataca
    16681 atccaagtgc tttttttggt acttctcttg atcttcaact tcgccggcgt ggaatcgaaa
    16741 ctatcatttt aagcggtgta gcgacaacaa acggtgtata tgctactgca ttagatgcct
    16801 ttcaacacgg gtaccatatc gtgcttgctg aagatgcttg cagtgatcgt gacaaagaat
    16861 cccatcaatt gttcataaaa aagatatttc cgaaaactgc acgagtacgc tcaacgaaac
    16921 aaataatcga agctatccaa caaagcaaat aagtactggc acacgtgatt ttcatttact
    16981 tctttttgat ttcatgggaa aataaccaca ggatttagat aggagtatga acatggtgaa
    17041 aaaaaagatc acaatcaaag aagtcgctaa actttccggc gtttcaatca caactgtctc
    17101 cttgatatta aatggaaata cggagaaatt cagtccgaaa actgttcaga aagttttagc
    17161 tgccaaagaa gaattaggct atcagcctga ctattttgct agacaaatga ttaccaagga
    17221 aacgaaaaca attggtgtac tcgttcctga tatcacgaat ccattcttca gtacactgat
    17281 gcgaggaatt gaggatatct tgtataagca gaatttcgtc acgatattat gtaatgctga
    17341 ttctgatcat caaaaagaaa tcgaatatct agccgagctc acacgtcgag gtgtggatgg
    17401 attcattatc gcgacttctg ctgtctctac agatgcaatc aacgaaaatt tgaaaaaaca
    17461 aggccggccc ttcattgttt tggatcagaa aaaatctgaa ggatttagtg atgccgtacg
    17521 aacagacgat tttcgcggtg gttacctagc gggcatgcac ttgcttagtc taggacatca
    17581 aacgatcgcc cttgtctatc ctgaaaatcc accggaaaat gtccacgctc ggattgaggg
    17641 gtttaaatct gctcttgatg tttaccaaat ccctcacgac caactgatcc tcttacctac
    17701 acagttctca aaacaaggtg gctaccagat cacagcagaa cttcttgatt ctgctgcaac
    17761 tggtgtgttt gctttaaatg atgaattagc ttttggatta tatcgcggat tagaggaagc
    17821 tggaaaaagc atccctgaag actacagcat tattggctat gataatatag atatgtgcga
    17881 gtatatcaag ccgaaactga caacgatcgc acaaccaatc ttcgaattgg gacaaacatc
    17941 tgccaagtta ttgttagatc ggatccaatt cccagaaaaa gaatgggaag aaaaaagatt
    18001 acctgtacga ttagaaaaaa gattttcaac tgcacccttg aaatagcttt caaggtgtgc
    18061 tatattttta tcgaactatt aaaacgtttt actaaaacgt tttaaaaaat aattttccca
    18121 tcaaaataaa tgagatagtt ggaggtttgt aaaatgaaca cggttacagt aattggaagt
    18181 attaatatgg acactaccct tcgtttaacc aacatgccca agccaggcga aacgatgcat
    18241 gcgcatgaga tttttcacgc tggcggaggt aaaggtgcca accaagcagt ggcagccaaa
    18301 cgttcaggcg cacggacatc ttttattgga ggcgttgggg ctgacagcga aggacaacag
    18361 ttattagatc tactgacaaa agaaaacatc gatacatctg ggatagctga aatccaaggc
    18421 gccacaaccg gacaagcaat gattatggta gacgcagcag gagaaaatag tatcttgatc
    18481 catgcagggg caaataatgc tttccatgaa caagaagtcc taaaaaacaa gcaactgatt
    18541 acaaacagtg actttattat tgctcagttt gaaagtagct tagatgcgac gatcctagct
    18601 ttttctattg caaaagacgc aggaaaaaca acgatcctga atcctgcacc agctcgtgaa
    18661 acgattccta ctgaactact tgaaaagaca gatatcatta taccaaatga aacagaaaca
    18721 gaaatcatta caggaatcag agtgacagat cataacagtt tggtcgcagc agccgaaaaa
    18781 ctccatgaat taggtattgg aacagtaatc attacactag gcagtgccgg cgctttctat
    18841 catacagaaa aagaacacgg tatcgtccct gcttttaaag tggatgccgt tgacacaaca
    18901 gccgcaggtg atacatttat tggcgcatta agcagcacac ttcagccaga ccttagtaac
    18961 ttgaaagaag cgatccttta tggaaatcta gcctcttctg ttgctgtgca gtcttatggt
    19021 gcgcaacctt ctatccctta ccgcgaagct ttagaacaat cagcagtcta aaattaacaa
    19081 tgagaggaag aaacaaacat gaaaaaagga aaagtaatca attcagatat ttcacgagta
    19141 atcgcacaaa tggggcactt tgataaatta agtatcgggg atgcaggcat gcctgtcccc
    19201 tttggtactg aaaaaataga tttggctgtc tcaaacggtc ttccaagttt tttatctgtt
    19261 ttagaaaacg ttttagaaga acttgaggta caatcgattg aattggcgga agaaataaag
    19321 acaatgaacc cggaaatatt ggaaaaaatc aaattattgt taccagatac accgattagc
    19381 tttatcccac atgaagaaat gaaaaaagag ttgaataact gtcatgcatt tattcgtaca
    19441 ggcgaaatga caccttacgc caatattata ttagtgagtg gagtggtctt ctaatggaaa
    19501 cgacaatgaa tcctgaacag atggtactgt ccgagcagat tcatatgctg attgaacagt
    19561 tgggaggaaa agacaatatt gattctgtcc ttccatgtgc gactcggctt cgtatcaaat
    19621 tgaaaaaaca gccagtagcc aatgcagaaa aattaaaaca gcaaatccct tccattaagt
    19681 atttcatttt actcgataga tatgcagaaa taatcatgga tcagggattg gctcctgctt
    19741 acaaggaatt gacgcgtcaa atcgatcatt cagctccgaa agaaaagcta aatccgatcg
    19801 aaaaactatt tggtttgata tcggaaatct ttacgccgat cattccgctg tttgccggtt
    19861 ctggtatatt aaaaggtctg gttgttttag cgactacgat tggctggctt agtgaaacaa
    19921 gcggaactta tcatattttg tctgctgcgt cgaatgccgt cttttatttt ctgccaatct
    19981 ttcttgcttt ttctgctgcc aaaacgttta aagtgaatgg atatattgcg gctgtcattg
    20041 ctgcagcact gattgaaccg aatctgacct ctttactttc agacagtggc aaagcagcta
    20101 ctgattttct ggggattcct gtagtactga tgcagtatac gtctactgtc atgccggcga
    20161 ttttaggtat tttcttttat tcttttgttg aaaaattcct caaaaaatat attcgcgaaa
    20221 atatgcaatt agtatttgta cctttacttt cattagttat cacggttcct ttgacgttaa
    20281 tggtattcgg gcctgcgggt gtttaccttg gcgaaggttt agctgccgca attacttgga
    20341 tgatggatat taacggaccg atcagtggcg caatcatcgc tggtggttgg aatattttgg
    20401 taatatttgg tatccaatgg gctgtaaacc ctgtcatgat cagtaatatc tctgcttatg
    20461 ggttcgaccg aatcgttccg ttgacaggag cagccaattt tggaatggct ggtgcagctt
    20521 taggtgtatt tctgcgaagt aaacgttcaa aaacccgttc gatctccggt tctgcttttg
    20581 cttcgatttt acttgcggga gtgaccgaac caactgttta cggaattgcg atcccattga
    20641 aaaaaccatt tgttgctgct tgcattggtg cagctgctgg tggagctgta atgggatttg
    20701 cccaagtgaa agcgatcgct tttgtatttg gtagtctgac cacattgcct gcattcattt
    20761 ccggtacttt tttctggtac ttagcaggac tggctgttag tctagtcgta gcaatgatca
    20821 ctactctcgt tagcgggttt gatgaagacc taatgtctta tgaatagtta atgaaaaaga
    20881 ctgaagtatg agatgtttat cacatatttc agtctttttc tttttaggat cattattttt
    20941 ataaattttt gtgatacttt ccaaactttg gtataattta catatgcata accattttta
    21001 tccccacttg tttatcccca aacaaaggtg gtctatgcaa taaaatgaac gtcaaagaaa
    21061 aacgaatgaa tgctcgttgc ttatttacaa attgttttat ttagaataca cagacagatt
    21121 tatgaaaaga aaaagctttt cgacacgatt atctgttcca aaaaatgtct aatgaatgct
    21181 gaataagaca gaaaatcaat gaaaggaaaa ggactagttt attatgaaaa ataaaaaaac
    21241 gatacgtttg ctagctgcta cttggctgat tgctccgtct gttttgtcga ccaataccgt
    21301 atttgccgta gataatcaaa tagctgatca aacagtcaca acagatacga ctacatccag
    21361 ctcagctact agtgaaacaa cggattcttt tacagaagac ccaactggaa caactaactc
    21421 aacaacagga tcgaccgagc caactgaaat tgatccggct gatccatcag aaaatgaaaa
    21481 gccagccgag acatattccc ttacaattgc tccttctttg attggtaaga tccaactgac
    21541 tgcttcgaca gcggattccc ctgaacagca acctttggat attacggaaa gtggaacggt
    21601 agaaggttta ttagcggaaa cgaaggtaac ttacactatt acgccagctg aaggtgaaca
    21661 aattgcttca gtcgctgtta atggtgccaa agaagtaaat tatgatgcaa agacctttac
    21721 agtaggtacg aacaatttat ctattactgc agaatttaaa gctggaccgg atacttcaac
    21781 agagaaacca actgatccta gtgaagaagt ccctagtaca ccagatccgg aaacgccaaa
    21841 caaaccagat gtgggtaata acaacaataa taatggttca aataattcaa acaataataa
    21901 caataattcg aacagcggga attcttcgaa tcaaacaaac ccatctggat cgaataacaa
    21961 tggaaatact cagagagaac caaaccatcc aaacaatggt tcatcgaatg gcggaacgaa
    22021 caatgcttca tctcgtccaa atacagcaaa tcaaagcggt atagagaatc catcttctgc
    22081 ttcttctgat tttgttgtaa aatcaccaat tgatgctgta ttgcctacaa atacaacgaa
    22141 tgtccaacaa gcaatcgttc gagaagcatt caagcatctt ggaaaaccat atgtatgggg
    22201 agcaaaaggt ccaaatactt ttgactgttc tggtttgact tattacgtat atatgaaagc
    22261 aaccggtcat tatatcggtg gctggacagg cgaacagcaa tatgcaggta cgcagattcc
    22321 agtaagtcag gcccaacctg gcgacctcgt cttctgggga ccttcttccg gtgttactca
    22381 ccatgtaggt atctatattg ggaacggaca atttatccat gcgcctcaac caggagataa
    22441 agttcgagta acttctattt cagatttcac gcctgatttt gctgtccgag taaacttagc
    22501 aggtttacct gctgcttcag gttcgctgac aggcggctca tctattttag atggattaga
    22561 tggctcgttc catttcagtc aaaaccaaac aactgatcag ttcttgaaaa agattgcaga
    22621 tgatgcacag gaaatcgggc aaaaagaagg aatctacgca tcagtcatga tggctcaagc
    22681 tatccttgaa agtggatctg gaaacagctt attatcaagc gaaccaaatc ataacctttt
    22741 tggtatcaaa ggatcatata aaggaagcag cgtgaccttc aatacacttg aacaagatag
    22801 ttccggacaa agctaccaaa tccgcgcaca attccgtaaa tatccttctt acaaggaaag
    22861 tttggaagac tatgccgact tgatcaaaaa tggactgact ggcaatcctg atttctacaa
    22921 accaacttgg aagtcggaaa caaaagacta caaagaagca accaaatatt tggaaggacg
    22981 ttatgctaca gatcgccaat attcacaaaa attgaacgcg atcatcgaag cgtacgacct
    23041 gacaaaatat gatgaaccga aaaaagaagc agcaacaact gaagaagcaa acgctgaaat
    23101 cctcaatatg tctaagcgat tcgatgtgcc aatcaaatgg acaacagatg gcgtgccaat
    23161 cacaggatta gaattttcaa cgataaaacg cgtcaacaac gcttcttcca tcttccgtgt
    23221 cgcttcaatc tgggatttgt ggaacaattt tacggcaaga cagataccag aaacaactac
    23281 tcgtacagtt aaagctgcaa ctgttcctgt actaaccttg ttaagtttag acaaatcatt
    23341 ctggcttgat ctgcctttaa agaaatagaa taaattaaaa aggggatgtg acactgtcac
    23401 actccctttt taatttattc tatttcttta tatttatttt gtattccgtt acaaactcgc
    23461 gtacttggtt aggttccaga gtgacatctc caaaactttc aagtcgctcc gctcctggca
    23521 gttcttgagt ctcaaatgtg ataccgccat gatttactaa tttattcccg cgcatttccg
    23581 gtccatcttc tccaaaattt gctgtaaaaa tcacgattcc tgaagcatct gtttttactt
    23641 ggatcgatac tttttcatct gggcttgtca attccgcagc atttagtaaa ccagattgtt
    23701 ttttcaacaa aaatggatga tcgatgccgc ccactatcat tgattggcga aaatcactgg
    23761 caaagacctc acctaatttt ttaggacgtt ggaagtcaaa tgctgtgcca cttactgaaa
    23821 ctacttctcc tgtaggcgtt gtatctttat tgagcgtagc aaataatcgg ctattcaacc
    23881 aaagtatatg ttcgtcgatt gactgtgctg gatcaccggt taaattgaaa tagacatgat
    23941 ttgtaggatt gaatactgtt ttgcggtcac tgatcccttg gaccgtcact cgccatgtat
    24001 catcgttcgt cagcgtatac cgtacttcta catctaaagt tccaggaaat ccgttttcca
    24061 tatctgggct agtctgatag aaaatgaccg atgcttcgtt ttcaccattg atgactgtat
    24121 aatgccactg cttggcttca aagctaggtg caccaccatg taaacaatga ccagtgtccg
    24181 ggtctgtttt tacttcgaac gtttcatcat ctaatttgaa tgtgccattc gtaatccgtc
    24241 cagccatacg tccgattgaa gcaccgatat aagggtcttt ttctagatat tcttttgctt
    24301 catcaaaacc taagatcaac tctcgttttt cattatcgat tattgtttga aaatgaacaa
    24361 cccgtgcccc aagatcactg acagacaaag atgtcccatt ttgattcgtg atcgtaatca
    24421 agtgatagtt ttcgccaaaa ttttgttcat tgatttccat gttccttgca ccctcttttc
    24481 tttcatttgc actgattcca gtatacatgg aaaccgtttt cttaacagct tattccgtga
    24541 gtaacgagaa aaaatttact atattctaat aaaagaccgt gaaaaaaagg ttgtgacaaa
    24601 aatattgtca caacctcttt ttccgaacaa acggtgttca aaagcttgca tctgcggtaa
    24661 taagcccaac caaacgaaaa atatgaggag ctatttttgc ttggctgttc ttattacttg
    24721 atgcaggaca gctttttcac aatctctttt tcaatacgat tccagtatat aaccatgtcg
    24781 tcagtacata gtagatgaaa tacaatacga agaatatcgt aaacggaacc caaatatctt
    24841 tgtatggatt ttgcatgaat agaccaaaca tttgtaatcc gaataagaca tggactacac
    24901 ctaagattcc tggtgctaag aataacacgc ctacttctct acggatcgat tgtttaagca
    24961 acccgcgtcg tgttcctatc ttttcaagca tttcataacg agcaatgtca ctcttgcttc
    25021 ctgacaagat cttgaacatc aaacagctag caagcatggt caagaaagct aaacctagga
    25081 agaatcccat aaattcaaaa ccagagaata gaccattata cacttcatac atttcatatt
    25141 tctggttgct gtactctact ttttgcaaag atggattgtt cattttattt tgagcaacaa
    25201 gtgtttcgat tggtttcagg tttgcttgga agtcttttac ctgaactagc tgtaaagtgg
    25261 attgtggcaa gttcagttga tcaaattctg attgactgac taccttgatt tctttttctt
    25321 gctgttccgg caattcatag cttcgaagtt cctcttgtac agtcagttca ttcatttttt
    25381 tagcaggaac tttttctttc gtgatcttcg gcggtgttcc atcttcatgt tttatcatga
    25441 tcaatggttg agccgcaaat tcacttgcat tatagtaaat cgttcccgca tcttcttttt
    25501 gagtataaga agcagaaagt gttggtgaaa gttctttgat tttttgctga tcttctgccc
    25561 gaggattatt catcactaga tcatatccag aattgttttc tgtcaagtct gcaatttcat
    25621 ttttaaaacc taatcctacc gtcaaagcgc ccagtgccaa ggcaaacagc atagcgacca
    25681 ttgagagcat ttgtgtatat tcacgaatac gaaagtttaa ctgtgacaat gtgaaattgt
    25741 tcaatttttt caatgaaatc tgttctgatt tctttagtaa tgataaaaag aaaatgatca
    25801 cagaatggaa aatgaaataa gttcctaata cgatcgtaac taaggctacc cctataccaa
    25861 agacttgata ggtccccacg tgtttcatca tatagtaacc gcagcctaga aaagcaattc
    25921 ccagtactac ttcaaggaaa aacaagaatt tatgctgctt gattcttgtt ggtgtctgat
    25981 ccgcacgaat caatgctaaa atcggttttt tgacaatcga aaaagcattg acaacagcag
    26041 ctaataaaaa caaaacagca aaaaacaaaa cagtgatcaa caacccgtga atactaaaag
    26101 gtgtaaaatg cgaaatattg agatctaact gatccactaa taatcggtgg acgatacttg
    26161 tcaatcctac accgacagct gaacccacta aagtagctgc gatccctact aagaatgttt
    26221 cagaaaaaat catctgtgcg atttttctgc cttttgctcc cagcatcata aacattgcat
    26281 agtctttttg ccgcatcgtc atcaaaaagg aatttgcata taaaatataa acgaatgtga
    26341 tgatccctag taaaacagaa ccgaatcgga aaatgataac tgtagccgca acgatcgtat
    26401 tacttttcaa aaaagcttcg ttgcttgcca ttgattcaaa catgtaaaaa atagcagatg
    26461 ccataactaa tcctgagaat aacaccaagt agtctcttaa acggctcttg atcccagtaa
    26521 gtgataattt ccaaagcata gtcaatctcc ccctactgtt ccaagtttcc aagaatagat
    26581 agtatttctc gatagaagga atcacggctt tgatctccac gtttcacttc ttgatggata
    26641 ccgccatcct tgatgaataa aatacgttgg caataacttg cagaaaacgg atcatgcgtc
    26701 acaagaagaa tcgatacatg atcttttgta ttcaattcgt ccatcgtatc taaaagatcc
    26761 cgggcacttt tggaatccag cgcacctgta ggctcatccc ctaatagaat cgttggttgt
    26821 gtgatcaatg cacgtgcggc ggcgacccgt tgtttttgtc caccggaaat ctcagaagga
    26881 tagctttcca aaatatgaga gatggataaa cgttctgcaa ttttttggac acgttgcttg
    26941 atttcttttg gtctgacgcc ttgtaaagaa agaggaacag cgatattttc ttgtgctgtc
    27001 aagctttcta ataaattgaa atcttggaaa ataaagccaa tctcattcgc acgaaaatct
    27061 gctagttgat ttccttttaa tgtggtcaca tctttttgat tgattcggat atgcccatct
    27121 gttggtttat ctaatgtgga taaaatattc aaaagtgtcg acttaccaga accagaagcg
    27181 cccatgatcc caataaattc gcctttttct acatcgaagc tgatgccgct taatgcttgc
    27241 gtctttttct cattgttttt tccgtatact tttgttacat tctgtacttc gactattttg
    27301 ttcatttctt tccctaccct ttaattagtt tttccagtgc tgttttcggc acgtcttttt
    27361 tttcgacttc ttcatacgaa gtgacacctt tatttttgtt ccaggtgatt tttaaatatg
    27421 ctcctttacg aagcggtcga gtcaattgtc ccttgaagtc cagttctttt tccttaccct
    27481 ctttcgtata gccaggcagg atatatctat agttgactgt ttcttgtcca gagtctgatt
    27541 ttgaaactgt ttttgtacca tctgtcgtaa tttgtacata gtattcttct ccacccataa
    27601 ctacgtaatc tgcgattttc acccctgcaa gtaccacaac gatgaaaaca agtagaccga
    27661 tcagaagttt tttcactttt tccccttctc tcttacttta tatactgatt atagaaaaaa
    27721 gcgaacaatt ctgccttcga atcaactatc attcttcttt caattttgtc attttcttta
    27781 aaacaactaa aaaaagagtt caagacaata atccgtccta aactccttct tcctatctta
    27841 caaaaagtat gtcacttacg gtctttcatg tcgcctgtat tgataacagg ttgcgttcct
    27901 ttgtctgtaa tgattgagac aagtaaatta ttgatcaatt ggactttccg atcatcggtg
    27961 aaattgattt cttgtccatc ttcgatttgc tctaaggcca tttgagtgat tgtgacagct
    28021 ccttcaacaa tggtttgtcg agcagacaaa atggcttttg cttgttgacg ttggagcatg
    28081 gcactagcga tttctgttgc ataggctaaa tggttcaagc gtgtttcgat tacttcgaca
    28141 cctgctactg ctaaacgctc ttgcagctct ttttgcaact cgtcagacac tgccgtcgtg
    28201 tttcctcgaa gcgtcaaatc attctcattg aatgtatcat acgggtattg gctagcgata
    28261 tgccgaatgg ctgtctcact ttggatttct acaaaatctt gataatacgc tacatcgaat
    28321 aatgcctttg ctgtatcaac gactttaaag actactactg ctgagatttc gatggggttt
    28381 ccatccaaat cattgacttt taaaacagaa ctattgaaat tccgaacttt caacgaaaca
    28441 gtcatttttt gtgccaaagg tatcgtaagg aaaaagccat tttctcgaat cgtacctaaa
    28501 tattgtccaa aaaacaaaat tacttttgct tgattaggac tgactacagt tgccgaactt
    28561 aacaacaata acgtgatcaa ccaaagaaaa atacttaaaa aaagtgccca aactttcgct
    28621 tgccacatac ctaaatagaa gaaaaacaat ccaactaaag ctaatacgac caatccgaca
    28681 atccctgcat aaccattgat ataaaatgct cttttttctc tcatgctact tcctgcgctc
    28741 ctttatcctt caataaatat ctaatgttac ttttttaatt tttttttcga atacgcgcca
    28801 ttacaccttc tagttctaac tctttgtctt cagtgatgta ccaaccgctt gcctgtgtcc
    28861 ctccggctgc ctgcggtaaa taatccaatt ttgttcgaac ccagtgcttt tcttctgtaa
    28921 aatagtcttc taccaagacc tctagatcca tccctgctga aagaggcaaa tcttcccgaa
    28981 cagatttaaa gataaatcgt tccttcttcc catcatactt caatattcct gtatcgataa
    29041 tcggtaaaga ataataatcc agctctttct tcaccgtcaa taaatgtttg aaaacttcat
    29101 ggtacacttg ctctaattgg cgaccttctg aattgttcgg gtaattaacc atcacttttt
    29161 ctttgtctgg atcatagaag gtttggctta gttgagtgat ataagtatgt aatctttcca
    29221 tttctgtttt taacttttct ctttgacctt cttctacctt catatactct ttcaccctgt
    29281 tttcttttta gatattttct ttcttatttt actatagtta tgtgcgttaa gcgatgaaaa
    29341 acagcaaaga ttggaaggat ctttgctgtt ttttcttaaa atatccagtt gagtacagga
    29401 tcaatctgtt tcattgtcct cttgatccgt cgtaatattg atcgatgtgt ctttagcagt
    29461 gatctttagc tttttctgtc cttgttctaa caaagtattt gttattggct ggtagtcttt
    29521 ttcaatataa gattcaccag acgtttggat agtatagcca ctgttttcca tctcatgcaa
    29581 agataaattg ccaccgttta ggatgacttt tccttctttg ctgatcaaac tgtcttctag
    29641 atggaagttg ctgttttcac ctgtgacgat caagttatcc acattggcct catctaatcg
    29701 aagtgagcct aattcatctt tcgcagttat tgttcctcct gtaaattcat tgaacgatac
    29761 ccagccatca tgtgtcttag tattcacttt tttgattgat acttcgttga tatcaattgc
    29821 actattattc ccttcgattg tcaattcaga taattcatga tctgcaggga caacaatagt
    29881 gatttgtgca cttgtgtctt ctaaactaat gattgcacct gttgattttt tgactgcatt
    29941 cacagtcaac acgccatttt tctcctttat ttctggcttt tctgtcttat cataagtcgt
    30001 ttttatctca aaatccaatc cgcgtatgac cgaaactgtt tgattttctg catggatgac
    30061 gattttgttt gcatccttga ttttttttgt ctcagtaacc atatcgatca accgaggacc
    30121 attatcccaa gtcagactct tttgagcgcc taatgtataa gttgcacctg ctaggaggcc
    30181 accagtaagc atcagaccaa taccggtaat caaaaaatgt ttcaatctca atttatgtgt
    30241 cctccttcaa acgcccgctg aatgcagatc tctttttgta ataaaacgtc gtcccatcca
    30301 tttaacaaag ttcatcatga cataaaaaag cccttttgtt cctttcagca acagtggcga
    30361 aataaatact gcgcttcctg tggcgaacag accgacaccg ataaagaata atccactcag
    30421 aatcgattgt ccaaacacac tgattcctga gatgatcatg aataggccaa cacctaagat
    30481 actgatacat aaaacataga tactaaaaat gagcgctagt aaagcaacca atagtccccc
    30541 aataaccaat ataaacccca gtgctagtgg aatgaggaca ggagaagcaa ataaaccgat
    30601 aatgattaac caaatcaggc gaatttgtct ttttggattt ttcactaact cgttttctgc
    30661 ttgttcttct tctgaaaagt aactgatttt taattttaag gcaaactgct ttggtgtacc
    30721 atattcatca atgatctgat ctaatgtttt tccagaatct aaaaaatatt cctcataata
    30781 ctggatcaaa tcaaaacgat cgtcttcagg aatttcttga agatgatcca caacttcatt
    30841 caaatattca ttcatttgtg ctctcctcca ataaccggtc taaagaatct cgaaaaacaa
    30901 tccattcatc aataatttct tctaaatgac gacttcccat tggtgtcaac gagtaatatc
    30961 gacggtttct gccttcaaaa ggttcatcat aggtctcaag atatccttct tttttcaatc
    31021 ttctcaaaac cggatatagt gtagattcac tgacagagag actttcttgt acgatttttg
    31081 ttaaggcata cccatacata tcttccttgc ttaatgctgc tagcacagta ccatcaagaa
    31141 gaacagttgg aatctggatg tgcattacgt caccttctat tctcaagtgt aatattttat
    31201 ttcaatctat attatacaac atataatata gtttcaaaat cagtttgtca aatacaaaaa
    31261 aactgactaa aagatttgat cctttttagt cagcaaaaaa tggcttctct ttgattttta
    31321 ttataacagt ggcataatcc aatttgtcca catccagctc agtggcatga taacgatcat
    31381 tgcaggtatc atgtcagcta ctgggaacat tttcacgttg atcattctaa aaccggtagc
    31441 gaccatcaag aatccgccgc aagctttaaa atctaagatc atgtctggtg tagtcaatgg
    31501 ataaataaat tttgccaata agaacaaaat ataaaaaata atgaattggg gaattgctat
    31561 cacagaaacg acatacccta aattacacgc aaaaatagct gctgtgaaaa aatcaaggac
    31621 agatttagaa atcaaaatcg tactgtctcc tgtcatccca gaatctaaac ttccatagat
    31681 ccccgtacca cttgcacaaa atagaacaat gaccgtcact aatgtgctga taaattcatc
    31741 gtgggtcata ccaagctttt cactagggaa gattttagaa ataggtactt gcatcagcgc
    31801 agccccctta tttatcccat tacctaaatg aacaatcaaa ccaatcgctg ttcctatcac
    31861 taaagcaaaa ataacagccg gcatattttt catcggcgca atggaataga ttcccatccc
    31921 catcgaacac acaccaaaaa ccatcgtcag ctgtgcttta aaatgttcac ttaatttatt
    31981 tcctactaat ccgccaatta gtccgccaaa caaaacggaa cctacattga tcaaaactcc
    32041 agttggcatc aaaaaactcc tctctttttc tacgaagcga aaaaaatcag aaaaatttct
    32101 gtaattattg ctttcgctgc aagaaagatt gtaacagaca gagaagatta caccaattca
    32161 ttttttgtgt taaagtattc tttattggaa taagtaggag gaatatgatg gatatccata
    32221 aattcgaagt ttttcttgat ttagccgaaa caatgaattt caccaaaact gctgatcgac
    32281 aattcaccac ccaaggaaat atttctaaac agattctttc gttagaaaca gaattagacg
    32341 tcaaattatt cgaacgcgcc catagaaaaa tcgaattgac cgaagctgga agcttattgc
    32401 tcccatatgt aaaaaatgtc gtagaacaat accatgctat gcaagaagtg cttgaaacct
    32461 atacgaaaga caacaatctt tcattaaata tcttaacgat accgacaatg atcaattata
    32521 aaggtttttc caaaatcacc gagtttttga aacgccatcc tgaatttaca gtccagttga
    32581 aagaagtgga aagtgtcgaa ttaacttaca atgacgccaa tgaacatagt gacacgatcc
    32641 actttgctag aagtttccag gccccaccaa gttcgattga gtggttgcct actgaaacag
    32701 acgattttgt agcagttcta cctaaaaatc atcctttagc tagtaaaaag aaattggatc
    32761 taagcgaact gaaaaaggag aactttttgc tccttggccc cactacgaac ttgtatcaac
    32821 ctgtactaga tctgtgtcgc gaagctggat ttgaaccaaa aatctcttat aaaggagcaa
    32881 gaatcgactt gatcatcaat atgattgcaa atgacatggg gattgctatt gttatggaaa
    32941 aaacagtcaa gaatctatta aaagaaaata ctgtgattct ccctatttct ccaacaacag
    33001 agagttatct tgcatttact cgtaaagtcg gtgagcattc tttggcttca gatacatttt
    33061 gggagtattt aaatcaagaa gaaaactaag ccctacattt tctgtccatc tatccaccca
    33121 aaaaaagaga gcgacgcatg actttcgctc tctttcttgc atcatgtcct tgtttcaatg
    33181 acacctctaa catttctcgt agttcaataa gaaagcagcg atttttatgc caaaagaacc
    33241 gtaagaacat ttccctaatc acaaaaaaac gactaaaaac ccctaaaaac cttcaataga
    33301 atttttaggg gttttattct ttttcctatt gtttacttaa ccgtatcatt gctgtcgttc
    33361 ctactccttc gatactgcta taatcaatac gtccatcatg cagttctaga atttttttaa
    33421 ccagtgaaag tcctagccca ttcccagaaa tttgacgact tgtatctcct tgataaaatt
    33481 tttcaaacat tcgatctctg gtctcttcat tcattcctac acctgaatcg gtcacttcca
    33541 gtacaatctc tgtggctgtc tcaaatagtt tgatatgaat ctgtccattg atttgattgt
    33601 actttatggc gttatccatg atattcagcc acacttggta caaaaactct tcattgcctg
    33661 tgtaatttac tgcagcaagt tcgatatcca attctagctg ttctttctcc catttaggct
    33721 gaaaaaaaac gattacttct ctgatttgtt catctaatcg atactctttc ttttccagtt
    33781 ggatacgctg gttttccagt ttgttcaatt ttaagatgtt ctctgttaat tgtgaaagct
    33841 gcgtgatact ttcgataatc cgatataaaa atatttggcg ttcttcgtca gagagatttg
    33901 gggcttgtaa caattgaaca tatccttgga ttgttgctag tggtgttttg aattcatggg
    33961 atacactgga cacaaagtca ttcctcagtg tctcgatact attgagttct tggaccataa
    34021 cgttgaaatc tttatacagt tgctgtactt ctgctacttt ctgctgttca tccattcgaa
    34081 tcgaaaaatc tcccgttgct accagactca tatttgtccc tagcgcactg attggatgca
    34141 ggatacgccg cccaacaaaa atcgatattc ctgtacccac gaccatactg aaccccgcaa
    34201 agacgaataa cgggtataac gttggttttc cagccgattc agcaggcgag atagaatgtc
    34261 gagacaataa gaaaataaaa ccgagaaaac acagcagtgt cactaagatc gaaatgaaga
    34321 caatactagc aaaatagatc cataattgtg aaaatagctt ttttttcatc tttaaataac
    34381 cgccttatag cctagtcccc gtactgtgat gatttgaaaa tcttccgtct tttcaaaacg
    34441 ggtccttaaa cgtttgatat gtacatcgat cgttcgttct tctgtattcg tatctaatcc
    34501 ccaaatatca tccatcaact gttggcgagt gaagatcttg ttgggataag acagtaattt
    34561 atacagaagc agaaattctt tttgaggcag aatcaacgaa tgctgatctt gtaacactgt
    34621 ataagcatct agctctaatt ttgtttgtcc gattatcaag ctactgctgt aattgatctt
    34681 tgaacgtcga agcaatgctt ccacacgtag gatcatctca ttgacatcga tgggttttgt
    34741 catataatca tctgctccta atttgaaccc gcgtttctta tcctcaaaat cggcctttgc
    34801 cgtaatcatt agaacaggtg tgtcaaatct ggtgctacgt aatgagtcta gcaagtcata
    34861 gccatccatt ttcggcatca tgatatctgt aatgattaat tctatacgtg tcgcttctag
    34921 ttttttcagc gcatcctctc cattcaatgc ggtgatgact gaaaagccca ctcgttccaa
    34981 cacagaacgg taaagcaact gtaattgcgg atcatcttct actactaaaa tacgattcat
    35041 tgatctccct ctgttttttg aaaaatttct agattttttc ttgtagttca tcttcagtta
    35101 acatttattt cgtatactac gatcattgtt ctgatatact tattagtata ccgaatattt
    35161 caatacggga gaagggtgat tatgcttcaa ttaaaagata taaaaaaata ttataaagtc
    35221 ggcgaaacca ccaccaaagc attggatggt gtgtctgtcg ctttcagaca aaaagaattc
    35281 gtagcgattt taggtcctag tggttctggt aaaacgacta tgctgaatgt gattggtgga
    35341 ctagataatt atgattccgg tgatatggta atcaacggaa agtctactaa agactttaag
    35401 gacagtgact gggatgccta ccgaaacaac tctatcggtt tcatttttca aagttacaac
    35461 ttgattggtc atctaggcat tattgaaaac gttgaacttg ggatgacatt aagtggtgtt
    35521 tctaaagacg aaaaacgaaa acgggcagaa gatgcattgc accgagtcgg cttgacaaat
    35581 catatgcata aaaagccaaa tcagttatct ggtggacaaa tgcagcgagt ggccatcgct
    35641 cgagcactag ccaatgaccc agatatcctt ttgtgcgatg aaccaaccgg agcgttagat
    35701 acagaaacaa gtatccagat catggaattg atccaagagt tatcgaaaga aaaattagtg
    35761 atcatggtca cacataatcc tgaactggcc catcaatacg cagatcgaat cattgaattt
    35821 tctgatggta agatcttaac agattcccat cctcacatcg aacgaccaaa agatgatcaa
    35881 tttaacttac gcagaacaaa gatgagtttt tggacagcgt taaagctttc cttcaacaat
    35941 atccgaacaa aaaaaggacg tactttcttg acctcctttg cttccagtat tgggatcatc
    36001 ggaatcgcga tcgttctgtc cctttcttct ggattccaaa aacaaatcga taacacgcaa
    36061 gctgaaacga tggcgaaatt tccgattact atttctaagg taacgactaa ccagactcgt
    36121 gacgatgccg gattgggtgc aagcaaagcg gattatccag acagcaaaac gattactgca
    36181 aaagtcagcg aagaagataa agcacagcac acgaataaga ttgatcaaac atacgtcgat
    36241 tacgtaacag acatcgatcc taacttaagt aataatatcg gatttacccg tactacagga
    36301 atcaatcttt tacgtgatgt aaatggcaaa gtacagcctg tcagcttctc gaatcaaaat
    36361 cctgatacgg aatctctttc tttatctagt acgatgtcag caatgacagg tgtgggagtg
    36421 tcaagtttcc ctacacagct tgacactagc aaagaaaact ttttaaaaga caattattct
    36481 ttacttgccg gaagttatcc agcctcagca acagatgtcg tgctgatcgt agacggcaat
    36541 aataatacta atatcaacgc attgaagaat cttggatttg atgtgaaaga agacgaaaaa
    36601 cttgattttg atgatatcgt tggtacaaca ttcaaattag taaacaacaa tacttactat
    36661 acaaaacttc caactgggaa ttttatccct aatacagatt atgatgcgat gtatcagaat
    36721 gcttctgatg aattgaagat ctcggggatc ctacgcgtga aatcttcttc tacaatgaat
    36781 ctcttatctc caggaatcgc ttatagcgat caattaacga ctcaaatcgt caacgaaaac
    36841 aaagaatcag aaatcgtcaa agctcaaaga gacagtggtg taaatgtctt aactactgaa
    36901 aaagttgacg aaaatgccaa acaaacattg cttagttatt taggtggaga cagcttgcct
    36961 tcaagtatca tgatttatcc gaacaatttt gaagacaaag aaaagatctt ggattatcta
    37021 gatgattaca ataaaggcaa atcagatgaa gataaaatca tttatactga tttagctgga
    37081 acgatgactg aactgactgg cggattgatg gatgctatca cttatgtcct tattgctttt
    37141 gcagggatct ctttagtaac aagtatgatc atgatcagta tcattactta tacatctgtg
    37201 attgaacgga caaaagaaat cggtgtacta aaagctttag gtgcacgtaa aaaagacatc
    37261 actcgcgtat ttgacgcaga aacgtgtatt ttaggtattt cttctgggat attaggtgtg
    37321 tttattgcat ggctagctac gttccctatc aatagcatct tatacaacat gacggatcta
    37381 aaaaatgttg ctcaactaaa tcctgtccat gcgattattc tagtgatcgt ctctactata
    37441 ttgacgatgc ttggcggtca tcttcctgca cgaatggcag cgaaaaaaga cgccgctatc
    37501 gctttgcgtg cagaataagg ttgtgacaac cacatttaga tttgagtcat aagagcgctg
    37561 aaatagtaga aaacagcaag aaacgaggaa gtaatatgtt ccttgtttcg cgctgttttt
    37621 tttaattatt ttttataatt ctggtatact tttctttcct ttgtaccaca actcttctgg
    37681 cgtatacctg tcttttccat ttatgaatac tgttccatct gcttcaatcg ttacttcagt
    37741 cccaatccac atattacgag aaagtgcgag aagatttttt ctttttcctc ttcctttttt
    37801 tccaaattgc aaggcatcgg ttatctcttc tgtccaatga gatttcacgt attttacttc
    37861 tccgttttca aatgtaactt tcaaaatatt cgctatattt tccactttta ctgctttagg
    37921 aaatgactcc tccatttttt tcactttcct tcctccaaca ctaagtatgc gctttctttt
    37981 agtgataagt atatcatagt ttattctatt atcaaaaaaa cgcatccatt ccattgtaga
    38041 atactttacc gaacaagttg aattgttttc ctctttttca ccttttacta tagataggta
    38101 aagacaacta aggaggaatc tctcatgaca gatcaaccgt atgatttaag aaaagtcatc
    38161 gaagaactaa aaacacttcc aggtcaatat cacgaaacag atattgaaat cgatcctgaa
    38221 gcagaattat ctggtgttta ccgttatatc ggtgctggcg gtacagtaaa acgtccgact
    38281 caagaaggac cagctatgac tttcaacaat atcaaaggtt tccccggcac acgtgtgaac
    38341 atcggaacaa tggctagtcg taaacgtgta ggtgaaattt tgcaccatga ttatcgaaca
    38401 cttggtcgtt tcttgaaaga cgctgtagaa aatccaatca aacctgtgaa agtagacaaa
    38461 gcagacgctc cagcacaaga agtcgttcat ctagctaccg atgcagactt cgatattcgc
    38521 aacattttgc ctgcacctac aaatactcct gaagatgctg gtccatacat cacaatgggt
    38581 gtagtcttag gatcagatcc agaaaaaaca atgagcgatg taacgatcca tcgtatggtt
    38641 ctagaagata aagatacgat cggtatgtac atcatgcctg gcggacgcca cattggtcac
    38701 ttccaaaaac aattcgaagc cttgaataaa ccaatgccta tcactatcaa tattggttta
    38761 gatccagcaa ttgccattgg cacgactttc gaaccaccta ccactccact tggctacaat
    38821 gaactatggt gtgcaggagc tttgcgtaac gaacctattc aaatcgttga tggtatagca
    38881 gtcgatgaag tgggtattgc tcgtgctgaa ttcatcattg aagcagaaat catgccaaat
    38941 gaaacaatgc aagaagatat caacacgaac tcaggaaaag caatgccaga atttcctggc
    39001 tatactgggg atgctaatcc agctgtacaa gtagtgaaag tcaaagcagt tactcaccga
    39061 aaagacaatc caatcatgca aacaacgatt ggtcccagtg aagaacacgt atctatggcc
    39121 ggtattccta cagaagcaag tattcttgcc ttggtcgata aagctattcc aggaaaagta
    39181 gtaaatgtct acaatcctcc tgcaggtggt ggtaaattga tgaccatcat gcaaatccgt
    39241 aaagaaaacg aagcggatga aggtattcag cgtcaagcag ctattcttgc tttatcagcc
    39301 ttcaaagaat tgaagacagt cattttagtc gatgaagatg ttgatatttt cgacatgaac
    39361 gatgtagttt ggactttcaa cactcgtttc caaggagata aagatatcat cgtgatccca
    39421 ggaatgagga accatccatt ggacccatca gaattaccag aatataatcc tggctctatt
    39481 cgtttccgtg ggataagtgc aaaaactatc tttgacggta cagttccttt cgatatgaaa
    39541 gaacattttg tacgtgcaaa attccaagaa gtagaagatt gggaaaaata tttgaaataa
    39601 tgaatgatct gcaactgttt tgacatcata aaaaggagtg cggaaaatgg aacaattggc
    39661 acaacggaag aaaaaaatcg tgattggtgt aagtggcgct tcaggcacca tttatgccat
    39721 caacctagtg aagaaactaa aagaatatcc gatgatagaa acacacggaa tcatcagtcc
    39781 atgggcaaaa cagaacttaa agctggaatc cgacttatca ctaactgaat tcgaaagcct
    39841 gtttgattac cattattcaa acaaagattt aggtgctgct gtcgccagcg gatcattttt
    39901 gaccgatgga atgatcatcg taccggcaag tatgaaaaca gtcgcaggta tctctgttgg
    39961 tttaggcgac aacctgattg cacgtgctgc tgatgtaaca atgaaggagc aacgaaagtt
    40021 agtcattgtt cctcgtgaaa cgccattaaa tgccattcac ttggaaaact tgactaaatt
    40081 agcacgacta ggtgtgcaaa tcataccacc aattcctgct ttttataatc agcctcaaac
    40141 gatccaagat cttgttaatc accatacaat gaaacttttg gatcaatttg aaattgtaaa
    40201 tgagcattca aaacgttggc agggtgatta atatggaaaa agaaacgata gttgaagtaa
    40261 ccaaactagg tttcacgatc caagctgctg ttaaagtaat cggtaaagac ttattgatca
    40321 ctttaacagg cggagataca ccacatatcg gaacagtcac tacattttct cgtgatacag
    40381 agatgcaaac tgtacgtttc cctagccacg atggtcggtt ccataaagac gatgtcttag
    40441 ccgaaaaaat agctgtgatc atcaaaactg ccgttccggg aagttgtacg atcacttccg
    40501 gtatccatgt tgaccatatc tccaatgcac aaatcaacgc ctcttttccg atgtcagaag
    40561 agttaggtta caagatttta gattggttgg aaaaaataga tttccatatc aaagaaccag
    40621 tttattataa agatggtgaa aaaccattat gaaagaagag gttgtgaaat cagcgttatg
    40681 acctgagtat gagagaaaaa aatggaaaaa tagcttctca tattttgccg tttttgagct
    40741 aataccgagg gacagctgat tgaacgccgt ttatcaagag gttgtgataa agccgcttcg
    40801 tttcgagtag taagaaaaat tttgtaaaca tgggtttcca catttttgca taaatttgac
    40861 ttactaccga gaaactggct tttgaacacc gtttatcaag aggttgtgac acgtcttttg
    40921 tcacaacctc ttttattttt gcttcactgt aactgctaca atatcaccga tttcttttcc
    40981 gatgttttta cgaatatctt ttcgtagtcc aaggatgtag cagactgaac catctggatt
    41041 tttcacgccc atattcacga tactgccttc atacggcaca ttgtcaaaag ttgcttggac
    41101 tttaactctt ccagcaccga attcttttct tatatcatac ggaaaaataa tatatgcccc
    41161 gcccttgccg acttcggaag catagatttt tgattcaaaa gaataaattt ccctcatttt
    41221 atttctccta ttttttacat ctagtatagc atagcaaaaa aaagaaggaa ctgtgacaaa
    41281 agtcttgtcg cagttccttt tttcgaataa acggtgttct cctgccaact cctcggtcac
    41341 aagtcacaat agtcaacaaa catgagaagc tgttttttga aattgctcct tgtgactcag
    41401 agttaaacgg caggctcaca acctcttcct taaacggtgt tcaaaagctt gcatctacgg
    41461 cactaagccc aatcagatga aaaatatgga gtgctatttt tatctgattg ttcttactgc
    41521 ttgatgcaga gcagcttttt cacaacctcc tacatggtgt tcaaaatatt attcgccttt
    41581 tgcttctttc actaattttg cgaatgcttg ttgttggaat tcagcagcta aggctttcgt
    41641 acgttgttct tgctctgctt tgctcatttt tggattttca gtcattgctt ttaccatacc
    41701 gtcttgaaca taagcttttg cttcactttc catcgtactt tgctgtttct tcactaattc
    41761 agctaacttg gctgcttggt ccgtacttaa ttctacccat gtctctggga ttttgaatgt
    41821 attcactcgg ctgtcatatt cgtgattgtt atcagcaata ttgaaccaga atttatacat
    41881 gtcgtttttg tattcccaat aagtagtttt cgtcactttt tcagcatcgc cgtctgtctt
    41941 ttctacttta tttgtttcgt tatccgtacc agttgctttt ggtttttctt gtttttcatt
    42001 tgttcggtaa agatacactt tttcttttcc attccctaaa gctttataca aaagcatatc
    42061 tgctccttgg gaatcagcac tggaaacaat tttttctgtt tttgtttccg ttactttttc
    42121 cattccaaag tgattggaca aattagcgac gatcaaactt agtgaagcga cgaagattag
    42181 tccgaaaatc aaagataaaa aggtttgcca tgttttttta gcgaacacgt tcgttaaagc
    42241 aaaagcaagc aagctgacaa tcaaaactcc aataatcatg cattatcccc cgctttcatt
    42301 agaacaggtt cttttttctt caataaaaag gcaataataa agccaagaat cccaaagatg
    42361 atcgctacga taaacgcggc atggtaacct gacagtgtgg cattgattgc ctgatcttta
    42421 taagctaatg gcgtattatc caatacagat ttgcttggga tatttgcttt tgtcacattc
    42481 gtcaacacac tgatcaaaat agctgtacca atcgagcttg cgatttgacg gaatgtatta
    42541 tttaccgctg tcccatgact gatcaagtta tttggcaatg cattcatacc agaagtcgtt
    42601 accggcatca tgaccatgga gatcccaaac atacggatcg cataaaggat cacgatatat
    42661 aaaactggag tcgtttttgt caagaaagca aatggtgcag ttgccgctgt caaaatgaac
    42721 atccctgtga tggctaaacg tttcgcgccg tatttatcga aaatcgcacc cgtcacaggc
    42781 atcatagccc ccatgatcaa tgcacccggc aacaacatca agccagaatg gaatgctgat
    42841 tctccacgaa tattttgaat atacaatggt acgaccattt ctgcccctac catcgccata
    42901 tttgttacac cagctaaaac agctgcaatc gtgaatgtag atgatttgaa tacacgaagt
    42961 tccaagaatg gatgttccaa atgtaattga cgccatgaga acaaggcgat gaaaatcaca
    43021 ccaacaacta agaaaccaat tactttcaag cttccccagc cgtcatctcc tacactagaa
    43081 aatccgtaaa gtaaactacc gaatccaatc gttgataaaa tagcggataa aatatcaatg
    43141 ctaggatttg acaatggaat aacacttttc attaggaaga atgctaaaac caagacaaga
    43201 acaacgattg gcagtaccat accaaacaag tcacgccaat ggtagttatc aatgatataa
    43261 ccagataatg tagggcctaa agcaggagca agtccaataa cgatccctga catccccatt
    43321 gctgatcctc ttttttctgg cgggaaaata gatagcatga tattttgcaa taatggcatc
    43381 gatactccca caccgcaagc ttggatcaaa cgaccgatta gtaatgtact aaaattaggt
    43441 gctgtaaagc aagtaatcgt tccgatcaaa aagatcgtca tggcagaaag atataaattt
    43501 cttgaactga atttattgat cagccatgct gttattggga tcatgatccc gttgactagc
    43561 aagaaaccag tcgttaacca ttgcacatca gacgctgaaa tatcgaaagc tttcatcaat
    43621 gtaggaaaag ctgtcgtcaa taaggtctga ttcaaaacgg tacaaaatgt accaattaat
    43681 aagacaacaa ctaaaagtgt ccgattatat ggttttccgt aaatatctac gggttggttg
    43741 ttactcatat ttctccttct ttctgaattt tttctgaaac tactagaata ctttagtcct
    43801 aaattcgcta tttgtcaacc tacttgacac ataagttaat acggatataa ttaagggaag
    43861 caagaggaca aaaggagtga caaaaatggt tggggcttcc cttaaacgtt tgttggaaaa
    43921 tgatcaattg gttgttggca tcagtgaatt aagtgatatc gtaggtgtgt ctccacgtca
    43981 attaaggtat tgggaacaaa agggattcat tcgttcgatc gaagctgatg cgaattgtcc
    44041 tagaaaatat cgtttaccta cagtcataaa agtagaactg atcaaaaaat atttagatga
    44101 cggatttact ttgtcaaaag ctgcagaaaa ggcagaaatc cgccaaaaga aaatgcacca
    44161 tgtccgtaaa gttttttcaa aagcaatcaa agatatcgaa ttgatcgacg aacgctatac
    44221 gattttacaa atcggtaact tcaaagaaga gaatgctaag atgtacatca ttcatgacaa
    44281 cgagacagac gaattaactt acaagctcct tcctgtcgat gaaaaagctg actacgaaca
    44341 gttgttaaaa gaactctaaa cgccactaat ctcctaagta ccaagctttt taataagtag
    44401 atgctacata aatacttgaa aaaaggcctg tttgaacata agaatatccg aacggtgatc
    44461 tgcgtttctt tcatatagaa aggaattctc catcactgtt cggatatttt tttatttttt
    44521 tcggttactt tcttctaata catctgttag ccaatttgca tattctgttt cccgattgat
    44581 cgcatgcgct aaaatcaggt agtgtccgta attttcatgt ttttcttttt cagtaggaaa
    44641 gatcgttttt tttcgctcta atagatgaag tcgtttttct tcatgaaggt cacgctgctg
    44701 ctgaataatt tctactaatg ccggatcatt cacgtctttg acgaaataca atttcagaac
    44761 aaattcgtct cgattaaccg gtagttcatt ggtgggtgta tgaatccact ctgccaattg
    44821 ttccttccct tcttctgtta attcataaac tttcttttcc aatttttcac ctgtgatctc
    44881 tacttgatgc tgaatgattc cttgctcttc catttttgca agttcaggat aaatctgact
    44941 atgttttgct tgccaaaatt ctcctatttc atttttgaac gattgattca attcatatcc
    45001 tgtcatcttc ttcttactta ataaccctaa caaaatatat cgaagtgtat tttttcttgc
    45061 catcagtttt ccctccttga caaatcttta aatccattct atcataaatg agtaatcgaa
    45121 aacatgtagt ttattacatg ttttaattta taaggagatg cttaaacatg aaagaattta
    45181 aagaattgga agattttatt ggaactcact ttatctatac gtatgacaat ggttgggaat
    45241 atgaatggta tgccaaaaat gaccatactg ttgattaccg tatccatggc ggaatggtca
    45301 aaggccgttg ggtaaaagac caagaagctg atatcgttaa attgacggac ggcgtattca
    45361 aaatcacttg gacagagcca acagggacag acgtggcatt ggactttatg ccaaatgaaa
    45421 agaaacttca cggaatgatc tttttcccta aatgggttga agaacatcca gaaatcactg
    45481 ttactttcca aaacgaacat atcgcagaaa tggaagcagc tcgtgaaaaa tatgaaactt
    45541 atccaaaatt acttgttcct gaatttgcta cgatcactta tatgggtgat gcaggtatag
    45601 acaatgaaga tgttattagc gaagaaccat acgatggaat gccagatgat atccgtagcg
    45661 gaaaatactt cgatgaaaat tataaacgag taaaataagg ttgtgaaaga gaggttgtga
    45721 aatcagcgtt ctgacctgag tattagagaa agaaacggaa aaatagttcc tcatgttttg
    45781 ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca agaggttgtg
    45841 aaatcagcgt tatgctttga gtaataagaa cgctagttag aaaatagctt ttcatatttt
    45901 ctaggctagt gggcttatta ccgagaagca gccgtttgaa caccgtttat caagaggttg
    45961 tgaaatcagc gttttgacct gagtattaga gaaacaaatg gaaaaatagt tcctcatatt
    46021 ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta tcaaagattg
    46081 taacaactgc atattcacac agttaaatta gtgacttcaa aatgaaataa tgctcccagc
    46141 aaaaaattag gctgggagca ttatttcgtt attctgttca tcaaaaatca gcgttttgat
    46201 tccatttcat cacaagttcc tcctcattta ctgcttcatc tttttgctgt gtttgaattg
    46261 taaaaccttg tgaaagatag aaatgaatag ctgattgatt tttttgatag actgtcaaag
    46321 tcagtgtttt attggtttct tttatttttt tcattagttg cgtaccgatt ctttgttgcc
    46381 ggtattccct tttcacaaag attcctgcga taaactgatc aatcacccca gcaaaaccaa
    46441 taatttcttg atctatttct gctaagtaaa tcactgcatc tggtaaagct ttcctaacag
    46501 attctacttg attctcccaa taagttgatg gaatgaattc atgagaatcc aagttagcat
    46561 gcagccaaat cgatagaaca gattccagtt ctttggcaga caattttgtt gttgtgcgga
    46621 tcattttttc tctccttact tattcattga ttttaaaata aaaaccattt ttttgaagct
    46681 cttttacaag ttttcgcccc tcaaaaaagt atatcttctc ttgttttact tttctaatac
    46741 caaaagaaac agcaacttta ggatcatcag ctgaaacgat tcgataagtt ccaccaggca
    46801 attgatccag cttgactttc ccatgatggt cgctttgtac tgaaaacggc acaccatttc
    46861 tagtaagtgt ttttttctta tgtttcaaaa tgaatgaact accatttacc ggtttccctg
    46921 acgaatcact aataataaat gaaaaatcaa gcactttttt acttaatcta tactgataga
    46981 ttagtcgtcc gataaacact aacagtatga ctacagctat tatcgttcct gcaagaataa
    47041 accattgccg catggttctt tcctgatttc cttttccttc ttcttttttt accgattcgt
    47101 tataaggaat ccgatgtcct gtcactagca aacgatgcgt gttgatcata tacggcgtac
    47161 atgtaagcaa ggtaactaag tcttctcctt ctgaaatcgt tagaacagac gtgtcatctg
    47221 gcagtaccac ttgtatattt ttaacttgat aagccaattt ctcccccaaa acagtcaaaa
    47281 taaacgtgtc tccgttttct aaacgatcta attcggtaaa taaccgtcga ttaggtaaac
    47341 cgctgtgagc agagatcact gaatgcgtcg aatttccacc aagcgggaaa gaggttcctt
    47401 gcaagacgcc tgctccattt tcaaggatcc taccattgag ccgatcataa agtgaaatat
    47461 ttaattggat cttaggtata ttgatacttc cgatcagcca ttcctctaat tggctggatg
    47521 ttgtactttc atttctgttc ttttcattga aaggatcagt accaggattg aatccttggt
    47581 cagttaatcg ttggtttttc tcctccattt tctttcgctg cacttcaatt tcagctgcgg
    47641 tccgtttttg tacttgttcg atacgttttt gatcaataaa actatttaaa gaatccacat
    47701 aaaatggata taaagcgatc agtaacccta cgacgataca tattaataaa aacaaacgaa
    47761 acaaaagtcg acgagactgt ttttgcttac tctgcttcac atgtgtcctc ccttgcaagt
    47821 ttttttgttc tttcttttat tatatgggtt caggaaaaaa atagaaaatg aaagccgatg
    47881 aggttgtgaa aaagctgtcc tgcatcaagt aataagtaca gcccaaaaaa atagctactc
    47941 atattttttg ttgggttggg cttattaccg cagatgcaag cttttgaaca ccgtttaagg
    48001 aagaggttgt gaaaactgcg ttatgctttg agtaataaga gcgctagtca gaaaatagct
    48061 tctcatattt tctaggctag tgagcttatt accgagaagc agctgattga acaccgttta
    48121 ccgagaggtc atgaaaaagc tactttgttt cgagtattaa gaaaaattct gtttcaaaac
    48181 aaggagatgc aacaaaactt atgtcgcatc ctctctcctt agaactttca cttatgtttt
    48241 tcttgtaaaa ttcataaata ttttcatgaa ttttctaatt tcttgcctac ctctcgcata
    48301 ggatatatga tgaatttatc taaagtttgc aaaggagttc gacaattgaa aaaatatggc
    48361 ccatttttag caattttttt cattgctttg gcatttctca tgcctcattt attcacacat
    48421 aaactaattt tgggtgctga tgccccattc cattacaatc ggttctatga tgccgcgcaa
    48481 caaattaaat atggggattt ccattatttt ccttcccttt atggattcca gcaaagtggc
    48541 aggatcgtga atgccgttta cggcccattt ttcgcttatt tacaaggatt gatcgtgttg
    48601 cttgcaggta gctggtttca ttatcagatt ctttcaaatt tcattcttta tcttatttca
    48661 ggattttcta tgctgatttt actgagatat gtcaatgttc gaaattgggt aagtgtacca
    48721 gtagccgttt tattcatgag tacgtattct attcaatact ggtctatcaa ccaaggattc
    48781 actagctggg ggacttgttt ttatccttta tgtatgatcc ctattgctga tttcgtcctt
    48841 cataaaaagt tccctgttat caaagtcgct gtctcagtgg gacttatgac acagatccat
    48901 ttactgtctt cgatcatgtt agctttgatg tatatccctt tttatcttta ttactttttt
    48961 aaccagactg aaaaaggaaa agcactgctt caactgatta agtcggtctt gctttatttt
    49021 gttctgacag caaatatttg ggcaggatta gctattgttt acagtgggaa taagattctt
    49081 cctccctttg ttaacagaga aatgagtcaa aaggcgatag atctggatgg cagttattgg
    49141 ctccattacc ctaaggcctt tcctatattg atattatcag gttttttgct ttttattttc
    49201 tttcaacaaa agaacacctt attccaaaaa atcgtgttgt acacaagtct tgttttcttt
    49261 tttctttcta ctagcttgat tccatggacg acattgatcg aaaaaaatgt gccttttatt
    49321 agtttgatcc agtttccatt tcgatttttc gtgccattta cgctattgct tctactttac
    49381 cttgcccttt cactgaacca gtggtctgaa caaaagtggt tccgtctttt aagtatattt
    49441 ggcattctta ttttttcagc acagacgatc cagaacttat accagcatct ggaaaagtgg
    49501 gaaaatgaaa catttgtcag tcgacatact tatctattcg atacgccgga agaagcaagg
    49561 aaaacattct tcagcaaaga tatgtctacc agcttgttcg ttttccaaaa aaccacacct
    49621 gactatctgc caatctataa ggagacaaaa gacaataaat acgaccggta taacgaagaa
    49681 atactagaaa acgaaacaac ttttataaaa acacatccag gcggtctttt gacaatcaag
    49741 tggaaaaaca ctggggacaa aactgttcat atccctgtta tcgtctacga tcggactgtg
    49801 ctacagcaaa atggaaaaac attgactgat tatgaagtaa cagatatcgg gaccccaatc
    49861 gtgaagcagc aaaaaggaat caacgagctc acgcttcatt atcaaacgcc aatctatttc
    49921 tatttcattc tttctcttac tttgattggc tggtttacct tgctatgttt atttatttat
    49981 catagataca agcttttacg tgcttaggca aaagggataa atacatccga tcaaaaaact
    50041 ctccaaacgg tataatcgtt tggagagttt tattttaatc agttttttta tgttatatgt
    50101 tatatttttt attcgttcac atctaattcg atttccaccc cgtagtgatc actgatgatt
    50161 ggactatcga tcccatcaaa aatccgttga tattttttaa cctgcattcc ttcagggacg
    50221 aaaataaaat ctatacgcaa tttttctgta ttttgttccc aaccatctat tttcttttcc
    50281 acagtagctg cttttccttt ttcattcgcg acaacatacg aatcttgaag aagaaggcaa
    50341 ctttctctta cagtataata accttgagag tctactggat tattgaaatc acctaggaaa
    50401 aaaagtggta cgcgagtttc caaaaagtag ttttccaagt ttttccactc aaaataaaag
    50461 ccactttttt tatctgtcca ccaagaaaag tggcagctcg agacaaaaat gtcctgatca
    50521 tctatctttg ttttcccaat caagatcttg cgtgtctgat aatcttcttt cttttgaaat
    50581 tgggagacgg taaggacctg actttccaat gtttttttcg taagcagtgc atttccttct
    50641 tcatatttat cataaccgat gtggctcatt tcccaactcc aaaaatactc ttttccgtgt
    50701 tcctttaaaa gctgaactaa acggtaggca aaattatctt tacggatagg tacttgattc
    50761 tttaccggac agaacttcat tagcttcgca ggttctactt cttcagattc cagtaattgg
    50821 ttgatttctt gtaaagcaat cacttcataa tcttctaaca ggatcttatc tgctaattct
    50881 tgcaattttt tctctggttc tggctcaagc cagctatgcg tgttgagtgt catgaatttc
    50941 attggttttc tccttgtctt catttcgaaa caaaagggtt gtgacaaaat ttttgtcaca
    51001 acctctccag caaatagtgt tctcctgcta actcctcagt aataagctca atggaccagg
    51061 agctattttt tgtttggctg tttttatcac ttggtgcagg gcagcttttt cacgacctct
    51121 ccactaaaca gtattcaaac tgctgcttct cggtaataag cccaaccaaa cgaaaaatag
    51181 gaggagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc
    51241 tctccttaca ttttaatgga gccgatcact ttaccagctg tcactgtacc taattgcttg
    51301 atcactactt cttctacttt atcgtttgta aatacgatca tgatcgttgg atctttttct
    51361 tcgttttgga tcacatcaag atccatctca gctaatttcg ttcctgttcc tacttcttgg
    51421 ccttctttga cagcaatctc aaaagctggc tgattcattt ggactgtatc gattcccata
    51481 tggatcaaaa cttcgatacc atctttcgtt tccagcccaa ttgcatgttt gctagggaaa
    51541 atacttgtca cttttccagc gatcggcgca acaacttctc ttgatgttgg aataacagca
    51601 aagccgtcac ccatcatttt ttgtgagaat accggatctt ttacttcaga taaaggaatc
    51661 acttctccag aagctgcgga aactagttct tgttctatac ctaagtattg cttactacct
    51721 gcttgtccat ttcctgtaga aaccatttct ggctctggaa tctctgctcc tgattgcagc
    51781 agatcttcga tatctgattt caagacatct gcttttggtc cataaactgc ttggactcca
    51841 ttgtctttga cgatcaatcc catcgcccct gctcgtttcc aggcttcttc agtccctact
    51901 ttttctcgat catttacact tacgcgtaac cgagtcatac aagcatcgac atctttgata
    51961 ttttcttttc ctcccagcaa atgaatgatc tggaccactt gctgatctgc tgttcctgtt
    52021 ccgctgttag atacaacggt ttcttcactg tcattgtcgt aatttccgtt acgtcccggt
    52081 gtagcaaagt tgaatttttt gatcaagaaa tttgctaaga aatacgtaac aacaccaaaa
    52141 gcgatcacac acaacacgaa gttgataaga tctccaccta gacccgcttt gatagctaac
    52201 ggtgtccgag tcagcaattc gatattccca aatgaatgga cccgcaatgg caagatatct
    52261 gccatcgcaa atgcagcccc ttggatcact gaataaatga catataatgg cacagcagca
    52321 aacataaaca tgaactccaa tggttctgtt acacctgtta gaaagacagc caacgctgct
    52381 gaaaaataca ttgatttata tcttgctttc ttatcaggat cgacattacg atacatcgca
    52441 aaagccattc ccatcaagat acctgaagaa ccaatcattt gtcctacttt gaaacgagcc
    52501 ggcgtccagt tatgcaacac aaactcatat tgcgacatat cccctgcacc tttaagattc
    52561 actaaatcag tggcccaagc caaccataac ggatcttgcc caaatacttg tgtccccgcc
    52621 tgtgcgcctg ataaaatctc atacgatccg ccaagctgtg tatagttgat aggaatcgtc
    52681 agcatatgat gcaatccaaa tggcaatagt aaacgttcta atgttccaaa taaaaacggt
    52741 gctagaatcg gtgcgctttc ttgtgattct gcaatccata aaccaaaatt attgattcca
    52801 gcctgtacgt ttggccacac cacagcaaga atcaatgcca cgattgttga ccataagata
    52861 acaacaaacg gaacaaagcg ctttccattg aaaaatgaca aagcatccgg caattttcta
    52921 taattatagt acttattata agccattgcc ccgacaaatc cagcgatgat ccctacgaat
    52981 actcccatat tcaaagctgg tgcttccaat acacttgtaa aaaatccttt aaccatgatc
    53041 ttcgtaccga acagagtgtg ggtaaatgca tcttcattcg caagcatgtc acttgtgaca
    53101 ccaaagatag ccccagtgat ccgattgatt aaaataaatg agatcccagc tgcaaatgct
    53161 ccgcctgctc tttcttttgc ccaacttcct cctattgcta aagcaaataa aatatgaaga
    53221 ttaccaataa ttgcccaacc aatattttca attactccgc ccgttgtgac aagcggcgtc
    53281 caatcaggat tgatcaaggg aattgatttc cctatactga tcatcaaccc agctgccggc
    53341 ataactgcta ccacaaccat taaagccttt ccaaattttt gccaaaactc aaaactaaat
    53401 atctttttca ttccgtttag ctcctttacg caatcggttt cgttaaacgt attatagcgt
    53461 gcgttttctt ttttgtaaat gctttatttt ttatttttct gtctatacca ttctataaaa
    53521 cctataatac gtgtgttccc ttctataatt ttctcctaaa aatttatttt tttaagcttt
    53581 tttctatgaa atattttctt ttgtctattg acttgtgcaa tcggttgcgt ttattatgaa
    53641 cgtataaaac agctcattag gaggagaaaa atgaaacaga tcaaacgact atttgctatt
    53701 gatccatgga aaatcagcac gacacaatta gataaaaaaa atcttcgact gcaagaatct
    53761 ttaacaagta ttggaaacgg ttatatgggg atgcgcggga actttgaaga aacttactca
    53821 ggggaccacc atcaaggtac ttatttggcg ggtgtctggt atcccgataa gacacgtgtc
    53881 ggctggtgga aaaatggtta tccagaatac tttggaaaag tgatcaacgc cttgaacttt
    53941 atcgcgatag atatttcgat caacgatcag ctgatcgact tggcttcttt ggaaccagaa
    54001 gacttttact gggaattaga tatgaaaaat ggtgtattat ctcgtacatt cacaatcact
    54061 acttctacta ataaagtacg catttccttc gaacgttttc tgagcattgt caaaaaagaa
    54121 tcagcttata tctatttaac tatagaaatg cttgaaggta gcggaacagt caaactcgta
    54181 tcaaaattgg atggaaatgt tcaaaacgaa gacagcaact atgatgaaca tttttggaat
    54241 gagattgatc gtggaactat agaagaaatc ggctatttga cgactaaaac tattccaaac
    54301 aactttggga ttgaacaatt tactgttact gcagcaatgc gccatttcgt taatggaaaa
    54361 tcagtcatcc ctgactatca cgaagatact ttagcattac atggttgctt gtctgcagaa
    54421 ttatctcaag gtgagacatg gtcagtggaa aaagaaatta tcgttcttac tagtagagat
    54481 attccagaag cgcagcaagc agaaaaagct gtctcagaat taacgaacca gacaaacaat
    54541 tatatccaag caaaagaaga gcaaacagcg gcttgggcag aacgatggaa gcttgccgat
    54601 gtggtcatcg aaggagacga agaagcacaa caaggtattc gattcaattt gttccaacta
    54661 ttctctactt actacggcga agatgaacga ttgaatatcg gccccaaagg attcacagga
    54721 gaaaaatacg gcggagccac ttactgggat acagaagcgt atgctgtccc tctttatctt
    54781 gctttagcca aacctgaagt aactaagaat ctgctgaaat atcggcataa tcaactgccg
    54841 caagcaatcc acaacgctca gcaacaagga ttaaaaggcg cgctttatcc tatggtcacc
    54901 ttcactggtg tcgaatgcca taacgagtgg gaaatcacat tcgaggagat tcaccgaaat
    54961 ggcgcgattg cctatgcaat ctacaattat acgaattaca ccggcgatac ggaatatttg
    55021 aaaaatgaag gactggaagt tttagcagaa atcgcacgct tctgggcaga ccgtgtccat
    55081 ttttcaaaac gagcaaataa atacatgatc cacggtgtga ccggcccaaa cgaatatgag
    55141 aacaatatca ataacaattg gtatacaaat acgatcgcag cttgggtctt gcgttacacg
    55201 cgagaaaatt atttaactta tcaaaaagaa gctgctatca ctatcagcga agaagaacta
    55261 gccaaatgga aagatatcgc agagaacatg tacttcccag ttgacgaaga attaggaatc
    55321 tttgtccagc atgacacatt cttagataaa gatctcatgc ctgtatccga tattcccaca
    55381 agcgatcttc ctctgaatca gcattggtct tgggacaaaa tccttagatc ttgcttcatc
    55441 aagcaggcag atgtactaca ggggatttat ttcttcaatg atacttttac aaaagaagaa
    55501 aagcaaaaaa actttgattt ctatgaacca atgactgtcc atgagtcttc cctttcgcca
    55561 agtatccatt cgattttagc agcagaatta ggaatggaaa gcaaagctgt agaaatgtac
    55621 cagcgaacag ctcgcttgga tttggataat tacaataacg acacagaaga cggcttgcac
    55681 atcacttcca tgactggtag ctggttgtcg attgtccaag gatttgctca aatgaaaact
    55741 gcccatgatc agttgagatt cgcaccattt ctgccaaaaa cttggaaagc ttattctttc
    55801 catatcaatt atcgcggacg tttattgttc atctcagtca cagatgaaca agttactttc
    55861 gagttattag aaggttctcc tctagaaatt tacgtctatg aacagtctta ttctttagcg
    55921 gatcgtctag aaataacgct aagaaaggag gaagcaaatg tttaaagggg tattgtttga
    55981 tttagacggt gtcattaccg atactgctga attccattat cgtgcttggc gaaagcttgg
    56041 tcaggaaatc ggcattacga tcgaccacac attcaacgaa cagctaaaag gcgtcagccg
    56101 agaagactca ctctctttgc ttttagctta tggcggaaaa gaacactctt tttcaaagga
    56161 agaatttgca gaattagcga aaagaaaaaa cgattattat ttagagatga tccagacgat
    56221 cgaaccaaaa gatgtcttcc ccggggtcgt gcctttactg gattctttaa aagaagaaaa
    56281 aatcaagatt gctcttgctt ctgcaagtaa aaacgggcca ttcctacttg aaaaaatggg
    56341 tcttacacct tactttgatg ctattgccga cccggcagaa gcaacaaacg gaaaacctgc
    56401 gccagatatc ttcctccttg cagcaaaagc tgtcggacta aaagcagaag aatgtatcgg
    56461 gatcgaagac gcgcaagccg gtatccaagc catcctctca agtggcgctc aaccaattgg
    56521 cgtgggacgt gctgaagacc ttggagagga gatcccgctt gtccctgata cgacttttct
    56581 tacgatcgat tacttgaaag aaaagtggca tgaccatgga taatccagag atctccgtcg
    56641 taccagttcc agagactgaa ataaagaaaa tcacattgaa gaacgactac ttagaagtcg
    56701 ttcttctcaa ttacggtgcc cgcctgcacc aaatcttcgc accagacaaa gaaggaaaat
    56761 cagaaaatgt tttattgtct tacgatcgtt tcgaagatgt tctaacagac aaaagctttt
    56821 ttggagcaac tgtcggacct gtagccggaa ggatccggga tgcttcttgg ggtgagcata
    56881 gattggagaa aaactgcgga tcacaccata tccacggtgg cacgaaaggc tggtctttcc
    56941 aattttggga agtagaagtt ttcaaaactc catcatcaat aggtgtggtc ttttacttaa
    57001 aagatgaatt ttctacctat ccaggaccaa tcactgctac gatcacttac cgtttaacaa
    57061 aaaacgaatt agagatgaca acctctgcta gtagccttgt tgaaaccatc tgcaatccga
    57121 ccaaccatgc ctacttcaac ctttcaggaa atggaacaag agacatctat gaacatcagt
    57181 taacggtctt tttagatggt gtgcttgaat tagatcaaga aaaactgcct actggaaatt
    57241 ggacaaaaaa agaaaaactg ccaatcgact ttagaaaatc cccaacactg caagagatcc
    57301 ttgcatgtta tccagatgga ctagatgatg tatttctttt gcatcatcct aggctttcca
    57361 aaacttcctt acaattattc gagaagcatt ctggtcgcca aatgactata gcaacttcta
    57421 ataaaagtat ggtattattt tctacgactg gattcgaagc tgatttctct gtcaatggaa
    57481 aacagatgca ttcaaactac ggattagcaa tcgaaccgca agaaatccct gatatcgttc
    57541 atttccccaa atttggatcg atcaacctcc atcctggtca agaacggatc agtcagacga
    57601 tttatcggtt ctctgcacaa taacaagaga aaggtgtttt tatcatggct attaccgtaa
    57661 aagatgtggc gaaaaaagcc ggtgttgcca cctcgaccgt ctctcgtgtc atcaacgatc
    57721 atcctagtat ttccgagaca acaaagaaga aagtccgaaa aattatggat gaactaggtt
    57781 atgttcctaa catgactgcc cgcaatctag gaaaacgcat ttccagtgcc atcggcgtaa
    57841 tcttaccccc tcttgattca aaagagcgga tcggtaaccc attttatctt gagatcatgg
    57901 aagcaatcaa cgaagaagcg cggaattatg atatgactac agcaatcgct actgctaaaa
    57961 attttgaatt gctgttagag aacgtccagc gtatgcattt acaaaaacaa gtagatggtt
    58021 ttattctagt gtattcagat agtcaagata ctgtcattga ttatttatac caaaaccaca
    58081 tccctttcac tttgatcgga cagccttatc atcatgagaa cgaaatcgtt tatgtagata
    58141 atgataacca gcttctaggt aaacaagcaa cagaattttt gatccagaat ggacataaga
    58201 atatcctgtt tgtgacaaac acgactcatg aaagtttgta tttcgaacga tatttcggtt
    58261 atcaaaaagc aatgatgatg gctggtcttc ctgcgttatc cagtgtagta ctagagcatc
    58321 cggaggatta tctgtccttt gagaaaacgt tgaaagaaaa cgaagccaca gcattggtgg
    58381 tcattgacga tatcttcgcc cttcgcacga tgcagttggc gcaaatgtat ggctatgtca
    58441 ttccagagac actctccgtc attagcttca acaactcgat cttctcaaca ttaacacacc
    58501 cttaccttac aagtatcgat atcgatatct ccgaattagg acgtgtcgct atgcaaaagc
    58561 tgcgcgaatt gatccatgaa gaattgagca gtggcgtacg tttggtcatc cctcatcgct
    58621 tgatcaaaag agagacagtc gttcctttag aaaacccata ctaaatagct gactcactaa
    58681 atacgctgac catttatcct atggtcagct ttttatgtaa aagaagatac tacttctaac
    58741 acattgttta tctgtaaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
    58801 tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag ctgaaattca
    58861 caaaaattta aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct
    58921 gtcccagcct cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc
    58981 gcctaatatt gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa
    59041 cgcgctaact ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga
    59101 attgacggtc gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac
    59161 gtcatcaaaa gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc
    59221 aacgatcaga ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg
    59281 gtccatcttt tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa
    59341 cccacctaat gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc
    59401 gcctcgagaa agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat
    59461 caagatgtct gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag
    59521 agcagctgtc gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc
    59581 aaataaaata actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt
    59641 ttggatcatt tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt
    59701 ataaaaaatc aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg
    59761 ttcgaataga ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga
    59821 gcagttattc cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa
    59881 tccaagtaaa tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga
    59941 actgccgaca gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg
    60001 cacagaaaca agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca
    60061 tgaagcaact aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat
    60121 tcctttgatg atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa
    60181 caacaaacac atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat
    60241 cagtactcct aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat
    60301 atttttattc atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat
    60361 tcacttggca ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt
    60421 ttatttttct tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt
    60481 gatgatcaat ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact
    60541 caatgtcgct gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca
    60601 gctgactacc caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc
    60661 tggatgtttc ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata
    60721 aataacagtt gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc
    60781 ataaagcaaa gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac
    60841 gccgtatttg ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac
    60901 ttggatgatt gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat
    60961 aaataccatg aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc
    61021 aatcaagata ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc
    61081 tacaagcatc gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc
    61141 ttttgtataa ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat
    61201 cccaacaaaa ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc
    61261 agggaaagct tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt
    61321 gaaggctgcc aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca
    61381 tacaccgatg ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat
    61441 ccagtagata actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt
    61501 accaaatgtg tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa
    61561 catttgcacc cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt
    61621 tacccataac tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat
    61681 tgatatcgga ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc
    61741 gactgcggct aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat
    61801 aaaggtccca agacccattt tacttacttc agatttactc accgttgaca ctctccttga
    61861 ttttttggat aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag
    61921 cgaatatttg aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc
    61981 aaaacaaagg gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca
    62041 gaaacagtta gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt
    62101 gccaataatt ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt
    62161 tcagcatatt cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc
    62221 aatacagtta cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca
    62281 aattgtaatg ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac
    62341 tcattaccgt aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat
    62401 ttattcattg caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg
    62461 aaatctggat aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag
    62521 tcattcaaga agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca
    62581 tatcctgtga cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc
    62641 cctgcttttg aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt
    62701 tcaaatacat atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg
    62761 ataccaccgg cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct
    62821 gcttcttcga ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct
    62881 gtaaagacat tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg
    62941 tcttcgtcta agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag
    63001 aagtaaatac cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg
    63061 attgctcctt cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca
    63121 gctgctagac cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca
    63181 actggcactg gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat
    63241 gaatagtgtt tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta
    63301 cgtgctgagt gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg
    63361 atttcttctg ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt
    63421 gtcacttctt tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct
    63481 aagtttgcta aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat
    63541 tttgcaaatt ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag
    63601 gcaacgttat taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat
    63661 gtttcagagt tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta
    63721 cgcatccgcg ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa
    63781 ctagcactgc ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga
    63841 cgccagccta aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc
    63901 tcagctttgt ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc
    63961 atagtgaaac cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc
    64021 ttctattctg taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt
    64081 atccgaattg ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa
    64141 ctccctcgca aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa
    64201 ttggaatgtt gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa
    64261 ttactttggc caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga
    64321 attcatcgga tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta
    64381 tagctccgtt tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa
    64441 caagcagttc cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga
    64501 atccttgcgc gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg
    64561 ttattttttg cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg
    64621 ccaaacggcg ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt
    64681 cgatttcttc ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat
    64741 cttgattcaa ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa
    64801 cggcaccgcc tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg
    64861 ttaaaccgaa cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg
    64921 tgatatttcc ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca
    64981 gatgcaaaaa gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta
    65041 cttccaaacg gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac
    65101 cgtagtctct caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa
    65161 atgtgatatt tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt
    65221 tatgttgcac ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc
    65281 cgatcgttcc tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc
    65341 ttttcatcat cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa
    65401 cgccgcaata aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg
    65461 tttgctgatt aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct
    65521 ccgtgtagtt tttttatgaa agcaggaacc tgctagcata tgtaattaat tatttcttca
    65581 ggataggata ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg
    65641 gtaccaccca atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg
    65701 cgtggcaatc actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt
    65761 gcagcgacca ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt
    65821 cttgttatta ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa
    65881 ggatattttt tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt
    65941 ttattttcaa ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga
    66001 gaagtcactt tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa
    66061 aaaaatgaaa aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc
    66121 cacttctgct ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc
    66181 tacaaaaaaa gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac
    66241 agatgataag caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga
    66301 ccaagcgatt gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct
    66361 tcttcaaaca ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat
    66421 ctctcatgtc tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca
    66481 ggcacaagga tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca
    66541 actttatcaa aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca
    66601 aggaaatctt tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc
    66661 acaagatgat gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt
    66721 cgtatcaaat aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg
    66781 tgactttgac atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta
    66841 tcgaggcgca aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac
    66901 atcaaaatct caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa
    66961 agtggcggaa cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt
    67021 gaatatgact caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga
    67081 agttgcacct atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag
    67141 cgactggcag ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt
    67201 cgatattttc tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt
    67261 ctatcgaaat gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa
    67321 aaatcctatt cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga
    67381 atctgcttga ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag
    67441 tttcggcttt tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt
    67501 ccattctgct gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac
    67561 cattgcttga aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc
    67621 tttccgccct cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc
    67681 catcgcgtca gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata
    67741 aaattgtttg ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt
    67801 ttcttcaaat ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat
    67861 aaatgcaaaa aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt
    67921 cgctttactt acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat
    67981 gtctgtgttc atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct
    68041 cttttcaaaa ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata
    68101 gttcaaattt attctgaggt ttattataac tatctattga gaaagtatca agctagcaat
    68161 tccttgttta tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat
    68221 aaaaaaagtt ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt
    68281 atatattttt agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt
    68341 gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg
    68401 tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact
    68461 tttagggatt ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga
    68521 agatagttta tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg
    68581 aaatttgact ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc
    68641 tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa
    68701 ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga
    68761 tctctattta tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca
    68821 agaagaagtt gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct
    68881 cagtgaagtc gagcgactag cggataaagt cgtgatcata cgcgaaggaa cactcgatga
    68941 agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga
    69001 agcagagaat agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca
    69061 cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa
    69121 attaggtgtg aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca
    69181 ttatgaagga tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt
    69241 ttctcaaaac cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc
    69301 tttttctggt ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg
    69361 attatacgaa accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc
    69421 gaatgctgct gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag
    69481 tttgtttgct gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga
    69541 acttggtctg attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc
    69601 tgtcatcgct gaaagtattc tgatcaattt gcttttagca atatcgatca gctgtacgat
    69661 gatttttttc caagcggatt cgatcactgc agaaggtgct tggctttttg ggctatctgt
    69721 aggtagtatg gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag
    69781 ttcagccagt tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc
    69841 aataacagat gtagctgatt cctccctttc gatgtggaat cctttaggct ggtcttattt
    69901 gacctatcca tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct
    69961 aatcttgata atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat
    70021 ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt
    70081 attctatcta aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc
    70141 ggcatacggc tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa
    70201 acagatgttt atccaatctg gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt
    70261 agtcttgatt tgtctagctg ctattttacc aattgctagc atcaatcgtt tatttacaga
    70321 agaaacccac ggacgtatga atcagctgtt cgcaacaaaa atttctagga accaacttta
    70381 ttggaccaat atgttactag ctattctatt tgctatattg gcaatcctga ttagttctgg
    70441 tgcactaggg ctttctgctt tggcttcgat gaaggatgcg aaagacatgg atctattgat
    70501 gtttcttaaa gccgggttca atttcctgcc tgccgtttta gtgtttatcg gaattagtgg
    70561 ctttctattc ggatggcttc ctcgaatcgg taaacttgct tatatctact tagcttactc
    70621 ctttgcactc aattactttg gcggaatttt ggatttacca gaatggtttt ccaaaacagc
    70681 tgtccctagc tgggttccac ggatgccaaa cgattctttt gatctttccg tgttccttac
    70741 gttatcatgt atcagccttg cactgttcgt ccttggttct attggttata ggcgaagaga
    70801 cttactagaa ggataataaa aaagcatgta caatttcatg ctagcctcca aatctgggtc
    70861 aaaaatccaa catttggagg tcattttttt atttacaagt cttctaactg ctctttatca
    70921 atgatccaga cttctcgatt ctttcttttt acataaccat tctcctctaa taatttaaat
    70981 tttctagata aagtttcggg ggtcgtaccg ataaatgcag caagttcttt cattttcatt
    71041 ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt caagcagata gacagcgact
    71101 cgttcttcta ctttttccat catcaaaaaa gttgtctgct gctgtacttt tgttaatttc
    71161 tcaacgttga ttgctaaaag tttcagacta agggcgggat aatgcagtaa gagttccgag
    71221 aaatcttttt gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc aaataaagac
    71281 tcatttggaa cgcctagcaa ctggctctct ccctcatatc cgcccggctc tattactcgt
    71341 aaaagctgtt cctttccgct agcagataat tgatacactt tcatactgcc ggaggccacg
    71401 atcactaatt gaggttctcc attcggcgca agaatctgtt ctcctttttc cacatagtga
    71461 tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt gaacaaagga
    71521 acaaatgaaa cacataaatg atcatgactc ataatctctc ctccttctaa acagctattt
    71581 ctaataaaga aacataactt cccttattcc aactgataat tttcttttat catagcataa
    71641 acgtcaagcg aaccagatac cagcaaatag agtggatttc tttttgtccc ttgctttatt
    71701 ttccttcttt tgagacctta agtaattgat tcctatcaat tttttaaata tttacttttt
    71761 gaataatcaa aaacaaacct tcactcgcaa gccgagagta aggtttgttt ttcgcattgc
    71821 ttatttcgtg ttgtctattg aaatttttcc gcacctagct tttgatatag tggtgctagt
    71881 tcttgtaagg tttcagcggt tatttgctca acgtgttcga tttcttgcgg atagaagaat
    71941 agtttaccca tttgaaaggt tcggtctctc atcgcttttt tcaactcttc ttgtttcttt
    72001 gtatcggaca aatagtcaga caaagctaag tggtcgtcaa gttcatgttc tggttgcggc
    72061 catatgtaat aatccttgat atcgactgtt ttgctccact ctttcagtat cgcaagcaac
    72121 tggttatacg ctgcaatgct accatatcgt tcttcgcttt tataatgttg gtacatcaaa
    72181 tagatttggt attgtttttg attcaacaaa actgctagac aggcattctc gtcttgacga
    72241 gattcgcttc ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt
    72301 tcgattttag gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt ctggagctga
    72361 atctctttcc atttttgcca gtgttcttta tattctttct tgatttgttc aatcttatct
    72421 gttggatggc tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa caactgataa
    72481 tctgattttc caagttccat tgtttcgcct ctttccgtta tcttttgtat tctacctgaa
    72541 ttttgcgata tggacaaata gaggttgtga aaaagctgtt ctgcatcaag tcataagaac
    72601 agccaaacaa aaaactgcga tgagcatcac cgcagtttta ttgaacttaa ggttgattga
    72661 tcacatctaa aagcgttgaa aataaaatgg cactagaagc agctcctggg tcgatatgtc
    72721 cgattgcacg ctctcctaga taggaagctc gtcctttttt agctaccaag actttggtgt
    72781 gttcttttgc ctggtcaatg atttcttttg ttaaattccc tgccttcaat gcctctgtga
    72841 ctggcagcca aacatctaac atcgttttat cgtcagcttc tgcctttcct cggctttgaa
    72901 tacccgctgt tccctgctcg atcacttcgc ctaatttttc ttgagaatca atagaatcga
    72961 cctcttttgt cgctttcatc atgttcatga atgcagatcc gtataacgga ccagaagccc
    73021 caccgacttt cgatatcatc gccatagaga atactttgaa tgtatcgctg attgtttcag
    73081 gatgatcggc ttgaaaagct tcttcataag ctgaaacccc tcttcccatg ttatttccat
    73141 ggtctccatc gccaatcggg gtatctagat cactgagata ttgtttgttc tgattgattg
    73201 cctctgcaaa ttgcgtgagc cattcttgga tttcttgaac ggttagtttc atattatcac
    73261 ccttttatct tgagattttt taccatgaaa tcgtctgtac attgctttct aaagaatctt
    73321 tccattgtgt ctccgtcaag tcgatcagtg ttagagaaag accttgcata tctagtgagg
    73381 tcataaaatt accgactttt cggaaaacta cattgacgcc tctttcttcc aacagagcta
    73441 aaacatcatt catgaacacg aactgttcca tcaaaggcgt cccgcccatt ccgttgacta
    73501 gtacacctgc ttcttttggt aaattcgagt aatcatctaa aatcttagta acaagttcct
    73561 ttgccagtac tttagaaggc tgcatctttt ctcgacgata gcctggctca ccgtggatac
    73621 caacaccata ctcgatctca tcttctccta gttcgaatcc tggtttccct acttctggta
    73681 cagttgctgc tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc ttttctccca
    73741 gttctttcaa ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg attttgtgta
    73801 caaggacagt acctgcaact ccgcgttttc cagcagtata cgtactattt tcaacggcga
    73861 tatcatcatc tacgatcaca ctttctactt caatatcgtc catagcagct aattcctttg
    73921 ccatatcgaa gttcaatgca tcccctgtat aattcttaac gatcagcaaa actccttttc
    73981 cactatcggc ttccttaata gccgtctgaa tctgatctgg agttggagaa gtaaacacat
    74041 caccaagcac cgctgcactt aacatccctt cccctacaaa cccggcatgg gatggttcat
    74101 gtccgcttcc tccaccagaa accaaaccaa cttgttccgc tttctgattc ttggctacaa
    74161 cacgggaaga ttctacacga tgaacaagtt ctggatagct ttttactaat ccttccagca
    74221 tttcttcgac aatattgctt ggatcattca tgattttttt caatttattc ccatcctttc
    74281 tttccggcat tttgtgcttc tgttaagatt tgctgagtat catctgttat accggcagtg
    74341 atggctgctg caaaagctcc ttccactaaa ggcgcatcca ccagatgata acgttgctgc
    74401 tgctcttctt ccaacatgtc ttttgccagt tccgagttca ataccgcgct accgatatcc
    74461 gcaaaaacaa aataactagc tgcatcgctt gattcattga ctgcttctac gattttcatc
    74521 gggtctgatc cgatctcgcc atttattccg ccgagagaaa aaatttgcac ctcttcggat
    74581 ttttgcatct gctcaatcat ttctttaata ccatctgtta ctttttgact atgagaaata
    74641 agcaaaatac tttttttcac aagaatcttc ctttctttta catagttgct aacgattggt
    74701 ttcgctttca ttaatgaaca attcaagtat ataataaatt tctaattttg ggaaaatatg
    74761 tgtagtgctt cttgaagttt gttatacttt aattaaaaga gaggagtgat cgaatgatta
    74821 caacaacaac gaacagtgta gagggaaaag ccgtagtagc ttataaaggg atcgtttttg
    74881 gtgaggtcat tactgggatc catgcattta aagacttgga ggcggggttg cgaaatatct
    74941 ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa gaagcacttg
    75001 ccgaaatggc cgaacgtgca aaagcattag gagctgatgc gatcattggc gtaaaaatgg
    75061 actatgaagt attaggcagc gacaatggca tgctgatggt cacatgtagt ggaacagctg
    75121 tctccacgag ataagtaggg gacatctgtt ttaataacta aagagagatg ggacatgtag
    75181 tggcactcaa gaattagatt tttcagtcta acttcgaggt actctacctt cccatcctct
    75241 cttttttatt tctttttgcg gatgattccg taagctgctg cgactaacac acccgcccct
    75301 gctaaggaat aactgacaga agctgttttt tcatttgttt ttggcaaagt ctgacttgtt
    75361 gatttggatg gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga ggtcactgat
    75421 ttttctcctg ccgaatgcaa aatatctttt attcgttgga aactagctgc taagtcagct
    75481 ttttttgctt caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc gtggattgct
    75541 tgactggccg ctgtgatatc cgcttgagtt tgacttagct ttccagaagc tgtttgagca
    75601 acctgattta attgctgatt gaattctttt gcttgttgga tcttcccgtc aagatctgtt
    75661 tggacagctt ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg tgcacggtca
    75721 tttcccactt tatttttaag atcagctaaa tcttgatcga ttgctgcttg gtcttctttc
    75781 aatttgtttt ttgcagcttc taatgcttct tcgagttctt ttcttttatt ttccaagtct
    75841 tgcaaatgat cgatttggtc agaagaggta gaactattta ttgatgtatc ttctgaagat
    75901 gaaatcgttt ctcctgaatt atctgtcggt acattggaag tcatttctgt ttccgaggca
    75961 ccgttttcag caccagaaga gcttatggtt gttgaaggtt ctgtttgagc aggttcagaa
    76021 gtttccgcaa aagctgtctg tcccaataag aaaatactgg aaacagcaat tggccagatt
    76081 aacggcttca ttcttttttt catcactttt cccatcctta ctacttttta actaatttct
    76141 tattataagc aaaaatgttt ggaagaacca taaacacaac aggctgtttg tctaaaaaac
    76201 ggtgcatctt ttattttttt gtacgttccg ggcttttcca tgtcccgctt ccataaccta
    76261 aaatcgtttt gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc actatccaga
    76321 acaaaagcat atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt cgatcatcta
    76381 caatcagcga agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag atgaacacaa
    76441 aactgaatgt aaaagtaata taaatctgtt cgtagtttcc ttgatataca taatgcccga
    76501 ttaatccagc aaaaagaact acggaaatac aaaagaatat tgaccagaca atactcaatg
    76561 tttgatcaat gaagatgatc gttcttccaa tatgtttaaa aggatggagc atcacttttt
    76621 tgaaattcgt cagccagact tctgttccac cttttgccca acgttttctg tgtcgatata
    76681 gcatcttcaa cgtcacaggt acttccatga aaaagataat ctcaggagca aaaacagata
    76741 accagtcatt taactgatga tcccaagcaa tactgatgtc ttctgtcgct cgatcttgtc
    76801 tgaatccgcc cacatcgatc aaggcttctt ttcgatacat cgtatttgct ccactgtacg
    76861 cataaagacc accgaaaaca gctgactgtg ttcgtttaat gatgcccacg atacttgaga
    76921 attcaaccgt ttgagatttt gcaatcaatt tggtacgatt ttggacatcc atatttgcag
    76981 tgaccgcagc aatatgtctt gcaccagggc gaatgaaata attcacatac ctaatcagtg
    77041 catctggctc cggtaccgtg tctgcatcat tgcttaaaat caatttccct tttgcaaagg
    77101 caagtccgat attaaacgcg tgggcttttc ctttattttt ttcgatccga accacacgaa
    77161 gattagcgta ttttttcatc agtctagcta atatttctgg tgtttggtcc gttgaaccat
    77221 cgtctgtcac taatacctca tagttgtgat aattcaattt cgtcatcaaa tactcgattg
    77281 tatattcgat cacaatttct tcattatggg ctggcaccat gatcgtgatg aacggttcga
    77341 cactcaaggg aacatctacc cattcttttt gcttatgttt gtataaaaac acataacacc
    77401 agacaccaat aaaccataca aatccgccta aaatcggata agaaacgaga aaaaaataca
    77461 taatagacaa acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag
    77521 ttccacccca ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc
    77581 ggagacagaa taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata
    77641 cacttcttca agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt
    77701 atttactttt ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag
    77761 gaaactgaaa aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga
    77821 taaaaaagcg tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac
    77881 ccatataaaa ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat
    77941 ctttaaccag taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc
    78001 ttctcttgtt acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt
    78061 accatacatt tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac
    78121 aaaacatttc tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta
    78181 ttttttatct cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga
    78241 tatcgccgtg tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata
    78301 taagattaca tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt
    78361 ttctaatcca tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt
    78421 ttgggtgata actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt
    78481 caacaattct tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat
    78541 cgatacgatg tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc
    78601 tccgctagaa cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc
    78661 gaaacaacgc ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc
    78721 acctactaaa gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag
    78781 ggtataatct tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc
    78841 aacaagtaaa tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg
    78901 ccaagcataa gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc
    78961 cacccatgcc atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt
    79021 ccttgcataa gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc
    79081 caactgccta tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg
    79141 tttcaaaaga tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc
    79201 gatcaattta ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag
    79261 gactgttttt ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg
    79321 atctgattgt tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga
    79381 tacattttct cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga
    79441 aagctgtgaa accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg
    79501 taggacattc gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc
    79561 ggatgtcatc gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga
    79621 ggttttacca cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat
    79681 gattgtgttt tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc
    79741 ttggcctaag acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt
    79801 gaccaaacga tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt
    79861 attttgacga accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat
    79921 tggtgaacgc caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc
    79981 gatataaaag gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta
    80041 gggacttttc aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat
    80101 attcatctga tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc
    80161 taagtaactg tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc
    80221 ttctattttt gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg
    80281 atacacgttc ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct
    80341 cctctttttt taaagtggtg gaaccgatta gtttacctct tttaagagtc gaatgcaagc
    80401 tttttaacga gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata
    80461 aagaaaatgc accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg
    80521 ctagatagct acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt
    80581 catttaatcg gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct
    80641 tttccaattc ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg
    80701 gcaattcatg ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc
    80761 ctagcatgtt tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt
    80821 ccggtaaatc gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt
    80881 tatccacacc ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga
    80941 agatcaactt cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg
    81001 atacatgaat gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg
    81061 tggatgcggc agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc
    81121 ttcgagaaat tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag
    81181 caagagaaat aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca
    81241 ccgcataaat atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta
    81301 tgcctaattc gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata
    81361 aaataactaa atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt
    81421 cttcaaatcc agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt
    81481 tttctataaa agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga
    81541 tcaccatgac ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc
    81601 ctgcaccaac gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga
    81661 tatctggtac atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg
    81721 gaacaactgg ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt
    81781 gaccctcatg tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat
    81841 ttggacgcac atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat
    81901 tttcccgttc actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa
    81961 catacggtcc tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg
    82021 tagcagcaga cattccacct gctacacctc cgacaataac gattttcatc ttatttccct
    82081 cctactgttc gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca
    82141 gatttcttta attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag
    82201 acggtctttt ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct
    82261 ccttttatat gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt
    82321 tccaaaagct tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa
    82381 ttcattttct gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga
    82441 gctttattaa tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc
    82501 ataaaatacc gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag
    82561 attgataata aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc
    82621 caaaaaatga tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt
    82681 cgttctagta ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt
    82741 gagaaacctg atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg
    82801 attattaaaa agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga
    82861 aaaatggtca gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac
    82921 gattgtcgta tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga
    82981 attcttagat ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca
    83041 tatttcagaa agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt
    83101 agttgaactg caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac
    83161 agaagtgatc agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac
    83221 cgtgaatcaa aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca gccaaacaaa
    83281 aatagcttct catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
    83341 accgtttaag gaagaggttg tgaaatcagc ggtttgacct aagtattaga gaaaaaaacg
    83401 ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
    83461 acaccgttta aggaagagat tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt
    83521 tgtgaaaatg gatttctaca tttttgcata aatttggctt aataccgaga aactggcttt
    83581 tgaataccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagaaac
    83641 aaacggaaaa atagctgctc atattttgcc gttttttgag ctaataccga aggtcagctg
    83701 attgaacacc gtttaccaag aggttgtgag cctaccattt aactccgaat cacaagaaat
    83761 aatttcaaaa acaacttctc ctgtttttgg ttcggttggc ttattatcga gaagtagcta
    83821 ctgggagtct atttatcaaa aaaaggaatg cgacataggt cacacccctt ttttattttc
    83881 tttgaaacat ctttcggtac tcgacagcat acaagacaaa agattgtaaa agaccgtaag
    83941 tgatgacggc aaaaatgact ctcacccaca gcgttggtaa ttctgggaaa agcagcaaaa
    84001 ggatcatgcc aacatagatg acagcagtcg ctactttgcc ataccacttc gctccactta
    84061 atttgatatt tttagatagc ccgattaatc cagctacaaa cataaagcct tcttttatca
    84121 agaatagacc aaacaacagc caaagccacg gtcgatacca tgccatagac aaaatcagaa
    84181 aaagttgtgt caatttatct gcaaacggat caagcacctt ccctaagtct gttacctgat
    84241 tatatctccg agcaatgacc ccgtctagat aatcagacaa acctgagaca acaatgaccg
    84301 ctcctgctat ataaaatgac tgtctcagca caaaataaag ataaatcggc agcaaaaaaa
    84361 tacggaatat cgataaaaga ttcggaatcg tttgtatttc tttacgccag tctgtgctta
    84421 atttcatttt atcgccctac ctcctcagca ctcttttctt tttattttag cttacacggg
    84481 gaataaaacc aatgatttca ctgtttttat ttttctttac agacactgat attatgagct
    84541 aatttgagat aatcacttgc tgttttcatc gtttactctt ctttcttcct gtcgtttgcg
    84601 catttctttt actagtactt tatgagcttt ttttgatcct cgctctaggt cacgctgccg
    84661 tttacgtgtt ttataatcga taaacagact gtctaaatcg taacctgtcg ggtacagatc
    84721 ttctgctgaa cgcaccagac gaacacgctg acgaggcacg tggattattt ttttgtctaa
    84781 atatacatca atcgtatctt cacctatatc ttcataaact aacgcttctt tttggtattc
    84841 attgacaaat atccgatcac cttttgcaaa gagctgacgt tcttttttat tctccttcct
    84901 atttgtaact gctgtcttag gaagaaattc ttttttctgt gtccgatagg ctttttccgc
    84961 caagtaacgc tctgcttgat ggatcaattt catcgacata tccattttat gagcaatcca
    85021 caaagcttgg ctttctcctg tttcttccaa cccgcaactg atatttagga cgaagtgctt
    85081 cccgatcgaa agccatagca gccggcacaa aatcttcatg atctccggcg aatttcttga
    85141 tttctccata atgggtcgtg gcaatgatca atgccccttg ctcatacata gattccatga
    85201 ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc actgccaatc tcatctaaaa
    85261 gtaccaacgt attccttttt atctttttca aaatagcagc gatgttttgc atatgaccag
    85321 aaaatgtact caacgcattt tctaaatttt ggtggtcacc gacatcgacg aaaatctcat
    85381 caaagacagc aagctctgtc ccttcttttg ccggaacttg cataccgaac atcgccatca
    85441 aagtcaataa accgactgtc ttcaacacga ctgttttgcc tccggcattt gctccggtga
    85501 taatcaagcc tcgatagtct ttaccgattt caacatccaa tggtaccgca tgctcaggta
    85561 aaaatggatg tctgccttgc ttgataatga tatgttccga tttattgatt ctaggtgtgc
    85621 ttccattgat ttctcggcta aattttgccc gagcaaagat gatatctaac accgtgatcg
    85681 tttcgatcaa taaatcgatc acttcttcgt tttctgcgat agcaccagtc aatgccgcca
    85741 ggacttgata ttcttcactg atttcttcag cttttaacaa ctgatagtgt tcattcaatt
    85801 tagaaatcac agttggttca ataaaaaccg tcgttctttt atttgattca tcgataatcg
    85861 ttccatctat tttattttta tagctggctt tgataggaat cgtatagtat tcgccttttt
    85921 gaacaatcat tgcttcttgg atcatttctt tgtttttcgg atgacgcaag aatttcagta
    85981 attttgactg gatttctttt tcgattagtt gtaattgttt gcgtacttta cgcaaattac
    86041 gtgacgcgtc gtcactgact ttattatttt ttattttctg gtcaatcaac tcttctacat
    86101 ttatcaaatc aggaagatgt ttgctataag caaacaacaa gggtgtctgg tattgattct
    86161 tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa atccgcatat tcgatcaaat
    86221 cggttggctg cagaatcaac ccttttttga cttgttctgt cagtgcatcg atcctcggca
    86281 accccataaa tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct cttgtttctt
    86341 cttgacgatc caagacagtt tgtaggtttg tcgagaccgt catatcgctg attcgttttt
    86401 tgctatagtt tccaattgct cttgcttgga tttccttttt gatccgatca aattgtaatt
    86461 gactaatcat ttcgttgtcc atattctctc cttttcttga agagatacct gtcccaaaat
    86521 aaataagggc ctagagataa ataaaaggat agaacacagc ttaaaaaagt catgtcctat
    86581 ccttattaga atacaaagat attgttttcc tgacaggtac cgtttcataa gcacaccaaa
    86641 taaactttgt ttattggcta ttcgacttac tgattagata ccagctacac tcacaaaaca
    86701 taccccattc ttctaggtct tatttatttt ccacttaact atagcaagac acattttggt
    86761 tgtcaaactt tagtatgttt tatagattta caccattaat gaatagacac ctaccgcgat
    86821 aattgctccg atcattggac ctgcgattgg tacccatgaa tatgcccaat ctgaagttcc
    86881 tttattagct attggcaata ccgcatgtgc taatcgagga ccaaagtccc gagcagggtt
    86941 gattgcatat cctgttggcc ctcctaatga aagaccgatg gctaagatca atccacctac
    87001 agcgaatacg ttcgttccag gtgcaaattc gttttgtcca aaagctagca acccaagaac
    87061 taatacaaat gtaccaatta attctgtgat cacatttgct ggataattcc gtatagcagg
    87121 acctgttgca aacgttccta aaatcgcgct ttcatctttc gttgcattcc aatgtggcaa
    87181 ataagaaagc caaacaagaa tggcccctgc aaaagctcct aaaacttgcg caatgatgaa
    87241 aggaacgaca agattccaac taattgcccc tgtcatcgcc atagctagag agactgcagg
    87301 attcaaatga gctgggccca tgaatccaga aatatagaca gccattgtta cagcgagtcc
    87361 ccaaccaaat gcgatgacaa tccagccaga agcctgtgct ttacttttgt ttaaattcac
    87421 agccgcacac acaccatctc caagcaaaac taggatcagt gtcccaataa actcccccat
    87481 gatttgagtc atatcactgt tcatttattt ctcccccttt agttcccgca gatctgattc
    87541 tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc tcctcttctg tccattcaaa
    87601 atatccagca attgcatcga tcacaggttg cttgatctca tcaagctgat ctcgttcaaa
    87661 caacagatga tttgtacgac gaatgagata atcgcctggg gccaaaacca tttcttcttc
    87721 taaaccataa cgtaaccgag ctgactcagc gagactcaaa ccaggataag gtgccatttc
    87781 ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga tataagtagc
    87841 atcttcttcc tctaaaccgg ctgctactcc ttcttttgct aattctgtca ctgtttcttc
    87901 taatttagtt ggatcgaagt ttccacctga aatctgatat tttttagagt cgatttcttt
    87961 cgtctctatt ccgtattctt cttttaataa ttggcgtatc aatcgcaaag ctccttctgc
    88021 catcttacgg taatcggtga ttttcccacc agataaagtc agcaaaccat ctggttctct
    88081 ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt tcatcacggc tgttttccaa
    88141 atggttcaac acgtcttcta cttcagcacg agaaactttg ttttccttat attcacttac
    88201 tgtatcgaca actttattga agctcttgtc tgaaatcgat ccattatctc caccattgta
    88261 atcagaacca gaattaccaa tcaacagtgg acgaagtcct gcccagcttg cttcgatatc
    88321 attcaatgtg atatttgctt ctggatagcg atgattgatc acatccaata gataatctac
    88381 atcttcttgt gtgactttcg gatccgtaaa atctccttgg taatccgtat ctgtcgtacc
    88441 aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc cgcccatctt gctttcctgt
    88501 atcgaagtat gtgggttgcg gtacaggcag ttttttcgca tctacaacta aatggatccc
    88561 tttagttgga cgcattttag gagagactgg acgcgtaaaa ttcaagtttc gtactttatc
    88621 tacccaagga ccactcgtat tgatcactaa ttttgctttg atttcgatca cttcatctgt
    88681 cagcaggtca cgagctttga cgccaacgat ttgatcgcca tcatataaaa agccaaccgc
    88741 tttcatttta cttactagat aagccccatc ttcggcagcc tttttgatat tatcaatcac
    88801 taaacgggca tcattgttgc ggaaatctag atagacaccc gcgcctttta gcccttcttt
    88861 tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc agcgtataat tctcatattt
    88921 ggttcctgtc acatttgcca gtttgtcata aaggtccatt gctactttga ccgagaacat
    88981 attgaaggtt gttgctcctt cgccctcata gattggcaaa agcattggat ctggttttgg
    89041 gatgtgtggg gcaatacttt gtacgactgc acgttcacca actgtgtcag ctactacttc
    89101 cacatcaaat gtcttcagat aacgaatacc gccatgaaca agtttggtcg agcgagagga
    89161 agtcccttct gcaaaatcct gcatttcgat caatcctgtt ttgattcctg atgctgctga
    89221 ctggactgcc acaccggcac cagtgattcc gccaccgatg atcaacagat ccaattcttc
    89281 ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat ggatttcctc
    89341 cttattcaaa tgcttgggta gcttttactg cttttttcca accgttgtaa agtttttctc
    89401 ggcgctcttc ctccatttga acttcaaata gcttgccagc ttcataaaat tcgcggatct
    89461 cgtctgtatc tttccaataa ccgacagcta atcctgctaa aaatgctgcc cctagcgccg
    89521 ttgtttccaa gttttctgct cgttggatcg gtacattcaa gatatctgtt tggaattgca
    89581 tcagatattc attgtttgct gcaccgccat ctactttcaa tacaggaatc ttgattccag
    89641 tgtcatcttg catcgtatcg atgatatctc tcacttgata agcaatcgac tgtaaagtag
    89701 ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc cctcgtgctg
    89761 cctgatccca ataaggagca cctagcccaa cgaatgcagg aacaacataa acctcatctt
    89821 cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac atttgaagtc
    89881 catctctcag ccattgaatc gaagaaccag ccacaaaaat acttccttct aaagcatagt
    89941 aaacctttcc atttatgccg taaccaattg tcgtcaataa attatttttg gataattgcg
    90001 gtttttcacc ggtattcatg acaataaatg aacctgtacc ataagtattc ttgaccatgc
    90061 ctggttcgaa tgccatttgt ccgaataatg cggcttgctg gtctcctgcc atccctgaaa
    90121 ttggtgcttc gcttccataa aaatgataac cttgcgtata accataaact tctgagttag
    90181 aagtgacttt tggtaacatc actcgaggaa tatttagtaa atctaaaatc tcctgatccc
    90241 aatctagctg atggatattg aacaacatcg tccgactggc attcgagtaa tcagtaacat
    90301 gtgcttgtcc atcggtcagt ttccatacta accaagaatc gatcgttcca aacatcaatt
    90361 cgcctttttc agctcgttct tgcgcgcctt ctacatgatc aagaatccag cgtatcttcg
    90421 ttgctgagaa ataggcatcg atcactaacc ctgttttttc gtggaaaaat tcttgatagc
    90481 cttcattttt taagttatct gcaattccag ctgactgtct ggattgccaa acaactgcat
    90541 gatagattgg acgtcctgta tctttttccc atattactgt tgtttcacgc tggttcgtta
    90601 tgccaatccc agcaatctga tttggtttga tccctgattc gatgaacgct ccggcaataa
    90661 ctgactgtac agaattccag atttcatttg catcatgttc cacccaactt tcttgcggga
    90721 aatattgcgt gaactctttt tgagaactgc cgatgtgtct tcctttcttg tcaaagataa
    90781 ttgctcgtga gctagttgta ccttggtcaa tcgccatgat atacgttgat tctgtcattg
    90841 tgattccctc cattatatgc aaacgctttc tttaactact cttatcatac tactgtttta
    90901 aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt ttgacagttt tttattatga
    90961 agctattttt taatcaattc ttttcttttt tcagcgtctc tattcttctt ttcaatcgaa
    91021 taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt ttgtttctct
    91081 ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca atctcaatac
    91141 cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga taaatcggca
    91201 gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg atcatcaaaa
    91261 gaacattggc atactggatc atcaggtaac taagctcact atcttcagct cttttcctcg
    91321 gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag gaatgcccga
    91381 gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc tttccaacaa
    91441 agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt tcttctttga
    91501 ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa gtatctaaaa
    91561 cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt tcttcgtcta
    91621 ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca ttcagttctg
    91681 ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa agccggtccg
    91741 actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca ttcaatagtc
    91801 tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc gtaaacgttg
    91861 tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta ttttgcaaaa
    91921 aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa tatcgtatct
    91981 gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc tctaagagct
    92041 ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac gctgtcgcta
    92101 atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta aacttcaagc
    92161 tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc aacacaagca
    92221 gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg ccaatcaatt
    92281 caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc tctctcaaac
    92341 cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg atctgtaact
    92401 gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac ctttgaccac
    92461 accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc taactgttca
    92521 aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa catctctttt
    92581 ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa tttgagttta
    92641 actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc aattttttgg
    92701 ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata gccaaaccac
    92761 tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact aattgtcttg
    92821 ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat tttttcattt
    92881 gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt ttgagacgtt
    92941 tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg ttcttcccac
    93001 atctgattgt cgggacgaga atgattagcc agcaaaacaa gatcatagtt ctcctttctt
    93061 ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg ttcttctaaa
    93121 aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat ttcaggatct
    93181 agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt ttttcttgat
    93241 gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc aattttcttt
    93301 gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac tgggtgaata
    93361 agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc tagaatcaaa
    93421 cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt caatcctatt
    93481 actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc tttcgtgatc
    93541 caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa cccgctggga
    93601 atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg catcaattgg
    93661 cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac aatgttcatc
    93721 tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa aacacagtat
    93781 tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac cctctccatc
    93841 ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg aagagtatca
    93901 tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc gccttttacg
    93961 tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc gtgtgtaatg
    94021 taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa caactgcttc
    94081 gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat caatttgaac
    94141 cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca aagcatcttc
    94201 tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat tactgaaccc
    94261 agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg gcaacaatcc
    94321 tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt gtgaagcgac
    94381 tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac cagcataata
    94441 aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc ccaaggcccg
    94501 atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat tcaaaacact
    94561 ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat cgatcccatt
    94621 ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg taggacgctc
    94681 gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt tcccatcctc
    94741 tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa tgggcttggc
    94801 aaataaagct ctttatatgc tttttcaaaa ggtttcgtta caaattcatc ctcacggttt
    94861 tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat tgctgcaagt
    94921 tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc gacaccaact
    94981 aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt atcgttgtat
    95041 ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata atctagtttt
    95101 gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc tggtgtttca
    95161 tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata ttgttcaacc
    95221 gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc caagctaccg
    95281 ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat caaatacgta
    95341 tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg tgtggctttg
    95401 ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg tgtttccgca
    95461 tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga taaagttgct
    95521 cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc ttctccttgg
    95581 attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat gctgagttca
    95641 cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac agcaccaaga
    95701 cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc attgaaaaag
    95761 ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac tcctgaaggt
    95821 aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg ctctgattaa
    95881 cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa ctaatttgct
    95941 taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct gggctaattc
    96001 tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt cccagctacc
    96061 atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg acaagcgacc
    96121 ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac gagcaatcgt
    96181 tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat ctttttcttt
    96241 taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa acgcttcatc
    96301 agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac attcatctaa
    96361 cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat catagtatga
    96421 tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta gatttgaaaa
    96481 aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc ttctggaaca
    96541 ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa aattttgtga
    96601 aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg gcttttgaac
    96661 accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa
    96721 caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc agctgattgt
    96781 acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt
    96841 caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga gttggcagga
    96901 gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag tcccttattt
    96961 ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt
    97021 ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca tagatatatc
    97081 cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag ccccacgacg
    97141 tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca atatgctgct
    97201 tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact ttgtctatag
    97261 ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat aattgattca
    97321 aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat ggaagatacg
    97381 gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt tctggatgag
    97441 cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg taggctgcta
    97501 agatttcttc atcacctact tctattttta agttgacacc tttttcttca aacattcgtt
    97561 gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa gaagttcttt
    97621 gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca agaagctgat
    97681 agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct tttgtagcta
    97741 aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc aaatggggat
    97801 ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag ttgatctcat
    97861 tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt tcagcgaaac
    97921 gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta accaaatata
    97981 acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt agacattcgt
    98041 caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca tcaccatgag
    98101 gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt tcagcaaata
    98161 aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta ggataataat
    98221 acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata tgccattgat
    98281 ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac acagataatc
    98341 ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct ccgccccata
    98401 aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt ttcttgtttg
    98461 attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg taacaataac
    98521 cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt ccatcaataa
    98581 atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt tgccatttaa
    98641 attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac tgactacccc
    98701 aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacagaac tgtagatttt
    98761 tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa atgttggatc
    98821 gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca ctatatcctt
    98881 tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca tacaggaact
    98941 gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa tataaatcgg
    99001 gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg caccagctac
    99061 tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat atttaagagt
    99121 cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg cagataaaga
    99181 taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg
    99241 cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga aagaaagatt
    99301 gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca cttgccaaaa
    99361 tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta aatatccagc
    99421 tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaaa taattgggac
    99481 catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt gcaagctttt
    99541 gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg gaataaccgt
    99601 acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta tgctatcact
    99661 atatgcactg acaatcgtag gataaacgag agctgctgca ataaccatag cgatgaaacg
    99721 atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga aatagaaaaa
    99781 tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta ataaattcaa
    99841 actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga cgcctaaagt
    99901 cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt ttttctcagt
    99961 tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata atacgtcata
   100021 cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt cgactacagt
   100081 gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat cttttagggt
   100141 aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc ctccgacaaa
   100201 tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct tgttaattaa
   100261 tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt tctccattca
   100321 attagtaccc cttctaatag cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc
   100381 gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat agtcaaagga
   100441 gacaaaaaaa acacgcttga tcttcctttg ctgatgtata agtggtatct caagctgata
   100501 ctctgtgatt tttgtttcta cttggctttt gataagacag cacaacaaag tctcggcatt
   100561 taacggaagc ataactaggt cagaactagg agagattagt tgctgaatgg aatctatctt
   100621 tttatggatt tttatttgat tttcttcttt gtagtaatga tcaagcagtt ttgttttctt
   100681 tttttcttca actatggtca gcttttcacg caaaatctga taaatctccc aagattcaaa
   100741 ctcatctaga acactaggta gataaatcaa atcatcccac tcaaaaataa gctcttgctc
   100801 agtagtcaaa attgcatcat attccgttat tttgtttgat tgatttttga aaagatacac
   100861 tggttctatc cggcattctt tgatccacat gttcatactt tgttgaatct gtttttttag
   100921 tgtattcaaa atactaattg gctggttact aacaatcagt atctcccctt gttttcgaaa
   100981 aggattcaac gcttcggcaa gatacaaaac aaaataagcg ctttcagcta aattgggacg
   101041 taattgaaaa tgtattggga agaaacgccg aattaaataa atttctaaag gataacggga
   101101 gatgatcttt tgtacaaaat gattcatggt attatgtccg gcatccatcc tcgttttcat
   101161 ttgtttgata tgggttagca aaaattctgt ttcatggaac aataacgaaa caggaagatc
   101221 taaaaaacga ataatcgttt gctcaaatgc tcttagattc ttctctattt ctttgttttt
   101281 ttcatcatta atcatcaaat aatttaactc taatagtcga ttttcaattg cttgtttttc
   101341 ttgattggaa aaagaatagg gtagttctga tgataacttt tttgtgaggg tctctaccat
   101401 cggaataaat aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt
   101461 caaaacgaga taccgagaaa atcgtatgaa gtcctctcct gaaacaatat aattatatga
   101521 tataagaacc tcttttaatg tcttttggac agtcggcatg atattggaaa aaattgtact
   101581 gatttgttga ggaaaccttg cgtgttggat caccgtttct gttccaacaa gagataagaa
   101641 aattcttttt gaattttctt cagcaatgat ttttagtcct tttgtcgacg aaacttccag
   101701 ttctaattta ggattgcgct cgatccaacg caaaatcgtt tttatttgta atgatacggc
   101761 tgtttttgaa agaaagaaaa tgtctgctat cttttgcata ctgacatacc ctttttgaaa
   101821 caataagtac gtcacaatag ttgaagctcg gaaatccatg ctggaataga ttttatgttt
   101881 cgtcacctct tgtatgattt gctgtttagt ttcagattca attgattcta aaaaataacc
   101941 tgaacgttta tttgactgga taatgatttt cttctcgtag atagcaagca tatttttgat
   102001 ttcagtttgg atcgttttct tgttggtacc taaagcttca gctaattctt cgcttgttat
   102061 ccaatgattc ttatttctta atactacaag aatttggagt tgtcgtttat ttaagtccat
   102121 ttgcttacac ctcctctttg ttataaacat acaggaaaaa cctctcaaat gacaatagtc
   102181 acttgcagag gtttaaaaat catttgaaga aagatctata ttcaaggttt ttaccattag
   102241 actggataat ttgtttataa tattcaaaac tatctttttt caatctccta aagcttccgc
   102301 tcccatcatt ttgtctatcc acataaataa agccatatcg cttcctcatt tcccctgttc
   102361 ctgccgatac gatatcgatt ggtccccacc ataagtaacc aatgatttgg caaccatctt
   102421 ttattgcctc agcaatttcc tgaagatgta tttttagata ctcacaacgt tttggatcgt
   102481 ggattcgatt caattcatct ggtgcttcat ctagtccaat gccattttca actatgaata
   102541 atggtaattc ataacggtct gtcaattcat tcatgacata acgaaaccct agaggatcaa
   102601 ttggccaact ccaaggttcg ggagattttt ccttcaaaaa gggattatcg aaaccatttg
   102661 ctccgccagt atctacgcgc atttctgctt cttttttata gacagcgcta cgataataac
   102721 taaatgcaat ataatctgcc gtgttagctt gcaagcactg ttcgtctcca tctaacatta
   102781 ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata agctggataa tgccctcggc
   102841 acatgacatc ggagaaaaac catgaattgc gtttaaattg cagtgaacca aatacattct
   102901 ctggatcaca gtcatatgca taagtcgcta atgcagaaaa gctacacata atacccatct
   102961 tagcatttgg atcgattttc tttaataatt gagtagcacg ggcgttcgct agaaacatat
   103021 gatgacaggc ttgataaatc actttttttg ttagtgtagc aatgggttca agtttatctc
   103081 tagcagggaa tgtaaccaat ccagcagctg cataaggcat acgaaaagca ttattgatct
   103141 cattaaaggt catccaatat ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat
   103201 aaaattcaaa aaagtcgatt aattttcgat tttgccaccc cccatactct cctaccaatg
   103261 ctaagggggt ttcataatga ctaagagtaa ttactggctc gatatcatat cgatttaatg
   103321 tattgattag tctgtcataa aatacgagac cagcctcatt tggcttctgt tcgtctcctc
   103381 ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct aactccttca
   103441 gtaaacgaat atcctcttca aatcgatgat aaaaatcaat cgcttcatga gaaagataat
   103501 ggagtgattc gtctaaagca atctcccaaa tctttttttc ttcattccac tgaatactag
   103561 gatgctggtt catgatcccg ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca
   103621 accatgcacc ttcacattga ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa
   103681 atatttcctg atccataact atctctcctc gctatcaaca atttcttact tgaagtatag
   103741 ctcgattcat caaaacaaga tattctaact atttcggcag aagagaagaa aaaaattgaa
   103801 tgctactctt catttttaat atttatatat aacaaataca taagacgatc cccgaaagaa
   103861 aacactcgtt aaccatctaa gcaaacgatt tattttactt ccaacacaaa tgcttcataa
   103921 ggtcgtaaat caactttgcc gtctggtaac tgtttcagag gataatttga cacaataacc
   103981 cttaaagctt cacgtggaat agtgaacgtc tgcttttcac tggacatatt acaaataacg
   104041 agccattgct ggtcttcata tatgcgagaa taggcaaaga catgctcagg cgtcttttcc
   104101 actaattcat agtctcccca gacgatgatg tcatgttttc tacgcaaatc gatcaatttc
   104161 ttgtacatat aaaaaacaga atcttgatct gctaacgctt cttctgcatt gatttcttta
   104221 taatttggat tgactcgaag ccatggaatt ccagtagtaa acccagcatg ttcacgggcg
   104281 ttccattgca taggcgtcct agcattgtct cttcctttcg ccttgattga ttccaagatt
   104341 tcctcttttg taaatccaga agaaatcctt tcgttataca tattgattgt ctcaatatct
   104401 tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc tccttgatag
   104461 atataagggg tgcctttcat catatggagc aagatagcaa acatcttggc actttctttt
   104521 cgatacacat cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg attattccaa
   104581 aataaactat tccatccttc atcacctaaa gatgtttgcc atttggataa tacttttttc
   104641 agttcagaga tactcaaggg tttcaaatcc catttgtctt tgccttcttg ctgatctaat
   104701 cctatgtgtt cgaattggaa gaccatggat aattcatttc ttttaggatt ggaataaagt
   104761 ttagcgattt cggtagttgc tccccatgtt tcgccaacag tcatcagatt tttgtcacca
   104821 aaagttgctt gattcatttc ctgaagatac tcatgcagtt taggtccatt acttgtgatt
   104881 tctttatccg gttctttgcc aatcatatcg atcacgtcca tacgaaagcc cccaatacct
   104941 ttatcgatcc agaaattcat catttgataa atctcttggc gaacctgttt atttttccaa
   105001 ttcaaatcag gttgacgctt gctatacaag tgcagaaaat attgccccgt tttttcatcc
   105061 aactgccaag cagaaccact gaatattgag cgcaatctat taggctcttt tccattgacc
   105121 ggatcacgcc aaatataata gtcccgataa ggattttctt tatttttacg ggcctcaatg
   105181 aaccacggat gttcatcaga agtatgattc accaccagat ccatgatgat acggatattt
   105241 cttttctttg cttcttcaat cagttcttcc atttcttcca tcgtaccgaa ttctgccatg
   105301 attgcttgat aattactgat atcataccca ttgtcatcat tcggagactc gtatactgga
   105361 gaaagccaaa ttgcatcaat acctaaatac gccagataat ccaacctgga aataatcccc
   105421 cgcaaatctc caataccatc ttgatttgaa tcttgaaaac ttcttggata gatttgataa
   105481 acaactgaat ggtgccacca tttttgttcc atgattttcc tcctataaat ggatgtttct
   105541 attttttgct taatcattta ttttagtctc tctcactaaa aattgtaacg ctttcaaata
   105601 gctattgcac cttgttatcg gtaacagaat aaaacaaaaa aacagctgag gaaattccct
   105661 caactgtttt ttgaaaggat tgatttatgc agggaagaat gctgatttca caacttcttt
   105721 ataaacggtg ttcactcagc tgtcccttgg tattagctca aaaaacggca aaatataaga
   105781 aactattttt tcgttttttt ctctaatact caggccagaa cgctgatttc acaacctctt
   105841 tatttcacat tcaatatcca catgaatccc acaaatacaa agaacagacc atacatgatt
   105901 ggagatactt cttttcctct tttagctgct aacatggtaa ttggatagaa gataaaacct
   105961 aatgcaatcc catctgaaat actataagtc aaaggcatgc cgatcaagat caagaatgaa
   106021 gggatcgcaa tctctaagtt tccccattcg atttgtcgca aagattgtgc catcaatact
   106081 cctaccataa tcaatgctgg tgcagttact tgtgaagtaa caactgatag taatggagaa
   106141 aagaacaagc ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc tgaacgtcct
   106201 ccaacggcga taccagctga tgattctaca aatgcaccaa caggagaagt accaaataag
   106261 gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt tccctacacg aggcatcttg
   106321 ttgtctttca tgaaacctgc ttggtttgct aatccaacta aagttcctgc tgtatcaaag
   106381 aaagtcacaa gcaagaatgt cagcactacg acccacattt gaagagaatt gatgtcccct
   106441 acatgtttta atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc aacaatttca
   106501 gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc catcccgatg
   106561 aagatgccac ctggtacacg ccgaacaagc aagatagcag ttacgactaa tccaaaaatc
   106621 gtcaaccaag tagaaccaac gttgagtgat cctaatccta ctaatgtaga ttcattcgaa
   106681 acgatgatcc caccttcgct cagaccaagg aaagcgataa atagaccgat accgccagaa
   106741 atcgcatatt tcaaatctgc tgggatcgca tcaatgatca tttcccgcag cttgaagatt
   106801 gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt ttcccatgga
   106861 atccccattc ctaaacaaac agaataagca aaaaaagcat tgatcccaag agcaggtgcc
   106921 gttccaattg gataacgggc taataccccc attaaaacac agccgatcgc actggctaat
   106981 gcagtcgcag taaacactgc tccttcatcc attccagaag cacctaatac agttggattt
   107041 acaaataaga tataagccat tgagataaat gttgtaaatc ctgccagtac ctcacgtttt
   107101 accgtggtgt tcaatttttc tatttcgaag taggaaagta ttttttcctt catcttccat
   107161 ccccctaaat ccgaacatta taaataaagt ccgttaaaat ttcgatttat taataaaaca
   107221 acgtgtttta tcatatacta aaacaaaaca taagtaaaca aaaaattcgg attttaagca
   107281 aaatccgaac gttccatctg attaaatccc tttatctcaa agttcatgaa cattcacttt
   107341 ggattacaag aatttcttta ttgtgttaat aaagtttctt cattctagtc tcaaaaatca
   107401 atagtgtgat ttgattttac catttcgttt ctgttcttct ttgatgaatt cttttgtatc
   107461 gacaggattc agattattta gtccgccaaa acctgtatcg accatttttc cccagccttt
   107521 tgtcttcttt tttcctgatt tgagattctt tgctcgaagc ttctcactat gttttgctcg
   107581 ccgatacttc aaataacctt ctaatcttcc cattagatcc actgtttttc ctccttttta
   107641 ttcaatcaga cgactcagct tttcctataa caaaataatt gccttcaggg tcagaaaaat
   107701 tgaaagtcaa gatgccattg ttatctgtga tttctcctgg ttcttccatg ttctcgtaga
   107761 tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg tggtcctaat acctctggtg
   107821 aaaacttctg cacaaatact tttgggaata cgcctaattc taattctttt gagatgctga
   107881 gtacaatact cttaaattct tctggcaatt cgattgtttc agaaatttct gcaccaattt
   107941 tttctacaaa gaatcggcaa atcatctcta cgtcattgac ataaagcatc acacgtgttt
   108001 tttgtatcat catttgaccc ttctttctat ttgattttct ttcctgtcta taaactgatt
   108061 ccattatatc acgaaagcgc aaacaccttg tgtttgatac tattcaagaa aaataaagca
   108121 atagtttcta ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa cggcaatgaa
   108181 tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct ttggttagtg
   108241 tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac gcttcgattg
   108301 cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc accgctgctt
   108361 gtgtttcttc cgtcagcatc gttctttggg cttcacgcca gttgagacag cgacagatcg
   108421 ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg gaatctgcac
   108481 ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt cctgcgatct
   108541 tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta ttatagatat
   108601 ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc agtaaagctt
   108661 caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga cgccactgag
   108721 caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct tcttttgctg
   108781 cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg ttatcgatac
   108841 catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa ttggtatcca
   108901 ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga tgaatagata
   108961 gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga taaatccttt
   109021 tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc aaatggctgt
   109081 tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac cgataccttg
   109141 atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat cagccatttc
   109201 ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt gatcgatact
   109261 cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt ctagtgcata
   109321 agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa atgtatctgt
   109381 aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca tttttcgaat
   109441 ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt ttttgacaaa
   109501 attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca attgttttgc
   109561 ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt ctcgaatgat
   109621 gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt ttttattttc
   109681 tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg ctcgggcagt
   109741 cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc caggattttc
   109801 gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt tgaattcgat
   109861 attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga tttcagccat
   109921 ttcatcccac ctttattttg tttgtaagac tagttcacct ctttttgttg caaatgcaac
   109981 tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat tcttttcttc
   110041 ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat aagtaactgt
   110101 ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa aaatcccatg
   110161 ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc acaagcaaaa
   110221 acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt atcaaaaact
   110281 gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc tttacgcaca
   110341 ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca ttctttcgtc
   110401 cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg aacatctact
   110461 tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc gaaagtttct
   110521 tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa acgctcttta
   110581 tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag tcttttgtat
   110641 tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg actatgtaat
   110701 caagtataga agactggttt aattacggca aataaatata atgaaatctg caataaataa
   110761 aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct gtgaataaat
   110821 ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat atgacgagct
   110881 attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa cctccccact
   110941 aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca aacatgagaa
   111001 gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc acgacctctc
   111061 ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt tacttattgt
   111121 cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga gtatagtctg
   111181 ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg tcggcgatga
   111241 tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct tccaatacgt
   111301 caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca cgatcacgtt
   111361 tgtaccgacg ttgccataac acttcaaagt ctgctgttag gcgaatcgtg atcacctgat
   111421 atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta aaaggatact
   111481 cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg taatagaaag
   111541 gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac ccgatggatt
   111601 ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga aatcgttccc
   111661 gtagtttatt cataagatat gtttttcctg taccaggact accagccaga agaatgaaat
   111721 actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat aaacagtcca
   111781 atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt atggcgaaga
   111841 gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca gttgactaaa
   111901 agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg ataatcttct
   111961 tctttcacac ctgttcctcc atggataact agggggacat caactactgc atcgatttct
   112021 ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt cgtaccgata
   112081 gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc ttccggtctt
   112141 gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc gattgttcca
   112201 agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc ttttgtcttc
   112261 aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt atatccgctg
   112321 tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa gactgcatca
   112381 accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat gccgatatat
   112441 ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt tgctgcgcct
   112501 agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt aaaattcaac
   112561 tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg ccaccttatc
   112621 tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct catccatttt
   112681 gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg cacgcttctg
   112741 gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat tttccaaact
   112801 cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt taagtccaac
   112861 ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct cttgtttcaa
   112921 ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt tttcttcttt
   112981 tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca tcattttccc
   113041 tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata agagtgctgc
   113101 ggctactgta tctacatcgg ttgctgtagc attgttgaat cccatcgaat taaagatcgt
   113161 tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc caatgatcgc
   113221 tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg caaagaaatt
   113281 ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa acatggcaat
   113341 aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc tataaggaaa
   113401 gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag ctatccctcg
   113461 aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata catacacacc
   113521 cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat ctcctgataa
   113581 ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg ggaccatcac
   113641 gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca acttgatatc
   113701 ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac cggcttcaaa
   113761 taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag tgattttacg
   113821 aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga taaaaccacc
   113881 gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg tcgtcatcgt
   113941 tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg tgaaacgagc
   114001 caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc tacctaaacc
   114061 caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc cttgcatatg
   114121 aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga tgctcgaacc
   114181 ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga cttgtcctgc
   114241 tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga tcgatgcctg
   114301 agtcaaaata ttttcgatga taaaattgat aacgcccatg ttttgatgtg caccccaaaa
   114361 gttagactag aaatctaatt aaaaggggtg ctttttttgc ggaaatatac atttgttttt
   114421 aagaaaaaag tagtttcaga ctatttaaat aacgaaggcg gctacaaata tcttgcacat
   114481 aaatatcaaa taaatcgtac actggttaga cattgggtaa ggatttataa ctatcatggt
   114541 tgggaaggct tggttggagg tggcaaaagc tacactacaa aatttaaact tgatgttata
   114601 gaatatatgg aaacaaatgg tctttctatc caagaaactg ctaaaaaatt taatatcggt
   114661 tcaaatagaa ctctaagtaa atggatagag caatatgaag aaggcggtgc ttcttcactt
   114721 gagagccaaa aaaggggcag aaaaattagt atgaattcca agctaaacat tcctaaaaaa
   114781 cttaaagatg agtctcttga agaagaagtt attcgtttaa gagcagagaa cgcatattta
   114841 aaaaagttag aaaccttgat tcaagaacaa gatttgagca agaagaaatc aaggttaaaa
   114901 caatccttca gctcaaaaaa gaatttaaat tgaatctatt actttcaatt gcacaattag
   114961 cgaagtcaac ttattattac tgggtaaaaa aattagataa gccagataaa tatagcaaga
   115021 taaagcaaga aattacagcg attgtaaaag aatcaagaaa ctcttatggt tatcgtagag
   115081 tcactttagc gttaaagatg aaaggatata caatcaatca taaaacagtt agaaaattaa
   115141 tgtcccaaat gggacttacc tgtcaaatcc gaataaagcg atataaatct tataaaggaa
   115201 cagtaggaaa aattgccaag aatgtgttaa aacgaaattt ttcagtagat acacccaata
   115261 aaaaatgggt gacagacgtc actgaattca agataaaagg aagaaaaatc tatctatctc
   115321 ctatacttga tttatttaac ggagaaataa ttagttatag tatatcaact agtccaacat
   115381 ataaactgat tgaagaaatg cttcaacaag caattaaaaa aaagggaact gagggttcac
   115441 tgattctaca ttcagatcaa ggatggcagt atcagatgcc acaatatcaa aaaaaattaa
   115501 aagagaataa cattattcaa agcatgtcta gaaaaggaaa ttgcttagat aattctgtaa
   115561 tagaaaattt ttttggtgtg cttaaatcag agttttttta tcgagaaaaa ttccgatcaa
   115621 ttgagatatt tcaaagtaaa ttaaatgagt atattaggtg gtataataac aaaaggataa
   115681 aactaaagtt aaatggacta tctccagtag aatatcggaa acagtccatt aaatagtt
//
LOCUS       DAYYBG010000002        80097 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000002 DAYYBG010000000
VERSION     DAYYBG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.002"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(172..912)
                     /locus_tag="ACHAUN_000101"
     CDS             complement(172..912)
                     /locus_tag="ACHAUN_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458841.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1042..1989)
                     /locus_tag="ACHAUN_000102"
     CDS             complement(1042..1989)
                     /locus_tag="ACHAUN_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8458842.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2118..2882)
                     /locus_tag="ACHAUN_000103"
     CDS             complement(2118..2882)
                     /locus_tag="ACHAUN_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8458843.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2886..3275)
                     /locus_tag="ACHAUN_000104"
     CDS             complement(2886..3275)
                     /locus_tag="ACHAUN_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458844.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3410..3952
                     /locus_tag="ACHAUN_000105"
     CDS             3410..3952
                     /locus_tag="ACHAUN_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8458845.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4100..4732
                     /locus_tag="ACHAUN_000106"
     CDS             4100..4732
                     /locus_tag="ACHAUN_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458846.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4866..4991)
                     /locus_tag="ACHAUN_000107"
     CDS             complement(4866..4991)
                     /locus_tag="ACHAUN_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8458847.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5125..6018)
                     /locus_tag="ACHAUN_000108"
     CDS             complement(5125..6018)
                     /locus_tag="ACHAUN_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8458848.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6200..7126)
                     /locus_tag="ACHAUN_000109"
     CDS             complement(6200..7126)
                     /locus_tag="ACHAUN_000109"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8458849.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7211..7972)
                     /gene="pflA"
                     /locus_tag="ACHAUN_000110"
     CDS             complement(7211..7972)
                     /gene="pflA"
                     /locus_tag="ACHAUN_000110"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8458850.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8216..10447)
                     /gene="pflB"
                     /locus_tag="ACHAUN_000111"
     CDS             complement(8216..10447)
                     /gene="pflB"
                     /locus_tag="ACHAUN_000111"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8458851.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10724..13174)
                     /gene="parC"
                     /locus_tag="ACHAUN_000112"
     CDS             complement(10724..13174)
                     /gene="parC"
                     /locus_tag="ACHAUN_000112"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8458852.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13186..15231)
                     /gene="parE"
                     /locus_tag="ACHAUN_000113"
     CDS             complement(13186..15231)
                     /gene="parE"
                     /locus_tag="ACHAUN_000113"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8458853.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15397..15831)
                     /locus_tag="ACHAUN_000114"
     CDS             complement(15397..15831)
                     /locus_tag="ACHAUN_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8458854.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15958..16596
                     /gene="plsY"
                     /locus_tag="ACHAUN_000115"
     CDS             15958..16596
                     /gene="plsY"
                     /locus_tag="ACHAUN_000115"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8458855.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16728..17603)
                     /locus_tag="ACHAUN_000116"
     CDS             complement(16728..17603)
                     /locus_tag="ACHAUN_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8458856.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17685..18476)
                     /gene="codY"
                     /locus_tag="ACHAUN_000117"
     CDS             complement(17685..18476)
                     /gene="codY"
                     /locus_tag="ACHAUN_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8458857.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18494..19894)
                     /gene="hslU"
                     /locus_tag="ACHAUN_000118"
     CDS             complement(18494..19894)
                     /gene="hslU"
                     /locus_tag="ACHAUN_000118"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8458858.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19908..20456)
                     /gene="hslV"
                     /locus_tag="ACHAUN_000119"
     CDS             complement(19908..20456)
                     /gene="hslV"
                     /locus_tag="ACHAUN_000119"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8458859.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20493..21398)
                     /gene="xerC"
                     /locus_tag="ACHAUN_000120"
     CDS             complement(20493..21398)
                     /gene="xerC"
                     /locus_tag="ACHAUN_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8458860.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21596..22972)
                     /gene="trmFO"
                     /locus_tag="ACHAUN_000121"
     CDS             complement(21596..22972)
                     /gene="trmFO"
                     /locus_tag="ACHAUN_000121"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8458861.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22941..25019)
                     /gene="topA"
                     /locus_tag="ACHAUN_000122"
     CDS             complement(22941..25019)
                     /gene="topA"
                     /locus_tag="ACHAUN_000122"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8458862.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25141..25998)
                     /gene="dprA"
                     /locus_tag="ACHAUN_000123"
     CDS             complement(25141..25998)
                     /gene="dprA"
                     /locus_tag="ACHAUN_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8458863.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26054..26821)
                     /locus_tag="ACHAUN_000124"
     CDS             complement(26054..26821)
                     /locus_tag="ACHAUN_000124"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8458864.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26814..27674)
                     /gene="ylqF"
                     /locus_tag="ACHAUN_000125"
     CDS             complement(26814..27674)
                     /gene="ylqF"
                     /locus_tag="ACHAUN_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8458865.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27934..28332
                     /locus_tag="ACHAUN_000126"
     CDS             27934..28332
                     /locus_tag="ACHAUN_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8458866.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28569..29111
                     /locus_tag="ACHAUN_000127"
     CDS             28569..29111
                     /locus_tag="ACHAUN_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8458867.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29194..29745)
                     /gene="lepB"
                     /locus_tag="ACHAUN_000128"
     CDS             complement(29194..29745)
                     /gene="lepB"
                     /locus_tag="ACHAUN_000128"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8458868.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29897..30124)
                     /locus_tag="ACHAUN_000129"
     CDS             complement(29897..30124)
                     /locus_tag="ACHAUN_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8458869.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30121..30639)
                     /gene="msrA"
                     /locus_tag="ACHAUN_000130"
     CDS             complement(30121..30639)
                     /gene="msrA"
                     /locus_tag="ACHAUN_000130"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8458870.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30659..31372)
                     /locus_tag="ACHAUN_000131"
     CDS             complement(30659..31372)
                     /locus_tag="ACHAUN_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8458871.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31375..32271)
                     /locus_tag="ACHAUN_000132"
     CDS             complement(31375..32271)
                     /locus_tag="ACHAUN_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8458872.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32429..33271)
                     /locus_tag="ACHAUN_000133"
     CDS             complement(32429..33271)
                     /locus_tag="ACHAUN_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8458873.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33403..34056
                     /locus_tag="ACHAUN_000134"
     CDS             33403..34056
                     /locus_tag="ACHAUN_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8458874.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34119..34646)
                     /locus_tag="ACHAUN_000135"
     CDS             complement(34119..34646)
                     /locus_tag="ACHAUN_000135"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8458875.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34666..35613)
                     /locus_tag="ACHAUN_000136"
     CDS             complement(34666..35613)
                     /locus_tag="ACHAUN_000136"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8458876.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35625..37508)
                     /locus_tag="ACHAUN_000137"
     CDS             complement(35625..37508)
                     /locus_tag="ACHAUN_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8458877.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(37671..39749)
                     /locus_tag="ACHAUN_000138"
     CDS             complement(37671..39749)
                     /locus_tag="ACHAUN_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8458878.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39762..40037)
                     /locus_tag="ACHAUN_000139"
     CDS             complement(39762..40037)
                     /locus_tag="ACHAUN_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458879.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40399..40854)
                     /locus_tag="ACHAUN_000140"
     CDS             complement(40399..40854)
                     /locus_tag="ACHAUN_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458880.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(41008..42216)
                     /locus_tag="ACHAUN_000141"
     CDS             complement(41008..42216)
                     /locus_tag="ACHAUN_000141"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8458881.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42328..42660)
                     /locus_tag="ACHAUN_000142"
     CDS             complement(42328..42660)
                     /locus_tag="ACHAUN_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8458882.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42808..43683
                     /locus_tag="ACHAUN_000143"
     CDS             42808..43683
                     /locus_tag="ACHAUN_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8458883.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43765..44346)
                     /locus_tag="ACHAUN_000144"
     CDS             complement(43765..44346)
                     /locus_tag="ACHAUN_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8458884.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44352..45611)
                     /locus_tag="ACHAUN_000145"
     CDS             complement(44352..45611)
                     /locus_tag="ACHAUN_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8458885.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45613..46659)
                     /locus_tag="ACHAUN_000146"
     CDS             complement(45613..46659)
                     /locus_tag="ACHAUN_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458886.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46894..47169)
                     /locus_tag="ACHAUN_000147"
     CDS             complement(46894..47169)
                     /locus_tag="ACHAUN_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8458887.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47412..48722)
                     /gene="der"
                     /locus_tag="ACHAUN_000148"
     CDS             complement(47412..48722)
                     /gene="der"
                     /locus_tag="ACHAUN_000148"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8458888.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48904..50133)
                     /gene="rpsA"
                     /locus_tag="ACHAUN_000149"
     CDS             complement(48904..50133)
                     /gene="rpsA"
                     /locus_tag="ACHAUN_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8458889.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50268..50948)
                     /gene="cmk"
                     /locus_tag="ACHAUN_000150"
     CDS             complement(50268..50948)
                     /gene="cmk"
                     /locus_tag="ACHAUN_000150"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8458890.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(50997..51632)
                     /locus_tag="ACHAUN_000151"
     CDS             complement(50997..51632)
                     /locus_tag="ACHAUN_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8458891.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51698..53101)
                     /locus_tag="ACHAUN_000152"
     CDS             complement(51698..53101)
                     /locus_tag="ACHAUN_000152"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8458892.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(53088..54119)
                     /locus_tag="ACHAUN_000153"
     CDS             complement(53088..54119)
                     /locus_tag="ACHAUN_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8458893.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54150..54371
                     /locus_tag="ACHAUN_000154"
     CDS             54150..54371
                     /locus_tag="ACHAUN_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8458894.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54536..55273)
                     /locus_tag="ACHAUN_000155"
     CDS             complement(54536..55273)
                     /locus_tag="ACHAUN_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8458895.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55426..56766)
                     /locus_tag="ACHAUN_000156"
     CDS             complement(55426..56766)
                     /locus_tag="ACHAUN_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8458896.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(57003..58526)
                     /locus_tag="ACHAUN_000157"
     CDS             complement(57003..58526)
                     /locus_tag="ACHAUN_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8458897.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(58904..59515)
                     /locus_tag="ACHAUN_000158"
     CDS             complement(58904..59515)
                     /locus_tag="ACHAUN_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8458898.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(59638..59763)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(59845..60561)
                     /locus_tag="ACHAUN_000159"
     CDS             complement(59845..60561)
                     /locus_tag="ACHAUN_000159"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8458899.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(60567..61136)
                     /gene="scpB"
                     /locus_tag="ACHAUN_000160"
     CDS             complement(60567..61136)
                     /gene="scpB"
                     /locus_tag="ACHAUN_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8458900.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(61120..61890)
                     /locus_tag="ACHAUN_000161"
     CDS             complement(61120..61890)
                     /locus_tag="ACHAUN_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8458901.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(61887..62258)
                     /locus_tag="ACHAUN_000162"
     CDS             complement(61887..62258)
                     /locus_tag="ACHAUN_000162"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8458902.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(62277..63164)
                     /gene="xerD"
                     /locus_tag="ACHAUN_000163"
     CDS             complement(62277..63164)
                     /gene="xerD"
                     /locus_tag="ACHAUN_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8458903.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(63260..63733)
                     /locus_tag="ACHAUN_000164"
     CDS             complement(63260..63733)
                     /locus_tag="ACHAUN_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8458904.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(63838..64701)
                     /locus_tag="ACHAUN_000165"
     CDS             complement(63838..64701)
                     /locus_tag="ACHAUN_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8458905.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(64897..66996)
                     /locus_tag="ACHAUN_000166"
     CDS             complement(64897..66996)
                     /locus_tag="ACHAUN_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8458906.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(67216..68325)
                     /gene="rpoD"
                     /locus_tag="ACHAUN_000167"
     CDS             complement(67216..68325)
                     /gene="rpoD"
                     /locus_tag="ACHAUN_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8458907.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(68349..70190)
                     /gene="dnaG"
                     /locus_tag="ACHAUN_000168"
     CDS             complement(68349..70190)
                     /gene="dnaG"
                     /locus_tag="ACHAUN_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8458908.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(70372..72687)
                     /locus_tag="ACHAUN_000169"
     CDS             complement(70372..72687)
                     /locus_tag="ACHAUN_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8458909.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(72872..73732)
                     /locus_tag="ACHAUN_000170"
     CDS             complement(72872..73732)
                     /locus_tag="ACHAUN_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8458910.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(73791..74423)
                     /locus_tag="ACHAUN_000171"
                     /pseudo
     CDS             complement(73791..74423)
                     /locus_tag="ACHAUN_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            74590..75351
                     /locus_tag="ACHAUN_000172"
     CDS             74590..75351
                     /locus_tag="ACHAUN_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8458911.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            75377..77968
                     /gene="mprF"
                     /locus_tag="ACHAUN_000173"
     CDS             75377..77968
                     /gene="mprF"
                     /locus_tag="ACHAUN_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8458912.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(78156..79343)
                     /gene="tuf"
                     /locus_tag="ACHAUN_000174"
     CDS             complement(78156..79343)
                     /gene="tuf"
                     /locus_tag="ACHAUN_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8458913.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            79711..80067
                     /locus_tag="ACHAUN_000175"
     CDS             79711..80067
                     /locus_tag="ACHAUN_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8458914.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        21960 a        16839 c        13737 g        27561 t
ORIGIN      
        1 aatagtttgt ttttttattt tgccattatt ttcttactta ttgggagaaa aaattcgctt
       61 aaactcaaaa aaatttgagg atacgatata aactttcgga tagagtataa aataaggaaa
      121 cgccgcagcg tttcctttcc aactgtagcc gaatataaat ttgtatatca cttatttaaa
      181 atagctattt tctcccaaag agtttccttg atcatcgatc aatccttgta atgagcgttc
      241 taagatacca tggctttttt tcgatttttt ttcttctgac tgctttgttt cctctaacgt
      301 ttttgttcga ttgaatgttc gtacagacgg ctcaccattt cttttttctt gatacggcgt
      361 cggttctgta tcgaacaaca gataagagtc atccgttttt ttcatcgatt cgattaaatg
      421 atcctcagaa attttgcttt ctctagggtc tgggataatc gatgcgggta catatttagg
      481 aacaaaataa gaacgctccc gcaccttcgt gtcaacagat gcgtttcgtg tagtatgctt
      541 ggactctgtc cttgtttcac tttgtctgac agaacgagag gaatcaccaa aaagtttttt
      601 tcgtcctgtc gatgtgattt ctgattggta atggccggta taatccggta attttccttt
      661 atgcttttcc aaattttcct tacgcggagc tgacttcaat ccatttgttg cacgacgctt
      721 gcgacggatt ttctcggtgt tcgtgttcgt gaaatgaaat tctttcatct gttcaaacgg
      781 ggactgattc tgttcagtca aaagatcttc ttgtccgtca aataatactc gcttcttgtc
      841 gttggttaat tttacgccta actcatcatc gtacgcagga aaacggaaat gttttcgtct
      901 gatttctttc attcaaacca tccttctcaa aaatggaact tttctcaata atagcacaga
      961 ttcattgttt tcagtagcaa tgcgctgatt attttaagaa aatagcgatg aatttcataa
     1021 atatatatat agtaataaga cttaattcgt tgtctctctg ataactagtt caggaaaaaa
     1081 acgacgataa ccttgactcg cccctttttc ttctattttt tttaacaaca tttctccaca
     1141 tacttcaccc atacgtataa tatcttgctt gatcgttgtc aatttaggtg aactgatttg
     1201 gtctaaaaat accccatcaa atccaatcac accaaattct tcgggcaagt gtcctccagc
     1261 atttataatc cctcttactg ctcctagtgc tagccgatct gaactacata caaaacaagt
     1321 tttatctgta aaatctgccc agttttctgt catatatctt tctacttctg tcgaatgatt
     1381 tggaaaccgc aagattttac taggaagatc aacttccttc atcacagata aataaccagt
     1441 ttcccttgaa atttcaaaag gctcgtccac ttcgataccg atataaataa tcgtttgata
     1501 atctctttct aaggcatatt gcgttgcttt tgctgtacca tattcattat cgctgtctac
     1561 ataatcatat ccttcagaat tttccccaaa caagacaact ggtttttcca aacggtcgat
     1621 ccaatcgaag tctttttttc tgacacctgt gatgatgtat ccatcacaag aaccgacgtc
     1681 gaaggcatca tttgtaacta attggagaga ataatgcgct ttaccgatcc gtttagcgat
     1741 tcccatcagc aaattcatgt aatagggctc agttgtgtcg atttcttcca gtataaataa
     1801 tttgatgatc tgcgaccgat tcgtgacaag tgcttttgca gcaatatttg gacggtaatc
     1861 cagttcagcc attgctgaga aaactaactc tttcaattcg tcagtcacct gttccggatg
     1921 attgatgact cgagagaccg tcatttttga aacattggca cgttttgcaa catctgctaa
     1981 agtgggcatg ctctcttcct ttctaacatg ttgtcattct tactatcaat tataccaaat
     2041 tttcctaaca gagtttatta tagcccaaaa taaaaaacgg aaaatcctct atcgtcatga
     2101 gtgattttcc gtttcaatta tttatacttt tttatcaaat tttttgctaa aaaagtggaa
     2161 gcttcttcca ttagtggtat ttgtaccata tgtcgctgtg tttttgagga acgaaaaaca
     2221 atttcttttc cgtaagactt atgccgattt ttttctacaa atcgttccgt ttgtatataa
     2281 gggatttttt cgtcagcttg tccatgccaa aagaacagag gacgtccagc taacttttcc
     2341 ggctgcaatg acaaatcata gctcttaacc catgaaatca aatcaaaata gtcttttggt
     2401 acgacataat tatattccga tgcgcgcctt acgatttctt ttccatattc caaaggtgca
     2461 ggggaaccca tcaaacacac gcccgctact atatgaggat gtttagccaa caaggcgcat
     2521 gttgtcattc cccccatcga aaccccgcca actgcaagct gatcatgtag aagacctctt
     2581 ttttctaaat gcttgataag tgtgtcaaat tcaaacagat ttccgtagat actatcccaa
     2641 aatgtatagg atggaataga agaaaccggc tgtttgcgct ccccatgatt catcgcatct
     2701 ggcaggatca cacgaatgcc ctgctgcgca atttttcttc cttgcgtcaa aactaactcc
     2761 ttacttgtct gccatccgtg ataatagatg acaacgggaa tacgttcctc tcttaattct
     2821 ttcagaacaa cttctaacac tggaatatga tggacagaac gtttcaatat aattggattc
     2881 aattgttact cacctatttt cattaaattt cctgatcgcc atagcaacca tgctccaaca
     2941 gttgtctgga acaggttcag tgtaccaaat aatataaatg caggactgaa tggctcggga
     3001 aataagcaca gagaacaaag tcccaccact cctaatgcta gcgaaaggag tggataaata
     3061 gaaaaattca aaaaactggc aacgctaccg aaaatattac caataaacag tatcaaaaag
     3121 aaaaaaacgc ttaaaattaa accatctgtc agcgacatct gaaaaacaat gttctctacc
     3181 tcaattggct gttgaatcga atagacacct aagtatatac tgagaaggag acttagcacg
     3241 tggctggcac aaaacacacg attgaccgtt ttcattttaa tcacctaacc tatctttata
     3301 attctattat aatcatttta tcattttttt gtatgtataa agtttcaatt tttcttcact
     3361 tacgattttt tttgatattg ttgttatata aatgaaagga gttttacaaa tgattgatcc
     3421 aaaaatgttg gggatgatct tcctcattaa ttttgcttat atcactctga acacgttacg
     3481 tttcatgttg acaatgaaag gctatcgact gattgctcca cttgtcagta tggtcgagat
     3541 cacgatttat atcgtcggct tatcccttgt tttagatcgt ttagataatc cactgaattt
     3601 atttgtctat gcgttaggtt acgcagtagg tatcagtgtc ggaattttga ttgaagacaa
     3661 attggcatta ggatacatca tggtcacaac gattttgcca gcaaatagcg cagaagataa
     3721 aagtttgccg ggtatactga gagaacacgg ttatggtgtg acacagactt ctgcaatggg
     3781 gctggaaggt gagcggctag ttctcgaaat actttctcct agaaaaagcg aacgtgaact
     3841 gtatcgcctg atcaaagaaa tcgaagagcg tgcttttatc atttcttatg agccaaaata
     3901 tatttcaggt ggtttctgga ctaaaaaagt gagaaaacga caaggaaaat aactcacaaa
     3961 cttaaaaact gtctaatatc taaattaaat tttaaatgtt agacagtttt tatttattat
     4021 tttgattaaa aaacatcgac atatactcat caatttgtta ttatacttta tatacttatc
     4081 aatttaattt ggagatatta tggaaattca acgtagaacc actaattttt tacatttagg
     4141 ctcttttttt gcaactttga cgatcttatt ttttctatgt tattcatacc ttaagacctc
     4201 acatgcgcct tctagtgcat taggctggtt gcttttggat cattctgata tcacagaagc
     4261 cgaagaaaat ccgttttttt tgattttgtc ttctctttgc ggcttactgt tttctgttta
     4321 tttcggtgcc tatttttttc atcgacatcc tggtacgtta aacgaagctt caagcgtatt
     4381 gattgcattt aaaacgtttt cttttttagc aattcttttt tattcgttta atcacaatac
     4441 gcttttcttc tttatcatga atggtttagt cgtgatttta tccattttta cttattttat
     4501 tttttcactc atttttagcc aattgaaatg tccgctcttc tttcgattag ttcctgtcac
     4561 ctgttttatt tacagcttgt tttccttttt tgtccttgct ttaatgccac tgaccaaccc
     4621 ttcgacgatc caagtcgcag gaaatctgtt ggatatgctc ttcatcgtca gtctaggtgt
     4681 atttatgtgg caaagaaaaa acaaacaaaa taaaaagcat tttgaagcct gactattacg
     4741 acttcataaa tgctttttta gttttaaaca cttcaatcta aaagaataac ccaagattgg
     4801 ataaattatt aatgactcgc cattcgtttc ttagtgattc tggattatca aatggacttc
     4861 ctaatctaat cagctagttc ttcccataaa atgccatact tgactgaatc ataatattga
     4921 ttatttaaaa atctaacttt tcttatcact acttcctttg tcattccaca tttctcacca
     4981 tttttttgca ttcctttgtt tccagaccat gtagtgaatc taataggtgg tagataggca
     5041 aaatactcaa ataattcttt taaccataaa ggaagagaaa ggctcctcct gcaagagtca
     5101 gtataatttt tgcttactgc tctcttaggt tttactcact aaaactgttc catattcttt
     5161 gatataagaa aatgcatgcg gtataaattt cttgctcagc tcagaaaaga tttgtcgttt
     5221 aagaaaggct atttcctcca ttgtcaatga aggatccaat gttggtaatt caatctcaaa
     5281 attgtttttt tcgacaatag gtaattctaa tgtgaatgat tcttcttcct ttatagtatt
     5341 cgtcacaagt agagactgat ggaaaatact aggccggcga tttttatatt tattcttacg
     5401 tatcctgtca acgataactt ctatttgatc aaaaaagtac gattcattta cgtctccaat
     5461 aagttcggct aattctttga atgccttcgt tacaggaagc tgattcttct gtacaaaaaa
     5521 gtaattagcc aaagtaagcg ggaagtgttc tatagaggaa agtgtgataa aacggtactt
     5581 ctcatttcct gcagttgtcc tcagcaattc tgtctctcgg ctgtttctta ctccataaaa
     5641 atacgcttct ttccgttcat cttcgaaaca atacgcccaa ataacttctc ccatcgctaa
     5701 tttttgttca tcaacagaga gtctcttcaa ttgttcagct atggtttcag cagccgaagt
     5761 ggcttgttgc aagtaatctt taggatcaaa tattggaaag ttcaaatgat atcttcgttc
     5821 ttccctcttg attaatcctg catcgatcaa taaatcaaga aaatgggaaa atttattttg
     5881 cgaaaattct tttttcaatt ctcttagaat cgtttcttgt tctcgatgtt catatagata
     5941 atcggtcaat gaagaaaaaa gcggatcatc ggtcagtttt tgataaaagt tttccttatt
     6001 ttttgaataa tagttcatat atgttctctt ttctatccgt tttattttac ttaaaaggct
     6061 tttacgtaaa aaggaggaag aacaagagtc ataattgact gttattctcc tccatttctt
     6121 cggcctttag tatgttctat gtttcttttt atatcaatcg cttatccatt cattttgaat
     6181 gcttcatact accatttgat tagctaaata cagccgtcaa ttgaggtacg acttgttttt
     6241 tacgtgaaac gacaccttta agcaaagcac ggttgttggt caaagttgtt tggaaagctt
     6301 cttctacttt ttcttttcct ttaccagcaa caagtaattc tgaatcactg tctaagatgt
     6361 ttgtgacaac taatacgaac agatcgtagc catttgcttt catttcatct tccattgctg
     6421 cttctagctc tgcttgtcga tccatcactt cattaaggtc cactgtattg acttgagcga
     6481 tacgaacgtt ggaactgccc attgggaaac tttttgcatc caaatcaaga agaaccgcag
     6541 ctgatttatc acttagattt gttcctgctt tcaacatttc aagtccataa gtgtttagat
     6601 cgacttcagc gattgctgca agttcttttg cagctgcttc atcttcaggc gtacatgttg
     6661 gtgatttgaa caacaatgta tctgaaatga tagctgagag catgattcct gcgatttctt
     6721 taggaattgt tacttggttt tctttatata gttttaaaat aatcgtactt gtacatccaa
     6781 ctggttctgc acgatagtac aatggattag ctgtttcaaa gtttgcaata cgatggtggt
     6841 ctactacagc taaaatattt acttcgctga tatctgaaat actttgttgg aattcattat
     6901 gatccacaag catgacatct gcattttctg ctttagctga ctctactact cgtggtgcag
     6961 atagctgaaa gtggtctaat gcgtattgtg tttcttcatt aggtgtacct agagcaactg
     7021 cttctgtttc tttacctaat tcattttgca agtgtgaaaa cgcaatggct gccccgattg
     7081 catctgtatc tggattttgg tgaccaaaaa ctaaaatttt tgacatgtca ttcgctcctt
     7141 tgtgatttct cctatttaat aatagcaaaa aaacggaaag aagcaatact tatcttcttt
     7201 ccgatgcgac ctatcgcgtt aaatattttg tatactcttc tgtatgaagc aatcgtttag
     7261 cattttcgac acgttctttt gtaggtggtt cgatcccttc taatggatat ttcaaaccaa
     7321 gtgtttccca cttgtatttt cccatcgtat gataaggaag aatctctact ctgtctacat
     7381 ttgacaaggt ttgaataaag ttgtctagtc ggatcaaata ttcatcataa tcacttcgct
     7441 caggaactag tacatggcga atccaaaccg gtttgttgat ttttgacaaa tattgagcca
     7501 tttctaaaat attttcattg tcccaatgcg ttagtttttt atgctcttca ttatcgatgt
     7561 gtttgatgtc aaataacagt aaatctgtat acttcatcaa ttcttgaaaa cgtgaaaaga
     7621 aaggttcttc atacgtaaaa ggttttccgc atgtatccaa tgtagtatgc atcttccgct
     7681 cttttgcttt tttaaatagt tcgatcaaaa aatcgatctg caatagaggt tctccaccac
     7741 taacagtaat accaccttta tctccccagt aaggtctgaa gcgttctgct ttatctaaaa
     7801 gttcatctgc tgtgtattct ttccctccgc caatattcca agtatctgga ttgtgacaaa
     7861 attggcaacg catgcggcat ccttgcataa acacgacaaa gcgcagtcca ggtccgtcta
     7921 ctgaaccgaa agtttcgata gaatgaacat agccgattgt tttttcttcc atttaagttc
     7981 acatcttttc tttgatttca ttttaagtac aagttagctg tcgaagcgaa gctttctgaa
     8041 caaaattttt cgcaattgcc gcttcgttat gtgtaaaatt tacagggacg gaacatacaa
     8101 gtcctgcccc tgtaaatcgt ttctttcaaa taagcatact gattattcga ttttgatgcg
     8161 atggctattc aacgtccttt cgacgctaag tcgaaaaact tttttcacga actttttaca
     8221 ttctgtcgtg tgaagttctc atcaagacat ctaattgttg ttctcttgtc aagtcacgga
     8281 atttaactgc ataaccagat acccggatcg tcaagttcgg atatttcatt ggattttcca
     8341 ttgcatcacg caataaatca ttactgaata cattgacatt caaatgataa gctccttttg
     8401 agaagtatcc atctaagaca ttacgcaagt tttcgattct tgtatcttgt tcttttccaa
     8461 gacctgaagg attgattgtt tgggtatttg aaattccatc caatgcacag ccgtaattca
     8521 ttttagctgt tgagttcagg ctagctaata acccactttg ttccgcattg taacttggat
     8581 ttgctcctgg cgcaagtggt tttccagctt cacggccatc aggagtattt cctgtatttt
     8641 taccgtaaac aacatttgaa gtaatcgtca ataatgaagt tgttggtttt gagttgcggt
     8701 aagttttatg acgtttgatt tgtcccatga agtattccaa gatccatgaa actaattcat
     8761 ctgcacggtc atcatcattt ccgtatttag ggaagtcgcc atccacttca aaatcaactg
     8821 ctattccttt ttcattacgg attgctttta cttttgcata tttgatcgct gacaagctgt
     8881 ctgccgcatg agagatccca gcaatacctg tagcaaatgt gcgttgcaag tcagaatcca
     8941 tcaaagccag ctgagctgct tcataagaat atttatcatg catataatga atgatgttca
     9001 acgtattgac atatagttct gctaaccaat ccatcatatt catgaagtta tgtttcactt
     9061 cttcaaagtc tagtatttct gatgtgattg gacgatattc tggtccaact tgggcgccag
     9121 tcacttcatc gactccgcca ttgatggcat ataatagtgt tttagccaag ttagcacgag
     9181 cgccaaagaa ttgcatgtct tttccgacaa ctgttcctga tacacagcaa gcaatcgcac
     9241 aatcatcaga accccattgt tcacgaagct gattatcatt ttcgaattga atcgaagaac
     9301 tttcgatagc gattctgcta gcatatgttc tgaatccttc tggaaggtgt tcagaataca
     9361 aaacagtcaa gtttggttct ggtgaaggtc ccatatttgt taatgtatga agcagacgga
     9421 aatcattctt tgtaactaat gaacgaccat ctaatcccat acctgcaatg gataaagtag
     9481 cccagatagg gaatcctgag aataattggt tatattcagg tgtacgcgcg aatttgacca
     9541 tacgtaattt cattactaag tggtcgatca attcttgtgc ttctttttct gtgattactc
     9601 cattatccaa atcacgttgg atatagatat caaggaatgc tgagatacgt ccgattgaca
     9661 tcgcagcacc attttgtgat ttgattgctg ctaaatatcc gaagtatagc cattggacag
     9721 cttcttttgc atttgcagct ggtttagaaa tatcatagcc atatttcgca gccatttctt
     9781 tcatttgcgc taatgcacga tattgttcag tgatttcttc acgtaaacga atcacttctt
     9841 cactcattgt atgataacca gtcatatcat gatctttttt cttttgttcc atcaaatagt
     9901 cgattccata caatgccaca cgacgatagt caccgatgat acgtccgcga ccgtaagcgt
     9961 ctggaagccc tgtaatgatt ttgtttttac gagcagcacg catttcagca gtataagcgt
    10021 caaatacacc ttgattatgt gttttgcgcc agtcagtgaa aatatgtgtc aattcatcgt
    10081 ttggtgtata gccattgctt tttagcgcat tgttagccat attgattccg ccgaacggca
    10141 tgaatgcttg tttcaaaggt ttgtctgtct gtagaccgac gattttttcc aaatctttat
    10201 ccaaatagcc tgcatcgtgt gaagtaatag ttgatacgat ttctgtgtcc atatcataga
    10261 caccgttacg ctcgaattgg atatcattta acttttgcaa ttcatcccat aatttttctg
    10321 ttgcttctgt tgggccttcc aaaaagctgt catcaccgcg atattcagtg tagttatttt
    10381 gaataaagtc tctagtgtct acgccttctt tccatttggt acctttaaat ccattccatt
    10441 gctccattgt atggacctcc taaatttctg ttttatgtaa cagtttttac gtgattagta
    10501 taacacagca ccctatcttt gcaagggttt tcttgttttt tatttaacaa tttttatgtt
    10561 ttatttacat atatttaaat caacgttgac ataccaacat ttaagcattg tgaacacttt
    10621 catctttttt tgcaaacgta taaggattta ttaaaaatga atgaaaaaga gacgccacct
    10681 gtattaatac agatggcgtc tcttcattaa aatactacaa cagttacgac tcgatgaaaa
    10741 tctcaggagc atcgataact tcgatgactt ctccaccttt tttctcatcc atcgcaaatg
    10801 atccattcga tgttcgatca cctataggga aatcagttgg gtcaatcgtt aactgctgcc
    10861 ctttttgatt catcaaggtt atttcttttg atcttccttc cgacatatag ataatacggt
    10921 gcggattttt tttcagctca cgcatgaccg tcactccccg cttcgcacgt gaagtttggc
    10981 tgatttcttg agcaagcatg cgtttaacag ctcctcgctg agtaacgatt acgatcggcg
    11041 tatcaccttc tgctaacaca agcagcccgt tgataatcct atctccatct ttcaaattca
    11101 tagacttcac tccagctgcc tttgctccaa caacgggaac atctgttaaa ggatagcgca
    11161 aacccattcc ttgatgcgtt actaagaaaa catctaagcc tggctgatct tctttcagat
    11221 acacagcaat cagttcatct tcttccgcct ttagcttcat acagctgagc ggacggctct
    11281 tataggtgcg ccatggttcg aactctgtca ttcgtgtttg cttgatcaat ccattcttgc
    11341 tgatgaatac aaatgttttc tctgcttgaa ttttttgata tgggaaaaca gctaaaatcg
    11401 attcgtctac agacagattg gagattgttt gagaaatgtg ctcaccagca tctttccatt
    11461 tcaaatcggg tagttcatgg acagggcggt agatcacatt tgctttattt gtgatcagaa
    11521 gaacatggtc caatgtactc aattcacctg agtatagcaa gtagtcgcct tctcgcatcc
    11581 cgatttcttc tggtttggaa gcactataag aacgaaggct tgttcgcttc acatagcctt
    11641 ctcgcgtcac actgacaatt acgtcttctt gtgccactaa gacttgcgta tcaattttga
    11701 tttcttcgat ttcgtcttcg attgtcgtta gacgagcgga agaataattt tttttcactt
    11761 ctcgcaattc ttttttcata acagagaaaa gctctttgtc atttgaaagg attttattca
    11821 actctgtgat ttgcgcaatc aagctttcgg attccttttg aagctctgtg atatccgtgt
    11881 tcgtcaaacg gtataattgc aatgtgacaa ttgcttctgc ttgttcttct gtaaattgaa
    11941 agacgtcgac taaattcttt ttggcgtctt ttttgtcttt actttcacga atcgttgcga
    12001 ttacttcatc cagaatcgat aaagctttca tcaatccttc tacgatatgc tgtctttttt
    12061 gagctttggc taaatcgaat tgactacgtt ttgttatgac ttgctttcga tgctcgatat
    12121 aacttcttaa aatgtctttc agtccaactt gatgcggagt catatggtcg atagcgacca
    12181 tattgaaatt gtaattgatc tgtagctcag tgtttttgaa caaataattc aaaattcctt
    12241 gagcattggc gtcttttttc aattcgacga caatttgcag acctgttcta tcactttcat
    12301 ctcgtacttc agctattcca tcgattttct tattcaaacg gatctcatcc atttttttca
    12361 ccaaggtcgc tttatttact tcatatggga tttctgtgat cacgatttgc tgtttattac
    12421 ccttcattgc ctctatcttt gttttcgaac gaagaataac ttttcctcgt cctgtttcat
    12481 aagctttttt gatttcatct tttccttgca agatccctcc tgttggaaag tcaggtccag
    12541 gaatatattc catcaatttt tcaaggctcg cttgtggatg atcgatcata tagattgtgc
    12601 catcgatcac ttcagctaag ttatgagtcg ggatttctgt tgcatatcca gcagatatac
    12661 ctgtcgatcc attgacgaga aggtttgggt atcgagctgg aaggactgtt ggttcttttt
    12721 ctgtatcatc aaaattccac acgagatcga ctgtttcttt ctcgatatct gctagcatct
    12781 ctccacttaa cttggataat ctggcttcag tataacgcat cgcagcaggt ggatcaccgt
    12841 ccatactccc gttattcccg tgcatttcga tcagtacttc ccgcaacttc cagtcttggc
    12901 tcatgcggac catcgcttca taaatactga tgtcaccatg aggatgataa ttccccatga
    12961 tatttccgac agattttgct gatttacgga atcctttgtc atacgtattg ccatctttgt
    13021 tcatggcaaa taatatccgg cgttgcactg gcttcaatcc atcacggata tcaggcagtg
    13081 cacgttcttg gataatatat ttggaataac gtccgaagcg gtcacccatc acttcttcta
    13141 aagttaattc ttgtacttct tgtcgatttt ccatcaggtg acctcctatt ctacttcaaa
    13201 taactgattg ttttcatttt tatctgctcg ttcttcatct aataaatcat tagaaactga
    13261 aggagagatt tcagtatcct cttttttatc taagatactg ccatcttctt cgagcgtaaa
    13321 ttgcacgtga ttttcgatcc attttcttcg aggttctact ttgtcaccca tcagtgtggt
    13381 tactcgacgt tcagcttgcg cagcatcatc gatacggacg cggatcaacg tacgggaagt
    13441 cggatccatc gttgtttccc acaattgttc cgcattcatc tctccaagtc ctttatatcg
    13501 ctgcagcata tatcctttgc cgactttttt aatcattgct gccaattcgt catctgtcca
    13561 cgcatattca atgacttgct tctttccttg tcctttggat actttatata aaggcggtaa
    13621 cgcgatatat actttcccag cttcaatcag cggtttcata taacgataaa agaaagtaag
    13681 cagtaatact tgaatatggg cgccgtctgt atccgcatcg gtcatgatga ttactttgtc
    13741 gtaattacag tcttcgatag aaaactcagg accgactcct gcaccaatcg tatagatcat
    13801 cgtattgatt tcttcatttt tcaagatatc ttgcatttta gctttttcgg tattgatgac
    13861 ttttcctcgt aaaggtagaa ttgcttggaa ctttctgtct cttccttgtt tagcagatcc
    13921 gccagcagaa tccccttcga ctaaatacaa ttcatttttc ttagggtttc gggattgtgc
    13981 gggtgtcaat tttcctgaaa gaagagattc gcctttttta cgcttcttcc catttcggct
    14041 ttcttctctg gctttacgtg ctgcttctct cgcttctctg gctttgattg ctttacggac
    14101 caacatctgg ctcatttcgc tattttcctg caaataaaag cccatttgtt cactgatgac
    14161 gttatctact acggtacgag caactggcgt accaagtttt tcttttgttt gcccttcaaa
    14221 ttgcagaaga ttttcaggta cacggataga aagtacagca gctaaccctt ctctgaaatc
    14281 actaccttcc agattcttgt cccgttcttt cagcaaacca acttttctcg catattcatt
    14341 ataagatttc gtcatcgccg ctttcatacc agcttcatgg gttcccccat cttttgtccg
    14401 aacgttattg acaaaagata aaacattttc tgaataaccg tcattgtatt gataagcgac
    14461 ctctacttcg ataccttctt tttctcctga aaaataaaca actggtgtca atgtatcttt
    14521 ttcttcattc agataatcaa caaattcttt gattccttct tcgtaatgaa aaatttcctt
    14581 gactggttct tctccacgaa gatcagaaag agagatttta acgcctttta gaagaaaggc
    14641 agattctctt aaccgttcgg ctaaaatttc atatgaaaat ttcgttgtac tgaatattgt
    14701 atcatctggt aaaaaatgga cagtcgtccc gtttgctttt tttgtttttc ctatcttttt
    14761 caaagtaccg tcaggtttac cgccgttttt aaatttctgc tgatactcta cgccatctct
    14821 aacgatcgtt acaattagcc atttcgataa ggcatttacc acactggctc ctacaccgtg
    14881 cagtccacca gaagttttgt accctccttg accaaacttt ccgccagcat gcagcacagt
    14941 aaaaatcact tcaactgttg gtattccgga agcatgcatt cctacaggca tcccacgtcc
    15001 gctatctgca acagttatac tattgtcttc atggatggtc acatcaatct cactgccata
    15061 acctgataat gcttcgtcaa ctgcattatc gacgatttca tagactaaat gatgcaagcc
    15121 tcggctgtca gtggatccaa tgtacatccc tggtcttttt ctaactgcct ctaatccttc
    15181 caatacttgg atggacgagt cattgtattc gttgtttact ttttttgcca aggcaaacgc
    15241 tccttcaata atactttgcg gcaaaaccgc accacgatta tcatactaga aaaggtcgtg
    15301 aaaaaaaaga gggtagacga gaaatcaatt ttaattatct cctaaccgct ttttcgacat
    15361 tcccctctat cgatcatctc ttctttgtgg tcatttttat ttttggtttt ctttcaactt
    15421 accaagttca atcttaatac aacgatccat tacgatatct gtacgtcctg cttttcttag
    15481 catctcagct gcttctttgt tttcaatccc taattgtgcc cagaacactt tagcatcagt
    15541 tttcaaaaaa tcacctgcta tttcaggtaa aaattcactc cgtctaaata catctacgat
    15601 atcgattgga tcatcgattt ctgtcaaatt cgcatagact ttctcgccta gaatttcttc
    15661 accagccaat aatggattta ctggtatgat tttatatcca ttgtcttgca atacctgtgc
    15721 aaccatgtaa cttgttcgtt ctggtttatt gctcaagcct acgacggcaa tattttttgc
    15781 ttcttccaaa tagttgaaaa tcgtttcttg tgttggattt tttacagaca tttctgccac
    15841 cccttgcttt tctttttatt ccatctatta tacttgtatc tttcattttt tcaattgttc
    15901 ctgttaccta ttcatgctaa aataaaggat agttttcaac ttgaaagagg gtaaaaaatg
    15961 aaattgatct tattacttat tttagcatat cttttaggtt ctattccttc cggtgtctgg
    16021 attggcaaat tgtttttcaa aaaagatatc cgacaacacg ggagcggtaa tactggaacg
    16081 accaatacat ttcgtgtatt aggcaaaaca gccgggattg ttgtcctgct tatggatatt
    16141 ttaaaaggta cattggctac ttgtttgccg atgatcttcc acctttctgg gattaaccct
    16201 ctttggtttg gggtatgcgc cattcttggc catacatttc cgatttttgc tgggttcaaa
    16261 ggaggaaaag ctgttgcgac aagtgcagga atgcttttag gatttaatcc tgtatttttt
    16321 gtttattctt gtatcatctt tatcgttagt ttattttgta caagtatggt gagtttgagc
    16381 agtatgatca gtgcagtatt gatcacttta tctacgatcg tccttcctta tgtagctcct
    16441 gtgattttgg ctaagcccaa ttggttactg acgatcattg cctttttagt ttcttccttt
    16501 atcttttatc ggcataaaga caatatcaag cgtatcagaa accatactga aagtcgaatc
    16561 ccgtttggtt tgaatagcag caagaaaaaa tcctgatact ttttcattcc aaagagataa
    16621 aacgggagtg tgacaaatcg atgtcacact cccttattac ttacaggata gcttttttac
    16681 acaccttggt aaacggtgtt ccctgctaac ttctcggtca caacctctta cttcactgtg
    16741 atggaatatt ttgtcttgaa gatttcatgg gcacctagtt gattgatccc tactttttcc
    16801 atcaagtttc cactactgtt caccgtgtca gcgattccgc accacggttc gatacaaacg
    16861 aaaggcgctt cttttggata aggagaccag atccctacat aaggcatatc tttatagctt
    16921 aaagttacgc tatgggaaga tttttcactt cgaatggcgt acgcatttag accccttgtt
    16981 tcaaaaacca aggcatcttg ctcaaaaagt tcacgattca aagccagatt cgtatttgtc
    17041 tgacctaacg tcttttgatc catatcgatg aatgggcctt tcaatggtag gcgtaatctt
    17101 gatttcaatg gagaaaatgc cagatagaaa tcttcaaatg acaatccttc ttcaagaggt
    17161 acattgaaag caggatgtcc accgatagaa aagaacattt ctttttcacc ggtatttgcc
    17221 acttgataat gaaccgtgat gccgtctcct cctaattcat agctgatcac taattcaaaa
    17281 tcaaatggat acttttctaa tgtctccggc gtactcctta agatgaaact agccatttct
    17341 tgtccatgtt caatgacttc aaaatccata tcccgagcaa atccgtgttg ccccatttga
    17401 tatgttttcc cttgataaac atactgatca tcttttaaac gtccaacaaa agggaataaa
    17461 accggtgcat gtctgcccca atacgctgga tcaccttgcc agatatactc taaaccatgt
    17521 tcttttgatt tcagactgat caattccgca ccgtgttcag cgatttcggc gatcagctgg
    17581 tcattttgaa taataacagt cattgccatt cctccaactt cgacttactt tttaaaatgg
    17641 gaaggctgga acaaacattt ctatctgccc cagtcccctt acttctaata actgtgtttt
    17701 gctaactcgt ctttaaaacg ggaattcaat acttttaaat aagttccctt catgcctaac
    17761 gaacgagatt caatgattcc agctgattcc aattttcgta aagcattgac gatcacagaa
    17821 cgagtgatgc cgatactatc ggcaatatta gaagcagtca gacgaccttc atctccatcc
    17881 aatgcttcaa agatagcttg tacagccttc aattcgctat aagataatgt gttgattgcc
    17941 atttggacag ccgttgcact ccgtacatct gcttcgatgc ttcgagactt ctgataaagg
    18001 atctgcatac cgacaaccgt agctccatat tctgctaaga ctagatcgtc atctgcaaac
    18061 gattgttcca tacgagataa gataatcgta cccaaacgtt cgccagctcc aaagatcgga
    18121 acgacagtcg tcagaccaaa tggatatttc tctcttgact ctaccggaaa tgcagtaata
    18181 tcactagtaa ttgggatatt agcttcagtt ttcatcaact gtgccactgt atcagtataa
    18241 gattcaggaa attgtttctc aacaaacatt gttttgattc gttcattgtt cacatcgtga
    18301 cgttcattat aacctagtaa tactccgtca cttcctatga tataggtgtt gctgtcaaga
    18361 ataccaccta gtgtgacagc cattttatcg taaggcaaat ctgcttgcat gtcaaaagta
    18421 ttcttttgct gcaaaagtgt gtttatcttg cgggttttcg ttagcaagtc tgtcattgtc
    18481 gtcctccgat tttttataaa atatagcgac ttaaatcttc atttgccgca attgagccaa
    18541 gtttgtcatc cacatatgct tctgtgatcg ttatttctcc catttgcata tctggtgctt
    18601 caaataatag atcttctagt agtttttcca aaatggtatg cagtctgcgt gcgccgatat
    18661 tatctgtgtc acggttgaca tcgaaagcga tttgagcgat ccgttcaatt gcttcttttg
    18721 taaacgtaac tgacacattt tctgtaccga tcaacgcgac gtattgtttt actaaagcat
    18781 tatttggttc ggtcagaatc ttcacaaaat cttcagctgt caaatcatct aattctaccc
    18841 gtatcgggaa gcgtccttga agttctggaa ttagatcact tggtttagac aaatggaaag
    18901 ctcctgaagc aatgaatagg acgtgatctg tttggatgat cccgtatttt gtgtttactt
    18961 gtgaaccttc gacaattggc aatatgtctc tttggacgcc ttctctggat acttctccag
    19021 attgctgtga tttagaagtg atcttatcga tttcatcgat aaaaataatt ccactttctt
    19081 ccgctaattt gattgcctca ctatggatat cagcatcctt gactaatttt gctgattctt
    19141 ccttgatcaa taactctaaa gcttctttga ctgttaccgt ccgttccact tttttcttcg
    19201 gtgtcaaagc acctagagtt tcatttaggt cgatccccat ttgctccaat ccattgttca
    19261 tcattggtgc aggtttcttt tggtcttcaa cttcaatggt tacttcacga ctatccagta
    19321 aacccttttg tagttgttcc aagactgtac ggcgattgat tcgaatatct tctgtcactt
    19381 cttcttgcgg ttcagcggta ttttgctgac tagcattaaa aagattcatc atttgttcaa
    19441 attgattagt ggattgtttc tgttcttttt tgataccagg taccaagatt tttaccaaac
    19501 gtttttctgc atttttttta gcacttgtgt atacacggct gtactgctct ttttctacga
    19561 tttggatcgc attttctact aaatcccgaa ccattgattc tacatctctt ccgacataac
    19621 cgacttcagt aaattttgtt gcttcgactt tgacaaatgg tgcattgacg atttttgcca
    19681 aacgtcgtgc gatttctgtt ttaccgaccc ctgtaggtcc gatcatcagc atattttttg
    19741 gtgtaatatc ttgttgcatt ttttcatcta actgcaaacg gcgatagcgg ttacgcaaag
    19801 caacggcaac agattttttt gctgcgtgct gtcctacgat atattcatct aattcactta
    19861 cgatttgtct tggtgtttgt tttaattcat acatatctac tcacctctta cagttcttct
    19921 acgataatat tatgattagt aaagatacag atatctgcgg caatatttaa cgcattttct
    19981 gcaatttctt tcgcactcat gtcgttttga gcatagtttt tcattgctcg ggctgcagcc
    20041 aacgcaaaat tccctcctga tccgatagct aagataccat catctggtgt gataacttct
    20101 cctgtacctg aaacaagaag catttcttta tcattcatta cgatcagcat cgcttcaagt
    20161 ttttgcatag attgctgtgt ccgccattct tgagctaatt cgacagctgc gcgttgtaga
    20221 ttcccgttat attcgtttag tttgccttca aatttttctt ctaaagtaaa cgcatctgca
    20281 acacttccgg cgaaacctac aacaacttct ccattgtaga tacgtcgtac tttctttgca
    20341 gtgcctttca tgacaacaga ttcacccatt gttacttggc catctccagc catagcaaat
    20401 ttcccgtctt tttcgacggc acagatagtg gttgaatgaa atggtgattc gaccatgttt
    20461 gtttcctcct aaatttgttc ttttccttat ttttacgctc ttggatggaa agaacgatag
    20521 tttttttgta aactttcttt tgttacatgg gcatagatct gggtagtcga taaattggca
    20581 tgtcctagca attcttggac tgtccgcaaa tctgccccat tatttaataa atgagtagca
    20641 aatgtatggc gcagcatgtg aggatggata tcactatcca aactgctttt tttgatcatt
    20701 tgattcaaga catattcaat tccagtaggc gtgattggtt ttccatgatg attcacaaaa
    20761 acgatatcat gctcttcgtg atattttgtc atcaatacct ggcggccttc cctgaaatat
    20821 tcttgcagag catcttgagc aaaagaacct agaggagcat atctctcttt gttcccttta
    20881 ccatggatca acatgacctc tgaatcaaaa tcaatgtctg ataccgacaa actgctacac
    20941 tcacttaaac ggatgcctga cccgtaaagg acttccagta aagcgcggtt cctcaaatcc
    21001 aaaggactat ctcctgctac gctgtcaaaa agtgcttgca tttcattttc ataaaaaaaa
    21061 cgtggcagtc tcaaattttt ctttttgaga tgaacgtaag aaaatggatt ttcttttatc
    21121 acttcttctt tgagaagata ctgataaaac gaacgtaggc tggctatctt gcgactgata
    21181 gaatttctgc tatagctagg acgatgatca gctaatgcac tcaaatagac acgaaccgta
    21241 aaatattccg ccttcagcag atcagcgtct ccagattcct ccaaaaaatg tttgaaatgg
    21301 agaatgtctt cttcatatgc ttctttcgtt ttttctgaat atcctctttc gacaatcaaa
    21361 taacgcaaaa atttttgttc ccacgtggct tctgtcattt tctcaccttc cttttgtccc
    21421 agagaataat ttagcacagc gtttataaaa agacaaataa attgtcagaa tttaaccaat
    21481 atttctaagt ttgttcacaa ttggcgcaat tcctcacatt ttgtttgaga aattcttaag
    21541 aaaaacgcaa gaaaaagagc gagactatcc gtctcaccct cttcatggag tttccttact
    21601 catttgggat tgcagttttt tctaattctg tttttattgc ctcattagct tttaaagctc
    21661 gttgtgcaat tgcttcatat cttgcttttt tgtctcgaat gcgctctggc agttcaggaa
    21721 acagaccata gttggcattc atcggttgga aatgttttcc ttccgcatga gtaatgtagt
    21781 aagccatact tccaatagct gtttcttgtg gaaactcgac tggttccatt tcttttgcta
    21841 agcgtgcggc gttgattcca gcaagtagtc cgctcgctgc actttcaaca tagccttcta
    21901 caccagtcat ctgacctgca aagaacagat cttcgcgttt cgttgattgg taagtcggtt
    21961 tcagcaattc tggcgaattc ataaagctgt ttcggtgcat cacaccatag cgaacgaatt
    22021 cagcattttc taacccagga atcatccgga aaacacgttt ttgctcgccc catttcaagt
    22081 gagtctgaaa gcccacgatg ttgtataaag aagctgcagc attgtcttga cgcagctgga
    22141 taacagcata aggacgtttg ccagttttag gatcttccaa tccaactggt ttcataggtc
    22201 cgaaaagcat cgttttgaat cctcgctgtg ccattacttc aattggcata catccttcaa
    22261 aatatttttc tttctcaaaa gatttaagtt ctgccacttc ggcattgatt agtgcctcat
    22321 ggaaagcttt gaattcctct tctgtcatcg gacagttcaa gtaagctgct tctcctttgt
    22381 catagcggga cttcaaatat actttatcca tatcgatcgt agctttgtct acgattggtg
    22441 cagctgcatc atagaaataa aaaccatctg aaccattgaa tgctttgatg ctttctgcta
    22501 atggatgaga agtcaaaggt cctgttgcta ctacggtgat tccttccggt atttcagtaa
    22561 tttcttcgtt tatcactgtt actaatggat gctctttgac ttttcgggta attgtttcag
    22621 aaaaagaatc gcggtcaaca gctaaggcac caccagctgg cactgctgtt tcatctgccg
    22681 aagtgattac taccgaattc aatcggcgca tttcttcttt caataaccct actgcattag
    22741 ccaaactgtt tcctcttaat gaattggaac atactagttc agcaaaatta cttgtgtgat
    22801 gcgcctcagt cgatttgact ggacgcattt catataaacg gacagggact cctgcttgtg
    22861 cgatctgcca agcagcttca cttccggcaa gccctgctcc aacaacattt accattttca
    22921 atgaaagaac ctcttttcta ttatttttgg atgtcttctt cgtaatctcc atttacacag
    22981 actacttgtt taccgccttt gactttcttc tcgaccagat atccgtcaca ttttggacaa
    23041 tttctgccca ctggtttatc ccaagaagtg aattcacatt ctgggaaacg ggaacaacca
    23101 tagaatagtc tgtttttctt agattttcgt tcaatgactt gtccttcatg acatactggg
    23161 caagtcacac cgatttcttt tacgattggt ttcgtattcc gacaatcagg aaaattactg
    23221 caagcataaa acttcccata tttccctaat tttatcacca tcggatgtcc acagacatca
    23281 caatcgaatc ctgctggttc atctttgatt tgaatttttt cgattttttc ttccgcattc
    23341 gtcagttctt tttcaaacgg tttatagaaa cgatcaacca ctttgaccca cgcttccgtg
    23401 ccttcttcaa ttttatccaa atctccttcc atttctgctg tgaaatggat atcgacgatt
    23461 tgagggaaga attcacagat taatgaattg acaatttctc ctagttcagt cggctcaaat
    23521 cttttttgtg tcaattttac atagtagcgt ctttggatcg tatcaagtgt tggtgcataa
    23581 gtggaaggtc gccctacccc gttttcttct agtgcacgaa tcaacgtagc ctcactaaaa
    23641 cgtgctggtg gttgagtaaa atgttgtttt ggttcaatat caacagattg gactacatcg
    23701 ccttcttcaa gttctggaag aatattttct ttctcttctt tgccgtcatc tctaccttcc
    23761 acatacactt gcatgaatcc tttgaatttg atttttgaac cgttcgcaat gaagatgaca
    23821 ccattttgtt ctaatgtcac tttcattgta tctaaaatag ctggtgtcat ctgactagcc
    23881 acaaaacgtg accagatcaa tgtataaagt ttcaattgat ctttatctaa atattgtttg
    23941 agttcatctg gtgtacgaag cacactcgtt ggacgcacag cttcatgggc atcttgtgcg
    24001 ccttgagcat ttttgatctt tcggtgatca tgtctagaaa attcttttcc atacgtatct
    24061 tcaataaatg ctgcggcctc tgcttttgct gtatcggcaa tccgggtaga atcagtacgc
    24121 atgtatgtaa tcaagccgac tgttccttgt ttacccaaag tgattccttc atataattgc
    24181 tgtgctacca tcatggtttt tcttgttcga aagtttaact tacgggcagc ttcttgttgc
    24241 aggctacttg tagtgaaagg attcgcaggg tttcttttgc gctctttctt ttctactttt
    24301 ttaacttcga agtctgttcc atcaattcta gcagtgactt cttttacact ttgtgcatct
    24361 ggtaatttct ttttcttacc gtcgattccc cagaaatttg ctttgaattt cttgcgagct
    24421 tttttaaaat taccgtcgat gctccaatat tcttctggaa caaattctcg gatctctttt
    24481 tcacggtcaa taataatttt taaagcgaca gattggacac gtcctgcact taaccctttc
    24541 ttgaccttac gccagagaat aggactgatc gaataaccaa cgattctgtc taaggtacga
    24601 cgagcttgct gtgcatctac taaatctaca tcgatcgttc ttggttcctt aaaagctgct
    24661 ttgactgcgt cttttgtgat ttcattgaag accacccgat ttttttcttt cagatccaac
    24721 ccaagtaggt acgctaaatg ccaagcaatc gcttctcctt ctctatccgg gtcacttgcc
    24781 aagtaaactt tttgagcttt ttttgctgcg gctttcaggc ttttgatgac atcgcctttt
    24841 ccgcgtatag aaatataatg gggttcataa ttgttttcaa aatcgatacc cattttactt
    24901 ttcggtaaat ctcttatatg gccaacactg gccattactt tatagtttcg tccaagatat
    24961 ttctcaattg tcttggcttt tgcaggtgat tctacaatca ctaaatattt atacgccatt
    25021 actgaatgga ctcctttcca ttgtcgtgtt cctactatta aaagaggatt catacaaatc
    25081 gtagctaatc atatccattc agaaaacgtt tgtcaagaaa agcaaatgaa aagtatggtg
    25141 ttattcttta aattctgcca agatatcttc acccgaaacc gtgcaaactg caccgtcttg
    25201 gatcaaatga tgacatccgg tagaatgttc tgttaaaata tttcctggaa cagaaaaaac
    25261 tgttcttcca agatccaatg cttgctgtgc tgttatcaga gagccgcttt ttttcttggc
    25321 ttcaaagaca catgttcctt tagacaaagc tgcgataatc cgatttctca ttggaaaaca
    25381 atgctttttc gggccggatt gaggaggata ttcactcaaa agaagatgat catttgcaat
    25441 cacctcttgc aagtcacgat tttcagcagg atagacttgc tctatccctg ttccaatgac
    25501 tgcgattgtc cgtccaccat gagagattgc tgcttggtga ccgtatgtat ctatcccttt
    25561 cgcgcagcca ctgacgatca catatccagc ttctatcact ggttttagta aataataaac
    25621 tgtttttttg gctaaagcag aagtgtgtct tccaccaaca acgcttaaca tgttacaaga
    25681 aagtaaagat agatcaccat tataaaacag tacaattggc ggataggtca agtgaagcag
    25741 ctgaggggga tatttgggat caaagcaagt gataaacgac tgtttcgatt ggattttgcc
    25801 ccagttagcg tgaatttcct gccaactttt gcgaaacagc tcccggtgtt tggttatttt
    25861 acctatctta atgatgtctt caaatccgat ttctttataa ggatactgca tcatccagtt
    25921 agcaagtgcc cattttcctt gactactgat tccttttgta taagcaagtt tcaataaatt
    25981 ttcttctatt tgatacatca taaaaccgcc tttcgtctct ctttctaata agatacgtaa
    26041 aagcggctgt tttttattgt aaatattttt ttacaggtgc aaaactttta cgatggattg
    26101 gcgtaattcc ttgtcgttca agtccttcta agtgttcctt tgttccataa cctgcattat
    26161 gcgagaaacc atatccagga taaagcacgt catattcttt catcaattgg tctcgatata
    26221 ctttagcaat gatacttgca gcagcaatcg acacagatcg tgcatcccct ttgattattt
    26281 tttcctgctg aataggcaaa tccaaggtca tcgcatcgat caacaaatag cttggtacaa
    26341 tggacaattt ttctactgct tggatcatcg ctaatttact tgcttcataa atattcacat
    26401 catcgattgt ctgctgatca acgatcccga tcccgattgc cagggctttt tcttgaactt
    26461 gttgataaag aagttctcgc tttttctctg ataattgttt ggagtcattc agccctaata
    26521 tgatctcatt tttcggcaag ataactgcag ctgccacgac aggtccagct aatggtcctc
    26581 ttcctacttc atcaatgccg gcaatccatt ggtgaccttg tgcgtaccct tgcttttcaa
    26641 agcaaaacat ttcttcatat ttttccttaa ttaatgcttc ctttttcaat cgtcgttcaa
    26701 aagaagccag tgcttgctgc acgcctttgc gtggatcatt tcttaatgat tctagtgtag
    26761 gatcatctat tctctgtatg cttttcaatc gttctttgat actttggatg gattcagtca
    26821 tcactttttt gctccatttc ttcgcaacga tccagtgtgt atcgtccaag ttttccttga
    26881 cgaatatcat gcacgactaa ttcgctgcca cgatcatagt cttctttata cccacgtttt
    26941 gctgtaatat ccatcagtaa ttccggtgcc aatttttctt cttgctcgat tgataaacca
    27001 taacgatcgc tgattcttcc tgaataatat cgggcaaaaa agtctaatcc gtaaatcgct
    27061 aaatcatcta aatggacaag ctggtctttg atcgcgcctg tcagcgctag ttttttgccg
    27121 atttcttcat cttcaaattt gggccataaa atccctggtg tgtctaacaa ttccaactca
    27181 ttacctaaac gaagccactg ctgacctttt gttacgccag gcttatttcc tgtctgtgca
    27241 attttcttgc ctactagtcg attcatcaac gtagatttcc caacatttgg aatacctaag
    27301 accattgccc gaatagcgcg aggcttgatc ccccgagcag catctcttgc ccgtttttct
    27361 ttcaatgctt ctttagccgc aggtactaga cctttaattc ctttactttc ttgtgccgtg
    27421 atagctaatg caagatggcc ttgtttacga aaatattctt gccacagctt tgtctgttgt
    27481 ggatctgcca gatccgcttt attcaataaa acaaggcgag gtttttgctg aatgatctga
    27541 tctagcattg gattacgaga agataaaggt aatcgtgcgt ctaccaattc aaatacgatg
    27601 tccacatatt tcattttttc agaaacttct cgtcgggctt ttgccatatg tcccggaaac
    27661 cattggatgg tcattgttct ctccattcct ttcataccgg aatattcccc ggctttactt
    27721 gtttttttct ttttcaatat aaattcactt ttttattata aagaaattcc atctattagt
    27781 taagtgaaag tgaaccagtc ctcattctat cacatcttca cttttttgat gatgcaaatt
    27841 tttattttgg tttctatata tgaatctaaa tttttgacag cgtttcctaa ataagagatg
    27901 atagacgaac aaaacagaaa ggagacgaaa caaatgaata gtatggtttt tgttaatttt
    27961 cctgttgctg atgtcaatcg ttccgttgct ttttatgaaa agctgggttt taaaaaaaat
    28021 gaagaatttt ctactgaaga agctggttca atggtatggg atgacacttt ttggataatg
    28081 cttctctctc atgattttta taaaaaattt ataaaagata aagaaatagc tgataataaa
    28141 aaaacaagtg gtgctttgat ttcgtttagt gttgaaaatg cggatgaagt aaaaaaattt
    28201 gctcaaattg caaaagaaaa tggtggtact ttccacgaag tagatatggg aatgcctgaa
    28261 gaagaaatgt tttcattaga agtccaagat ctagacggaa atacattaga acccgtatgg
    28321 atgaagatgt aagcagataa ctaatctgac gttttttata acaaaaaaac aaaggcggat
    28381 gatacgataa gtattctccg cctctatttt ttttattcgt acaatctctt ttttacagtt
    28441 tataaaagca gtcttctcac ccttttttta attgttagat gccagtgtat gtaaatcacc
    28501 aatgtgtcat gacacattgg tgattttatg gtatcctttt attattaact gacaaaggag
    28561 agaatatttt gaacactttg attgcaaaag acgtttttag attgcccgaa atgaatgcaa
    28621 gattgttgtc gacctctgct cttttattag cccttgatgt catcttatac aagatagcga
    28681 ttggtcctgc ttattttcaa atcacattcg gatttttaag tatggcgctt atgggttatc
    28741 tatatggacc gatctgggct ggtttgcttg aaatgttgtc gaacacttta aatttcacga
    28801 tatttggttc aggaacgttt caagctgctt ttcttatcac ggctttcctt ggtggattta
    28861 ttaatggctt atttttatat cataaaaaga taaacctgcc ttccgtcatc gctgcccaat
    28921 tattgattat gatcccagtc agccttttta tcaattcttg gctgatgtct gttttgtttg
    28981 gtgcagattt caaagtaatt tttctaggta gattcatcag gaatatgatc caaatcccta
    29041 ttcaaataat ggttgtttat tcatttatca aaactttaga ggctagaaaa gtgtttaaac
    29101 gagctgtttg attttacgta ctaaggtagt aaactgattt tggattaaac aataaagcgt
    29161 gcagtctcaa cagactgcac gctttattgt ttctcactgg atccatttta tttcatcaag
    29221 tggataatag acaaatcttg ctttacctag aatatcagtt ttattgatcg tgccaaacga
    29281 acgactatct tttgaaactc ttcgattatc tccaagtaca aaataattat cttccggaag
    29341 cttggattgt cccgttagct cttgtagtgt aaagtccgtc gtataggatg catttgcatg
    29401 atctgatttc aggtttttag ttagataagg ttccttaact actttcccat tgatcttcaa
    29461 ttgatcattt tggtagctga cattttctcc tggaagaccg attactcgtt tgatataaat
    29521 cgtgccatct gctaattgaa aaacaacaat atcaaatcgt ctgatctccg aaaatttttc
    29581 catcacaacc atatcacctt gcttcaacac gttttccatt gaattgcctt ccactggtac
    29641 cgggatgaat aaaaatcccc gcaacatgaa cgcaaggaat acagaaacaa gtaagtattt
    29701 gaatacaagc cagaagcgat taatatagcg ttgttttttt gtcaatctct atcaatccat
    29761 ccctcttttt ttcattcttt ctttattata agaaaaatat caaaaaaatg cgactagata
    29821 gctgaaataa aaataattct ttcaattagg ctattggaca aaaaactttt ttgatcataa
    29881 agtacaaaac tatctcttat atgtcatgat tattttctaa atatttttcc catgcttcgt
    29941 caaagatatc catggacggc aaatagtcga cattcagttc cagataatta gaaatttctt
    30001 cgtagctaga gctgtgctta ggaaattgga tgtctttacc ggcttctgta gcgaaaacag
    30061 ataaagcatc atgtgtgcca ccgcctcgca gtgtcattaa ataatgaaaa aagcttcgat
    30121 tcacccttcg ttcctccaat tttcttcgat aaaggcagcc cggcgtgcat ggtttctttt
    30181 aaaattttca ggatgctttt tataatactc ttgatggtct tcttctgcta gataaaatgg
    30241 ttgggctggt tcgatttttg taacgattgg ctgttcgaaa cgtccacttt cagctaactg
    30301 tttcttactt ttttcagcaa tgatccgctg ttcttctgtc aaataaaaga tcaccgggcg
    30361 ataattgtca ccgcgatcct cgaattgtcc gaaagcgtca gttggatcag tttgctgcca
    30421 gtaaagttct actaattgtt catatgaaat caatgttgga tcatagataa tctccactgc
    30481 ttctgtatgt ccagtcgttt ggctttttac ttgttcataa gtcggatctg gtacatgccc
    30541 tccagtatat cctgacatca ctgtatggat accaggaagc gtgtcgaaag gctggatcat
    30601 acaccagaag cagcctcctg caaatattgc tcgttccatt atgattcccc ctctaatact
    30661 actcatccga tgatttttta ttttcttttg tcttgctttc agtactgctg gtgctagttt
    30721 tgttggtatt tgagttgttt gaagcatcag aggtatcttt tggcaaatat agactaaacc
    30781 gaatgtcatc atttactaag tcgattttat ctgctttaat atacatcccg ttttgcattc
    30841 ggaattgatc caacctaagc atgatagtca gatcatctgt attgacttcg atccaatctg
    30901 gtaatttata gtttctcttg atcatattca tcacatcttt gattggcaaa ctcaatgttc
    30961 cgatagaaag acttctagct tttagttgga catttccgtt ttccatcaca tacggatcaa
    31021 aatataaata gaaatcgata ggaaaaccta gtacttcgaa agtaccattg agtagtgctt
    31081 cattttccaa ataaaattta tattcaatat cactatcttt ttgatactca ctaaggaaaa
    31141 agctgatgat ttgattgact ttttctttat ttgaattgat cgttaagaca ggatctcctt
    31201 gtctttccgt aagagctggt atttctggga gttctggttc ccgattagca aaaatacggc
    31261 tgcctaaaaa tacggtcgaa ccaagtacta ccccaagaag gataaggaag gcgaatttcc
    31321 agtaatttgt tttctttgtt ttgtttgaag atacgtcaga tcttgttttc atgtttattg
    31381 atcatcctcc ttctctaacc attggttttg tgtctcgatc agctgatctc tcactgcatt
    31441 tgccatcaat tgataaccta aattatttgg atggaaatga tcttcttcat agagcacatt
    31501 atttttgatg ccatttagat tttctgtcgt gctagttgtt tgatctgttc cttcttctgt
    31561 gacacctact tcctgattca atccttgata aagcaaatca ttgattggga taaagtagca
    31621 gttcggcatt ttttttgtca ccgtttctgt tcctttgttc caattgttaa caattgtctg
    31681 catgtcagtg atctcaggaa agttcaaata aaatggattg tagatgccta aaatgtaaat
    31741 aggtgcggtt ttattgaggg atcgaaattc ttccaatagt tctgtgacat tttcctgata
    31801 gtttttcagt ggctttttaa aggaattgac cgttaacccg aataaattat tttggataac
    31861 tttcatcaaa tcattaccgc caacagtcag tgtgataaaa tcagctgttt tgatattgtt
    31921 tcgaatttct tcttttgttt ttactcgctt taagatctgg tcacttcgtt caccatttac
    31981 accatagttt tctgtttcaa ccgcggtcaa attataacgg tcttttagat catttgcgac
    32041 aaggggaacg aatcctcctt gcttggtttg atctcctact ccttcagtca gcgaatctcc
    32101 gatagctatg tagtgaagtt tttctttttt atttggatct tctctcacag tagttactgg
    32161 tttcattagt gggccagctt tcggtataga agcagctaaa cctgcataga tgatgatcgc
    32221 aattaataat gggaacaaaa cgaggatcaa atgtcgtttc gttaatttca ttttttcacc
    32281 tcttgtattc gattggttct aatgttatca aaaattttat caaaaaggta gtctttatga
    32341 aaaaagaaaa caattatttt cctaatttta taaagaaaaa gagaataagc tgcaagcctt
    32401 tcaggctgag ggcttatcct cttttcgact aatctacata atacatgatt gcaaacgcgt
    32461 ctttaccagt atgcgtagcg ataaccggat tcgtatgcag tacagggata tgcatgtccg
    32521 ggaataattc ttgcaaaccc tctttgaatt cttgcgccaa ttcttttgca tccgcatgtg
    32581 aaataccgat ttgtcgcaca tttggcaatt ctttcagttc atttttcaaa ttattaaacc
    32641 atttatcgaa cgttttcttt cctcgcccct tagttacagg gattaattca gaatgggcga
    32701 aattcatcac cacgcgtatg ttcaaaacac tagacagcaa accagttgtt cggctgattc
    32761 ttccaccttt taccaagtta tctagtgtgg atattccgat gtataatttt gtattgtctt
    32821 gtactttttt gacttcttca aggattttct ctactgtttt accttctttt gctaattcag
    32881 ctgctcgaat tacctgaaaa gataatcctt ggtcagtggt atcactgtcg ataaccgtta
    32941 ctttcgtttt tgttagatta ctggcttgtc ttgctgcttc tactgttcca ctcaagcctt
    33001 ttgtcatatg gatcgaaatc acttcactgc cgtcttctcc tagctcatca tacatcgaga
    33061 tgaattctcc aattggtggt tggcttgttt tgggcagatt ttttgctttc gccatcattt
    33121 ccataaatct ttcgccgtct agttggtcat catcgggata tactgttccg tcgatcatga
    33181 tggatagcgg catgacacga atgcctaaag catcgcgtac atttttttcc atcgtacatg
    33241 aagaatctgt tacgattttt acatttgtca ttattttctc tctctctttc tttaacaaga
    33301 tgccttattc atgttaaact acatgtaaga caatttctat ttttagtata acagacaaat
    33361 aatgattttt cattaattac acctagtaaa ggagccgttt tcatggaaaa aacgaaattt
    33421 aatcgaacgt atgaaatatt aaatgaagtg tttaatgcag tcacccatgg aattgggact
    33481 ggattgagta tcgcgggact agtggttctt ttagtaaaag gtgcccactt gcattcctcc
    33541 ctccatattg tctcttatgc tatctatgga tcgatgatga ttcttttatt tttaacgtct
    33601 actttgtttc attcattgat ttttacaaaa gcaaaaaagg tgttccaagt attcgatcat
    33661 agttcgattt ttcttttgat tgcaggaagt tatactcctt tttgtttgct gagtatccga
    33721 ggatggctag gttggacttt atttgtatta atctggttgt ttgcgattct tggcattata
    33781 tataaagcgt tgacgttgca taaacgggag agagtatcaa aagtatctac gatcatctat
    33841 atttttatgg ggtggctctg tgtcgtggct gctgttcctc tttatcactc tcttggaatg
    33901 actggtgtta ttttgatggt agcaggcggg ctttcttaca cgataggcgc attcttttat
    33961 agcttgaatt ctatccgcta tatgcatgtt gtttggcatt tgtttgttat gttaggtgca
    34021 ggttttatgt acttctctgt tttattcttt acttaataaa aaaaataaag ctatggatgg
    34081 gacagctcta aataacagct agtctatcca tagctttttt atatcgtcaa ttcgcctgtc
    34141 ccgcttttta caggattctc attacgctga tacgtttcaa attgatgcgg ataacgattt
    34201 ttttcatcca tttctccttg actagtattg atcagtgacc aatcagacca gtcaacaggt
    34261 ggaaaataag catctccatc gaatacatga tggatgactg tacggtataa tatcgtacaa
    34321 aatggcagaa attcttgata aacagctgac cctccagcga tgaaaaagat accgtctgtt
    34381 tctttttcca gagcaagcac gtcttccaca ctatgcatga catgcacgcc ttctgcttta
    34441 taggaagaat ctctcgtcaa aacaatcgtc tgacgattcg gcaacggacg gctacccatc
    34501 ccttcgaatg tttttcgtcc catcactagt gtatttgctt ctgtcatttg tttgaagaat
    34561 ttcagatcat ttggcaaacg ccaaggaaga cggtcttctt ttccaatcaa tccgttttca
    34621 tcctgtgccc aaattgcagc taacattttc tcatcctttc tgattctaaa cagcgatagg
    34681 agctttgatc gccggatgtg ggtcatatcc ttctaattgg atatcttcca tctcaaaatc
    34741 aaatactgac tgcttttcct gattcaaaac taaagtcgga aatgcgcgta tttctcggct
    34801 caattgttct ttcatttgtt cgatatgatt gatataaaga tgagcatctc ctaatgtgtg
    34861 aacaaattcg ccaaccgcta aacctgtttc atgtgcaatt aggtgagtca gcaacgcata
    34921 acttgcaatg ttgaacggaa cgcctagaaa cacatcccca ctgcgttgat acaattggca
    34981 gcttagccgt ccctcttgaa cataaaattg aaacatcgta tgacaaggag gcaaagccat
    35041 gctaggaaca tcttccggat tccatgcaga aacgatcaat cttctagaat caggagtctt
    35101 tttgattgct tcgatcactt gtttgatttg atcgataaag ccaccgtctt ttgtttccca
    35161 gtgacgccat tgatagccgt agatgtgtcc aagatcgccg tatgtttcag caaaggaatc
    35221 atcggttagg atcttctcgc aaaaaagctt gctctgcttt ttatattctt cattgaattc
    35281 aggatctgtc aaacatctat ggccaaaatc agtcatatct ggtccattat agtctggact
    35341 tttgatgtag cgttcaaatg cccattcatc ccaaatatgg tttttatttt gtaatagata
    35401 gcggatattc gtatctcctt ttaaaaacca aagcaattcg cttttgatca atccaaaagg
    35461 tactcgtttc gttgttaaca aaggaaaccc tttttgcaga tcaaaccgca tttggtatcc
    35521 gaataagctt aatgtgcctg tgcctgttct gtcttcttta cgatgccctt cttccaacaa
    35581 ttttcttcct aagtcaagat acgcttgttc catccttttt cctcctaatc ttctacccat
    35641 tcactcaagt attcccaccg tgccattttt tcttcaagtt gtgcttctgt ttcagatacg
    35701 tcgttttgca attcttgtaa tcgagtgaaa ttatctcctt gatgattcat ctcttcttgg
    35761 agcaatgaga ttttttcttc aagttctgca atctcatctt caatcgtttc ccattctttt
    35821 tgttccatgt aagataattt cttcttttca gcaggttcct tgctctgtat ttttggtttc
    35881 gctttttcag caggctgctg ggtctctttt tgcttcagta aataactgga catgtttccg
    35941 taataaagtt cttgttttcc gtttccttgg aatactaata acttatctgc taccttgtcc
    36001 aagaaataac ggtcatggga aacagtgatc acagcacctg gaaaacttct aaaataatct
    36061 tccaaaatcg tcaatgttgc tatgtccaaa tcattggtcg gttcatccaa taataaaaca
    36121 ttaggttgct gaatcagcag tttcaacaaa aataaccggc gtttttctcc gcctgaaagt
    36181 ttgcctatca gtgtcccatg cataaatctt ggaaataaaa atcgttcaag taattctgct
    36241 actccgattt gactgccatc tgctgttttt gcttcttcag cagcttcttg taagtaagca
    36301 atcatccgtt tatctggtgc catttcttca ttttcttgtg tataatacgc aagatgaacc
    36361 gtttcaccga tagacattgt accgctatcc aggggaattc tgccagctag aatattaagc
    36421 aatgtcgatt ttcccgcacc atttttacca gtaataccta gtctttcccg agattgaatc
    36481 aataaatcta aatgttctag cagtgtttgg tcatttatac ggtaactgcc atctttgatt
    36541 tccagtactt ttttccctaa acgttgtgtc gctaggttca gctctaaatc atcttcttgg
    36601 tttacttggt acaagttctc ctttagatct tcgaaacggt tgatccgtgc ttgctgcttg
    36661 gtgccacgtg cctgtacgcc agccctcatc caagctaatt cctgtttata aagccgcttg
    36721 cgcttttctt cttgttctac ttctacacga tcacgttctg ctttttctaa gacgtaagct
    36781 tcataatttc ctttatactc gtataatttt ccaaatgaaa gttcgaagat tcggtttgct
    36841 actctatcta gaaaataacg atcgtgggta accatcaata gtgcgccacg gtattgtttc
    36901 aaatagtttt ccaaccactc gatcgcttca taatctaaat ggtttgttgg ttcgtctagt
    36961 aatagtaaat cgggttcatc aatcaacact tgcgccagac taactcgttt tttctgacca
    37021 cctgataaag tactgatttt tttatctaat tcactgattc ctaatttttg caaaataatc
    37081 tttgcgttgg tatctgtagt ccaagcatct tctttattca tttgttcttc tgctttagca
    37141 taacgttttt gcacatcagg gtcttctccg ttttccccta gttcgatcaa tgcttcctca
    37201 tatgctcgaa cagtttggat caaggggctg tttccttgaa agactgcttg gagaacagtt
    37261 aattcatcgg aaaattcagc tgcttgagaa agataaccaa tacggtaatc agttggataa
    37321 aacactgttt gccgatcacc gtcgctgcta tcgataccgg caataatatt taagagactt
    37381 gttttacccg tcccgttcgt cccaatcaac ccgatacggt ctttttcgtg gaccaaaaaa
    37441 gaaatctggt caaacaaatc tttttcacca taagtttttt tgaggtcagt tacttttaat
    37501 tctttcaaca tgttccctcc ttatcttgga tattgtatca taaaaatagt gaacttcagg
    37561 aaaatttttt tggtatttgt tctttttgtg ttctcaaaaa aagaacagga ccgattcgat
    37621 cctgttctga tatgttttgt tttttataaa acttgaaaat ttttaccttt ttattttatt
    37681 ggactcgttt tttttgactt tgctcgaaac gcaaatcgga aagaaaagtt atcagatggt
    37741 ttgttcatcg tataagattc taacaaattg atattcctgc gatagctgtt cgcagaaaac
    37801 gtcgtgattt tttcagcaac ttcgtattca tcaatgattt ttctttcaag ataataacct
    37861 cttagcattt ttttcgtaaa cagactatct tgctggatca tccacgaaga aatcaaattc
    37921 cccgagttca tttttattcc ttcaagtttg cgttgctgga taaaaaattg aaccatcgtt
    37981 tcatcataaa tatcagtcaa gttatctaaa ctttcaataa tcaactgagt atttttttga
    38041 tatgtctctc tgatttcttg gatatcttca tcactaataa atgtctgacg atcagcatat
    38101 cttctttgcc aaaacatttt cggatggaga acgattcgca ttaacctacg gacaacaatg
    38161 aaccaaaagc cgataaaaga caaaatctgg cttgtgatag aatgttcgaa tcgagaaaga
    38221 aaacgggagt aaaaacgata accattagaa gaaagcttgc ccgaacgata actttcatcc
    38281 aatccatccc gttcgatcga caaaatcagt gcttgtattt cttgtacttc ttgcttttca
    38341 tcatcggtta aatcttccaa gtacagatca cgtatccgtt cttgataatt ttctatcact
    38401 gcttcagtgg ctaaaaattc ttttgtatta cgatcaattt cctttatttc ttttcgcaaa
    38461 acttcaacga cctcttctaa aatcatcaaa gcattcaaat ccgtgctttt cgcttcctct
    38521 gtttccgtca gcataggcaa gacaatgatc cctatgacca aagtcactaa gatgacaccc
    38581 gccgtcagaa acaaaagcaa agaccgctgg taaaacatca tattattgat tgaaaatggc
    38641 aaaataaaaa tcgtagccaa actgactgtt ccctttacac cgccaaatgt taagatcaag
    38701 atctcgttca actgtttttt gaacttttta ctgccatctt tgaatacata aaaaagactg
    38761 atagaaatga atctgatcac aaaaagcatg acagagatta atacgataat cgctaataaa
    38821 agaccgtttg gatataaacc gtctccccat actggcgaga acacttgcgt caattcgatt
    38881 cctaaaaata taaatactaa tgcattcagt gtaaaagcaa tagttgtcca tgtactgtgg
    38941 gacaagctcg acaattctgc atcaaacaca gagattttcc taaaaccaga agcttggaga
    39001 atacctgctg ccactgcagc aatgattcca gatacatcaa atgcctctgc caatacatag
    39061 gctagaaaag gaagcaaaag ttcgatcagc agataccctg tgacatcttg tgctgatgct
    39121 ttttcgatca aatgaattat tttttgtttg aaccagacaa gcaaaaagcc gatcaaagct
    39181 cccccaatac tagaaactac tagactcatc cccgcgttta ctgctgaaaa agatccggtg
    39241 atcagcgcac caagagcaaa ctggaaggca gtaacaccag atgcatcatt caaaagacct
    39301 tctccttcca aaatatgcat cgccttttta gggatattca ctcgtccaga aagagaactg
    39361 actgctaccg catctgtcgg acctaacgct gctccaaaag caaagcaggc agcaagtggg
    39421 acacttggca acaggaatga taacgtcgca cctaccccga caagggtcaa aatgactcct
    39481 ccaaatgcca agaacaaaat tgtcccgaaa tttttgagaa tcgaagaaat atctgctttt
    39541 tctccttctc taaataacaa tggtgcaata atcataacta agaacaattc aggttcaaaa
    39601 tcgattgatt gtccccactc tgtcagacct aataccactc caaggaaaat ttggatcagt
    39661 ggagctggaa tcattggcaa aatgcggcta gctatgttcg ataaggtgat cgttactgca
    39721 aatacaataa ctaactcagc aaattccatc gtccatcccc tttatttttt attttgtgta
    39781 agcattttta aaaattgttc ttttttcttt aaaattttgg tttcgactac ttcaacctgt
    39841 gtttggttcc cctctaattc acactgtaca cgttttgact ccaaacgaag cagatcatgt
    39901 ttcagacgtt ccaactgacg atacatcaat atatttcttt tacgcaactt acaaagtatt
    39961 tgacggcggt catccatcga aagggcgcca tagcgatcat gcaacagttt tttttgttgt
    40021 tcaaaactct ctctcatttt ccagatctcc tatacacaaa cttccatcat tatagcaaag
    40081 ttctttttgg atgaaaaata tttcattgtt tcatcgttta ttgctagtta gaaatttcca
    40141 taaatttttc aacgcctcat tctattccaa atagtatcag atatcaagta aaacttaaga
    40201 aagataaaag tttctgacaa aaggatgttt actttcccat tcctgccttt cttgttcttt
    40261 tatcacaaca aaaaaatggg gctgcgacaa aaatcttgtc acagtccctt attctgaata
    40321 aacggtgtgg aagagacagc ctatcagtag taataagaca catctctttc acagcctcat
    40381 ttttgcgtaa aaatcgattt attcgaaaaa ttccccgtat acatcttcta cacgttgatc
    40441 tggattgatc acgatataca agttacctgt tttttcgctg atttttgttg attgatattg
    40501 attgtctaat ccttctgttc ctttatctag atatgggaag tacacaagat caggtgtgcc
    40561 atttcggtaa aggatttcca ttcgctcgat atcttcgtat ttcaatgcgc gattaaacat
    40621 gcccatttcc agtccgccta ggttgatatc atgtgtcgca actgcgtccc cttcatggaa
    40681 gatttcgatt ttaaatccag cacacggatg gatttcaaca aattcactgc cattgattcg
    40741 tccaaaactt gttgtgattt gtttgatcca aagatcgccg atatgacggc ggttgatcgt
    40801 ccaggtttct ccatttctaa aataaaattt tagcgctgtc atttctttac tcattaaaaa
    40861 gactccttta tgatgtaatt tattcttgta ctacctcagt ttagcacaaa tttatgtaag
    40921 tggtcacatc ttatgttttt tcttctaatg acgtatctgc ccactttaag tagccaacaa
    40981 agctgagcag atacagtcta gtgtttttta aatcaatttt tctttcgata aaaaatccaa
    41041 aacctgtttt ttatcatttt ttattctttg ttctacgacg gctttttcag ctaattgcaa
    41101 agcttcacta acccacttac caggtttttt tccagtttct tctagcaaga ttttaccatc
    41161 tactgctaat tcgcttaatg acttgatagg taaacgatca taggattctc ctacggcatc
    41221 catctgaggt gtttcgtcaa aatagatcat caattcttct acttgcagtg catgtttttt
    41281 gcctaagaag tacaattcga atggttccaa agctccagtt tctcgttgtc gtagtccttg
    41341 gacaagtgct tgaacgtctt taatcatttg gttagaacac ttccacgatt tcaaaaagga
    41401 acgaatctca ttttctttta gatccagttg gctgatcaat aatgcccatg cttggctctc
    41461 ttctttcaat gggacggatg gtaattcagc aaaacgtaaa agtgcttcgc cttcttcttt
    41521 caaaccggga cagcaaatgt agcactctgt ttcaataaat gcatggattc ccgcggcccg
    41581 ttcatttccc aagagcatct tgatgaattc cacatttata cgttctacag atattttacc
    41641 taatagctcg tggttttctt tgatggcgta gaaggtttct tcctctaatt tgaaccctag
    41701 ttgactgaca aaacgcaacc cgcgcatcat tcgtaaggca tcttcatgga accgctcatg
    41761 aggattgcct acagctcgca agatctgttc ttctagatct tgcagaccat cgaacaaatc
    41821 gatgatttct ccatcttctt tcaaagcaaa cgcattgatc gtaaaatctc ttcttttcaa
    41881 atcttctgcc aacgaacgaa cgaattcgac atgatctggt ctacggtagt cctgataagt
    41941 tgattccgtc cgaaatgtgg tgatctcata ttgttcatca ttttctaata ccaacacagt
    42001 tccatgttcg atcccaatat ctatcgtttt tggaaaaagc tgtttgattt cttctggaaa
    42061 tgcgcttgtt gcgatatcca catcatggat tggctggttc agcaagatat cacgaacact
    42121 tccacctaca aaataagctt catatcctgc attctctaat tttttcagaa ctggaattgc
    42181 cttttggtat tccagaggta attgttttaa tttcattttt ccaacctttc ctgcgtgttt
    42241 cttgtcctga gataaagact cagttgcatg gtgactgttt gacaagtccg tttcagagtc
    42301 ttcaaggcca ggaccacttc atcttgttta ctcgtcctta cgctcaaagc gattatggtc
    42361 tctttgattg aatttttcca tgtttttttc aaaaacttca gttaagtcga tattcaatga
    42421 attagccatg atcatcgtaa cgaataaaac atcaccaagt tcttctgcta ccgagttaac
    42481 tggttcagaa gcctttttgc ttttttctcc ataagtgtgg ttgacttctc ttgccaattc
    42541 acctacttct tcagttaatc gagccatctg agctaatgga gagaaatacc ccaccttgaa
    42601 ttgctggata tattcatcca cttcctgctg catactgtat aaagaacgct ttttatccat
    42661 ctttttccct ccaaaactat cttaccaaaa ctttaacgca aatcctagat aagagcaatt
    42721 gagaaaatcg aatttccatg ctacatttag gaaggaattt ctaaatggaa attctgtgaa
    42781 aatacataat tgaaagctgg aattcatatg gtcaaacgaa aaacagtaaa agaagttctt
    42841 tttatcatta taggaacaag tatttatgca tttggtcttg tttacttgaa catcgctaat
    42901 catttagcag aagggggagt ctcagggatc accttgatct taagagcttt atttggtatt
    42961 gatccagctt atacgacttt attaatcaac atcccattga ttttactagg aggaaagatc
    43021 ctcggtaaac gttccttggc ctatactgtt ctaggtacac tttctttgtc attcttttta
    43081 tggatctggc aaagggttcc actagaaatc aatttacagc atgatctact gatcgtttct
    43141 ctacttgcgg gtttagttgc aggtgtagga agcgggatcg tctaccgcat gggtggaaca
    43201 acaggcggca gtgatattat cgctcggatt ttagaacaaa attacggcat cactatgggg
    43261 cgttccctat tggcatttga tatcatcgtc ttacttgctt cattaaccta tatcgacttg
    43321 aaacgtatga tgtatacgtt gattgtttcg tatgttttca gtcgtgtgat tgattcgatc
    43381 ttagatggcg gatattctgc aaaaggtcta cttgtcgtct ccaacaaaag cgaagagatt
    43441 gcaccactac tgatgaccgg tctgcaaaga ggtgtgactt tcctttctgg agaaggcgca
    43501 ttctctggta attcaaaaaa aatcatctat atggtcgtta gctctagtga attaactgaa
    43561 gctaagagga tcgtccacga gattgacgaa aaagctttcc tttcaatcat caatgtgcat
    43621 gaagtggaag ggcaaggctt tacttatttg aagccacaga caagattact gaaaaaacta
    43681 taagaaaaaa gacaaagata ggctatgagg aaagattcca catccagctg acctttgtct
    43741 tttttagtac tatactattt ttaactaatt gttatgttca cttacccggt tacttttctt
    43801 ttgtgtctgc aaaaggatct ttcggttaac ttcatcagat aatttcaaaa aagcatccct
    43861 atctccatct tctagtgctt gatcgatttg agcataaagc atttttagtt ttgcctcttc
    43921 ttctttttct tgaaaatatc gttcaattaa ttcagtatct tcttcactga ctgaagaatc
    43981 ccaagaagaa aatggattgt cttctaagat agatagatat tctcttgtct gccaagcatt
    44041 ttcgaatata cattccacat ataaaggctt tttccaattc aaacggattt catgaaaaat
    44101 ctggtctgtg tccgtaaatt cttttccctg caaaaacaat ttcatgggtt ccccttcaac
    44161 agatagatca ctgatctgca gtcctcgatc cgcaaaattc gccccttcaa caaagtggac
    44221 attgcttaaa atcgcttcat gattggcaag ataattcaag atccaaaaaa cttctcttcg
    44281 gctaaaagat acatgattga ccatccacgt taaaaaattc ttcttttccc gtacatcaat
    44341 tagcattttt ctcacctctc tgttaggtca tctaataatg acagtgcctc catatctcct
    44401 ggttcaagtg ttaaataata agtcaataat tcttgcgctc ttttcagctg cccttcttct
    44461 cttaagaaaa aggcatactc tttcaagaaa tctggttcat gtgatagttc atgataagcc
    44521 tgttcatagt gtacagctgc aacggtaaag tcttctaatt cattataagc atgggctagg
    44581 ttccattctg cataaggatt agcagtttct tccatctgat tgatcacttg tatcacatct
    44641 tcatatcttt catcatccaa gtaaagatta cttaaagtaa gtaatgtttc atcttgtttg
    44701 tctcctactt ctaaagcttt cagtaaaagt tcttctgctc gctgtctatc atgcagacgg
    44761 aaagcatttt ccgaggccag gtgataaagt tcgacttggt aaggattttc tttgattcct
    44821 tcttctaaca ctgtttgtgc ttcttcaatc atttcttctt cttgaagagc ttgtcctaga
    44881 tatagatata acgattgata atgcggatta acttctcgaa gttcttccag atagttgatt
    44941 gcctgctgat tttctttcaa ttgaagattc gtaaaagcta gctggaacag tcgatcatct
    45001 gtcctttctt ctttcagtgc ttcttctaaa ggtacgatag ctgcttcgaa ttctccttgc
    45061 atacttaagg attgtcctaa acgttcctgc atcgagattc ctgaaatttc atttatcccg
    45121 cttgccaaca atgtttcata aaccactgct gcctctttga aacgatcaac tgaaaaatac
    45181 aattcagcta gcgcaaactg gatcaatggt tcatcaggaa gcaacccagc tgcttctttc
    45241 aattttgctt cactcacttc agggattccg ataacttggt acaaatcagc tatcgccaat
    45301 aagctttgcg gatagtaatc actttgctta tccacttttt ccaggtattc aaaagctaag
    45361 tcgatctcat tatcttcgat tgcaatttct gctaacggga tgttcagtcc gtcattttct
    45421 gggtattggt tgatcagttg ttcaaaaatc tgttttgctt cgatcaaaaa gccaatagcc
    45481 agcaattctt cgcctagttc agctaatata tctgctgggt cttcttttaa tgctttttct
    45541 aaaaagattt gtgcttgtga caagtcttca tctgataaag cttgcaacat tttttcgcta
    45601 tttgtattca tttcaattcc ctcttatctc actgacgatt tgttgaaata ccgtcattgt
    45661 tctcaataat tctttttcgt ttggatgttg gtatccgtct attataccta ttttttctaa
    45721 aacaagtatt tttttgcttt tttctgccaa aaaaagaact ttttccaaat actccatctt
    45781 tgaaatctgt tccagccagt ggattggata gccaagcaag tccagccaca caaaaaagtt
    45841 ttttagttgg aaatcaaaag gatatgcttg gagattccac acgaaatgaa acatcattcc
    45901 taaaaatttt ttcatactag cagaaagtag atggccattt tcggtcagat aaaaagcttg
    45961 ttcaaacagt ttaccataat ttgacaggtt ttctgcctct aattgataaa aatagatcat
    46021 ttcttcgatc atcccggcaa acggccgagt cgttaattgc tttttatttg gatagttttt
    46081 atacagattt tgcaaaaaac gatgatcaca aatcattccg caacagataa aagtcaaaaa
    46141 gtcaatctgc tttccgtcca cctgccgctc gctgattgtt tgatcataaa cgatgcgttc
    46201 tggcagattg atggtttgca gcactacacg atttggtagc tgcaaaatcg tccgctgcgg
    46261 tgttaatgct cggcttagag aacggatcga tacagggatc atccatagtg atgaatcaag
    46321 ggttgtatgt ttttgaaaaa aacctgccag atccatgatc ccttcatttc caaatccgat
    46381 gataaacatg gattgatttt cgggatagcg tttgacgaaa tcaagcaggt tctttaattc
    46441 tgttaaatga ttgcattgag tgttggcaca aatataccag tcattgtttg cttgatttgc
    46501 aaataacggg ttgatcttat ctgcaaacaa atcataatag cgctgattcg ttaaaaataa
    46561 gattggctga tcaatggaaa gttctcttag ctgactgact agcgtttctc cgtaaaacac
    46621 tttcgtcaac gcatcttcta cttgataatc aagttccata cgcatccctc ctcttcttat
    46681 ttttatttta ccattagtaa gcaaattcta gaaaagataa gagcaaagct cacctcataa
    46741 cttgcaaata aataaaaatt aaataaaata catgtgtttt ctatgtatga catacggatt
    46801 cggaatctct gtctcttatt tattcctttt ctgctaggaa ataaaaagaa aagcacttgt
    46861 agccctagag ccacaagcgc tttttcaact gtcttattta acagcgtctt ttaatgcttt
    46921 acctggtttg aatgcaggta ctttgctagc tggaatttcg atttcttcac cagtttgtgg
    46981 gttacgacct ttgcgagctg cgcgttcgcg aacttcaaag ttaccaaaac cgattaattg
    47041 aactttttcg ccgttagcta aagcatcttg aatagttgaa aatacagcat ctactgctgc
    47101 agttgcatct tttttagtta aatcagtagc tgaagcaact ttttcgatta attctgcttt
    47161 gtttgccatg gatagattca cctcctccac aagagatatg atagaaatat catttagtta
    47221 aaactttctg agtggtccag aagtttaaaa agaaatattg attaacttca atattctgtt
    47281 atcacgatat catatgaacc gcttaatagc aaggttttta ccaattttca catgcttttt
    47341 ttagtcatag aaacgtaaca aacgaaactg tctttttctt ttgaaaaaaa aacgtcgata
    47401 tggtaaaatg cctattttct cctacgaggg ataattcgga tggacgtccc ttcaaaagta
    47461 aatgcttttc tgatttgatt ttctaagaaa cgttcataag aaaaatgcat caattcctct
    47521 tcattgacaa aaacgacaaa tgtaggtggt ttgacagcga cttgtgtagc ataaaaaatc
    47581 ttcaggcgtt ttcctttatc tgttggtgtt ggattgattg ctacagcatc cataatgata
    47641 tcattcaaca cagccgaaga gatacgaaga ttctggttca tacttacttg ttcgatcatt
    47701 tcaggcagac gttccaaccg ttgtttagtc actgcagaaa caaaaacgat tggagcgtaa
    47761 tcaagatacc ggaattcatc acgaatctct tgttcgaaat cacgcattgt attggtttct
    47821 ttctttacaa gatcccattt attcacgacg ataataattc cacgaccagc ttcatgggcg
    47881 taacccgcta ctcgcttgtc ttgttcccga atcccttctt cagcattcag taccatcaaa
    47941 acgatatcag aacggtcaat ggcacgcatt gcacgcatca cgctgtattt ttcagtattt
    48001 tcgtatactt ttccgcgttt acgcatacca gctgtatcta tcattaagaa tttctgacca
    48061 ctttcagaaa cgaagtgggt gtcgattgcg tctcgggtcg ttccttcaat atccgaaaca
    48121 attacacgtt cttctcctaa aatcgcattg atcaaagagg atttaccaac atttgggcga
    48181 ccaatcaaac taaacttaat cgtgtcgtcg tcctcttctt cagtctcttc agagaaatac
    48241 ttcactgctt catctaaaat atcaccaatc cctagtccat ggcttcctga aacaggataa
    48301 ggatcgccta gccctaagga ataaaattca tagatatcgt ttctcatttc tggattatct
    48361 actttattga ctgctaaaat cactggttta ttacttctgt ataaaatttt cgcaaccatc
    48421 tcatctgcgt ctgtcacgcc ttcgcgtccg ctgactacac aaatgattac atctgcttct
    48481 tcaatcgcga tttctgcttg atgcttgatc tgatccataa aaggttcatc gccaagatca
    48541 attccgcctg tgtcgatcac gctgaattct cgacctaacc attcaccttt tgcataaata
    48601 cgatcacgag ttactccagg agtatcttcg acaatcgaga tacgctcacc agcgatgcgg
    48661 ttaaaaatcg tagacttacc gacgttcggg cggccaacga ttgcaattgt tggatttgcc
    48721 atattatttc ctcctcacaa gtttccaatc tcacttagtt taacgaatgc attgccaact
    48781 ttcaagcata gattgtcaat taaacgaagt atttttattc cttattgaaa tactaatcgc
    48841 ttttcaaaag aaaaactgag acaaaagcag ctgctttcat ctcagtcaca gatacaaaat
    48901 caattatttt tcagaatcat cggttgcttt ttcttctact tcttctttca acgcatcacc
    48961 taaaatgtct cccatagtaa atccagtatt ttcttctggt aattcataag actcaacatt
    49021 ttttggttct tcttctgaag ctggtttttc ttctaaagct ttgatactca atgcaatacg
    49081 atgttcttct ggatggatct ctaatacttt cactttcact tcgtcacctt catgaagcac
    49141 ttcatgtggt gtcgcaatat gtttatgtga aatttgagag atatggacta gcccttctac
    49201 tccagggaac acttcaacaa aggcaccgaa gcttgtcaaa cgtttaactg ttccgtctaa
    49261 tactgtgcca actggagctt ttgtatcgat ttctgaccaa ggtccaggct gtgtgtcttt
    49321 gattgacaag gaaacacgtt cttgttcagg attgatagac aacactttga catttacatc
    49381 atcattgaca gccaatacat cacttggttt tgctacatga ctatgtgaaa tctctgagac
    49441 atggactaga ccgtcaattc cgcctaggtc gacaaatgcg ccaaaatcag taagacgtgc
    49501 aacacgtcca ctgagaacat ctccctcatg gatagttgaa agaatttctt ttttcttttc
    49561 ttctctttcg ttttccacta ccgctttatg cgataagatc aaacgatttt ctgatggttc
    49621 gatttcgatg attttaaagc tcaatgtttt gcctttatag tctgaaaagt ctgcaacaaa
    49681 atgatcttcc accattgatg cagggacaaa accacgtaca ccgacgtcaa cgactaaacc
    49741 gcctttgaca acatttgtaa caggtgcttc gatgaccttg ccctcttgga aatctctttc
    49801 gatatcttcc caaacttttt tagcatctaa acggcgtttt gaaagtagat agctgccgtt
    49861 ttctttatct ttgccgatcg tactgataac gactaaatct aatacatcac cgacttttac
    49921 tacttcgctg atatcttcta ccggcaaagt tgataattct tttgccggaa cgactccttc
    49981 aactccagcc ccttcgattc cgacaatcac ttgtttgtct tcaatgacta aaacttcgcc
    50041 cttaacaaca tcgccaactt ttacttcttg tacgcttttc attgcgtctt ccattgactg
    50101 gttgttctct tgattttgat caaattctgt cataacactt gtcctcctaa ccaacattct
    50161 gccgtaagta cggtactgtt tttagtttaa ttgattattc aaaaaaaata aagcgatttc
    50221 gttcgttttc cttgaaaaaa caattgtttt tcattttttt tcttaattta aaaaagaaga
    50281 taaccttttt tcaaaacaac agcttttatt gctgcaacga cttctggaat cgatttgcca
    50341 gtcgtatcga tacgaacagc atcctctgct tgcttcagcg gagagacgtc ccttgttgaa
    50401 tcataatggt ctctttcctg tatctctttc gtcagtgttt caagatcagt cggaattcct
    50461 ttttcttggt tctctttgaa acgtctttct gcacgctcac ttacgcttgc tactaaaaag
    50521 attttcactt ctgcattcgg taatacagct gtcccgatgt ctcttccgtc catgacaacg
    50581 ccaccgcgtt cccctatttt tcgttggaca gcaactaatt cttctctgac ttttccatgt
    50641 gcagagactt cagaaacagc ttttgtgaca tctggttgac ggatttctaa agtcacatct
    50701 tttgatgcta tgaatacatg ctgtccatct tcttgttgcg aaaaagagat aggatacttt
    50761 ttgatcagcg aaactagccc ttcttcatct gaaaaagata cattgttcgt aatagctaaa
    50821 taagttactg cgcgatacat ggcaccggta tctgtataaa tataattcaa ttcttttgct
    50881 aatatcttgg caacggtact tttcccagat gaagcaggcc cgtcgattgc aatactgatt
    50941 tgtgtcatga tataactcct ttttctcaga aaaaaagccg caaagcgcgg ctattattat
    51001 ttaattctta attcttgacc agggtaaagc ataaagttgt ttggatctaa gccattcaat
    51061 tcaaacaatt gatcagtggt gatccctgca cgctcagcca cttgttttgg accttcgcct
    51121 gcttgaacag tcgttgttga accggctgca gctgatgaag aaggtgttgt ttctgctgca
    51181 ttgctgcttg aatcccctgc ttgataagtt gcctcacttg aggaagtcgg tgttgtctct
    51241 gctgcaatac tgctttctga cgttacacct gtatcttgag acacagtaga ttcttccgcg
    51301 attgtactag attctactgt tgaagtctca gttgactcct cttttgttgt acttgtagaa
    51361 ctttctacta ctgatgtcga agtttcagaa gaagttgctg ctgttgcatt gttgcttgat
    51421 ccatttctga tccagaaata agcacctgct ggaatagcga tacataagac cagtaaaatc
    51481 aaaaatagcg aaagaaataa tgtatttcct ttcttttgtt gtctctgaga acttcttgat
    51541 gaaacttcat catctgtatc gtagattggt tgttcccatg gttcttgtgt ttcgttattt
    51601 tctgtagatt gatgtctatc ttttctgctc actgttttac ctcctaaact ctatcgtgtg
    51661 tattatacca taggaaaaag tgtcttgtcc taaaaaatca atgttccatg ttttttttaa
    51721 ataaatttag taaaatcgtt ccccaatgaa gttgttcagt cttagaaaaa agcttcctct
    51781 ttggcagaag ttctgctttt gctccatcaa tatcacaaca attctgcggc ttttcggaga
    51841 gtttttcgcc aaaataagct aagatgaatt ttctgcggca gttttcttca ttaatatagc
    51901 gaagcatctg ctgcaggcgg aattcttttt ctttttcctg acgtttgagc cgttccaccc
    51961 aattttctgg tttttcagac tgctggatca gctccatcca ttttttttgc agctcatcaa
    52021 agggagcttg ctctgtggca tattccaaat aaagctcaaa actttgtcgc tgttctctag
    52081 aaagttgatt aaagaaataa tggatacgtt catccccact ttgataaaaa agaattgctg
    52141 cactttcttc ctgatctctg cctgctcgac cgatttcttg tacatagttt tctagactgt
    52201 ctggcaaatc ataatggacc acataacgga tatcagattt atcgatcccc ataccaaatg
    52261 cattcgtagc aatgagaaac tgcagctgat tttttacaaa ctgctgttga agttgtcttc
    52321 tttgagctgc atcaagacca ccgtgatagt atccaactgt aaaacgttcc ctgaataaat
    52381 gatacaatcg ttcgacttct ttttttgttg cacaataaat gattgccgct ccttgtgctc
    52441 tttccatgaa gtgttcaagt tcttgttctt tttgctgcgt tttccgaaca aaaagcgaga
    52501 tattttgccg attgactgat tgtctgatct cttttggtgg tcgatcaaat agtacatgac
    52561 gtatatctct tgctactaaa tctgttgctg tcgccgttag tgccagtgtg accgggtttc
    52621 ctaaaagctt tcggattttt cctaactgtt gatattctgg acggaaatca actccccatt
    52681 gagacacgca atgagcctca tctactacat ataacgcaat attttgtttt ttcagatgct
    52741 ctaaaagaga aggattagtc aacatttcgg gactcaaaaa caaaaattta tattgcgata
    52801 gatgacggag gacgtatctt ctttcagatt gcgaaagtaa actattgaaa gcagctactc
    52861 gtttctcccc tcgtttttgt aattgcgtca cttgatcttc cattagagaa agcagcggtg
    52921 acacgatgat gaccagacct tccattaaat accccgtcaa ttgatagcaa aggctttttc
    52981 ccgttcctgt aggcaaaata gcgagagtat cttctccatc aagcaacgcc tgaataattt
    53041 cttcttggcc gggacgaaat gaatcaaatc caaaatattt ttttaattca tttattaaca
    53101 ttctgtatcg cttcctttct caatagatag atttgataca aacgaaattc tccataatct
    53161 aattgtccat ctacattcca ttcgctaaag cgccactctc gcacggaatc cttatttggt
    53221 tccaaacgtt cgattgtttc tgcagagaga atccactcgt atggaaaatc atcaaaaaac
    53281 aaggcccact caataagatg atctgttact gttcctcttt ttaaatgacg gattgccatg
    53341 atctggtcaa ttgtctgacc attcataaac atctgcttgg tgatcatcat gctttgattg
    53401 aaattttgct gtaataatga atcgatcaaa gcgtgccaaa gcgaatctgg ccgtttttcg
    53461 atttgtgcta acaaaaggtg gatacatctg ctttgttgca gataaaccgc tgactcatca
    53521 ttgttttttg ccagctgata aggaagcagc cctgttcgct catttccaga taattggtta
    53581 gctaaaaaat ctgctgcttc ttctggtatc tccgaaaaca gctgcaccaa actctcatac
    53641 gcagtatcaa tcaaaatttc acgtggcaat tggctttcac taagccattt ttttagttgg
    53701 aaagtataaa aaggacttgt ctctgctgga agatagtctt gaatacccgt tgccagatta
    53761 gaaattactt gaacagcaaa tttaatgagc cgccaactag tttcccctgt tctgccatag
    53821 cggtcaaaac gcaaaccagt atgttctatt tgatgttcgg acaacatatc agcccctgca
    53881 ggcgttagct ttgcttcatt ttcatttatt tcgatccaac cttgctgcgc aattttttgc
    53941 aaaatttgat aaaacttatc ctgcttcaat tctggaaaac tgttatggat aaacaaaagt
    54001 tcgtgggtaa atccataaat aagtactgaa gtcgttcgtt ttcccacaag gagttggtaa
    54061 agagaactaa tccgcagctt gtgtcctgat gaaaatagcg ataaaataaa aacaatcata
    54121 ttattctcct taagttagga tggttagaaa tgtcactact atgtaaactt gtacctgaac
    54181 gctgtattgc atgcggtctt tgtcaaatcg aagcgccaga tattttcgat tatgatgaag
    54241 aaggaatcgt tttatttgcc caagaaccag aggctcatca acagttcgtg atgccggaag
    54301 aagaaacata tgtaaaaaaa gcgtatcaaa aatgtccagt tcgagcgatt ttattagaaa
    54361 ataaaatata aatataaacg aaactttaaa aacgtacact aagttctcta aacttgaaaa
    54421 taagccggga tctgtttctc ttgttccgtg aaatcccgac ttattttgaa agattcattt
    54481 gctatctata gttaattgac tagttttttc acggacccta taggacccat ttatattatt
    54541 tcaacttttt cagccagttt tccaatgtat tgacgatcac agtttgctgg tctgcgttag
    54601 aaatttctgc ctctccatct cctttttgct caccatagct gccaaaacct ccatgatttc
    54661 cgccattgat tgatacatac tctgttcttt ccggtaaata ctgcttagct tcttgataag
    54721 cttcctgatt caatacttta tcttttgtcg ccgtgatcga taaaacagga acagctgatt
    54781 ctgtcaatgt tccttttttg tcgggataac ttgccatgaa gaatacccct tttaaatgtt
    54841 tgtctgaatg attcttattg acataacgac ttgccattac gccacctaat gaatggccac
    54901 cgatcacata atcggaattt ggaaaatctt ctttgacctt atccgctttg tttccgccca
    54961 tcactgctag atcaagtggc attttgacaa tagcgactga aaagccgttt tttgctaaat
    55021 cttgcgccca aatactataa ctttctggct ctacgagagc accaggataa aaaataagta
    55081 atggagactt tccatgattc tttttctctc cttcaaaaaa taagtaatct tctttgtttt
    55141 ttccttcttt taccaacact tgcgcttctg acgatggttc gtacgtattt tgctgtatat
    55201 agtaataacc ggctccaccg agtaatccta aaatgatgac taagccaatt agtattcgtc
    55261 taaatatttt cactttctcc caccttttct atctgagtga cccctttttc ttaatcaagt
    55321 atgattatac cttaaaaaaa taagagagaa atgcaaactt tccagcatca aataaaaaaa
    55381 gacggaatat ccatttgaat atccgccctt tttgtatgtt tatttttatt tagcacataa
    55441 acgatacaca gcttctgcat aaatttccat tgctttatac atactactta attcccatcg
    55501 ttcattcgcc tggtgcataa agtcaggagt atcagggaac atcgcaccga atgccacaca
    55561 ctggttcatc gtacgagcaa aagtagcgcc gcctgataca aacggttcag tcatgtctcc
    55621 agtaatatcg cgatatgtcc ccaatagatt tttcactaac tcactgtcta gtggcacgta
    55681 taatgaatca aggaagtcaa attcttcata agtcaaccca tatttttctg ctgttttagt
    55741 taatttagct actaaatcat ctttcttgaa tgtaacaggg attctcatat ctacaccgat
    55801 ttttgtttgt tctggtgtga tttccaaact tgcaaagttc atcgttagct cgccggattg
    55861 ttcatcttct gttttgccca cgacagcttc accagttgca ttttcttgaa ctaattttcc
    55921 taagaaattg attgggccaa aatcaaatac ttctgaaaga gcaattgcca gacgagaaat
    55981 agcattcact ccttgtgctg catctttcgc gtggatactt ttaccaagaa cgacgatgcc
    56041 ttcaccttgt tcttcgaaat cgaattcgtg ttttttcaat gcttctttta cttcagaaag
    56101 cttttctcca gagtaaggag ctgcatcagg aacaacattc aacgctcctc cagcttttac
    56161 agaaaactcg ttcgttcctg gtcctgtcaa gtaagcttgc aataagcctt tttctgcata
    56221 aattaaagga aattcagcat ctggcgcaaa tccttgcgta attccttctt ctttttcgtt
    56281 atatttttct aaacaacgcc ataaattttc ttcatctgta ccaaagataa aacgaatacg
    56341 tgtcttgaat tccacgccag catcaatcaa agctttgacg gcatacattg ccgcaataga
    56401 aggacctttg tcatcttggg atccgcgacc aactaaccag ccgtctttta tagttggatc
    56461 gaatggtttt gtttcccaat tattttcgtc ccctgctgga acaacgtcca tatgacacaa
    56521 gataccgaat aattcatctc cagagccaat ctctgaataa ccgtaatagc cttctggatc
    56581 tttaaatgtt ctaaatccta gcttttcact gatttccaat actttatcca aagcttcaat
    56641 cactttttta ccgaagggat gtccttgtcc tgaatctgct tcatctagta cggaaggaat
    56701 tcggatcaat tcgctcaatg attctaatgc ttgttcatga tgttgttctg tcacaaattt
    56761 tttcatgata ttatcctcct aatatgttaa aaagggtcat gtcaaattcc tccgacacaa
    56821 cctcttcttc tcaaataatg acgttttaag cctgctccat cagcaagcag aatgttctta
    56881 acaaatgatg aaagaaaaaa cactcactga atggagctat tgacttatcc acgtgcttat
    56941 cgatagccgt ttatcggttt cttcattctt atgatagacc ttgtacctgt atttgtctag
    57001 gtttagaaca ttgctgctac acctaggatc acacagatgg ctacaaataa tatcgcaatt
    57061 aatttaccgg taaatttcca ccacactgaa acatcgaaac gagcaattgc tacagcaccc
    57121 attacgatag cagatgttgg tgtaattaag tttactaaac cagatgcaga ttgataagca
    57181 gtgattacta aatccttacc gacgccggca aattcaccca taggtcccat gattcccatt
    57241 gttgcagcag ctaagccgga agttgaagga atcaagaatg acatagggat atagaaaata
    57301 aatgtaataa tgatgaatac acttgaagat aatgaactta atccttgttc gccccaatgt
    57361 aagatggtgt ctgtgattaa tccattgttc ataaccactt ggataccacg agcaacagct
    57421 actacgattg ctacaccgat caaatcagaa gcaccagcta aaaattcaga gacaaattca
    57481 gactctttca tgccataagt gattgctact acgatggaca ttactaagaa taacatagta
    57541 atttctggga agtaccagtc acctaatgga agcatgcttt tacctagaac cgtacctagt
    57601 accggaaggt ttgttaacca gtttgtcaat gattcaaaaa tcgtgaaatt cgcattcaag
    57661 gttgtccaag gaatcaaact gatgatcatg ataccaaaag taataaagaa caatacatta
    57721 acatgttttt gttttttcgt gattacttct tgacgaccga ttgcttcaat atcgaaatgc
    57781 ttcatatctt ctttacgttg gttgtatact aatgattttg ttggatcttt ttcgatcttt
    57841 gatgcataac gatacacata tacaatagaa atagctaaca aaataacgaa catcaagaat
    57901 cgaagaccaa taccttcacc catcgaaatc ccaacagctt gtgaagctac gcctgttgca
    57961 aaaggattta ccgttgaagc tagacagcct acttgagacc ctactaatac gattgctaca
    58021 gctactactg tatcaaagcc tacagccatc atgactggga tcagtaaagc atagaatgga
    58081 atcgtttctt ctgccattcc gtaagtagag ccgcctaatg caaataacag catcaatact
    58141 ggaataagca ttttttcttt tcctttgaat ttcttcacaa ctgaagcaat cccggcatct
    58201 agtgcccctg ttttgttgat aatacctaaa aatccaccaa caaccaaaat gaagaaagag
    58261 attgggattg cagcaggtgt ttctttagtt agctcccccg ctggtacacc tagcatccca
    58321 ttgattggcg ccatgaaaat atcccacagc ccttgaggat tttgttgtac tcgttcatat
    58381 gatccagcga cgatgttgcc tgcatcgtct acactatagt gcccagctgg tataaaccaa
    58441 gtaagtacag cgattactgc aataataata aataagaccg tatacgctga aggcatttga
    58501 aattttttct tttttgtttg ttccatcttc ttcttccttt cttttccgtt agagacaaca
    58561 attttggctc taatgttatt ttatcaaaaa gagtatcaga acaaaaataa taacacgcat
    58621 tttgtaaaac ttttgttcaa aaagttcgtt tttccctatt ccctcctctt ttttcaggtt
    58681 tttaaagctt tcttcttttt cattgtagcg tttccttttt tttataaaca aacattttcg
    58741 tcttttaaaa ggagaagaat ttcacatttg caaagaattg attgattttt atgcataaat
    58801 caaaaaaata ctcaaaacaa acaaataaaa cgtttacatt attgcatgtc ttgtattttt
    58861 ttccaaaaaa aaagaccaag aaatttcttg atctttttga tatttatttg actgctgtat
    58921 gacgttgttc gaactgatgt tgttttcgac ttaaccatgg aagaagttgc gcatgcagca
    58981 ctaaaaagac agcactcacg atgaatcctt tgatcaagtt gaaaggaacg atcccaatca
    59041 gtacatagtt tgctaaagac atgcccagca tctgtgttgc gttcagccct aaaaacatta
    59101 agtaaagagg cgtaataacg aaataatttg cgatactcat aaaaatagtt agagcgatgg
    59161 ttcctgtaat gacacctaaa aatttgtttc ttcctttatg ctgtttcttt acactaaaga
    59221 aataataaat cgggaatgta aagatcgttg ttgataagaa actagcagtg tctcctacca
    59281 aattatccgg agaaaaacct gtagtcagca gatgtaatac tgaacgcaag aacgctgttg
    59341 caactccggc cgctggaccg aataagaaca tgctgatcaa taccgagata tcactaaaat
    59401 cgatcttcaa aaaactaaaa gccggcatga tcggaaaagc aaaatactgt aaaactaatg
    59461 cgatcgcagc aaacatcgct actgcaacca ttttctgtac acgggtgttc ttcatttgaa
    59521 atcctcctgt taggactcct cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa
    59581 ctctattttc cagggaaaat agagttagag ttatttgatc aagcgtcgtc aaataagctc
    59641 tatcttcttt atccagacta tactgtcggt atcggaattt caccgattca gctatttttt
    59701 tgcaaaatag gtcgtggact ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg
    59761 aaccttatat aatttttgtt tacatattca ttatacatat ctattcaaaa aacgcaacat
    59821 ctaacactta taaaaagtaa gtgttcattt tgagtggttc agcaaactgc tgacttcttt
    59881 tttgttcagt tcacggtaat ccccaggacg taggccttgt aaagtcagat ctccgtattt
    59941 ttcacgtttt agtttttgca caggaagacc aactgcttga agcatatttt tcacttgatg
    60001 attacgtcct tcgtggatcg tcaattccac gatactgtgg tttgtcttag gatccactga
    60061 taaaatttgg aatttagctg gagatgtttt tcgtccttcg atctttattc cttttgtcaa
    60121 aggtgcaagc atcgattttg tagcgatccc ttttaccttt gcaacgtaaa ctttatccac
    60181 ttcatgtttt ggatgtgtca ggcgctgagc aaaatctcca tcatttgtca gtagcaagat
    60241 ccccgaagta tcgtaatcca gtcgacctac aggatagata cgttctttta ccatcggcaa
    60301 aagatccgta acaacttttc ttcctttgtc atctgcaaca gcagaaataa ctcctttagg
    60361 tttgtacaat aaatagtaac cgtattcttc ttgataaatt gggacttcat ccacttccac
    60421 agtgtcatgt ttcccaactt ttatccctaa ttcccttaca acttgtccgt ttacttttac
    60481 acgtcctgca aggatgaatt cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat
    60541 tactttctgc aatctctcca ttacaattat tctcctttat tcattccatc aaagaataga
    60601 tctggtgaca cgtcttcttc aagtgaaatt tccatttctt gtgtatcagg cagttcttct
    60661 aatgatttca aaccaaaata atctaagaag tagccagttg ttccatataa tatcggccga
    60721 cctggtcctt ctactctacc tttttcttca atcagtttat gggcagctag acgctgtacc
    60781 gctcctgaaa cctgaactcc tcgaatctgc tcgatttcag ctcttgtgat tggctgttta
    60841 taagcgatga ttgctaaggt ttcgacggcg gcttgagata atgtgtttga tgtcccttgc
    60901 gcatatcgct taagtaaggg agccagctct tttttagtcg ttaatacata atggctttcc
    60961 gtttcaaaaa tatgcaaagc tgagtgcgag ttttgagtgt aagattcttt taattctttc
    61021 actaaacttg tgacttctgc ttcgctgaga tttaacagtc cgcttaattc ttcattactg
    61081 atcccttcct cgccaacaat aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc
    61141 ggtatctcct actgctggat aaagcataat tgattcatag ttttcttttt gttgcgcaat
    61201 gatctgccct tttttcatca attctagcaa agccataaaa gtagtgacaa cttcactttt
    61261 agaaggtgac tcaaaaaaag aatcgaaagt acagccatgc gcatttcttt ttatacgctc
    61321 ttcgataaat accattcgtt cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac
    61381 tttttgagaa aatttcttac gttctaagat tttctgcatg gcacggatca aatcgatttt
    61441 tttgacttgt cctcgcttca acggcttttc agcatccgct aacccatcga tgttcatagg
    61501 ttcttttgtg taataaagac ttcgttcctc tgctttttct gtcagtcttt cagctgcata
    61561 tttatattta cggtattcta aaagctgagt gaccaattct tctcgaggat cttcttccca
    61621 taattcttca tcgtctgtga cgaattcttg gacaggaagc aatgtcttgc ttttgattgc
    61681 catcaatgta gcagccatca ctaagtattc tcctgcaaca gctagctcga gtgttttcat
    61741 tgtatggata taattcatat attgctccgt gatcgtagcg atcggtatat catatatatc
    61801 gatttccatt tgctgaatca gatgaagcag cagatcgaga ggaccctcga atacatctaa
    61861 tttaatattg atctccgtca actcattcac tctttcttct ttttctttgc tagccatttt
    61921 ccaatgtaag gctctgtgat ttcagttgca cggatggaga catccgggta cttttcctgt
    61981 aattcatcta acatttgaaa gcccatattc tcaccgcgat aagacggatt caaagaaatg
    62041 tcatggatga tcagctgatc gttcgttgcc cagtaaatac caataatccc ttgaatgttg
    62101 gttgaacctt ctggcgtaaa gaaaaaaagt tcataatcgt cttcagattc gtacgtatca
    62161 atctccttca acaaagaagt tttacctgtc agtttcttat ggaaactcaa tagtcccata
    62221 gcaattttac ggttttcttt ccggtaatgt gttaacatct tctcaccttc ctatgactaa
    62281 gcccttggaa aatgctgctt ataaacatcc gtcattcttt tcttcgtgat atgtgtataa
    62341 atctgagtcg tagagatatc cgcgtgacct aacaattcct gtactgtccg caaatcagct
    62401 ccattttcta gcagatgtgt ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg
    62461 taaatcccag cctctcgtac taactgcttc agatttttcc agattccttg ccgagaaaga
    62521 cctttcccat gattattgac aaatacatgc atttcagatg cgtcctttac aagtaatgga
    62581 cgtgcttcgt ctaagtagcg ttccagccat tggatcgcat aatcacctaa aggaatgatc
    62641 cgctctttat ctccttttcc aactgtctgt accagtccaa gagaaagatg aagatctccc
    62701 aatttcaatc caatcaattc actgacgcgc atcccagtag catacatgac ttccaagatt
    62761 gcacgatctc ttatccctaa cgtttttgtc gtatcaggtg cttcaatcag cttttctact
    62821 tctgtcagag tcaatgtact tggtaatttc tgtgctttct tcggcgtatc gatatgttgc
    62881 atcggatcat gatcagtgat acgctcttgc cgtaaaaact gatggaatct tctgagactt
    62941 gagatcatcc gagtaacagt tgctgtcgct tttttttctt catgcaactt ctccagaaaa
    63001 cggatgacta aaaagcgatc gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa
    63061 tgcaagtact gttccaaatc acgttcatag ctttgtcgtg tattggcgga caatccccgt
    63121 tcaatattca agtagtggag ataatcgttg atttgctctt tcataataat ccccctcatg
    63181 cctctctatc atatcagata ccgcttcatt ctgatagtct ttttccataa tctgagcaaa
    63241 gaaaaagccg tctagacact cagacggact ggtttctttt ttctttttca tagcattcct
    63301 gacagattcc atggaacgtt agccgatgat cttttactaa aaaatgataa cgttcttcta
    63361 ctacttgctc cacttcaagt aaaagatcct cttctacttc ctcgatcgtg ccgcagttca
    63421 agcatagtaa atgatgatgg aaatgtttgg cgccttcttt tctcaaatca taacgggcta
    63481 atccatcatt aaaacttact ttatcgacaa ttttcaattc tgtcaagatt tcaagggttc
    63541 gataaacagt agcaagtcca atttccggac tctttttttt cactaaaaag aagatttctt
    63601 ctgcagataa atgctctttc tcattttcca aaagcacaag cagagtagct tctctttgcg
    63661 gtgtcagctt aaaccccgct tcatgtagct gctgttttgt ttttttcatc gccgaaatag
    63721 cattcatgac catttactca accccaacaa tttataataa ttacaaataa aaaaagatat
    63781 aacactatgt taattaaaat tattataagc tagttaagaa aaacatgcaa gggattctca
    63841 attagatttt ttgactagtt ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa
    63901 aagattacct aatgcgcgct taaatgctcc tttgctgata ccaaaagctt tcatgatttc
    63961 ttctggatcc gatttatctg taaagttgat ttgctgatct gcagaacgtt ctaaaatggt
    64021 taaaatcatt tgtgcatcat ctgatatagc ttcatatgct cttggcttca atgaaagatt
    64081 cagtacaccg tctggtcgga caccaatcac tcgacctttt atctgctcgc ctagacgagg
    64141 ttcattatat cgttcagaag gatggataaa cccaagatag aaatcttccg ttaaaacgta
    64201 cgtacctgtt aatttcaagc ggtatgctgt tcctatcaca tctttgtttt tcatttcttc
    64261 tgttccatat ttgcttaaag atttgaatat tttttcatct gctaaactgc cccaaatacg
    64321 gtcttttgca tcaactctaa gggaaatcat caagcgatcc ccttttttag gccatagctc
    64381 tgtcattgtc ggcaattcat caagagatac gacaaagtct ttatctggaa gaccgatatc
    64441 gacaaacgca cctaaatctt ttcgtgtagc agtaacgatt ccaaaagcgt aatgaccgat
    64501 acggctttta ggaatttgtg tagtgatccg attttcatgt ttttgatttt ggtaaccgaa
    64561 tccttcaact gcttcaccga ttttgtgttc accttctgat ttgcttaagc caaaggtttg
    64621 tccgtttttt tggacaaagt agtttttttc attttcatca ataataatac ctgtaaatat
    64681 agttgctaat aaatcgttca tttttttctc cctctatagt gaaaggtttt cttaatacat
    64741 ttctttgcta tcataacata atgttatcta cttgaatagc tgagccttgg tttggaaagg
    64801 tgaaaaaagg agtatgacaa cagttgtgtc atactcccct ttttcgaata aacggtgtta
    64861 aacggcaggc tcacagcctc tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa
    64921 ccatccagtt ttacagttga tggttacgat gtaaaatggc aaagcacttt ctccaatgcc
    64981 tttcatcaat gcagattttg ttgaagtggc atatagatta taatagcctt ccccattttc
    65041 gattctggct ttttctaatc tgtaacctcc ttcaacgata tatccacgtt ggatacatgt
    65101 agcaatcact ttgctttgga cacctggtgc ccacgtccat gctggattag atgcatcggc
    65161 aatatcactt gctctggcag atttgattgg ctgattcgct gagtttgaag aggaaggggt
    65221 cgaggatgcc gcttcttgct gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc
    65281 tgcagctgct gcttgagcgg cttgttttgc ttgttcttca gctgcttttt tcgcttcttc
    65341 tgcttttttc ttttcctctt cttctttttt ctctttatca gtgagcatct tatctacttg
    65401 tttcaattgt tcattcaact gagcagatag atctgtattt ttcacttttt ctactgcttc
    65461 ttttgccgtc ttgtattggt cgcgtgttgc tgaatccact acttttccgt ctttaaagac
    65521 tatagctgtt ttttctttag ctgtttgcaa ttcagcatat tgatttttag cttctgtttg
    65581 tgcttgttga atcaattttc caaaatctgt gttatccgtt tcgattgctt ggatctcttt
    65641 atctgctttc aacttgatgt catctgctag atgatcatcg acgatggcag gtgctatgaa
    65701 ccactcattc actttttgga tatctcggat cttagattgg atatcttggt aagttgtttc
    65761 taactctgaa tatccttttt cattcttaag actggataaa tcatttttta gttttgataa
    65821 atcttgatga atcatatctg cacgaataaa ttgatggtga tcatctgtat aaaaggcagc
    65881 aagtttctca tgaagttctt ccatttgttg ctgtcttttt gcttctgctg ctacttcggc
    65941 ttctactttt tgatgatggt cataataagc ccagcttcct ccaccgagta gaagtactgc
    66001 tgctaaaacg agataaggta ctttttttga ttttttcttt tgcgctttat ctggatctgt
    66061 tttttctaag tttgattctt gatgtgtttt ttcagtatct ggcacgattg ttattgattt
    66121 cgcttctgct tcaaacagct taggctggtt ctcttctttt tctatttttg cttcaagatc
    66181 agtaccctct ttgcttacag attgattttc agcatcggat tcactattta tttcatcggg
    66241 ctctgttgac tttttgtctg tgttttcgag tatttctttt ttctctgctt ccggcagttt
    66301 attcgttttt tccttttcta attgtttttc agtaaatact tcagtttttt ctgtgatatc
    66361 cgtttttacg tcttttattt ttactgtttc tttacctgtg gcgttttctc tatcagagtc
    66421 tttttcagtt tcttgttcct tttctggctc tactttgttt tcctgtgcaa ttttttcact
    66481 gatcattttg agcagctctt tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat
    66541 ataagcggat agtatcgaat tttcttcctc attatcgacg atttctttca gttcagctac
    66601 tcttcttttt tgttttgatt caagcgtttc ttcttgattc tttcctgatt cttccgaagt
    66661 ctcatgaaca gttgctttct tctgttcatt tttctctata ttagttactt gatcgtgagc
    66721 aggtggttct tcgtcttctg gttctgcttt ttcgaataat tccattacag attctagagg
    66781 taaatctttc aattccgacc attcgattgg atcgtttaat tctgtatcag gaaagactgc
    66841 atcttcagtt gatttatcct gcatagctgc atctgtattt gtttcttcaa ttgattcttt
    66901 tatttgctga tcttctgcag ttatttgatg accgcagtct ggacagatag tattctgctc
    66961 catttttttc gatccgcaat ttggacattt tttcatcact tagcgatcgg ctcctttact
    67021 aggtttacgc taccatttaa tctatcatat tctatacgtc acgccaatag cttattttta
    67081 ggatttactt aaaaataatg aatgaatttc cttcctatag aaaaaaaagc ttgagactgc
    67141 gtagtctcaa gctttttttc ttttaaaata aaccaaacag ttttactgat ttctcttctg
    67201 gtcaatagtc cacgcttatt ctaagaaatc ttttaattgt tttgaacgag aaggatggcg
    67261 caattttctt aatgctttag cctcgatctg acgaatacgt tcccgagtaa caccaaatac
    67321 tttccctact tcttccaatg ttctcgtccg gccatcatct aaaccaaaac gtaagcgaag
    67381 gacattctct tcccgatcag tcaatgtatc tagtacatct tccaattgtt cttttagtaa
    67441 ctcataagct gcgtgttctg ctggacttgt tgcttcctgg tcttctatga aatcacctaa
    67501 gtgagagtca tcttcttcac cgattggcgt ttcaagggaa acaggttctt gcgcgatttt
    67561 caagatttct cggacttttt ctgttggaag atccatttcc gcaccgattt cttctggcgt
    67621 aggttctctt ccaaggtctt gcagcaattg acgttggata cgtatcaatt tattaattgt
    67681 ttcaaccata tgaactggaa tacggatcgt acgtgcttgg tcagcgattg ctcgagtgat
    67741 ggcttgacga atccaccatg tagcataggt tgaaaactta aatcctttgc ggtagtcaaa
    67801 tttttcaact gccttcatca gtcccatgtt tccttcttgg atcaagtcaa ggaactgcat
    67861 gcctcgtcct acataacgtt tagcgatact tactactaaa cgaaggtttg cttctgccag
    67921 acgctgtttc gcttcttggt cgccttcttc gattttcaat gctaacgcta cttcttcttc
    67981 cgcagttaaa agggagacac ggccaatttc tttcagatac atacgaaccg ggtcattgat
    68041 ttttacaccg gttggcgcag aaagatcttc tgtttttgct tgttcagctt ttttctcagc
    68101 actcttcaag ctgtgaattg atggatcacc attttcatcc accacactga tacctgcgtc
    68161 ttcaatcttt tggatcaatt tgtccatttc atctgcattc aaagtgaatg gtgtagcaag
    68221 ctgattggat aaatcatcat atacaacttg tcgtttgggt ttgttttctt tgatgaaagc
    68281 tgctactgct gcttcatacg tttttttatc tgttccattt gccataaaag aaggcccccc
    68341 ttgagtagct atttggcttg ttttaactgt ttcgttaaat tgatgatttc aatcgtcaat
    68401 tcaagctcaa gctgtttgtt tccagttcgt ctggcttctt gctgcatttg tttttttaat
    68461 gttatttcat cagctaagcc agatttgctg atcacatcta gcaagtcttg gatttctcgt
    68521 ggcgaacttt cttctgccac cggtaaatat tcgatttcca ccaccaaccg ccttaaattt
    68581 tcatcttgca aaaagtctaa aaactctgca aggttgaatt cataatgttg tgagacatat
    68641 gcatcgaata aaaaataaac ttctgcatat gcatcatgaa taaactgaaa ttctcgttct
    68701 tttaaaatat tacggacaga aacttcttta aatgcacgat ataatagaat ctgctctgcc
    68761 ttttctactt gcgtcaaagg tctctttttc cgtgcagttt cttgtctcgt aacctgagtg
    68821 tgttgacttt cctgaggttc ttgacgattt tgctgatttg tccttcgaag atcgcgcagt
    68881 tgtttttgta tcgtctctct gccgatctga aactctgttg acagttgaga tagatacata
    68941 tcttgttcga ttggtgaaac aactcggcta agttcaacta gtaattcttc gaggtattcg
    69001 atcttgtctt tttcattatc taaatttttc ccttgtttta aataacgtga tttaaaagta
    69061 aagactgttt cgcgcccatg agccactaat tcttggaaag atgcttctcc atacttacgg
    69121 agatattcat caggatcaag cttttctgga acgactacga cagaaagtgc taatcgactg
    69181 ttttgccgaa gcaattcaat ccctcgattg gttgcttcaa aacctgcgtt gtctccgtca
    69241 tagcaaaaga ccaattcttt cgtcagcttt tccatttgtt ggatctgttg agctgttaga
    69301 cttgtcccca tagaagctaa tccagttttg ataccagcct gccatgcagc aatcacatcc
    69361 ataaaccctt cgaacaacaa aatttctccg tttttccgga tatcgcttct tgctttatca
    69421 agattaaata aaacttgtct tttattgaac agttctgttt ctggactatt caagtatttg
    69481 ggctgatctt tttcctgccc tttttcaggt gcaagccagc gtcctgaaaa accgatcgtc
    69541 tggccttgag aattacgaag tggaaacatc actctttgat aaaaacgatc agacatacgg
    69601 ccatcatcat gcgtgacaaa caatccagag cgttctaaca attcatttga aattgattca
    69661 ttcttgaata cttgctgaag aaaagaacgc tcattaggtg caaaacctat gttgaattct
    69721 tcaattaatt ctaaagacaa cccacgactt tgcaaatatt ctaaagctgg ctgtccagct
    69781 tttgtatgca atagcatgtg atgatagatt tccgctgctt tttcatgagc agcaatcagt
    69841 ttccccgtag cagattcagg atttcggaca gctatagtac gctgacgcca ttgatcttct
    69901 aacggtatct gttcgaagtc tgccactttt accactgctt ctggaaaact tagaccttct
    69961 agttcttgaa gaaaagtaaa cacgtttccg cctttaccac aaccaaagca atgaaaaatc
    70021 tgcttgtctt ctgcaacaga aaaagacggt gtacgttctt catgaaaagg acacagcccc
    70081 aagtaatttt taccagactt tttcaactgg acatattgtc caatcacctc aacgatattc
    70141 gtttgactgc gtactgtttc gataacctct tgaggaatcc gttgagccat tttttcaccc
    70201 cctgctctat tgatatcttt tgggtttaac agaagatatt tcaggagaat aaccggtaaa
    70261 aaatcgcact aactgaaatc agtgcgattt taaaggacac aattatacat gtttgattgt
    70321 atcatatttc attgcttttt caagtataac acatttctat ctcattaagc atcaatttgc
    70381 tactcttttc agtaactgat tttgaatatc agccttccag tacaatactc gaagaacaat
    70441 atttacgagc atgaatacaa agaagtatcc tacgctctct cccaacccta agtttacttc
    70501 caaaattcca tggaacagca ttgcagctac atatatccct acgatcgtcg ttactgctag
    70561 gaagatgcgc aatgggttga agggataaca agcttttaca actgccatgc aactgatccc
    70621 aatcagcaag taatacatca aagtagtagt ttcagcattt ccccatgcga atgtttttcc
    70681 aagtagatac accgcaatga tattcactac tacaagcaag gcatttggta aagcattaat
    70741 aagtgctgta ggcaaataac ggtaagtgat ttttctttta tcttgttcaa aagataaaaa
    70801 gaaggaagga tacccttcaa tcgctaaatc aatcagcgtg atttggatag ggataaacgg
    70861 aaatccaatt gctgtaacgg cacatataat cgataaaata aatgaataaa tcgttttgat
    70921 aaaaaagatg cctgagactt tcgtcacatt gttgaccact cggcgtcctt caaacaggac
    70981 ttctggcaat gtagtaaaat cagagtccaa caataccaga ttcgcaatct gacgtgttgc
    71041 tccatcacct tctgccatag caatgctgca atctgcttct cttaacgcca aaacatcatt
    71101 cacaccgtcg cctgtcattg caaccgttct gccgctttct ttcaattcat ttaccagcaa
    71161 tttcttttgc tgcggactca ctcgtccaaa aaccgtatag cgatgagcag cttctcgcac
    71221 ttccatttct tcagtaattt gagaaagatc gacatacgca tcataattcg gtaatcctgc
    71281 acgtctagcg atattggaga cagtaaaagg attgtctcca gaaatcactt tcagatccac
    71341 accttcctca tgaagataag ctagtgtttc ttgagcgttt tttcggattg gatcatcgat
    71401 ttcaaacaga gcaaccggct caacaaatgc tggtttttct tcgttcaatt gctgatttgg
    71461 agcgatcccc aacataagta cgcgatagcc attcgcttgg gctgttttta gactgtccgg
    71521 aagccgatct tcttccacca aacgttcagg cgctcctacg tacacgactc ccagttctgg
    71581 aaattccatc gcgccccatt tgcgatcaga agaaaatgcg actttgtttg tcacatcata
    71641 ccgattagaa agctcaaaat aatctctcat agcccgcata gtgatgttgt tatcactgct
    71701 ttctgccaga tacgtcccca taatttctgg gatggctttc tcgtacagcg gatgtagtgc
    71761 ttctacccgc tggatactca ttttcccttc agtaatggtc cctgttttat ccaagcataa
    71821 tgtatcgaca tgggccagcg tttcaattga atacatgtct tgaacaagga ttcgtttttt
    71881 tgccagtttg gttacccctg tcgttaatgc aatacttatt agcaatacca aaccctttgg
    71941 aagcatgcct aacaaagctg ctgcagatgc aataacagaa attttgatag aagcatcgcg
    72001 gatgaacagt gcttccaaaa atagtatgat ccccaaagga ataatcacga aactagtgaa
    72061 tttggatact ttgcgaatcg aatctacaag ttcagaacga atagctttgt gagtctttgc
    72121 ctcgctagtc aagcggacag cataattatc tgcacctact cggataactt cagccatgac
    72181 agaaccgctt gtcaaaaagc ttccagaaag caattcagct ccagtttctt tgacaatcaa
    72241 atcagattcg cctgtcaaaa gagcttcatt tgcttctgcc cgtccagata taacctgtaa
    72301 atcggctggt atctgttctc ctgctccgat ttttaccaaa tctcctaata ccagctcttt
    72361 agtagaaatc gattgttctt taccgtcccg gatcactagt acttctgatt gggaaacaat
    72421 cgataatttc cgtacaagat ttcttgcatg tatttcttga taaattccaa taacgatatt
    72481 gacgacaata attgctaaat acgccatgtt ggaatacgca cctacaaata acagacaaat
    72541 cccaatacca aagttcagaa aattgaataa cgtcaatata ttatcgctga atatttgttt
    72601 tgtacttttt gatgcatctt cctggtagtc attttgctgg ccagaagcca ttttctgaac
    72661 tacctcttgc tgtgtaagtc cattcatttt ctttttctcc attttccgcc aactttcaaa
    72721 aataatcacg tgtttaagtc tagcgtgttc caagattaaa aacaacttgt ttttataaaa
    72781 tttatagaaa aacacacgaa tgattttaca aaaaagatgc caaccttttt gtttgaatca
    72841 cccgtgtgct gtttttatta tcaatctatc tttacttata ttgttcctcc aaacggctca
    72901 tccaccaacc tagtgctgta ccggctataa aaagaacagg acaagctagt accccagcaa
    72961 ctcccaatcc cgcgtaatcg gtaggaatga tcataatcgt tgaagcaaaa actacaccta
    73021 aaatgaaatg gaatagcttg ctatatgcct ttgagaaaat gtaatccatg atttttgaca
    73081 atccaagaac acaaagaatc ccaccaattc cgatcggcac aatcacttca agtcgtactt
    73141 ctttaaatcc gtcagccatc gctttataca atcccatata caccaaaaaa ttagaagggc
    73201 tcaatcctgg gacgatcata cccaaaccaa tcaatccgcc ggcaatcagc catgttccaa
    73261 aattctgatc gacttgcccg tcaaataatt gagcgccgta taataaaaac aaataagcca
    73321 gaacaaaagt aacaaccatg atgatcacat cagaagtatc tcttcctttt ttccctgctt
    73381 ctttccataa cgcaggaaca gttccagcga tacatccgac aaaaaaccat aatatcgttg
    73441 tttcatacgc acctaataaa aaactgacta aaaaagaaag tagaaagatg cctgtcactc
    73501 cgcctagacc aacaggcaga aaaaacaaaa cattttcttt gaaatttttt gtaaggtggg
    73561 ctaaaaaaga aatcagccgc tcatacatgc caaagacagc tgctaatgca ccaccgctga
    73621 ctcctggcaa gataaatcct gaaccgataa acattccctt cacaaaccgt aacatccagt
    73681 ctttggttgt gtaccccact tcttgatttt ccattttttg ccttttttcc actatttgcg
    73741 tactccttct ttcatttaat tttattcatg attgaacatt tcctgctatt ttacttaaag
    73801 actttttcat aggtctctat tattttttca ttcattttga caatatcagg ataaaacatg
    73861 ttacctccat tatgatagag atagccgcca ttgtaaagca aggactggat ccgccaatat
    73921 cggtattgtt tcccttcctc atttcctaaa agtgggctca gcacatcttt agaatacgct
    73981 tctgctaatt ctactgtgtt ttctccgcca tgatttttta cataggcaat ataatctttc
    74041 ccaaaattat atgcttggac agctgtccat aaatcgcatc cttgtttctt tgcttctact
    74101 atcatttccg ccaaatattt gacaccttga tcaatactgt cagctggatt atcaatgatc
    74161 tcagcttctc catgcagact ttccgagctc tgcatgagat ctgtcccttt tccttttgat
    74221 tcggtcaata ttatcgcctt gatcaaattc tcgtagtttt ccatatgata attttttgca
    74281 gctgttgtta tttctatttg atagctttcc actcgtttta ccgaacgata agttcttcct
    74341 cataaaaaca acaccgcacc aataaccgtc agaatgatca tgtaaaaaag aatacggata
    74401 atagataatc gttttcgctt catttcatca cctagttatt ttgacatctt tttttatctt
    74461 actatttctt tatcttcttg gctatttctt ccttgatttc tgtgtacgcc ttaagaaaaa
    74521 atgaattttc ctgtattttt tgacatctta cgaaaatgat cgttgttttt cagtaaaaac
    74581 aattgcccaa tgactgatat aatcgttata ctttacacag taggtgatta tgtgaaaaaa
    74641 gaaaagaaat atgcaaaaat gccagatggg agcgaaattt actatgaaaa gagcggtcaa
    74701 ggcttccctc tctttttact gcatggcaat gacggcagcg gccgcttttt ttcagaacag
    74761 gtccctgtac tcgaacgcta ctataccgtg tatctagtag atagccgagg acatggtcgt
    74821 tcgacaaacg aagcatctat gttgaatttt cagttaatgg ccgaggatct gaatacgatc
    74881 atgttgctgg aaaaaatcga tcaggcagat tttcttggat ttagtgatgg agcaaatcta
    74941 gcactggtat ttgccagcag cttccctaaa aaagttcatc gattgatttt aaattctgga
    75001 aacacgcttg ttaaaggcgt tcgtttttct gccagagtca tcagtaatat tcattatgca
    75061 tgggtatggt tactctcttt atttcgccca agtcttcgca aaaatctttt agttatcaaa
    75121 cttttgctgc atgacatcgg gcttacagaa aatgatttaa aaaaaatcaa ctcccccact
    75181 ttgatcatcg tgggaaaaaa agatgtaatc aagctaaaac attctttgta tatagctaaa
    75241 actataccaa aagcttcctt tgtgcttgtg aaagaacaag gacatgaatt agctagaaaa
    75301 gatcctgaac gcttcaatcg agaagttctt caatttcttt ctgaaacata actattagga
    75361 atagaaggtg atttatttgt taaaaaatac catacaatgg ctgaaaaacc acctaggact
    75421 gttcaaaaca atctttttga tttctgtcat tgtcattatt gtcggtgaat tgatgtccat
    75481 cggtaaaact ctatctatcc agcaattagg agaaacgttt gccactatac ctgtatggaa
    75541 aagcggacta atgctattga ttggtcttat ctctgtgtta cctatggttg gttatgatat
    75601 tattttgaat cggatgcttg aacaaaagca gaatcctcgt tacctgtttg agacaagctg
    75661 gctgatcaat acgatcaaca atattgccgg tttcggcggg tttgttagtg tggggcttcg
    75721 ctctgaacta tatggacaaa agaaagagag cagaaacgtg atccaagcat tatcgaaagt
    75781 tttcttgttt ttgatggctg gactttctat ttacagtctg ctttcttttt tacttgtagt
    75841 cttcacgcca gttgctcctt ttttgaagca atattggata tggttgatcg gcggcggtct
    75901 atattttcct gtcgtctttt tatttactac tttcaaaaag aaaggattcc ttggtggtac
    75961 ctcacttaaa actcaaggac agctattgct agtttcattg ttggaatgga ccggtgtcct
    76021 gtttagtttc ctttctgttg gttatttaat ggaaatccgg attgatctgt ggcaaacaat
    76081 tccattattc attgctgctt cagtgatagg aatcgtgtct atgattccag gagaaattgg
    76141 gagtttcgat gtaatgatga ttatcgggtt gtccgctatt ggtgtgccaa gagaaatagt
    76201 cgttgtgtgg atcttgctct atcgcctgtt ctactacatc attccgtttt tgattggaat
    76261 cgtctttttc ttcaaaaata tcggttccac ttttgatcag cgttattctg gtatccctaa
    76321 acaattagca acagaaattg cgcataaaat cgtggttgtc ttattatact tttctggaat
    76381 catgctagta ctttctgcca ctatcccgca agcttttaca gagtttcgct ggcttcatag
    76441 tttgaaccca ttgaaatttc attttattat ccaatttcct agtatactat tgggatttct
    76501 tttaatcatt atgggcagag ggatcgcagc acgtgtgaaa cgagcatatt tgcctacaat
    76561 cttcttgatt gttctagctt tactttatgt tttattaagt gattttagtt tcacaccggt
    76621 ggttttcttg tctattctgc ttctgatcat tcttgcttct aaaaacgaat tatttcggga
    76681 acaattgatc tattcatggg agtggcgtac gatcgatgga atcattattg gtgcactgtc
    76741 cttgctttac atcgttattg gggtatacaa tctgccggat ttccctcatc ggcgtcacca
    76801 ttttatttcc tttttcttat ttccttctga aaagatttgg ttttcgggat tgttggccat
    76861 catcgctgtt agtttcatga tcgttctatt tgttcatttc cttcaaggag aaaagaaaca
    76921 gattggcgaa gcctttaatg aagaaaaggc cctgaagatc cttacaactt atggaggaaa
    76981 ctccgatagt caattgatct tcttaaaaga taagcggatg tttgcctatg aaaaagacgg
    77041 tgaaccaact gttcttctcc aattcgcttg cttcaataat aaatgtattg taatgggtga
    77101 tccttctggt aagaaagaag attttccaga agcaatcgaa gcttttatcg aagaaacaga
    77161 tcgactatgc tatttaccag ttttctacga aacaagcgaa gagattgtta tgattttgca
    77221 tgaattcggc tacgatttca ttaagatggg ggaagaagct tatgtagacc tgaatagttt
    77281 tacaacttca gggaaaaaaa tgaagggcac tcgtgctgtc ttgaatcgga tcgaacgaga
    77341 aggatttaca tttgatgtct tacaaccacc attttctact gagcaaatga gtactttcaa
    77401 aaatatctct gataattggt tgggttcacg aaaggaaaaa gggttttctt taggcttttt
    77461 ctctgaagat tatctacaaa gagcgcctat tgcagttgtc aaaaataccc aagaagaaat
    77521 tgtcgctttt gcaacgatca tgcctactta cacgaacaat aaagtaggaa cgattgatct
    77581 gatgcgatat gattctaaaa aagcaccttc tggcagtatg gatttcttgt tcttgcattt
    77641 gtttgcctat atgaaagaag aacatatcca atggttcaat ttagggatgg cccctctttc
    77701 aaatgtcgga acttccagaa agagtttttt acaggaaagg attgcatact tggtttacga
    77761 gtttggttct catttttatt ctttccatgg attaagagaa tataaaagca agtacgctac
    77821 aaactgggtc tcacgttata ctctatattc tagagatagt tggattgcat atgttatgat
    77881 tgctttgctg attattgata atgcgccagt tgagcagaca caaggtagaa taagtggttt
    77941 gaaaaaatgg atacgaagaa agtattaaaa actgcatacc gatagatgaa cactctcatg
    78001 atgaataaaa gagactgaag taaaacgaaa gttcatgctt ctacaatctc ttcttataca
    78061 attgaagaac aaagagcggt ccaaacagaa atcctgtttg gaccgctctt ttattttaaa
    78121 catttttcat taccaaaata ttcgagtcgc gtttcttatg cttcgatctc tgtaacgata
    78181 cctgaaccta ctgtccgtcc gccttcacga attgagaatg tcgttccgtt ttcaacagcg
    78241 attggatgga tcaagtcaac atcaatcgtc acgttgtcac caggcatgac catttctgta
    78301 tcttctggca atgtgattgt tcctgtaaca tcagttgtgc ggaagtagaa ctgtggacga
    78361 tagttgttga agaatggagt atgacgtcca ccttcttctt ttgtcaacac gtacacttct
    78421 gctttgaatt ttgtatgtgg agtaatggaa cctggtttag caagtacttg tccacgttcg
    78481 atatcgtcac gttggatccc ccgtaacaac acgcctacgt tatccccagc ttccccgtaa
    78541 tctaacgttt tacggaacat ttctacacct gttacaactg ctttttgtgt ttcaggtttg
    78601 atccctacga tctctacctc atcaccgaca cgaacagcac cacgatcaat acgacctgag
    78661 gcaacagttc ctcgacctgt aattgagaag acgtcttcca ctggcaatag taatggttta
    78721 tctgtatcac gttctggtgt tgggatatat tcatctactg tatccatcaa ttccataata
    78781 gcagcttcag catctggatc gccttgcaat gcttttaatg cggaaccttt gatcacagga
    78841 gtgtcatcgc ctgggaaacc atattcgctc aataactcgc gaacttccat ttctaccaaa
    78901 tcgatcaatt cttcatcatc gactaaatca actttgttca agaaaacaat caggtatttt
    78961 acaccaactt ggcgtgacaa taaaatgtgt tcacgtgttt gaggcatcgg accgtcagtt
    79021 gcagaaacaa ctaagattgc accatccatt tgtgctgccc ctgtaatcat gtttttcaca
    79081 tagtccgcgt gtcctggtgc atcaatatgc gcgtagtggc gtttttctgt ttcatattct
    79141 acgtgtgcgg tattgatcgt aatcccacgt tcacgttctt ctggagctgc atcaatacta
    79201 gcatagtctt gaggatttgc taggcctttt ttgcctaata ctgtagtgat cgcagcagtt
    79261 aaagtagttt tcccatggtc aacgtgtcca atcgtaccaa tgttaacgtg tggtttactt
    79321 ctgtcataat gttcttttgc cataatttac aaacctccta aataatatag atcactgaaa
    79381 aatttcagct ctcacctttt tattatagtc tttttttgac taaatcaaaa agaatacgtc
    79441 cgacttactt tttgtaagat tcgaactaag cttattatac gtcaatattg gtcaaagtca
    79501 actaagagca ctcgtctgtc ttatacgcga aaaaagtacc ttttataaat aaaaaaatgt
    79561 gttttttgag aaaaacttca agatagtcct taactttctt ttgaagcaat aagaacaaaa
    79621 tgaggaaaag tttaaaattc agctgttgcc aacaactttc aatagacgaa atcttctatt
    79681 tcgttaataa taaggaaaga ggtgaagtac atgaaaaaat taattggcgt acttgtcgtt
    79741 ttaggcatat ttctcggcgg aagttttgca gcttatcatt atttttacgg cggcgaagct
    79801 tattatacaa aaatcacgac gaatggcgaa atgtcagcta cccaagcaga taacggagag
    79861 aaattcacga cttataccta caagcaagcg gcttataata aagatggtga aaaaaaacag
    79921 gtgacgcttc gcgaagaaag agaacatcca ttaaagatga atgcgtatct taaactcaaa
    79981 gtcaatcctc gtaaaggtgt attaagctgg gaagaaataa aggcttctga agtgcctaaa
    80041 aatgcggcag aaaaaatcga taattagtaa atggtcttaa aattttatta gataaaa
//
LOCUS       DAYYBG010000003        77609 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000003 DAYYBG010000000
VERSION     DAYYBG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.003"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1597)
                     /locus_tag="ACHAUN_000176"
                     /pseudo
     CDS             complement(1..1597)
                     /locus_tag="ACHAUN_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1920..2399)
                     /gene="rlmH"
                     /locus_tag="ACHAUN_000177"
     CDS             complement(1920..2399)
                     /gene="rlmH"
                     /locus_tag="ACHAUN_000177"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8458915.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2827..4116
                     /locus_tag="ACHAUN_000178"
     CDS             2827..4116
                     /locus_tag="ACHAUN_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8458916.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4155..4760)
                     /gene="ytpR"
                     /locus_tag="ACHAUN_000179"
     CDS             complement(4155..4760)
                     /gene="ytpR"
                     /locus_tag="ACHAUN_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8458917.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4965..5429)
                     /locus_tag="ACHAUN_000180"
     CDS             complement(4965..5429)
                     /locus_tag="ACHAUN_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8458918.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5451..5771)
                     /locus_tag="ACHAUN_000181"
     CDS             complement(5451..5771)
                     /locus_tag="ACHAUN_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8458919.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5915..6994)
                     /gene="pepA"
                     /locus_tag="ACHAUN_000182"
     CDS             complement(5915..6994)
                     /gene="pepA"
                     /locus_tag="ACHAUN_000182"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8458920.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7194..7544
                     /locus_tag="ACHAUN_000183"
     CDS             7194..7544
                     /locus_tag="ACHAUN_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8458921.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7767..8714
                     /locus_tag="ACHAUN_000184"
     CDS             7767..8714
                     /locus_tag="ACHAUN_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8458922.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8771..10189)
                     /gene="pepV"
                     /locus_tag="ACHAUN_000185"
     CDS             complement(8771..10189)
                     /gene="pepV"
                     /locus_tag="ACHAUN_000185"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8458923.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10338..11183
                     /locus_tag="ACHAUN_000186"
     CDS             10338..11183
                     /locus_tag="ACHAUN_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8458924.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11187..11627
                     /locus_tag="ACHAUN_000187"
     CDS             11187..11627
                     /locus_tag="ACHAUN_000187"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8458925.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11677..11871
                     /locus_tag="ACHAUN_000188"
     CDS             11677..11871
                     /locus_tag="ACHAUN_000188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458926.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12007..12693
                     /locus_tag="ACHAUN_000189"
     CDS             12007..12693
                     /locus_tag="ACHAUN_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8458927.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13101..13790)
                     /locus_tag="ACHAUN_000190"
     CDS             complement(13101..13790)
                     /locus_tag="ACHAUN_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458928.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13765..15894)
                     /locus_tag="ACHAUN_000191"
     CDS             complement(13765..15894)
                     /locus_tag="ACHAUN_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8458929.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15942..17279)
                     /locus_tag="ACHAUN_000192"
     CDS             complement(15942..17279)
                     /locus_tag="ACHAUN_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8458930.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17531..17890)
                     /gene="rplT"
                     /locus_tag="ACHAUN_000193"
     CDS             complement(17531..17890)
                     /gene="rplT"
                     /locus_tag="ACHAUN_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8458931.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17943..18143)
                     /gene="rpmI"
                     /locus_tag="ACHAUN_000194"
     CDS             complement(17943..18143)
                     /gene="rpmI"
                     /locus_tag="ACHAUN_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8458932.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18215..18739)
                     /gene="infC"
                     /locus_tag="ACHAUN_000195"
     CDS             complement(18215..18739)
                     /gene="infC"
                     /locus_tag="ACHAUN_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8458933.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18783..18908)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19059..19691
                     /locus_tag="ACHAUN_000196"
     CDS             19059..19691
                     /locus_tag="ACHAUN_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8458934.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19693..20598)
                     /locus_tag="ACHAUN_000197"
     CDS             complement(19693..20598)
                     /locus_tag="ACHAUN_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8458935.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20683..21342)
                     /locus_tag="ACHAUN_000198"
     CDS             complement(20683..21342)
                     /locus_tag="ACHAUN_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8458936.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21658..22602
                     /gene="mvk"
                     /locus_tag="ACHAUN_000199"
     CDS             21658..22602
                     /gene="mvk"
                     /locus_tag="ACHAUN_000199"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8458937.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22595..23572
                     /gene="mvaD"
                     /locus_tag="ACHAUN_000200"
     CDS             22595..23572
                     /gene="mvaD"
                     /locus_tag="ACHAUN_000200"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8458938.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23585..24670
                     /locus_tag="ACHAUN_000201"
     CDS             23585..24670
                     /locus_tag="ACHAUN_000201"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8458939.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24667..25731
                     /gene="fni"
                     /locus_tag="ACHAUN_000202"
     CDS             24667..25731
                     /gene="fni"
                     /locus_tag="ACHAUN_000202"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8458940.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(25980..26783)
                     /locus_tag="ACHAUN_000203"
     CDS             complement(25980..26783)
                     /locus_tag="ACHAUN_000203"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8458941.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26803..27645)
                     /gene="kduI"
                     /locus_tag="ACHAUN_000204"
     CDS             complement(26803..27645)
                     /gene="kduI"
                     /locus_tag="ACHAUN_000204"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8458942.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27661..28668)
                     /locus_tag="ACHAUN_000205"
     CDS             complement(27661..28668)
                     /locus_tag="ACHAUN_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8458943.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28675..29319)
                     /locus_tag="ACHAUN_000206"
     CDS             complement(28675..29319)
                     /locus_tag="ACHAUN_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8458944.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29419..30177)
                     /locus_tag="ACHAUN_000207"
     CDS             complement(29419..30177)
                     /locus_tag="ACHAUN_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8458945.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30304..31455)
                     /gene="fucO"
                     /locus_tag="ACHAUN_000208"
     CDS             complement(30304..31455)
                     /gene="fucO"
                     /locus_tag="ACHAUN_000208"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8458946.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31537..31848)
                     /gene="rhaM"
                     /locus_tag="ACHAUN_000209"
     CDS             complement(31537..31848)
                     /gene="rhaM"
                     /locus_tag="ACHAUN_000209"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8458947.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31850..32695)
                     /gene="rhaD"
                     /locus_tag="ACHAUN_000210"
     CDS             complement(31850..32695)
                     /gene="rhaD"
                     /locus_tag="ACHAUN_000210"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8458948.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32709..33965)
                     /gene="rhaA"
                     /locus_tag="ACHAUN_000211"
     CDS             complement(32709..33965)
                     /gene="rhaA"
                     /locus_tag="ACHAUN_000211"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8458949.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33962..35422)
                     /gene="rhaB"
                     /locus_tag="ACHAUN_000212"
     CDS             complement(33962..35422)
                     /gene="rhaB"
                     /locus_tag="ACHAUN_000212"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8458950.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35575..36444
                     /locus_tag="ACHAUN_000213"
     CDS             35575..36444
                     /locus_tag="ACHAUN_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8458951.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36570..37469)
                     /locus_tag="ACHAUN_000214"
     CDS             complement(36570..37469)
                     /locus_tag="ACHAUN_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8458952.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37634..38458)
                     /locus_tag="ACHAUN_000215"
     CDS             complement(37634..38458)
                     /locus_tag="ACHAUN_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8458953.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38469..39764)
                     /locus_tag="ACHAUN_000216"
     CDS             complement(38469..39764)
                     /locus_tag="ACHAUN_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8458954.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39780..40259)
                     /locus_tag="ACHAUN_000217"
     CDS             complement(39780..40259)
                     /locus_tag="ACHAUN_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8458955.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40265..41293)
                     /locus_tag="ACHAUN_000218"
     CDS             complement(40265..41293)
                     /locus_tag="ACHAUN_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8458956.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41310..42416)
                     /locus_tag="ACHAUN_000219"
     CDS             complement(41310..42416)
                     /locus_tag="ACHAUN_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8458957.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42645..45242)
                     /gene="adhE"
                     /locus_tag="ACHAUN_000220"
                     /gene_synonym="adhC"
     CDS             complement(42645..45242)
                     /gene="adhE"
                     /locus_tag="ACHAUN_000220"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8458958.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45547..45927)
                     /gene="yajC"
                     /locus_tag="ACHAUN_000221"
     CDS             complement(45547..45927)
                     /gene="yajC"
                     /locus_tag="ACHAUN_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8458959.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46024..47169)
                     /gene="tgt"
                     /locus_tag="ACHAUN_000222"
     CDS             complement(46024..47169)
                     /gene="tgt"
                     /locus_tag="ACHAUN_000222"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8458960.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47387..48085
                     /locus_tag="ACHAUN_000223"
     CDS             47387..48085
                     /locus_tag="ACHAUN_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8458961.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48253..50892)
                     /locus_tag="ACHAUN_000224"
     CDS             complement(48253..50892)
                     /locus_tag="ACHAUN_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8458962.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51511..52542)
                     /gene="queA"
                     /locus_tag="ACHAUN_000225"
     CDS             complement(51511..52542)
                     /gene="queA"
                     /locus_tag="ACHAUN_000225"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8458963.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            52735..53909
                     /locus_tag="ACHAUN_000226"
                     /pseudo
     CDS             52735..53909
                     /locus_tag="ACHAUN_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            53913..54554
                     /locus_tag="ACHAUN_000227"
     CDS             53913..54554
                     /locus_tag="ACHAUN_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8458964.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54551..55468
                     /locus_tag="ACHAUN_000228"
     CDS             54551..55468
                     /locus_tag="ACHAUN_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8458965.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55465..56136
                     /locus_tag="ACHAUN_000229"
     CDS             55465..56136
                     /locus_tag="ACHAUN_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8458966.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56677..57165)
                     /locus_tag="ACHAUN_000230"
     CDS             complement(56677..57165)
                     /locus_tag="ACHAUN_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8458967.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57366..57632)
                     /locus_tag="ACHAUN_000231"
     CDS             complement(57366..57632)
                     /locus_tag="ACHAUN_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8458968.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57632..57895)
                     /locus_tag="ACHAUN_000232"
     CDS             complement(57632..57895)
                     /locus_tag="ACHAUN_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8458969.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58416..58556
                     /locus_tag="ACHAUN_000233"
                     /pseudo
     CDS             58416..58556
                     /locus_tag="ACHAUN_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(58698..60521)
                     /locus_tag="ACHAUN_000234"
     CDS             complement(58698..60521)
                     /locus_tag="ACHAUN_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8458970.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61119..63824)
                     /locus_tag="ACHAUN_000235"
     CDS             complement(61119..63824)
                     /locus_tag="ACHAUN_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8458971.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64130..65077)
                     /locus_tag="ACHAUN_000236"
     CDS             complement(64130..65077)
                     /locus_tag="ACHAUN_000236"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8458972.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65267..65536
                     /gene="rpsN"
                     /locus_tag="ACHAUN_000237"
     CDS             65267..65536
                     /gene="rpsN"
                     /locus_tag="ACHAUN_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8458973.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65712..66074)
                     /locus_tag="ACHAUN_000238"
     CDS             complement(65712..66074)
                     /locus_tag="ACHAUN_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8458974.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66085..67206)
                     /gene="alr"
                     /locus_tag="ACHAUN_000239"
     CDS             complement(66085..67206)
                     /gene="alr"
                     /locus_tag="ACHAUN_000239"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8458975.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67222..67572)
                     /gene="acpS"
                     /locus_tag="ACHAUN_000240"
     CDS             complement(67222..67572)
                     /gene="acpS"
                     /locus_tag="ACHAUN_000240"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8458976.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67702..69213)
                     /locus_tag="ACHAUN_000241"
     CDS             complement(67702..69213)
                     /locus_tag="ACHAUN_000241"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8458977.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69535..70887)
                     /gene="murF"
                     /locus_tag="ACHAUN_000242"
     CDS             complement(69535..70887)
                     /gene="murF"
                     /locus_tag="ACHAUN_000242"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8458978.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71040..72116)
                     /locus_tag="ACHAUN_000243"
     CDS             complement(71040..72116)
                     /locus_tag="ACHAUN_000243"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8458979.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72312..74057
                     /locus_tag="ACHAUN_000244"
     CDS             72312..74057
                     /locus_tag="ACHAUN_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8458980.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74337..74774)
                     /locus_tag="ACHAUN_000245"
     CDS             complement(74337..74774)
                     /locus_tag="ACHAUN_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8458981.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(74758..76131)
                     /locus_tag="ACHAUN_000246"
     CDS             complement(74758..76131)
                     /locus_tag="ACHAUN_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8458982.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76453..76941)
                     /locus_tag="ACHAUN_000247"
     CDS             complement(76453..76941)
                     /locus_tag="ACHAUN_000247"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8458983.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(76963..77565)
                     /locus_tag="ACHAUN_000248"
     CDS             complement(76963..77565)
                     /locus_tag="ACHAUN_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8458984.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22347 a        16543 c        14188 g        24531 t
ORIGIN      
        1 aaaactaatt aaaagaaaga ggtaactaca taaactaatt aaaaaagcta acttcatagt
       61 tggaggataa atggaagaaa cttcaatgat tgtttctcct tgtgttcctt caatcgacac
      121 tagtcctatt atcaatctct cttttttgtc catttacttt tgcgactgtt ccccaataat
      181 caaatacagg aagcgttatt ttttgtcctt tagattcatg tactgtatag actattttcg
      241 aatccatcac ttgataaggc atagtatgcc attccttgcc aattcttact tcatgattct
      301 gcaaatgttc tttgtattgt aatgcttccg tgggtgcata gtcataaagt cctccgggaa
      361 ggagtgtcgt gtaacgaata agattttctt gattcattac caagtgatct ctctgagtga
      421 caactttatg ttcatagttt gcttttgttg cagcagatac agccaacagt aataaaacaa
      481 ttgccgttat attaactact tttctttgct tagctgatgt gctcttcatg aaaacgaacg
      541 ataaaatcaa cgagccaaac agtacttgaa agcctaagat tcgccaagga aattgaatca
      601 tttgaatggg tgtattttgt aataaagacc atggaaaaaa tttggtggat aagagccaag
      661 agactaccgc actaaggtaa atcacttttt ctttgcgatt aaatccttta aaaaagaaga
      721 gtggacagat caaagaaata ataccaatga ttcctaccgt aaaggttgtc aggtcattat
      781 tcatcgcacc aatcaaacta tccgagacat ttaatgcttg gtgttgtaag gtcgttttat
      841 ctggctcatt aatcgattga accatgattt gtcggaacat cggataccaa aagtatgctg
      901 ttaaaagaat cgtcattcct actgcctttg acaactccac aatcaaccgg atgctttctt
      961 tttttcttgt ttcacttact acaaagccgc ctaagactag taagccaacg gctattacac
     1021 tcataaaagt ggaaagaaca tgtgtataaa tgagtaagct catactgact gctaatggaa
     1081 ttgcacatgg ctttttctca taaatcagtg cgtaagcata atacataact atcggtataa
     1141 ccgtaagtgc aatcgcttca gcaatagcag aacgatgata aatatcaatc gtacggtacc
     1201 cataaaatgt atataaacag ctaaatataa aagccgcatc gattcttttt aaaaatttta
     1261 gtccataatg aaacatcagc atgatagtac aaacattcaa tagccaaaca tataagatat
     1321 aactaacgat taaattatta cttatccaat aaaaaatgac tgccggaaaa aaggtcaagt
     1381 agggataaaa ataattcact ccgctcccat aatgattaaa cgtcgtaaaa ttgattggac
     1441 cagaaaaaat tgaagataac cctttgactc tagcaaaatg gaaggcaata tcccccgatg
     1501 tagaaatagt atgaacattt ctaaaataat tgattgaata aagtccagca ataattgcta
     1561 ggagaatgga caatattatt tttttcttta cttgcattac ttaattatat actcctttca
     1621 tggattgctt tatctagtat aacttaaatt ttaaataaat cgttatttat ttttactctc
     1681 acttaaaaaa gcactaaaat tataagagag ggaggtaatg gtctgaacac caagaattgt
     1741 tccaacatcg aattgcttaa ctaatgagtt caaagcttaa ttatgtggct cttatattta
     1801 ccgagcgtgg attgcgatct ctttagcgaa acaaacatct ttcataaaaa aaacagccat
     1861 atccgctttc ttggatatga ctatttacta ctactttact atctttcatt cttcttgtct
     1921 cacttatgat aaggcgcccc cgcattgatc cggaaggcac ggtagatttg ctcgacaagg
     1981 atcagacgca tcaattgatg tggatacgtc atttttccaa aagagatctg agcattgctt
     2041 cttttcatga cggcttcgct gagtcctaaa gagccgccga taacaaaaac aaggtggctt
     2101 tttccttgga tacctaattg gtcgatttgt ttagcaaaag attcacttgt aggatttttc
     2161 ccttcaattg ctaaggcata gacgtattct tgttctttga ttttcgctaa gatccgttcc
     2221 ccttcttttt cttttacttg tctcatttgc gcttcgctta aattctccgg ggctttttcg
     2281 tcaggaactt cgatcaattc gatttttgca taggcattca gccgtttgac atactcattg
     2341 ataccttgga tcaggtattt ttcttttaat tttcctactg agataatttt gatattcatg
     2401 ttttctctcc tttggtcttc atgctcactt tgtttattat aatcaatttt ttcgtctata
     2461 tgtttatttt ggctattttt gttaaaaaga tttccccaac agttatgcac aacatccaca
     2521 ggtttatcca cagaaaacag tgttatttta ccttatttca tcacattcat taggcaaaca
     2581 tacgttttat aagaatttat ccactttata cacaggctgt ggatatgttt tatcccttat
     2641 ttttccaatc ctatccgcgt agttttacac agtatctgtg gaaaaagaaa atcaaatatt
     2701 ttcatctgaa aacaatgatt tttcctaaag tttttcataa aaagttcaca gtctcaaagt
     2761 agactaaagt caacaaataa gactatacta acagtagata aatgaatgta attggaggag
     2821 gaacacatgg atagaaaaaa tgtgacacca aagatgaaaa aaagtaaaaa taatagcctc
     2881 tggcgtaagt taggtctggg gcttgttgga gggatcgttg gaggactact gacagctggc
     2941 attttttatg cagcgatggg cactggcaac aatagttcta ctgctacaag cggcaatcag
     3001 aattctgccg gtgaaacagt tgtggaaaat gtcaaagtca atgtagattc tgatatcacc
     3061 aaagcggtag ataaagttca agacgctgtc gtttctgtta tcaacttaca aagtcaaaac
     3121 caatcaagcg gttttggcgg actattcggg cagcaagaag aaagtagcag ctcagatgat
     3181 agtaatttgg aagcttatag tgaaggaagc ggcgtgatct acaaaaaaga cgggaatact
     3241 gcttatgtag tcacgaataa tcacgtagta gatggccagc aaggtcttga agtcttgatg
     3301 aaagacggta cgaaggtaaa agctgaatta gttggaacag atgcttattc agatttagcg
     3361 gtgttgaaga tcaattcaga caaagtcgaa acagttgcct cttttggtga ctcaagtgct
     3421 ctgaaggttg gtgagcctgc gattgcgatt ggttccccac taggatctga atatgcaaat
     3481 tctgtcactt caggaatcat ttcttccttg aaccgacaag tgacaagtac gaatgaatcg
     3541 aacgaaacag tcaatatcaa tgcgatccaa acagatgcag caatcaaccc tggaaattct
     3601 ggtggtcctt tagttaatat cgaaggacaa gttatcggta tcaactcaag taagatcgca
     3661 agtacttctg aatctacttc taatgtaagc gtagaaggaa tgggatttgc tattccaagt
     3721 aacgatgtag tgaatatcat caatcaactg gaaaaagacg gaaaagtgac acgtccagct
     3781 ctaggtatca caatggtcga cttatctgcc gtttctactc agcaacaaga acaaatcttg
     3841 aagattcctt catctgtaac taacggtgtc atcgtgacaa atgtccaaac agctacaccg
     3901 gctgaaaaag ctggattgaa acaatacgac gtcattacta agatcgatga caaagaagtt
     3961 tcttctggtg tagaacttca atctgctttg tataagaaaa aagtgggaga ttctgtcaaa
     4021 atcacttact atgaaggctc gaaagaaaaa acaactactg ttgaattgac gatcgatcaa
     4081 tcggcattaa aacaaaatca aaatagtgaa aattaataaa aaagaggttg tgacaaagac
     4141 ttgtcacaac ctctttattt tggaaatgct tctccgacta cggcatcctc aggcaatact
     4201 aagattcctt tttttgctgg agcatttggc aaatgcaatt cacgagcgga acagatcatt
     4261 ccatagctat ctacccctcg caaagttcct ggccagatca tcattccatc tggcatcata
     4321 gctcctggtt ttgcaacgac aactttttgt ccagcttcga tattgggtgc accgcaaacg
     4381 atttgcaata ctgtaccatt gtctacttct gtctcagtaa tcgataaatg gtcagaatca
     4441 ggatgtggga cgcatgtttt tacgtacccg acaacgatct tcggttcatg atcagcttca
     4501 agctgttctt cgaatcctgc ttctttgatc aatcgattca atttcgctaa ttcctcatct
     4561 gttaagctta cttggccgtt cccttcgatt gttagatgat cggagatatg gaaaaagttc
     4621 caagcaacaa cttgaccttg ttcatctttg atccgagcaa cattccattt gcgttcaaca
     4681 gtgttttctg ttcctttatc gtcggctata ataaccatca aggtgtctcc gacgtgtgct
     4741 ttgttataag caaaaatcat gttctttcct ccattatcct ttgctgagat ttatcttaac
     4801 aaaaaattgc attcactgca tgaaattttt ttcatgcttt atttccgaag aagcctcttc
     4861 tggaacacca tttattcgta acaagggact gtgacaagac ttttgtcaca gtccctttac
     4921 tgttttagtt aattgattaa aaaggtcgta caagttccac ttgcttaatt acttggctgt
     4981 tcttcttcgt cagaaacgat caagacatca caaggtgaat gacgtatcac gtagctggct
     5041 acacttcctg tcataaagcg ttcgactgca tttagacctg actgcccaac catgattaga
     5101 tcgatctcat atttttttgg caatttgact gccatggatt cacgaacaga accatatgca
     5161 ataattcctt ctatatttgt gtaatcaaca gatttcccat attctttaca ttcttcgatc
     5221 atttccttgg ctgtttcagt ttgctgatcg actagatttt gatcaatagg tgcatagccc
     5281 ataaagttat aaaattgctg atcgattacg tttgctacat atactttccc attattacgg
     5341 cgagccactt ctaccgcttt tttgaaagca gcaagtgctt gttcactacc gtcgatacct
     5401 actaaaatat tcttatattc ttgattcatc ttgacccctc ctaacagatg tcagcctttt
     5461 gttacacttt ctaggaacga ttcgatttcc tgttttgttt tacgatcttt attcactaga
     5521 cgacctaatt cttttccgtc ttggatcacg acaaaacttg ggataccaaa aatgttccat
     5581 tcagcggcta catctatgta cttgtcacgg tctacttcaa taaattgcca atcagaaaag
     5641 tcattttcaa tttcaggcat ttgcggtttg ataaaacggc agtctccgca ccagccggca
     5701 gtaaagaaaa atacactctt tccagtttct acgtagcctg ctaattcttc taaactattt
     5761 gggataatca ttctttgtcc tctactttct gtttatgtat ttttcaatta aattatacac
     5821 ctttctttcg aactaacaaa cattgatcct ctttttataa aaaaaatctg gcaaaaagca
     5881 atcttttgcc agattttata gtttcactat cgcattattt tccgtatacg atcgtattta
     5941 ctgttgattt atccaaacct ttcagtgttt gaatcaacat ttctcgtgca gcttcaaaat
     6001 cgcgaatact gaacatcgtt tgatgcgtat ggatataacg tcccactacg ccaatcactg
     6061 tacttgggat tccttcgttt tgtgtatgag cagcacctgc atctgtaccg ccttttgaaa
     6121 caaagtattg ataagggata ttgtttgttt cagcgatatc cagcaagtac tcgcgcaaac
     6181 gaggaagcat gatcaaacct ggatcttgga tacgcatcaa tgtaccttct cctaaatgtc
     6241 cgaatgtgcc atttttcgtc actgtatcat ctgctgcaga gcagtctact gcaaagaaca
     6301 gatctggctt gaatttattg acagaagctt tagaaccacg caagcctact tcttcctgga
     6361 cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt ttgcaacgct tctaaagctt
     6421 ctaataccat tgtacagcca tagcggttgt cccatgattt actgataata ttctttccgt
     6481 tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc tcctggaagg acaccgtaac
     6541 tcattgcctc ttctcttgat tcaaacccag catcgaacaa aatatcggtt acttctactg
     6601 atttttgtcc gcttgttcct cgcaataaat gtgggggaac ggaagaagaa atacatggat
     6661 aattcttacc agtagaggtt ttaagtgtaa aacgctgagc agaaacaacg taaggattcc
     6721 agccgcctag aggtacgact ttaaaaagcc cgttttcttg gatttgtgtc agcatgaatc
     6781 cgacttcatc catatgtgct gcgaccatta cacggggtgc atccgcttct tcatgatggc
     6841 gcagaccaaa aatgccgccc aaaccatcta actgtaactc atctaccagc ggttccatat
     6901 gactttccat ataagcgcga acatcttgtt caaaaccgct tgttccttgt aattctgtta
     6961 actctttgat tcgttgaaat gttttttctt ccatatgcta ttcgtctaaa tttcagacta
     7021 aactcctctc agacttatat tgatctgcgg ataaaatatg attttttctg cagaccgatc
     7081 gtatttatta tagcttattt tttctggtag cggttattta tttcaaagaa aatatgagag
     7141 tgtggtaaaa taagcacaag gagtaacaag tgagaagaag gaggtatgtc cacatgtatg
     7201 aagaaagcga actaagctat tttaaaggcg ggttagcagt tggaatcggt ctaggattat
     7261 tgagcggggt agcttcaacg atttggtatc atcgcaaaaa agtattgtca gcagaccaag
     7321 tacttgaaaa tatcaagtca tcttttctaa aagaaggtgc aatcgaaggt tcttggatct
     7381 cttttgaaaa ggaacctatt aggaaattcg ccgttcggtc gagtgcttat cggggaggtc
     7441 tttctcgaat agaagatggc gaaactgttt attatgaatt tttagcagat gctcatacag
     7501 gaaccgtatt agaaatttca agaaaaaaag gtgcaacaga ttaatacgtc aaaaaagtga
     7561 gagaacgaat acgcacccac tattcaaaag agaggtatca tgtccttctt ttgtagtggg
     7621 tggtttttta gatagagctt tccaaacata tattccagta ttttcttttt aaataaatga
     7681 tatttagcgg gaaaaactag catttccttt gattatgagg tatttttaat ttagcttttg
     7741 ttttctatag ttaaggagag tgaaaaatgg aacaacaaga aaaaattgct gttctgattc
     7801 cttgttacaa tgaggaagca acaatttcaa cagtcattgc agactttaaa cgagaattac
     7861 cagaagcaga tatttatgtc tatgataata attcgaccga tcgaacatac gaattggccg
     7921 ttcaaggtgg agcaatcgta aaaaaagaac ctcgtcaagg aaaaggaaat gtgatccgcc
     7981 aaatgttctt tgatatcgat gccgactatt atttaatggt agatggcgat gatacttatc
     8041 ctgcagaagc tgtacatggt ttgctcgaga aattacgttc tggtgaagca gatatggtca
     8101 ttggtgatcg cctatcaaat ggaacctact ttgatgagaa taagagacct ttccacgatt
     8161 ttggaaataa ccttgttaga aatacaatta ctagaatgta taaaacaaaa attcttgatg
     8221 tcatgaccgg ctatcgagga ttcaatcgta tctttgtcaa aagttttcct attatgagtt
     8281 ctggttttca aatcgaaacg gaattgacga tccatgcttt agataaaaaa ttcaagttcg
     8341 ttgaaatccc aatcgattat cgtgatcgtc ctgaaggcag cgaatcaaaa ttgaatacat
     8401 tctctgacgg attcaaagtg atcatgatga ttattaaaat gtttaaagat tataagcctt
     8461 tgcttttctt tgggatttgg accatcttct tctttttatt tggattggtc acggggattc
     8521 ctgtgattcg cgaatatatg ctgacaagct ttattacacg catcccttct gcgattttaa
     8581 gtactggatt gatgacttta gcacttcttt cattagtaac agggttgatt ttggatacag
     8641 tcgtcacgaa cgctaagaaa gaatatgaat tgaatctata tcatgtttat catgaatacc
     8701 acagtagaaa gtaaaaaaag gatcaagact gcaaaaataa tttgcaattc ttgatcctct
     8761 ttttaattaa ttatttcgcc aaacgataga tggcatctgc atagattgct gttgctctga
     8821 acaagtcatc aagactcata aattcgtttg cttgatgcat tgtatcagta tatcctggga
     8881 acatcgctcc ataagcaacg ccacgtttca acaaacgtcc gtaagttccg ccaccgatga
     8941 tttgttcgta gcctttttct ccagtgtggt cttcatagac ttgcaataat gtagccacca
     9001 atggatcatc cattggtacg taatgtggtt ccattacgcg agcacctcga gtaactgttg
     9061 cgccttcttg ccccactgtt tgttcgatgt cactttgcaa tccgtctact gttacacctt
     9121 ttggaaaacg gaaattcata ttgatgaagt tcgcttcctc tgtaccattt tcttcaaaag
     9181 caaatagtcc agcattcatt gtcaatttgc ccattttttc atcttcataa gcgactccta
     9241 gtttttctcc ataaaaatct tcatggacat aagcagctgc tacatggata aattgtttag
     9301 cagatcctag gaattcatag ttatctaaga acaatgccaa gaaagaacct gcattgatac
     9361 cagattgtgg gcttgcacca tgtgcgcctt taccaactaa ttcgattttt acatacgtat
     9421 tgtccgcttc aatcgttccg cttactggat ttgcttcgat gaattggtaa aatgcttctt
     9481 ccatcgcgat agctgcatct gcacttggta cttgaagtgc tgcagttgct gttcctggca
     9541 ccatattttc gcgaagtccg gaaacaaaag atttcaagac ataatctcct gcattgtcgc
     9601 ctttgaagcg taaagctaaa gaaacatttc ctttttcacc attgatgatt gggaattcag
     9661 catctggtga gaaaccaaaa tctggttctt cttcatgttc gaaataataa gccatatcgc
     9721 cccagccgct ttcttcatcg ctgccgacta cgaagcgaac tttttttgat agtttcaagt
     9781 caagttcttt aatgatcttc atcgcatagt aagctgccat acttggacct ttgtcatcgc
     9841 ttgatccgcg tgcaaagatc tttccatcct tgattactgg ttcataagga tctgtgtccc
     9901 agccatctcc tgcaggaacg acatccatgt gtccaaatat ccctagcgtt tcgtctcctt
     9961 cgccaagatc aatatgccct gcatagttat cgacattttt tactttaaat ccatcacgtt
    10021 cgccataagc taacatatgt ttcaatgcgt cgcgaggtcc aggtccaaat ggtgcatctg
    10081 gtgttacttt tgagtcatcg cgctcgctgt tgacacgtaa aagatttttc aagtcttcaa
    10141 gcaagtcttc tttacgtgct tctacttctt tttgccaatc aattgtcata aagttaatcc
    10201 tccacttatt taagtcttcg tgttcatgat accacaaatt acagaaaaaa atagttcaaa
    10261 aaatctgaca attccttttc ttatctgttt ttctctaaaa aaaatatacg ctataatgag
    10321 taaaaggagg tttttagatg ctgataaacg aacaactagt gaaaagagtc atcacacctc
    10381 gtccaaaaat ttcacataaa ggaacatttg gacgtatcgt tttgattggc ggcaatgaac
    10441 aatatggcgg agcaatcatt atgagtgctt tagctgccgt acacagcgga gcgggtctca
    10501 cgactgtagt aactgatgaa aaaaatcgga gtgctcttca cagtcatctg ccagaagcaa
    10561 tgattgtagt ttggacggat actgaccagt ttctttccgt aataaagcaa gcagatgtac
    10621 tcttgattgg tcccggattg ggtacttctt ctaagagttt ggaacgtttc aaacaagtac
    10681 tagaaaacca atcaaaagaa caatggctgg tgattgatgg ctctgcttta agtttacttg
    10741 ctgctaatca tcttttgcta ccctatcccg aacaaactgt acttacacct catcaaatgg
    10801 agtggcaacg gttgagttct ctaccgatac aagaacaacg ggataaagaa aacaaaaaga
    10861 agcaacagga aatcggtgct gtgctcgttt tgaaaagtca tgagacaaaa atttatggtc
    10921 accagcactt ctacaccaat cctttaggtt ctgctgctat ggcaacgggc ggcatgggtg
    10981 acactttagc tgggatgatc acaggatttt tagcccagtt tcctcaaaat gaccaaacga
    11041 ttgcagcagc tatttatctt catagtttca ttggagacca gctggcaaaa gaacaatata
    11101 ttgttttgcc gacacaaatc agcgaagcac ttccgaaatg gatgaattat ttttgtaaaa
    11161 aatcaatgga aaacgaggca tgagtcatgg agaaatttac aggttatgtg cgagaacctt
    11221 tttattatga aacagatcaa atggggatta ttcatcacag caattatatc cgctggtttg
    11281 aagaggctcg ggtagcctta ttggtacatc tagggtttcc ctataaaaaa atcgaagaat
    11341 ctgggatcat catccccgtt ctggaagtaa attgcagcta taaagaaatg atccattacg
    11401 gagaaaaagt gagcatccat ccaactatcc aaaagtatac ggggacacgg ttggatttta
    11461 gctacgaaat tgtgggaact gaagacaaaa aattaaaaac tactggcagt agtaaacact
    11521 gttttttact ttctgattcg caacgattag tgaagctgag caaagtcaat ccggagttag
    11581 atcagttatt ccaagcgtac gcttctttgg atgatgtaaa ttcttaataa agaccgtcta
    11641 agcatttttc ttgtattgtg tcgagtagtt cgtataatga aaagaaaaaa gcaggtgatc
    11701 cccatgtatg tcgttaaagt aatgcacggt tacatcgata aaactggctg tcgcactcgg
    11761 gaaaaaaact tagacaatct gctcattttc aaagacaaaa aagaatccga agcttttgct
    11821 aaaagaatcg gcggtcgtgt caaaccgatc caagaggtac gaccagattg attcaatatt
    11881 tttcttttcg aggctctaat aggcatgcct atcagggctt tttttgcaga ctgtgttttt
    11941 atgcccacac gatttgtaag cgatttatgt tataatagat tatatctgca tagaaaggaa
    12001 ggttaaatgg ttatcaataa tttgcaacag caagcttatg aatctatacg taaacaaatc
    12061 atttattccg agttagcacc tggaagtaaa atctccgata aagtgttaga agaacaacta
    12121 gctatcgggc gtacaccgat tcaagaagca ctgatccaac tgcgcaatca agagcttgta
    12181 aaaaccattc cacagtccgg cacttatgtt tcaaaaatcg atatccgatc tgcttcatta
    12241 gcccgttata cgcgggaaaa gttggaaaaa ccgatcctgc aagaatgcag tgcgaaaatg
    12301 accaagcaag atcaagctaa actagaaaaa atactagaac aaaccgatca ggcagtaatc
    12361 gctcaaaata aaaaagcttt ttttgatttg gatactgatt ttcaccgaac ttgttatgaa
    12421 attgctggaa aacgagaaat ttgggattgg ctagaaagtt atagtacgca cttgaaccgt
    12481 ttcagatggt tacgtttgac gatttcagaa ttagactggg gtcgagtatt agatgaacat
    12541 cagacaatgc ttcaatcaat gattgatcat aactttgatg aagtcggatt tctttgcacg
    12601 atgcatttac atatgattat cgaggagcaa gaatacgtga ttcataatta ccccgattat
    12661 tttgaagata ttcaggacag acccatcaaa taagaggttt tggaatcagc gttttgacct
    12721 gagtattaga gaaagaaacg gcaaaatagt tcctcatatt ttgccgtttt ttttgagcta
    12781 ataccgaggg tcagctgatt gcacaccgtt tattgagagg ttgtgaaaaa gctgccctgc
    12841 atcaagtaat aagaacagcc aagtaaaaat agcttcttat gtttttaact tggctggact
    12901 tattaccgca gatgcaagct tttgaacacc gttttccaag aggtcgtgag cctgccgttc
    12961 atctccgagt cacaaggaac aatttcaaaa aacagcttct catgtttgtt gaatattttg
    13021 acttgtgacc gaggagttga caggagaaca tcgtttattc ggaaaaaaag gactgtgaca
    13081 cgacttttgt cacagtctct ttatttgtaa tctaaatcat actccgtaca tcttttttct
    13141 atttttttta tttcttctaa gatcccttgg ctacgaattt cattatatgg ccaaagtaag
    13201 actgcctcta atacataagg cgaaaaatcg ctttgattca tctcttttga aaaactcatc
    13261 gctgctatct cgctcaatgc acgaatgttt gattgatgtg tataaaaagg caaccgccac
    13321 agcacaattt tttcgctttg cgtataaatt cccttaacag aagcttcgat gtggtgaaat
    13381 agttcatgtc caataatcaa agcttgcgct gtttctttag agatcgaagc tagtcctgaa
    13441 cattttaatt tttctaacgg ctccgtcatc aatcgaatcc cttcatcttc tgcataagtt
    13501 gcaaaagtga ttcgttcgtt tatttgctct gattcacagg aaacgattgg aacagccatt
    13561 tgttcacaaa tttctcctac atcggcaaaa gggtactttt ttttgatgct acatgccgtt
    13621 tcgataccac aatcgattga ttgttgtata agaattttct gcttggacaa aggaatcttt
    13681 ttccaaaata aatctcgccg aaactggtag aatccccaaa tttcttcggg gatctgttgt
    13741 aattcgttta gtaactcttc taacttatgt ttgtttgata tttccggcaa cgacgatcac
    13801 ttcttcctcc ggttccatag tagatacttc cagatccatc aaaagctgta gttgttccag
    13861 atccgtcgct gtaaaatcag agactcttgc tcctaagcag agataaaatt ctggtcgggc
    13921 aatcaattca gtttgataaa ctgccatatc ttcccaaagt tgttcgacag ctgtcatata
    13981 attggctgca gttgttttca tacatgaagc atgtccacgt tggaccttga taaatccttt
    14041 ttgatcgatg atccgaagac ttccagcatt ttttgaacga ttctgttcgc cgcatacata
    14101 aaagaaaggt gtattttcca ataagcaaac ttcattttta tttaatcgca tatcctctgc
    14161 agcaagctct agtgcttctt ctgttgtgat ctccttggtt agatcggttg ctttcacttc
    14221 cgtagaacca gtagcgatag ccgttacttt tgaagtctgt ggatcgattt ctacgtggac
    14281 ttcgatcgat tctggtgtag cgcctgattc aatcgcttta ttcatcgctt catttttcaa
    14341 cgatcgtata tcttctttac ttggcgaagg gatgatacgt tctacaacat ctcggaccat
    14401 tgcaagtgct acaccaatag aagaaatcac ttcggcgttt tcaggaatcg aatatttcac
    14461 acccatttta ttactaaagt aagtaatcaa tgaggcagcg ccgcccccta cacctacaag
    14521 agaaatctga tctttctcca agtgatactt gtctgccaat tctaaaatca ccggttcaat
    14581 cttagcatag gatttttcca tgatctgttc tgcgatgtct tcaaccgtcg tatgacaata
    14641 atcagctaat gcttggatgg cttttcgtgc agatgggaca tttccataag aaaaatgttc
    14701 ttcttgcact aatccaagga cattggctgc acatgtattt gtaatagtca cttcttctcc
    14761 gtcttccatc actaccttga cgtaatcagc agggtctcct tctttaggag agaaaaattc
    14821 aaccttagga cctttgattt tttctgtctc cgtaaatact gcataatcta gaccggcaat
    14881 atgtgcagaa cgtggaccga catcgatgat tcctgactga ttagcccgta ccatcgaacc
    14941 gcctgccaca cctagaaccc gaacatctaa tgaagagata tatgttgggt ggccacctac
    15001 aattgaataa tcgattgctg gacgtccatt tttaatgacc ccgatattgg ttgttgttcc
    15061 gcctacttca aaataaacac cattagacgc tctcaagtac atcaaagagc ccatgactga
    15121 tgctgctgga ccggaaagca tggttaaaac aggtcgtttt ttcatttcat tgatttccat
    15181 tactccgccg tctccgcgca taatcatcaa agaaacattg acccctgctt ctcgtacgga
    15241 atcttctgta gaggttgccg tatctagcat ttttggtaaa atagatgcat tgattgctgc
    15301 tgtccgagtt cttcttgtca atccatataa ttttgtgatg tcagaagcca tcgttgtagg
    15361 gatcgatttt accgatgctg cttcataaac aactcgttct ggttccccat tatctactcc
    15421 aaatgccata gaagaaacca agacctccgc tctttctctt tctaaagaag aaatcgtttc
    15481 tgaaacacgg tctacattta agtgtttgac aggtaagaat gcattgacaa tctctatttt
    15541 tttcttattc cctaaatcga tatcgttcaa tctagtttgt cgttttgcta aaaaaccttc
    15601 taatccacct tttgccatcc cgattacgcc gacttttgct acgtctcctt caatcagtgc
    15661 attagtcgct tgtgtggtag aatgtgcaac aaacacaaca tcttctggac tgatattatt
    15721 ttccagcaaa caattttgaa aagactgaac gacacctgcg gctactccac ggacatcatc
    15781 gtgggttgtt ttcactgaag attttccgat gatttcgtgc gtcgcattat cgattgcgac
    15841 tgctttcgta tgtgtaccgc caacatcaat tcccattcgc acaaaacgtt tcattttctt
    15901 ttcctccatt catctaatcg tttttatcga cgttttgttt actaggcgtt cccgtacaat
    15961 aagaacgtga tcaggcataa gatcgctccg atgatccaac cagttgggat ggatttcttc
    16021 atatattctc gagaagttac ttttgtataa cctaatcccc aagcaaccca agattgtgta
    16081 atatctaaat gctgaggagc aatcgttgtg attgcaaata acgggaacaa aaaggctggc
    16141 gacatcgttg gattaacagc taatacgaca gctaaaatag ctgagcctga accgactaga
    16201 ttcattggac cgcggaaaaa tcctagtggt gtcaatatag caaatacgat tgccaagact
    16261 aatggtgatt gcggtatcca atctccaaaa atagctgaaa aataaggaga agcatacgca
    16321 gctgaattat tgaacatcgc taaggtcatc aagaaaccaa ccattggcgc aacatctact
    16381 gcgccatctg taaataattt cgcaaactga cgacagattt ctttataacc tccgcgcaat
    16441 tttccagtaa acagcaatgc ccagatacct gctagaataa aacctaagat gattggaaga
    16501 tctaaaagaa cgacccctaa aactggtaat aatacggcta accatgagat cattggtgcg
    16561 tcgccacctt cagctggtac gtttgctgcc attgcatagc gtttcttttt gttcattgat
    16621 atattagcga ccgttagtac cacaactaag cttacaatca tcccaatcat accaatttgg
    16681 aaatagtcat tataagtata gctttcagcc gctggattga aaccagcata aatcgtttga
    16741 tattgtttga aattgacgat attagcaaag attccagcca taatcgagcc cataaatgag
    16801 aacagtgcaa ccggtactga aatacctaat gacatcaaga ttggcaacac aataactgca
    16861 atagaaatca ctggtccgat tcctgtcatc gacatgaata taacagcggt tacaatacat
    16921 agtaagctca tcgtgattct tggcttgtcc cctccaagtt caactacctt tcgaatcagg
    16981 gtagctgcga ttccgctatc tactagaaca cgcccgaaaa aggcaccaaa gaagacattt
    17041 accaagatcg atttggcgta ctcagctggc cccgtcgtat agatatgcgt caacacatca
    17101 attactcctt gcccttccat cgccgggttt ggtgaaaacg tgtttccgac tagcgctaat
    17161 cccatccata cagtagccat gatggcaaag ccgaccatca agttgtagcc ttttacacag
    17221 taccagacca tgaaaaagaa tgagataact aacaaaatac caataataat atcactcatg
    17281 ttattcctcc gtccattttg tttttctgat aagcaaacta atatatcagt caaactcatt
    17341 ctaacttatg tagttagcgc tttcattaaa gggtgaccat aaatgttcag ttttcaatta
    17401 aaaaacctta attatgctat tttagactta tacgttattg tcctgtttca tttgatggag
    17461 gattgtaaat aaaaaaagag gaaagaacat aacttttcgt tatcttcttt cctctttata
    17521 attgtttagc ttatttagcc aaagcttctt ttgcttggtc agccaatgca gtaaatgctg
    17581 ctgcatcgtt aacagccaag tcagctaaca ttttacggtt gatgtcgatt tcagcaactt
    17641 tcaaaccgtg cattaatttt gagtagctca agccattcat acgagctgct gcgttgatac
    17701 gagcaatcca caatttacgg aagtcgcgtt tcttttgacg acgatcgcga tatgcgtagt
    17761 tgtatgaatt cattacttgt tcttttgctg ttttaaataa agtgtgtttt gaaccgtagt
    17821 aaccttttgc taattttagc actcttttac gacgtttacg agttactgtt ccacctttaa
    17881 cacgtgccat gtataattcc tcctaataaa aataaatctt cagattctgt tatttgtact
    17941 tcttatttca tttttgctag ttgttggcga atacgtttga agtcgccact agaaaccatg
    18001 cttgctttac gcaattgacg gcgttgtttt tttgttttgc cgtggaaacg gtgacttgta
    18061 aacgcgcgga agcgtttcaa tccgccttta ccagtacgtt tgaaacgttt tgctgatccg
    18121 cggtgtgttt tttgttttgg catgactata tttcctcctc aaaatctttc gttatccgac
    18181 tatcggctgt ccaacagcca gttagtcatt caaattactt actgtcgttt ttcggtgcca
    18241 gcgtcaagaa catgctgcgt ccgtccattt tcgctttttg ttccactgtt gcaatatccg
    18301 cagtttcttc agctaagcga ttcaagactt tttgaccaat ttctttatgg gtaatggcac
    18361 ggcctttgaa acggatagaa gctttcactt tatcgccctt ttccaagaac ttacgtgcat
    18421 tacgaagttt tgtattgaag tcattcaagt cgatcgtagg acttaagcga acttctttta
    18481 cattgatcac tttttgtttt ttacgcgctt cgcggtcttt cttttgttgt tcgaaacggt
    18541 atttaccgta gtccatgatt cgcgcaactg gtggtttcgc accaggggca actaacacaa
    18601 gatccaagtt tgcttgttct gcaatattca atgcttctgc ttttgtctta actcctaatt
    18661 gctcaccatc tgaaccgatc aatcgtaact ctcgtgcacg aatgccgtcg ttaaccatca
    18721 tatcctttgc tatggtcatt cacctccaaa tattatgaga gaaagaaaag cttaccgaat
    18781 aaacggcaat aaaaaaacga gttctcttac agaacccgcg cgtacttcac ctaatagaac
    18841 cagtgaaaac tatctagccc agtgacatta tcaactaagg cgagaagcgg gtgcttctgc
    18901 ttttatttct caaccatcat agtatagcaa aaactaatct ttttgtcaat gtttttcata
    18961 cagtttgtct tccttttctg aaaatgatta gattagcaga ctttcttgca gtgattcccg
    19021 cttctttgct atactaaaga aaaacgaggt gaacccacat gaaaaaaatc gaacctattc
    19081 caccttctct tcctcttctt acaaagggag agttttattt agaagatgag gagatcttct
    19141 ctggtcttct gatcaacggg ggtgaccaaa gctatctcga tgccaaaaat atggtattaa
    19201 gagagagtca tatccaaaaa cttacgatgc aaaaaacagt tttacgtcat tttgaatgca
    19261 gtaatgtcat ttttgaaaaa tgtgattttt ccaatctgga gtggattggt ggcagtttcc
    19321 accaagtcgt gtttcatcaa tgtaaattaa caggtacgaa ttttgccgaa agttatcttc
    19381 gagactgtac tttcactgat tgtattgctt cgatggcttc ctttagtagt acaaacttga
    19441 aagctgtctc cttcgatcat tgtcaattag aagacagcga attttatgag gtcacttgga
    19501 aaaacctttt tttgtctgac aatcagttga ccggttcgaa ctggtttcga acttctctga
    19561 agggattaga ctttacgact aatttcttca cagcaatcgc tttatctcaa gattctttgg
    19621 cgggactaat cgtcaaccaa gagcaagcac ttgtgatcgc ccaagccctt ggtttgcaga
    19681 tcaaagacta gatcaacgaa tccgccgcca cagtgcaata agcatccaaa tagataagac
    19741 caaactgatg atcaaggcga ttccccatcc gaaaacggaa tgggtaaagg gtaactggag
    19801 attcattccg aagaatcctg tcacaatagt tggaacggtc aagatcagcg accagaccgt
    19861 caaaaatttc atcgtgtcat tcaagttgtt attcagcaaa ttattataag tgccagagag
    19921 ctggtctaaa atctgcgaag ctaagttcgt catttcaact gcttgtcgtg cttcgatcag
    19981 cgcatcgtcc aactcttctt tttccttttc tgacaacttg cgataaatag ccaatgcctt
    20041 gatttgttct aaaagtacta cattttgttt tgttcctgta actaagtaaa ccaaaccgat
    20101 ctctaaatct gaaagttgca gcagattctt gttcgttgtt ttttctctta atttcagatt
    20161 aagccgctga cgttcactat tcacttcctc gattaaagga aaaaaggaat cagaaatcaa
    20221 aaacaatgta cgaaacaata aactgtatac actcatgtct ggcattcgtt ccaaaagcga
    20281 tcgaattagt cgaatcacgt actcggtatt cggcgtggta aatgtaaaca aaccgtcttc
    20341 ttttaaaata aaagcgatag gactagtttc ataatgatta tcgacttttt gctggtgcgg
    20401 aacatttgat acaagcagta acgcttcatc aaaagaatcg tactctacgc gcgcccgttc
    20461 atttttatct agagaataag tcagcatctc gtcactaatg ccatattctt cttttaagtt
    20521 tttaacagct agcacatcgt tttgctgtac attgatccaa aaattacggt cttgattaaa
    20581 atagtgtttt tcccgcatac tctatcctcc tgacatttgt ttccagtata aagaaaaaag
    20641 tcgctcaaat aaaggtttac cctttatttg agcaactgta aatcaaatgg ctactttggt
    20701 ttggcgaatc tctgctaatt tttcttcgat cattttcaag acttcttctt ttgcttgagg
    20761 atcttccaca aaattatagc ggtctccatc gatttggatt tttggacttt ctttgtattc
    20821 ttcataccat tgatcgtaac gtctgttcaa ttctttgtaa taatcgtata gacttggatc
    20881 aaaggacaat tgttcatacg aacgaccacg tttttcgata cgctccaaca tcgtatcaaa
    20941 agatacacga atatgaacta ataaatccgg atgtttttta tgggcagcat agggaagttc
    21001 ttccatcatg ttttccagca gttcatcata cacacgaact tctgtttctg ttgctcttcc
    21061 tagatctgca tttagatgga aaagcagtga atcttcataa atcgaacggt ccaatacatt
    21121 attgtcatct ttcattgctt gtttgatgct tgcaaaacgt ttattcaaaa aataaatttg
    21181 cagcaaaaaa gcatactgtt cagggtttgc ataaaataag ggcaagactt cattatcatc
    21241 aatcgattca taaaatgcct gactatcaaa gtgttcagcc atcatttctg ttaaacttga
    21301 ttttccggca ccaattgtac ctgctaaaac gatcacgctc atttacctcc agctcacttc
    21361 atcactaaat attgtgttta actccaacct ttttaaatat acccccaata tctagtagtg
    21421 tcaattgtat ttttttctag tcaaagaaga aaaaaagtca aaaaattctt aagaaaaaag
    21481 tgttttttct ttagaaaacg tattcttagt tttacttctg agatggtttt agtaaaataa
    21541 aggaggaatg ttttttgaga cccttagtgg acaaaaaaat tcgtgaagca gcgcaactcg
    21601 ttttgggcgc tttatttttt gcttaaaaat cacttttacg tgagaaggag ttagattatg
    21661 gcaaactatg gccaaggaga atcaagcgga aagatcatat tgatgggcga gcacgcggtt
    21721 gtttatggag aaccagcgat tgcctttcct ttctatgcaa caaaagtcac cgcattcctt
    21781 gaagagctgg atgcaatgga cgatcaactg gtttcttcct actattcagg aaatttagcc
    21841 gaagctcctc atgcattaaa aaatatcaaa aaattattca ttcacttaaa aaaacagcat
    21901 gacatccaaa aaaacttgca actgaccatt gaaagcacga ttcctgctga acgtggaatg
    21961 ggatcaagcg ctgcagtcgc cacagcagtc actcgtgctt tttatgatta cttagcattt
    22021 cctttgtctc gtgaaatact attagaaaat gtccagcttt cggaaaaaat cgcccatggt
    22081 aatcctagtg gaatcgatgc agccgctact agcagcttgc agccgattta ttttacaaaa
    22141 gggcatcctt tcgactactt ttctttgaac atcgatgctt ttttgattgt cgctgataca
    22201 ggaatcaaag gacaaacaag agaagccgtc aaagatgtcg ctcacctctt tgaaacccag
    22261 cctcatgaaa ctggacaaat gatccaaaaa ttaggatact tgacgaagca agcaaaacaa
    22321 gcaatcattg aaaattcacc agaaacgttg gcacagacaa tggatgaatc acaatcactt
    22381 ctggaaaagc tgacaatcag caatgatttt cttaatctat tgatccaaac agcaaaagat
    22441 accggggcat tgggtgccaa attaactggc ggtggacgcg gtggctgtat gattgcatta
    22501 gcacaaacaa aaacaaaagc ccaagaaatc agtcaagcac ttgaagatgc gggtgctgct
    22561 gaaacttgga tacaaggatt aggagtacat acctatgttt aaaggcaaag cacgcgcata
    22621 taccaacatt gctctaatca aatattgggg taagaaaaat gaagaactca tccttccaat
    22681 gaacaacagc ctttcgttaa cattggatgc attttataca gagacagaag tcatcttttc
    22741 tgatagctat atggtagatg aattttatct agatggcacc ttgcaagacg aaaaagcaac
    22801 aaaaaaagtc agtcagtttc ttgacctttt tcgtaaagaa gccggcctct cactaaaagc
    22861 ttcagtaatc agtcagaatt tcgttcctac cgcagctgga ttagcctcct ctgccagcgg
    22921 gctagctgct ttagcaggcg cttgcaatac tgctcttaag cttggattag atgatctctc
    22981 tctttcaaga tttgctcgac gcgggtctgg ttcagcttgc cgaagtattt tcggtggttt
    23041 cgtcgaatgg gaaaaaggcc atgacgactt aagttcttac gctaagccag tcccttccga
    23101 ttctttcgaa gacgatttag caatggtttt cgttttgatc aacgaccaga aaaaagaagt
    23161 gtccagcaga aatgggatgc gtcggacagt cgaaacatcc agtttttatc aaggctggtt
    23221 agattccgtt gaaggggatc tatatcaatt gaaacaagca atcaaaacaa aagatttcca
    23281 acttctcgga gaaacgatgg aaagaaacgg actaaaaatg catggaacga ctttagctgc
    23341 ccagcctcca ttcacttact ggtctcctga cagcttaaaa gcgatggatg cggtgcgaca
    23401 attgagaaag caaggcattc catgctactt tacgatggat gctggcccta atgtcaaagt
    23461 tttagtggaa aacagccatt tatctgaggt acaagaaaca tttaccaaat tatttagcaa
    23521 agaacaagta attactgcac atgctggtcc aggaattgcg attattgaat aaagggagat
    23581 ttttatgatt gaagtatctg caccaggcaa actctatatt gccggagaat atgcagttgt
    23641 tgaaacaggc catccagcag ttatcgctgc agtcgatcaa ttcgtgacag tcactgtaga
    23701 atctgcacga aaagtcggaa gtatccaatc tgcacaatat agtgggatgc ctgtacgttg
    23761 gacaagacgc aacggagaat tagttttaga tattcgggaa aatccttttc attatatcct
    23821 tgctgctatt cgcttgactg aaaagtatgc acaagaaaaa aacattcttt tatcctttta
    23881 tgatctgaaa gtaacaagtg agttagacag ttcaaatggc cggaaatatg gattgggttc
    23941 tagcggtgcc gtgaccgttg caacggtcaa agccttgaat gttttttacg cgttgaattt
    24001 atctcagttg gagattttca agattgcagc actagccaat ttagcagttc aagataatgg
    24061 ttcctgcggc gacatcgctg ctagctgtta tggtggctgg atcgctttct caaccttcga
    24121 ccatccttgg ctccaagaac aagaaactca gcattctatc agtgagttac ttgccctgga
    24181 ttggccaggt ctatccattg agccattgat tgctcctgaa gatttacgtt tattgattgg
    24241 ttggacgggt agccctgcct ctacttctga tttggtcgat caagttcacc gttcgagaga
    24301 agataaaatg gtggcttatc agcttttctt aaaaaatagt acagaatgtg tcaacgaaat
    24361 gatcaaaggg tttaaagaaa ataatgtaac gttgatccaa cagatgattc gaaaaaaccg
    24421 acaattactg catgatttat ctgcaatcac tggggtcgtc atcgaaacgc ctgctttgaa
    24481 caaattgtgt aatttagctg aacagtatga aggagccgca aaatcttctg gtgcaggtgg
    24541 gggcgattgc ggaatcgtaa ttgttgacca gaaatctggc attcttcctt taatgagtgc
    24601 atgggaaaaa gcagaaatca ctccactgcc gttacatgtc tatagcgatc aaagaaagga
    24661 aaaccgatga atcgaaaaga cgaacatgtt tcactagcta aagcctttca tgataaacaa
    24721 aaaaacgaat ttgacttcgt acggatcatc cataaccctt tgccgcaaat agcagtttct
    24781 gatgtcgatc taagtacaca ggcagttggg tttacactaa gcagcccatt ttatatcaat
    24841 gcgatgactg gcggaagcga aaaaacaaaa aaaatcaacc aagatcttgc aatcgtcgca
    24901 agagaagcag atttgatgat tgccactggt tcagtcagcg ctgccttgaa agtcccttct
    24961 ttagccgata cttatacgat tatgcgacaa gaatatcctc acgggaaaat cattgccaac
    25021 atcggtgcag ggacttctgt cgaaagagcc aaagaagcta tccgattgtt tcacgcagat
    25081 gccttacaga ttcatttgaa tgcacctcaa gagttggtta tgcctgaggg agaccgtgat
    25141 tttaccaatt ggaaagtact tattcaagaa actcaaacag ccattgacgt ccccctcatc
    25201 gtaaaagaag tcggttttgg catgacaaga gaaacgctca atgatctagc tgctttagga
    25261 gttcatacag ttgatatcag cggtcgaagc ggaacaagct tcacccagat cgaaaacgcc
    25321 cgtcgctcca agcgggaact gagttattta gctgactggg ggcaatcgac agtctcttca
    25381 ttgcttgaag caaatgaagc agacacttct atggaaatcc tagcctccgg aggtatccgc
    25441 aatgcctatg acatatttaa agccctttgt ctcggcgcaa atgccgtggg tacttcagga
    25501 accgtactga cgcatctgat gaatcacggc gtagaagaaa ctattatact gatgaagcaa
    25561 tggcaagaag agcttcgtct gctttatact atggtcggcg caacgaatac cgcggcccta
    25621 caccaacaat ctctgatctt ttccggacca gtgaaggatt ggtgcgaagc aagagggatc
    25681 gacttagtga agtatggaag acgcacagaa aagagcgtgg gacaaaagta aagaacactt
    25741 ttgtctcacg ctctaaacac gtacaaacgg cggaagtaaa agcaactcct tcggaaataa
    25801 gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat tccttcttat ttcttggagt
    25861 taaatgcttc tgtcccagcc tcttttttcg aataaatgat gtttaatcag ctgtccttcg
    25921 gtattcgttc aatccgctga tttcacaacc tctccactaa acagtgtact aaagctcttt
    25981 taaggttgtt ttccgatata ggcaagtatg cctccgtcca catacaagac atggccgtta
    26041 acgaaatcag atgcgctaga agccaagaag acagatggtc cagccaaatc gattggctcg
    26101 ccccagcgtg ctgctggcgt acgtccaacg ataaattgat cgaatggatg acgttcaccg
    26161 ttttcttgtg tttcgcgtaa tggagcagtt tgcggtgtag caatataacc tggtccaatt
    26221 ccattacatt ggatattgta ttgaccgtat tcagaagcga tgtttttcgt cagcattttt
    26281 aatccgcctt ttgccgcagc ataagcactg accgtttcgc gtcctaattc actcatcatg
    26341 gagcagatat tgatgatttt accgccgcct ttttcgatca tatccggaat aactgctttt
    26401 gaaacgatga atggggcatt taaatccacg tcgattactt ggcggaagtc tgccgcatcc
    26461 atttcgatca tcggtgtccg tttgatgatc cctgcattgt tgactagaat atcaactgaa
    26521 cctacatctt ctttgatctg ttgaaccatc gcttgaaccg cttcttcatc ggtcacatcg
    26581 cagacataac cttttgcatc gatgcctgct tctttataat gcgctatacc ttcatcaact
    26641 gattcttgat tcaagttatt aaatacgatt tttgctcctg ctgtagctaa agagcgtgca
    26701 atctcaaatc caataccata gacagcaccg gtgactaatg ctacttttcc atctaaacga
    26761 aacatatcca tactaaattc catcattttc tctcccttat gcttatttta attgatccat
    26821 ttgcaccata tccatgtctg tataggtgat attttctccg cacattgccc agatgaatga
    26881 gtaattgctt gtacctacac ctgaatggat cgaccagctt ggcgaaataa tggcttgttc
    26941 attgtctaca actaaatgtt ttgtttcatc tggtttgccc atcatgtgga acacacgtgt
    27001 atcttcattg gcataatcaa agtagacata agcttccata cgacgttcat gtgtatgaca
    27061 aggcatcgta ttccaagcgc ttcctggttc taaaatcgta taacccattt gtaattgaca
    27121 gctttcgcat acatttggat ggatgtattg gtagatttta cgttcattca gtgtcaggcc
    27181 ttcaccagtc tccattggtt ttacttggtc gatactgatt tttacatttg gatatttatg
    27241 atgagctgga acagaactaa tatagaattt agctgggttt tcagcatctt gagaagaaaa
    27301 tacaacatgt cgtgtttctt ttccgatata gtatccgtct tgttttttca tttcttcttt
    27361 ttgcccatcg atctcgatga atcctggtcc accgatattg atcacaccta gttcacgacg
    27421 ctctaagaag taatcaacac ctagttcttt gtctaatgtg atttctaatt ctttttcagt
    27481 aggtgtcact ccaccaaaga tcattcgatc gttgtgcgta taagtcaaac tgattttccc
    27541 tggttcaaat accttttcta ctaaaaattc ttttctcaat tgttccgttg aataatgacg
    27601 gatatcttct ggactgtgtg tataacgtgt ttccatttct tgcattttct tctactcctt
    27661 ctatcgaatg atttttccag agccgcatgc taaaaattgt gcgacttctt cttgactaaa
    27721 ttggttgcag tccccatgga tcgtatgctt caaggcagaa gctgccgtag caaaatcaat
    27781 gatttcttgc ggagacatat tggataaaat cccatggagc acgccacctg caaaagcatc
    27841 tccgccacct actcgatcca cgattggggt gattctatgg acttgggatt ccacatattc
    27901 atggttcatc cacaatgtcc ccttcaattc attttcactt gctgaatgaa cggtacgctt
    27961 ggtcgaataa aacacctgaa tattcgggaa cttttcctgc attttttgat aataataaat
    28021 tagaggttgt tcttctgctt ctggcgcttt ctcgatacct aacaaataaa gagcatccat
    28081 ttttccagct gagcagatat ctactaaagg taaaagctga tggattactt cgctggcttc
    28141 ttgctgtgtc cataattttc cacgatagtt gatatcgaag ctgatcaggc agccagcttt
    28201 ttttgcagct tctatcacct gtttcgtcat tttttgccat tttttagaca aagctggggt
    28261 gattcctgaa acatgaaata tatctattcc ttggaataac tcctctagcg accattccaa
    28321 atgatccatt tcagcaaaac ttgatccggc acgatcatag atcactgagg cggcgcgttc
    28381 gccactacca gtttccatat aataagtacc tagtcttgat ccaccagata atacctgttt
    28441 cgtatctact ccataacatt gcaagtgctt tttgactgct tttcccaatg cgttatcagg
    28501 tacttttgta gcaaaaatcg cttcatgtcc gtaattagct aaagaaattg ctacgtttgc
    28561 ttcccctccg ccataatggg cagcgaatcg ttcacttgaa tcgatacggg ttccctgctc
    28621 tgtggacaat cgcaacatga tttctccgag tgtcactact tttcccataa gcagctacct
    28681 cttgatttct ttcatttttg ctgcatactg ttgcgcgact tccgttactt tgtcaaaatc
    28741 tcctgtagct gcgggtgcta aaaggttccc acctactccg actgttacga caccagcatc
    28801 gaaccactct ttcatattgt ccagactcac accgccagtt ggcatgatat tgacttgagg
    28861 catcggcgcc ttgaaggcag aaatgatact tggtccataa gcgcttccag gaaatagttt
    28921 gacgatatct accccgcttt tcagcgctgt tttgatctcg gtaatagtca tacatcctgg
    28981 taagtaagga atttgataca gattgcacat ctctgccgtt tcttgatcaa aggaagggct
    29041 aacaatatac tctgcgccag ctaagatagc caagcgagct gtgacagcgt ctaatactgt
    29101 gcctgcgcca atcactattt ctggttggtc tttgtagaat gacaaaagtt ctttgattac
    29161 ttgatctgct tgagggactg taaaagtcaa ctcaatacca gttagaccac ctttgatgat
    29221 tgcgtggcaa gcattcaaag cttcttcttt gctttttccg cgtaccaccg caatcactcc
    29281 ggcgtttttc aatcgagata aaatatcaat ttttttcatc ttctccacct atttcctatt
    29341 tagaaatttt attttataac ttggaaatct atacccatat tatacggcag aaaagaaaaa
    29401 ttgcaaacgc ttttttcatt atttttgaat tctttgctcg atttcgcttt ttgtttgctt
    29461 gagggcactg attatttgtt cttttgtctc atctgttaaa cgatatttcg gcatacttac
    29521 actaaatgca ccaatgattt gttcgttttt cacgattgag gctcctatac aaaaaatatc
    29581 acgttcgatt tcttcatcgt caaatgcgat gtgggtttct tttaccgttt tgatttcctt
    29641 tttcaaccgt aatgcattgg tgatcgtata ttctgtgtaa gggatcaacg gatgcttttc
    29701 aagataatca gtgtactgct cttcactgaa ttctgctaat acggctttgc ccatcgcaga
    29761 attgtataat ggtcgggtga ttccgatttt tgaagacatg cggatcgttt gatgttttgg
    29821 atctagtttg ttcacgtaca agatctcatt gttgtccaat acccctaagt ggatagtctc
    29881 atcgatcttt tgctgcagct gttctaaaaa cggtaaagtc aactctacca aatcgatttg
    29941 ttcaatgttt ttattcgcgt agcgaataag ctttgcacct aaacgatagt ttttctctga
    30001 atttttattt acgtagccga tcatcaacaa agtatctagt attttcaaga ctgtagaaga
    30061 ggtcattccc acaccctttg cgatcgtctg caaagagaca tcttggtttt ccgctaaata
    30121 gtctagtatt ttagaggctt tgatcaatac agttccataa ggctttttat tttccatata
    30181 atattctcct atttctttat aagaaaaatt atagcggaaa taccatcaat ttaaaagaga
    30241 tatccagata taaactacca tttctcttta caggcaattt aatgttgaag atccgcttcg
    30301 tttttacatc aaactttgat ataaagcaat gatttcttct actgtcgttt ctcttggatt
    30361 tcctggtgtg catacatctc gcaaagcatc ttctgcaatt gctggaatat ctgcttcttt
    30421 tacacctaat tcagaaatgg tttctgggat tccaaccgct ttgctcaacc gatcgatctc
    30481 accacaagcc gcttctctta catcttctaa agacatttca gctgctcctt taatccctag
    30541 aacaagagcg atctcacggt acttttctcc agtatattct ttgttatatt tcatgacagt
    30601 tggcaaaagt gctgcacacg ctacgccatg aggaatgttg taccatgcac ttaacgggtg
    30661 agccatcccg tgtaccagtc ctaaaccgac atttgagaag cccattcccg caatatactg
    30721 tcctaatgcc attttttctc gcccattttg atcgccatct acagaagctt ccaaagaatg
    30781 gccgatgatt tcgattgctt ttaaatgcag catatcagtc atttcccaag cgccttttgt
    30841 gataaaccct tcaatagcat gcgtcatagc atccatacct gttgatgcag ccaatttttt
    30901 cggcattccc atcatcatat cgctgtcgat gaacgcgacg attgggatat catgaggatc
    30961 aacacaaaca aattttcggt gatttacttc atctgtgatg acgtagttga tcgttacttc
    31021 tgctgctgtt ccagaagtcg ttggtatagc taatataggt aaacatggat ttttcgtatc
    31081 agcaacacct tctaaactaa tgacgtctga aaactctgga ttcgtcaaaa taatcccaat
    31141 ggcttttgcc gtatcgattg gcgaacctcc accgattgcg atcaaacagt ctgcctctgc
    31201 ctctttaaca aaagccactc catcttttac attttggatc gttggattag gtacgatccc
    31261 gtcgtataaa gcataagcga ttgctgcttt atctagaagt tctgttactt ttgttgctat
    31321 accgtgctcg atcaatcctt tgtcagtaat gacagctgct ttgccgaatc ctcgctgctg
    31381 aaattcaggg acgatcgact caatcgctcc ttttccaaaa tacgatgttt catttaaaat
    31441 cattcgatta ctcattttct attcctccta atttgtatta tttttcacaa taaaaacgaa
    31501 agaaccccaa gcaaccggtt gcccagttgc ttgttgctac agttcaaaga ctggcatcaa
    31561 ttcccaactg actggagagg catctggatt tgtctccatg atatcttcca tgtacttcca
    31621 ccatttttga ttgatagatg tatttgccat tttttcccat aacttttcat cttcgatcac
    31681 taagtaacca aataacaaac ttgtactcgg atcaagatag atcgaatagt cgatgacccc
    31741 gtgttcacga agtgctgtac gcatttctgg ccaaagttca tcatgtcttt ttttgtattc
    31801 ttcttgtttt tctgggtaaa ctttcatgca aaaagctttt ttcaccatgt cagacacctg
    31861 ctttcgtatg aacttcctcc aaataacctg cttttggagt gacaccaaac gcatctgcta
    31921 aatgccacag gttttcgtcc gtgattgttt gcaagattgg tccttgcgct ttgacgtacg
    31981 tatagacttc tgctgccttt tctgctgtct cgatcaaacc aaagacttca tccatgtctt
    32041 ttcctgaccc gtagattcca tgttgtggcc ataaaaccaa tcgagtttct ttcatttttt
    32101 cagctgttgc ttccccaatc tcatttgttc ccggaaccat ccacggaatg atggaaattc
    32161 cttctggaaa aacaactaag cattccgtac acatttgcca tagcgtacga ctaaaggaac
    32221 gttcgtctaa ttcatgcgta aaactcatcg ctaatagatg actagcatgg caatgcataa
    32281 cgatccgatt atctgggtct acttctaaac gggcgatatg actcatgaaa tgacttggca
    32341 actcgcttgt aggaaggcat tcgttttcca gcccccacaa aagttccagt gttttgccat
    32401 cttcagcaac tcgaacgatt ccaagattct cggcaggttc gctcgctaca tttttaaaat
    32461 atcttcctga accagttaca ataaaatagc atcctgctaa acggctggca tcaaaaatca
    32521 tcgagatgcg gcggatcact ttttcaggat ccaagaacgg tgtgatctct tcttctttta
    32581 gtaaataaga aatattcccg ccatttcttt catcccatcc caagcgataa agattcgacg
    32641 tcgtttcgat catttctttc acataaggcg cttgtaaaat atctattttt ttcaatatag
    32701 ccacactcct attttcttaa ttccaacacg tctttttgat attcctggac ttcttccagc
    32761 caatccaaac caactggaac ttgatttttg taacagaaat aattccatac atcggcatac
    32821 gggaaatctt tcaattcttc cgtataagcc attcttgaag taaagtcaaa agccagctcc
    32881 atttctttca atcgctcaac tggttccagc atagctttca gcagtgcttt ttgtgtattt
    32941 ctcgtaccga tgacccaagc agcgatacgg ttgatcgttg catcaaagaa atccagaccg
    33001 atatttgttt tttccaacaa ttcgttacgg actaattctt tagcgatttc ctgcaattca
    33061 tcatccatca aaaccacatg gtctgaatcc caacgcactg gacgggaaac atgcaacatg
    33121 attcctttgc tgaataaagc taatgaagat aatttattgg aaatcacttc tgttggatga
    33181 aaatgtccag catctagaca aacaagtttg tcacgagtca acccgtaacc catataaaat
    33241 tcatgggaac cgactgtata agcttcagcg cctaaaccaa atagtttgct ttctactgca
    33301 tcttgtacat accgttcatc taactcttct gtaaaaattt catcgagtga ttccattaaa
    33361 cgttcccgtg gggtaaaacg atcaatcgga ttatctttca tcccatcagg tacccaaaag
    33421 ttattgatac ttgtttgccc agtttctttc ccaaagtact ccgcgatttt gcggcttctt
    33481 ttcccgtgtt cgatccagta ttcacgaact tctttatctg gatgagccaa tgtaaagccg
    33541 tctttcatca ttggatgcga aaagaaagtc ggattgaagt caagaccgat ttcttctttt
    33601 tttgcccact ctacccattt ttcaaaatgt ttcggctcaa tttcatttag atcgacagat
    33661 tcttctgtat ccaaatagat agcatgaagg ttcaatttgt gtttgcccgg tatcaacgaa
    33721 taagccttct ccagatcttg tctaagctct tcaggtgttc gtgcggctcc tggaaaattc
    33781 cctgtcgcca tgattccacc ggtcaattcg ccatccggtg tcaagaaacc tttgacatca
    33841 tctccttgcc agcaatgcat agaaattttg atgtcctgta tcttttctag tacagcatct
    33901 gtatctacac cgatagttgc atatttttct tttgcttgtt cgtaatttct ttcaatcgtg
    33961 ttcatacgat tactcctttg attttattca cgtaccattc catcgtgttt tgcttatttt
    34021 catctgggta aaaagtttca catggaaacg actgtttgat tacatttctt gcttgttcta
    34081 tattcgaaaa ctgacctgta ctgagcattt gcatggcaac attcccgatt gctgtggctt
    34141 cactaggtcc tgcttctaca atgattccgg cataatctgc tgtcaattga ttcagtagac
    34201 gattattcga tccgccccct acgatgtgca gcttttccaa tttcgttcca gttaagtgtt
    34261 ctactttgat cagctcttgt gcgtaacaaa gagcaagatt atcatacaca caacgagcga
    34321 tagctcccga tgagacaggg actggctgct ctgtttcaaa acagtattgc tgaatctctt
    34381 ctatcatatt tgggggattc aaaaatcgag ggtcattgat ttccatcact tgctgcagcg
    34441 gtttttcttt tgcagcaagc tctgccaatt ccgcataact gtactcgtac tcttgcattc
    34501 gggcaacttc ctggatcaac cacatcccca tgatgttttt caaaaaacgc accgtgttat
    34561 gggcacccca ttcattcgta taattttcag aaaaagcatt gcgagaaaca ttggcgatct
    34621 ttgtttcaat ccctaaaagc gaccatgtcc cactgctgat ataaccccaa cgttctcctt
    34681 gacctggcgt tcctaataca gctgacgccg tatcatgact tgctactgtg ataaaggtca
    34741 catctggcaa atcatatttt gaaaattgtt ctttttgcag atctcctagt atcgttcccg
    34801 gctctgtcaa tggtggaaat aattccttcg agacaccgat tttttcaaga agttctattt
    34861 cccattcttt tgtccctgca ttcagcagct gcatagtaga tgcattagtt ttctccgtca
    34921 ccatgttacc agtaaagaca taccctaaat aatcaggtat cagtaacaat ttatgtgctt
    34981 ttgccaaatt ttctttttct tcagcaaaca attggaacaa ggtattgaaa ggctggatct
    35041 gaatccctgt ctgctcatac aacttatcta gagaatagat ttcagaaaac tgttctactg
    35101 ctccattggt acgcttgtcc cgataagcaa tcggatcatt tatccgatcc ccttgttcat
    35161 caatcagaca gtaatcgact gcccatgtat cgatccccgc aaaaaggcga tcaatccctg
    35221 tctgctttaa tttttcaaat ccaatcaaaa tctctttgat aagataatcg atcttccagc
    35281 gatcgtgacc gtctttgttc cgaaagccgt ttttgaaccg gtgtacttct tccaagatca
    35341 aagaaccttt tgcatccctc gtacttttca ttagtcgtcc gcttgatgca ccgatatcga
    35401 tggcaacaaa tgttggattc atttctccac ctcttttact ttattgttga ttagcacttt
    35461 attaataaag cgttatcatt tattgacaat gctatcataa tacagaatct tatattttgc
    35521 ttcacactat tttggtatat aattgcatta tcttgacaag ttgggagtgt ttacatggat
    35581 tactgggaaa aagccaacca ctcatggtcg gaagactcaa aaagagtgat cttgacgcct
    35641 acacaaagaa gcaggaattt atttttttat atccaagaaa tcggttactt caaagcacca
    35701 atcccttact atactgaacg agcacattta ccttcttact tgataaagtt tactttgtct
    35761 ggcaaaggtt cgcttacata taatgataat cagcaacagc taaaagctgg agacttattt
    35821 tttattgact gccaagaata tcagcgttat caaacgatca gcgaagaacc atgggaaatg
    35881 gattggatcc atttgaatgg tcagaacgtc caatcatttt atcaggagtt cacaaaagac
    35941 ggccataaca ccttccattc aacaggagat ccatcagaaa acaagatcca ccatttgatc
    36001 acagaattga tccacatgca acaaggcacg aatgcccgta ccgatttcca gtcctctgta
    36061 gctatccacg agttgctgaa tgaactgatt ttgcaaaagt atcagctaga tttcgaagaa
    36121 gaggatattc ccgcctacgt ttacaaactg aagcaatact tagacgaaca tttcaaagaa
    36181 acgattacac ttgatcaatt agaacaacaa ttcttattga ataaatatca gctgagcaaa
    36241 gatttttcca aatttatcgg ttctccacca atcgattatt tgatcagcaa aaaactttct
    36301 tacgctaaag accttcttcg gtatacagaa tttacggttc gaatgatttc cgaagaaata
    36361 gggattgaga attttgctta ctttagccgc ctgttcaaag caaaaacagg actatcgccg
    36421 agagactttc gtaaattcag ctgattcata taatatgtac gtcatttttt caaaatattt
    36481 tgaaatatat agataaaaat aaaataaccg cagtttctgc ttctatgtaa acaacagcag
    36541 ctactgcggt tattttttag tgatcattct cattgttcat cttcaaaatg ttcataatga
    36601 accaactgtt tctgtgtatc acgtttcaaa aattctttag gcgttatgcc gaactctctc
    36661 ttgaatgctt tcagaaaatg cggataagaa gaaaaaccac ttttactata tacagcacta
    36721 gagctgcggt attctcgcaa caagtgcttg gcatagatca gctttttctt cattacatac
    36781 tgatgaaacg taaatccagt gtacttttta aattctcgtg tgatatggta tttgctgaca
    36841 aaaaactgct tttccatttc ctctaaagaa agcggatgag ataaattttt tgagatatat
    36901 tgtaacattt gttccacacg ctcattagga actacagcat tctgttcttg cgcactctct
    36961 tctttttgta caacttgatt gagaaaaatc aagtattcta ccagagcttg ttcatatttt
    37021 atgtcttcac cgtaaatttt tttatcagga ggatggtcga taaatgataa tttttgcttc
    37081 aatacttcct gttctatttg caatacacga ctcctcttct ctcccattgg ttgaaaacaa
    37141 gccgatagat tagaccatgg cgtggaacga ctttctagaa aagaaggggt gacaaagata
    37201 tagacacgct cgaatacatc tgaagattgg cggacgatac gatgcaaatc attggaatgg
    37261 atgagcagca cacttcctgc tttgatcgta aattgtcgtc cgttcaaata aaaagtcgct
    37321 tctcccttta atgtagcatg gatctcatag aaatcatgat gatgatagat cgttttattt
    37381 tggctgacga catctttgac atgatagatt tcatactgat cagaaaccat cgtattgact
    37441 tgttcaccgc tgattttctt ttggagcatg tgccgcacct ccttttgttc acttatgcac
    37501 aattttatta taccacgttt tttgtaatca aaaagagctg cttttatata ctcttataaa
    37561 aaagaagaga cgtggcatct tctagccccg ccccctccat tctaaatatt agttaaccaa
    37621 cgtcttcttt acattattca agaagacttc ttccataacg aatcccatca tcttttgttt
    37681 cttcaagaac cacatacagg tacggacgat tcttcatgat gatttctttg atttttgtgt
    37741 actcgatcac accgtctccc aaatttaccg gcacgatttt ttcctgttcg acacgaaaat
    37801 ctttcaaatg aaccgcacat atcttttcac caaaacgttc aaaagcttct tctaccaatt
    37861 ggacttgttc ttgataatca ctacttgtga tcaaattggt cggatctaaa atgattccta
    37921 gatagtccga atctactgct tggatcagct gctctaccct tgctaaagaa tacaccggat
    37981 gattcaaacc tggttctatc cccaccatca tttggtattt ttctccagct tttaccaagc
    38041 gacggataac ggacacagct tcagcaaatg cttcatctgt gaaattttct tccgtatatt
    38101 ggatttccgg caaaacacat ccagtttccg aagcaaccat cgacgcacca aaggaagaag
    38161 catagcgaag atactgctcg aactttgtca gcaactcttg tcgtatcgtt aaatctggat
    38221 gaatcatatt gatatagcaa cttaaaattc ctacggtcac ttcttgcttt tctaaaactc
    38281 gtttgacata atttcccatc ccagaattga tctcatttgc gccagaaggt atttcaggaa
    38341 acgaaagacc taaggcgagc tgcagtgtat gaatcccttg ttcttttgtt ttttctgcaa
    38401 gatcttctat cgtttggact tcggaaagat catgtcctct aattgtaatg ttcaacataa
    38461 ctattctcct aactcagcat attgggtagc cacatgacga tttgcgggaa gtaagtaatc
    38521 aaaaacaaga cgactaagat aaccccataa aaaggaagaa gcggtttcag cattgcctct
    38581 gcttttacgc ctcctacact tgcaccgaca tatagtcctg tacctactgg cggcgtgatg
    38641 gagcctacac acagattcat gattgaaaac aacccgtaat gtacaggatc aacccctaca
    38701 cttgtgacga ttggtaaaaa tattggtgta aaaatcaaga tagcaggcgc cacatccata
    38761 aacataccca ccagtaataa cacaagattg actaataaca gaataataaa tttattatcc
    38821 gttacgccca taatcaacga actaattgcc tgaggaatac cggtaaacgc catggcgaaa
    38881 gacatcatgg aagaagtagc caataagaac atgatcgtcc ctgacattgc tactgattga
    38941 aacaacatat taggtatatc ttttagtttt accgttttat agtaaaacat agcaatcaac
    39001 agggagtaaa tgacagctat cgctgaagct tctactgctg taaatatccc cgtcaagata
    39061 ccaccgataa tgatgacaat caatagaagg ctaggaatcg cctcttttat tatttttcca
    39121 gcatgagcca tctgtccttt ttcgacaaca gggtatttat tctttttagc aacgatgaat
    39181 gctactagca tgattcctag cgcccacaaa gcgcctacga gataacctcc cataaacaaa
    39241 gcagagatcg aagcaccacc agcaacaagt gaatacatga taaaagcagt acttggagga
    39301 atgaccatac ctgtcggagc tgacgcgata ttgaccgctg tcgcaaactt ccggtcatat
    39361 ccttcttcca cttgctgagg gatcaacact ccgccaatcg cggtagaagc ggcaatggcc
    39421 gagctagaga tggaaccaaa taaagcattc cctaacacat tcgtatgagc caaagccccc
    39481 ggcacgcgcc cgacgaacaa catcgcaaaa tccactagtt tcttagctat ccctccatta
    39541 ttcatgataa tgcccgagag aacgaaaaac ggaatagcga tcagcgaaaa actattgata
    39601 cttcccacca ttttttgagc tgaactaaaa gcagaaacat caaacggtat aactaacaat
    39661 aaagtcatca tcgatgagaa tgcaatactt atagcaatcg gcatccctaa tacaagcaac
    39721 acaacaaata caaaaaataa cactaatccg gcttctattg ccattttatt cctccatttt
    39781 tatatttttt cttgaattgc tggcttgttg cttaatgtat cgattagatt gatcaggcta
    39841 tagataatga tgagtacccc cgatacaggt actgacagat aaacatatcc cataggaata
    39901 cttaaagaag gcgagatctg ccccattgtc aatgtgacag cattccatcc gccatacacc
    39961 atgatcatcg cagcaaatac cagaaataaa ctttgaacaa caacttctaa aattttactt
    40021 tttttgccag ttaatttttc acttaaaacc gttacggcta aatgtgcttt ttgtcccaca
    40081 acatacgcgg aagacaacat ggcaaaccag acaagaccga atcgaacaag ctcttccgtg
    40141 accgtatttg gatttcctaa aaccgttctg gaaaatactt gccataaaac aacagcgacc
    40201 atcccaacaa gcaaaacagc tcccaggatc tctaatacct tgtttaaaat cattttcatc
    40261 acagctacgc tccttgctct tggatctttt gataaaggtc tttgtaggta ggatcgtttt
    40321 tgaatatatc atgaagtggg gcaactgcct ctcgaaacgg tgtattatct acatcattga
    40381 attggactcc atgttcttct tgtgctaaag ctttttcttc ttcaattgct tttgcaaaag
    40441 cttcaatttc aaaaaccgtt gattctttcg ctgcttcttc aatggctttt tcctgctcat
    40501 cagttagctg ttctttgatg gcatcattca tgatgatgat atctggcaca cgtgtatgct
    40561 catcatagga atagtattta gccacaccac catgtccagc tgtatacaag acaaattcat
    40621 tgttttctga tccgttgatc aaattggatt gaagagacgt gtacacttca tcacttccca
    40681 taggtacggg cgaaccgcct aaaagctcta ccattttgat agcggtttcg ctttgcatca
    40741 cacggatctt ctttcccttc aaatcttccg gcgtatggat cggaccgtct accatataaa
    40801 agctgcgctg accagaatca taataggtca atccggtaaa tcctaaattt ttggtcgatt
    40861 ggtagattgg ctccattatt tcttcattat ccatcacctt aaagaaatgt tcttcatcat
    40921 tgaatagata aggaactgaa aaaatcgaat aatctttaga gaaactttcc aacgctgagg
    40981 cacttacttt tgtgaaatct actgctcctg actgtgtcaa ttcgatcagt tcacgttctc
    41041 cacccagttg tccatctggg aaatactgga tctgtacgga accatctgtt ttttctttta
    41101 ctaaacgccc aaattcattc attgcatcaa ttactggctg gctgttgctt gcataagcaa
    41161 agcgcaaggt aactatctca tttacatctg catctgcgac agttgccaca ctagaattat
    41221 ttccgcagcc gcttagacct agcagaccta ggctaagcga acttgctgca aacagcagtg
    41281 atgttttttt catatgggtt tcctttcttt taacaataag agacgagcaa ttctgtcaaa
    41341 caaagcaccg ttaacgactg tccataaggc attgctgtga ttccgatatt ccgataaaaa
    41401 tctaaactgt cccccattcc tgtcccgatt gaaacgtttt gaacttctcc tttttcatcg
    41461 atttgttcaa gcaatccttg gatagcacga taagcgactt ctttatattc ttgtggtaaa
    41521 taacgtttat ggaccgcttt caaaataccg taagcaaatc ctgcagttgc agaagattct
    41581 acgtaagaag aaggatcatc caacaacgta tgccataaac cgctttcaga ctgataagtt
    41641 tttaatgcac gcacttgtgc ttcaagtgtt tcgatcaata atttgcgcaa tgcatcttct
    41701 ttcgataatt ctaagatttc aatgatctct ggaatagcaa tcgtcaacca gcagtttcca
    41761 cgagcccaaa atgcttcggc atagttatga tttccttcaa acgtccagcc gtgataccac
    41821 aatcctgttc gtttatccat caagtatttg atatggatca taaactggta acgagcttct
    41881 tctaaatatt ctgggcgatt caacagtttc ccgatttttg ccaaaggaag cactgtcatc
    41941 atcaatgtat cgtcccacaa ctgattctta ttttccggtc catatgtggc atgctgtaac
    42001 ccgtcttcat ttgtccgagg catgtcattc atgacccatt ctgcccattg ttctaaataa
    42061 ggcaaatagc gtgaatcttt cgtgtcttca tacaagtaag ccatagtcaa tagcggtgcc
    42121 attgtattga cgtttttggg cggagcgcct tcttccatac gatcctcaaa ccattcattg
    42181 accacttgat aagcgcgagg attttttgtc atctggtaat ttttataaat accatataga
    42241 ccgacacctt gtggccaatt ccacactgtc cagcttttgt catctacttt cagtccatca
    42301 aagtccaaca aaaattcacc attatcgtct ttgatcgttg tcaggttttc aacgagcagg
    42361 tcaatttttt ctttcactaa ttctttaggt acaaaaatct cttctttaat aatcatcttt
    42421 acgccacctt tttaaacgct ttcattttat atccttaata tagcagtaat accgtccact
    42481 ataaatatcg ctttttgaac aaaaactgtc taaaattgac ctaaatgtta aataacaaaa
    42541 gtaactttca gttacttgga aattaaaaaa agaacttgga gaaacaacgt tttccaagtt
    42601 ctttttcatc tattttctta tcttttttct acgctttctt ttgcttatcc gttgtaagca
    42661 tcaatgatga tctgtttcaa ttcagagatc aatggttctt tcggattagc tgtcgtacat
    42721 tggtcttcgt aagctaattc agccatacgg tctacggttg tatctagtgt ttcttgtgtg
    42781 actccttgag cttttagact catgttgatg ccgacagatt ttcctaattc ataaacagcc
    42841 gttgctaaag cttcgactaa ttcctctgtt gtttcgcctt tcagacctaa gaattttgcg
    42901 atatctgcgt aatctgtatc tgcacggaaa tagtcatatt tagggaacat cgcatgtttt
    42961 tgtggatctt ttgcattata gcggataata tgcggtaaaa gaatcgcatt tgtccgtcca
    43021 tgagggatcc cgtattcccc gccgatttta tgtgcgactg agtgacaaat acctaggaat
    43081 gcgttagcaa acgccatacc agccattgta gaagcgttgt gcattttttc acgtgattcc
    43141 atatcaggtg tttttactga tttttctaaa tagtcaaaga ctaacttgat tgcttgtagg
    43201 ctcaatccac gtgtataatc tgaagccatg actgaaacat aggattcgat tgcatgtgtt
    43261 aacacatcca tcccggtatc tgctgtgaca gatgctggaa ctgacatgac gaattgagga
    43321 tcgacgattg cgacatctgg cgtcaatgca taatctgcta acggatattt cacatgtgtt
    43381 tcactgtcag tgatgaccgc aaatggcgtt acttcagaac cagtccctga tgttgtcggg
    43441 atacatacga attgtgtttt ttctggtttg tcgatcttat atgtccgttt acggatatcc
    43501 aagaattttt gttttgcacc aaagaaagat gtgtctgggt gttcatagaa catccacatt
    43561 cccttagctg cgtccattgc agaaccgcct ccaagagcga ttactgtatc tggcttgaag
    43621 tctgccatca gtttcgttcc tgcgtatacg gtatttgtcg atgggttcgg ttcaacatct
    43681 gagaagactt cgatcttcac atcattttta cgtcgtgcca atacttcacg aactgtatcg
    43741 cagtaaccga attggaccat accagggtca caaacgatca tgacacgttc aacgttttcc
    43801 attttctcta agtacaatag agagttcttt tcaaagaaga tttttggcgg taatttgaac
    43861 cattgcatgt tatttctccg tttcgctaca gttttgacat tgattaagtt tacagcagag
    43921 acgttacgag aaacagagtt cttaccataa gaaccgcatc ccaaagtcaa tgacggaatc
    43981 atttcgttat agatatcacc gatgccgcct tctgcagatg gtgtattcac taaaatacga
    44041 caagctttca ttcgaagacc gaatttgaca tgcaactctt cgttttctga atggataact
    44101 gctgtatgtc ctaatcctcc aagattcaac ataccttcac acaaatcaaa cgcatggtcc
    44161 gtgttgttag ctttgatcat ggcaagaact ggagaaagtt tttctcttga tagcggataa
    44221 tctgggccta cgccttccaa ttcagcaatc agcattttcg ttccttttgg tacactgatt
    44281 cctgccaatt ttgcgatttc catcgcagaa tgtccaacga ttgatggatt gaccgcatat
    44341 tttccttcgt tcatcactgc atcttctaat ttttgcaatt catctggttt tacgatataa
    44401 acttggtgtg cttggaattc tgcttttact gctgcataga tttctttatc cacgatcaca
    44461 gcttgttcag aagcacaaat cattccatta tcaaatgttt tcgaaacgat caaatcgttc
    44521 actgcacgtt taatttttgc tgttttttca atataagctg gtacgttacc tggacctaca
    44581 cctaaagctg gttttcctgt agagtaagca gacttgacca ttccagctcc acctgtcgct
    44641 aagacgatcg cgatacctgg atggttcatt agcatagaag tcgcttcgat cgaaggatgt
    44701 tcgatccatt ggatacaatt ttcaggagct ccggcagcga tagcagcatc acgtaccact
    44761 cgtgctgctt ctgcagaaga tttttgggca cttggatgga aagcaaaaac gattggattt
    44821 cttgttttca acgcgatcat tgatttgaaa atcgttgtgg aagtcggatt cgttgttgga
    44881 gttactccgc aaaccacacc gactggttca gcaatttcaa tcaacccttt ttggacatct
    44941 tcattgatca caccaactgt tttatcatgt ttgatattat gccaaatata ttcggacgca
    45001 tacatatttt tgattgcttt atcttcgtaa acaccgcggc ctgtttcctc tacagccatc
    45061 ttcgctaatg gcatatgttg atccaaagct gccattgcca tttcatgcac gatatggtct
    45121 actttttctt gatcaaagct ttccatttct ttcaatgctg catttgcttt ttctgccaaa
    45181 tcatcaatca ttgcttgtac gtcaattgtc ttcgtttttt ctttttctat tactttagcc
    45241 atcattatcc tcctaatggg tcatggttat ttgttaaata attcacatgt atatcataca
    45301 atatttattt ttctatgtaa acactttttt gtgatttttt tcacagttat ttttaaatat
    45361 aaaattgctt tatttacagg tgtttgtata gaaaaatata taaagttatt tattattatt
    45421 tatatgacac acccgattat tcaatattga gcaatatatc ccatgctctt atctctttct
    45481 tttttgaata aaaaatgagg taggacatct ttctgcccta cctcgtcttt aaataagctt
    45541 tgatacttat ttttcttcct tctcttctgt ctcttccagc gtttctgttg tttcagtttt
    45601 tacttcaact gtctcagctg gttcttctgt tgtttcaaca gtagtcgttg catcatttgt
    45661 tacgacagtc cctggtttga ccgttttgat tgctgcacga tcgtactcaa ggaagatccc
    45721 ttcgcagtct aaaacaactg tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa
    45781 tccgccgatc gttacgactg aatcacctgg tttcatgcta tctaacaagt tttgacgttc
    45841 ttgctgttgt ttcttttgcg aacgtgacat aaaaaaccac attgctaata gcgcgataaa
    45901 catcacaagc attggtaatg ttcccatttt tttcacctca ctaattctgt atctacaaaa
    45961 aacactttag cagaaaaatg ctgataacac tagtttttta cttaattttt aaaagatttt
    46021 cctttagaag ctttttgcgt tttctttatt gaacccgtat tcttcaaaaa aggcttctct
    46081 aaattcaagc aaattatcat ccatgattgc ttgacgtact tgtttcatta gattcaacaa
    46141 gaagtataaa ttgtggtaag aagtcaaacg aatcccaaat gtttcatcac atttgatcaa
    46201 atggcggata taggcacgtg tatagttttt acatgtataa caatcacatt tctcatcaat
    46261 tggacggaaa tcatgggcat atttcgcatt tttcactact agacggcctt gagaagtcat
    46321 acatgtgcca tttcgagcga ttcgagtagg caagacacag tcaaacatgt ctactccacg
    46381 gataacacca tcgatcaatg aatcagcagc accgacaccc atcaagtaac gtggtttgtt
    46441 ttcggggatc aacggtgtag tgaattctaa tacacgattc atttctgatt taggttcccc
    46501 gacagataac ccgccgattg agtaaccagg gaaatccatc ccgactagat ctttcgcgct
    46561 ttgacgacga agatcttcaa accctgctcc ttggatgatt ccaaataatc cttgacggtc
    46621 tggattagca tgagctttca atcctcgttc tgcccatcga gacgtacgtt ctactgattt
    46681 tttcacgtag tcgtaacttt catcgaatgg tggacattcg tcaaaactca tcatgatatc
    46741 agagcccaat ttattttgga tgttgattgc tttttctggt gataaaaaca tacgcgaacc
    46801 gttcaagtga tttttgaaat gcacgccttc ttcttcgata ttccgcatat cacttaatga
    46861 aaaaacttga aagccgcctg aatctgttaa aatcggttga tcccagttca taaatttatg
    46921 cagtccgcca gcttcttcta ctaagtcttc accagggcgt agccataaat gatatgtatt
    46981 acttaaaata acgccagcac ccatttcttt caactcttct ggggacattg tttttactgt
    47041 agctaatgtt cctactggca tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa
    47101 ttcccctaaa cgcgcgccag tatgtttttc tttcttgatt aaacgatagc gaatggcagg
    47161 ttgcgtcatt catttcccta ctttctatat aaaatatctt gacaactccc accatcataa
    47221 tcgtttcaag tgctttttgc aagttccctc ggcttccttc tactttattt aatattcttt
    47281 tttttatcct ttttttatga ataacaaaaa aaagaaaaac attatgacag aaatattttt
    47341 tgatacatgg tatattggtg ttattaaaga aaaggaggga aaaatcatgc aatcttctgc
    47401 agaattaaaa cgccaagcaa aagagagtct aaaaggacgt tggggacaag ctgttttact
    47461 aaatctaatc cctacattga ttggtattgc tactattctt cttattatga ttcctattat
    47521 cttgtttgcc accaccatat atgatggatc agcgagcgtc tcagaagcga caacttcagg
    47581 aggcggtagt tcaggaggcg gtggcggaat tatttccagc atcattagcg ctttgttcat
    47641 gagcggtatt tcttggactt atcttgatat tttacgtgga caaaaagatc agatcgaacc
    47701 attcaaagat gcatttcgtg gattcaaagg tttgtttatc ggcggagtga tcagcttagc
    47761 tgtgctgatc tcgatcttta caacattatg gggtttgcta ttagttattc ctggaatcat
    47821 taaaagctat tcttattctc aaagctattt tatttactac gatatcgtcc aagaaacagg
    47881 acaaacacct aaagtactgg atacgatcac tgcaagtcga aaattgatgg atggttataa
    47941 aggacaactt ttctggttag atctttcatt tatcggctgg catatcttag cattagcaac
    48001 tgctggtatt ggttatctat ggctcagccc atacatttct gcaacaaaag cagcatttta
    48061 tgaacaattg ccaaaagata tttaaaagag agtgtgactt gtgaccgagg gggtggcagg
    48121 agaacaccgt ttatcgagag gcagtaaaag aaacattcag cccattagca aaaaggaggt
    48181 gtgacaacat tcatgtcaca cctccttttt gtatagttcc agtaaaaaga aaatcaactt
    48241 agaaacagtt aatcattcct cttcttctgc ttccggtaat cgatggaact tgatcagttc
    48301 attcaccgcg attggtacga tagataaaat ccctaccaag aaccagtgat tcaaactgat
    48361 gtgtactagg ccgaatagtt cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc
    48421 taatgccagt aacgccattt ctaccaaagc tttattggtc gctaaattga ctttgaagat
    48481 cgattggctg ctgcgtacat tgaacacatg cagaatcgat gaataagcta agatcaagaa
    48541 tgcaacagtt tgaccgacat gctgacttgc gtctacccat gggctgatag tatcgacata
    48601 actacctaaa taataaccaa acaaggttac gatggtgaag acccctgcat ttatggcaat
    48661 tttttgcaat aaacctcgag caaagatccc ttcatttttc ggaatgggtg gctgatccat
    48721 gatgccgtgt tccgctggct ctcttcctaa agcaaatcct ggaagtccat cagcaactac
    48781 attgacaaac agtagttgca cagctgtaaa tggtgctccc catcctaaca gcatcgcaat
    48841 caaaacaata aagatctccg aaatattaca gcttagcaaa aagttgatag ctttacgaat
    48901 attctgataa accgaacgtc cttgactgac agcgtctacg atcgtcgcaa agttgtcatc
    48961 cgtcaaaatc atatctgcgg cactttgcgc cacatctgtt cctgtgatcc ccatggcaca
    49021 cccaacgtta cttgctttca acgctggtgc atcattcaca ccatccccag tcatcgctac
    49081 gactgcaccc gtacgttgcc aagatttgac gatccggatc ttgtcttctg gcgttacccg
    49141 agcatagaca gagagagctt tgacacgcgc atctaattct tcgtctgata attgatgcag
    49201 ttctgatcct gaaagtgctt ctgacggatc attcaagata cctaattctt tcgctatggc
    49261 actggcagtc accacatgat ctccagtgat catgactgtt ttgatgccag cctttttcgc
    49321 tcgcttgatt gctcctttac tttctggacg tggtggatcg atcattccga tcagaccaat
    49381 caaacgtaag tctttttcca gtgcttcaga agaaatatct tgcggttctt cgtcataagt
    49441 agcataacca acagcaatca ctctcagtgc ccgtttacca aagcgatcat taacgatcgc
    49501 cgcttgttct acatcgcctg aactaaagcg cggagcaaga acatcgaatg ctcctttggt
    49561 gattgagatg tatttttcgc ccatttggtg aactgttgtc atcatttttc gttcagaatc
    49621 aaaaggaagc tcgtttactc gtggatattt ttcttctaat tctgcctttg tatgataatt
    49681 ctcttcaaca gcccttacga tggctacttc tgttggatta cctttgataa caaggtcgtc
    49741 atcttcttgc tcgaccgtca catctgtaca tagtgcagcc attttcaata tttccatcgc
    49801 gctgttcgtc attgaatctt cgatattcgt tacttcatct tctttgctcc atacccggcg
    49861 gacgcgcatt tcattttgtg tcagcgttcc tgttttgtct gagcagataa cattagcggt
    49921 tcctagcgtt tcaactgctg gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg
    49981 cacaccgtat gcaagtgtca acgtaacaat aacagtcaag gtttccggta cagctgcaac
    50041 agccaaggaa atcgaagtca tgaacatctc aaggagtggt tctccttgca gttcaccaat
    50101 cacaaatact aaagccgcag ccgctaaagc gatcatactg attcgtttgc ctagctgatt
    50161 caatcggacc tgtaaaggtg ttttttgcat agtattttcg ttcaaaagat ccgcgatctt
    50221 acccatttct gtagccatgc caatggcagt gacgatcgct ttcccacgac ctgcagcaac
    50281 cgtacatcct ttaaatacca tattaagttg gtcacctaat gactcatctt cttcagcaat
    50341 aaatgctgca tctttttcaa ctgcttcact ttctccagtc aacgccgatt cttctacacg
    50401 taattgtgat gcttcgatca aacgggcatc ggcagaaata gcatcaccgg actctaaaac
    50461 aagaatatca cccactacca gttgctctga ctcaacagtg gtcactttgc cgtctcgtat
    50521 gacagttgcc gtttgtttat tcatatcttg caaggattcc agtgcttttt ctgcgtttcc
    50581 ttcttggaca atcgccaata ccgagttgat cacaacgata gcgatgatca gcagtccttc
    50641 aaataaatcc ccatgttctg tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag
    50701 aaggatgatc gttgtaaaat cggacaagct tcgaaagaat tttttgatca tcgattcttt
    50761 cggcgcctca tcgaatttat tcaatccctg cttttttcgc cgttcatcca cttcttgtga
    50821 agacaaccca gtatctggat ctgcttctgt taccttcttt acttcttcta ttgtctgctt
    50881 gtattcttcc atcctattcc tcctttaaaa tcttcgtaca aaaaaaggta cttattttac
    50941 tataacatcc tttttttatt tacttcaaat aaagggctta tcgattattt gttaagaaag
    51001 cgtaaaattt aaagtgctta tatttgcaaa cgctaccaaa ggatgctatt atatagatgt
    51061 aaggaaggag gataccatca aatagacaga agaatttctc gatagaacag tgtaagcaaa
    51121 caaagttgtt agaagtacag gtttcaccca gacatgcgtg tagagtacaa acctataaat
    51181 agtgaatgcg tatggatgtt tggatcacga agtatccgaa acaagaagta atgcgatacg
    51241 taacaattga ataacgtaaa cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt
    51301 tgaagatgta acgaaaacaa aatttttgtt taacatgaag aaattcaaca ccgtgctttg
    51361 catgtatgat ggaaagttac aatcatttct gtttatttga atccttccga ggtacaagaa
    51421 agccgagctt atcgagagct cggctttttc tttgtcataa aatctttgta aagctttttt
    51481 gttattcaaa actagcggct tttcataacc ttatttcacg aacatcgcat ctccaaaact
    51541 aaagaaacga tagcgctctt caatcgcatg ctggtaagca gataaagtca attctcttcc
    51601 tgcaaaggca ctaactaaca tgactaaagt cgattttggc aaatggaaat tagttgagaa
    51661 agcttctacc actttgaatt catagccagg cgtaatgaaa atatcagtcc atccgctgtc
    51721 tgctttgatt tctccattaa atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt
    51781 tccgacagcc acgattttcc cgccattttt acgtacttgg ttcaatcgct cagccgcttc
    51841 ttccgtcaaa cggtagaatt cactatgcat atggtgttct tcaattttat ctacgctgac
    51901 aggtcggaat gtacctaacc ctacatgtag tgtcagatag actaattcaa caccctttgc
    51961 ttcgatctca tcaagtaatt ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga
    52021 accattttct ttcgcataga cagtttgata gcgatctggg tcttccagac gttctttgat
    52081 atatggaggc aatggcattt ctcccaatga ttcaagaatc tctagaaaaa ttccttcata
    52141 ctcaaagtca atgatccgtc cgccgtgttc caattcttct ttcactactg cttgcaatct
    52201 gccatcacca aaagagattc ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt
    52261 ctcccacgta tctccttgtg tattcgttag aagaaggact tctaaatgcg caccagtatc
    52321 tggtttttct ccatataaac gagcaggcag aactcgtgta ttattcataa ctagcgcatc
    52381 accaggctgt aactcttcaa tgatttcatg gaaatgttta tcttccatct ctccagtttt
    52441 ccgatccaaa acaagtaaac gagaatgatc gcggtctttt aatggtgttt gtgcgattaa
    52501 ttcttccggc aagtcaaaat caaaatcttc tgttgttaac atatcgtcac tcactttcta
    52561 tcatgtgctt catcattcta tcatgttttt cgttctgttg gcaaaatcag cactttttca
    52621 cttctatttt ttgttttcct aaataaaaaa actaccctat ttgtattttt tcattgtttt
    52681 aggcatacta gtaatagata gttcttcatt gaaaatagaa aaagaggtgg caccatgatt
    52741 gaatttcaaa atgtgtcaaa aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat
    52801 ctttcatttg aaaagggtga gttcatttgt tttatcggta cgagcggaag cggaaaaacg
    52861 acgacgatgc ggatgatcaa ccgaatgact gatccaacac aagggaaaat tttgattgat
    52921 ggaaaatcca tccaagaaat tgatccagtg gaattgcgac gtcagatcgg ctatgttatc
    52981 caaagtattg gattgatgcc tcacatgacg atccgtgaaa atatcactct tgtccaaaaa
    53041 ttgttgaaag tcagtcaaga agaacggaat aaaacagctg aaaaaatgat tgatcttgtg
    53101 gaattgcccc gtgagatgtt agatcgctac ccacatgaat tatctggtgg tcagcagcag
    53161 cgtattggag tcgttcgtgc ccttgcagcg aatcaagata tcatcttgat ggacgagcca
    53221 ttcggtgcgc ttgatccaat cactcgtgat tccttgcaag accttgtcaa agatttacaa
    53281 gagcgtttgg gaaaaacgat cgtatttgta acccatgaca tggatgaagc gattaaacta
    53341 gcaagccgta tcgccatcat gagtgaaggc cgtgtgatca atttgataca ccagaaaata
    53401 ttttacgtca tcctgcaaac gatttcgtag aagagttgat tggagaagat cgcctactac
    53461 aagccaagcc aaatgcgaca agagtcggcg aagttatgct aaatacagca atcacgatta
    53521 caccggaaaa atcattacaa gaagcaatca aactgatgag ggaaaaacgt gtagatacgt
    53581 tgttagttac cgataattcg aatgtattaa aaggatttat cgatgtagaa acgttgaata
    53641 aaaaacgagg aaaagtatcc agtgtcggcg acattttgaa taaacatgtt ttttacgtaa
    53701 aagaatccgc ctatcttcgt gataccttgc aacgcatttt aaaacgcggg ctgaaatacg
    53761 tgccggtcgt tgatgaacag aacaaagtcg taggtatcct gactcgtgca tcattagtgg
    53821 atatcgtcta cgatgtgatt tggggagaag atcccgcaac tactgatgtt gcagagtcag
    53881 ctactcctga agaatctaag gaggtctgac ctatgcagca gttttttcat cagcacggtt
    53941 cagaaattct ctcaaaaggt tttgaacatc tttatatttc aggacttgct ttgatcattg
    54001 gtatcatcat tgctgtaccg cttggtgtct tactgacacg ttttccaaaa gctgccacga
    54061 ttgtgattgg cttaaccagt gccttgcaga caatcccttc tcttgcttta ttggcattga
    54121 tgatcccgct atttggcgta ggtaaattac ctgcgattat cgcactcttt atttattcat
    54181 tattgccgat tttacgtaat acgtacattg ggatgaaaaa tgtcgattgg aattatcggg
    54241 atgtggcaaa aggcatgggg atgactgagt tccaatccat tttttccgtt gaattgccca
    54301 ttgctatgcc aacgatcatg gctggtattc gtttagctgc cgtttacgtg attgcatggg
    54361 ctacacttgc ctcttatatc ggcgctggtg gtttaggcga tttgattttt agtggactga
    54421 ataattacca accagatctc attttggcag gtacgatccc tgtaacaatt ttagcactat
    54481 tggctgattg gcttctaggt ctcttggaac accggctgac accgatcgca cttagagagg
    54541 agaatgaaga atgaaacgta tactaaaaat gaccatggtt cttttctctc ttcttatact
    54601 tgcgagttgt tccttccctg gtcttgccag caatacagat gaagacacca tttcgatcac
    54661 aggtggaatc acttcagaag cacagatctt agcaagtcta gttgccggaa tggttgaaca
    54721 ttatacagat aaaaataccg caatcatcaa caatctggct actacaacta tcaaccatca
    54781 agcgatgatg aacggagatg cttcaatttc agcggctcgc tacacaggaa cagatttaac
    54841 gactacattg aaccttcctc ctgaaaaaga tccgaaaaaa gcgtttgcta ccgtgaaaga
    54901 tgaatttgag aaaagatacg gtcaaacgtg gttcccgtcc tatagttttg aaaatacgta
    54961 tgtctttctt gttcgaaaag acaccgcaca aaaataccat ctatctaaag tcagcgatct
    55021 gaaaaatgta gcggatgaat tagtagctgg tgtagatact tcttggatca atcgtaaagg
    55081 agacggttat gacggcttcc aagaaactta tggcttctcc ttcaattcga tattacctat
    55141 gcagattgga cttgtttatg acgcagtcga agcaggaaaa atggatattg ttcttggcta
    55201 ctctacggat ggacggatcg ccagttatga tctagtcatg ttggaagatg atcgtcaatt
    55261 cttcccacca tatgatgctg ctccagttgt agacaacaag atattagctg atacgcctca
    55321 tttaaaagaa gccttaaata agttaggtca taccatcagt acggaaaaaa tgcaggaatt
    55381 gaactacgaa gcagataata atctagtgga accatcagta gtagcacaac gtttcttaca
    55441 agaaaaccac tattttgaag ggaagtgaag caattggata aaatgaatgt ctttcaacaa
    55501 tttttctatt atttccagga aaatggctcc tatatctttg cacaatttat ccgtcacttc
    55561 ctgatttcta tctatggtgt cctctttgca gcagttgtcg ggattccggt tgggatcatg
    55621 atttctagga gacgtaaatt agcaaactgg gtgattcgtt tggcgaatat catccagacg
    55681 atcccttctc tagccatgat ctccatttta atcattggat taggcctggg agtcaatgtc
    55741 gtgattgtta ctgtattttt gtattccctc ttaccgatca tcaagaatac gtataccggg
    55801 atgatccaag tagacaaaaa catccttgat gtcggcaaag gcatggggat gactgctaga
    55861 caacgactat tcatggtaga gttaccactc tctgtttctg ttatcatggc aggcataaga
    55921 aacgcgttag ttgttgccat cgggataacc gcgatcggtg cattcgtcgg tgctggtggt
    55981 cttggagata tcattatccg aggaaccaat gcaacagagg gcacatcgat tattttagca
    56041 ggtgcattgc caactgcatt aatggcgatc atcacggact ggcttttagg tattttggaa
    56101 agacggctag atccagccag ccgtacatca cgctgatgtt attgtaaaaa acaaaagctt
    56161 ctgcgaccat ttgttggtcg caaaggcttt tttagttctg tcatttttaa ggcaataatc
    56221 cactctgatt cttccctcct tatgggcaaa gagttgcttt tcttagctat ttatccattc
    56281 cgtatactcc aacttcatac tgccaacggg ttcatatctg catccaccga ctaagttttt
    56341 ttcttatccg ctaaggcatt ttgccaatcc attttcccat cactcgtcat ggaattcata
    56401 ccacatgatc tctaaatact cgtctgcttg ttctttgaat tgggcatcaa ttcttacatc
    56461 ctttggcgtg gctttcattt aagccttttc catcagtcct cgattgttcg atgccttcag
    56521 ctgcatcatc aaaactggtg actgccgtat cggtaacttc gatcatcttt tatacaatcg
    56581 ggaacttgcc atcctcatcc ttggctaaaa acatatctag ccattccagc ttctcgccat
    56641 gggaggtttg cggttcctaa aaccggtaca cgattcttat ggactgcatc cgtcgcaact
    56701 agacttggcg atacgatgat ccttcaccag aagagcaccc ccgtaaacca attgatcgat
    56761 atattgccaa taagaaaaga cattggcgta aaagatatcg atccctcgtg atgcattgcc
    56821 gttgatactg ctattcatgg ccgaaagcgc cacgacttta acttcttccg tttgagtgac
    56881 attcatgttc ggcaatgtcg ctttggatac ccgctctgct aaaggaacga cactggtatt
    56941 gtatttcgta tctgtatctt ttccaaaaga ccatgccaac gaatcctctg ggaaccaata
    57001 agacgactcc ggttgattgt ccattgcttt tttcaacata cttttggctg tttctcgata
    57061 aacgacattt ccaattgtct tcgcctcact ttggcgataa tcgatggtcg ttgccactgc
    57121 caacactaac aacgatgctc caattttact gattttcctt gtcacacttc ttcctccttg
    57181 ttttacataa acccgtcaac attccattcc taaactcaaa caatgaccct tcaaaggtgg
    57241 agatatagga acaaattaaa gaaatcatgc aataaatcca agaaggttgg ttttagaaca
    57301 aaaaaagagt ctcctttttg gggagactct ttgttaacta tctaaaaagg ttggctcttc
    57361 tcacttcact cataatgtgt ccaggcagaa taaatttcta caactttatt ttcctcatca
    57421 actttgtaaa ccactcgatg ctgtctattc agcctgcgtg agtatctgcc ttcatgtgta
    57481 ggtctcagct tttcaaaaga ctgcgtgggc atatatggat cttccttcaa tgtctgaatc
    57541 acttcttcaa attgtttctt taaattcgtt tgcttgattt ttcttaaatc cactttcgca
    57601 gagtttttga ttgcgactgt atagttactc atcaaagagc ttcccaatca atatcatcga
    57661 tattcgtaaa gctactgtcg tctttttcgc ggtctcgtac tttatctaaa gtacctgttt
    57721 gctcaagaaa aagggtttct tgaatcgctc tccagtcttc cagcccaatc aatacagcat
    57781 tgtttccact acgatcacta ataatctcga tttcagtatg attttcattt acctctttca
    57841 ataactgata aaaattttta cgagcagtgc taggattcat cgttgtatta gccattgtct
    57901 ctcctccttt ttctgatata attatagcgt acttgattaa gtacgtcaaa aaaatacaat
    57961 aaagcactta aattcagcaa acttttcctc ttttttacat agagaaaaag accccttttt
    58021 agggaggatc tttgtccacg ctcacttctt actcctttac gactagcggt agccaaacgt
    58081 aaagtaattg gtaacaacgt atcatcttta gcaaaggtcg aatccaaagc ctgtgagtct
    58141 atgcaacaac accaatacac gaaggttcct cgttgcagtg tgacttgatt gatatcttga
    58201 ataacctgag gatacgcacg aaagatttgt aacggttatt caaaactgat tttggccgtc
    58261 aagtcttcta ctgcctcaaa aatgattgcc tctttcgtct cctgtcattc atgttcacta
    58321 tcgatttgat acttgcttag accgttgggt ttgtgaatca ccaattaaaa atggcactga
    58381 gttatggtaa caaaaaatgg tcacttgacg aggctgagat tcctacttat gaagacacat
    58441 ggcacttaag agtcgaggta aaggaagaag ctgaaaatat aggaggctgt acattatttg
    58501 gcaaaggatt cggaaattat gatcaagaca tcaaaatcaa aatgttttac agctaaatag
    58561 gaagaataat aacatttcat acgctgttcc catctttaat ggcttataga gggcaccttt
    58621 agctgagaat aagacaaaac agaccctcta gcttcaatta agagctagag ggtctgtttt
    58681 tgattttaaa acttggttta tttcttcaaa tggtagctat aggtgctgac aacgatattt
    58741 tcacgccaag acaatcgcaa tcttaaccgc aaacttgttt ggttatggag aatattctgc
    58801 cagcgtctgt ttggtacaaa ttgaggaatc aacacagtag ttgtatagtt ttgtttcgct
    58861 gcatttttac ttacgatatc tacataacgg ataattggat tttcaatcga gcgataagaa
    58921 gagtggacga ttgaaaaacg gacatctggg aaatgctttt taaattctgc ttcgatttct
    58981 ttttcttttt cgacatcttc gtctagcgaa acatgcatcg caacgacata atcaccgatc
    59041 gaacgtgcgt aattcaacgc accgatattt acccgtgtca cattcccgac taatacaaga
    59101 acggtattcc cgtcataatc atgtagtttt gcatcatttt ctaaacgaag ctgttctgct
    59161 acttttttat aatgatcatg gatcttataa aaaatgaaca taacgattgg cataatgatg
    59221 aagaaaggcc aaatgtcccc taaccgatag acaaacaaaa tcgcaatgat tgcatatgag
    59281 atgaacgcac ccgtaatatt ggcaatcgat ttgcttaacc atctttttcc ttcttttttc
    59341 catttgacga ccatccctgt ttgagataaa gcaaatggaa tgaacacgcc gatagagtat
    59401 aacggaatca agcgttctgt tgagccatgg aaaataaaca gcaatacgat cgaacctagc
    59461 gctagtgtga taatcccatt ggaatagcct agtcgatccc cacgatcttg atacatgtga
    59521 ggcatgaatt tatcttttgc cagattgtat gcaagaactg gaaaagcaga aaaaccggtg
    59581 ttagcagcaa ctgctaaaat cagcgctgta gcaaattgaa ggacgtaata caaaatacca
    59641 tgtccaaaga cagctttacc aatttgagac aaaacagtga cttctttttc aggaacgatc
    59701 ccataccaat agttgataaa tgtgatcccc acgaagaaga aacctaagat caatgccatc
    59761 atcgtcaatg tagcagcggc attttttgct cttggcttct taaagaatgg tacagcatta
    59821 ctaatcgcct ccaccccagt taaagaggaa gaaccagaag aaaatgctcg taaaatcaaa
    59881 gcgatagaaa cacccggcac agccgctcct ggaagtgcag tagcttgcaa tggcacagca
    59941 cctgtcacga tattatataa accaacaacg atcagtaacg agatcacaag gataaatgtg
    60001 tacaccggaa ataataggaa cgatgcagat tccctcagcc cacgaagatt cagcatcatc
    60061 agtagcaaaa cgattgtcac agaaatagcg acttgatgac cgtagagtgc tggcaccgct
    60121 gatgtgatcg cttcagcacc tgctgagaca gatactgcta ctgtcaacat gtagtcgatc
    60181 aataaagagc caccggaaat caagccagca ttctttccaa gattctcact gctgacgaca
    60241 tatgctcccc cgccatgagg ataagcatgg atgatttgac gatatgatag cgttaatgaa
    60301 atcaataaaa taataacaaa agcggcaatg ggtagcgaat accagatggc tgcagcagac
    60361 aaagcgacta aaacgacaac gatttgttcc gtaccatatg caatagatga taacgcatca
    60421 gacgacaata atgccaaagc agcaaatcgg gttaatttgt gctcatcatt ttcagcagat
    60481 tttaaaggct tacctactaa caaccttttt aagtaatcca atttctaaac tcctttacac
    60541 acaattttac ttcaagtttt atagtcgaaa atttatagta aacaagatgt tatagtacgc
    60601 tcactgacct taaaagtcaa taaactttaa gaaaataatt ggaaaaaaac agaaaaatat
    60661 caagcgcttt ttcaaacgct ttaatacagc aaaaatctaa cattttttta tgggcttttt
    60721 ctttgaattc tataataaaa ggatagaact tgacttagaa aatcaagttc tatccttttt
    60781 gatatcttaa cttttggaag agagatgtaa ggctgctttc tacccatcat tcttactact
    60841 cgaagcatag cgcagttgtc acaacctctt gataaacggt gtttaaaagc ttgcatctgc
    60901 ggtaataagc ccaaccaaat taaaaacata agaagctgtt tttatttggt cgttcttatt
    60961 acttgatgca gagcagcttt tttacaacct cttggtacac ggtgttcaat cagctgaccc
    61021 tcggtattag ctcaaaaaaa cggcaaaata tgaggaacta tttttccatt tttttctcta
    61081 atactcaggt caaaacactg atttcacaac ctcttttttt aatgaaaaat atggctctca
    61141 tgcgccgctt ctttgaattt cgtcattttt tcttcgatga acggatagat tgccgttcct
    61201 acgattccaa agacaacaaa caaaccgatc atgatccaga ttgctttcgt agcatttggc
    61261 caataaattc ctcctgcaga ttcccgtaac aagtttaccg catgagtaaa tggcaagaac
    61321 ggattgatca tttggaagaa tttccctgag acttggatcg gatagttccc gcctccgcca
    61381 gagatcgaca gtactaagat gataatagcg atccctttac cgacattatc gaataatgca
    61441 gccaagacat agacgatcat catgaacgct aaggcgatca agacagcaaa tgacacgctg
    61501 tagaccggat tttggacatc tactcctagt aagaaaatat tccctaatgt aacgatcaag
    61561 ctttgcgcaa tcgccattgt caaaaatgtc agcatacgag caacgaattg ttctcgtttc
    61621 gtaaaattgt ttcggtcttt ctttcctaaa tagtattctg tcgtagcaac acttgaaaga
    61681 agaacagcgc cgacccacag acatagtgca gtataaaacg gggtgctagc tgaaccgtta
    61741 tttgcgattg gataaagcgc attggtttgt agctcaacag gttgtgtaaa gaagtcactt
    61801 tcttttgttg cgtctagttt caataatttg atgacttcgc ctagatcgac ttcttgttcg
    61861 ccttttcgga tcgcgtctgc tgctttttgg ataccagctt tgatatttgg ccagtcattg
    61921 ttgatcaagt ccgcagcagc gttcacagct gtttcgactt ctggcatttt ttcatttact
    61981 gttgtcaatg taccggtgat ttcatttttt agtgctggcc aatcattttg gataaaatca
    62041 gcggccaatc ctaatttttg ctgaatgatc ggtaattcat tgttgtatag gtctgcccct
    62101 tcattgatgc cattgacgat ggtatccata tttccattca gcattgtatt ggcatcatgg
    62161 atttcttgtc caattgcagg catttctttt tgatattttt ctaaaattgc cactgcattc
    62221 gttacagttg ctttcgttga gctgagaagc gaatcaaagt cgatctggtc cgctttgctc
    62281 agtgcgcctt gtgcagtccc aatcgtatcg atgacttggt ctagtagaga agtgacagtg
    62341 gaactgattt gacctgtatt gataccagca atgatgccat tgatattatt aacaacggca
    62401 tcaatttgat ctagcaattc atcaatagcc ttcgtgtctt cattttgaac agctgtgttc
    62461 aaagcagata ggcggttttg cagattgctt agcaagggtt ttaattgatt tagctgatca
    62521 ataattggct gtaatttcgt atttccagca cttgtttgca atttagtcag gaactcgacc
    62581 agtttatcga ttgcattttg ttgttgagat attccgtcaa taaagccgtt caatacattg
    62641 ctgatgtttt tcttttcatc atcagtcagc ttaccatctt caatagctgt tcttacagta
    62701 tctattaacg aacctgtcgt tgttgccact tgtccaattg acttcaatgt aacatctaca
    62761 ctgtttgtga tgcttggtat cgcatctttc agctgagagg cacctgattt tgtttggttt
    62821 gctagatcac ttgcctgatt ttctaaattt ttcacatctg gcaagatatc ttgtacttgt
    62881 tggatgatcg tcaagccttg ttttgcttca tcgattcctt ggttcattgt ctcttggatc
    62941 gaagcaaaat cgccgtcgat ctctgctagt tgcttaccgg cattttggat ctctgggatt
    63001 ttttcttgca aatcaaggat gatttttgct tgatctttca actcaggcat tttgtcattc
    63061 aatgcaacga ctttttcacc catctcatct actttaggga tgtaatccac gaattcattt
    63121 gctttattca gtttttcctt gatttcaggt agttgacttt gtaattcgag gacttgtttt
    63181 gtgtagccgt cgatcgtgtc caaattttca tctgttgata aaatcatatc tttaacttta
    63241 ttgatactta ctaggttcgt atcgatgtcg taacctatct cattgaatac tttcaataaa
    63301 gtcgaactgg ctgttttgat aaattcattt gtgatttgcg attggatcga acttgctcct
    63361 ttatcggtga ttttcggtgc aatcgcattg attttttcat ttaccgtata ttcgattttt
    63421 ggtttcttga tatcaccact agtaaaactc agcagatctt ctgagaaatc ctttggcaag
    63481 taaatacctg cgtaatactt tccggaacgg actccgtctt ctaattgttc ttttgaatca
    63541 acgaattgcc atcctaattg tttattttta tgaagcgatt cgatgacttg ctccccaatg
    63601 gccacttctt taccttgaaa ctctgctggc ttgtctgcac tgtaaacagc aattggcagc
    63661 tctccagtat ttccatatgg atcccataat gctttgatgt taaaccacgc atatagcgaa
    63721 gggatgatca tgatcgcgat gatcagaagt gttgcaatcg ggtttttgaa aatccgtttc
    63781 cagtcaagca aatataatcg aagcgtattt ttgattgact gcatagttct cccccttctt
    63841 cttttttcag ctgaattact ttaccatgta tttcatactt ttttcaatcg gcttttttca
    63901 aaaaagtcga atttttggac atatcacggc aattgtctca ttgtaatctc ttacatattg
    63961 agaaataatt aaggattaca gcctttactt tatcatgaaa taaaagggaa agataatttt
    64021 atgccaaagt ttttatctat tatacgtata cgcaaaaaag ctaaaacatt ttttgtcttt
    64081 gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct tatttttcga
    64141 atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt gcttggatga
    64201 catgcttaag tgaagcagac agattcattg aaataaaaac atgaaaggct cctttttggt
    64261 aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt ggaacaaacg
    64321 attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca tttaatgcaa
    64381 catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata cttcctttcg
    64441 tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt gttttatcat
    64501 aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta ctggatgcac
    64561 tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac ccgaccgcac
    64621 ctgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa tggttgatta
    64681 gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa ctcattttat
    64741 ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa gtcccgccga
    64801 ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa ccaatctctg
    64861 tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt cgtatctctt
    64921 tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct tttatttcta
    64981 cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca aaaacatctt
    65041 gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc ctttttcttt
    65101 aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt ggcgcatgat
    65161 tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc taaaagcgta
    65221 aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg ctaaaaaatc
    65281 aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg ctttacgcca
    65341 ggaattaaaa gcgcaaaaag attacgaagg cttgcgtaaa cttcctttag atgctcgtcc
    65401 agaacgattg aaaaatcgtg atttaatcga tggacgccct cgcggataca tgcgtaaatt
    65461 cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc caggagtaaa
    65521 aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg catggaccac
    65581 tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg gtactagaag
    65641 cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc tgcatggtta
    65701 cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact aattttcaat
    65761 gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc tttcaatctt
    65821 gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc tattcctgtt
    65881 ccagtagctt tgatcccaat gtgagtcgga agcttaggtt tagccatttt tgccgttatg
    65941 gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat aaccaaaact
    66001 gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa ataaatatct
    66061 cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc gctgtcctaa
    66121 tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct gcatagtgat
    66181 ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa attcttgagg
    66241 caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt agttcccttc
    66301 taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac cgatcggtat
    66361 cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga ttccttcacc
    66421 tgctgggagt tttttgactt gaatcaattc agaaaccagc tcaagtgctg gctgcaatgg
    66481 ataaacttcc ggtagtgcgt gcccagaggg attaagtccg tacattgcta ctccataacg
    66541 aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc tgacatgtac
    66601 atatcgagga agttcgggaa gttttttcag cacttctata aagcgttctt tttgcagatt
    66661 ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac cttcccattc
    66721 taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag gtgtacaaaa
    66781 tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg tctctagctt
    66841 acttttctct aaaacagcag ctgctgcttg caaccattcc cgtgtacctg cagttactga
    66901 taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca agataaggat
    66961 gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg aaacacaaaa
    67021 gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat gaccgtatcc
    67081 gttcgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa cttcatttgc
    67141 tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt gccaagcgac
    67201 aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag ctgtttcatt
    67261 tgtatgagta atactgaccc atacattgcc ttcatgaggt gaacgcgtca caacaggctg
    67321 accattttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga tcccagttcc
    67381 ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt ctacttggcg
    67441 atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc gtgcaatgaa
    67501 ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat cgatcccaat
    67561 tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat tctaccaaat
    67621 tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt ctcttaaaaa
    67681 agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc cgcgtttttt
    67741 atcattgtga cgtttaccgc catctttttg gctgttatta tttttgctat aaccgccttt
    67801 tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc cgccaccacg
    67861 tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg gtgtgatctt
    67921 cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg caactaaatc
    67981 ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtatttat ctagaccatt
    68041 ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt tgaatgcttc
    68101 tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt caatcacatg
    68161 caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt ttccggcacg
    68221 gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat catagttgta
    68281 cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg ctacaaggat
    68341 atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt gagacaaatc
    68401 gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca attcgtctac
    68461 acggcgtttt gtacgtccga agacgattgt taattcaggt gtttgaacat caaacaagcg
    68521 agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat cgatcaagtc
    68581 agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga atttaacgcc
    68641 aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc tttgatcagg
    68701 aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca tttcatctgc
    68761 ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt taatgtggtc
    68821 aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc cacggatctg
    68881 acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt cgcgccctaa
    68941 acgatataat tcttcttgtg tttggatagc taattcacgt gttggtgcga taactaaacc
    69001 ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa atgcagctgt
    69061 tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg ctagtggaat
    69121 cgtttctgct tggataggcg ttgcttcttc aaaaccagcg cgttcgactg atttcaacaa
    69181 ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg gttgttaact
    69241 gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat tgtttctttt
    69301 tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac cgaaaaatca
    69361 ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt atcattatta
    69421 tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc tcttcatctt
    69481 tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag atttttattt
    69541 catttgctga agttgttcta ccacttcaaa taagcccatg ctgttgctgc cttttagcat
    69601 aatactatct gttgcttcaa gattttgttt gatagcatca atcaatttat tcttttcttt
    69661 ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt ttagtgcatg
    69721 catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat gttcactcat
    69781 ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta acacagcgat
    69841 tctgcgtcct tttgttggca gatttttaaa agtatctaat accagtccca tggctgttgg
    69901 atttgcattg tagacatcgc tcaaaatagc tgcaccattt cctgccgtta gccattcggt
    69961 tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta tggacagtcc
    70021 aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac ttccaggcag
    70081 cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc cttttgctgt
    70141 cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt gtactaatgt
    70201 tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc cgtctgatac
    70261 aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc caagattttc
    70321 tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca taagtgaaag
    70381 ttccgtgatt tcattggcgt gatccatgcc catttccaaa acgatctttt ctgtatcttg
    70441 gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc cttgcgtctt
    70501 atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg tggttttacc
    70561 attgcttcct gtaattgcaa cgacttctgc gcctactttt tccaagtaat aggttgccaa
    70621 ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatttt ttggagcttg
    70681 ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt gctggataaa
    70741 ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg ttatcttccg
    70801 gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc ccacggcttg
    70861 tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt gtgaaaaaag
    70921 cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt ctctattcgt
    70981 atttagaagc tagaatgaac gcggttacgc aatcactcca gcctcttctt tatttctctt
    71041 tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc taactggatc
    71101 agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag tgggtacata
    71161 ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc atttttattt
    71221 gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta agctaacttc
    71281 gcgtactctt gcgctttttg ataaacttct tctggcactt cggctggaat ctgcatttcg
    71341 attttattat tgatatattt tgcttcataa tcatagaatg ctacgtcttt tacgacttca
    71401 ccaggcaaag tcgtccgaac gtcttcattt cctaatacag caacttcgat ttcgcgcgct
    71461 tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc taaagcattt
    71521 tgcagctctt ctcggttttc tgcttttgta atgccgacac tagaacccat attcgccggt
    71581 ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt tttaggattt
    71641 tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc agcagcttgt
    71701 aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac gcctgcgccg
    71761 acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc cccgtttggc
    71821 ccatgtaaaa ctggaaaaac gatggctcct tcttctttga tttcgcccgg attgatcact
    71881 tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg caagacatct
    71941 ttgctggcag gtttttctgt tagtaatgga cctttgaccc attgtccttc ttttgtaata
    72001 aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga aaatgcggaa
    72061 agaatggaca cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat cttcaaaaga
    72121 ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc acaaaacaaa
    72181 aataaacgaa taacttattt ataaaaaaac gagccagctt ctaaaagaac gagcgtcatt
    72241 tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga taaagaaaag
    72301 gagggatgat gatggcgcaa acaaatccac gcagccgctt ggcagaaatc attcgagtgt
    72361 tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa caggtaaaaa
    72421 aagcctttga agaactaggc cccaccttca tcaagatagg acagatgctt tctgttcgag
    72481 aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat agtgtaccta
    72541 gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt tcattatcag
    72601 atatattcga tgatttttca aaatcgcctt ttgcctctgc ttctatgggg caggctcatc
    72661 gtgccaagct gaagaacggt gacgcggtag tcgttaagat ccaacaccct aacatcgccg
    72721 aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc aaatacattc
    72781 ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt tccttgatca
    72841 atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa aagaacaatg
    72901 gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa aaagtgatcg
    72961 tgatggaaga aatgtctggt aaaaacttga atcatttaat gaacgccgaa aacaaaactg
    73021 aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca aagcttttag
    73081 ttgagaactt tatgaaacag gtgtttgatg atggtttttt ccatgctgat cctcatccag
    73141 ggaatctgct attccatgta ctaacaaaag aagaacagac acaagctagc cgaaaaactg
    73201 aaacagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact tctgcagaag
    73261 accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg catctttctg
    73321 caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa gatgcttatc
    73381 gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat gaaagtcggt
    73441 tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt gatgaaatca
    73501 acttgcaaga agtattcaca catgtcgtga cgatttgtca tcaaaataat ctgcagttcg
    73561 atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg atccgcgtcc
    73621 tcgatcctga agtttccctc atggaagtcg cttcgccttt tgctcaacat tattttctca
    73681 ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg gaagggatga
    73741 aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg acttcgggac
    73801 aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg atcgaaagta
    73861 tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg tcctccctgc
    73921 ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg atatttacgt
    73981 atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc caaatgtata
    74041 agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag aaataagaag
    74101 gggttttaca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt ttcgaagagg
    74161 ctacttctga aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc cagtaataag
    74221 aacaatcaaa aagggaacat ggaacacatt tatcctgttt ccctgttccc tttttgatgt
    74281 attcgagcat aaataaaata ccttcgttac ctactatctt ctgtacttgg taaaccttag
    74341 tcaattgcct tttttaacgc ttgacgaaaa gacatatgat ctttctccgt aaagggtttt
    74401 ggaccctttg tccttatgcc agcttttctt aactttccac tatgataacg ttgttctaga
    74461 agcgtgccga tattcgcttc gtcaaattct tttccagagt gatggaacac tcgcacaccc
    74521 ttcggcaata caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc attcttctta
    74581 accataccga cgattttata atccgcacta tcagctccgc ggtctacgta gacgatttgg
    74641 acatgattag cgtattcttt ggtcgtataa tggtccacac tggtcacgat aaccacgtca
    74701 agaccatagg attctcctaa ttttatgaca tcttctttta ctggagaacc atctccatca
    74761 atgaatagcc tcattctttt tcgccttctt ccataaatga taaacagtta ctaatccgct
    74821 cagtaaaaga ccaacattga tccattttgc caagataagc agttgtacac tttctatcgg
    74881 cactgattca tggttcagcg tgatcaataa tagatggatt ggctctccca tttcttttgc
    74941 taaaacaaac aaaagcgtca tcaataaaaa ctggtggatc acactccctt tattttgagt
    75001 agtatacttg acaacgatcc ggcgatccat tggaatagct gtatcagttt gataatattc
    75061 cttatttagc cagctgcctg ttactacgtg ccagaaacta agcagaaaga cacctatcgc
    75121 aaaaccattt ggaatcaaga ggaccaataa agaagaactc agtccaagca attgcatagc
    75181 caatggagac catgttaaac gtttcaaaat ccatggtccg actatttttg ctgcaatcaa
    75241 gcctgctgca taaggcaaat acataaaaat cgaaagtttt tctaatccgt acagtatgcc
    75301 aacataaaaa ctgctaaata aaaaaaggaa attccctagc attccattta gaaaagtaaa
    75361 cagatttagc cagcttggca acttccattc cttcttctgg tgtcctaaaa caaacacgaa
    75421 caagatgaac agcagcaaga aaccataaat agcaagatca aaaagccttt catcgaataa
    75481 caatcgggca tttctcaaaa gaaacaataa gccaaagaat atcaaaaata aaacaagctc
    75541 tcgcgtagct accagatttc ttttgatgtc tttgaaatcc agttcgtaac gcggataatg
    75601 actaaccgta tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc ttgctgcaat
    75661 ctgagtcggt gcaggcaact caatcgcttg atacaggaga aatagccaga tagctgcaaa
    75721 taagtacttt ttcccatcca tattgataaa accttgcgtc ttttcgtgaa aattgaccgt
    75781 ggtattagca ggtacaagcc atgctgcact cagccctagc aaaacacttg aaaaataaaa
    75841 taaaggaaaa taaaatacgc ctaatattcc aagcaatgaa ccagcaccac caatcagcag
    75901 agcgatcatc agcaacgtgt aactatctag accaaatcgt agactgttga ctaaaaatag
    75961 tccagtcatc cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc aattatgcga
    76021 gtctgttagt cccaaaaata aaagataagg aataaaaata accgtgttac ttaagataaa
    76081 gggcgttgtt tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca tgacaatggc
    76141 tccttaaaca gtcttataac taatctgctc ttcgatttta gcattaaagg ggaaataaac
    76201 caattttaca gaatatattc ttgtaaaaaa aaatacagaa agaagcataa gcttctttct
    76261 gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa agtcttgccc
    76321 gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg cctccaaaat
    76381 caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt tactttaaca
    76441 cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt gaaatccttg
    76501 accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat aaacctcgat
    76561 tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg tttgcgcagc
    76621 aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta ctttatttag
    76681 caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag tgtaaccgac
    76741 agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt ctttttgaat
    76801 ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac caatgatcgg
    76861 cacaaaaact tccggccata cttcttgtat gactgccatc agctcatcca gttcattttc
    76921 tgttactttt attttttcca tacacccacg tcctttttta cattataaat gaccttgcgc
    76981 ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccacg gtttcaagaa
    77041 aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta ctagacagat
    77101 caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt taatatcttt
    77161 cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata aaataagctg
    77221 gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc caatcactag
    77281 gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc ccgctactga
    77341 ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca gaacaaatat
    77401 catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc tcgatcgaaa
    77461 aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac caataatggg
    77521 catgatctga agacattttt gtaaaactga tcctttttta gacatctata ttccccacat
    77581 cttttttcat ttattaaaac tgcctcctt
//
LOCUS       DAYYBG010000004        61155 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000004 DAYYBG010000000
VERSION     DAYYBG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.004"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACHAUN_000249"
     CDS             complement(38..1189)
                     /locus_tag="ACHAUN_000249"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8458985.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACHAUN_000250"
     CDS             complement(1202..1639)
                     /locus_tag="ACHAUN_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8458986.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACHAUN_000251"
     CDS             complement(1654..3420)
                     /locus_tag="ACHAUN_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8458987.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACHAUN_000252"
     CDS             3684..4361
                     /locus_tag="ACHAUN_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8458988.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACHAUN_000253"
     CDS             4364..5491
                     /locus_tag="ACHAUN_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8458989.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACHAUN_000254"
     CDS             complement(5691..6131)
                     /locus_tag="ACHAUN_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8458990.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACHAUN_000255"
     CDS             complement(6142..8175)
                     /locus_tag="ACHAUN_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8458991.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACHAUN_000256"
     CDS             complement(8177..9880)
                     /locus_tag="ACHAUN_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8458992.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHAUN_000257"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHAUN_000257"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8458993.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACHAUN_000258"
     CDS             complement(10963..11988)
                     /locus_tag="ACHAUN_000258"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8458994.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACHAUN_000259"
     CDS             complement(12197..13066)
                     /locus_tag="ACHAUN_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8458995.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACHAUN_000260"
     CDS             complement(13525..15417)
                     /locus_tag="ACHAUN_000260"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8458996.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACHAUN_000261"
     CDS             complement(15414..17141)
                     /locus_tag="ACHAUN_000261"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8458997.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACHAUN_000262"
     CDS             complement(17157..17807)
                     /locus_tag="ACHAUN_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8458998.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACHAUN_000263"
     CDS             18011..18307
                     /locus_tag="ACHAUN_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8458999.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACHAUN_000264"
     CDS             18479..19099
                     /locus_tag="ACHAUN_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8459000.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACHAUN_000265"
     CDS             complement(19191..20357)
                     /locus_tag="ACHAUN_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8459001.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACHAUN_000266"
     CDS             complement(20369..20722)
                     /locus_tag="ACHAUN_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8459002.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACHAUN_000267"
     CDS             20850..21872
                     /locus_tag="ACHAUN_000267"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8459003.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACHAUN_000268"
     CDS             21891..22052
                     /locus_tag="ACHAUN_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459004.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACHAUN_000269"
     CDS             complement(22311..22745)
                     /locus_tag="ACHAUN_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459005.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACHAUN_000270"
     CDS             complement(22855..24714)
                     /locus_tag="ACHAUN_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8459006.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACHAUN_000271"
     CDS             24715..24855
                     /locus_tag="ACHAUN_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8459007.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACHAUN_000272"
     CDS             complement(24832..25992)
                     /locus_tag="ACHAUN_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8459008.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHAUN_000273"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHAUN_000273"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8459009.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACHAUN_000274"
     CDS             complement(27739..28437)
                     /locus_tag="ACHAUN_000274"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8459010.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACHAUN_000275"
     CDS             complement(28394..30055)
                     /locus_tag="ACHAUN_000275"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8459011.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACHAUN_000276"
     CDS             30273..31673
                     /locus_tag="ACHAUN_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8459012.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACHAUN_000277"
     CDS             complement(31925..33004)
                     /locus_tag="ACHAUN_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8459013.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACHAUN_000278"
     CDS             complement(33090..34049)
                     /locus_tag="ACHAUN_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8459014.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACHAUN_000279"
     CDS             complement(34061..35572)
                     /locus_tag="ACHAUN_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8459015.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACHAUN_000280"
     CDS             complement(35594..36157)
                     /locus_tag="ACHAUN_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8459016.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACHAUN_000281"
     CDS             complement(36163..37026)
                     /locus_tag="ACHAUN_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459017.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACHAUN_000282"
     CDS             complement(37040..37936)
                     /locus_tag="ACHAUN_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459018.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACHAUN_000283"
     CDS             complement(38111..39400)
                     /locus_tag="ACHAUN_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8459019.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACHAUN_000284"
     CDS             complement(39372..40889)
                     /locus_tag="ACHAUN_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8459020.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ACHAUN_000285"
     CDS             41087..41977
                     /locus_tag="ACHAUN_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8459021.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHAUN_000286"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHAUN_000286"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8459022.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ACHAUN_000287"
     CDS             complement(42886..44358)
                     /locus_tag="ACHAUN_000287"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8459023.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="ACHAUN_000288"
     CDS             complement(44995..45357)
                     /locus_tag="ACHAUN_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8459024.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="ACHAUN_000289"
     CDS             45580..46332
                     /locus_tag="ACHAUN_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8459025.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="ACHAUN_000290"
     CDS             complement(46472..47395)
                     /locus_tag="ACHAUN_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8459026.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="ACHAUN_000291"
     CDS             complement(47410..47682)
                     /locus_tag="ACHAUN_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459027.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(48152..49777)
                     /gene="groL"
                     /locus_tag="ACHAUN_000292"
     CDS             complement(48152..49777)
                     /gene="groL"
                     /locus_tag="ACHAUN_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8459028.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(49828..50112)
                     /gene="groES"
                     /locus_tag="ACHAUN_000293"
     CDS             complement(49828..50112)
                     /gene="groES"
                     /locus_tag="ACHAUN_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8459029.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            50337..50999
                     /locus_tag="ACHAUN_000294"
     CDS             50337..50999
                     /locus_tag="ACHAUN_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8459030.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(51075..52367)
                     /locus_tag="ACHAUN_000295"
     CDS             complement(51075..52367)
                     /locus_tag="ACHAUN_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8459031.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            52537..53166
                     /locus_tag="ACHAUN_000296"
     CDS             52537..53166
                     /locus_tag="ACHAUN_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8459032.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(53269..54078)
                     /locus_tag="ACHAUN_000297"
     CDS             complement(53269..54078)
                     /locus_tag="ACHAUN_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8459033.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(54133..55002)
                     /locus_tag="ACHAUN_000298"
     CDS             complement(54133..55002)
                     /locus_tag="ACHAUN_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8459034.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(55003..56319)
                     /gene="yycH"
                     /locus_tag="ACHAUN_000299"
     CDS             complement(55003..56319)
                     /gene="yycH"
                     /locus_tag="ACHAUN_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8459035.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(56316..58151)
                     /gene="walK"
                     /locus_tag="ACHAUN_000300"
     CDS             complement(56316..58151)
                     /gene="walK"
                     /locus_tag="ACHAUN_000300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8459036.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(58156..58860)
                     /gene="yycF"
                     /locus_tag="ACHAUN_000301"
     CDS             complement(58156..58860)
                     /gene="yycF"
                     /locus_tag="ACHAUN_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8459037.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(59050..59910)
                     /locus_tag="ACHAUN_000302"
     CDS             complement(59050..59910)
                     /locus_tag="ACHAUN_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8459038.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(59897..60466)
                     /locus_tag="ACHAUN_000303"
     CDS             complement(59897..60466)
                     /locus_tag="ACHAUN_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8459039.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            60847..60919
                     /locus_tag="ACHAUN_000304"
     tRNA            60847..60919
                     /locus_tag="ACHAUN_000304"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60880..60882,aa:Lys,seq:ctt)
BASE COUNT        17665 a        12800 c        10546 g        20144 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgcc ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgt
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatggatatt tacttttaat agataaaata
    47881 ttataactat taaaaataac tattttcatt ataattacac acaaaaaaga gcgtgagaca
    47941 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacagcgga agcagaagca
    48001 actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaatttcc gtgaatttct
    48061 tcttatttct tggagttaaa cgcttctgtt ccagcctctt tttatctgtc tttcactaat
    48121 gatcagttgt agataagcaa gttgcttttt cttacatcat accgcccatc attgatggat
    48181 ccattgcagg agctgctggc gctgctggtt ctggtttatc tgcaacgact gcttctgtcg
    48241 ttaataacaa ggcagaaacg gaagcggcat tttgcaaggc agaacgtgtt actttagttg
    48301 ggtctacgat acctgcttca accatgttta cccattctcc agtagcagca ttgaaaccag
    48361 tgcctagttc cacgttcttc aatttatcaa caatcactga tccttcataa ccagcgtttt
    48421 cagcgatttg acgaattggt tcttctaaag cacgaacaac gatcttgata cctgttgcca
    48481 cgtctccttc tgcttctaca gcagaaactt tgctgataac gttcactaga gctgtaccac
    48541 cacctgagac cattccttct tctactgcgg cacgtgtagc attcaatgca tcttcaatgc
    48601 gtaattttaa ttctttcaat tctgtttcag tagctgcacc aactttaaca acagctacac
    48661 cacctgctaa tttagctaag cgttcttgta atttttcgcg gtcaaaatca gaagttgttt
    48721 cagcaatttg gtttttgatc aattgcacac gagcttcgat tgcttctttt tctccagaac
    48781 cttctacgat cgttgtattg tctttatcta caacaacttt agaagcattt cctaagtttt
    48841 cgattgttgc atctttcaat tctaaaccta gatcgtcagt aattactgta ccacctgtca
    48901 aaatagcgat atcttcaagc attgctttac gacgatcgcc gaaaccagga gcttttacag
    48961 ctactacgtt gaatgttcca cggattttgt tcaatacaag agttggcaat gcttcaccat
    49021 ctacatcgtc agcaataatc aacaatggac gtgattgttg caagatttgt tctaacaatg
    49081 gcagaatatc ttggatgtta gagatttttt tgtctgtgat caagatatat gggttttcta
    49141 gaacagcttc cattttgtcg ttatctgtta ccatatattg tgataagtaa ccacgatcaa
    49201 actgcatacc ttctacaaca tctagttctg tttcgatccc ttttgattct tcgattgtga
    49261 tgacaccgtc gtttccaact ttttccattg catcagcaat caaatgtcca actttatcag
    49321 aacccgaaga aacagctgct acttgtgcaa tggcttcttt tgaatcaaca acagttgaga
    49381 tattatgcaa ttcttcgact gctgcttttg ttgccaattc gatcccgcga cgaatgccta
    49441 gaggattagc acctgcagtt acgtttttta aaccttcgcg aacgattgct tgtgtcagaa
    49501 cagttgctgt cgttgtaccg tctcctgcaa tatcatttgt ttttgatgct acttctgata
    49561 caagttttgc acccatgttt tcaaaatgat cttctaattc gatttctttt gcgatcgtca
    49621 caccatcatt tgtgatcaat ggagagccgt aagatttttc tagtacgaca tttcggcctt
    49681 taggtcctaa tgttactttc actgtatctg ctaatttatc taccccgcgt aacatagctg
    49741 cgcgtgcgtc ttctgcaaat ttcaattctt ttgccatgat tgattcacct cattgattat
    49801 ttttgattat ttttttatct tccaaattta ctcaacaatc gccatgatat cttttcctgc
    49861 gacgatcaga tattcttttc cttcgtattt tacttctgta ccggcgtatt tttcgaacat
    49921 aacttggtcg cctgctttta cagcggcagg aactttttca ccgttttcta acaaacggcc
    49981 ttcaccgact gcaacaactg tcccagtttg gggtttctct ttagcagcag aagctaaaac
    50041 gattcctccg acagtttttt cttcttcttt tgcgacttcg ataatgacgc gatcacctaa
    50101 tggttttaac acgataaatc cctccgataa ctcttttttt aatctgttag cactcttaac
    50161 cttcgagtgc taacctacat ttttaataat actcattctc cctctttttt gcaagctttt
    50221 aaatcaaaga aaagaaattt tattctgaca gctgcttatt gaaattatga tataattgaa
    50281 atcaatcgat ataagcttgc cttgcatgct tgttatccct taaaaggagg aattacatgt
    50341 ctttaaaaaa atatagcttc ctcagcattt tttgctatgg tctaattttt atctcaccct
    50401 tgatcctagc ttcgatagga cttgtaaaat ctacgtctga attgatcaca gcaactgcag
    50461 tcatgtatat actaggcgcc gttgtcctag ctatttttta ttttaagcaa cgtgaaccct
    50521 tagcgatcga atcagcggta aaaaaagctt ctgctccaaa gattgttatt tatggattgg
    50581 ttggaatctt tgtcgcattg attctgcaaa gcatcgctgt catgttagaa tctgtattgt
    50641 ttggcaaagc aaccccttcc gagaatacac aaaatatcat ccagatgatc atggaagcac
    50701 ccgcttttat tttggcgaca actgttgctg gacccatcat ggaagagttc gtattccgcc
    50761 gcagtatact ggggatcatc agtcgatatt caaatttttg ggtcggagca gtgatcagct
    50821 ctttgctgtt tgcgtttgct cataatgatg gtcatctgct aatctacttt ttcctaggtt
    50881 tcttcttcag cctagagtac aaagcaactg gtcggatctg gacatcgatg atcacacacg
    50941 taggaatgaa cacattagtt gttcttgttc aattagctgt ccaaaaaggg ctagtataaa
    51001 catgactata aatactcctg tcatcctaga ttggtgattt tcccaatcta aagcgacagg
    51061 agtatttttg ttttttagtc gttcatcttt tctgctgctc catcattttt tgtctgtctt
    51121 tcctttttgc agtttgcaga atctattttt ttcaaaaatg ggaaataaat gaaaaaacta
    51181 attgacaaca cgataccttg taaaatcgct gcaggcactc cgcctactaa gaaacccgag
    51241 aggatcggcg gtgttgtcca agggacttgg actgctgtaa ataaaggaac gaatccaaaa
    51301 tataaagcgg aataggtaat caatcctgaa acgaccggtg cgaaaataaa aggaacagcc
    51361 atcatcgggt tcatcacgat cggtgtggaa aaaataattg attcattgat gttgaaaaag
    51421 gctggtaaaa gagcaagatt tcctaattct ctgtatttct tcgatttcgc aaaaaccgtc
    51481 ataaaaaata cagcccctaa cgtcaatcca gcacccgtaa ccgtcatgaa ctgatcgaga
    51541 aattgctgag taacgatatg tccgccattt gccaccgtca actcttgtcc tgaggctaag
    51601 atttctgcat ttgctaatga attcgcttgc aagacgggac taaccaaccc attgaccatc
    51661 gcagatccat gaagaccaaa taaccataaa aacggaacaa aaaaacctaa taaaagcaca
    51721 ccaaacaacg tgtctatgaa cgattgaatg ggaagctgga catatgtcca tactccttga
    51781 acaacagtca ttctgaacaa atgttgaaaa accgctgaac cgataagcgt aaaagtgact
    51841 ataacaatga ttggaatcaa tgaagtaaaa gactgccaaa cattttcagg tatcttctct
    51901 ctttgcttcc accctttcgc aagtcttact aaacgtgcat agccccatcc gatgatgagc
    51961 ccagttgtaa ctgctcctag tatcccttca gcacctgtcc agacagtatc tatctgagta
    52021 tttagtggct gaataagcag tagataagca gacaaggctg ttatgccgac tggtaagcga
    52081 tcgatgtctt cttcttttgc ataagcgtat gcaatggcaa aaacgatcca aaaggctatc
    52141 acatcaaatg tagcctggta tacttgtgta aaatacatag aaaatcctgt tttgtctatc
    52201 cactgagcga tgtaatccac aggaaaatga gcaaataaga aaaacagcga gccaacaata
    52261 attattggga tcgtgtaagt cattccatta cgcaaagcag ttaagggtct aagattcaca
    52321 acagatgtta tcattggtaa aaaggcacga tctaaccact ctttcatttc ttatctcctt
    52381 caacttctat ttttatcaat cagttttctt aatttaacta tacccaatga tcacgaacca
    52441 tgaaaggaat gcgcactgag cttttcaaaa atcggattct ttgacgaata ttcagcaact
    52501 gcttacaatg gacaggaata aaaaaggagg ttttttatga aaattgcact cattggagct
    52561 actggccatg caggttcatt tattttagac gaagcattgt ccagagattt agacgtgacg
    52621 gcaatcgtgc gtcatccaga aaaattgcca tcagatgttc cgtttataca aaaggattta
    52681 tttgagttga caactactga tcttgcatct tttgatgttg tcgttgatgc tttcaacgct
    52741 cccaaaggaa aagaagaaat gcatcaaact tctttgtccc atttgattga gatgcttgaa
    52801 ggaaccgata cacgcttaat tgtcgttggg ggtgcctctt cgttattctt agatacagaa
    52861 aagacgactc gtatgattga ccaagtacct aaaaatgcac ccttctatcc aacagctttc
    52921 aatatgtacg aaggattgtt aaaattgaaa caatctaaaa atctacattg gacttatatc
    52981 agtccagctt ctttatttga tccacatggc aaacgtacag gtacttattt gttaagtgat
    53041 gattatttac aaagaaacgc tgctagcgac agtacgatca gcatggcaga ttacgcgatt
    53101 gcattagtag atgagatact ggatgagaaa catgaaaagc agcacatcag tgtagtcagt
    53161 cgataatacg gttatttttt taatatgcta aaaaaataaa aatagaagat aaaaccaacc
    53221 ctgattgatt ttatcttctg ctttttatat ttttattgat tgaactgttt aaatcgaaaa
    53281 tagctcagtt gcttcatctg gatctgtatc atagatttca aaatcatgtc caacgccaaa
    53341 atctttttct ttcaaaacat tttccattgt taaatgagca agttctttca tattgttttc
    53401 cttgctaaga tggccaagat agattcgttt ggtccgatta ccgatcacat cagtcatgac
    53461 aagtgcaccg tcttcgttgg aaagatgtcc tctatctcct aaaatacgct gttttaaatt
    53521 ccacggataa ggtcccatgc gtaacatctc aagatcatga ttactttcca tcaaataacc
    53581 atcggcattt tcaatgacac ctcgcatata atcactgcaa tatccggtgt ccgtcaacat
    53641 gacgaatgac cgattttctt tatggaaacg ataaaattgc ggagccgctg cgtcatggga
    53701 gacgccgaaa ctctcaatat cgatatcgcc gaatgttctg actttcccca tctcaaatag
    53761 atgtttttgt tccactggta catttccaat cagtggttcc atcgcttccc aagtctgctg
    53821 attggcaaaa acatccaaat gatacttacg agccaagaca cccacgccat gtatatgatc
    53881 tcgatgttca tgcgtcacaa gtattccatc taattggtcc ggtgtccgat caatttgagc
    53941 catcaaggaa gtgatttttt taccgctaag tcctgcatcg accaaaagcc gttttttttc
    54001 actttcgata taaagtgaat tacccgaact tccacttgct aggatactga ttttaaaagc
    54061 tggtttttct tcagccattt gactcctact tcccttctta ctttgtaaac ttttttcatt
    54121 atactcgctt ttttattgat cttccacttt tggtacagta ttgtttgtca cgatcgtatt
    54181 gctgaaagcg ttgacgctct caatttggac cactttatcg cttgtttcga tcgctacatg
    54241 ccaaacagga acatacacat ttttttcccg gactttcaga atacaggaat aagctagctg
    54301 acgccataaa atttttgatc ctcttggtat tttgttattg atgtacaatg tattgattgc
    54361 ttcctcttcc gtatacagtt ccgtcttttc gcgcagctgc tcgatatcag ataaatgcgt
    54421 ctgcatgtat ttcgtgatgc tataatcatt atcgtttttt tctaaagaaa gaacgatttt
    54481 tgctgtatca tcattgaatg ggatgccttc atactcttgg cttgcttgga tttctagtgg
    54541 ttcttcttct aaatgagaaa agtttttcgt gtacacataa tcattaccga ataaaatatt
    54601 gtctttgtta gataaaaaat cttccaagga actactaatt ttatctggat cgatcacgtt
    54661 cttttcagca tctgaactgg ataaagattt cgtcaaaaca tcaccgtcta aaaacacacc
    54721 acttgccagc aagccgcttt taccagcctt gcgataattc gctaatgaag tgctaagatt
    54781 ggtctgctcc gcgctaagat aatagccttg cttgttctca gacgaaatct tccccgtgta
    54841 agtgatatcg tcgctagcca gacgttgttg gattgattca gtctgattag agcgaatgac
    54901 attgttttct tggtataccg cgtcatgata actgctgaat aaaaacacat tcaaccctaa
    54961 aaaagcgacg aaaaatatcc attcgatttt tttgaaatcc aattaattcg cctccaatcc
    55021 aggtaatttt tccagaaggt cgttagctga ataccagtta tctttatatt tcacatacca
    55081 ttcaggtaag agatcaacga cacgattcgt ttctttgaca ttttgccatg tatagccaac
    55141 gatcactgaa ccaatcttgt tgctatctgc tcccttagct tttaactgct ggatgatttc
    55201 attcgtactt gcaagttcta cttcttcgtc tgaaggtatc ggcacttgaa tcgtattcaa
    55261 actggtttgg atattcactt gcacagaacc attcgtttca ccgccaactt ccacaccaat
    55321 ctgtcctctg ctgtctgtcc caaaaaccgg gaagccttcc acgaatatcc gataatcgat
    55381 cgtattgtca ctgcggtcaa aatatctgag gttccctaaa gaactgccta atttgctaat
    55441 ataggggaaa ctttcagaaa atacactagc attttctttt ttctctggca gctctccacg
    55501 aaaatcaact acttgcgttt ccgaatggat agacattgtt tcattcccac cgtaaaaaat
    55561 caattcattg ctttcatcat tatttttcac gctatctggt ttctggaaaa aagcatttct
    55621 aaaaagtgta tagggttgct gtgacaggat ataactatac ttcttcagtt tgatcgaatg
    55681 tttcgtagca tattgcgtgt cgatcgtcga tggttctgtt gtcatttctg tccattttgt
    55741 gtcttttgct gttaattctt tattgatatc tgtccaattc aacgaaatat ttgcttcata
    55801 tacgagatgg ttcgaataat tcaaaaatcg gattttttta tttccttgat cgaattgtat
    55861 tctagtaaaa tacatatttt cgctttcttt tgccaagttc gacacatcta aatctaaatc
    55921 aaatgcttcc ttgtattctc ttagcaaaaa aggcgcatta taggccaact caaaaccaga
    55981 gttcagattt ttcacctgtt caaactgtga tttgtctcct gaaactactt cagaaagctt
    56041 tccaaatctt gctttatcga tgatcaattg caatcggtac acgagatttt cactgttggt
    56101 ttcttggatc attcccgaat ggtgccatgt aacatgaagc ggaagaaaga cttcggaagt
    56161 ttttcgattg ctttgagact cgatcatttg agtgtttgtc tcatctacgt tgattgaact
    56221 ttttcctgct gggctcagcc agattgcata agagaagtat atgcttaacg caaccatcaa
    56281 aatgagtcct atacgaacga ctttttcaga gattctcatt cccaccattc ctcctcataa
    56341 ggttcatatg gcagcgagat ataaaaagtc gacccttttc cttccttgct ctctgcccaa
    56401 atagcgcctc gatgcgcctt aatcacttct ctagaaatag ctaatccaag tcctgtccca
    56461 ccttgtgctc ttgcacgtgc tttatctact cgatagaacc gctcgaacac tcggttcaga
    56521 tcttttttag gaatccccaa tccttgatcg gtgatactta aaatcacgtt attatgcgtt
    56581 tctaataagc gacaagtaat cgttccccca tctggtgaat acttgatcgc gttattcatg
    56641 atattatcga ctacttggat gattttatct gtatctactt cgacccaaag atctctttgc
    56701 gtaaattcac gacggatctt ataatttttt tcctgatttc ccaccatcat atcaaaccgg
    56761 tcaagaacaa aattcactaa ctcgttgaaa ttgacatatt ctaattgaag ctgggtatta
    56821 cctgtatcca tgcgagatag atttaagagg tcattgatca ttcgaatcat tcggtctgtt
    56881 tcatctagcg ttaccttcaa aaaatttggt gctacttcct cgtctttcca cgcaccttcg
    56941 ctcaatgctt cgatataact acgcatactt gtcaacggtg tacgaagttc atgggaaaca
    57001 ttagagacga attcacgacg ttcccgttct gttttttctt gttctgtcac atcatgcaag
    57061 acagctacta atccgctgat aaaacctgat tcgcgacgga tcatagaaaa gtctactcgc
    57121 aagatcatct gatcactttc caatgtactg gttgaacggt caagtaccat ttcgtttgga
    57181 tcttctaata atttccgcaa cgtatattct tcttcgatat ctaaaagtgt caaaatcgat
    57241 tgtccgattg cttcctcgtt tttgatatct agcaaggaaa cagccatatc attgatcgta
    57301 atgactttac ccctgcgatc tgtcgcaatg acgccgtctg tcatatggga tagcacgcta
    57361 tccaaacgat tgcgttcagc ttccattgta tcttgagctt cttcaatacg ttcagctaac
    57421 tgattgaagg tatcagccag ctgtccaagt tcatcttgtc catgcacttt gactttccga
    57481 ctgtagtctc ctctagcgat tcgaatggct tgttctctca tttcaccgat cggctgtgta
    57541 atcgaacgag caactaaaac agatacgatc attgaaataa atgccgcaat tagtgaggca
    57601 gtaaagaaaa tgactgctgt gttattgatt tccgaatatt tttgttcgat attacttttg
    57661 acgtaaagag ccccgataac ggcatcgccg gtcggggaca ggatcggttg aacattgata
    57721 ttcacccgtt tatcgtcgtt atctaaagca acataacgtt tagtcgtgaa atcattgata
    57781 tcaacataat catttttctt tcccacagcg ctttgttcat tgacatcggt tgttcctcgg
    57841 atgatccctt tttcgtcaac gacccgtatt tcgatgatat cgctagtcgc actgttttca
    57901 ataaatcttt tgatattcgc atttacctct tcatcatctg cattatcttt tcgattcaat
    57961 tccgggctga ttgttgttgc taatgattcc accgtttgat tcatatcttt aataaatgta
    58021 ttgatcgtgg aacgttctaa accacgtata aagtaggcac caataatttc gatcgagatc
    58081 aataaaatca gaataaaggt aatagcgatc ttaaaattga ctgatcgaaa aaagcggact
    58141 tttcttttca tccaattact cctgttctgg gttacgcaaa tagtaaccga caccacgacg
    58201 tgtcactaaa taatttggat ggcttggatt atcttcgatc ttttcacgca aacgacgtac
    58261 agttacatcc actgttcgta catctccaaa ataatcataa ccccaaaccg tttgtaataa
    58321 atgttctctt gtcatcactt gtccaaggtg cttcgccaag taaaagagta gttcgaattc
    58381 acgatgtgtt aactcgattg tttctcctcg tttcgtcacc atgtaagctt ctggatggat
    58441 cgtcaaatca ccaatcgtca attccgcagg tgctgcctct tctggttcct tcgcagccgt
    58501 ggcaccgcga cgaagatttg ctttgacacg tgcaactaat tctcggttgg aaaatggctt
    58561 ggtcacgtaa tcatcggcgc ctaactctaa gcctaggact ttatcaattt cagaatcttt
    58621 ggctgttacc ataatgatcg gcatatcata agttttgcgt acctcacgag caacttctaa
    58681 gccgtccatt ttaggtagca tcaaatcaag taaaatcaaa tcaggttcta cctcagcaac
    58741 tttttccaag gcttcttccc catcatatgc agtataaacg tcatagcctt ctttggctaa
    58801 attgaatttg acgatatccg aaatcggctt ctcatcatcg acaactaaaa cttttttcat
    58861 aaagagccac ctcttatata ttattgtcat accttctcat tataccgtat ttctgctttt
    58921 gtttcatctt tgcctataaa acaaaagaga aaattaaaat gaaagtgcaa aatactttat
    58981 tattttttgt tctaacttca ataaaaaagg aaaacactga ttttcacctc agtgtttcct
    59041 ttttttgatt tatccttcta aaacttgtac accgacaccc aataatcccg gaccagtgtg
    59101 gactactaat gccggagaaa tctggccaaa gtagatttgg tttgcttgtg ggaactctct
    59161 tttaagtctc gcttccattt caattgcttc ttctttcgca tccccatggg caacagctaa
    59221 gttgaagacc tttgcattac cgattttttc tttgacgtaa gcaaccgttt tatccaaact
    59281 ttttttacgt cccctagctt ttcctactgt gtaataaatc ccttcttcat tacatgagat
    59341 gattgggtta agctttaaag cggttcctac aatagaagca actaaaccga tccgtccacc
    59401 tttttgaaga tattccaatg tcgcaacatt aaagaaaact tttgttcttt cgactaactc
    59461 attcagatta ctgatcagtt gatcccattc tactccttct tccagccatt ttgctgcttg
    59521 gatcgcagaa aaacctgcac caataccaat atttttcgta tctagtacat atgcttccaa
    59581 ttcttcttgc tgttgtgcaa ttaaacgaac cacattatat gtcccgctca atccactgga
    59641 aattgttacc gcaagtacct tttcataacc gtcttttttg atttgctcaa agatttctgt
    59701 gatcgactca ccatccggca aagatgtacc agggatttcc tctggcaaac gttgatagat
    59761 ctcttcaggg gtaatgtcaa ttttatctgt atatacctga tctttataaa taattttcaa
    59821 tgggatcata tacatctcgt atttttttat caattcttct ggaacatccg tacctgaatc
    59881 tactaaaatt gcgattttat tgtttttcat ttgttttctc ctcttttaaa tattcggttt
    59941 ctagaacaat cgttttttcc gcaagaagtt tcattgcaaa cgataaagtt gccgctctta
    60001 cagcaatata ttctacaggg gttgcctggt cgaatttttg cggatgagat ttaccttgtg
    60061 ctaattggct gaccatccac acagctgctt cttgttcatc gcaaaataga ttataggctt
    60121 cacgaattcc tactgttttt ccttggaaca atatcccttc tttgatttca ggaatagtca
    60181 atacttgttt caacgtagtg attgcaagta aaaatgctac gtgttctttg ttatatcttt
    60241 ttttaactgg tgctggtatc aaacctagtt taacgtaatt gtttaccata gaagaggtaa
    60301 gcaatggatg cttttctcct tgtatcacag gtgaaagata acgatcaact aacgttctga
    60361 cttggtccat ataaagttct aagtcaggga gttcttccca gtgaggcaga cggaatgttt
    60421 ctagttcctt cccccactcc attaattgct gacgcatttt ttccatcata accgctcctt
    60481 ttttcatttc ataaacatga tatcagactt atgataaaca ttcaatctag ttgctttaac
    60541 taggtgattt gattagcggg attccatgaa aacaaaaaac aagaaggcta agccttcttg
    60601 tcttttatcg ttaagaacag gagacccgcc ggggagacgg cgggaaagga gtaaaaaatg
    60661 aaaaaagtgt tagggtttgt tctcttgcaa gaactttgtt tgttggtatg cttatataat
    60721 agcgagcaaa tgtgaataat ctatgaatga tttcctataa ggaagtaaag atttttctga
    60781 ttttctttaa atgtagtctt gcattttaaa attcttagtg ctactattat taagtacttt
    60841 ttgtacgggc cgttagctca gttggtagag cagctgactc ttaatcagcg ggtcgcgggt
    60901 tcgagcccct cacggcccat tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa
    60961 agcatttcga aaacatttac gctgttcttt ttttattttt caaaataact agggcttttt
    61021 atttttaact caaagtccac tgtagccctt tggactttga gttaaaaaag aagcataatt
    61081 atgaacagta aagtttctaa tataacaata attcgagaag ctttgcaaaa tcatgctgcc
    61141 aatgaactct ctaaa
//
LOCUS       DAYYBG010000005        60236 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000005 DAYYBG010000000
VERSION     DAYYBG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.005"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACHAUN_000305"
     CDS             <1..288
                     /locus_tag="ACHAUN_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459040.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACHAUN_000306"
     CDS             347..787
                     /locus_tag="ACHAUN_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459041.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACHAUN_000307"
     CDS             799..1467
                     /locus_tag="ACHAUN_000307"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8459042.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACHAUN_000308"
     CDS             1531..2154
                     /locus_tag="ACHAUN_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8459043.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACHAUN_000309"
     CDS             2210..2596
                     /locus_tag="ACHAUN_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8459044.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACHAUN_000310"
     CDS             2809..4200
                     /locus_tag="ACHAUN_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8459045.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4811..5179
                     /locus_tag="ACHAUN_000311"
     CDS             4811..5179
                     /locus_tag="ACHAUN_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8459046.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5359..6300
                     /locus_tag="ACHAUN_000312"
     CDS             5359..6300
                     /locus_tag="ACHAUN_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8459047.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6369..7100
                     /locus_tag="ACHAUN_000313"
     CDS             6369..7100
                     /locus_tag="ACHAUN_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8459048.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7244..9424
                     /locus_tag="ACHAUN_000314"
     CDS             7244..9424
                     /locus_tag="ACHAUN_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8459049.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9444..9926
                     /locus_tag="ACHAUN_000315"
     CDS             9444..9926
                     /locus_tag="ACHAUN_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8459050.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9942..10703
                     /locus_tag="ACHAUN_000316"
     CDS             9942..10703
                     /locus_tag="ACHAUN_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8459051.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10834..12168
                     /gene="murC"
                     /locus_tag="ACHAUN_000317"
     CDS             10834..12168
                     /gene="murC"
                     /locus_tag="ACHAUN_000317"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8459052.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12475..12960
                     /locus_tag="ACHAUN_000318"
     CDS             12475..12960
                     /locus_tag="ACHAUN_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8459053.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12953..13636
                     /locus_tag="ACHAUN_000319"
     CDS             12953..13636
                     /locus_tag="ACHAUN_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8459054.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13762..16407
                     /gene="polA"
                     /locus_tag="ACHAUN_000320"
     CDS             13762..16407
                     /gene="polA"
                     /locus_tag="ACHAUN_000320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8459055.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16452..17288
                     /gene="mutM"
                     /locus_tag="ACHAUN_000321"
     CDS             16452..17288
                     /gene="mutM"
                     /locus_tag="ACHAUN_000321"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8459056.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17252..17881
                     /gene="coaE"
                     /locus_tag="ACHAUN_000322"
     CDS             17252..17881
                     /gene="coaE"
                     /locus_tag="ACHAUN_000322"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8459057.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17933..18265
                     /locus_tag="ACHAUN_000323"
     CDS             17933..18265
                     /locus_tag="ACHAUN_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8459058.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18577..19068
                     /gene="nrdR"
                     /locus_tag="ACHAUN_000324"
     CDS             18577..19068
                     /gene="nrdR"
                     /locus_tag="ACHAUN_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8459059.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19091..20464
                     /locus_tag="ACHAUN_000325"
     CDS             19091..20464
                     /locus_tag="ACHAUN_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8459060.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20464..21390
                     /gene="dnaI"
                     /locus_tag="ACHAUN_000326"
     CDS             20464..21390
                     /gene="dnaI"
                     /locus_tag="ACHAUN_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8459061.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21519..21959
                     /locus_tag="ACHAUN_000327"
     CDS             21519..21959
                     /locus_tag="ACHAUN_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8459062.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22103..22804
                     /locus_tag="ACHAUN_000328"
     CDS             22103..22804
                     /locus_tag="ACHAUN_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8459063.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            22801..23922
                     /locus_tag="ACHAUN_000329"
     CDS             22801..23922
                     /locus_tag="ACHAUN_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8459064.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            23937..25169
                     /gene="pepT"
                     /locus_tag="ACHAUN_000330"
     CDS             23937..25169
                     /gene="pepT"
                     /locus_tag="ACHAUN_000330"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8459065.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25301..26209
                     /locus_tag="ACHAUN_000331"
     CDS             25301..26209
                     /locus_tag="ACHAUN_000331"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8459066.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26244..26516
                     /locus_tag="ACHAUN_000332"
     CDS             26244..26516
                     /locus_tag="ACHAUN_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8459067.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26527..27573
                     /locus_tag="ACHAUN_000333"
     CDS             26527..27573
                     /locus_tag="ACHAUN_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8459068.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            27824..30433
                     /gene="clpB"
                     /locus_tag="ACHAUN_000334"
     CDS             27824..30433
                     /gene="clpB"
                     /locus_tag="ACHAUN_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8459069.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30584..31270)
                     /locus_tag="ACHAUN_000335"
     CDS             complement(30584..31270)
                     /locus_tag="ACHAUN_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459070.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31248..31742)
                     /locus_tag="ACHAUN_000336"
     CDS             complement(31248..31742)
                     /locus_tag="ACHAUN_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8459071.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(31762..32982)
                     /locus_tag="ACHAUN_000337"
     CDS             complement(31762..32982)
                     /locus_tag="ACHAUN_000337"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8459072.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33129..34964
                     /gene="lepA"
                     /locus_tag="ACHAUN_000338"
     CDS             33129..34964
                     /gene="lepA"
                     /locus_tag="ACHAUN_000338"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8459073.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35160..36071
                     /locus_tag="ACHAUN_000339"
     CDS             35160..36071
                     /locus_tag="ACHAUN_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8459074.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(37011..37970)
                     /locus_tag="ACHAUN_000340"
     CDS             complement(37011..37970)
                     /locus_tag="ACHAUN_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8459075.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            38133..38384
                     /locus_tag="ACHAUN_000341"
     CDS             38133..38384
                     /locus_tag="ACHAUN_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459076.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            38497..38739
                     /locus_tag="ACHAUN_000342"
                     /pseudo
     CDS             38497..38739
                     /locus_tag="ACHAUN_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38829..39068
                     /locus_tag="ACHAUN_000343"
     CDS             38829..39068
                     /locus_tag="ACHAUN_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459077.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            39215..43498
                     /locus_tag="ACHAUN_000344"
     CDS             39215..43498
                     /locus_tag="ACHAUN_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8459078.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            43597..43962
                     /locus_tag="ACHAUN_000345"
     CDS             43597..43962
                     /locus_tag="ACHAUN_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459079.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            43964..44416
                     /locus_tag="ACHAUN_000346"
     CDS             43964..44416
                     /locus_tag="ACHAUN_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459080.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            44865..46340
                     /locus_tag="ACHAUN_000347"
     CDS             44865..46340
                     /locus_tag="ACHAUN_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8459081.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            46363..46674
                     /locus_tag="ACHAUN_000348"
     CDS             46363..46674
                     /locus_tag="ACHAUN_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459082.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            47035..48291
                     /locus_tag="ACHAUN_000349"
     CDS             47035..48291
                     /locus_tag="ACHAUN_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8459083.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            48288..48833
                     /locus_tag="ACHAUN_000350"
     CDS             48288..48833
                     /locus_tag="ACHAUN_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459084.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            48834..49334
                     /locus_tag="ACHAUN_000351"
     CDS             48834..49334
                     /locus_tag="ACHAUN_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459085.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            49693..49971
                     /locus_tag="ACHAUN_000352"
     CDS             49693..49971
                     /locus_tag="ACHAUN_000352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459086.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            49991..50335
                     /locus_tag="ACHAUN_000353"
     CDS             49991..50335
                     /locus_tag="ACHAUN_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459087.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            50354..51265
                     /locus_tag="ACHAUN_000354"
     CDS             50354..51265
                     /locus_tag="ACHAUN_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8459088.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            51344..52336
                     /locus_tag="ACHAUN_000355"
     CDS             51344..52336
                     /locus_tag="ACHAUN_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8459089.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            52333..52551
                     /locus_tag="ACHAUN_000356"
     CDS             52333..52551
                     /locus_tag="ACHAUN_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8459090.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            52564..52974
                     /locus_tag="ACHAUN_000357"
     CDS             52564..52974
                     /locus_tag="ACHAUN_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8459091.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            53026..55554
                     /locus_tag="ACHAUN_000358"
     CDS             53026..55554
                     /locus_tag="ACHAUN_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8459092.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            55570..57780
                     /locus_tag="ACHAUN_000359"
     CDS             55570..57780
                     /locus_tag="ACHAUN_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8459093.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            57788..58879
                     /locus_tag="ACHAUN_000360"
     CDS             57788..58879
                     /locus_tag="ACHAUN_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8459094.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            58900..59442
                     /locus_tag="ACHAUN_000361"
     CDS             58900..59442
                     /locus_tag="ACHAUN_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8459095.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            59637..59894
                     /locus_tag="ACHAUN_000362"
     CDS             59637..59894
                     /locus_tag="ACHAUN_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459096.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            59908..>60236
                     /locus_tag="ACHAUN_000363"
     CDS             59908..>60236
                     /locus_tag="ACHAUN_000363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459097.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
BASE COUNT        20350 a         9907 c        12914 g        17065 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     4801 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     4861 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     4921 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     4981 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5041 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5101 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5161 tgcggttaca ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc
     5221 gaagtctttt taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta
     5281 tacacaagca aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa
     5341 gaaggaagga agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc
     5401 gaagaaccag aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa
     5461 ggtcgtattc tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac
     5521 acgactgcct atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag
     5581 atcgatccaa gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt
     5641 gtaagtttaa agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca
     5701 ccgcaagaat ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat
     5761 gattatgaat atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt
     5821 cgagagcttc cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg
     5881 acctactgta ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga
     5941 ttcaaagatg tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc
     6001 caaggagaat tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc
     6061 aaccaagtag ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga
     6121 tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa
     6181 gaaaaacatt taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt
     6241 caagaccatg gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag
     6301 aaaacaatag ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg
     6361 tgatccacat gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt
     6421 ttgaaaaact tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc
     6481 tcaatgatcg aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag
     6541 aaacaggagc catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat
     6601 caccggagga ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc
     6661 tgattcaccc aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat
     6721 cgagatattc agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa
     6781 attatctttt tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc
     6841 taagagactt tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga
     6901 caattgtatt tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc
     6961 tctggattca ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc
     7021 atggggttgt ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag
     7081 gaccttggca tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct
     7141 ctttttattt ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat
     7201 gatagaataa agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa
     7261 ccagacgatc attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac
     7321 tactgtactt aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa
     7381 agaaatgact ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta
     7441 tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa
     7501 actgactccc gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga
     7561 tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg
     7621 acttgaacca ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc
     7681 agaaaaatat attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc
     7741 tcatgagatt attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc
     7801 ttatacgtat aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa
     7861 aggcgtatat gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg
     7921 aatattagct actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga
     7981 tgaagcagca attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc
     8041 ttcttcattc gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat
     8101 cgagcgagaa ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt
     8161 cggcgaaaac ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg
     8221 atttgatccg gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt
     8281 tttagcaatc gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc
     8341 tggaccagct tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa
     8401 tggtacagcc agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca
     8461 taaaacctat tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat
     8521 tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg
     8581 tcgcctgcaa gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg
     8641 ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga
     8701 aacagcggtt aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca
     8761 tatatcagga ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg
     8821 agaattcacc gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga
     8881 acaagcaatc ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat
     8941 ccatcccgaa agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa
     9001 agagcttggt acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc
     9061 tgaaagccta tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc
     9121 tggacgagat atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat
     9181 ggaagacttg aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg
     9241 cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa
     9301 aaaattcgtc aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat
     9361 agaacaagtc gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga
     9421 gtaacaatga ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga
     9481 tatcattaaa aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac
     9541 gtacaactgg cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat
     9601 ttgaagagtc agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc
     9661 atcttcatct agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga
     9721 aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt
     9781 attatcgatg ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca
     9841 aatatcgttg tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact
     9901 ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga
     9961 ggaatctaaa agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt
    10021 ttgaggaact gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg
    10081 ataaagcagc acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg
    10141 tggggcatgc gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag
    10201 atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg
    10261 ataaggcttt tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac
    10321 tggagattct tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga
    10381 cactgcaacg caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt
    10441 tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta
    10501 cagcaaaaga actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag
    10561 aaatggaacg tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc
    10621 ctcacattga tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa
    10681 aaacggattt ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt
    10741 tggttgaaat tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa
    10801 agaatggacg ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat
    10861 tttgttggta tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt
    10921 ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa
    10981 gcaaatatca cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt
    11041 gcaggaaatg catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg
    11101 gaagtgattc gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct
    11161 gtaacaggct ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt
    11221 gtccgtccaa caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa
    11281 ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat
    11341 gcaatcatga ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta
    11401 tttacagctt tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac
    11461 gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa
    11521 aacgatgata tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc
    11581 tatcatgggg atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc
    11641 ttaaatgctt taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt
    11701 gcagaagaaa tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca
    11761 gacatgaccg tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat
    11821 ggcgcacgcc aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt
    11881 acgcgtacga ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt
    11941 tacttatgcg atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac
    12001 cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg
    12061 ttagattatc atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa
    12121 caagcgtatg aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc
    12181 agtgtcctta taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt
    12241 tgcaacttat gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat
    12301 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
    12361 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
    12421 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
    12481 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
    12541 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
    12601 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
    12661 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
    12721 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
    12781 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
    12841 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
    12901 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
    12961 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
    13021 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
    13081 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
    13141 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
    13201 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
    13261 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
    13321 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
    13381 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
    13441 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
    13501 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
    13561 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
    13621 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
    13681 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
    13741 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
    13801 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
    13861 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
    13921 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
    13981 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
    14041 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
    14101 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
    14161 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
    14221 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
    14281 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
    14341 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
    14401 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
    14461 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
    14521 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
    14581 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
    14641 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
    14701 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
    14761 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
    14821 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
    14881 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
    14941 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
    15001 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
    15061 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
    15121 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
    15181 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
    15241 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
    15301 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
    15361 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
    15421 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
    15481 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
    15541 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
    15601 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
    15661 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
    15721 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
    15781 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
    15841 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
    15901 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
    15961 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
    16021 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
    16081 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
    16141 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
    16201 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
    16261 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
    16321 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
    16381 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
    16441 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
    16501 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
    16561 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
    16621 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
    16681 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
    16741 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
    16801 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
    16861 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
    16921 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
    16981 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
    17041 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
    17101 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
    17161 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
    17221 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
    17281 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
    17341 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
    17401 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
    17461 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
    17521 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
    17581 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
    17641 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
    17701 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
    17761 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
    17821 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
    17881 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
    17941 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
    18001 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
    18061 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
    18121 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
    18181 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
    18241 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
    18301 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
    18361 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
    18421 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
    18481 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
    18541 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
    18601 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
    18661 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
    18721 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
    18781 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
    18841 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
    18901 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
    18961 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
    19021 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
    19081 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
    19141 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
    19201 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
    19261 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
    19321 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
    19381 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
    19441 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
    19501 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
    19561 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
    19621 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
    19681 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
    19741 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
    19801 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
    19861 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
    19921 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
    19981 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
    20041 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
    20101 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
    20161 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
    20221 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
    20281 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
    20341 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
    20401 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
    20461 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
    20521 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
    20581 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
    20641 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
    20701 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
    20761 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
    20821 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
    20881 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
    20941 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
    21001 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
    21061 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
    21121 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
    21181 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
    21241 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
    21301 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
    21361 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
    21421 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
    21481 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
    21541 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
    21601 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
    21661 gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa
    21721 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
    21781 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
    21841 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
    21901 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
    21961 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
    22021 taaggagata tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata
    22081 attatctaga aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt
    22141 gggtgacttt gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt
    22201 acccgttgca ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca
    22261 aggtccttat gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac
    22321 cgtacgcctt gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac
    22381 gattgcgggt atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca
    22441 acgcttaagc ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg
    22501 atcttggctg caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa
    22561 aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat
    22621 ggagctgatg ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg
    22681 gacaagagag cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca
    22741 agaggcacgt caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc
    22801 atgacaatca gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta
    22861 gctgaagaag gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga
    22921 gtaatgatga cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc
    22981 gatttgctga ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat
    23041 cagccacaag aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca
    23101 cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt
    23161 attgaagtgg agagttattt agtaaagact catgagattc attataaaaa actggccgtt
    23221 tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt
    23281 acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg
    23341 gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca
    23401 aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca
    23461 gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg
    23521 tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa
    23581 caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa
    23641 tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca
    23701 atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat
    23761 atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag
    23821 caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt
    23881 ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt
    23941 acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa
    24001 caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag
    24061 aattagaaga gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg
    24121 gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg
    24181 atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg
    24241 agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa
    24301 atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag
    24361 cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc
    24421 cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg
    24481 gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg
    24541 gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc
    24601 aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag
    24661 caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg
    24721 aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata
    24781 tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc
    24841 aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat
    24901 attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag
    24961 caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt
    25021 caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc
    25081 acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa
    25141 aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt
    25201 tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt
    25261 gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt
    25321 ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta
    25381 tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc catcgattga
    25441 tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg
    25501 tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca
    25561 tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct
    25621 caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg
    25681 tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc
    25741 agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt
    25801 ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca
    25861 ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac
    25921 aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc
    25981 cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac
    26041 gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc
    26101 tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt
    26161 atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt
    26221 aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc
    26281 ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa
    26341 ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt
    26401 tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta
    26461 taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag
    26521 caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt
    26581 gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa
    26641 ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa
    26701 atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg
    26761 aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca
    26821 ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga
    26881 gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta
    26941 gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt
    27001 atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc
    27061 agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt
    27121 ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa
    27181 accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt
    27241 gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt
    27301 ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct
    27361 gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca
    27421 gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc
    27481 tatggatttg tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa
    27541 gtaaaaaaag agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat
    27601 caaatcaaag aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat
    27661 atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa
    27721 actatgtgta tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca
    27781 aagaaataag tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat
    27841 gactacgacg cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca
    27901 tcaagatatc gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg
    27961 aaatttctat acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct
    28021 actggatgag tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca
    28081 gaatttcttt cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt
    28141 tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt
    28201 ttatttgaat aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag
    28261 aggaggagaa agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata
    28321 cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga
    28381 tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct
    28441 gataggagaa cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt
    28501 acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc
    28561 attgatcgct ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa
    28621 agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt
    28681 aggggcagga aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc
    28741 tcgcggtgaa ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga
    28801 aaaagacaaa gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga
    28861 agatacgatc agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa
    28921 catccatgac aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg
    28981 gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga
    29041 aatgaactct atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat
    29101 cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt
    29161 gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac
    29221 agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca
    29281 cgaattagaa gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg
    29341 tacgatacct caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga
    29401 gattaagatg gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt
    29461 aacgggtatc cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa
    29521 tgaaacactt cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc
    29581 ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt
    29641 cctaggacca actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt
    29701 tgactcagaa gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt
    29761 gtctcgtcta gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac
    29821 ggaagctgtc agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca
    29881 tcccgatgtc ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa
    29941 agggcgagta gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca
    30001 gttactgctg gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt
    30061 caacacgcta ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat
    30121 tttatttaca ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca
    30181 attggcacaa cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac
    30241 gtggattgct gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat
    30301 cactaaggaa gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa
    30361 atcaaaagta acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga
    30421 agaaatcgtc taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt
    30481 gtcagacctc cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc
    30541 cgaaatttct tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt
    30601 tttctaattt ataactttca aagtaacgct catctttttt actttgagac catgcaaaca
    30661 atcccattaa ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg
    30721 ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta
    30781 agaactggtc gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg
    30841 caaagccgaa taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca
    30901 agtaaccaat caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga
    30961 ttttgtccca aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga
    31021 tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt
    31081 tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg
    31141 tgattccttg agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa
    31201 cgaatacacc aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa
    31261 gtccctccat ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact
    31321 tttccgttca atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca
    31381 gctgtttctt gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca
    31441 gtatataagc cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc
    31501 ccggcatcga tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt
    31561 tctgtgtcag caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct
    31621 tcattttctt gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact
    31681 aactgttggt tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc
    31741 atctaaacat catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat
    31801 gatacgctct agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa
    31861 ataagaagca cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac
    31921 ggattttcct ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc
    31981 gaagacacca ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc
    32041 tgttccaaca aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa
    32101 atatggttcg atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt
    32161 tttataaata tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca
    32221 taagccattt cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc
    32281 agctacccag tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc
    32341 tgtgcgctca tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa
    32401 taaagcacca tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc
    32461 aactggtaat cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct
    32521 gattttaaag gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat
    32581 actgtaagga tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac
    32641 gataattaga tcttgcatca atccatggtt agcatattct ggataagttc caactccagt
    32701 cacaccaccc attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat
    32761 ttgagagatc agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg
    32821 ttttttggaa cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc
    32881 agtcacttgg aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac
    32941 cggttttaat tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat
    33001 tagcttgata acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa
    33061 aactctttaa attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag
    33121 gagacgctat ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga
    33181 tcatcgctca tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc
    33241 acactgtgac gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg
    33301 aacgtggaat cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag
    33361 aaacatatat ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct
    33421 ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg
    33481 aagcacaaac attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag
    33541 tcatcaataa aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag
    33601 acgtaatcgg aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga
    33661 ttgaagatat tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg
    33721 caccgctaaa agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa
    33781 atgtccggat cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg
    33841 gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact
    33901 ttttgatggt cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac
    33961 gagtagggga tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc
    34021 gaaaaatgaa tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg
    34081 atttgcggga agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag
    34141 aaacctctca agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg
    34201 atgtcgtcca agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat
    34261 ccgttattta ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag
    34321 attttcctga acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca
    34381 tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat
    34441 tcattacgat ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt
    34501 ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat
    34561 tggattatga aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga
    34621 atggagaaaa agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg
    34681 gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa
    34741 tccaagcagc tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa
    34801 acgtcttggc taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac
    34861 aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct
    34921 tcatggccgt tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa
    34981 aaacctatta aataaacctt tttgagtaga tgcaacactg aggattatct gtataaaaga
    35041 ttttaaactg tcatttggtt atcacggcga tactaaaatt aacaaaattg gaatattttt
    35101 cttgaagatt tttagctgtc agtatatgat gaaagcatct cataggaggt gcttttttca
    35161 tggttgataa ttcaagtgga agggttctta aatctctaag aaaagagaaa aaactatctc
    35221 aaaaaaagtt agctgattta gcaggtattt cacaaagtac tttagttaaa tatgaaaaag
    35281 ggtcaagaaa aatccctaaa gacgtagata atactctaag caagattctg aatatagaaa
    35341 ctctaataaa agatgaggaa gataagattg agattctcat cggtaaacta attgcataca
    35401 gagatatgaa taagttactt aacaaagagt tagctgataa tataggaatt agtgaagtat
    35461 tactcagtta tgtgttaaat agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg
    35521 atatttttct attaagtaat gaaaaagaaa ttttgaagga gattaatcgt gatagtgaga
    35581 tatttagttt atcaaaagac gataaaattg ttatgggaaa aagaatacga gaagtccgaa
    35641 agaaccggga agagacgctt gaaaaatttg gtaaaaactt taccatctat acaggcaaaa
    35701 atgttataag tagatgggaa aaaggtatta atattccaga tattgaaaaa ctaatgaata
    35761 ttgcctatct tggtaaggtc actgttcctt accttatgta cggagaagat tataagaata
    35821 tacttccaaa agatgaaaga gttagcgatt ttaaaaagat aaattctttt agtatgggat
    35881 taaggatgag gaaaattagg aaagattatt atctagaaag ggaggagttt ggaaagcttt
    35941 tttctccttc aatttcaaaa tggtccatag ataggtatga gaatggtaga gatataccca
    36001 atactaatcg aattatccaa tatgcttata taggaaatct ttctttagaa tttttgattt
    36061 acgggatata aaaatagttt acgtcgatac ttattaagtt atcgacgttt tttattatag
    36121 aaaaaattta taaaaggagg tttcttgata tgataagaaa caaaaagggt ttgttgaaag
    36181 ttgctttact ggcagctttt ttctaagtaa ctttttgaca agctcaatgg caacacaagc
    36241 ggaagaagta atacaagaac taactgttat gaataacagc tagataaaat gggctatgta
    36301 tgttatccaa caatttagta atcactagaa gcctagcgaa agttcgcttg ataagttata
    36361 gatgagtatt gatttgtagt gtttccaaat caaaagggta cagagccaca tgaaatatgt
    36421 tgtcctctgc agcgtcagga tgggaatcca taccatggag attcggagat ctgtgaggga
    36481 atacttacgt ggttaatatg tcagttgtgt tggacgtagt aatggtcata gtgggtagat
    36541 tgactgaatt cttatatgag aaaatgaaac caacaagaag atagggttat ttatacagaa
    36601 ttggggatct tttctggaaa agtaatcgga taatgcgaga ttcgtacggt cttttttaaa
    36661 tttctctaaa aaaaacggca tcgaatgggt tgggtgattg ataagcaatg aatcatagac
    36721 tgatatagaa taaaaaatga ttctatttat ctttttacat ctatcgtttt ttcaagtagc
    36781 cgcaaattat atcagcattc aacaattttt gtatacaagt tctagtaaaa ggatctgaag
    36841 aaaaagttgg ttgttgtgaa gataaaaaaa gaatcaggtt ttactgagtt tttcttcaaa
    36901 atttgtgtta ttaggttagg acaaagataa gaagaagtat gaagatttaa aaaaggaatg
    36961 aggaaaagat agtaatttcc gattattgga gtttttttat ttttcgtttg tcaaattaag
    37021 ttagacaaat catcttttgt gacgtaactc aaaaatactt ccattggtgt ccgatagtgt
    37081 aatgattttc taggaatatg atttcgtttc gatgccaccg attggataaa ggtttcagat
    37141 acaccattaa aatccatagc tttatacaaa ccatctttac gtaataagcc atttgaatgt
    37201 tcattcaatc ctcgttgaga aggtgttcct ggatccgcaa aataaattga aatatcgttg
    37261 gtattactaa tctttttcca atttgagaat tcttttccac aatcaaatgt gatggattta
    37321 aataagtttt ttgggacaca acgaaaccat tgattcagcc tattttcaat gtctttagcc
    37381 tgtctgcctt cgggtttcaa ggtaatgatg actttagata gccgttcaac taatgtgatg
    37441 accgcgcttt tatgttttcc acccacgata gtgtcacctt ccaggtgacc aaattcatga
    37501 tcaaactggg tatagtctgt ctttctgtct cggattgacc ggcgaaaccc ttgttttcct
    37561 cttttttctt tatgaccgtt tggttttctt tttcccttca ttggtaaatc gtgagtgtca
    37621 aaggttttat ctttaaacat tcgatacagt gtgcgtaccg aacaagcaat aggaaactct
    37681 gctcgaccaa taaggacatc aggagcccac ccttgaacaa cctttttttg aatgtattcc
    37741 tgttgctcat caggtaaaac aatcggatgt cttccgcagt tacgtttgtt tttcttatat
    37801 tgtttatagt agctcattgc gcttccacca gatttaagaa aacgatagac cttataaata
    37861 gtttgtcgag aacgttgtaa cagattggct acttttgaaa caggttgtga ttgattgaaa
    37921 taagcttcta tcattacgag ttcagttggt gtaagatggt tataggtcat ttgtactcac
    37981 tccttataat tctttcgtcg gaactatttt gagtgtagca caaatgactt tttttagttg
    38041 tctagcttaa ttttacaatc ggcgatttat aaagaagaag cgaataagtt gtgattccta
    38101 gatttcgcgc gtgattaatt aatgagcaaa aaatgcggaa agttttagtg tttaatacac
    38161 gaggacaaag tttcttaggt attgaaggca cacgtgtaac ggttgatggg tggtgtgatt
    38221 gtttcgaggt tggaaaacat gttgaactta tggcatttac tgagctaaaa gataagaaca
    38281 acgtggaatt tttcgagagt gatattggtt gggacgatca tgaagaagtt tatggaaaat
    38341 ttattttgcg gaagggaaat tcttatacga atggggaagc ataagtagcg atttgttaga
    38401 gatcattgat aggattgaaa ttgtcgggaa tatttatgag aatctagagt tatttgaggt
    38461 gatagaatga gtgatggttt tgaacaagta tcaataatta aaaatcaagt tcgggagatt
    38521 cttagaaaaa aaggctattt ggtagatagt tattttgaag gagattacga aacatgggta
    38581 ggtgtctatg ctcgtccaga gaataaacct acctatttag atcctacgac gtcagaagat
    38641 gcctatttac aaaataggta tcgagtagat ggttttaagc aagactttgc ggagtggttt
    38701 gaatgggaaa ttgaaaacgg agaagtaaaa gaagaataag agtacttgtt gtatcaaaat
    38761 aagccagtag aacaaccaaa ccagtagcta gtaatgacaa taatactgag gaatcggatc
    38821 attatggtat gattgatatt tctcttccag aagatcttaa gccagatcct gtaacaattg
    38881 gcaatagtgt catgattttt gattcattag atgaagctat gtcatatggt tggaaagaaa
    38941 ttaaggatga aggtagtaat tggtatggca tgagatttgg tgatggtgaa gttgccaaaa
    39001 aagctggtat tccaattggg aaatggacag ttgatttcca cgaagcaggt tctttttttg
    39061 ttctgtaatt atagtgggag ggtaaaatca ttgatgaaat gtgttcagat aagagcgcca
    39121 gataaaaata gaagagacag ttcacgtcgt tatcactttt ggtaacggcg ttttgtttta
    39181 ttatataaaa ttctcaggaa aaggaggaat tcttgtgtat gcaaaaacga aaatgaattg
    39241 gttgaaagca ctcgcattgg tggctttttc tcaattctgt ttcttggtgg gaactgctca
    39301 agcacatgca gaagaaacga cggctataca acctacgatg gaagccattg cccaagtcgc
    39361 ttctgaagtg acagaagtcc aaccagcaga agccggggtc ccagctggtg aagccactgt
    39421 tgaacaggcc cctgtgacag aagcgccaac ggtcaattcc aaaacgactc tcgatcagac
    39481 gattggggaa acacaacacc cagggaatag tcctggtact tcacaaccag ttgaagaaac
    39541 tgcctcaatt acaacccctg cttcagaaac gtccactcta gaaggagaaa aagctgccga
    39601 agcaacgcct tctgtgcagc cagagtcttc tgagaaacaa gcggaagaaa agaaacgaat
    39661 agaagaaaca gtggaacaac cagcggctac tgctgacaaa gaaggaaaaa atgaggaaaa
    39721 cgttgaaaaa acaatctctt caaccgcagc tagtcaaaat caaccaacga aaccaaacaa
    39781 agaatctacg gctagtattg aaaatcaaaa gaaaaaagag cagaacattc caaaaacagg
    39841 tacacctaca ctgttagtaa cagtaccaac caatcgttcg gttcgtgttc gccgtgcaac
    39901 caatgcagct gttttaggtg gtcggaaaat tgattggggc gaaaatattg gtgttacttc
    39961 tagtccgaac aatctaaact tcactttaaa ttatatggtt cgttttgaga ttaatggaca
    40021 agtggcgttt tgtattgagc ctggtgtctc agccgcagaa ggtcaagttt atacagctac
    40081 tgctttgaaa aactatttga aaaattcaaa tgtacggaaa aaaacaagct tgatctctta
    40141 tttaggatat gtcacaaata aagacaaatc cgatgaacaa tacattgcgg cacaatttat
    40201 gatttgggaa gctttgggca caaaaatccc aatcaaaaat atccgtgttg attatgatcg
    40261 tcgaaaagca gaaattcaac gtttgattaa taactttggt gaaaaagcaa cttttaataa
    40321 tcaaacacat acggtaaaaa ttggcgaaac gttgaaagta aagaataaaa atgatttttt
    40381 gaaatatgtg aaagacattc aaaaaccaaa aggtgtcaat gtatctattc acggcaatga
    40441 agtatggatt acagtcacaa aagaggcacc ggataaagca aaaattcaat tgaataaaat
    40501 taaagttcaa ggtgcgccat tagcctatcg tcgtccagga gctcaaacaa ttggtgtgtt
    40561 acatccaaca gaacctaatg cgtctgttat taatttacaa attctaaaac aaggaaacat
    40621 tcgtgtgttt aaagaagatg cggaaactgg tgtgaaagca caaggtgcag cttctttaga
    40681 aaatgcggtc tatggtttgt atcaagcaga tgggaaaaag attaaagaaa ttaccttgaa
    40741 aaaaattaac ggcaaagtac aggcagaaat taaagatctt gatcctggca aatatgtttt
    40801 aaaagaaatc aaagcgccaa aaggttatgt gctttctgat gaagcaattg aaattgttgt
    40861 tgaacctgga aaaactgtca atgcgaccgc aaaagatcaa gtgatcaaag gaaccattga
    40921 tttagtgaaa gttgctaata aagatttagt cgattcaacg aatccagata acaaaccaaa
    40981 attagctggc attgaaatca ctttgacgtc aaaaacaact ggcaaagtgg tgaaaaccgt
    41041 tgtgacagac aatgatggtt atgctacttt tggtaaaaat accgttgtct ttgatacgta
    41101 cattctttct gaaaccaaag gaaaagaagg gtataaacta tttgaaccac ttgaagtcac
    41161 aatttctgaa aatgggcaaa ctttccatta tgttttagaa gataaagtaa ttgaacaacg
    41221 tcttaaagtg gtgaaagtgg atcaagaaac aggaaaagtg attccacttg ctaatacaga
    41281 attcaagatt tttgatactt tagccaatcg ttatgtcaca atggagattc caaatgatac
    41341 agaaacaacg gatatcttta aaaccaatga aaaaggcttt ttcctaacta acggtacact
    41401 tacttatgga aaagatcgct atcgtttgga agaaatcaaa gcccccgtaa attacgtttt
    41461 gaatcaaaac ccattagtgt tctcgattac caatgataca gaaccgatca aagtgatcca
    41521 ttttgcgaac cgtcaagcac ggaaaagtgt aaaattattc aagtatgaag aatggaacaa
    41581 gaagaaaacg ccgctcgcag gtgtgaactt taccttgtat aacggtaaaa atcaagcaat
    41641 tggtgaatat accactgatc aaaacgggga aattttagtg aaaggtctag ttgccgatga
    41701 ctattacttc ttggaaaatg ccccattaga aagtttccaa ccaaatacag ataaacaagc
    41761 attcacggtt gcattcaaac aaaatgcgcc agatgatggg gaaacgttag tggtttctgt
    41821 acgtaattac ttgattccac caaaactaga aacacaagcg tcaaataaag ccgatggtga
    41881 aaaagtggtt gatccattag aaacgatcgt cattaaagac agtgtttctt ataccaactt
    41941 gtttgtcgga aaagaataca ccatttcagg agtattgatg aacaaggcaa cgaatgcgcc
    42001 aattttacaa gatggcaaag aaatcacaag taccttggcc tttattgcag acaaagtcaa
    42061 tggcacaaaa gaccttgaat ttgtggttaa tgctagtgtt ctgaaaggtc aaacgatcgt
    42121 tgtttttgaa aaacttttcc gtgatggcat tgaagttgca gcacatgcgg atatcaacga
    42181 tgaagcccaa agtgtgaaag tgaataatcc aaccgttcat accacggctg tctttgaaga
    42241 cggattgaca gtcgcagacc caacaggatt gtttacctta aacgataccg ttgaaattac
    42301 cgagattctt ccaggaaaag catacttggt caaagggatc ttaatggata aagcaaccgg
    42361 tgaacccttg cttgtgcatg gaaatacggt agaaacgagt gttggcttta ttgccaaagc
    42421 agaaaatgaa acggtgatcg taccatttac ctttgatttg acaggtataa atggtcgtga
    42481 aatcgtcgtg tttgaaagtc tttttagaaa tgatgaagaa ctagcaaacc ataaagacat
    42541 caacgacaaa gggcaaacgg tacgagtaac taacccaagt gtacgtacaa cggctacaca
    42601 taaacccact ggcttgaaag aggttaatcc attaacgaaa gtcaccatta cagacgctgt
    42661 gaaataccac gatttggtcg ttggaaaaac atacacagtc aacggtgttc taatggacaa
    42721 agtaacggaa aaaccagtgc tagtagatgg aaaagaagtc acaagtacct taacgtttgt
    42781 ggcagaaaca acggatggta cagtcaatct agactttatt ctagacgcac gtgcgttgcg
    42841 aggcaaagaa gtcgtggtct ttgaagatct gtttagagaa ggcgtattat tggcttctca
    42901 cgcagatatt accgacaatg accaaaccat caaaatcagt aatccaacca ttacgacgaa
    42961 agcgacgaat aaaaatggcg gaaaagagat cttggccttg ccaaatcaaa ctgtgcttga
    43021 atgggtcaaa gtagacggtc ttgcgattgg tcaaatctat cgcttaattg ttcaaggcta
    43081 cttaacacca gacggcacac catttgaagg cactcatgcg gaaaaagtct ttgcggccga
    43141 taaagaaatg atggaattct tatttgaatt cttagtggac ggtcgcaatc ttgcaggaaa
    43201 aggcttgagt tttgcggaat ggctccaagc gaaaacggaa gatgagaaag aaacgtttga
    43261 agaagtggcg aaacacaatg tagatctaac aaacaaagat caaacggttc aaattgtgga
    43321 agcgccaaaa ccaccgcaac cagtgaaacc agaacaacgg gttaaaccag ctactgttgt
    43381 accaatgttg ccaaaaacag gtgaacaaac aagtagtata cttatggttc ttggattagt
    43441 agtattagtc attgctttag gtataacact ttataaagta cgtaaagcca aagattagtc
    43501 atgaggaaca agcggttagg atttttaacc gcttgtttga ttgtaatgta gaaatattta
    43561 gaatttattt tggtttttaa gaggaggaaa aactttatgg cagaaaaagc attgcgtatg
    43621 aagaaagagt ttttaggtca agtattttta ttgggttcac gggaacctaa tccaccaaag
    43681 gaaattaatg gtgtgtctgg gcgatatgtt caattacaag cagagtgttc gcgagaagaa
    43741 cagcctgctt tttttgttta tctcacagct gaagaggata aaagattacg attaccagaa
    43801 aaagaggtgc gtatttcatt tgaagaagct tattttgttt ttcgtaaaga acgagtgaat
    43861 agtcgtgaaa caaatgatat gggacaaaca gagcgtcgaa caactggcta tcaagatgtt
    43921 ggtgtctttt taactggtgt aaaaattggg gaggttttgt aagatgggat taaaatttcc
    43981 aaataattat gacattacag aagcattaga tgaaacttgt ttaggagccg tacgctttgg
    44041 cgcattagaa gagtctgatc gtgtatttga taatgaaaca ggcgattata cgggagaaat
    44101 tcgatcacag cgagcacaat tagtttttgg aaatccagat gaaccaaaga ttgcacgacg
    44161 agttaaattt ccagcagatg tagatttgac tggttttaaa cgacgtgatc ctgtgaaatt
    44221 aattgatcca gaatatcatg tggtatatat tccagcacaa gatggcaatc gtgaatattc
    44281 agatttagtg atttatgcga aaggattaga gaaagtagaa gctccaacaa ataatcctaa
    44341 tatgaaacaa tcacaagaaa aaggtttaga tcaaaaacaa ccaagtgttg attctaagga
    44401 aaaagagaaa aaataaggtt tttttgaaag aataggaagg agtcttccta ttctttttat
    44461 ctaagataga aggaagggga gattttgaat tcagataaaa ccaatcaaat actttgagag
    44521 attgtgcttg ttatcaaaat atccaacaat tgattgagta ttttggagac aatccaacga
    44581 tttttgtaca aacaaatgca tatatttgtg cggttattgg gcagtgtatg tttttacgtg
    44641 atcttgttcg tttttcatta ccgtaccagt ggaaacaaga tcatcaggaa gtgatttgga
    44701 ttcgtatagt acgaatatgt aatatgtgtg cacaccctta taaaaatcga ttaaatcttt
    44761 ttcgattatg tttttttact aaatgtgata ttcctgtatt agttgctttt tgtcaaaagt
    44821 ttttagcaca ggaaaagtaa aaacagaaag gaggaatgtg acaattgaaa gctcattttt
    44881 tcaagtatcg tggtcggcga attcgctatt tcaatcgtta tttatggttt tgctattatg
    44941 gtttcttttt gtttccattt ttgattctgt taggttggtt tagttggaaa gtattttatc
    45001 cacgtttaag tacgttttcg aattggcaaa tgtatcttat tcctggaata agtattgtgt
    45061 tgtatttgtt gctggttagt gggttaattt tagggttgat gcattggtcg cgactaaaag
    45121 aaggatattt tgcctctgtt tattggcgtc agttacttgt tcgaatgtta attgacaatc
    45181 ggtttgttta tacaaagaaa caagttagtg agaaacaaac aagggaaaaa atgagattac
    45241 caaaagttta tttttatcaa aagaaacaag aattagtggt ttcttttcct ttagatggtg
    45301 ggcgttttca cgaacgattt ttaaaaatgg gtcatttgtt atctgaagta tttttgcgtg
    45361 atttaattcg tgaagaacga gaaaaagccc gaatcaatta tgtattcctt tcagattctg
    45421 gtcggattcc tattgatcaa tgtatagcag aaaatggacg tattcattta atgaaaactt
    45481 tggattgggt atatgatcaa tcaccaaata tgttgattgc tggtgcaatc ggtggtggga
    45541 aaacctattt attgtattct ttaattcaag cttgtttaag tgttgggaca gtagatattt
    45601 gcgatggaaa agcggcagat ttagcagcat taggtgacgt cgatgtattc aaaggacatg
    45661 tgttttacaa aataaatgaa gaaatgatta tttgtttacg aaatgcatta aaagaaatga
    45721 atcaacggta tgcctatatg aaaaccatga aagatcctaa atatgaacca gggaaaaatt
    45781 atgcttatta tgggttacca ccgcatttta tcttttttga tgaatgggca gctttttatg
    45841 gttcgttgga tatggaagcg agaaatagag tcgataaatt gattcagcca ttagtattaa
    45901 agggtcgtca agcagggatg tacattattt tagcaatgca acgaccagat gcagaatatt
    45961 ttccaagtgg tgttcgggat aatttaacat tccgtatttc tgttggtcga ttgtctccca
    46021 ttggttattt aatgactttt ggagaagatt ttaaaaataa gccattttac aacaaagatc
    46081 aacctggtcg tggatatgct tttccaggat ttgatgtacc tgtagagttt tttgcgccat
    46141 ttgtttacaa agattatcac tttatcaaag aatttgagaa atttactgaa atgattgtct
    46201 atgattttac agtaaaagag gatagtcaag atcaaacgat gggagatgat aatcagttgt
    46261 tacaagaaca actagagcgc caaatgtatt tagcaaaaga tccaaaagga acaagttttt
    46321 ctgatgcgca tattttgtaa tcaactaagg aggtgaagtt aaatggcaga gagttgttat
    46381 tatagagtag ctacagcaat acgaatgatt aatccttctc tttcttccag aacgttttat
    46441 gattggttga atcgtattga acaagtaact gattaccgct ttttgcgcaa agaacgtgtt
    46501 tttacaggaa aagttattaa tcaagtattg ctaacgaaaa aagatataga acgattgact
    46561 cgattgtatc attatcgagt agatttagaa gaggatttaa ctttgtcaat ttatcgcgtt
    46621 ttttctcctg aaaaatatag tgaaataaca aaattagatc atttaatttt gtagttatta
    46681 tctatcggga gggctctaga cgtagtctgg tagccgcact ctgcctggcg gtgcgcatta
    46741 aggctgcgaa gcatgggcct ataatgcgca ctcgctagca gcgtgtgcag ctcacaaaga
    46801 ggtcgtgcgc ttgcgcacgg cctctttgtg taaagccccc cgatatataa tatggggggc
    46861 agaaataaca taacacatag aaaaaagttt tataaaccct tttgtatcaa gtgattatct
    46921 aaggtcagca gttgtactac ttttcccaaa aaacgagggg gactagttga tatatcggtg
    46981 tttctaaagg gttaaaaaaa atcgattttg ttattttata aaaggagcga gaaaatgaca
    47041 ccgattgagc taaaaagcta cagaaagaag ttaagtgcaa ttcataatat aagtggagat
    47101 aaaaagccgc tatcacaagc aagatttgcg caattgatgg gctgctcaaa agattacatt
    47161 agaaagatgg aacaagggaa aagaaaaatc ccagaggatt ttgaacaacg aatcaaggaa
    47221 cgctttcgat tgtatatgaa aagcggaata gatcttcgag caatgattga ttatttgcgt
    47281 ctttcctttt ttgggacaga ctacaaaaca gtgattcaac gtgtattacg aatggatctg
    47341 acagaatttg aaacacgtcc aacgggcttg tatctttttg actgtctttc tgttcgtggg
    47401 aatatctggg tgttctggca ttcaaaacga actgtaacag aaaatgtact tgtgcaattt
    47461 tcaggacaag gttgccggga atatgaggaa gtcatgcgtg aacaacaaac agattggcga
    47521 acatggctat attccatttg gaaaaatcaa ggaaagttgg aagctggtta tacacgtgtg
    47581 caatgtagcc gattcgatat tgcgttagat gaactttggg gattggaaac ggaagaacat
    47641 ttcgatctct ttgaattact agaaaaacaa aaagcaggtt tattagaaat ggactttaag
    47701 agattccaga atattggcgg ttgtttttta gatgatggct attttcgaag tgaaggatta
    47761 tctttgtatt ttggatcgcg aaagagtcct ctctttttta atttttatga gaaacgtttt
    47821 gaaattgcga accgagaaaa aatttcagaa ttagaagcat taacgaaata tggtatttat
    47881 aatcgatatg agttacggtt agccaatgag aaagcaacac aagcagtaga agcatttatt
    47941 ttaggtgatt caccgaaacg tttaggagag attggcgttg gtttaattaa tgcaaaactt
    48001 attgtttggg atagactgga aagtgatgca cggattgttg ataaacgatg ggcaaaatta
    48061 tttcgttcga tggaagaatt ggattttaca gtaaagccag aaaaattctc attagatcgt
    48121 tcagaacgct ggtttttgaa acaagtagct ccgacattaa agttgttaca agaagcagat
    48181 cgtattttag gaagagactt tgtacaagaa gggattgata atgccgaact aaatgacgag
    48241 caacaaaact atctagaatg gatgggaacg tcaggaggga aaattgcatg aatgcatata
    48301 cgacaacaga atggctgcgc ctgttggatt taaaggctgc aatcgaagca ttaaatgaaa
    48361 agatggttga tttgtcttat tttcgttttc gagttcctta tattgaacaa gcggtaaaag
    48421 ctggacgtta tcaggaaaaa gagaactggc aagaaattgc acgcttatta gaagttcgta
    48481 agggatacga acaagaactc gaagagttgg aatttagccg aaggaaagga aggctagaat
    48541 tcattcgctt ttatcgattt tcattgccaa tacctgcgat tttagcggtt aaaaaaggct
    48601 gcgataaaat gaagatatat gaaaactgtg tggcagcact tagcagcgaa aaacctctta
    48661 tggaagaaat ttcattggtg actgtcactt gggaaatgag tcgccagcca acagaagaac
    48721 agacatatct ttctttagaa gagattgaga tagaattaga ggaaattggt cgctatgcca
    48781 cttgttctac ctattgtgga tcggtcatta gtattgctgg tgtgattgtc taaatgaaaa
    48841 aaattatgat ttatgttggt gcgtattggt cacgtgatcc aacagtatta gaaaatccag
    48901 aggcggtaca ctattgcttg cgccaattat tctatctgta taaagaacga ttagaatcgt
    48961 tgattcgtca gttaccatat actgacagac gtttagatga acttcttcta aggtatccag
    49021 caatgtataa acgacgaaaa aatagattgt taccagaaga gtatcccatt gaaaaacatg
    49081 aacttgaagg gcgatttgtg gcttatttct atgatgatgt tcgtatgcgc ttagtagaac
    49141 aacgaatgga aatagaaaat gatcgctact attttatcaa ttattgtaag aagcgtaagt
    49201 atcaagtaac tgatgatttt tatgattgta ttttacaaga tggggaagtg attctatcca
    49261 aaattgctcc aagttttcgt gaacttgtgc cgattgattt tttgaaaaaa tgtcatttgc
    49321 ggattcttcc gtaaaaaaga gctataaaag gaaagaaaag tagaagccaa atcggtttct
    49381 gcttttttat tttttttagg aaggagaaga tggtatgtca ctggaatttt taggagagcc
    49441 aattgaaaag gaagagttcg tttcgcatta tatggatttg tttattcagg tgataaaaaa
    49501 ctttgtgcga ttgatgaatt ttatccacgc gaacaagact atctagccgc tgaacgaata
    49561 aaaatcagtt tattatggga aaaattcaca gatgaaaata accgaccgcc agattatcgc
    49621 tatttatcag attgtttaac gaataatgtg ctttctaaag aatacctatt tcacaaacgt
    49681 gagaaaaatc aaatgtcggt tgaacatttt tgtgattgtt atattgcatt attgcgtagt
    49741 caacagttat taacagtaga acgatatggt gcagagtttg gttatgtcat gcaagctgag
    49801 cgtcatagag ctacaagatg ctatgaaaaa gaagatcaag tgattgatgg ctatgaagaa
    49861 ttatgtattc gaaacaatcg ctatttgcaa caacgtttat atgaacaatt gatgatggat
    49921 ttttcaaatc tctatagagg gtatgaatgt tcgatgattc aagaaaggta gaagaaaagg
    49981 gggaattttt atgtggcgat tattattttt ggtggtatgt ggtatggcat tgttctatgt
    50041 gtatcgtgtc tatctctatc cacgaatgga acgatggaca agttggtata gagatccttt
    50101 aaaacaacga aaaggattga agcggtatcg ttatgaatta cttgttgaat tttcggatgg
    50161 gcgacaagca cagtattatt tttttgcaaa ctatccaaaa tacaacacca gtgaactatt
    50221 gattcaatta ttcaaaaata atccatatgt tgggttagaa gaaaacggac gggagttctt
    50281 ttatcgttca gataatatta gtgcggcgtc tatgaacaaa acacgaattg cataaaaaat
    50341 tggaggaata atcatgaaaa aaacaacatt agcagtcgta acaacactag gaatttcatt
    50401 attttcgttt caattaggga gttcattggt ttatgcagat gaaactgtag aaacacctaa
    50461 gtcagaaatt gtagaaacat tgccagggga aggattacca gatgttgaga cagagctaaa
    50521 tggaggaaca acggaaaaac cagcagaacc tgaacaattg cagtcagaga aacctgaaaa
    50581 agagccagtg acaccagaac cagagtttcc tgaatcaact ttaccgactg attcgatcga
    50641 agaagaaggt gtgcatagtt caacaacaac ggattcaagt atagtcacaa ctccggaaca
    50701 atcaggaaca accagttctt ctgaaaagaa tactgattcg tcaagtacgg aacagccaac
    50761 gatgtcgtct accagtgaag aacaaaaaga agaaccgaaa caacctgttg aaaaatcaga
    50821 agcaattcca aaagaaaata aacctattgt tccagcaaaa gaagtcacag tatcggtaac
    50881 cccaagcggt gaaattacga cgaatacttc tcaaggaatg agtgtaccga ttgtcactag
    50941 taatgtagag gaattgaccc atattccaac accaaccacc ccattaaaag tagcaggtga
    51001 tcaaacaatt gttggtgtaa aagatggtat tccattaatc caagatagtg aagggaattt
    51061 ggtaaaggat ttgtcgattc ctgtaaagaa acttccaagt ggcaatattg aaatcaaaac
    51121 agcagatgga aaaacaaaag tcttaccaaa gactggtgaa gaaatacatg ttgcactttc
    51181 tgttctagga ggagtgttga caagtattgc tggatttgtt ggatataaaa gaaagagaaa
    51241 aggaatagaa aatgagggta attgaatgga ctatttttca gaaaaacaag cttatcgttg
    51301 tcaatcgtac gtactagtaa aaacatacta gaaggagtag cttatgaaaa aacgacaact
    51361 acggcggaaa gaaaaagttt atacagagaa agtaccgaag caaaagaaaa tccattcaaa
    51421 aaaagcagtg aaatggattt ggtgtttggt tttatttctt tgtttttcag ggggcttggc
    51481 attttttcaa tccatgaaaa cgaaggttca agtgtcgttt ttgaaagatg aaatccaaca
    51541 attacaaaag aaagaagtac aaagtcggtc agaattaacg tacacacctg aaattgaatc
    51601 ctttatgaat cgttttgttg ctttgtatat gaatacattc gataatcatg aacaagaaca
    51661 acaacgtcaa gaaacattaa aaaaagagtt ttatgcacaa aactttattc aagaaccagg
    51721 aagtgtttca ggtgttcgaa aattaactgc tgcttcgttt attggattaa aagaaattga
    51781 aaccattcca actgctagtt ataaagtaac ttatgatatt cgctcgatga atccagagag
    51841 taagaaagaa acaagtgtga caatcacaca agtactcaat cttccctttt ctatttcaaa
    51901 caagggctgt cgtattattg cttatcctta ttttacggca ctaccaaaga atacagaaaa
    51961 acaacgaatg attacttatg atgacacatt atacgaaacg gttgatgcgt cggaagcgaa
    52021 agatgttgaa ggatttgtgg aagatttctt agataagtat gcgaaaaaca atgcaaaaga
    52081 tatgtcatac atgatgaaag aacctgaagg actcaatcaa acagctaaag ttgatcaagt
    52141 agagatgaaa gtattgcgtt ataaagaaac gaaaaaacct tttattgtaa aagcaacggt
    52201 cgtctttcga gatggtgata cgtcactttt acaaaaagaa caaatgacga ttgtcattac
    52261 gaaaaaaaca aatcagtatt atgttgaaaa attaacacat aattggttgg ataggaaagg
    52321 agaagaacgg aaatgacact gaagggaatt gtagatttta tattaacgca aggtggctat
    52381 tttttaataa tggtggttgt ttatctggga ttgcgaaatt ttatgaaatc aaaagtagga
    52441 aatgcattaa tatctatcgc agcaggtgcc gcagtttatt tcttcttaaa tgatccaaca
    52501 cgtgtatttg atgcgtttgg taaaatcatt gaaaaattat ttatggatta ggaggatagt
    52561 gaagtggatt gggaacctta tgattattcg attgtttatg cggaacctta tcgcgtacaa
    52621 cagattacaa gcaattggcg tcttccattt ccagttgcat tagtcaatgc agcaactttt
    52681 ttgttggtcg taatctttga atatttattt ttacgttcat ttattggttg ggtcatttct
    52741 ttttttccac cagcaggagt tgttttttat ataggactcc cctatttatg tgtgttagca
    52801 ctggcaaaac taaaacctga tggaaaaaaa gtgcattttt atttatggga ttggttttgt
    52861 tatatgattc gtatgatgaa tggtgattgt gtgtattata actgggacaa ggtatcaaac
    52921 aatgcattgg aagaattact ttttgtgaat aagtatgttt atttagagga ttagaaaaag
    52981 ggacaatcaa ctggttgttc cttttttatt gggaggtgaa attcattggt gcttgtaaaa
    53041 gaaccagtaa aagcattcaa gaaaaattgt ttgttaaatg gagaggattg tatctgggcc
    53101 tactatcatg tttttccacg aacaatttca aaaaacacac caaaaaagac acaggaaata
    53161 aaagaaatga tggctcgttt actgttgctt ttagcagatt atgaagatgt ggagttggtg
    53221 atgtatccac gaacaatgaa tttacggacg aagtttgaca cattaggaaa gggattttct
    53281 cccgatagtc aagcaattgg tgcgtattat gcaaaagaaa cagtgacatt gttggaacga
    53341 gaaggtcaag tctatcgtcc ttcatttatt ttaggaatca aagtcaaaga aaaagcaacg
    53401 gtggagagtt ttcatgatgt aataaatcta atctggttag atttagcagg aagtttagca
    53461 cgcttatttg gattagaagt agatgatttg gaaactttgt tttctgaaag tgatccttat
    53521 gtaaaagaag tgacacaatt actttatagc ttttctattc gtcccttagt aaaaaaagag
    53581 ctgttgtatc tcaatcgtct gcaatatatt cgaggaatgg ctcacaaagt agaagaacaa
    53641 aataattggg aagtactgaa agacaatcaa ggaacgtatg tacgtatacc gaaggaacat
    53701 aaaggccttc ttcaattaga agatgagtta gggagtagtt atttagcatt gttgcctatt
    53761 ggggaaacac ctgaaaatat gagaaattgt gatctatttc aatttgctca ggatctttct
    53821 ttcccagcag aattgcgaat gaaaattcac tatccacgct tgaaaggaca gggaggtatt
    53881 ggtttaaaac tgaattggct atcggaacgg ttcaaaacag agcataagga attggttcaa
    53941 aatgatgaag aacctagtga acgcttatta actgttcgag gattagtaaa acatttaaag
    54001 caacgagtag ataatgggca acctgtcgtc gattggatcg catgttttat tgtatacggt
    54061 caaacggttg aagaagttcg tcaaaatagt gcaacggtta ttcaacaact tcatacgatt
    54121 atccatatcc accgtgcaaa aaatgatcaa cgagaattgt tctataaatg tttattaggg
    54181 caaccattgg gacggcagtt acattggaag caccgaacaa ctgtagaagc agttggcgaa
    54241 tggttgtttt cagcagtatc agaattaggt atggaatccg gctggtatat tggtcggcaa
    54301 gatacattag gggatggtgg ttctcgagga gaaaaaacac cattagaaga attaatttat
    54361 gcgtcaaatc gttttgtctt tttgaatctt ctagccattg cggaaggaat caaaggtgcg
    54421 ttgtttgatg cgcctcatgt agcagtaact ggaaaaaccg gtaaagggaa gaccttttta
    54481 gttggcttgc tctttttgta tagttcgttt atgaatgtac aaagtttatt tgtggatccc
    54541 aaaggagaaa agcgcaaatg gtttacgaaa ctcattcaag acccgtatta tcagaaacat
    54601 taccctttgt ttgtagaaca tttgcaaaaa atgagttatg tcacgttaga tgctacaaat
    54661 ccccgtaatt atggtgtatt agatccaatt gtttgtttat cccgagtaga tgcgaaacaa
    54721 gtagcccagg atatgattga tgagttaagt ccgttaggta ccaatcacct tttaaaaggg
    54781 gcggtattaa agggaatcca agaagtttta gttcaaaaag aagccggaca acaagttggc
    54841 ttgatgcatg tggtcgacta tttagaacaa atggaacaaa aagaggttcg agaatatggt
    54901 gagtttttac gcttgacggt agaagattcg attttacgtt taggattttc ttatggagaa
    54961 aacccgggat tagactttaa tgccaaaaca acgattttag agatccaaga tttgaaatta
    55021 ccaaatgaca atgtccgacc agaactatat acagatgcgg atcgaaagtc tttgtgtttg
    55081 atgattagtt taggtcgttt ttgtgaaatg tttggaaaac gagatagtag taagaaaaca
    55141 gcgatttatt ttacagaagc atgggtattt aataattcga acgcaggacg tagtattatt
    55201 gcagcaatgg cacgtgtggg gcgttcccag atgaatcaat tagtgttaga tacacaattt
    55261 attggtgact taggttcaga taaagaaaaa gggaattttg gtgtcgtctt tgcttttgat
    55321 gaagacagtg agcgagaaaa gattttgaat catttacgtt tggaagtaaa tgaagcgaac
    55381 ttagctgaaa tgagccacat gattaaagga cagtgttgga tgttagatcc ctatggacgt
    55441 gtaggaaaac tcaatgtaca cagtttgttt gaagaaatga ccgctgcatt acaaacaacg
    55501 gaaaaaacaa gtcactctca agtagaagaa caggatacgt taatcatgca atagaaatga
    55561 ggtgaaacaa tggcttatat aaagcataag gaaaagaaat ggtgcgtttt gtttttttta
    55621 acgattggat tcggactgct ttttgttggt ggattggtgt atgcggcaga aaagcaggtg
    55681 gatcaatcga tctatgagat tgggaaattt catattgata ccttttcttc ttatttatct
    55741 gatagtgaat tttcattagg caaaatgtta tttggggcaa atggcacaga ttttctaaaa
    55801 atgatgacga atatgttctt tagtttaagt aaattgattt ggcaaggcct tgattttgtc
    55861 attgagaagc tctatgaagg agctgcaatg aacgagttaa ttcaccagtt tttcactttt
    55921 tcggatacta tttattcaaa aatatacgat cctattggtg cggggattgt cgtcttgtat
    55981 gtgttatatc tatttactct tcgtatattt agagggaaac attacgcaaa aatggtgttt
    56041 attcgatttc tcttcgttgt cattttttca atggtttggt ttggctatgg tagtaacgta
    56101 cctagtaaag gagaagcttt taccaaaggg ttaaatgatt tgtccgtgga ggtagaaggc
    56161 tttatttttt ctgctacaaa tagtctagat gaattggcga caaaagatgt caatggtgtt
    56221 ttagtgccaa cgacacaaaa ggaagccatt caaaaagtgc gccaacttta ttataaagcg
    56281 gctgttgttg atccgtatat attgttaaat tatgggacaa caaatttaga agaattgtat
    56341 caagcagaga ttgaaccaca agaatttcta gccaaggaag cgtcaagtga agaattatct
    56401 gatattgaga caaaagtgac cactgctgca ggagaagata gagggaaaaa gaaggcttat
    56461 cgtgtctata ttcaaccaac taaagctgtt tataaagcag tcgttggttc tatggtgcca
    56521 ttgcttaatc taagtattgg attaccactt gtagtgattg gcatgattcg ttttatcttt
    56581 caaatgttgg tgttgtgttt attacttgtg attccttttt cattactcgc ttcttttgtt
    56641 ccagtatttg attatatgct catgaatacc ttaaagtcct ttttgggctt gatgtttcaa
    56701 aaatcttttt acagtgtgat tattttggtg gcatttttga tctataacat agtagacgat
    56761 ttaattccaa tgaatactgt ttttggtttt ttaggaaata tgtttgtcaa agcactccta
    56821 tcaattttac tcattgtaaa acggaaagat ttattaaaga aactagggtt aggagctgca
    56881 gatcaaacta taaacaacac gaaaaacata gcaagtcatt ctttgtctca aatgaaacaa
    56941 ctgcctggtc aactatcaac tgctggacaa aatttggcaa tcaaaggtgc aggattagca
    57001 gggaatgtgt atcgagcaac gaatccactc gcttctgcag cgatacatgg atttcaaaag
    57061 tttcaacaaa aacgtacacc acaagaatcg gtaaaaggga ggcaagtggt taatcccttt
    57121 gaactaggtg ggcgttcaaa tcaagccttg ccttatagtg gtcgtcatgt tcgttatcat
    57181 ggggtagagc ctgaaatgtc ttctaccaaa tcgaaaacag agtggaattc tggtctttca
    57241 aatggtttga ttccaaattc gtccatgaat tcagtaagac caaaacaaca ggtacaatca
    57301 ttggcacaat tgagtaagca acaagggaat aatcaaagaa aaccattgtt aaataaaggt
    57361 atcgcttttg ttggtcaatc gattccgttg ccttctggaa aagtacccgt gccacaatta
    57421 acagctgctg gttctctttc acaacagatt cgccaagtga aaaatcaggc gaaaaatggg
    57481 agttatccag caatgcagca gttattgaac caatactctg ttcctaattc gtcggtagcc
    57541 actctatcag gaaatcgttc tgtgcaacaa ccggggcaac gttcgtctga aagtaaaccg
    57601 tcgatttccg caccaaaatc gttacaacct gcattttctt ctttagataa gccaacagag
    57661 caatcggaaa aaatagaaaa ggatcctaaa acactgccga ttttggacgc aaaaccagga
    57721 actgatttta ctttaccaaa aggacgaaca gtccaaaaac cattatcgaa gaaaggatga
    57781 ggaagaaatg aagaaagggt gtctaggttg tcttcttttc tttattcttc ccttttttct
    57841 attgccattc tttttttcag gagaactttc tgagcatgga ggagaagcta caggtctagc
    57901 tgtcgctttt aatggggaat ataccgaagg attaccgatt ttttcagaga ttaaaggacg
    57961 tggtccaatc acagatgaac atgcgcaagc agcagttggt gcagctgtaa agtatcagtt
    58021 attaccgagt gtgattcttt ctcaattagg ctatgaatca gcttatggac agtcacaatc
    58081 tggaagaaac gatacgaact tttttgggat tacctggttt cctggttgtc cctacccaca
    58141 aggttctgca cgtggaattg gtggttctga aggtggtttc tatatgaagt ttccgaactt
    58201 aaaagcttgt tatgactatt atggctttat ggtggcgagc caaagcaact ttaatcaatg
    58261 tgtgggcaat aaagatcctg gaagttgttt attgattcta ggtcgtggag gctatgcggc
    58321 agcaggtatt accgaaggga gtccgtatta tcgaggagca atgagtatca taacgtccta
    58381 taacttaacg gaatatgata cttttgcgat caagcattgg aatagcctac caacaaaaaa
    58441 aacttcgctt attgcaggaa aaagtaaagg caatgcgaag gttttggatc ggatcattgg
    58501 acgatccgta aataatgggg aatgttatgg tggtacggca tggtatgtat ctcaattagg
    58561 tggcccacag ttaatgggtt ctggacacat gtttgcgatg ttaatagggg aagattataa
    58621 ctggacaacc tatggttggc aagtaatcat gaacccaaaa ccaagtgatt tacaagcagg
    58681 agatgtggtg aattggcaag caggtggtgc attgtctcct ggaatctatg gtcacaccgg
    58741 aattataaca gatgtttcat atggcggaca acatttctct agtgtggaac agaatgccga
    58801 gcaaggacgg attatggctc ggtatcaacg cacgtacaat caaacaaaaa ttcgtagtct
    58861 ggttcgtaag gtgaaataag aaaggcagtg actagaaacg tgaaaaaagg gataatttta
    58921 gtatttagtt ggttatgttt gataggtctt actggttgtc attggttttc ttctgaaaaa
    58981 caaaaaacac aattgagaac gacagaagaa acaatcgaag cagtaaaaga aacaagtgga
    59041 actcttcaaa gtactgaaag taacagtcaa attggttcat tagaagacga gacagcgcaa
    59101 caaatgcaaa tagatgaaat gacaaaaaat ttcttaaaag attttgctag aaaatggagt
    59161 aatttcacag atgtttataa acgcaatcaa gctgtgaggg actatatgac agatcgttgt
    59221 attaaagaca atagtattga tgtagacccg catgtacaaa tgaaaacaac tgggaaactt
    59281 taccaggtga cacaagatat agagaatccg gatcaatatt tgttatttgg agaagaaaca
    59341 atgaataagg ttactcaatt tgtattactt caattgaaag tagatcaaga agctcaaaag
    59401 attgatgcga tcaagattta ttatgtacgg agtgcgtatt aaaataaaaa atgaacactc
    59461 atatttatta tacaccgaag aaatagaaaa aaacagactg tttttttaag aaaaactatg
    59521 tcattcttta cttgaaaaaa gttgttatag gacagaaagg ataaaacaaa aacggaaaaa
    59581 atatgtctta tgaataacta taagaactat tctatttttt aggagatgga tacgagatga
    59641 aaaaactaat tggctatagt gtgatgtgta ttggatttgt tggtgtggga ttgtttgtat
    59701 cgactgaatc tgcttctgcg gctgaatggt ataatcctat ctggtataat ccgaacattg
    59761 aatgtcaaaa aggctatccg tatggaccga caaatctgaa aaatggaaaa tgtagagtaa
    59821 attggaatgg cgttgtagat agtgtaatta ctggttcaat ggactctgct ctacatggct
    59881 ttacgggtca ctagaaggag gaagaaaatg tctgaagaga aaaaagaaat cgttgaatca
    59941 ttagtagctt taaaagattc tctttctaag attgaatatg ttgataggca agaaattcaa
    60001 aaactaattg atgatactat tattgaaatc caagatgcgc gttgtgaagg tataaaaatt
    60061 tctgtagctt tatctaaagt aatagaaaaa atgaaccgtt cattagcgtt taatggattg
    60121 aagctagata gacaaactag tctgatatgg gatcatttaa aagacttgta tgacaagtct
    60181 aaaagaagtg aacggactgc tgtatcaatc ttaaaaggcc tttggggaat gaattc
//
LOCUS       DAYYBG010000006        58825 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000006 DAYYBG010000000
VERSION     DAYYBG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.006"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..535
                     /locus_tag="ACHAUN_000364"
     CDS             17..535
                     /locus_tag="ACHAUN_000364"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8459098.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            663..1346
                     /locus_tag="ACHAUN_000365"
     CDS             663..1346
                     /locus_tag="ACHAUN_000365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8459099.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1655..1728
                     /locus_tag="ACHAUN_000366"
     tRNA            1655..1728
                     /locus_tag="ACHAUN_000366"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Arg,seq:tct)
     gene            1887..2246
                     /locus_tag="ACHAUN_000367"
     CDS             1887..2246
                     /locus_tag="ACHAUN_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8459100.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2369..4000
                     /locus_tag="ACHAUN_000368"
     CDS             2369..4000
                     /locus_tag="ACHAUN_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8459101.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4062..4703)
                     /locus_tag="ACHAUN_000369"
     CDS             complement(4062..4703)
                     /locus_tag="ACHAUN_000369"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8459102.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4889..4965
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4985..5293
                     /gene="rplU"
                     /locus_tag="ACHAUN_000370"
     CDS             4985..5293
                     /gene="rplU"
                     /locus_tag="ACHAUN_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8459103.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5320..5664
                     /locus_tag="ACHAUN_000371"
     CDS             5320..5664
                     /locus_tag="ACHAUN_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8459104.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5677..5970
                     /gene="rpmA"
                     /locus_tag="ACHAUN_000372"
     CDS             5677..5970
                     /gene="rpmA"
                     /locus_tag="ACHAUN_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8459105.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6404..9211
                     /locus_tag="ACHAUN_000373"
     CDS             6404..9211
                     /locus_tag="ACHAUN_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8459106.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9402..9893
                     /locus_tag="ACHAUN_000374"
     CDS             9402..9893
                     /locus_tag="ACHAUN_000374"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8459107.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9996..10985
                     /locus_tag="ACHAUN_000375"
     CDS             9996..10985
                     /locus_tag="ACHAUN_000375"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8459108.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11018..11821
                     /locus_tag="ACHAUN_000376"
     CDS             11018..11821
                     /locus_tag="ACHAUN_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8459109.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11842..12756
                     /locus_tag="ACHAUN_000377"
     CDS             11842..12756
                     /locus_tag="ACHAUN_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8459110.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12839..13213
                     /locus_tag="ACHAUN_000378"
     CDS             12839..13213
                     /locus_tag="ACHAUN_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8459111.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13352..13597
                     /locus_tag="ACHAUN_000379"
     CDS             13352..13597
                     /locus_tag="ACHAUN_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8459112.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13769..14833
                     /locus_tag="ACHAUN_000380"
     CDS             13769..14833
                     /locus_tag="ACHAUN_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8459113.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15079..15504
                     /locus_tag="ACHAUN_000381"
     CDS             15079..15504
                     /locus_tag="ACHAUN_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8459114.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15497..15946
                     /gene="nusB"
                     /locus_tag="ACHAUN_000382"
     CDS             15497..15946
                     /gene="nusB"
                     /locus_tag="ACHAUN_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8459115.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16043..16897
                     /locus_tag="ACHAUN_000383"
     CDS             16043..16897
                     /locus_tag="ACHAUN_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8459116.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16898..18235
                     /gene="xseA"
                     /locus_tag="ACHAUN_000384"
     CDS             16898..18235
                     /gene="xseA"
                     /locus_tag="ACHAUN_000384"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8459117.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18240..18464
                     /locus_tag="ACHAUN_000385"
     CDS             18240..18464
                     /locus_tag="ACHAUN_000385"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8459118.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18466..19356
                     /locus_tag="ACHAUN_000386"
     CDS             18466..19356
                     /locus_tag="ACHAUN_000386"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8459119.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19365..20183
                     /locus_tag="ACHAUN_000387"
     CDS             19365..20183
                     /locus_tag="ACHAUN_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8459120.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20290..20739
                     /locus_tag="ACHAUN_000388"
     CDS             20290..20739
                     /locus_tag="ACHAUN_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8459121.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20757..22439
                     /gene="recN"
                     /locus_tag="ACHAUN_000389"
     CDS             20757..22439
                     /gene="recN"
                     /locus_tag="ACHAUN_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8459122.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22494..22610)
                     /locus_tag="ACHAUN_000390"
     CDS             complement(22494..22610)
                     /locus_tag="ACHAUN_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8459123.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22683..23639)
                     /locus_tag="ACHAUN_000391"
     CDS             complement(22683..23639)
                     /locus_tag="ACHAUN_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8459124.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23810..24178
                     /locus_tag="ACHAUN_000392"
     CDS             23810..24178
                     /locus_tag="ACHAUN_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8459125.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24348..24779
                     /gene="mraZ"
                     /locus_tag="ACHAUN_000393"
     CDS             24348..24779
                     /gene="mraZ"
                     /locus_tag="ACHAUN_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8459126.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24800..25759
                     /gene="rsmH"
                     /locus_tag="ACHAUN_000394"
     CDS             24800..25759
                     /gene="rsmH"
                     /locus_tag="ACHAUN_000394"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8459127.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25764..26165
                     /gene="ftsL"
                     /locus_tag="ACHAUN_000395"
     CDS             25764..26165
                     /gene="ftsL"
                     /locus_tag="ACHAUN_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8459128.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26166..28358
                     /locus_tag="ACHAUN_000396"
     CDS             26166..28358
                     /locus_tag="ACHAUN_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8459129.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28384..29346
                     /gene="mraY"
                     /locus_tag="ACHAUN_000397"
     CDS             28384..29346
                     /gene="mraY"
                     /locus_tag="ACHAUN_000397"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8459130.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29360..30721
                     /gene="murD"
                     /locus_tag="ACHAUN_000398"
     CDS             29360..30721
                     /gene="murD"
                     /locus_tag="ACHAUN_000398"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8459131.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30747..31835
                     /gene="murG"
                     /locus_tag="ACHAUN_000399"
     CDS             30747..31835
                     /gene="murG"
                     /locus_tag="ACHAUN_000399"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8459132.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31850..33070
                     /locus_tag="ACHAUN_000400"
     CDS             31850..33070
                     /locus_tag="ACHAUN_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8459133.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33226..34551
                     /gene="ftsA"
                     /locus_tag="ACHAUN_000401"
     CDS             33226..34551
                     /gene="ftsA"
                     /locus_tag="ACHAUN_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8459134.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34574..35815
                     /gene="ftsZ"
                     /locus_tag="ACHAUN_000402"
     CDS             34574..35815
                     /gene="ftsZ"
                     /locus_tag="ACHAUN_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8459135.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35832..36509
                     /locus_tag="ACHAUN_000403"
     CDS             35832..36509
                     /locus_tag="ACHAUN_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8459136.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36523..37122
                     /locus_tag="ACHAUN_000404"
     CDS             36523..37122
                     /locus_tag="ACHAUN_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8459137.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37203..37424
                     /locus_tag="ACHAUN_000405"
     CDS             37203..37424
                     /locus_tag="ACHAUN_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8459138.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37473..38255
                     /locus_tag="ACHAUN_000406"
     CDS             37473..38255
                     /locus_tag="ACHAUN_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8459139.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38276..38983
                     /locus_tag="ACHAUN_000407"
     CDS             38276..38983
                     /locus_tag="ACHAUN_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8459140.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39245..42043
                     /gene="ileS"
                     /locus_tag="ACHAUN_000408"
     CDS             39245..42043
                     /gene="ileS"
                     /locus_tag="ACHAUN_000408"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8459141.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42092..43618)
                     /gene="zwf"
                     /locus_tag="ACHAUN_000409"
     CDS             complement(42092..43618)
                     /gene="zwf"
                     /locus_tag="ACHAUN_000409"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8459142.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43633..44280)
                     /locus_tag="ACHAUN_000410"
     CDS             complement(43633..44280)
                     /locus_tag="ACHAUN_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8459143.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44464..44793)
                     /locus_tag="ACHAUN_000411"
     CDS             complement(44464..44793)
                     /locus_tag="ACHAUN_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8459144.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44970..45698
                     /gene="sfsA"
                     /locus_tag="ACHAUN_000412"
     CDS             44970..45698
                     /gene="sfsA"
                     /locus_tag="ACHAUN_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8459145.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45714..46727
                     /locus_tag="ACHAUN_000413"
     CDS             45714..46727
                     /locus_tag="ACHAUN_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8459146.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46727..48004
                     /locus_tag="ACHAUN_000414"
     CDS             46727..48004
                     /locus_tag="ACHAUN_000414"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8459147.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48067..50769
                     /locus_tag="ACHAUN_000415"
     CDS             48067..50769
                     /locus_tag="ACHAUN_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8459148.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50921..51238
                     /locus_tag="ACHAUN_000416"
     CDS             50921..51238
                     /locus_tag="ACHAUN_000416"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8459149.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51268..51588
                     /locus_tag="ACHAUN_000417"
     CDS             51268..51588
                     /locus_tag="ACHAUN_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8459150.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51696..53156
                     /locus_tag="ACHAUN_000418"
     CDS             51696..53156
                     /locus_tag="ACHAUN_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8459151.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53224..53445
                     /locus_tag="ACHAUN_000419"
     CDS             53224..53445
                     /locus_tag="ACHAUN_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459152.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53476..53658
                     /locus_tag="ACHAUN_000420"
     CDS             53476..53658
                     /locus_tag="ACHAUN_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8459153.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53658..54071
                     /locus_tag="ACHAUN_000421"
     CDS             53658..54071
                     /locus_tag="ACHAUN_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8459154.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54194..55375
                     /locus_tag="ACHAUN_000422"
     CDS             54194..55375
                     /locus_tag="ACHAUN_000422"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8459155.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55446..55610)
                     /locus_tag="ACHAUN_000423"
     CDS             complement(55446..55610)
                     /locus_tag="ACHAUN_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459156.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55764..55837
                     /locus_tag="ACHAUN_000424"
     tRNA            55764..55837
                     /locus_tag="ACHAUN_000424"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55798..55800,aa:Arg,seq:cct)
     gene            complement(55906..57045)
                     /locus_tag="ACHAUN_000425"
     CDS             complement(55906..57045)
                     /locus_tag="ACHAUN_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8459157.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57344..57979)
                     /locus_tag="ACHAUN_000426"
     CDS             complement(57344..57979)
                     /locus_tag="ACHAUN_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459158.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58092..58727)
                     /locus_tag="ACHAUN_000427"
     CDS             complement(58092..58727)
                     /locus_tag="ACHAUN_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8459159.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT        19319 a         9938 c        12490 g        17078 t
ORIGIN      
        1 aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt
       61 gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac
      121 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
      181 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
      241 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
      301 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
      361 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
      421 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
      481 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
      541 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
      601 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
      661 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
      721 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
      781 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
      841 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
      901 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
      961 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     1021 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     1081 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     1141 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     1201 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     1261 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     1321 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     1381 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     1441 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     1501 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     1561 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     1621 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     1681 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     1741 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     1801 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     1861 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     1921 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     1981 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     2041 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     2101 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     2161 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     2221 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     2281 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     2341 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     2401 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     2461 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     2521 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     2581 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     2641 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     2701 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     2761 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     2821 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     2881 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     2941 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     3001 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     3061 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     3121 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     3181 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     3241 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     3301 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     3361 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     3421 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     3481 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     3541 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     3601 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     3661 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     3721 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     3781 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     3841 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     3901 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     3961 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     4021 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     4081 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     4141 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     4201 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     4261 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     4321 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     4381 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     4441 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     4501 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     4561 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     4621 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     4681 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     4741 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     4801 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     4861 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     4921 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     4981 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     5041 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     5101 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     5161 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     5221 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     5281 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     5341 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     5401 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     5461 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     5521 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     5581 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     5641 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     5701 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     5761 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     5821 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     5881 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     5941 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     6001 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     6061 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     6121 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     6181 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     6241 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     6301 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     6361 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     6421 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     6481 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     6541 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     6601 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     6661 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     6721 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     6781 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     6841 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     6901 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     6961 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     7021 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     7081 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     7141 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     7201 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     7261 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     7321 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     7381 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     7441 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     7501 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     7561 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     7621 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     7681 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     7741 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     7801 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     7861 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     7921 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     7981 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     8041 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     8101 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     8161 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     8221 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     8281 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     8341 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     8401 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     8461 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     8521 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     8581 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     8641 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     8701 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     8761 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     8821 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     8881 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     8941 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     9001 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     9061 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     9121 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     9181 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     9241 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     9301 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     9361 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     9421 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     9481 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     9541 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     9601 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     9661 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     9721 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     9781 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     9841 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     9901 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     9961 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    10021 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    10081 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    10141 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    10201 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    10261 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    10321 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    10381 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    10441 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    10501 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    10561 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    10621 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    10681 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    10741 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    10801 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    10861 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    10921 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    10981 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    11041 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    11101 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    11161 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    11221 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    11281 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    11341 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    11401 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    11461 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    11521 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    11581 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    11641 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    11701 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    11761 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    11821 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    11881 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    11941 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    12001 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    12061 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    12121 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    12181 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    12241 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    12301 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    12361 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    12421 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
    12481 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    12541 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    12601 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    12661 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    12721 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    12781 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    12841 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    12901 gacagattac gggcaaatca tgataggtaa taatggtttt gagttttacc atgcgcgaga
    12961 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    13021 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    13081 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    13141 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    13201 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    13261 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    13321 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    13381 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    13441 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    13501 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    13561 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    13621 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    13681 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    13741 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    13801 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    13861 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    13921 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    13981 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    14041 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    14101 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    14161 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    14221 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    14281 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    14341 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    14401 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    14461 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    14521 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    14581 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    14641 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    14701 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    14761 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    14821 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    14881 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    14941 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    15001 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    15061 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    15121 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    15181 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    15241 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    15301 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    15361 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    15421 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    15481 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    15541 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    15601 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    15661 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    15721 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    15781 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    15841 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    15901 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    15961 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    16021 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    16081 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    16141 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    16201 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    16261 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    16321 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    16381 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    16441 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    16501 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    16561 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    16621 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    16681 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    16741 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    16801 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    16861 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    16921 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    16981 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    17041 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    17101 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    17161 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    17221 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    17281 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    17341 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    17401 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    17461 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    17521 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    17581 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    17641 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    17701 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    17761 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    17821 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    17881 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    17941 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    18001 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    18061 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    18121 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    18181 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    18241 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    18301 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    18361 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    18421 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    18481 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    18541 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    18601 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    18661 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    18721 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    18781 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    18841 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    18901 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    18961 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    19021 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    19081 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    19141 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    19201 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    19261 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    19321 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    19381 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    19441 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    19501 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    19561 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    19621 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    19681 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    19741 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    19801 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    19861 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    19921 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    19981 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    20041 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    20101 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    20161 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    20221 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    20281 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    20341 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    20401 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    20461 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    20521 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    20581 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    20641 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    20701 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    20761 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    20821 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    20881 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    20941 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    21001 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    21061 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    21121 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    21181 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    21241 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    21301 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    21361 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    21421 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    21481 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    21541 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    21601 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    21661 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    21721 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    21781 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    21841 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    21901 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    21961 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    22021 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    22081 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    22141 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    22201 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    22261 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    22321 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    22381 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    22441 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    22501 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    22561 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    22621 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    22681 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    22741 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    22801 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    22861 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    22921 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    22981 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    23041 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    23101 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    23161 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    23221 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    23281 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    23341 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    23401 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    23461 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    23521 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    23581 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    23641 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    23701 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    23761 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    23821 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    23881 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    23941 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    24001 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    24061 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    24121 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    24181 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    24241 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    24301 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    24361 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    24421 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    24481 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    24541 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    24601 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    24661 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    24721 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    24781 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    24841 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    24901 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    24961 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    25021 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    25081 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    25141 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    25201 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    25261 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    25321 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    25381 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    25441 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    25501 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    25561 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    25621 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    25681 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    25741 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    25801 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    25861 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    25921 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    25981 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    26041 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    26101 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    26161 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    26221 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    26281 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    26341 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    26401 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    26461 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    26521 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    26581 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    26641 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    26701 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    26761 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    26821 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    26881 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    26941 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    27001 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    27061 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    27121 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    27181 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    27241 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    27301 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    27361 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    27421 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    27481 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    27541 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    27601 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    27661 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    27721 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    27781 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    27841 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    27901 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    27961 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    28021 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    28081 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    28141 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    28201 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    28261 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    28321 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    28381 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    28441 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    28501 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    28561 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    28621 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    28681 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    28741 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    28801 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    28861 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    28921 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    28981 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    29041 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    29101 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    29161 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    29221 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    29281 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    29341 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    29401 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    29461 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    29521 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    29581 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    29641 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    29701 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    29761 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    29821 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    29881 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    29941 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    30001 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    30061 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    30121 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    30181 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    30241 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    30301 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    30361 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    30421 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    30481 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    30541 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    30601 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    30661 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    30721 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    30781 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    30841 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    30901 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    30961 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    31021 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    31081 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    31141 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    31201 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    31261 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    31321 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    31381 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    31441 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    31501 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    31561 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    31621 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    31681 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    31741 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    31801 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    31861 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    31921 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    31981 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    32041 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    32101 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    32161 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    32221 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    32281 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    32341 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    32401 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    32461 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    32521 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    32581 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    32641 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    32701 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    32761 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    32821 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    32881 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    32941 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    33001 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    33061 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    33121 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    33181 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    33241 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    33301 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    33361 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    33421 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    33481 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    33541 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    33601 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    33661 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    33721 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    33781 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    33841 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    33901 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    33961 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    34021 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    34081 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    34141 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    34201 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    34261 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    34321 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    34381 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    34441 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    34501 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    34561 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    34621 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    34681 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    34741 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    34801 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    34861 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    34921 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    34981 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    35041 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    35101 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    35161 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    35221 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    35281 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    35341 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    35401 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    35461 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    35521 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    35581 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    35641 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    35701 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    35761 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    35821 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    35881 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    35941 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    36001 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    36061 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    36121 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    36181 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    36241 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    36301 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    36361 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    36421 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    36481 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    36541 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    36601 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    36661 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    36721 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    36781 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    36841 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    36901 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    36961 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    37021 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    37081 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    37141 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    37201 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    37261 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    37321 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    37381 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    37441 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    37501 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    37561 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    37621 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    37681 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    37741 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    37801 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    37861 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    37921 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    37981 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    38041 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    38101 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    38161 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    38221 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    38281 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    38341 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    38401 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    38461 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    38521 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    38581 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    38641 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    38701 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    38761 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    38821 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    38881 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    38941 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    39001 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    39061 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    39121 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    39181 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    39241 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    39301 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    39361 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    39421 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    39481 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    39541 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    39601 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    39661 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    39721 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    39781 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    39841 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    39901 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    39961 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    40021 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    40081 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    40141 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    40201 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    40261 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    40321 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    40381 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    40441 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    40501 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    40561 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    40621 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    40681 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    40741 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    40801 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    40861 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    40921 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    40981 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    41041 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    41101 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    41161 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    41221 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    41281 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    41341 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    41401 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    41461 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    41521 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    41581 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    41641 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    41701 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    41761 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    41821 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    41881 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    41941 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    42001 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    42061 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    42121 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    42181 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    42241 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    42301 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    42361 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    42421 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    42481 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    42541 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    42601 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    42661 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    42721 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    42781 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    42841 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    42901 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    42961 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    43021 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    43081 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    43141 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    43201 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    43261 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    43321 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    43381 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    43441 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    43501 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    43561 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    43621 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    43681 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    43741 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    43801 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    43861 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    43921 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    43981 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    44041 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    44101 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    44161 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    44221 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    44281 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    44341 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    44401 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    44461 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    44521 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    44581 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    44641 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    44701 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    44761 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    44821 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    44881 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    44941 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    45001 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    45061 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    45121 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    45181 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    45241 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    45301 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    45361 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    45421 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    45481 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    45541 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    45601 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    45661 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    45721 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    45781 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    45841 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    45901 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    45961 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    46021 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    46081 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    46141 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    46201 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    46261 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    46321 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    46381 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    46441 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    46501 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    46561 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    46621 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    46681 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    46741 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    46801 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    46861 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    46921 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    46981 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    47041 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    47101 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    47161 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    47221 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    47281 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    47341 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    47401 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    47461 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    47521 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    47581 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    47641 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    47701 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    47761 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    47821 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    47881 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    47941 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    48001 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    48061 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    48121 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    48181 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    48241 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    48301 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    48361 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    48421 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    48481 gcagatgcgc catttgtttc tttcaattgt gcggattatg atcaaaatcc gcaactgctc
    48541 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    48601 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    48661 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    48721 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    48781 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    48841 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    48901 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    48961 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    49021 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    49081 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    49141 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    49201 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    49261 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    49321 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    49381 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    49441 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    49501 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    49561 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    49621 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    49681 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    49741 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    49801 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    49861 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    49921 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    49981 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    50041 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    50101 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    50161 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    50221 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    50281 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    50341 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    50401 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    50461 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    50521 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    50581 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    50641 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    50701 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    50761 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    50821 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    50881 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    50941 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    51001 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    51061 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    51121 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    51181 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    51241 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    51301 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    51361 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    51421 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    51481 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    51541 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    51601 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    51661 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    51721 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    51781 gtgatgcttt tatcggcata ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    51841 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    51901 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    51961 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    52021 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    52081 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    52141 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    52201 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    52261 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    52321 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    52381 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    52441 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    52501 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    52561 atggatcaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    52621 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    52681 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    52741 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    52801 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    52861 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    52921 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    52981 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    53041 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    53101 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    53161 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    53221 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    53281 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    53341 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    53401 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    53461 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    53521 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    53581 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    53641 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    53701 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    53761 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    53821 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    53881 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    53941 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    54001 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    54061 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    54121 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    54181 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    54241 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    54301 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    54361 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    54421 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    54481 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    54541 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    54601 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    54661 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    54721 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    54781 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    54841 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    54901 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    54961 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    55021 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    55081 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    55141 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    55201 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    55261 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    55321 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    55381 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    55441 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    55501 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    55561 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    55621 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    55681 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    55741 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    55801 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    55861 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    55921 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
    55981 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
    56041 gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg
    56101 caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac
    56161 ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt
    56221 gatttcaaaa ttctcgaata attctttttg attgatttta aactttttta agagatcaat
    56281 cgtatgttta tcaatggaaa ttttcctatt tgaagtttcg ttttttgttt ttccccattc
    56341 atttaattta taattccatg ttttattaat agtgatgatt tgatcttcga aatcgatatc
    56401 ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca gtggcaccag caactaacac
    56461 gagcatagga gaagagtact gcgcagaaag gttttcttct acgagtttca ttaaagattt
    56521 aaattgatca taatctaaat atttatcttc ttttggcttc aatgaatctt ttcctttgat
    56581 tactgctttt ctagttggat caaacggaat taatccttct tctacggcat ctttcaacga
    56641 cgctctaata tggttattaa acttaataac ggtagatttt acatggtctt tagcatactt
    56701 atttaaaaag cgttgatatc ccactctatc taaatcagaa atcaataccg ctggcatata
    56761 tttctttatg ttcattaacg tatcttcgta ttttctataa gtgataggag atactgttcc
    56821 ttctttataa agttgcatcc aatcctcaaa gtaatctgat aataatagat ttttgcgatc
    56881 ccctttaagg cctttgttca agttatattc taattcgttg gcggcatctt ttgcttctcc
    56941 ttttgtctta aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt ttttgtatga
    57001 aatttcgtat tgccaactat ttccacgttt gactaatctt gccataattg aatcagctct
    57061 ctttctctga tacaatagga actataaaga agcccattgt ttgggtttta ttattttttg
    57121 cacatccttt ttgcttgccg gcgcaaggat gtgctatttg tcgttttaat ggtataattg
    57181 gatgtgtatt agatcatcca atcagaggtg tttattatga ttaaagtgat ttttaaaaga
    57241 ataactgaac ttccagagtt tagattattt gttgattatt ggtatgtctg gttttttatt
    57301 gttgcattgc tgataggtct agatgtcata ttagaaagaa aacttaagta agagttgagt
    57361 taataaattg aattcctaaa tgagatattt tgtagcccat attttgtttg ccgacatcac
    57421 ctaatactat ggagctaatc gttttttcta acagaccaaa atcagataat ctatttaagc
    57481 taccatctat tagctcttgt gaatagccag acatgctgat atttccagtg aaattttcgt
    57541 ttccacgact cattaagcat gctaacaatt gaatctcaat cgatgttaat tgattcagta
    57601 caagaagata atactcctcg ttatcccatg aagcgttgtt aattcgaagt aaacaattct
    57661 tataaagatt tttatacata tctattttat tttgtgcctt tgatttttct acctcgtcat
    57721 ttatcccttc gaatattcca atgagttggt ccttgttctc taagttgaaa acatcttctg
    57781 gaatttgatc gcgaatgttt tctaatgatt cgtttaaatt tttgtagaat ttctcgatgc
    57841 gtttgaaacg cttctcgttt ttagacccaa aataaagtga ctgcacaacg ccaccaatat
    57901 aaggaattgc tccaataccg ccttcaacta ttaattcaag atattctttt ttggttagtt
    57961 tttcattgct attatccata gttacctcct attttgtcat ttcctttaca agcattgtga
    58021 ctcctgatgg cactcttttg tataggtttt attattgtgc acaatctaac tcttagtggg
    58081 gcgtggttca tttatttttt acttacttta ctattaatca tatcccattg agcataggat
    58141 ttttctatgt tcgatcctct tgaactaatt tctcctgtaa aagttacttt atcgcccttt
    58201 gtaaaggtat cagcattgtt taagtccttc gcaaaaatta catatgaaac tttaggtgtt
    58261 gcagaaatat cattccaact tttatcatcg tactctttgt ctgctataat ggctattcta
    58321 gtagtcattg attcgataac agttccttca aaagtatatg ttttaccact aatatattta
    58381 gtaaaagctg tattttgctc agtagctgga gtaatagaat aataactatc taagaaagca
    58441 ccccagtcat gattatttgt ctctaaaaat tcttcaaatt gagttaggct tttattgctg
    58501 ctgttagatg tagtagtttg agtatcagat gatgagcttt cctgtttgtt attgtcattt
    58561 gcctcgtttg atgaggaaag cgagaaacct acggtagtaa aaataatccc gataataagg
    58621 agaatcatag gtgttttctt atttgttttt ttgataaatc ttacaattaa catgattaaa
    58681 cctataataa ataaaacaat accagtaaaa ccaaatatac cgtccattaa tagatcctct
    58741 ttctcttttg atattgaata ttataaacca agcaattctt ttttcttaaa atcgaattct
    58801 tcttgattaa taatcccttc atcta
//
LOCUS       DAYYBG010000007        54505 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000007 DAYYBG010000000
VERSION     DAYYBG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.007"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..231)
                     /locus_tag="ACHAUN_000428"
     CDS             complement(82..231)
                     /locus_tag="ACHAUN_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459160.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1199..1525
                     /locus_tag="ACHAUN_000429"
     CDS             1199..1525
                     /locus_tag="ACHAUN_000429"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8459161.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1533..2441
                     /locus_tag="ACHAUN_000430"
     CDS             1533..2441
                     /locus_tag="ACHAUN_000430"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8459162.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3067..3321
                     /locus_tag="ACHAUN_000431"
     CDS             3067..3321
                     /locus_tag="ACHAUN_000431"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFM8459163.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3863..4021
                     /locus_tag="ACHAUN_000432"
     CDS             3863..4021
                     /locus_tag="ACHAUN_000432"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8459164.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4093..5318
                     /locus_tag="ACHAUN_000433"
                     /pseudo
     CDS             4093..5318
                     /locus_tag="ACHAUN_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5407..5799)
                     /gene="rpsI"
                     /locus_tag="ACHAUN_000434"
     CDS             complement(5407..5799)
                     /gene="rpsI"
                     /locus_tag="ACHAUN_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8459165.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5815..6243)
                     /gene="rplM"
                     /locus_tag="ACHAUN_000435"
     CDS             complement(5815..6243)
                     /gene="rplM"
                     /locus_tag="ACHAUN_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8459166.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6777..7244)
                     /locus_tag="ACHAUN_000436"
     CDS             complement(6777..7244)
                     /locus_tag="ACHAUN_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8459167.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7364..8902)
                     /locus_tag="ACHAUN_000437"
     CDS             complement(7364..8902)
                     /locus_tag="ACHAUN_000437"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8459168.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            8982..9668
                     /locus_tag="ACHAUN_000438"
     CDS             8982..9668
                     /locus_tag="ACHAUN_000438"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8459169.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9869..10396)
                     /gene="msrA"
                     /locus_tag="ACHAUN_000439"
     CDS             complement(9869..10396)
                     /gene="msrA"
                     /locus_tag="ACHAUN_000439"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8459170.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10480..11157)
                     /gene="pgmB"
                     /locus_tag="ACHAUN_000440"
     CDS             complement(10480..11157)
                     /gene="pgmB"
                     /locus_tag="ACHAUN_000440"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8459171.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11154..12282)
                     /locus_tag="ACHAUN_000441"
                     /pseudo
     CDS             complement(11154..12282)
                     /locus_tag="ACHAUN_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(12282..14465)
                     /locus_tag="ACHAUN_000442"
     CDS             complement(12282..14465)
                     /locus_tag="ACHAUN_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8459172.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14462..15487)
                     /locus_tag="ACHAUN_000443"
     CDS             complement(14462..15487)
                     /locus_tag="ACHAUN_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8459173.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15532..16359)
                     /locus_tag="ACHAUN_000444"
     CDS             complement(15532..16359)
                     /locus_tag="ACHAUN_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8459174.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16356..17423)
                     /locus_tag="ACHAUN_000445"
     CDS             complement(16356..17423)
                     /locus_tag="ACHAUN_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8459175.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17449..18285)
                     /locus_tag="ACHAUN_000446"
     CDS             complement(17449..18285)
                     /locus_tag="ACHAUN_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459176.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18282..19151)
                     /locus_tag="ACHAUN_000447"
     CDS             complement(18282..19151)
                     /locus_tag="ACHAUN_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459177.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19221..20504)
                     /locus_tag="ACHAUN_000448"
     CDS             complement(19221..20504)
                     /locus_tag="ACHAUN_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8459178.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20518..22203)
                     /locus_tag="ACHAUN_000449"
     CDS             complement(20518..22203)
                     /locus_tag="ACHAUN_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8459179.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22478..23503
                     /locus_tag="ACHAUN_000450"
     CDS             22478..23503
                     /locus_tag="ACHAUN_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8459180.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24098..27751)
                     /gene="rpoC"
                     /locus_tag="ACHAUN_000451"
     CDS             complement(24098..27751)
                     /gene="rpoC"
                     /locus_tag="ACHAUN_000451"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8459181.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(27830..31447)
                     /gene="rpoB"
                     /locus_tag="ACHAUN_000452"
     CDS             complement(27830..31447)
                     /gene="rpoB"
                     /locus_tag="ACHAUN_000452"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8459182.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32175..33143
                     /locus_tag="ACHAUN_000453"
     CDS             32175..33143
                     /locus_tag="ACHAUN_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8459183.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33161..34066
                     /locus_tag="ACHAUN_000454"
     CDS             33161..34066
                     /locus_tag="ACHAUN_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459184.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34063..34869
                     /locus_tag="ACHAUN_000455"
     CDS             34063..34869
                     /locus_tag="ACHAUN_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459185.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            35026..35994
                     /locus_tag="ACHAUN_000456"
     CDS             35026..35994
                     /locus_tag="ACHAUN_000456"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8459186.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(36311..36835)
                     /locus_tag="ACHAUN_000457"
     CDS             complement(36311..36835)
                     /locus_tag="ACHAUN_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8459187.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            37122..38762
                     /gene="scm"
                     /locus_tag="ACHAUN_000458"
     CDS             37122..38762
                     /gene="scm"
                     /locus_tag="ACHAUN_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8459188.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTS
                     STTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSK
                     RALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(38917..39903)
                     /locus_tag="ACHAUN_000459"
     CDS             complement(38917..39903)
                     /locus_tag="ACHAUN_000459"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8459189.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(39926..40492)
                     /locus_tag="ACHAUN_000460"
     CDS             complement(39926..40492)
                     /locus_tag="ACHAUN_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8459190.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(40506..40922)
                     /locus_tag="ACHAUN_000461"
     CDS             complement(40506..40922)
                     /locus_tag="ACHAUN_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8459191.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(41379..43313)
                     /locus_tag="ACHAUN_000462"
     CDS             complement(41379..43313)
                     /locus_tag="ACHAUN_000462"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8459192.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            43615..44550
                     /gene="pplA"
                     /locus_tag="ACHAUN_000463"
     CDS             43615..44550
                     /gene="pplA"
                     /locus_tag="ACHAUN_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8459193.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            44640..45743
                     /locus_tag="ACHAUN_000464"
     CDS             44640..45743
                     /locus_tag="ACHAUN_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8459194.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            45756..46709
                     /gene="menA"
                     /locus_tag="ACHAUN_000465"
     CDS             45756..46709
                     /gene="menA"
                     /locus_tag="ACHAUN_000465"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8459195.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFTMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(47523..48869)
                     /gene="gor"
                     /locus_tag="ACHAUN_000466"
     CDS             complement(47523..48869)
                     /gene="gor"
                     /locus_tag="ACHAUN_000466"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8459196.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(49003..50256)
                     /locus_tag="ACHAUN_000467"
     CDS             complement(49003..50256)
                     /locus_tag="ACHAUN_000467"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8459197.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(50262..51185)
                     /locus_tag="ACHAUN_000468"
     CDS             complement(50262..51185)
                     /locus_tag="ACHAUN_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8459198.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(51406..53898)
                     /locus_tag="ACHAUN_000469"
     CDS             complement(51406..53898)
                     /locus_tag="ACHAUN_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8459199.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(53905..54372)
                     /locus_tag="ACHAUN_000470"
     CDS             complement(53905..54372)
                     /locus_tag="ACHAUN_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8459200.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        15809 a        11470 c         9997 g        17229 t
ORIGIN      
        1 tatagaaaaa caacctcttt aaatataaaa ggtacgggtt actgattact tgtaaatctt
       61 tacgttccag ttttattctt tctaaaaaat atactcactt cttttcttag acttcttttt
      121 ttgattctcc tctttatggt cttgtccttt tttgccttca aaatatttaa ataaacccct
      181 ttcaaaagca tttctaggtt ttggacaata aggaatgact tttttcttca tataaatacc
      241 tcatttaact atttgatatc atcacattat caaaaaaaat aggtttttta ttaaaactat
      301 cttgaccttt cgggagattt cactgtgatt tatttttatt cccatacttt tttcgttggt
      361 cactttcgta tacctaatct aagcgatcat cggcgacggt gcttttacaa cgaatggcag
      421 gacaaaacaa ggggcggaaa aacttttttg cctatttcat aaaaaacttt ttcctacagc
      481 aatcccttgt tttgtcctac cacacgttgt gaacccaaag cacgccgacg aacgcttagc
      541 tatacgaaaa tgacctccaa aaacaaaaaa gtgaggtgaa tagaaacaaa aagaaattga
      601 aatctcccaa aatgtcgtcc gcctcggaga caacttgctg ctgaaaatgg ttggttaatt
      661 catttaagta ttaaataaga taaatcatca aattaatcgg aaaaatagca tgattggcct
      721 tatcaaaaat aaactgtaaa aacttatatt tctatgagtg gtggaacaat gctaatacca
      781 aattaattta tcgattaccc acctgcacaa accaaagata gcattattag gaaacgaatc
      841 aatcagcgaa tccagcatta taatgaggat actaaaaata aagttttcat aattataaat
      901 ccgccttcct acgaaaggca ccaatgaggt gatgaaaacc ttagagcgct ttctttcttt
      961 ttttattccg tgttaccctt tccttgcagg tccctggtac actacagaac ccgaggaggt
     1021 gagtggcggg gctgtatagc ggcaaccccg cataattata tggtgtcggt catccgttaa
     1081 ggcatcaatg aatggcgcaa acccgtagcc cgtaaactta tctcttccga agttgactcg
     1141 atttcgccgg atgaccagtc cctaccaaac ctgtactatt tttttacaga aggttgctat
     1201 gttcaaaatc tttcgtaaaa attgttgtgg cttggatgtc cacaaaacgt ggatctatgc
     1261 gtgtatcgga atcacagatg cgaatggtcg tacagtgtat aagcaagctc gcttctcttc
     1321 ctttacaaaa ggtctcaatg aattagctga atggcttgct aaatacaact gtaaggatgt
     1381 gtgtttggaa tcttccggta aattttggat tcctgttttt aatattcttg aaaaaacttg
     1441 ttttgtcaca ctggctcatc cgaagtacac caaaccccag aaaggaaata aaacagatcg
     1501 caaagatgca aaaatggatt tgtgatctct ttatgtgcga catgatcaaa cccagtttta
     1561 ttcctggtct tgaaatccgc cagcttcgtg atctaatgcg ttatcggacg aaactgacca
     1621 acatgaaaac aagtgaaaag aatcgtgctt taaactgctt aaccgtttct aatctgaaac
     1681 tagatgatgt ttttagtgat gtgttcggaa aatcatccac ttctatcatc caatacttgt
     1741 tagaacatcc cggtgaatca tttgatgttc gaccatttat ccatgcccgt tgtaaaactc
     1801 ccgttgaaga aatccaagcg gcagttgagg gggcgatttc tgtacctcaa gcgattaagc
     1861 ttcgccaatg cctcaaccat attaacgaga ttgagaagca tcgagaagag atcaatctag
     1921 agattcaagc tcttgcagaa cccttctccc ctgttttgga actacttcgt accataccgg
     1981 gcttagacac tcagccaata acagcgattg cgattctttc agaaatagga ccggatatgt
     2041 ccgtgtttcc aacttcgaaa cactttattt cttgggctgg atgctgtcca agaaacgatc
     2101 gcagtgcccg acgaacaaaa tcaacacgta tctctcgtgc agggcagtcg ttgaaaccgc
     2161 tacttgtaca gattgccaat gctcttctca gatccaaaga acatccagaa tttaaaaatc
     2221 gctatcgtag aatcaaagcg aatcgtggac acaagaaagc tatcattgct atttgtaaga
     2281 tgctcctgac cgctatctgg aatgtcttat caaaggtgga accctattct gctaaaggat
     2341 atctggagcc aaaacctgcg aaagaagaga aagttctgac gcaatcccaa gctctggacc
     2401 ttttaagaaa acgcggatat accattatag atagtgctta aaccaaagtt acgttgattc
     2461 ttcctaaata gactcgttgt cttccatttc aaaaattttg aaaacggtag gcttatttag
     2521 tgtaccgttc ttcaggtatc ttcaaataga tttattttca aacttatttc ctttcttctt
     2581 ttactctcct atagcttagc atttaagtgg gcactcgtgt caccttgcta gatagctatg
     2641 aatgccgtaa taagcctcct agtatatagg cagactgcca attataactg attcaatcca
     2701 cattatgata aaatccacct tttcgtttca tatgtgttga aatatcacat atgtcaaaag
     2761 tgcacgagtc ttttcattca gtgctttgta aatactcaaa attattcctt ttcactttaa
     2821 aaaaaagata attagtacaa aatcagaaat gaggaaagta ttaggatgga ctagaaagga
     2881 gttgttgaat ttgttgcaac agattggtca tttatcaatt aaaaattagg tgaagtttta
     2941 ttcatcactg cagctgctgt ttcccagaga ataaaacgtc tagaagaaac tggtcagtta
     3001 ctgtattatc aagcagcaat tgactacgaa caagcttgat tactaaccaa agcgtttatc
     3061 cacctgttga ttaaatcaaa tcaaaaatca ggtttttata cgttcataaa agcaatctcc
     3121 agtgtactcg aatgtgactg tatcataggt ccttattcga tgttaatcaa aatggggttt
     3181 ccttctaccg aatcattgga cagttttatc aatgatttat caaagtttgg acgaaccaac
     3241 acccaaatcg ttttctcgac tcttgtttcc ttaatggttt atcagttctg gaatgtttat
     3301 catgcgcttt tattaaaata gaaaagagct tttttcatgc gacaaagcag tgatcattta
     3361 ttggcacatg caatctttgt ggatccattt tttgaaaata cgatatttag ttttgaccca
     3421 aaaatcttta aatacgtatt tgataattta tcttaacaac cagataattt tattcagcac
     3481 cgtattatct atattgaatt ctcggaatca agataaattt ttttccgttt ttgtttcttt
     3541 tttaatcttc ttcttgttat taatagtgat taggatgata ttcctatatt gatctttgat
     3601 aattagatat aatttttgaa taacgttccc tgctattcga gctagaaata caagtacgcc
     3661 atgacctagt tagcgacaac tacttagaga tttcttttgt tctgggacaa caaaatgcca
     3721 tgacaatagc agtaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga
     3781 aattcctatg tgcttaccca aagcggttca aaaattttaa actattattt taggagatga
     3841 ttttataaaa tcgaaagcct acttgaataa aaaacaagcc tgtgaatttc taggaattga
     3901 taataatact ttagataagt ggattcgtgt atataatttt ccagtaataa aaattgatgg
     3961 cgtagttcgc tttgacgtag aggatatcaa agatttcatg aatacattca aaaaaaattg
     4021 aaattttctt ctcgtcacat atactataaa tagcgaaatg tactgcctta ctttttaaag
     4081 gagtgaggaa gtttgcctac aaagctttca aatgggaaat ataaaactaa tctgaggtat
     4141 ccaaaaaaat tcagagaaat cactggaatt gcatcagaga agtatcaaaa agtatttcca
     4201 actaggcaat tagctattaa ggccgaaagg gaaatgaaaa agaagattga aacagtttta
     4261 agagaggaga atgcaaactc actagaattg aaaggaaaaa taaaatttaa agatttttat
     4321 gaatcaaaat ggcttcctag atatgaacta ggtcaaacta ctaggtcaaa cagggctcca
     4381 agtaatgtaa caattagtaa tactaaggat atattccgat tgcacatcct tccaatgttt
     4441 ggtgaatatg caatgaatta tctgaattca aatactgaga taatatcaga tgaattaaca
     4501 aagaaatcga aagaatacgc aaatataaaa attatcaaag gctatgttcg ctctatgttt
     4561 gatattgccg aaatactatg ttatatcgaa ttcaaccgaa ctaccaaaat tattcaatgt
     4621 attaccgcgc ctaagaaaat ggctttagaa gaaaaaagga taagggaagg gaatcaagca
     4681 ttatcatcag aagagctgac tgattggata gaagcagtaa acaatgactt aaataatcac
     4741 ttgcttacac ttcacgatta cacacttttc atgcttacct tatatctagg tgatagaaaa
     4801 agtgagactt atgctctgca atggaaatat attgatttta ataaaaatac ggttcgtctc
     4861 aaatatgctt tagataaata tcaaagaaag acacatacaa aaggttggaa agacaccgtt
     4921 attcaggcac cagaagttgt gatgacatta ttaagagaat ggaaatcagc tcaggctgaa
     4981 caattgttga aactaaaaat tatacaaaca cctgaccagt acctctttac ctattccaaa
     5041 ccttctggag aagtaaattg tcctgttcac gctgactact taaactatcg aatcaatgca
     5101 ataaaaaaag gcatcctgaa ttggctcaat tatctccaca caaacttcgt catacctatg
     5161 caactatagc tagggaaggt ggagcaaata tgaatcaaat ctctaatgct ttaacgcact
     5221 cggatatttc tactacaaaa atatacgtga acacacctga cgtagttgat aaatctgttt
     5281 ttgaagcatt tcaaaaagga ttgaataagt gtgattaaca tgtgttctca aatgtgttct
     5341 tattataaaa aaagacactt tccaaataat tgaaaagtgt cctgaaatat tgatataaaa
     5401 agaatattaa cgttttgaga attgtgaagc tttacgagct tttttaagac ctggtttttt
     5461 acgttcgacc atacgtgcgt cacgagtaag taagccagca cgtttaagag ctaaacggaa
     5521 gtcaggatca acttctagca atgcacgagc gataccatga cggatagctc ctgattgtcc
     5581 agcgtatcct ccgccgttta cgttaacaaa tacgtcatat gcgccttttg tttcagtaac
     5641 agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt cttcaacatc
     5701 ttttttattt acagtaattt tgccagttcc tggtactaga cgtacgcgag caactgcgtt
     5761 tttacgacgg cctgtgccga tatattgaac ttgtgccaat gaaattccct cctattagat
     5821 taagtttgtg atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt ctgctccacc
     5881 gtatacgttc aattttgtaa attgtttgcg gcctaaagtg tttttaggaa gcataccttt
     5941 tacagaagtt tcgatcaaac gacgagaatt tttagcacgc aattcgccag cagtgattga
     6001 tttcaatcct cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg cttttttacc
     6061 tgttaatttc actttgtctg cattgatgac gatgacgaaa tcgccagtat ccaaatgagg
     6121 tgtgaaagtt ggtttatttt taccacgtag tacagatgca acaactgttg aaagacgtcc
     6181 caatggaaca tcagttgcgt ctactacata ccatttacgt tctacttcgc ctggtttggc
     6241 catatatgtt gtacgcacgt tattttcctc caatttaagt tctgatgttt gacatacact
     6301 tcgagtttcc ggggctcttt gtggggcaaa caataccatt taacatatta cccgctaatc
     6361 tagtcaatgt caacgttttt ctgcttgatt gaaaaattta tatgaaactt tacttctttt
     6421 ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag aggctgtgac
     6481 aaaagtcttg tcacaacccc ttattttgaa taaacggtgt tctcctgcca actcctcggt
     6541 cacaagtcac aatatccata aaacataaga agctgttttt taaaattgtt tcttgtgact
     6601 cggagctgaa cggcaggctt acaacctctt ggtaaacggt gttcaatcag ctgaccctcg
     6661 gtattagctc aaaaaacggc aaaatatgag gaactatttt gacgtttgtt tctctaatac
     6721 tcaggtcaaa acgctgattt cacaacctct ttttcggtca agttcaacaa cagtttttac
     6781 gcgtcgatct ctggcgcttt gcccaaatga gcttggatca tccagatacg tttttctgtg
     6841 gctgttttga attcgataaa gatatcttgt gttggatcgt cgccttcttc tccagaaact
     6901 tcgattcctt tttggtaaag atctgctaga taacgatagc ctgcaacaag tgtttccaaa
     6961 cgttctggaa tagtacgatc ccagcggcca atttcatcag ggatttttgt gttatctgca
     7021 aattcacgca atgtagaata aggtgaaccg tctaatgtga tcaagcgttc ggatacttca
     7081 tctaactgag cgttcaaatc atccatgaac tcatccatca ttgggtgcaa tgtaaggaaa
     7141 cctggtcctc tcatgtacca atgtgtttgg tgaacaacaa cagacatttg gcttagatct
     7201 gcaactaatt gatttaatac ttctttggtt tgttggaatt tcatctttct tgcctcctaa
     7261 cattttctta cacttaaaat aatagtagac tttcaatgaa aagtacaaca aaatcattta
     7321 cctgagaacg attttcagtt cgctttttta tccgtaaagc caatcatttt tcagaatcag
     7381 ctaattcttc tcgcagttga gcaaattgct gataagtctg tgctaaatgc ttttgtgtcg
     7441 aaacaaataa tggatagatt ttctgatatt ttttatgatc agtttcttgt actccaactt
     7501 gctctttcaa ttctatcaat tcctgtgctt cactgatact ttggatcttt ccgatgcttc
     7561 tccagcctaa aatcacagct cctaatgcgg atgcttctgt gacagtaggg aaaacaagcg
     7621 ggtaacccag tacatcagcc atcatctgac gaaaaatcgg agattgagca aacccgcctg
     7681 tagcacggat ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc cgataaaggt
     7741 tgagacagat cccttctaga atcgccttca tcatttcttt ttctgtatgt tgccgctgta
     7801 aaccgataaa actgccaaac gcatctgcgt cccaaagcgg tgctcgctca ccaagtaaat
     7861 agggatgaaa aatcagacca ttggcacccg ctggcacaaa agcgatagcc gaaagcagtg
     7921 catcatatgg attttctcct ttttctttag cagattgaac gacttcctgt aaaaatgtat
     7981 gccctgccca atcaaagatg ccagcaccat tgctagtcgc tcctcccact acccaatgtt
     8041 tctgatccag aacataacaa aacgtctcca tttctggatg gaactgaggc tgagtagtaa
     8101 taaaacgaag tgctccgctc gttcctacgg tcaaagcggc gatcgtatca ccaaaagcac
     8161 ctaaccctag attagaaagc ggcccgtctg cccctcccaa aacaaatgat atatcatttg
     8221 gcaaacctag ttctgctgct gtttttgatg gtagacctgt tacttgatag gtggaaggaa
     8281 agacttgcgg caattgctct attcgaatgt tcaagtagtc aagcacttct tctgcccatt
     8341 ggaattcatg gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg ctgatcagtt
     8401 cgccaaacaa tctataaaag atatagtctt tgataccgac aaaataagca gtttttttga
     8461 ataattcagg ttggctgttt tgcagccact tgattttgtt caatggtgtc attggatgaa
     8521 tcggcgtccc agtctgccga taaaattctt tgccaagcac ggtatatttc agtttttctg
     8581 cttcgtccgc tgctcggtta tctgcccaag tgatcattcg agataacggt gttttctctt
     8641 catccaacaa tatcaggcca tgcatcgcag aggagaagga gacaaattga atgattgtgt
     8701 ctgaaggcaa ctgtttaatt acttctttta taacggtgca gacaccttga aagatcaatt
     8761 cctgatcttg ttctgccatc ccttgtgttt cttggatcaa agggtagcga aaataagcgg
     8821 atgctgcttc ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg gttccgatat
     8881 ctaatccgat tgaaacgttc attttttcac ctcaaatttt atgtatgtac aaaaatttag
     8941 tttaagcttg cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt tattttatca
     9001 ttggttgctg tatcggatca tttttatgtt taaccgccca gcggattccg ttaggtcact
     9061 ctattattta tcctagatct cattgtgtca aatgctgtcg ttcgctttct tggtatgaat
     9121 tgattccgat tctctcgatc atcgtccaac gatttcgctg ccgctattgc aggtgtcggt
     9181 tgcccttcta ctatttgttg gcagaagcct tatgtggtgg tctttttgcc tggttcttta
     9241 ctttttcttc cgcaagaaat ttttcgacgt tcatatggat gctttcggct ttgctatttt
     9301 cgttgatgga tttattttac ttcatggtcc attctcacac gctattctgt tcttgggcaa
     9361 ttttatggat tttttggttg caggccggcg tcttccaatg gcaaagtgtg ctgttaacgt
     9421 ttatcgtagg cggtgtgtgc cttcgctatg gtcaatccta tctcggagct ggcgatactc
     9481 tgttgatgct ttcttggagt ggtggcttgt ctctagaaga gttgcttcag atcttgtttc
     9541 ttgctagtct gtttggaata tccgtttttt ctgtttactt tatctgtcat aaaaaaaagt
     9601 tggaaaagct gcctttcatt ccttttttga gcagtgcgtt gctgattgtc cttcatctta
     9661 ggtattgaaa aaaggttgtg acaaacgact tgtcacaacc tctgatatat agtgttctct
     9721 taccaaatcc ttgtgacttg gagctaggcg acaggttcac aacctctcca ctaaatggtg
     9781 ttcaatcagc tgaccctcag tattagctca aaatgctggt tttacaacct ctgatatacg
     9841 gtgttccata agtagctttt tcggcttctt atttcttgac gccccaatgt cgacgaagga
     9901 aacctgcacg tccagaacct tctcgataaa gattgtaatg gagcttgttt ttcttgtaat
     9961 aatcctgatg gtaatcttct gcaggataaa attcgcttct tggttcgatt tttgtcacga
    10021 ttggcttatc gaatcgcccg ctgtcttgca atgcttgttt tgaacgctct gcaatatttc
    10081 gttcttcttc atcattatag tagatcactg gacgataaga atcaccgcga tcagcgaact
    10141 gtcctaatgc gtcagtcgga tcggtttgct gccaatagat ttccaccaac tgatcataag
    10201 aaataacttc tggatcatat tcgatctcta ctgcttctgt atgccctgtt gtgcctgttg
    10261 ttacttgttc ataagttgga tttggtacat gcccgccagt ataaccggat gtcacagaaa
    10321 tgatccctgg ctgctcttca aatggtttga ccatacacca aaagcatccg cctgcgaaaa
    10381 ctgctttttt tgtcatataa aatccccctt ttttctcatc ataccttgat ttccaaaagg
    10441 aggataacgt tttgcttgtg acagaaaaaa cggaaactat tatatacgtg atacggcttc
    10501 tgataaatgc caaaacgaac gtacttgatc aaaaacggtt aaatcaggaa tcacatattc
    10561 agcagaagga agatttatgt tttcacctat tcccaataca tgcatgttac cagccaaagc
    10621 tgcttgacaa ccagcttctg aatcttcaat gaccaagcat gcatttggcg gtacattcaa
    10681 ttgctgtgct cctttgagaa aaacttctgg atcaggtttt gcttttgata cttgcgtgcc
    10741 gtcaataagg acatcaaaat aacttgtcaa attcgttttt tctaaaatga ggcgtgcatt
    10801 tttgcttgct gatcccaatc cgatcttgat gtttgttttt ttcaagtact caagaacagc
    10861 tattgcacca ggcaaaacgg atgtttcatc cattttttga atcgcttgaa gatatagatt
    10921 gtttttttgc tcagcaaagg ctgatttctc tttttctgag aatttctgtt ccatttttcc
    10981 aaatgacagc aatcgctcta gtgactcggt tctggatatt cctttcagtt gctcgttttc
    11041 tttctcagtg aatggaatgg acagctggtt agctaaaacc agccacgctt cgtaatgata
    11101 acgagctgtg tctacaagaa cgccatctaa atcgaatagt gctgctttga ttttcatcga
    11161 ttacctcctt gaaaatgaac gacttgaagt aaagatgcga tgtttttagc tgtgcgatga
    11221 agattatcag tggcttgctg taaagcttct tgtaaaggca cagctgcagg aaggatcgga
    11281 aaaacggccg aaatgccaac atctgctgct ttttcgcttc ctttaccaac acttccgcaa
    11341 atagctatca caggaacgcc ctctggtact cttctagcaa tcccaactgg cgcttttcct
    11401 tttagacttt gtccatctag acgtccttcg cctacgatta cgaggtctgc ttgctgtacc
    11461 cgtatcgaac tccagtaaat ccaatacgta gtcgatgcca gagactattt tccctgaagc
    11521 aaatgctaac agtcctgcac ccattccacc accagcacta ctgcccgatg cctcgattac
    11581 ttgaggggca aatctcatat aaaaatcatg catggcttgg tcagccactg ctaattcatt
    11641 ttctttcaag cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc cgcataaaag
    11701 attagtaaca tcagtaatga cagtgatttt ggctttaatc agctcaatag gcacgttcgt
    11761 tttatcaact gtagcaattt tacctagatt ttctcccagt gcttctactt tttgattatc
    11821 ttgatcgtaa aaagcataac ctagaccttc tgccatgccg atgccgccat cgttggtact
    11881 acttccgcca acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt gtcgtatcag
    11941 ttctcctact cctcgcgtgg ataacctaag tggctgttgt tggtttagtg ggacatcttt
    12001 caatccgacg atttctgcca tttcaaaaat cgcagtttga ccgtctgaag cgtactggat
    12061 ttctttgttt ttgccaaatg cattagtaac gactgctgtt tgccgcttca accttaaact
    12121 gtctgctaat gatgaaaccg ttccttctcc tccatcgcct ataggtagca gatcatattc
    12181 agcctgaaca aaaacttctt gaaaacctga ttgaatggct tgtgctgcct ctaaggctgt
    12241 cagactttct ttgaaagaat ctggcgcaat cagaatacgc attattttcc cctccgttcc
    12301 aagatcagtt ctttttccag ctggtattgg tctccattga caaaaatagg taatgtttct
    12361 ttcgtctctt tttttattac tatttgtttt tttgtcaaaa caaatagcaa cctgtgattt
    12421 ttccaaataa aaggaaaaga aattttttcc catgctgaag gaagtcttgg agaaatagaa
    12481 agagttccat tcgttactgt aatacctgaa aaaccaaata taactgctag ccatagtgct
    12541 cctaatgaag cagcatgaat tccgtcatct gatgaatgcg gatctggtcc taaatcaatc
    12601 aggcacgctt cttggaataa atgatacgcc cattccattt cttggatacg gcaagcctct
    12661 atggcgtgaa tcgctttact caacgatgag tcgtggattg ttttgttttc atagtaatgc
    12721 aaattcttag aaacgattgg ttgagaaaaa tactcaggta ataagtataa cagcatgaca
    12781 agatctgctt gcttgctgac ttggagttca tttacttcgc tacgggcata atcaagtaaa
    12841 atagactggc tgccaggacg ttctttgtaa gtggtcaaat caataactgg cttactgaga
    12901 tacgtgtcat cttgcggaat cagctgttct ttgttaggca ctggcaaata taacttatct
    12961 aaaaaatttt gacattcttt caaaaaaagc ggatcgccag cttgatattt aacagctaag
    13021 gccacattat aatgcgccag atagttcgta tatgcattat tgtcgatata ttctgtgtat
    13081 tcatctggac caatgactct gtgtatctct aaatgatcat cgacttttac agctctgctc
    13141 agccaaaaag ctgctgtttc atgaatgagc gtttttccct ttgtttctaa aaacgattga
    13201 tcctctgttg cttggacata ctcttgaacg gcataagcaa ggtctgcact gatatgatgt
    13261 tctgcggcag ctgaagcaac ttcttgtctt gttccggttt tgatattcaa tgcagcaaat
    13321 ttgggtgtct cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc accttgatag
    13381 tgataatctc ttgctttttg ccgtgcgcct tctaatcgat cataacggta cagcagcaaa
    13441 ttctttgcta cttcaggaaa ctgatataaa aagaaaggca ggataaaaat ttccgtatcc
    13501 cagaaaacat gccctttata accttcgcca gttagtccct tcgcccctac actaacactt
    13561 ggatcattgc ggggagtcat aatctgtaaa tggtaagcag cgaagtccat cgctaactga
    13621 tcgaaagaag aatcgccttg gatcgttatc cgatgctgat gccaaaaatc tgtccaataa
    13681 gacatcgact gctctaaaat ttctgtataa ctaacgcttt tctgggcttg ggcaatactt
    13741 ttcttaaacg ggtctgtttg ctgaagagag gtagagagca cgatgatctt ctccagcgta
    13801 aaggacgtat tttttctgat cttttctgaa aaggaagcgg tgatccggcg attttttgct
    13861 tggaatacac ctgctttatt gaatttagct gcaatcccaa tatcaatttt actttctgtt
    13921 gtctggtagg atgcactgat caaatcgtca ccaaatactc gcagttcttt ttcaaccaac
    13981 tgctgtgtac caaaattggt ttgctgtcca tcgatacccg ttttcacatc tacttggcag
    14041 tcttcggtca acggcttgat gaccactttc agggctgcct gatggagatt attttgatca
    14101 gcaacacgac aagtaatgac ttccactttt tttccttgag cagtttcaat gagcgcatag
    14161 cgtttaagct ctcccatttc taaatctagt gcccgctcgt atgcaagaac tttcatcgtc
    14221 tcaagagaaa aatgttcacc gtcgattttt atttcccatc tgatgacatc tgggagattc
    14281 actaactcgg ctgattctgc tcctaaaggt cggttatata caccagctgc aaacattccc
    14341 cttacttgat tccgatagtc ttcctcctgg cttcctctga taccaaaaaa tccatttcca
    14401 atggtcatct gactcgcata cttattgata tactctaaat caaactgttt ttcttctact
    14461 ttcataaggt aataacttct cctgtttctg ccgaacgata taaattttcc accagattct
    14521 ggatgacata tccttgccag ccatctgcaa tcatctgagt ctggtcaccg agcgcccgat
    14581 tcacaaaatg agccatactt agttcatgtg ggtcattaac tggcgtggtc ttttgtaagg
    14641 aaaccagctt tccatctaca tcttcgaata gttcgcctgg atacagatta gcacctgcct
    14701 gatcgccaca aaaagccaca ttcagtttgt cttctctgat attcaaagca aacgaagtat
    14761 tcagttggat gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca tcttctactg
    14821 tgaatttgct tggatcccat ttcccgaatt tccctgaatt ttttttcgta ccaatttttt
    14881 ggaagctgta cgcccgaacg ctttttactt taggaaatcc agtaatatac atcgctgtat
    14941 caagcatgtg aatgccataa tcgatcaaag gcccgcctcc ttgaagttct ttgtcgataa
    15001 acgttcccca gcctggaaca ccagaccgac gaagcgcatt cgcttctacg taataaatat
    15061 ccccgagaac atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa cgatgctgga
    15121 atccataagc caaaatctgc ccgtttttat cggctgtctc tgccatcttt cttgcttgat
    15181 cagctgtcat agctggtggt ttctcgcaca tcactgaagc attagcttcg agggcagcca
    15241 atacatgttc gaagtgaaag cgattggctg tacagacact cacgatatcg ggagaaatct
    15301 cattcagcat ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt cggcaaaagt
    15361 cttctgctcg ctggctgact ggatcaacca cagcggccaa ttctaagaca tctgatcttt
    15421 tttgataata agcagcatgt actttttctg ctacttgtcc agcgcctatg attgctactt
    15481 tcttcattta tttctctctc ctttgcttat ttacttattg ttattctttc tctattcttt
    15541 ctctaaatca tttaaatgac gcgctgtttc cagacagttt ttcaagtatt gcaatgattc
    15601 ctgataaatt ttcacttcat tttctcctcg caaacgacat tcatatacca tataaccttc
    15661 atactcattt tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc cagatcctgg
    15721 ttggtagcga tgattatctg ctaaatggat atgacccaca tactcttttt gcgtagtcaa
    15781 tgcttttgaa atatcatctt cttcaatatt catatgataa aaatcagcga tgatctgcac
    15841 atgtttcaat tgattagagt caatgtaatg acgcgcatcc gctaacaaat tgatcatatg
    15901 gtcttggtat cggttcaaag gctccaaata aataaccgta cctgtttctt cggctacttt
    15961 atctaaataa cgaagtgatt cgctgactgc tttatagtct ccttctttgc ttcggggtga
    16021 aaccatcggc ggtaatcgat aagtgaacat tccccaagct gctgggataa cgattccttt
    16081 gcctccaacc tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac cattaagacg
    16141 ccgttcttcg ataaagtcac cgatccaccc atcgtagccg ttacaagctg tcactactgg
    16201 gatccctgtt tctttgattg cctgtttgac tgtgtcgata ttttctatta acaacttacc
    16261 gtcgatttcg tattcttcaa atccacagtc tttgataaat ctaaattttt ctaaaatgtc
    16321 tcttgggaaa aatgcttgat cttgcgtacc gaatttcatt gttctcctcc tcggacttga
    16381 acgcccattt tgatactttc ttctgggtgc tggtctacat aacgcatgta agcttctgcg
    16441 ctttctgcaa atggcacaac tggatcaatg atttcttcac aatccaagta accgttcatc
    16501 aataattccc aacatgtgtc ctcgattcgt ttacggttcc atcttgggta atctgggttt
    16561 ggttcactag atgctcgagc aaagacgatt tttgcattat tgaaatgtgc ttcgcgacca
    16621 agattaaacc cagctggaaa aggtttagca aaggcaacat aagaaatgat cccgccatag
    16681 ccgatccctc ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc aataactgaa
    16741 tctactccac gtttatctgt caattccttt agacgaaaac cgacatctgc tttcatagga
    16801 tctagcgtgt aatcggcacc gtatttttct gctaattctc tacgctgcgc tatgggatcg
    16861 atcccaacga tggtagtagc acctgctttt ttagctaatt gaatcgcaat ttgcccgata
    16921 gcgcctaaac cgaagattgc tacagcatca cctgctcgaa catgtgcttc ccggatacca
    16981 cctaaagcga attgagcagg atcgtaacat actgcatttt tccattgatc caaccgatca
    17041 agtttacgta gtttataatt atcaacaccg tttgtaatca gcgtttcacg aatgggtcca
    17101 taagtgcaga ctaactctcc tttttgatac tctgtcacca aactgcccgt ttcttcgatc
    17161 gtaccgacaa tcatatttcc taattgaaat tcgccaaata ccacaccgcg ttggcttcct
    17221 tcttctcttg gcaagaatag gcgccattct tctgaaaatt cttcatcgat aaatggactt
    17281 aatcctctaa agtcgacaat ttctgttcct ggttttggag tagcgaaaat tactcggata
    17341 cgtacttcgt tcttttcgat ctcacggtct tcgtaatctt tgaatccggc tattcttggt
    17401 tctaatgcaa ctaatttttt catcttttct ttcctctttc ttttcttttt atccttttac
    17461 gccgccttcg actaaatcac ctttaataaa tcgttcagat aacgcataca taatgatgac
    17521 tggtaaagcg gtcaccaatg aagcggccat cattcgtccc caaatgtagt ctggtgtact
    17581 gaataatgtg ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca ggaaaatcga
    17641 agcaaataaa taatcattcc acgaaaccat aaagcaataa acgaagacag aaatgattcc
    17701 agaaatagcc aatggtataa tgatccgaat aatgatctgg atccggctaa gaccatcgat
    17761 cattgctgct tcttcaatat cagtcggaat cgtgtcaaaa taacttttta gcatgaatat
    17821 cgcagtagga agtgtttgaa cgaccatcgt gataatcagt gctgtattgg tatcatacaa
    17881 acctagaccg gaaatgattt taaataatgg aacaacgatc aaaataccgg aaaacatata
    17941 aacggtgtaa aagctagcat tgattttcga gcgagctttg aagcgtagtt ttgacaaagc
    18001 gtatgccccg ctgatcccaa tcactactgc agttacggag gctatcaagg agatcactaa
    18061 actatttttg aaatacatga tgaatggaaa aatctcagca ttgaaaatat cctgataatg
    18121 ttcaaaagtc cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt cttgtgaatc
    18181 cttgaatgat gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta atagcaacaa
    18241 aatcgtcgcg taaaataaga ttttcctgct ctttttattt ttcatttcaa gtccaccttc
    18301 tttcgtgaga gcaagacgac cgctgcaata atgatgaaca atagaatcga tatagctgct
    18361 gcttttccaa gatcattgaa cgcaaaggct gtctgataca aataaacgcc taagatattg
    18421 actttattcg tcagtagata gacatctgta aacatataga acatccagat agatcgtaaa
    18481 acaatcacag ttgctagtac tggtttgatt gccggcagtg tcacgatctt gaacttctgc
    18541 caagcatttg caccgtccat ttcagctgct tcataaagcg ttttgtcgac tgtttgcaaa
    18601 atagccaaaa atgaaataaa cgcatacgga tagtatctcc agatagcaaa caaaacgacc
    18661 ataaagaagc tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa aagctgcaac
    18721 ctatctacta acagatcatt gacgattcca taactattat tgaacatata tttccaagca
    18781 aaaatcaaag aaatagatgg cgtaacataa cttaataaaa tcagtgaacg caccgttttc
    18841 ctaaatctga acttgcggtt aagcaaaata gctacggcta atcctaaagc tgtactgcct
    18901 gctacaacta agaatgtgta agcaactgtt aaacctaaag aacgataaaa ttcactatca
    18961 cttaaaattt caagataatt gcttaatccg ataaaaacgg cgtctgagtt aggattcagc
    19021 ggcatatcga aaaaactgat ctgaatattg gaaagcattg gaatcagcac taaaacagct
    19081 aataaaagca tgcttggtgc taaaagtccc atggctaatt ttaagtcaga atgtttcctt
    19141 ggacgattca ttgaaacctc cccctgataa tctgccaata gctatctact tttttcgaac
    19201 accattggca gaacttcttg ttatttcgta tttttttgtg cgtcagctaa agcttgatcg
    19261 acatcttcgt ttccgacagt tacttggttc actgcttttc cgatcacacc tgagcttgtg
    19321 atatctccca ttttcgtgaa attcttttcc ccgactagtc caaagacttg tacttcatca
    19381 aaagcactag caatctcaga tggcagctcg ccaaaagact tgatgacttc attttctttg
    19441 taaccatcca gttcgactac ttgtttattc actggttgag caccaccagg agacatcaag
    19501 acccactttt ccatgttttt cggttgagat aagaaagctg tgaatgcttc agcagcctct
    19561 ttttgtttct cgtctagacc agaagaaatc gtcaatccag atactgtgcc atagacagct
    19621 tgatcttttt gtgttgggat agcaaaaccg atattttctg agttccccgc ttcataaaca
    19681 gaaggcaaaa tgtaagttga atagattgcc attggtgctg ttccattcat gaaagcatct
    19741 ttgatttctg tcacatcatt agagcctggc atcgtgtatt gggataagtt ttgataaaac
    19801 gacaatgctt ctctcatctt ttccgtatcg atcgttacct cgccttcatc atccaaaaca
    19861 tttgctccat ttgacaaagc aaattgagag aacgcttgct cactcattgt tccttcagca
    19921 gtaggcatag caattccata ttgtttattc tctttatctg tgaaatgttg agcaattttt
    19981 aaaatatcat cccaattttc tggttctgaa aaaccagctt ctgataattt ttctttgtca
    20041 taccaaatac cttgaaccca gccgctaatt ggtgcagcaa tatagctttt tccatcttct
    20101 gaacgaacaa ggtttttggc tccgtcataa taattatctt ctccagcttc ctcgataacg
    20161 ttttggactg ccgtctgatc aatcagttca tctttatcca tcactttagc aaaatcctga
    20221 ctgacttcca ttacttctgg taatttaccg gaacgagcta acgtaacgat ttttgtattg
    20281 aacgctgatt cttctaccgg cacttgcttg attttgatgt ctggattttc tttttcaaaa
    20341 tcagcaacca gttcattgat cacacttaat cgttcctgtt cgacagaaga gtgcatgaat
    20401 tgaatcgttg tctgttgtga ttcgttagag ctttgacttt caccacaagc agtaagaaga
    20461 aatccagtag ctagcagaca ggtagtaaca ataatttttt tcattttttt ccacctctta
    20521 ttttattatt ttcagccaca tatattcata cggctggaat atcaactttc gttcttgact
    20581 gcaatggata atttgtccag aaaacaggtc taggtaatct ccttttggta aaagataacc
    20641 tgctgattga ttggaaaaat tatggataac taagactttc tcttccttcg cttcgcgaat
    20701 aaaactgata aaagaatctg ctgagtccag taagtaaatc aaactattcg gatggaaggc
    20761 tttttctttt tttcgaatag atagcatctg ggtcaattca ccatatactg ctgttcttaa
    20821 tggatctgtc tgcaactctt tctcaatatc tgctaacaaa aatttttgcc gattgatttt
    20881 tcgattctct cctgtttttt ctacgccttt gtaatcattt cgacttccca gcacactttg
    20941 aatataaatt gccggcacgc ctggtaacgt taccaaaaat gaatgagcag ctaaaaagcg
    21001 tttgcttctc cattccgtcg tatctgttgg agaacttaac gcatctagat acgtagcgtt
    21061 tatctcatat ggactggtcg ttccatctgg gtttttcttg tagtttactc gggcaccctc
    21121 tttcttcagc tgttccacta attcaaggat cgttttttcc ggcagaatac ctcgtaccgg
    21181 attcaatccg ataccgtcat gggaggctaa aaaattaaaa aaagttgttt ttttcgatgc
    21241 gaaatcgatg gtttttaacc attctttcaa cttacttgta ttacccgaac gaatcgcttg
    21301 aagggtcaaa ggtggtagcg aaaactgata gaccatctgt gcttcattct cgccattccc
    21361 aaaataactg atattatctt tgtgtggcac atttgtctca gtgatcaata ccgttcctgg
    21421 agctgcttca tcagcgattg cacggaataa ttggatgatt ttatgcgttt ctggcaggtg
    21481 tatacaagaa gttcctagtt ctttccataa aaatcccaca gcgtccaagc gaataaatga
    21541 agcgcctttt tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa cttctggatt
    21601 tttaaaattc aagtctactt gatcttcgct aaaagtcgtc catacgtgtt tttctttccc
    21661 gtttttgtca atgaacgttg taagtaacgg gctggtcctt ggtctagtca cgctgcttgt
    21721 atctgtatcc agtggcaaat caataaaaaa atctttgtac gtatcattca agtttaaata
    21781 ttcctgaaac caagtacttt cggcagacat atgattgcag acaaagtcga acattaaccg
    21841 tgtttcgctt ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt tgattgcctg
    21901 ataatttaca acggagaaac catcatcaga tgtatatggg aaaaatggca agaaatgtac
    21961 aactctaaat gtatctgcca aatacttttg ataaaatttc ttaaatgtac gcaaagttgg
    22021 ttgctgctct tcaaaaaatt gatccccata agtaatcaag aaaatatctt cttcgtccca
    22081 atgcagtttg cctgcctggc ctttcttctg ctgataaaca ctgacttttt gttcaatcat
    22141 agaggcggtt ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa gttttgtata
    22201 catcggattc ccctttcttt tttgggagcg ttcccaacga tttgatcata ttatggaaaa
    22261 cgcttactgt caatagcatt tttttaaata attttgatat acacaaaaat acaaataaga
    22321 aagcttatgt atcaataatt aatagcttct taaaaaaata tggtctctca tgatcatgca
    22381 agctattcct aaattttcaa aatcatagac aaaaaagaat gtctattgta ctatggaatt
    22441 attctaaaac gctcccaaaa aggaggtgca tataaaattg aaacctacta tttatgacat
    22501 tgcgaaaaaa gctaatgtct caaaatcaac tgtctccaga gtattaaata accagccaaa
    22561 tatctctgat aaagcaaaag caagtgtact tcaagcaatc aaagaattga attatgaacc
    22621 gagcaaaatt gcaagaggac tgtcaggaaa tggttttgac gcgattctag cattatctca
    22681 tcgtacttct catacgacct atggcaatcc ttttttttca gatgttattc aatccatttc
    22741 tgccgtttct gaacaaaata acttcgactt gattctacaa acggcaaaaa caccagaaga
    22801 agagctagaa aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa ttattatttt
    22861 aagctctacg atggatgaaa cacttctttt tgaattggat caatacaata ttcctattgt
    22921 ggtaatcgga aaagtcggaa acgactacac gaacatctac tcagtggaca cggataattt
    22981 ccaggatagt tacgatttgg tggaaacact gatcaagaat ggacaccgtc atatcggctg
    23041 tctccatgct ccgacagaag tttatgtatc caatgatcgt ctcaatggtt accgtcaatg
    23101 cttatttgat caccacctag atatccgtaa tgaatggatt atagacggcg gctttcgctt
    23161 agaagatagc atggcagcaa tgaatgattt aatgaagcta cgagaaagac caactgcgat
    23221 tttttctacc gatgcattaa aatcattgag tatttacaaa gtggcagaac aatctggaaa
    23281 gcagatacct gatgatcttt cgatcgcagg attcagtgat cccttattgt cctccctatt
    23341 ttcgccacag ttgactcgga tagacattcc gacaaatcag ttaggaatca aagccacaga
    23401 acttttattc caactaatcc accaccaata ccctgaagaa aaaagaatca ttctttcaac
    23461 cgagattact ctcacagatt cgatccaaca acaagaaatg tagaacagaa ttataaaaga
    23521 acagaaacac tgaggataag tcagttactc tgttcttttt tcaatcactc atccctcgtt
    23581 accccctccc ggtattttag cgaatttttc ttattttcca tctgttttag atagctgtgg
    23641 ataactataa tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac tatttttaac
    23701 cacgttgtgt ataagttttt atagttcgtt tttttaaaca catttaccct tatttcaatc
    23761 catgttattt ggggatactt atctctaaaa cataaataga ataacagaca tgtctactgg
    23821 ttttgtttta ttcgaggaat tattcaataa tagctagcaa aatattaaaa ataaccgctc
    23881 tatcttccca tcaatttatt ttgcattcaa aaaatgatct atctttactt ttgttgattg
    23941 acttctcttg gaagatgatc gttccgatac cttaattgga tacgaaccga tgttgttaag
    24001 atcctttccc acttttcttc tagttagaca aattatttta gacaaaccaa aaaagaggtt
    24061 gtgacaataa tttgtcacaa cctctttttt aaaatatcta ttttgtttga ttgttcattg
    24121 cgtcttctgc agccatttgc gcttcaatat ctgaaatact gtagacattt tcgcttgcta
    24181 caccgacttc ttttggttcc atgtcacgat aacgttgcat accagtacca gctgggatga
    24241 tcttacctat aataacattt tctttcaaac caagaagagg atctttctta ccgcggatcg
    24301 cagcatccgt caagacacga gttgtttctt ggaatgatgc agcagataag aaactatttg
    24361 tttctaatga tgcttttgtg atacctagta agacaggtcg agaagttgca ggaacgcctc
    24421 ctgagactag cgtattgtag ttttgatctt taaagtctgc gatgtctaat aatgtacctg
    24481 gtaagatatc tgtatcacct ggatccatta cacgaacttt acgcagcatt tgacgaacca
    24541 ttacttcgat atgcttgtcg ccgatttcta ccccttgcat acggtaaaca cgttgtactt
    24601 cacgaagcaa gtagttttca acagataata catcgcgaac ttgcaacaat tgttttggat
    24661 cgattgatcc ttctgtcaat ggtgcaccac ggtggatcat atcgccttct gctactttca
    24721 tacgtgctgt ataaggaaca gtatacgtac gtgtatctgt ttttcctttg attgttactt
    24781 ctttggtacg tgttgcagga tcttctgaga tatcgatgac atcccctgtt acttctgtga
    24841 tcactgcttg ccctttcgga ttacgtgctt caaagatttc ttggacacga ggaagacctt
    24901 gagtgatatc gtctccggca accccacctg tatggaatgt acgcatcgtc aactgagtac
    24961 caggttcacc gattgattga gcggcgatcg taccaactgc ttctccgact tcaacgtcag
    25021 agccagttgc aaggttgcgt ccgtaacaat gtttgcagac accatgtttt gtattacatg
    25081 tgaagacaga acggattgtc actgtttcga cacctgcatc aacgatttgt tttgcgatgt
    25141 cttcggaaat caattggttt cctgcaatga tcaattcatt tgtttcagga tgacgtactt
    25201 ctttacgggt gtaacgtccc actaaacgat cttctaatga ttcgatgatt tcatttccct
    25261 cgcggatggc ttggatttca agacctcggt cagtaccgca atcttcttca cggatgatta
    25321 catcttgggc cacgtcaacc agacgacgag tcaagtaacc tgaatcggca gtcttcaagg
    25381 ctgtatcggt catcccttta cgagccccgt gagttgagat aaacatttcc aagacggata
    25441 gtccttcacg gaagttcgaa atgattggca attccatgat tcgaccatta ggagcggcca
    25501 tcaaaccacg cattccggca agctgtgtaa agttggaaat gttaccacga gctccagaat
    25561 cagacatcat gaagattgga tttttcgctt ccaaaccttc catcagtttt tgttggatgc
    25621 tgtcttttgc agcattccaa acagcaatga cacgttcata acgttcgtca tctgtgatca
    25681 gaccacgacg gaattgtttt gtaatagttt caacttgttt atgggcattt tcaatgattt
    25741 cttgtttttc attcagtaca ctgatgtcag cgatccctac tgtgataccg gcatgtgtcg
    25801 aatgtttgta ccctaagtct ttcatacggt caagcatctt agaagtttct tttactttga
    25861 atcgtttgaa gacttctgcg atgatgtttc ctaagttttt cttcttgaac ggtaatacta
    25921 attcttgctc tttgatatgt gcaggaatat cagtaccagc ttcaacaaag tatttgtccg
    25981 gtgtttggac ggtcaagttg aagtctgttg gttcattcag ataagggaat tcaggaggca
    26041 tgatctcgtt gaagatgatc ttaccaactg ttgtgatcat cgtacgttct ttttgccatt
    26101 cagtaaatgg tttttcacct aatgagttag ggttcacacc gatacgtgaa tgtaaatgaa
    26161 catatccgtt gcgccatgcg ataacggctt cgttttgatc tctgaagacc attccttcgc
    26221 cttcgcgtcc ttcttcttcc attgtcaagt agtagttacc tagaaccata tcttgagaag
    26281 gagtaacaac tggtttacca tctttagggt tcaagatgtt ttgagcagct aacatcaaca
    26341 tacgtgcttc tgcttgtgct tcttcattca atggaacgtg gacagccatt tggtctccat
    26401 cgaaatcggc attataagct tcacatacaa gtggatgcaa acggattgcg cgtccttgta
    26461 ctaaaactgg ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc cggttcagca
    26521 atactgggtg ttccttgatt acgtcttcta aaacatccca tacttcgtct tcttgacgtt
    26581 cgattttacg tttcgcattt ttgatgtttg aagctagttc gcgttgaaca agctcacgca
    26641 tcacgaatgg tttgaataat tcaatggcca tttcttttgg aagtccacat tggtacatct
    26701 tcaagaaagg tccaacaacg ataacggaac gacctgagta gtcgacacgt ttacctagca
    26761 agttttgacg gaaacgacct tgtttccctt tcaacatatg agaaagagat ttcaatgggc
    26821 ggttacctgg tccagtaact ggacgtccgc gacgaccgtt atcgatcaat gcatctactg
    26881 cttcttgcag catacgtttt tcattttgaa cgatgattcc aggtgcattc aagtctaaca
    26941 aacgtttcaa acggttatta cggttgatta cacgacggta aagatcattc aagtcacttg
    27001 ttgcaaaacg tccaccttct aattgaacca ttgggcgtaa atctggtgga ataacaggga
    27061 taacgtccat aaccatccag cttggttgat ttcctgaagc gcggaatgct tctaaaatat
    27121 ccaaacggcg gattgcacgt gtacgttttt gtccttgtgc agatttcaat tcttctttca
    27181 attcggctgc ttctgcatct agatcaacat tatcaagtaa ttgtttaatc gcttctgcac
    27241 ccatagcagc ttggaattct tgtccatatt gttcacgttt ttcgcgatat tcgcgttctg
    27301 tcaacaattg ttttttctct aaattcgtat ttcctggttc gatgactaca tatgaagcaa
    27361 aataaatgat ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc atacgtgaag
    27421 gaatcccttt aaagtaccag atatgtgaaa caggagctgc taattcaata tgtcccatgc
    27481 gttcacggcg aactttcgaa cgagtaactt ccactccgca tcgatcacaa acgattcctt
    27541 tataacggat acgtttgtat tttccgcagg cacattccca gtcttttgta ggaccaaaaa
    27601 tccgctcaca gaacaaccct tcgcgttcag gttttaaggt acgatagtta atcgtctcag
    27661 gtttttttac ttcaccgtaa gaccagcttc tgattttttc gggagaagcc aaacctattt
    27721 gcatactttc gaatttattt acatcgatca aaaggggttc cctccatttc atttaatggc
    27781 aaaccgcaag acggctgatc ttcgaccgcc ttgcagctca tagaccggat tagtctaaat
    27841 tatcttctgc ggtttcaaag ttttggatga catcgtcttt ttcatcttcc acttgttcag
    27901 cagctttctt ttctaattct ttggcagttt gctgttcggc aaattttgtc aaagcatcaa
    27961 cagtgatcaa atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct gtttcttcga
    28021 tatccaagac acgcatgtcc agacctagtg attgcaattc tttcaccaat acgcggaatg
    28081 attccggtac acctggtttt ggaattggtt cacccttgac gattgcttcg tatgttttca
    28141 cacgtccgac tacgtcatct gatttgtatg tcaagatttc ttgtaaagta taagccgcac
    28201 cgtaagcttc cagtgcccat acttccattt caccaaaacg ttgtccacca aattgagctt
    28261 tacctcccaa tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt gcatgcaatt
    28321 tgtcatcgac catgtgagca agtttgatca tgtacatcac accgacagac acacgtccat
    28381 caaatggttc acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg gccataccgg
    28441 cttcacgaac tgtttcccat acgtcttcat cacttgcacc atcaaatact ggcgttgcta
    28501 cgtggatacc taactgacgt gcagccatac ccaagtgtaa ttccaatact tgcccgatgt
    28561 tcatacgtga aggcacccct aaaggattca gcatgatatc gattggtgtt ccatcaggta
    28621 ggaatggcat atcttcttcc ggcataatac gggatacaac ccctttattt ccgtgacgac
    28681 cggccatttt atccccttca tggattttac gtttttgaac gatataaaca cgaactaaca
    28741 tgtttacgcc tggtgacaat tcatctccag cttcacgagt aaagattttc acatcgtgaa
    28801 cgataccgcc gccgccatgt ggtacacgta atgatgtatc gcggacttca cgagcttttt
    28861 cgccaaagat cgcatgcaat agacgttctt ctgcagataa ttctgttacc ccttttggcg
    28921 tgactttccc tacaagcaaa tcgccatctt ggacttctgc accaatacgg atgataccca
    28981 tttcgtctaa atcttttaat gcgtcttccc cgacattagg gatttcgcgt gtgatttctt
    29041 caggtcctaa ttttgtatcg cgggcttctg attcatattc ttcaatatgg atagaagtgt
    29101 atacgtcatc ttttaccaaa cgacggctca tgatgatcgc atcttcatag ttgtaccctt
    29161 cccaagtcat gaaaccaact aaaacgtttt ggcctagagc catttcgcct tgttccatag
    29221 atggtccgtc tgccagagta tccccttttt cgactttctc acccaaatga acgattggac
    29281 gttggttgta gcttgttccg gagtttgaac gacggaattt tgttacatcg tatttatcta
    29341 atgcaccgtt gtcgcggcgt acgcggattt cagaagcatc aacgtattca acgacaccat
    29401 catgtttaca taacaaagca gctcctgagt catgcgctga tttgtattcc ataccagtac
    29461 caacccatgg tgcttgagga ttgatcaacg gcactgcttg acgctgcatg ttggcaccca
    29521 tcaaggcacg gttggagtca tcgttttcca agaaaggaat acatgctgtc gcaacggcaa
    29581 ctacttgttt aggtgatacg tccatgtagt ctactttatc aatagatact tctaagtttt
    29641 cactttgagc acgagccata acaacgtctt gagcaaatgt tccatcttca ttcaatgggc
    29701 tgtttgcttg ggctacgatg taatggtctt cgatatcagc tgtcaagtag tcgatttggt
    29761 cagtaacacg acctgtttca cggtctacac gacgataagg tgtttcgata aatccgaatt
    29821 tatttacttt cgcataactg gacaaactat tgatcaaccc gatgtttggg ccttcaggag
    29881 tttcgatcgg acacatacgg ccatagtggg agtagtgaac gtcacgaact tcataaccgg
    29941 cgcggtcacg agtcaatccg ccaggtccta aggcgaaaag acgacgtttg tgtgtcaatt
    30001 cgccaagagg gtttgtttga tccatgaact gtgacaactg agaagaacca aagaattctt
    30061 tgatacttgc cacaactgga cggatattga tcaattgttg aggtgtcaat gtttcagtat
    30121 cttggatcga cattctttca cgaaccacac gttccatacg tgctagcccg atacggaatt
    30181 ggttttgtaa aagttcaccg actgaacgga tacgacgatt tcctaagtgg tcgatatcat
    30241 cgacattgcc gataccttcc atcaagttca agaaatagct cattgaagca atgatgtcgg
    30301 ctggacgaac cgtttttaca gctgcttctg gatagccgtt tccgatcacg tttacttcac
    30361 gttcagggtc tcttggtgaa aatactttga tgacttggac agtcattgga tctgtgacca
    30421 caccgtcttc tgatggatag taagtcactg aatttaggcc attatcaatg aaaggagcta
    30481 gtgtttccat gacttggtga gtcagaactg ttcctttttc aacgatgatc tcgcctgttt
    30541 ctggatcaac taatgtttca gctaatgtca gattcaatag acgtgttttc aaatctaatt
    30601 ttttgtttac tttgtaacga ccaacgtttg ctaagtcgta acgttttgga tcgaagaaac
    30661 gagcattcag caagttacgt gagctgtctg ctgtttttgg ttcgcctgga cgcaaacgtt
    30721 cgtaaacgtc tttcaaacct tcttctgttc ttgagtcgct tgcgttttta tgcaagtctt
    30781 tttcgactgt attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc gtatcatcgg
    30841 aaccaaatcc taacgcacga actaatacgg ttaaaggaat cttacgtgta cggtcgatac
    30901 gtacataaga gatgtctttt gcatcagttt ccatttctag ccaagctcca cggttaggga
    30961 taacagtaga accgaatcct tcttttccgt ttttgtcgac ttttccatgg aaatagacac
    31021 ctggtgaacg gactaactga gaaacgatta ctcgctctgc cccgttgatg atgaatgttc
    31081 cttgttctgt cattaatggg aaatcaccga agaatacttc ttgtgctttg atttcgccag
    31141 tctcgcggtt tgtcaaacgc aatgttacat gtaatggtgc agagtagttt gcatcatgcg
    31201 cacgagcttc tgctactgta tactttggtt ctttcaattc ataatctaca aattccaaag
    31261 atagattgcc attgaagtca tcgattggca aaatgtcttc aaacatttct ctaagtccct
    31321 catctaagaa ccattggtaa gagtctgttt gaatttcgat caaatttggt aattccaaca
    31381 cttcactgat tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt actacgtgtc
    31441 cagccaagct cttcacccct ctaaaatgtc attcaaaaag actacccgat caattgttac
    31501 aataatattt caaatattat ttttaggtaa caacagacat tttttgcggc ttttaggcaa
    31561 aaaaaaacca aaaatagaac attctcttga aaccttactt cgtgagcttc tacttttgtt
    31621 tccttatatt aaagccgaaa cggacaatat ttcgtttctg ccttttcttc atcagatagt
    31681 ttttgcaact tcatatacta cacttatttt ctataaaagt caacacttcc tggcgtttct
    31741 tctgcttttt cgcaacattt tcttaatgtt ttcaatttta tgttttcaat tatctgacaa
    31801 ttcttaattg acagaccttt ttatctcggt tatactttag aaaattcatt ttgtggagga
    31861 acttctatgc gaaccaacat caaccaaatc gcaccaacac aactgaataa cttttacttt
    31921 agctacttta gataggtttg tattcatcat gatggataca aacgttaaac gacagtttgt
    31981 atctatgatg gctaaagaac tttgtattgc agcaaaaagc cacgtagaca aaatgctaag
    32041 tggctgagca gtgaaaatac aaagtaaaag tatcaagcat tctacttttt caatttattt
    32101 tccgactttt caatccattt ggacattttg tctgaatgga ttttttgttt cccaaaatta
    32161 aggaggaaaa agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc ctgctactag
    32221 ctttaggagg ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga caaaaaacaa
    32281 ttggcgtatt gcaacttgta caacataact cgttagatgc tgcctataaa ggattcaaag
    32341 aaggattagc agaagaaggg tataaagaag gcgataatct aacaatcgat tatcaaaatg
    32401 cccaaaacaa tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa gaagatcctg
    32461 atttgctact tggaatcgcc actccagcag cacaatcttt attaaatgaa acaaccacta
    32521 tcccgatgac tgtcacagcg gtcacagact tggaacaagc ccatttagtc acatccaatg
    32581 aaaaacctgg cggcaatgtg actggcacga cagatatggt gccaatcgaa aaacaaatcg
    32641 gcttactgtt aaatatcgtg ccagacgcca aaacaatcgg catcatgtat aacaacggtg
    32701 aagcaaactc taagatccaa gctgatttgg cagaaaaaga attgaaaaaa gcgggcgtca
    32761 aagtcaaagt actgacagca aattccacaa atgatgtcca acaagtgacg aaaagtctag
    32821 ctaaagatgt agatggaatc tatattccta ctgataacac gttcgcaagt gcggcaagtg
    32881 ttgtcggtga agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca gttgatcaag
    32941 tcaaagacgg cggtctagca acagtaggaa tcgactacga aagcttgggc aaacaaacag
    33001 ggatcatggc agcaaaaatc ttagacggca agaatccaag tgaacttcct gtcgaaacag
    33061 cgaatgattt ggaactgtat gtcaataaag acatggccaa agcattaggc atcaatccag
    33121 acagcatcaa agaagctgaa taaaacacag gaggattctc atgacaaatt tgctctctct
    33181 tttcttatca tctgcatcgc aaggggtctt atgggcccta cttgcgattg gtgtatacgt
    33241 cactttccgt atcttggaca ttgccgactt gactgctgaa ggaagctttc ctttaggtgc
    33301 agggatcgcg gcagtaagta ttacaaatgg ctatcatcca ttgacagctt gtcttctagg
    33361 ctttttaggg agtgcggcag cagggcttgt ttccggactg cttcacacaa aattgaaaat
    33421 tcccgccctt cttgcaggga tcatcaccat gactggttta tattctgtca catcccgtgt
    33481 gatgggtgcg ccgaacatct ctttattggg tcagccaacg atctttcatt gggtcgagtc
    33541 attcggcata acaaaagaca acgctgtact gatcgtcggt ctgctttttg ctttgatcgt
    33601 tgtatgttta cttgttttgt tcttacgtac agaaacagga cttgctattc gtgcaactgg
    33661 cgataatctg gcaatgagcg aagcaaacgg gatcaacaca gataatatga agatcatcgg
    33721 ttatatgatt tctaatggat gcattgctct ttctggctcc ttgcttgcgc aaaacaacgg
    33781 ctttgctgat ttaaattctg gtattggaac gatcgtaatt ggacttgctt ctattatcat
    33841 cgcagaagtt ttgttacgta accagccact atttctccgt ctcttgactg tcgttttagg
    33901 tgcggtcatt tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg aaccaagtga
    33961 ttcgaaactt gcttctgctt tcgtgctagt gatctgtctt tctttcccgc agatccagca
    34021 aaaattccat ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga cgtcttaaca
    34081 ttggaaaatc tccatcatac ctttgaaaaa ggaacgatca acgaaaatca tgtcctgaaa
    34141 ggcattaatc tttccttaga tcccggcgac ttcgtgacga tcatcggtgg aaatggggct
    34201 ggtaaatcta ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca gggtcaaatt
    34261 ttattgaatg gaaaaaatat cacgaaaaaa tctgttgtcg aacgttcgaa atccatcagt
    34321 cgcgtattcc aagatcctaa actagggaca gccgttcgtt taacggtaga agaaaatctt
    34381 gctcttgcta tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa accacaagac
    34441 cgaacatttt tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa tcgtttatcc
    34501 tcagaaatcg gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt gatggccaca
    34561 ttacagcgtc cagaactgat cttattagat gaacacactg cagcgcttga ccctgctact
    34621 tccaaaacgg tgatggaatt gacagaaaag ctgatcactg agcagaatct cactgctttt
    34681 atggtcactc atgacatgga agacgcgatc cgctatggga gtcgactgat catgctgcat
    34741 caaggaagga tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac agtgccccaa
    34801 ttattagcgt tgttcaaaca aaacagcggc acggcattaa aagacgatca agtactttta
    34861 tcttcataat cagaaagagg atgggacaaa aaatgaacgc ccaccctctt cttttttctc
    34921 tttccttctt tcgtttttgc tttatgtatc aaccgttgtt tcttcgaaaa ctattttgtt
    34981 ctctccttct tcctgataga atggatggga aaggagcttg atgccatgac aacgaaagac
    35041 aaggtacttc aattattgaa aaagacacca gattttcttt ccggagaaaa gttggcacag
    35101 caactggaag tttcccggac aagtatttgg aaagccatca aagaattgga aaaggcaggc
    35161 tatcgttttg agcacgggcc tacagggtat cgttacctgc cctctgacgt actagatgca
    35221 aaagaaatcc agcaagagct acggtcgttt attcctgatt tgaaagtaca aacgatgatg
    35281 acttctgaat ctacgatgaa agatgccaaa ctggcagcag ctcaaggaga ttctgtttcg
    35341 aagttatttg ttgctgatac acaagaacaa gctcgtggac gttttggacg gccttttttc
    35401 gctgaacctg gacgaggaat ctacatgagt ctcttgctcc atccagacaa acggtttgat
    35461 gaattgcctc aatatacgat catcgcagcc agtgcctgtg tgaaagccat tgaatctgta
    35521 accggtcgtt ctgcttcgat caaatgggtc aatgatatct acatggatgg aaaaaaagtc
    35581 tgtgggattt tatctgaagc agtcagtgat atggaaagcg gcaggatcag tcatatcatc
    35641 attgggatcg ggcttaattt ttctgttcct caaaatcatt ttccagctga tctccaagaa
    35701 aaagccatct ctgtctttcc cgacggacaa gctagtgtca caaggaatca gctaatcagc
    35761 aaaatctggc aagaattgtt cacttatctt tctcacccag aaaaagcgtt ggacttctat
    35821 aaagaaaaat cattcgtatt gggaaaaacc gtgtcgttca atcaaaaagg aacgacttat
    35881 gtcggtcttg caaaagaaat cggtgatcat ggcgaactga tcgtccaaac aaatgaacag
    35941 aagatgagtt tgtcttctgg cgaaatcagt ctcacatcta tccgtacgca ataattatca
    36001 gatttcacaa aaggacacct gatctcatac agtgtccttt tttctgaatt ctcttcaaaa
    36061 gactctcctt attctatcag ctgacagttt ctctatcctc tagaaaaagg tattctaaaa
    36121 atatctataa agcatggtac actttcttta aatgaatcaa tgcaaaaaac aagagagtca
    36181 tcagtttaca cgatcaaaag agatagcagg ccgcttcctt tttcaatctt atagatagac
    36241 catgggtaac gataaaaaca aaaaagaact tgcgacttat tttgccgtaa gttctttttt
    36301 agctagtgcc ttaatctctt aatcccaaca aacgtctcgc atagatcacc tgaccagagt
    36361 gcatagcagc atcatcaata atagaaacga gacgcattcc tcgtgtgact ggtggtgtcc
    36421 agttctcgtc aattacttca tctagctcgg aaggttccag ccttcttaag taagaaatag
    36481 caaaatcagc agctcctcgc aagtatccta gcagatcttc tatttgatta gcttggatct
    36541 gctttgcttg ttccagacta tgcttccaat cttgttcgtt atctgctatc gtcatcggaa
    36601 accggctttc ccagtcttga ctatgccaaa ttggagaagt tcctgctaaa tcagcaatct
    36661 ggaaatctaa ttctcgagcg gtatgccaag ccagccaagt cattgatttg atctgcgggg
    36721 ctctatcttc cactggaaaa cgattggcct cctcaataga gacgccttca aacaaacgtt
    36781 caaaccgatc gtatgcccga tccacactct cgcatagcat atctaaataa tccatttatt
    36841 ttcctccttt ttatttatcc tacttacttt ttcctaattc taggatacct acttatatgt
    36901 tactacacac ccttattttt tacactttaa cagtcaccat catcaatagt ttggagattg
    36961 aaagaaaaag atagtgtcga actatcaatt gaacaagaag caacaatatg aacgtttcat
    37021 taatattctt taaataaaca atgatttttc aaacgaacag tttaagacta ggagaaaaag
    37081 agtataatag gaaaatgttt gaaaggaaag gaaggggaaa aatgaaaaaa agggcaagtt
    37141 tactagtcct agttgctttg ttgttcaata tcttttcgta tggtgtggcc gtttcagcgg
    37201 aaacatacat tcctaaagac cctgggaata tttataatga tctaactggt aactatggct
    37261 tgttaggaat tgctagtcaa ttccacgtat ttgctaaaaa taagacaact gtgaatgcac
    37321 atacagacgg aaatatagca acacatgaac ttgatgcaaa aaataatttt ggtacagagc
    37381 ttcatgaagg tttattgaac caagaaataa attatataca aaaaactgat tctttcgcta
    37441 attcttcagg catacctaca ggtagcacaa gaatgaataa atttgtcgtt ggcagtaacg
    37501 tggatgtagc acttcaagat ggtcatccta cagtcaatgg atccaggctt gatcacttaa
    37561 ctccagatga tttttataaa gatcgtgctg ggcatgaata tattgatttc gagcaggaat
    37621 ttgctaaatt gaatgtagca gcaaatgatc ttgcaacgat cactccagct aaaacatata
    37681 ctgctgcaga ttttccagac atgaacaatc ggacaatcga tttaactgat ttatcagata
    37741 ccgggttttt attggtaaat atcgatgcag aagtgttaac gatgaatacg ccacttcaaa
    37801 tcattaatcc aaatgatcaa gtagttgtat ttaatgtcat caacagttct tctgcattga
    37861 acgtacagtc accaatcaaa tacaatgatc gttcaaatca tgaaacagaa gatttttcgg
    37921 atgcgaatat ctcttggaat tttggaaatg agatgactga tttgacaatc agtgctcctt
    37981 ttcaaggaac aatccttgcg cctaatgcta ctatccgcat tactcaaaac caagacggaa
    38041 cgattatagg aacaaatgtg attttagatg cagctaccaa tcgttgggat cctaacgaaa
    38101 tatttatcag cgacaatggt acagatacga cagacacaag cgacagtaca gatacatcta
    38161 ccacaagtga tagctcagat tcatcaactt caagcgacag tacagatact acctctacca
    38221 caagtgatag cacggataca tcagcttcaa gcgacagtac agatactacc tctaccacaa
    38281 gtgatagcac ggacacatca gcttcaagtg acagtacaga tactacctct accacaagtg
    38341 atagcacgga cacgtcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    38401 gctcagattc atctaccact acaagtgata gcacggacac gtcagcttca agtgacagta
    38461 cagatacatc ctctaccaca agtgatagct cagattcatc aacttcaagc gatagtacag
    38521 acacgtcgtc taccacaagc gatacacgtc atgcaagtgt ctccttctca aaaacaagtt
    38581 cggataacag gacgaacttt aacttaacta ataaaacaag caatgacaat ggaaacaaat
    38641 caaaaagagc acttcctaaa actggaagtc aatcgaataa ttggataaca ctcgcaggcg
    38701 ttatattact tgtaatcggt ttaagaatca cttttatgag ttataagaaa tcgaaaaggt
    38761 aaaaaaatat tcaagataaa aaagagcaac gtcgctaacg gacgttgctc tttatttttg
    38821 ctttttatca taaatacaag aactacaatt caatttgtca aaaaacacgt agatttcctt
    38881 ctgagaaatt tatttacgta tcgaaccttt ccctattcag tctttgcgat ccaaaatcag
    38941 ttgggtaagg gaaaagagat tctcttttgt tcgaagagag gtggcaggta atttctttat
    39001 ttccttcaaa gccttttctg tgtaagaggc cgctaacttc ctagcttctt ccacgcctcc
    39061 tgtatgttca acgattttgc gtacggcgtc gacatcttct tctgtcattg ccgctttctt
    39121 ctctaaataa ggtagtagct ttggtttgtt tttttgcaaa gagtagatca acggtaaaga
    39181 atagacccct tgacgcatgt cttctagtac aggtttgccg atttcctggc tctcttttgt
    39241 ataatctaaa atatcatcga taatctgaaa ggccatcccg atagaaagtc cgatatctcc
    39301 tgcttttttt gcgaagcgct cgcttgttcc actttcaaaa gcgccaacag aacaacttaa
    39361 ttggaacagt tcagctgtct tacctgaaat attttccaaa tattctttta cagaaagttc
    39421 ataattatag cgatcatcca tctgccctaa ttcgccattc aatacttttt ccatactgcg
    39481 agaattcaat tggagacttt tcaaggaagt ggagtaatca gacaatagtt taaaacagca
    39541 gacaaataaa tagtctcctg catagacaga aacgacatta ccaaactgac tacggatatt
    39601 aggctgacca cgtcgcagat ccgcttcatc aacgatatca tcatggatca gtgtggccgt
    39661 atggagcatt tcgatagaag ctgccaaagc gatggctttg tctcgatctt gctctggtcc
    39721 aaattgtgag aacaatagct gataggcagg acgcaacagc tttccgccgg catggatcgt
    39781 ttctttgatt gcttgttcga caggtttgtt tttcaggcgg acagtttctt ccatccgttt
    39841 tagtgtttct gtcaattctt tacttaaagc aggatagttt tcccacattg gatgtagctt
    39901 cataaaaaaa ctccttgtgt gacgattatt ttttggtaaa cattgggctc catttttgtt
    39961 tggttccctg ctcataatct ttttggaatc gttgaagtgt ctgcacatgt cgtaaaagat
    40021 aattcgctgc gatcccgatt gcaatccctg ataaaatccc taaaactgaa aggactggca
    40081 agtacaacat gactgtccat gattgtgcaa tgaaagaagc catcaaaagc tgtccgacat
    40141 tatgcatgaa accgccggcc gcactaattc cgatgatact gacacgtttt ggtcctaatt
    40201 gcttgaccaa taacattcca aaataactaa gcaaagcacc actcatacta taaaggaatg
    40261 tggaaatcgt tccgcctagc aaagtagtca ggaatagtcg tagccaaagt agtaagaagc
    40321 tgtcttgttt tttcatggta aaaatggcaa cgatcgtgat tagattggct aagcctagtt
    40381 ttgcaccagg cgcaaaagaa aaaggataag ggatcatatt ttccaataaa ccaatgatca
    40441 ctccttgtgc aacaagtagc gaaatataaa tcattttttg taatttactc atggtgtgta
    40501 aaggtctaat aaatcagact gtcttcgtca cctccatctg ttgaacggac ttcgatcact
    40561 aatttatgtg gcaaacagac gatggtttcg ccatttttcg ttgcccagcc tcgacgtacg
    40621 cagatctggt catcgcaatc tgcttctttt atccgtactc gatcgccatc tatctcgatc
    40681 aagttataat ctccgtgttc atctttgtaa gtatatgtgt tgctctcttc tccagccatc
    40741 aaagggaatg ttttgatttc tgtgccatcc acacgcagta ccgcttcttt ttctgctgtt
    40801 gcggtgtttt gctgaagacc aaaaatcatg actggtaaaa aagaaagaac agtcaaaagc
    40861 aaaataataa aaacatccca aggtttgagc agactttttt cagcaaattc tttaaggctc
    40921 atttttgttc tcctttttcc agattaactc acccaatttt agcataaaaa caagaggttg
    40981 tgacacctgt gtcttgcttt gagtaataag aacgaagaat agaaaacagc tttccatgtt
    41041 ttctaattct ttgggcttat taccgagaag cagcaggtgg aacaccgttt ataacaagag
    41101 gttgtgacag aagtgggcaa cttcaagaaa taagaaggaa tttacgaaaa ttacttttca
    41161 agtttttgtg aaatttcggc ttatttttcg aagaagctac ttctggaaca ccgtttataa
    41221 caagaggttg taacagaggt tgtgacacct gtgtcttgct catggaggct tatctcgggc
    41281 atcttattcc tttaatttta cataaaaagg aggctgtgac ggtttgtctc agcccctttc
    41341 tattgaaatc ttgcattcaa ataacgttct ttatctattt atttcttgcc gtactgggaa
    41401 cttggtctac cgtaccacca attacctagt gtacgttgta gccatggaat tacccagcga
    41461 tccaacccaa tcgcacgtcc ggaaccgttc atcaatgcga aagcaacaaa aatgaaccaa
    41521 atgtttaccc agtaaaacat gcctgataag cagaacgtga tcaccaatac aatcgttgtc
    41581 gcactgcata gccatgtgaa taatccagca atcaatgcta atgccaaggc aatttcaaag
    41641 aataccatga atttttggaa gaacattgct acatcattgt ttggcatgat cacttgcatg
    41701 atctttgtga accagtctgg tgacttctca atcacagcca ttggttcttc accgtatgca
    41761 taactcaaac caaaatgtgc agcttgagtc gctgcttctc cggcacttgc ccctgctccg
    41821 ctggcagctg tggttgcatc accagcagca gaagcaccac tggcaacatc tgttacattg
    41881 tattcccatg tccaagggaa cacactgttt ccagtgaacc atgatccttc accaaaccaa
    41941 gtacttggtt tgaacacttc accattccca atgatttttt tggaagcttc aacgaaccaa
    42001 actaaaccat agaaaatacg aagtggcaca ctccataaga cattgccgta acgagaagaa
    42061 tgtcctcgag tcacgttgcg atcatctttg atatggaaaa actcatgcat taaatattgg
    42121 aacatataat aaccagaacg aatatcaaag aagtatttca aattcacgat atgtttcatt
    42181 acgatagcta aaaatccgct taaatggatc ttatcaaata ggttagctac cccccatttg
    42241 gcaccaatag aaaccataaa tccttgatag tttcctttaa atttatgctt ttctccgcct
    42301 ttgatcgaag ccacgatatt cgctgcggct gtgtgtcctg tttgttcagc agcttgaacg
    42361 atctgcggtg tcggtgttcc tgggaattct tcataaaaga ctaaatcacc aatgatatag
    42421 atgtttttat cttcgtagcc ttttgcctgc atatattcat tggcgatcaa ccgctgtccg
    42481 cgtgctgatt ctaatccaaa gtcagcagcg tcagaagttc ctttgacacc agctgtccaa
    42541 atcagtgtgt gagtcggaat gttgcgtccg tctttcaatt tgatatgatc agcagccact
    42601 tctacgattg gtgcattcaa taataattcc acgttgtgct tacgcaaata gcgttcagct
    42661 tttgcagcat catttcgatc caacatattc aagatcgtcg gcattgcctc tacgaccatc
    42721 agtgtgattt cgtttggatc taatttataa tctttggcta ggcgattttt ccagtcgatc
    42781 agttccccaa tcatttcaat accggtaaat ccagaaccac agacaacgaa agtcagcatg
    42841 gctttacgca gttcatcatc tggttccaat gcagcttttg ctactgtctt ctcaatatgt
    42901 tcccgaatcc ttacagaatc ttcaaatgac caaagggtga atccgtgtgc tttgacacca
    42961 ggtgtaccaa agtcattcgg ctctccgccc attccaagga tcagctgatc aaaaggatag
    43021 gaacctaatt tggtcttgac gatttttcgt tctttatcga taccgattac agtgtctgtg
    43081 acaagttgta cattcttttg acgagcaaac aaatgctgca gatcgtattg gattgcttct
    43141 ggttctacac gtccgccagc tacttcatgc aactcggtca tcatagtatg gtacgaatga
    43201 cgatcgatca gagtaattgc tacatcttct tttttcagtt tctttgccaa gtattttgta
    43261 gctgaaacac cggcatatcc tgctcctaca acgacaatat tttgctttgc cattgatcat
    43321 ccgctcctta tagactatct atataatgaa tatatgataa ttcttttagt ttgatactta
    43381 ttacacaatc tccatatatt atatagatgt tctgttcttt tgtctaactc cttttggatt
    43441 tttttcaaat tttaaaataa agcgtttttt cacgaaattt gctgaaagaa aacgtttgaa
    43501 atattacatg ggttatttat caaaaatagt tgacttatag gctaaaaaga ttagaatgta
    43561 taatgaatca caaagaaaaa ttgtggacta gaaatggaat ggtggggtat taaaatgaag
    43621 aaaaaacgtt ttgtaactgg atttgccgta ttagcatttt cagctctagt gttaggcgct
    43681 tgcggcgcag atgacaacgg aagctcaaac tcttcttctg aatcatctac agcacaatct
    43741 tcgactgcaa aaagcacaac agaatcatct gcaaaagttg tagctggcgg agatttgcaa
    43801 gacggcacat ataaattaga agaaaaaaat tattcaaatg gttaccgtgc agtctttgaa
    43861 atggttgtaa aagacggcaa aatcactgaa tcaaaatatg ataacgtcaa tgaaaacggc
    43921 gaatcaaaaa caaaagatgc agaatacaac aaaaacatgg aagcaaaatc tggcacaaac
    43981 ccagaaaaat acattcctga gttgaatgac caattcttaa aagcacaaag cgcaagcggt
    44041 gtggaagtcg taactggagc tactcactca tctgaaagct tccaaaacta cgcacaacaa
    44101 ttgatccaag ctgctcaagc tggtaacaca gatacaatcg aaatcgacaa tggtgctgat
    44161 ttgaaagacg gcacttacaa gcttgaagaa aagaactact ctaacggcta ccgtgtgcaa
    44221 ttcgaaatga cagttgctgg cggcaaagta actgaatcaa actttgatta tatcgacaaa
    44281 gacggtaaat caaaacaaga tgatacagaa tataatgaaa atatgaaagc aaaaagcggt
    44341 acagaaccaa aaacttacat ccctactctg aacgacgaat tcgtcaaagc aatgggcgaa
    44401 gaagatggct cacctgctaa tgtagaagtt gttacaggtg ctacacacag ctcacattca
    44461 ttcatcatgt acgcacaaca attagtgaat gcagctgaaa aaggcgacac tcaaacaatc
    44521 gaagtagata atatcgtaac agaaaaataa tcacataatc atactaataa aagttggagg
    44581 gtgggacgat agccagatta ggcagtcgtc tcacctttca aaatgaagag aggaaaaaga
    44641 tgcggaaaaa actactattt tccttaatca cactgggcat ctcttttatg gtgctaactg
    44701 gctgcagcaa ctcagcaaat aaaacaacca ctgaaagttc agcagctacc aagatattga
    44761 aagatccata ttctgatgaa cagttcttgt tagggactta cgttcgtatc cgtgtgtatg
    44821 atgaaggcaa agaatcagca ttgaaacctg ccttcgatcg agtaaaagaa cttggtgata
    44881 aaatcacgat caatcaaaaa ggttctgaga tcgatgaagt gaatgagcaa gctgggatca
    44941 aaccagtaaa agtttctgat gacgtctaca cgttagtcaa gcgggcctac gaatatagcc
    45001 aagattctca aggcggattc gatatggcga ttggtgcaat cacacaatta tggcgaatcg
    45061 gttttgatga cgccagaaaa ccttcccaag aagaaatcga tcaggcattg aaattagtgg
    45121 attatcataa gatcgaacta aatgacaaag aaaaaactgt ttatctaaaa gaaaaaggaa
    45181 tgatcatcga tttaggtgca atcgccaaag gttacattac ggacgaagta gtgaaagtct
    45241 tgaaaaaaca aggagtgacc actgccatcg ttgacttggg cggtaatgtc tatgtcttag
    45301 gccacagccc aagaggcgaa aaccaagact ggacagtcgg catccaagat ccgaacatgg
    45361 ctcgcggatc tgttttaggt tctattaaag aacggaacaa aacattagtt acctctggaa
    45421 tttacgagcg ttatttagaa gtagatggca aaaaatacca tcatttgttt gatccaaaaa
    45481 cgggttatcc gttcgacaat gatatcgcaa gtgtcacgat cataacagat aaatcaattg
    45541 atggtgacgg gttgtctaca gcagtctttt caatgggtgt gaaaaaagga ctggaatacg
    45601 tcgaaagcga attgaataac gggacggaag cgatcttcgt gacaaaagac gataaagtat
    45661 atgtgaccga tccaatcaaa gacaccttca aattatcgga ggactcccat tacacgatgg
    45721 gtgaccgttc tgaactaaaa taaggagtac gaaccatgac actaaaaatc tttttagaag
    45781 tagttgaaat acgaacaaag gtggctagtg tatttccttt tgcggtaggg gtattattct
    45841 ctatcgcttt ttttcatgag atacattggc taaacacttt tttatttttt attggaatgc
    45901 tagtttttga tttagcgact accgctatca ataattacat ggattatgaa aaggctcatt
    45961 ctgaagtata taagtatgaa gaaaatgtca ttggccgcag aaatctatct cctgtattga
    46021 ttcgaaacat gattttagct atgattgcat ttgtttttgt tatcggcgtt attctaacga
    46081 tcaatacggg ttggctttta cttttgatgg gatttatctg ttgttttatt ggagtattct
    46141 atacattcgg accgattcct ttatctcgaa tgcctttagg ggaaattttc agtggggtga
    46201 ctatgggcct tggaattttt accatggtcg tttatgtcaa tacattcggt tcagatgtct
    46261 atgatctgac agtcaacttt tcatctggac aatttgcttt ggtaggaaat ctttgggcta
    46321 tagcagcaat cgtattagct tccttaccct tggtgtttac gattgccaat atcatgttgg
    46381 ctaataattt gcgggatctt gaccgtgata ttgaaaatca tcgctataca ctagtttttt
    46441 atatcggacg tcctgcggga acagtcttat tccagctatt gatgtatgct tgctatttcg
    46501 tgattttaat ggggttgttg agcggtatct atgaatggcc gatcttggtc accttcttga
    46561 ccttgccaat tgtgaagaag catttggatc aattcaagca aacgttaccc cagcctatca
    46621 gtttcagcta ttccatcaaa aatatggtct tattcaatgc cagttatgta ttggggctat
    46681 tactaacaat cattatgcaa gccatttaac agcatgaaga ggttgtgaaa aaactgccct
    46741 gcatcaagta ataagaacag ccaaataaaa atagctcctt atatttttcg tttggttggg
    46801 cttattaccg cagatgcaag cttttgaaca ccgtttatca ggaggttgtg aaaaagtcgc
    46861 ttagtttcga gtattaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
    46921 tggcttaata ccgagaaact ggcttttgaa caccgtttat cagaggttgt aaaatcagtg
    46981 ttttgtcctg agtattagag aaacaaatgg aaaaatagtt cctcatattt tgccgttttt
    47041 tgagctaata ccgaaggaca gctgattgaa cactatttgt tcggaaaaag tgactaacta
    47101 tgacacaatt tttttcacag tccctttttt cacttgattt gattcgtacc agcgaacttg
    47161 acgagaactt tcttggtttg gtaactatat ggtattcttt taacatcctc gtttgcttct
    47221 tctaatttat ttatacaaag tcacactttt ttaaacatct gttcttcgat acatttctat
    47281 ttcttttaaa catcgatacc aatattttga acttaagagt gcataccaaa tggattcatt
    47341 tactcttcgt cagttacgaa gataatcaaa atctccaact tccctactta tttcgttttg
    47401 agaattatcg aagcaatctt acataaataa ctaaaaaaca aaaaaccaga aaatcagtat
    47461 actgattttc tggttttaca tggtttgttc aataaaaagc tgttggaact gttaatatta
    47521 aattatcgca tcgttacgaa ttcttccgct cctgttggat ggatggctac tgtattgtca
    47581 aagtcttctt tagttgcacc catcttgata gccacggcaa aaccttgcag catctcatcg
    47641 acaccaacac caatagcatg taagccgacg attctttctt cttttccgac acagatcaat
    47701 ttcatttcgc atttttggcg ataatcattt aatgcaaagt acattggggt aaagcgtgaa
    47761 cgataaactt tcaattcatt ttcgccatat ttttccaatg cttcttcttc tgtcaaacca
    47821 attgttgcaa ttggtggatg cgtaaagacg actgtcggaa caagtttata gtcaagataa
    47881 gaatctttct ttccattaaa taaacgttct gataaacgtc taccagctgc gattgcaaca
    47941 ggagtcagat ctaatttacc gatcacatca ccaacagcaa aaatatgttc atccgttgta
    48001 ttttggtatt tatctacttt gataaaccct tttttgtcca attccacatt tgtattttcc
    48061 agacctaatt tttcggtatt cggtacacgt ccaccggcaa aaagtacaca atcaccagtt
    48121 aaggtttcac cattttcaaa tagcaccgta tattcgtcat tgtttttagt aatttcttta
    48181 gcaatatgat gtgcgtaagt ttttattcca gcttcttggt acatttggat caaattgtcc
    48241 gaaagcattt tatcaaacgt tctaagtggt cgttcttttc gatatgccca catgacttca
    48301 ctacctaaac cacgaaatac acctgaaagt tcagccgcaa tgtatccagc acctaaaacg
    48361 attgctctct taggtaactc tgttaattca aaaaatccat ttgaatcaat tgcgtattct
    48421 ccaccaggaa tggtcatttt acttgcacgg cctccagtag cgatcaagat tttaggagca
    48481 cgataggttt caccgtttac ttcgatcgtt tgactatcga taaaagtagc gtaacctttt
    48541 atagcctcga catgattgct atctaatcct ctttgataag caccgtgtag aaaatcaata
    48601 tatttttctc gtctttctac tagaccggcg aaatcaacat tttttaaatc tgcttggata
    48661 ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg tacttgcttg ccacataact
    48721 tttttaggta cacaaccgac gttgacacat gttccgccca attcattcgc ttcaatcagc
    48781 aaaacttttg cgccatgcat ccctgctctg tttgctgaag caataccgcc agagccgcca
    48841 ccaattacga tgtaatcata tatttgcatt ttgttcctcc ttattgaaga gatcaatcaa
    48901 aacctttatt taacgatcct ttttctacag aaaacaagtg taacactttt cttgaatttt
    48961 gtatgcgaag atgcttaaaa cagggatata tcagcttttt ccttatttac gttttcggat
    49021 catatctcca ggtttcaccg gctgtgcgac tggcatagtt aaaatcatca ttggatttgg
    49081 tgctcggtca atggattcgc cttcttcatt atgcataact tcaacggttt gactgaaatg
    49141 actgaatcct ggaccataaa attcgatttc atctcctaca gaaaacaagt ttctctgacg
    49201 tatcgtagca atctgtgttg cctcatcata agataatact tcaccgacaa atttatatac
    49261 tgggatttta cgacgttcac cgaataattg ttcgttttca gttggtgtat tgtagtaaaa
    49321 acctgtagct aattctcgtt gtgctacttt ccataactcg tcgatccatt cttgcttgca
    49381 gacatagttt tcaggatctt ccatataact gtctacagcc gctttataga catttgacac
    49441 cgtagataca taatggatcg atttcattct tccttcgatc ttgaagctgt ctacgccgtt
    49501 ttcaatcaat tctggaatat gttcgatcat tgacatatcc actgcactca tcgagaattc
    49561 ttcttccact tcgcctgtat cagttaaact gtttcgttct tgaccaaaag gcatatcgaa
    49621 caatccatac ttccagcgac aagattgaga acatccgcca cggtttgcat cccgcatcga
    49681 catatggttg gacaatgtgc atcgaccaga ataagagata cacatggcac catggataaa
    49741 tgcttcgatt tcggtatccg tatttttacg aatctctgcc acttcctcca ttgaaacttc
    49801 acgagctaaa acgacacgtt ccaacccttc ttctttccag aattccaatg tctcaaaatt
    49861 agttgctgaa gcttgagtgg ataaatggat cggcagacct ggggcttctg tcgcacaaat
    49921 ttcgatcaaa gctggatcag aaacaatcac tgcagaaata ccgacatcac gcaaactgcg
    49981 gaaaaattcc cctgctccat cttcatttcc ttcatgggtg accatattag cagcaacgta
    50041 taccttcgca tcatgttctt ttgcaaaagc aactccttca gccatttctt cataagtaaa
    50101 attacctgca cggcttctta aaccatatgc attcccgcca atatatactg catctgctcc
    50161 ataatggatc gcggttttta acttttccag tgttcctgct ggcgctagga tttctggtcg
    50221 tttcaatttt ctttttactg tcatttcttt catcatactc cttatttgat agcagaagga
    50281 tcaatataaa agaatcctgt atctaattca cggttttcag gatgcaattt actgatttcg
    50341 ctagtcattt tttccgcttt ttcttgatcc cagctacctt ctaaaatttg tttttttgct
    50401 tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg gagtaaataa tccgtccaat
    50461 ttccatgtac agtaatgatg ctgataaagt tcattcaatt cattcgagag attcagatca
    50521 ttgctggcaa aaatatgtgt gccatgacta tcttcataaa tagaataatg tgtctcttct
    50581 tttttaggtt ccgagataaa aagacctctt tcacgatctt tttgttcgtc ttgtttcgta
    50641 tagttatagt aattctgtag cagtggacgt ttggattgat ggatgcatgt agcaccatac
    50701 acaagggttt ctacaggaat atcgagtttt tcttccatcg ccttcatctc ttcaaaagga
    50761 acttcacgag caagtactgc tccaactgcg ccttttttcg cccagaaatt gatttgacgt
    50821 gcgctcgtta ctaatgtttc agcatcataa atatacggta tacgcaattc aggatttttt
    50881 ttcatcgtat aaataatacc tgtatcacca actgtgatca catctacgtt tatttctttt
    50941 aaaaaagcca aatattcagg tacatttttc attttttcag gatgaatgat cccatttaca
    51001 gcaatattcg cttttttacc atacttgtgt gctaattcta ccaattgtcg ctgttcttct
    51061 cgtgtaaatg aagcaggcag acgcaatcca aattgctctt caccgaaata tagtgtatct
    51121 gcacctgcag caagcaactg ttctgcttgt tcaatagttt ctactgtggt tatgagttcg
    51181 atcataattt tctcctccta caacttcata atagtagcat agctctaaaa actttccaat
    51241 acttcttcta tttaaagtcg gatttttgaa aattatttca caaaaaatca aaataaagtt
    51301 cgatatattc tgtccaaata taaaaagaga ttgtgacaaa aatcttgtca caacctctta
    51361 atcaagtctt actattataa aaattcagct acacaggcca agtaactata gggaacctac
    51421 tgtttccttt ttaggtccgc gaacattcaa tgtgatctta ccttttgaag cacctactgt
    51481 tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc tcgctcaatc gatcttcaat
    51541 ttctttttga agtgcgcgac ggattggacg tgcaccatat tcaggatcaa atccagcttt
    51601 cccgatcaca tcgattgcag atggtgtgat tttcacttga atatcttgtt ctttcaatcg
    51661 tttgataatc gctttgctca tgatttggac gattgtatgg atttcatctt tactcaatga
    51721 atggaagacg acagtctcat cgattcgatt caagaattca ggtcggaaag ctttctttaa
    51781 ctcttctaag atacgttttt gcatggcttt gtgatcttta gtaacatctt ggacattgaa
    51841 tccgacattt ttttcttctc gaatttgtgt cgctccgata tttgaagtca taatcatgat
    51901 tgtatttcta aagtccactt ttctaccttt agaatctgtt aaatgaccat catccaatac
    51961 ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc acttcatcta agagaatcac
    52021 tgaatatggt ttttgacgaa ttttttctgt caactgtcca ccttcatcat accctacgta
    52081 tcctggagga gacccgatta atcggcttgt actgtatttt tccataaatt ctgacatatc
    52141 aacacggatc agggcatctt cacttccaaa catgacttct gaaagtgctt tggccaattc
    52201 tgtttttccg acaccagttg gtccaaggaa catgaatgaa ccaattggac gatcaggatc
    52261 tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact gctttgacag cctcatcttg
    52321 accaacgaca cgttcatgca gaagtccttc cagttctaac aagcgttcgc tttctttttt
    52381 ctccaactgc tgtagaggga caccagtcca ctccgataca actgtagcaa catcttcttc
    52441 tgttacacga tcagaataac cttgctcctg ttttgcttca attgcagaaa ctttttgcag
    52501 ctttttacgt agtgcttttt cttgtctgcg taattgagca gcattttcaa agtcttgttt
    52561 ttggatcgct gcttcctttt cttgtactag ttcatcgatt tctagtttga tcacagttga
    52621 ttttgttaag tgatcggttt gatccaaacg gactttcgct gctgattcat cgatcaaatc
    52681 aattgcttta tcgggtagtt gacgagaagt gatataacga acagaaagat tgactgctgc
    52741 tcgtacagct tcatctgtga tctccactcc atggtgctct tcatatcttg tacgtaatcc
    52801 ttgaagaatc acttctgctt cttcaggagt tggctcatct acttgaatgc gagcaaaacg
    52861 tcgctctaat gcagaatctt tttcgatata tttttgatat tcatctaggg tcgttgctcc
    52921 gatcgtttgt aattctcctc gagctaacgc cggtttcaag atattcgaag catcaattgc
    52981 tccttctgct ccacctgcac caattaaggt atgcaattca tcaatgaata agattacttg
    53041 accatcatta tagatttcgt cgatgacctt tttcattcga tcttcaaatt caccgcgata
    53101 tttcgtacca gcaaccaaag atcccatatc aagcatcatc aggcgctttt cttgcatgtc
    53161 ttctggcact tcacctaaaa tgattttttg agctaaccct tcagcaatgg ctgttttgcc
    53221 tacaccaggt tcacccacca gtacagggtt attctttgtc cgtcggctaa gaatctggat
    53281 aagccgtttc acttctctcg aacgaccaac aactgggtcc agcttgtttt cttttgctaa
    53341 tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt ccttgcggtg tttgccgttt
    53401 atcttgtaca tttacatttc tacgacgatt cattcctttt ccagcagaat caccagctag
    53461 ccccattttt tgtttcagaa cttgacgggc ttttgaaagg ctgataccta agttgatcat
    53521 gattcgagta gccatgatac tttcatcttt taataagctc atcaaaatat gttctgttcc
    53581 aactttttga gaaccagttt tcttcacttc gctgctagct aaagcaaaaa cttgtttcat
    53641 tcttggtgaa tatggaagaa agactccttt agggtaagaa cggataccac cataaccaat
    53701 cagatgttca atttcttcat aaatttccgt ttcattcaaa tccatttcac gtaatacttt
    53761 tccagcaatt ccattttgct cgaccactaa ggctagtaaa acatgttctg aactaactga
    53821 ttgatgtttc aaccgttttg cttcttcttg tgcaatttct aatactgctt gggctctttc
    53881 agtaaaaagt tcttccatct aatctcactt cccatcttca taacttaaac gttccagaaa
    53941 tgctcggaga atccgagcac gtgtatgatc ttcatattca ttgaaattca atgtgttttt
    54001 tgcaattgca ctcaacatca agtttccttc tttttttgta attatttggt cttcataaag
    54061 tttttgaata atagcaaaag catttttctc actgattgta tcatcaaata actgatttat
    54121 ctgctcaagt aattgttttt tatctgaaat ccttactttt gcaattcgta tataaccacc
    54181 accaccacgt ttgctttcta ctagataacc acgttggatg gtaaaacggg tattaatcac
    54241 atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc atttcaatcc ttcgaatctc
    54301 gatcatttca ctattttcca gaattttctt tatatatgat tcaattaaat ccgaggtatt
    54361 ctgatgtgcc atcttattca ttccctttga ctaatattga ccttaattat aacgaatttg
    54421 ataatagaaa gaaagaagaa cgctttttgt taaataaatc ctttagaatc aacaaaaaaa
    54481 aatacggaat atactattcc gtatc
//
LOCUS       DAYYBG010000008        54453 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000008 DAYYBG010000000
VERSION     DAYYBG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.008"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACHAUN_000471"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACHAUN_000471"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8459201.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACHAUN_000472"
     CDS             complement(1034..1954)
                     /locus_tag="ACHAUN_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8459202.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACHAUN_000473"
     CDS             2067..2819
                     /locus_tag="ACHAUN_000473"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8459203.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3683..4216
                     /locus_tag="ACHAUN_000474"
     CDS             3683..4216
                     /locus_tag="ACHAUN_000474"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8459204.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4706..5230)
                     /locus_tag="ACHAUN_000475"
     CDS             complement(4706..5230)
                     /locus_tag="ACHAUN_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8459205.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5223..5696)
                     /gene="tsaE"
                     /locus_tag="ACHAUN_000476"
     CDS             complement(5223..5696)
                     /gene="tsaE"
                     /locus_tag="ACHAUN_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8459206.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5819..6799)
                     /gene="pta"
                     /locus_tag="ACHAUN_000477"
     CDS             complement(5819..6799)
                     /gene="pta"
                     /locus_tag="ACHAUN_000477"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8459207.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6952..7632)
                     /locus_tag="ACHAUN_000478"
     CDS             complement(6952..7632)
                     /locus_tag="ACHAUN_000478"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8459208.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7758..8627
                     /locus_tag="ACHAUN_000479"
     CDS             7758..8627
                     /locus_tag="ACHAUN_000479"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8459209.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8653..9168
                     /locus_tag="ACHAUN_000480"
     CDS             8653..9168
                     /locus_tag="ACHAUN_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8459210.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9215..9778)
                     /locus_tag="ACHAUN_000481"
     CDS             complement(9215..9778)
                     /locus_tag="ACHAUN_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8459211.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9833..10012)
                     /locus_tag="ACHAUN_000482"
     CDS             complement(9833..10012)
                     /locus_tag="ACHAUN_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459212.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10089..10598)
                     /locus_tag="ACHAUN_000483"
     CDS             complement(10089..10598)
                     /locus_tag="ACHAUN_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459213.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10694..11074)
                     /locus_tag="ACHAUN_000484"
     CDS             complement(10694..11074)
                     /locus_tag="ACHAUN_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8459214.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11404..12153
                     /gene="pnuC"
                     /locus_tag="ACHAUN_000485"
     CDS             11404..12153
                     /gene="pnuC"
                     /locus_tag="ACHAUN_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8459215.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12474..13691)
                     /locus_tag="ACHAUN_000486"
     CDS             complement(12474..13691)
                     /locus_tag="ACHAUN_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8459216.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(13949..15226)
                     /locus_tag="ACHAUN_000487"
     CDS             complement(13949..15226)
                     /locus_tag="ACHAUN_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459217.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15305..15949)
                     /gene="pcp"
                     /locus_tag="ACHAUN_000488"
     CDS             complement(15305..15949)
                     /gene="pcp"
                     /locus_tag="ACHAUN_000488"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8459218.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16148..17092)
                     /locus_tag="ACHAUN_000489"
     CDS             complement(16148..17092)
                     /locus_tag="ACHAUN_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8459219.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17094..17780)
                     /locus_tag="ACHAUN_000490"
     CDS             complement(17094..17780)
                     /locus_tag="ACHAUN_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8459220.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18254..18574
                     /locus_tag="ACHAUN_000491"
     CDS             18254..18574
                     /locus_tag="ACHAUN_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8459221.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18728..19633
                     /locus_tag="ACHAUN_000492"
     CDS             18728..19633
                     /locus_tag="ACHAUN_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459222.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19630..20403
                     /locus_tag="ACHAUN_000493"
     CDS             19630..20403
                     /locus_tag="ACHAUN_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8459223.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20584..22008)
                     /locus_tag="ACHAUN_000494"
     CDS             complement(20584..22008)
                     /locus_tag="ACHAUN_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8459224.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22219..22665
                     /gene="nrdI"
                     /locus_tag="ACHAUN_000495"
     CDS             22219..22665
                     /gene="nrdI"
                     /locus_tag="ACHAUN_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8459225.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22730..24712)
                     /locus_tag="ACHAUN_000496"
     CDS             complement(22730..24712)
                     /locus_tag="ACHAUN_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8459226.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24726..25622)
                     /gene="murQ"
                     /locus_tag="ACHAUN_000497"
     CDS             complement(24726..25622)
                     /gene="murQ"
                     /locus_tag="ACHAUN_000497"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8459227.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25625..26686)
                     /locus_tag="ACHAUN_000498"
     CDS             complement(25625..26686)
                     /locus_tag="ACHAUN_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8459228.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(26897..27424)
                     /locus_tag="ACHAUN_000499"
     CDS             complement(26897..27424)
                     /locus_tag="ACHAUN_000499"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8459229.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(27491..28993)
                     /gene="eat(A)"
                     /locus_tag="ACHAUN_000500"
     CDS             complement(27491..28993)
                     /gene="eat(A)"
                     /locus_tag="ACHAUN_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8459230.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29251..30372)
                     /locus_tag="ACHAUN_000501"
     CDS             complement(29251..30372)
                     /locus_tag="ACHAUN_000501"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8459231.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30498..31439
                     /locus_tag="ACHAUN_000502"
     CDS             30498..31439
                     /locus_tag="ACHAUN_000502"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8459232.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31589..32485)
                     /locus_tag="ACHAUN_000503"
     CDS             complement(31589..32485)
                     /locus_tag="ACHAUN_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8459233.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            32754..34094
                     /locus_tag="ACHAUN_000504"
     CDS             32754..34094
                     /locus_tag="ACHAUN_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8459234.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            34312..34755
                     /locus_tag="ACHAUN_000505"
     CDS             34312..34755
                     /locus_tag="ACHAUN_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8459235.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(35259..36677)
                     /gene="nhaC"
                     /locus_tag="ACHAUN_000506"
     CDS             complement(35259..36677)
                     /gene="nhaC"
                     /locus_tag="ACHAUN_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8459236.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(37080..38303)
                     /locus_tag="ACHAUN_000507"
     CDS             complement(37080..38303)
                     /locus_tag="ACHAUN_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8459237.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(38334..39173)
                     /locus_tag="ACHAUN_000508"
     CDS             complement(38334..39173)
                     /locus_tag="ACHAUN_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8459238.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(39190..39870)
                     /locus_tag="ACHAUN_000509"
     CDS             complement(39190..39870)
                     /locus_tag="ACHAUN_000509"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8459239.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(39872..40945)
                     /locus_tag="ACHAUN_000510"
     CDS             complement(39872..40945)
                     /locus_tag="ACHAUN_000510"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8459240.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(41001..41239)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(41282..41515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(41592..42263)
                     /locus_tag="ACHAUN_000511"
     CDS             complement(41592..42263)
                     /locus_tag="ACHAUN_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8459241.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(42350..44998)
                     /gene="mgtA"
                     /locus_tag="ACHAUN_000512"
     CDS             complement(42350..44998)
                     /gene="mgtA"
                     /locus_tag="ACHAUN_000512"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8459242.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(45340..45514)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            45687..46124
                     /gene="ndk"
                     /locus_tag="ACHAUN_000513"
     CDS             45687..46124
                     /gene="ndk"
                     /locus_tag="ACHAUN_000513"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8459243.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(46146..47012)
                     /locus_tag="ACHAUN_000514"
     CDS             complement(46146..47012)
                     /locus_tag="ACHAUN_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8459244.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(47172..49265)
                     /locus_tag="ACHAUN_000515"
     CDS             complement(47172..49265)
                     /locus_tag="ACHAUN_000515"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8459245.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(50014..50637)
                     /locus_tag="ACHAUN_000516"
     CDS             complement(50014..50637)
                     /locus_tag="ACHAUN_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459246.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(50796..52193)
                     /locus_tag="ACHAUN_000517"
     CDS             complement(50796..52193)
                     /locus_tag="ACHAUN_000517"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8459247.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(52225..53196)
                     /locus_tag="ACHAUN_000518"
     CDS             complement(52225..53196)
                     /locus_tag="ACHAUN_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8459248.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            53570..53995
                     /locus_tag="ACHAUN_000519"
     CDS             53570..53995
                     /locus_tag="ACHAUN_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459249.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        15719 a        11284 c         9619 g        17831 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     3061 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3121 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     3181 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     3241 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     3301 cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca
     3361 aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca
     3421 ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg
     3481 aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca
     3541 tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat
     3601 aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat
     3661 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     3721 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga
     3781 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     3841 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     3901 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     3961 ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg
     4021 tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt
     4081 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     4141 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     4201 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     4261 tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt
     4321 gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct
     4381 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     4441 ttggacttat taccgcagat gcaagctttt ggacaccgtt taccaagagg ttgtgaaatc
     4501 agcgttttga cctgagtatt agagaaacaa acggcaaaat agttcctcat attttgccgt
     4561 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttattcaaaa aaaatggact
     4621 gcgacacgac ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa aaggatgaca
     4681 gtttctcact cttttcctat ttcttctatt caatcaaata actcatttta tatacatcta
     4741 aaaattcacc tagatctgtt cttgcccctc taggcatgac cgcttcgatt tggaagccca
     4801 tttttcggta aaggtgtacg gctctttcat tacgaacttg aacgtcaagc tccaatcgaa
     4861 aaagtacacc catttccttc gcccaactga tgatttcttc tagcataaga gtgcctagtc
     4921 ccattcccca atattcctgc agaatactga taccgacttc tcccacgtga gacaaacgaa
     4981 attgactgtc tgccttgaca gatgctgttc caataatcgt cccttcatag attgcaagca
     5041 gcaataaatt attattgctt tcgtatatat aatccaactc ttcttctaag gtttctggtg
     5101 gtaacaggag ttctgcttca tccaaaacta aaaattctgt ctccttatta acttgtccca
     5161 tcatcaaaag aatgtctttt gcatctcctg gcactgcttc ccgcaagatg atcccaactt
     5221 cttcactcat gattttcagc ccatttttct agaattcgct gtttgaaggt ttcactttgc
     5281 tccccgtatg agattagttt cacataccgc ttttctaaat ctgtatcact tttttctaaa
     5341 atcagttcca aataatcctc cggcagtgct tcttctaata gatttcccca ttcaacaacg
     5401 gaaagcccat caccttcaaa atattcatct aaccctaaat ctgctccgct agctgctacc
     5461 cgataaatat ccatatgata caacggaatc cgaccttgac tatattctct aataatcgtg
     5521 tatgtcggac ttttgatcat ttgcgttatc cctaatcctt gggcaattcc tttcgtcaac
     5581 gttgttttac ccgcacctag atcaccagtc aatatcagat tgtcacctgg cattgcggaa
     5641 tgaccaattg cctctgcgaa ttgttctgtc attgttaatc ctgatagttc gatcattttt
     5701 catcttctcc cgctcttctt tctttttgcc agtatatcgc gtaatgaagt ttttttaaag
     5761 aaaaaatgaa ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg tgtatttgtt
     5821 aatcaatcaa tgtttgtgct gctgtgataa tcgataattt atatacatct tcttcatttg
     5881 ctccacgaga caaatcagaa accggtttgt tcaagccttg tagaattggt ccgattgctt
     5941 caaagttacc gaaacgctga gcaattttat agccgatatt tcctgactgc aattctggaa
     6001 atacaaagac tgttgcttga ccagctactt ctgaatctgg cgctttcagt tgtgcaactg
     6061 aaggaacata agaagcatca aattgcaatt cgccgtcgat catgtattgt ggcgctagtt
     6121 ttttagcgat tttagttgct tcaactactt tgtctacttc tggcgatttc gctgagcctt
     6181 ttgtcgagaa gctcatcatt gctacttttg gttcgatatc gaacaattct gctgtttttg
     6241 cagaatctac ggcgatttca gccaattctt gcgcagttgg attcacgttg atcgcacaat
     6301 ccgaaaagac atatttttct tgatctcttc cacgtaccat gatcatcgct ccgcttgtcc
     6361 gactgatgcc aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg tcgccagtcg
     6421 aatggatcgc tccagagacc attccgtctg caatccccat atacgtaagc atcgtaccaa
     6481 agtaattcac atcttttaaa atcttttctg cttgttcttt ggatgccttt ccatttctac
     6541 gttcaacaaa agtatccacc atttcatccc acttatcgta attatcaggg ctgatgatcg
     6601 taaagctaga tgttttgatt cctctggcat gcgctgcatt tcttatttct tcttcgttcc
     6661 ctactaaaat gggttccaca agttcttctg ctttcaaccg ggcagctgcg cctaaaatac
     6721 gagcatcagt agcttccggg aaaacgattt tgatcccacg gcgaacaatt ttaaatctta
     6781 aactatcaaa caattccaca agatgacctc cagacgtttt tatatgtatt atcatacact
     6841 attccagaaa aaaagaacag tttattttat ctgtatcagt caaaaatgct ggaaaaatta
     6901 agtttttcac atacaaagcg aactgcacta taacaggaaa gcgctgtttt attacactgt
     6961 ttcaggcagt tgccaatcaa tcggttcttc tcctaacgca aggagcgcat cattcgtttt
     7021 cgagaagggt ttggaaccaa aaaagccacg acttgcagat aacggactgg gatgtggaga
     7081 tgttatgatg atatggcgac tcttatcgat cattttgatt ttttcttgtg ccggttttcc
     7141 ccacaagata aacacgattg gtttttcccg ttcatttaat ttttcaatga tcgtgtctgt
     7201 taatctttcc caacctttcc cgcgatgaga atacgcttgc ccttcccgaa cggtaagaac
     7261 ggtgttgagt aaaaggactc cctgtttagc ccatgacact agatttccat gttgaacagg
     7321 tgcgattcca agatcatcat aaagctcttt atagatattt cttaacgatg gcgggatctt
     7381 tacgccaggt tgcacagaaa aagataagcc atgagcttga ttttctccat gataaggatc
     7441 ttgtcctaaa atcaccactt taacttcttc ataaggagtc aattccaatg cttcatagat
     7501 atgatacata tctggatgga tcttttggga cgcatattct ttttttaaaa attctcttag
     7561 atgcagataa taatcttttg aaaattctga ggacaaaact tcttgccaac tgttatgaat
     7621 gatttctttc aaaaactcca cctcctaata aaaagaataa ctcacatttt cttcaatcac
     7681 aagggacaat cttcaccctc agcacttgat taatatggta aactaggaac aagaagcaga
     7741 aaggaacgat aatcattatg atcaaattaa ttgcatcaga tatggacggc acattattag
     7801 atgcccatat gagtgtttct cctgaaaatg ccgaggcgat acgttttgca aatgatgccg
     7861 gtgttgagtt catggtcgct actggtcgaa actatcaaga agctcgcgca gctttagatg
     7921 aggtaggaat cgattgtgcg atgatcactt tgaacggggc acaggtattt gataaagaag
     7981 gaaattcatt gtttaccgtg tcgattccaa ataatcaagc aatctctgtt ttagatattc
     8041 tagatgctaa cggtatttac tatgaagtag caaccaacga tggtctttat tctgaaaatc
     8101 aagcgaaacg aattgaaagt tttgcttcca tggtggctac ccatttacct caccttacgt
     8161 ataaaatggc gattgccatg gcctcagcta agctggagtt attgcacatc acatacgtgg
     8221 acagtattcg tgctattttg gaagaaaaga atctggaagt tttaaaaatt atttgttttc
     8281 atacagaagg gccagctgta ttaggtccag ttggaaagca gatttctctt ttaggcgatc
     8341 tagccgttac ttcctctggt caaaacaata tcgaagtgaa ccataaaaac gctcaaaaag
     8401 gaattgctgt tgcccatgtc gctcacgagc gtgggatttc tttagaagaa gtcatgacga
     8461 ttggcgataa cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt ttcgcaatgg
     8521 gaaatgcaga aatcgaagta aaagattatg ccaaatatac aacggacaca aaccttgaat
     8581 ctggcgtagg aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt tatctagaaa
     8641 agagggatat agatgtcaga atttttcatt caggaaaagc agcttggacg tgcaactcgg
     8701 acaattgtca aaaacgaaaa aggacattcc ctgtttttga tggttggtcg ctggggaaca
     8761 aagggcgatg cattgtctct ttatgccatg aatggcgatc tcgttgctca catcaagcag
     8821 ataagcctga cattcggtac gcgttttgag ctgtataaag agttcgaaaa ggtgggaacc
     8881 atgcagaaaa tattcaattg gcccggtgac ttttattata ttcgtagact tcactggaca
     8941 gtccaaggtg atatctataa tcatcaatat caaatccatc atttcaatca gcaaatcatg
     9001 aaaatggata aagcaacact tttttcagga gattactatg tattggatgt ctcaaatgat
     9061 gaagatgctc cagtttgtat ttgtattgct gcggttttag attactggct ctaccgtaaa
     9121 gacaaaagtc gcgataagcc tgtttcttac cgacttaact taagttaaaa agatgtacct
     9181 gaaatgcgga aatacgcatt tcaggtacat ctttttagat attcttttgc atatgatcat
     9241 aaagatgacc actgatcatc cgctgaccga caactttgaa gtcatggcgt tcgtataatt
     9301 ttttagcctt tggatttttt tcatccacac ttagaccgat gacggagcga ttagctttct
     9361 tcgccaattt aggtaatgct tctaataatc gcgaaccgat gccttgtcct ctaaaatctt
     9421 ctgatacact gatagagtcc aaataccatt cgttgggaaa cgtttcttta tcaatgaaca
     9481 aacgtgtaga cgcatctagc tgatatttct ctaagatagg taccagtggc tgatcgatga
     9541 ttgattcttc ttcatctgta taaccgtaag caactcctgc aactttaccg tctatttctt
     9601 ctacgatacc tcgtgaataa ctgtaacgat aatttggatc agtgattgct tcttctaaaa
     9661 tctctaaagt cttttgttcc cctgtctgct tcaaaaatgg cagttccata tcttttagaa
     9721 tcactaagat caatggggcg atttctggag catcttcttt tctcgcaaaa cgaatcatag
     9781 tttcctctcc ttttctccat tctaacatgc agaaaaagaa atcgcaaaat cgttatttta
     9841 aaaaatcttc aatcttttca gtaatgaaat aaccatgagt aacgattctt gccgcttcag
     9901 ttcggcaata agggcagacg actggcattt ctttatcatt atgttcgact acattattga
     9961 cttcttcgta tgcaaatttt tttccgcaat tagggcattt cattttttcc atgttatctc
    10021 aacttccttt catctttact ctcttagctt actcttatat tgtaaaaaag agaagcaata
    10081 tccttcttct atgcagatat ccatacctga cggtcctgtt ctccatctat taatcggact
    10141 ttataataag gacgttcatc tttgactaga agtgtccact ctttcacttt tgcttttttc
    10201 ccgtagtagc tgactgcttc cttcatcgct gcttgcggtt gtttgaactc ttccgttttt
    10261 tctatatcaa aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt tgttttccca
    10321 gtggatggat tgactgctat gacttgtcct tcacaaaaaa actggtattc atatgttgca
    10381 ttcgtaggat tctccaaatc tttttcaact gcaaaatgaa tagcagatat gttatgttct
    10441 tttgtttcgt tttttacgat tgctgctgct tttttcaatg agatccgata ttcctggtca
    10501 ctaatatcat ccatgtgaaa agcggcattg agcagtcccg tcataccggt aatccctatt
    10561 acgatgaggg cagcaacacc aaacatcatt tttttcattc ttcattctcc tgtttctttt
    10621 ttttaaagaa tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa aaaaagtgac
    10681 aattttgtta tctttattcg tgcagttcta atggcaatcc gtctggatca aagaaaaacg
    10741 tcattttttt tcctgtaaag gtatcttctc taatcggttc tgattcgatc cctaaggact
    10801 gcaattctgc aattgtttct tcaatatcgt ctactttgaa agcaagatga cgcaagccac
    10861 aagcttctgg atagtttggt cgttgtggcg cattagcaat tccaaaaatc tccaattcag
    10921 cacttcctag tttcagatct aatttatagt catctcgttc tttgcggtaa ttttctctga
    10981 ttacttcaaa tcctaattta ttaacataaa aatcacgtga tttctgataa tctgaaacaa
    11041 tgatggctat atgatggatc gttgtaaggt tcatatgagc actcctattc ttcgtttaag
    11101 ctcggatcta tattaatctc cagttccatt gtaaatgtgt cagattgaaa aaaatagctg
    11161 tttttccttt ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc caagacactc
    11221 ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa aacaaacaga
    11281 aaattatcat aacacaaatt atttataatt gaaagactag ttttttttca agaaaagcat
    11341 acaatagatt gtgttagtaa atacagaaca cactacattt agtgtcttaa ggagtcctca
    11401 tacatgaagt catattttca gtttttagct catcagctaa aaggctggcc gcaacaaaat
    11461 tattatcttt tctttttcag tcttggctgc caggtcatga ctcttgtcaa tagtcccatc
    11521 acttggctgt ccgttattac atttatcggt accacgcttg gtgtattgtg tattttatcc
    11581 atcaatgcgg caaaatcagt caacggtatt ttaggtattt tgtctgcgat ttgttttatt
    11641 atcgttgggt tttcagcaaa gaactattta agtatcggtg aacagattgc ttatatgatc
    11701 acattggata tccccgtatt gttaagtaaa gaatggaatc ataatatggc ttcaaaaatc
    11761 cgaaaattca ctggaaaaag ctggtttatc gcaatcatca gtactttcct tgtctatctt
    11821 gtttcaggat tcctgattgg tcgttataca gatgatcctc ggccgtgggt agatgccatt
    11881 gctttttcta ttagcttgac tgctggtatc atcagtttct tgcgttacaa caaccaatat
    11941 tactggtggt tagcttcagg gcttgctcaa atggtccttt ggtatatttc atttacgcat
    12001 ggatctgcaa gtttggcgat gttcgtgaac agctctgttt acttgctgaa tgatgtcctt
    12061 gcatttaccg tttcaccttg gtataaccaa aaagaacgtg aacgtatgca aaaaatggaa
    12121 gaagaatact acaacgcaca acaacacgca taaaaaaact gagcgtggga caaaagtaaa
    12181 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaatcagaag caactccttc
    12241 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
    12301 cttggagtta aacgcttctg tcccagcctc tatttcctac gattagtcaa gtaaaaggtg
    12361 caagaaaaat agctcgtaaa cgaaagcgac aaaatcaaag cacaccaaat aaagccccgt
    12421 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
    12481 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
    12541 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
    12601 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
    12661 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
    12721 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
    12781 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
    12841 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
    12901 tcggcttttt tgatcatttg tgtcgccagt gctttttttt gttcgagtaa atcttggaga
    12961 agttcggcgt aatagatcgt tttttgacag tggatactgt ctttttcaac agctggatag
    13021 gcacactgtg catagtggat gatttgatct gccttttgct cggcacgatt cgcggagatt
    13081 tttttccgtg tttcgttgat cagtttattt tttatttttg tccgagtaga tgccaataca
    13141 aaatctggat ggggaaaaag acggatcaat gtcaatgcgt aaggggtcag cctgtttgaa
    13201 aagaactgtt caagctctgg aaaagtcaat tgaaggcagt tgtgtaaatc catgcgcaga
    13261 cgagtgattt tcttctcaat ttcttgataa aaacgggcta aatcgcgcat ctctaagtaa
    13321 aaattgcttt gtacttcttt tggctgacgt tgatttttaa gatgagtctg tgcgagccgg
    13381 tgggcatccg atttatctgt tttccaactg cgtaaggtgc tttcttccat ctgttttttc
    13441 gcctctaacg ggtttaacaa acagtaagaa aagtgatgat cttgaaagaa cttttcgact
    13501 ggtctggaat aaatccccgt tgcttcaaaa acaatcaatg gacgctcagg caagtgctgg
    13561 atctcttcga gtaatgcatg aaatcctctt tgagtgtgtt caatctcgtc ttcaaataag
    13621 caggtgtcgt ctttgtatag gacactgtag ctgtgtccca ttgaaacatc taatgcaaga
    13681 atgtaaggca tttgttttcc tcctttttaa gttttgaaag aactttactg cttcatttcg
    13741 attccaattt cctatacacg ggctcatggc cccaacatac gtaaacctga ttctaaaatg
    13801 acagtaaaga agttcggttt ttgataggga ctcgtagtcc tcaaggcttt gttcgaactt
    13861 tctttcactc ttcactataa tctaaaaata gaaaatagag gagaacatca ttcgtcaatg
    13921 atgttcccct ctaatcttag tatgtttttt atagtaattc cttcagatac tttcttgcct
    13981 catcattgaa acgcagtgat tcttctaact tctgataata ttccaacgct tgtttctgag
    14041 cttcagtaaa acgataatgt tttcctttcc atttgtcgat tgtctcttgc acatttccag
    14101 attggatatc tttcactact agttgccatt tttcttttga gactgtacgg tctttcttat
    14161 cagcagattg tatagcttct cgttctttac gcaatacttt taaaaaacca tttctgacta
    14221 tccgaggaat gctgtcgaag aacccttctg taaaaccata gcgacaatat tgcttccaaa
    14281 taaatagata gatttttggt tggatgaatc gttggttttt cgcaaaatat tccatctgag
    14341 cagtgacttc tggtgttcgt tgacgttttg tctttctcaa cacggaaata aatgtttcaa
    14401 aataagcatt aggatgtact ggcacttctt catatacgat ggattgccaa tctttacgta
    14461 aaaatgcgcg atagttgatc aagaattctt ctacatcctt tactaattct tctgcaaagt
    14521 cgtcaaatag ttcttttttt atctgtcgct tttttgactg cccattgatg tgttcactta
    14581 ttttcttgat ttcattttcc aatgcttgat aatccgctat gtaaatcggc atttctactt
    14641 ggcgaaattc tgcgagccgc tgagctaatt ttatatccgg taaatatggc tgtttcatcc
    14701 gacgattttg gtacaagaca aaacagtatt tttgccatag tccttcagga aaatcgatat
    14761 tcttctgctt aagccataat aattcgtcta aggccagatc aacttttgtc attccctgat
    14821 ggatcgaagc tgccactgtc ggagagacat gcaggtgatc ggcaaagtgg ttgatgccta
    14881 gttttaggat ctttccggtt tttggcgatt taacgatgta ttgcactttt aattctctac
    14941 cacactcaca atacaatttt tgctggcttc gttttgttcg aaaaaacgga ttgatcttga
    15001 agtcagaaaa cacccaatct gttgcagcta aataatgttc ttccataaaa cgagagctga
    15061 ttagatatcg gcggtgctta tcgactagtt cccgctgttt tttcgttaat tttgcataga
    15121 tctcccaacg ttgactgctt gtgtattgtt tagaaatttt gatgggatag cttttgctga
    15181 tttcttttgg aaaccattct tcaaagacgg attgccattc ttccacggct gtttctttcc
    15241 tttctaatta caaaaagctg gctactaaca taacgttagc aaccagccag tgtttcttgc
    15301 taaattagtg agttgttccg gcatttccgg aaatatcttc tttattttct actgctgctt
    15361 caactgcata agtcaaagaa gaagcaatgt cttctaaaga catagaaggc gtacccactg
    15421 gacgatcgat cacttgatcg ggtgagaatg gcacgtggat gaacccacct cgaattgccg
    15481 ggaattgtgt ttcgatcaaa tgaagcaaac gatacatgat atcattgcag acaaatgttc
    15541 ctgctgtata agagatgtac gctggtagac catgatcttg tacatttttt gtcattgctt
    15601 tgattggcaa tgaggtaaag taagcggctg gaccgttttc ttctaaagcc gtatcgaatg
    15661 gctgatttcc ttcattatca gggattcttg cttctgctag attgattgcg acacgttcga
    15721 acgagactgc tgaccgccca cctgcttgtc cgacacagat cacgacatct ggttggtgct
    15781 gttggatggc ttctttgact acttgactgc ttttttcgaa tacggtcggt acttccactt
    15841 tgatgatttc agcaccgctg atttcatctg gcattttttt tactgcttca taggctggat
    15901 tcactttgtc cccaccaaat ggatcaaaac ctgttactag tactttcatc tgttgttcct
    15961 ccgtccgatt gatttaacat atggttatct tttatcatac cattttcctt gaacagcgta
    16021 aataaaggaa acaatagttt aaataacata acagatcaaa cttaatatat tcttataaaa
    16081 aaatagtgtt ccactacaat gttagaagag gttgcgacaa ttattctgtc acaacccctc
    16141 tcttctatta tttaaataga atcatataag caatttggaa aactagcata aataacgcaa
    16201 taaacaactg gtttttgatc actccgtatt tgtctttcat ctccagcatc gctacaggca
    16261 cgatattgaa gttagcagcc atcggtgtca gcagggtccc acaaaaaccg caagtcaatg
    16321 caaccattcc tattaacgct ggattcgctc catgactaaa tacgaatggt gcaccgatcc
    16381 caaccgtcat cacggtgatg gccgcaaatg cattccccat gattaccgta aatatcacca
    16441 tgccaagagc gtaaatgatg atgccgatgt tcacatttcc ttctggaacg atcgtaccta
    16501 ctgcattgga aatcactgtc ccaactccag cgcttgtaaa taccgcacct agagaagcta
    16561 aaagcattgg caacatgctt aacggcccta ctgtgcccag catatctgct gcatcatcta
    16621 aaaatgtact tggtcgatta tctcgagaaa agaacatcaa gattagcatg gctgcaaaaa
    16681 caccgacgcc cacgccgact aaagcaccta aattggtaaa tagagcaaac aagatcgcaa
    16741 agacaccgat actcaaagct ggaataaaaa ttttcattcc tagtttgtct gacatttttt
    16801 ccatatattc ttttgatgga agccggcttt ctcctttgct gactttctta gcaattgctg
    16861 gtatggtcat cgcaaagatc aagccaccgt tcacgattgc cggaagccat cttcctcctg
    16921 caataactac ccctaagacc gtccagaaaa aggctgaccc ataacgatga ggattggttt
    16981 tatctgttaa atttcttact ccagtgtaaa tcaatacgag tcccatcacg atataaagga
    17041 tctccagtaa tttatcgcct aataaaatat caggatttgt aaaaaagtcc atcttattgc
    17101 ccctttcctg gataatattt cttcgctaat ttcttttctt taagatagaa gtagacactt
    17161 gccacaacta aagccgtgat agccactgga acttccacta aagcaagctg tcccaatgtt
    17221 acgtcatatc ctagatcttt cagtgttgat tggactagca acgcccctgc tcctccgatg
    17281 aatagtactt ggccaaagaa ccagcagaca ttttccatag ctgaagccat accttttagt
    17341 tcttcttcat gcttaggatg gacttctaac ccttttgatt cgatggtccc caatgccatc
    17401 ggtaaaatga ttggacgaac aaatccagca actccaccaa aactcacgtt aaaagccgca
    17461 aagatcattc gaaagatccc ataaatatct acaatcatac cagcagatac tttcttgaat
    17521 cgcttgatca aggaagccgc tgattctttc agaccatttc tttcaagcgt tcctgttgcc
    17581 aacataatga tgataaaaat cgccatcccg cgattgttta caaaacttgt acctaaaagt
    17641 tctaacattc cttgaatgcc aagaccgcca gtcaaagctg taacaactaa agcaaccatt
    17701 acgatcaata tcgagtctaa tttaaatgca aacccgagaa taacaatcaa gacgcctaaa
    17761 aggctaagat aattttccat atattttcct ccataaaatg acaatatttt tttaattata
    17821 aagcaaaaca cataaaaaat atagttgttt tctcacgaaa tagacactat ttttaaatat
    17881 ttttcgttat ttttttatca caaaaattat cttacttatt gtaaggtaaa gcttttttta
    17941 atttattgcc cgataatata tagaaataag gtctagttac tgtttttttt aaatgattaa
    18001 taaataacaa cttctttcta tgaaattgca aagcattcat cttcttttcc gctaacgata
    18061 tatccgtcag tgcttggtac actttactaa aaagtatgag attgccgata tctggatagg
    18121 tgtgctatac tacagaaaaa gatatacaat ctgccaccgg gtaggataaa gaggatgatc
    18181 attccattag gtctttgaag ggatgatttt cctctttttg tgtatttttt tatagttaag
    18241 gaggaatgta agtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg
    18301 gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt
    18361 gattggcatg attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgccgatgag
    18421 tctctcttat cctatctgga cagggattgg cgcagttggt tcggtcataa ttggtgtggt
    18481 cttgtttcat gataagctga atctttccac ttggttcttt gtggggatgc tactcatcag
    18541 tatcattggc atcaagatta ctagcggcca ctagacactt ggaataagta ctgatcagaa
    18601 aaaataagcc tttttattta atattcaatt tttttatgaa aaaataagaa ataagtccaa
    18661 tgacctagcg acttctccac tcatcaggtg tatgcttgta ctatctatag aaaaggagtg
    18721 aatatttttg aaaagtcttt cggtccaaga attatccaag aactttggtc acaaacaagc
    18781 cctcagcgat gtcagtttta gcttatcccc tggcaaaatc gttggattag taggtcctaa
    18841 tggcgctgga aaaacaacga tcatgaaagc tattttagga ctgatccatt actcagatgg
    18901 aacaatctgt attggtgaaa atgagatttc tccctctagt cataaagggt tggaacaagt
    18961 aggagcattg atcgaatacc cgggtatcta tccattctta acaggctatg atcatctgaa
    19021 gttattctct gaaaccaacg atgtatctgc tatcgacact atcgtgaatc agctcaagat
    19081 ggaaaagtac attcacaaaa aagcgaaatc ctactcgttg ggaatgaaac aaaaattggg
    19141 aattgctctt gccttactga accatccgtc tatcgttatt ttagatgagc cgatgaacgg
    19201 tttggatcca caagcaacaa gagatgtgcg aaaaatcatt accgacttgg ctactcaggg
    19261 cacctctttc ttgatctcca gtcacttact cagcgagctt gaaaaaatgg tcgatgagct
    19321 gatcctgatc aatcaaggaa agatcatcaa acaatgtaca atggctgaat tgaaccaaac
    19381 agcgcaagat ttcatcgtta tctctacaac aaatgatcta gctgctcgaa cgatcttgac
    19441 agaagctgga tatactttgg aagatcctga taaaatcaag cttttaaaag aaaacgaaga
    19501 attattagga accgtgatcc agctattgtc tgcacaaaat atccatgtga cagacgtcca
    19561 gcatctgacg aatgatttgg aatcttctct attggaaatt ctggattcag taaaggggga
    19621 agaagaaaaa tgacattgct tgtaaaacaa gaattattta aattgatcaa gaaaaagtca
    19681 actgctgttt tatctgttct attagttgtt ttgctcatag gaacagcact tctagccaaa
    19741 aaatacacga cgatcattga tccagttgaa atgactgctc agctttttag tgccacatct
    19801 tggattgtct ttatcatgat tgcagctgca agcacgatca tttcgatgga agcacaatat
    19861 ggtacattga aaaatctcct ttatcgaaag tattctcgag gagaaatatt agtaagcaaa
    19921 tggatcacat tagttattta ttctgtttat ttataccttt tagctattat tgtcactgtg
    19981 ttgatgaaac tgattctttt cccttcgatt tccttcacgg agaaggtttc tactggtcaa
    20041 acactgatcc aaagcttgtt cacttatact ttgggtagtt atatcggttt atggctgatt
    20101 ttaagtttag tgttgatgat tgcttgcttt atcaatagtt ccggcgcatc catctcagca
    20161 ggaatcgtct tttattttgc ttcgtcaatc atatctggta tccttattgc actcatccaa
    20221 aaatgggagt ggatcaaatg gaatccgatc agtatgttga atctgcaaaa ccaaattgga
    20281 aacgaagaga tcatgaagca attgactcat ctttctacta atcaaatgct attcggtaat
    20341 ttagcttaca tcgttttgtt cttagcatta ggatatctcg tatttaagcg aaaaaatgta
    20401 tgatgactca tcaaaaaaga ggctgtgaaa gagacgttca gccttattgc cgattggctg
    20461 tctcttaaac accgtttatt ctgaaaaagg agctgtgaca acatttgtgt cacagctcct
    20521 ttcctagtat gtttttgaat aaacgacagg ctcacaacct ctctttatac ttcttttata
    20581 cttctatacg tgaagcatcc cagtggcaaa taaaaccatt gcaatcactc cgaccatcgt
    20641 cacacctata agtaaaatct tatctaagtg gatatgttgt acaggctcgt tttgctcctt
    20701 acgtgctttg aggtaaaaat aaatgcctgg caagaaaaga acaagaacaa gcattacttc
    20761 attaaaggct acgactagca tagcccatac ttgaaaggca cttgctaaaa gccctactaa
    20821 aaattggacc cattctttcc gctgataaga aagtttcatt tggtacaatc ctaagaaaag
    20881 ccacgtgaag aaaatagctg ctgtaccaag tgtgtaagca aagttataag cactgtcagt
    20941 gaataataat gaaagtaaaa agacattcgt acataacgtc gtcaatacga tagcataagt
    21001 tggtgcccca tttttgttga gttttcccca tgctttaggc aaaagtccgt cttcagccat
    21061 gtttcccatt gtttcaatcg gtaagatcgt ccatgagagc caaacaccaa ctaaagaaat
    21121 gatcaaaccg ccgttgatca cgactgctcc ccatttacca accatcattt caaacacata
    21181 gcccatagca ggctgcgcct tgatcgtttg cagctgattc atcgtcattg tcccatatgg
    21241 aagcatggaa accaagacgt aaataataat caagccgaat atccctagaa tcgttgcttt
    21301 cccaacatct gattgttttt ttgctcgtga acctacgaca gaggctcctt caataccaac
    21361 aaatacccaa agcatcacca tgatgcaact caatacttga ctggaaaacg ggatcggctg
    21421 tccattcgca ttcaatataa tgtcgctaga aaagttatca atgaattgtt ggatcgaaaa
    21481 cgtgctgata gctactacaa taaataaaaa taatggcact aatttacaga atgtgacgat
    21541 agcattgacg aaactagcat tttcgactcc ccgattcact agccatgcat atccccataa
    21601 aaagactgta ctcatcacga tcgatgccag attttgccca ccttcaaata ctgggaagaa
    21661 atatcctaat gcgctcatca acaatgttgc aaaagctacg ttgcctaacc agcatgtgag
    21721 ccagtaaccc caaccgctga taaaaccagc gaaacggcca aaaccttctt ctgcgtaact
    21781 aaagatcccg ccttttaaat ctggacgttt tttattgata ttattgatcg acaaggagag
    21841 catcaacatt gctgttccta cgagtaccca actgaaaata gctgggccgc cagccgatcc
    21901 ggagatgtcc gtcataatac caaatacgcc acctccaatc gctgaaccaa ttactaaggc
    21961 agcaagtgga attaaaccaa ttcctttttt ctgttgctct gtatccatgt aagcatcctc
    22021 caaaataaaa ccgcttttat caaaaagtat aacaaacctt ttttattttt acttaattaa
    22081 taaaaacttt aatagaaata taattctacc ggcttttttc tcattataca agcaaagcgc
    22141 ctagtccctt tccaaattac accaccaaaa aacttaaaaa agatgctata ctaaaaggaa
    22201 agaattggag gatgatccat gaacatttta tatatatcga tttcaggaaa tacgcgtgcc
    22261 tttgccaaac atctagcaga atatgctgaa aaaatgcatg cagaagatcc tgtcaatcct
    22321 gaagttactt taaaagaaat ccatgaaaat agtgatttta cgaaagaaac agaaccattt
    22381 tttactttcg ttcctactta tttagacggc ggaaatggtt tagataacgg tgatacagaa
    22441 atcttgaccg aaacgatgcg tgaatatctg gaacatgaag acaatcataa actttgtctt
    22501 ggtgtagtcg gtagcggaaa taaaaacttc aataatcagt attgtctaac agccaaacaa
    22561 tatgcacaaa catttggctt tcctttcttg gcagattatg aattacgggg aacaccatct
    22621 gatgtgacac gaatttatca gattttagca gaaaacaata aataataaaa aagaagaagc
    22681 tgaaaaatag atttcagttt cttctttcgt ttctattgtt tctatcatat taagctttga
    22741 catccatcac aggtgctttt gcttttgctt tacctgtttt ttccaagaca aagtttttta
    22801 cacgatccat attggttacc aagacgatca tggatgtttc tttgcctgct gctttgatcg
    22861 cttctaagtc agcttctgct acaagcgtat caggtgtcac ttgctgccct tctgttactt
    22921 tcacatcaaa tggcgtaccg cctaagtcaa ctgtgttgat tcccatatga agcaagattt
    22981 ctaaaccatt tggcgttgtg atcccaatag cgtgtttcgt tgggaagaca gagctgacta
    23041 ccccagtaac tggagaatag atatgtccgt tttctggttc tacaaagtag ccttcaccca
    23101 tcatcttttg tgcaaaaact ggatcacttg ctttatctaa agaaacaact gttccgtcag
    23161 caactgatga gaggatagtc acactttcag ctgaagtttg aggtactact ttttcaggca
    23221 cttcatttac tgtggctgtt tcagtcgcta attcttcttt ttctaaacgg tctttaggaa
    23281 taccgaagaa gaatgtagca acgaagccac cagcataagc tgccaataat ccaaggatgt
    23341 agcctaacca tttaccgtca gagatcaatg ggatcaaagc ggctcctgaa ggtccaatag
    23401 cgatagcccc gacatttccg atcattccga tcactgcccc gccgatacct ccaccgatac
    23461 aagcagtaat aaatgggcgt cctagaggta atgttactcc gtaaatcaat ggttcgccga
    23521 ttcctaggat cccgactggt aaagctcctt tgatcatttc tgttaattct ttatcttttt
    23581 tgcagcgaat ccataatgcc aatgctgcac ccacttgtcc tgcaccagcc atcgcaagaa
    23641 ttggaagcaa tagcgtacga ccagtttcat caatcatttg cacatggatt ggagtcaaaa
    23701 tttgatgcag tccgaacata accatcggca agaataatgt gcctagaacg aagccggcaa
    23761 atgctccacc aacgccaagt acccagttga tcccaccgac catgccatca gaaatgaaac
    23821 ctgctaaagg catgatgaag aaaatttcaa tcagaccgat ggcaatcaat gaaagagttg
    23881 gtgtaacgat gatatctacc gcatcaggta ctactttatg cagttttttc tcaacgattg
    23941 acaatagcca aacagcgaag atgacaccta ggatcccacc ttgtccagct gcaagagctt
    24001 caccagtaaa taagtttttc agtggtgctt ccggattcat ccctgtcagc atcacaacgg
    24061 ccccgataac tccaccaaga gttggtgttg caccaaaaac ttgtgcagca ttgatacctg
    24121 tatagataac taggtagcta aacatcccgt ttttcaagat gttcatcaca tcgatatatt
    24181 gttgccatgt tgctgcatca agtgttccgg ctgtgactaa gtttgctaaa actgcggcaa
    24241 tccctgccac gatcccagta ccgacaaatg caggaatcat tggcacaaag atattagaaa
    24301 tgtcttttaa taccgatttg attttagatg gtttttgttt cgatttttgt gccgctttca
    24361 tttcttgtgc ccgttcgttt acacggtctt ttccagatgc tgatgaattt gctgtagctg
    24421 tacttacaga agaaagtttt tctcctaatt tcacgcctgc caaatcaacc atttgctgtg
    24481 ctactttgtt cactttccca ggtccgatga tgacttgaag ctgttcatct tctactacac
    24541 caagcactcc tggaatcgct tttaattcag agacatttac aagatcatca ttgatgatct
    24601 tcatacgcac acgagtcatg cagtggacaa tgctttctac attgcccatg ccgcctgccc
    24661 ctttataaat ttgttttgcc agttcttcca aagtcagttc gttttttgcc atggtctttt
    24721 ccccctcata aggtcccttt tacaaatccg tttgcttcag ctaattttgc ttgagcttcc
    24781 tctttatcac tatttgtcaa aatcatgacg atagccagct taacattttg atctgcttct
    24841 tcaaagtact tttcagctgt ttcgtaatca gcttctgtcg cttgcatgat gattcgtttt
    24901 gagcgttcga ttagtttttc atttgttggt ttgacatcga ccatcaagtt attgaatact
    24961 ttacctatac caatcatact gatagtagaa atcatattca agataagttt ttgtgctgtt
    25021 cctgacttca atcgagtaga gcctgtcaaa aattctggac ctgcatcgac ttcgattggg
    25081 aaatccgcgt gtttactgat ttcggcttgt ttattgcaag aaatcgttcc tgtgacagca
    25141 ccgatcgatt ttgcgtaatc taatccaccg atcacatatg gtgtacgacc gctagctgcg
    25201 ataccaataa ctatatcatt ttttgtcaaa ttcagcttga ttagatcttc ttgccctagc
    25261 tcataagaat cttcggcccc ttcgacagct acagtcattg cttcttctcc accagcaatt
    25321 agacctatga ccatttccgg ttctacaccg aatgtcggta cgcattcagc agcatctaac
    25381 acacctagtc ttccacttgt tccggctccc atataaatca gacgtccacc ttttttaaag
    25441 gactcaatcg tgttagcaat cactggttcg atacttgtca gttctttttc aaccgcagcg
    25501 gccactttct gatcttcttt gttcattagt tgaacggctt gagaaatact catctcatct
    25561 aatccaaaag tttcttggtt tctgcgttcc gttgttaggt tctctaaatt gattttgctc
    25621 atttttatct tccttctttt cttataaatt gatagttcat accagctttg atttggttga
    25681 tcaatggtag ctcttctttg ataactttag ctactacatt cactttttca tccgctggca
    25741 aatcatattt ggttaattgg atctcaccca tatagcgcaa atacttttcg ttatctaaag
    25801 taatagaccc tttcatacgc gtagctgtat ttcttgctgg gataggcgga atctctcgaa
    25861 aacgtgcatc tacgcttcgt atgacatctc ttgcatcgtc ttgccgattc gtatgcttcc
    25921 ctaatatata ttcgtaaaat tcttcatcta caggttccgt atggagcaag actgtctgtt
    25981 ctttttggaa agaagcaaat tgctcctgaa cgttttctga cagacctgca tctccgatgt
    26041 aaacaatatc cgtgttactt gctaatagat ctagagcgcc tgcaagagga tggatccctc
    26101 gatgttcttc caaagttggc aatccttgat atagtgggcc tctcaactct gtatctcctg
    26161 gtacaaaacc ttggacaagg agaccttgaa ttttgagcca ttgatttttt ttctgatacc
    26221 attctttgtc cagccctgtt tcgggacgtg gataataatt atgccaagct tcaatctgtg
    26281 agaaatcagc ttctgcttct ttcaattcat cgatatcttt aggcgtgatc gtgctagcat
    26341 tcaaactgat tttcaattga tgtgaccagt cggcaatctg ctgattggaa atatgataat
    26401 ccattcttaa acctgttaca ccaattgctt tcaaaggcgt tagatcttct aatgaaaaac
    26461 ccgctcgttc caatgcctct cctgaaatat cgaccatcaa ttctagttta ttctttttcg
    26521 cttcgcttcc tagttcgatc aatcgctttt gatatgccga aacatcatct tctggtatat
    26581 gcatggaagt gaaaataccg acaaagccat tggatgccat ttggcggata tactgtttct
    26641 tctcttctga caaagaatcg ttcataaata ttgaaaagcc aaacatatta tccctctttt
    26701 tctgtatccg tcttcatttc tatgactaga gtatatatct aaagaaacaa aatttcaata
    26761 cttttattaa aataattaaa tttcatttct aaaggttgag tttatattat caaaagaatt
    26821 gtcataacct aaaaaaggca ctgctaagca aaagcagtgc cttgaaatat tttatttaaa
    26881 taacacaaaa acataattac tcataagaaa ccaattctgt attatctacc atgaatgtaa
    26941 agaacccctc attaggcatc ggatgaaaat aagcattgat cactcttatc aaggcattat
    27001 ggcaaactaa cagatagaca ttttcttcgt cttcgatgca ttctttcaat aaaggaacga
    27061 tccgtgcata cacatctaag actgactccc catttgggaa tcgtacagcg aacgctaaac
    27121 gtgctttttg aaagttctca tctttgcttg gcagtccatc ataatcccca aaatccattt
    27181 ctaccaatcg ttcatcaacg gtcagcggca agctgagacg atcagcaact gcttgtgctg
    27241 tatctcttgc tcgttgaaga ggggaatgga tgattttcgt gattggaaca ggcaaatcta
    27301 ctaccttttc tgctagtttc tgtgcctgca tctgtccttt ttcagtcagc ggcacatctg
    27361 cccgtccgca aactaatcct tgtgcgttcc aagttgtttc tccgtgtctc gttacataaa
    27421 gcatgtgatt ctctccttcg ttggcaaagt tccccttaac cataccttgt tgttcttgag
    27481 attacaaggg ttagattctc tcaagagaaa taatctcagt agatacttta tccaggaagg
    27541 tttgatcatg ttccactagt aacatggcag gtttcacgtt caagatcagt tgttctaatt
    27601 gttcttgatt gaagacatcc aaataattca atggttcatc ccatgtatat agttcagctg
    27661 gctgtgataa agatttagcc aattccactt ttttccgttg gcccatactc atctgctcga
    27721 tcttgttatg aaaaacatct ctttccatcc caagctttcg gaggttgttc aaaaatgctt
    27781 ggtagtctac ttggtttttc tctgcaaatt ccgccaacgt tcctcgattg tcttcataat
    27841 tttggcttgc ataactaatg ctcagatgtt ttggctggta tttttctcct ataaccttgc
    27901 cgttgaatgc ccccagaaga taatggataa tggatgactt tcctgcaccg ttaggacctg
    27961 aaatcgccac ccgctgatga ggctcgattg taaaatgaat tggctcgaat aacagatttt
    28021 cataccctaa ttgaagatct tctacgctta aaagtcgctt atggtgagac gcttggctat
    28081 tcatcgtcaa agaatcgata tattcgatat cttttaatag tttttctttc tcgctgatct
    28141 gggtctccat ccgattcacg attgctttgg atcgtttcat cgtccgtgca gcatcagcac
    28201 ccactgctcc tttattcact cgtctagatt cagtatcgat gaatccgact tgtttctttg
    28261 ttttatctcc ttctcgggaa cgagaccatt cggctttttc agctgctgtt ttctttagcc
    28321 tactgacttc ttttttcaat ttttcatttt gagccatttc aaattcatca cgaagttttt
    28381 tctgttcttc atagatagag aaattccctt gataaagttc cagttgactt ttttcgattg
    28441 ctaaaacatg gtccactact tcatcgataa atccccggtc atggctgacc acgatgaagc
    28501 cttgtttttt ctttttcaaa taagccgcta cttgttttct accagagata tccaaatgat
    28561 tcgttggttc atcgattaac gggaaatgag tgtcatccac aaataaaagt gccagtagga
    28621 cttttgtctt ctctcccccc gatagtgtgc tgaattctct ccataagatt tcaggatctg
    28681 tttgcatcaa ttggagctct ctttcgattt cccatatctc aaaatccgta atatcattta
    28741 acacgtaata ggttaaacgt tctttatctt ttgtctgttg cgggaaatag gcaaattctt
    28801 gctgatggat tacttgccct tgataaggta gtttgttttg tagaatattc agtaaagttg
    28861 tctttcctcg accgtttcgt ccgataagtc ctagtttcca ttgtgtgtca aaatttagat
    28921 ttgcttgttc aaataataat gtgccttggc tgtcgtagcc gaatgtcaga ttttttattt
    28981 cgattttaga cattttccat cacctcgtaa aaaaagacca gtcctatttt cagaactggc
    29041 taaagaggat gcaagggttg gatacacaca aaaagaccca tacatcgaaa aaaccaagcc
    29101 aatcccgaaa atctgcacac tgcttccttg tctccacaag gtacagtctg aatgcagctt
    29161 tcatcaatcg acttaatttt tcaatgcacg gatccctcac tttgtcctat atttgaaatt
    29221 acgatagcat aaagaaaatg attttgtcaa ctacttcggg aggatggtga aaaatctgct
    29281 aagtgtctgt cctaagattt ttgttttttc taatgtttgc ctgtgttcca ttgcttcttc
    29341 taaacctatt ggacccaaat gaatacttaa gacaattggg agaaaatcag aaagtccttc
    29401 tagctcttgg tctctacttc cgcaaatagc gattattggt tttcgatatt tttttgcaag
    29461 cactgctaca ccgtatggaa cttttccgtg gatcgtttgc tgatcgatcc gcccttctcc
    29521 tgtaatgatg acatctgctt gttcgatctt tttttccaat ccagtgattt ggctgactaa
    29581 atcgaaacca gaaaccatct tcgctcccaa caaaaaaagg gctccaccaa taccgcctgc
    29641 tgctccactt cctggaatat catctaaatc cacctcccag aaatctttta tttgctgagc
    29701 aatctttcct gcttgtttat ctaagaaagc tagcgtcgta tctattcctc ctttttgtct
    29761 tccaaattgc caagcagctc cttgttgacc tgtatatgga ttggtaacat ctgcagcgat
    29821 aatcaatttt actttagaaa caagaacgtt tcttcgatca aaatgaagtt ttttagtaga
    29881 taataaagga tttccttctg tgtacccttc taaagaagca cccaaggatt ggagtaatcc
    29941 tagaccacca tcgctacacc cgcttcctcc taaagtgaca ataatttgtt ctacttcttg
    30001 aagcagtgca tctttaataa gttctcctaa ggcataactg gaagcaagtc ggatagtttg
    30061 atccgttggt ttatcaagta gatctaaccc caatgcttgt gcagattcga tgactgcggt
    30121 gttttttcct tgccaagttg tcaataaata gctgattttt cgctgacgga ataagagatc
    30181 catcccatca atggtacgcc atgttcccct gagcgcgtaa tagattgctt ctgcagtatt
    30241 ttctccgcca tctgcaatcg gcacattgat ttttcttcct ttacgatctt cccagccatt
    30301 tagaactgcc tgatttagtt caaatgaaga agcacttcct ttaaatgaat caattgccgc
    30361 caaaatatcc atctgtatcc ctcactttct tcttaagtaa gacacttttt ttcatttttt
    30421 tcaaacaaaa caatgctatt ttcttccatt tttctatata ctaaaaggga actcattgaa
    30481 taaaggagag aaaaaacatg aaaatcgcaa tagctggggc cggagcaatg ggcagccgaa
    30541 tcggattgat gctacatcaa agcggaaatg aggtcctctt gatcgatcgt tggcctgctc
    30601 acattgaagc cattcgtaca aatgggttga ttgctgactt taacggtaaa gaagtggtcg
    30661 caaaactgcc aatctattca ccagaagaaa tcatcgaaag caacgaacac gtggacttga
    30721 tcgtagcatt aacaaaagcc aatcaattag atgatatgtt ttgttcgatc caatccatta
    30781 ttactgacaa tacttatgtt ttgtgtttac taaatggatt aggtcatgaa gatgtactgg
    30841 aaaaatatgt gccaaagaaa aatatcttat ttgggatcac gatgtggaca gctggtctag
    30901 ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat cgaactagag aacttggaac
    30961 cagaaggcga agcatttaca aaaaaagttg tagaagtctt tcaagaagca aatttaaatc
    31021 cgatctatag ccataacgtt cgttattcca tttggcgaaa agcttgtgta aatggaacat
    31081 tgaatggttt atgcacgatt ttggattgta atattgctga actaggcgcg caaaaagcgg
    31141 cagagagcat ggttcgaaca atcgtatctg aatttgcttc tatcgcagca aaagaaggaa
    31201 tcatactaga tcaagaagaa gtgtaccaac atattgcttc tacttatgat cctgacaaca
    31261 tcgggttgca ttacccatct atgtaccaag acctgatcaa aaaccatcgt ttgactgaaa
    31321 tcgactacat caatggtgcc atttggcgaa aaggacaaaa atatgatatt gctacaccat
    31381 attgtgcctt tttgacccaa ttagtccatg caaaagaagg aatccttggc gcagaataaa
    31441 aggctctctg atgagaatca gctgaatcag cagctggttc tcttttttta agcccaataa
    31501 aagatcaaaa agaaaacaca actctcatga tgccataaga attgtgcttg tttgtttcag
    31561 tacataatct cttcaaaatg taggtttttt atttgttttc aacagaggag aggtcatatt
    31621 cgtattgctc ataaatagtg gaatcgttag tgataaatcg ttttgcccaa ttttctgcat
    31681 cataaaattc caatagctct gataggattg gcttaaatgg tgtatgcaga aattccggta
    31741 attcatctaa atcacgttct cgaagcaaaa aaatattttc aggacgataa aaatgataat
    31801 gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa atacatggat tcaaatgtcc
    31861 gcaaagtcac tccttgctga ccctcccgca acagttccag aatcccgcgc gattcccctg
    31921 tttttttcag gtactcttca taagtaagat atccaggttg ttcattccct cgatgtggaa
    31981 gaatgaaata gttggtcgta acatctcttt ttataaattc ctgcttatag ctttctgctc
    32041 gttcttttct cccattattc gttgcaccaa aaaatagatc cgtctttggt tcgctagaag
    32101 aaaaattcat atgtttcgaa taaaatgaac tattatgttt cagcccaagt tcttttgctt
    32161 ccaaaaaatc aaaatgataa atctcatcga ttgccggttc atcttgtgcc tgcttcagca
    32221 tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa cacaattttt gcattggtaa
    32281 atgggcgcac gcgtcgagcc atctccatat caaatccata atctaaaaaa acgatcagct
    32341 gataatttgc caactgttgt ctagctgatt taatgaattc cgactgtagt aaatcttttc
    32401 cggaccaaac atctaaagag cggatggaag gcaaaaaata aagtgattta ttttttgcaa
    32461 ctagcagtac gtcatttttg ttcatctttt ccgcctcact ttctctgtca ctttttccta
    32521 tgatagcctg tttaaatagt aaaaagccag taaaacgttt cctatatttg tatgaaaatt
    32581 taacatttgt atgaaaatat gatgaaataa atcattcttc tattaaaatt cgcctaaaag
    32641 ttttctgaat agttgtaatt gtctgaaaat ttgttatcat tagtcattgt taaatattgc
    32701 tatttattct taatataaat tttgattgtg aatttaaaag aaagaggtca tttatggaag
    32761 ataaaaaatt gaaaaaacaa cttagctcca gacatatcac catgttagct ttaggtggag
    32821 caatcggtgc aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg
    32881 tattgattgc ttttttgatt ggtgggctga ttttatatat cgtgatgaaa ggtctgggaa
    32941 aaatcgtttt aagcggtggc gacacgcatc acggattatc tggtttgata cgcccttatc
    33001 taggtgctca ttctgctgat tttactgatt gggtctactg gtctatgtgg attatcaata
    33061 tcatcgctga agctgttgca gctgcttcct ttttgcagtt gtggttcccg cacattccag
    33121 cctgggtatt tgtttttatg ttggctgttt taactacgat catcaatctt tattcggtac
    33181 ggttattcgc agaaacagaa tattggctgg cttttgctaa aatcagtgtc attatcttac
    33241 tgatcatttt tgctacttat cttgtcggtc agcaaatgtt gggcacaggc gttttcccta
    33301 cccttcagca attgactgac catggtgggt ttactcctca tggtatgaag ggaatcatca
    33361 gttctttgct tgtagttatc tattcttacg gcggttctga actgattgct atcacggtaa
    33421 gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat caaaggtgtc atgggaagaa
    33481 tcatttcctt ttacatcatt cctttgtttt tgcttttgat tctttttcct tggaatactt
    33541 tagcaggcac gaatgtcagt ccttttgtga tggtatttga aaaaatgaat atcccatttg
    33601 cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt ctcatctatc aattcgggtg
    33661 tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa
    33721 aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgttttattt tgtaccagtg
    33781 ttctttatct tggtgtagct ctttcttatt ttgtcggtga caaattattt ggctatctgg
    33841 caggttccct ttcgtataca gtattattga tctggatcat tatcagtgcg gcatcctttg
    33901 tactggcact aagaaaagga acattatggg ataaaggaat cagctttttt gcattagctg
    33961 tcttaggatt gattttcctt ggtatcctgt tcacgaactc cattggggta actgtattga
    34021 ctggcctgct ttacctgttc atttatttca gttatcaaaa aaagaatgat gcgttcgtat
    34081 tgacgaatga atgaaatcga tagaaaattt tattttattt atgtggaata gatggagcta
    34141 ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa taaacagtct
    34201 catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg attgttctcc
    34261 atgctcatga catgatagaa gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag
    34321 tattggaagt ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac
    34381 ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta cacgagtatt catggcgttg
    34441 atgatgggtg cagtcatggc agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac
    34501 aaaaagataa atgttatgat cttagccgct agtcttcttg tttttgccgg ttccttgttt
    34561 atggtacgaa gccaaactac tgtcaacgac acagcttgga tgaaggccat gattcctcat
    34621 cattctatcg ctatcctggc aagtaaacga gctgatttaa aagatcctga agtaaaaaaa
    34681 ttagcggaag aaatcattca ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc
    34741 aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag aggatgttcc agaagttatc
    34801 agcttcaaga aataagaaga agctacttct ggaacgccgt gaataagggg ttgtgaaaaa
    34861 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcct atttttcgtt
    34921 tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattaag aggttgtaaa
    34981 atcagcgttc tgacctgagt attagagaga caaatggaaa aatagttcct catattttgc
    35041 cattttttga gctaataccg agggtcagct gattgaacac cgtttatcga gaggttgtga
    35101 aaaagtcgct tagtttcgcg tattaagaaa aattttgtga aatggctttc cacatttttg
    35161 tataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttattc gtaaaaggga
    35221 ttgtgacaaa agtcttgtca caatcccttt ttatgttttt attgttctaa ttgcgctgtc
    35281 ttgcttactg cttcttgcgg tttcacaaat ttttttgaga ttccccaacc tgtgaaggca
    35341 ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata cgaatggcaa gtaagtgagt
    35401 gtaggaatac caaatgtact tgctacgaaa cttccggcaa ttccccaagg cactaagtag
    35461 ttgattactg ttccaccatc ttctagcgtt ctacctaata cagttggatc aaatcctgct
    35521 ttttcataag tttctttaaa tgcattacca ggcagaataa cagataggaa ttgttcacca
    35581 ataaaaatat ttactccgat acttgtcatc aatgttgtaa aaatcaaatt tggtgtgttc
    35641 gtcatctttc ttgatactcg ttccatcacg gttgagatca agccaaattc gatcaacaat
    35701 ccacctaatg acaaagttag aataatcaat gacacagtag gcatcatact ttcgattcct
    35761 ccgcggctaa gcagtgaatc gatttgttgg tttccagtat ttgaaacaaa ccctgattcg
    35821 atgaccccag ccaacttcgt taaggaagtt tgtgggtttt gaataaaaat cattcctact
    35881 gctacagcga tattcaacaa aatcgttaaa attgccggca cctttttcca tgcacacagt
    35941 aacattaaaa ataaaggaat cagtgaccag attgaaatag taaagtttgc ttctagaaca
    36001 tcagtcactt cacgaataga tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg
    36061 aataaaaaga aagaaacaac aaatgccggg acagttgacc acatcatatg cttgatgtgt
    36121 ttgaacaaat cggcatcaac aatagctgct gctagatttg ttgattcgga taatggagac
    36181 atcttatctc caaaaacggc acctgaaaca atagctccaa caaccagcgc tggatttaag
    36241 cctagtgttg tcccaatacc gaaaaatgca atcccaatcg ttgacataac agtaaaggca
    36301 ctccctacag cgctacctac gattgcacac acaacaaata cagaagggac aaaccactgg
    36361 acactgatca atttgaaccc aatgaccatt agagttggaa tgatccctgc ttgtatccat
    36421 acagcaatca atgaaccgac taaaataaaa ataaagattg ggatgattcc tggtttgatg
    36481 ccattgatga ttccttcgtg cagccaatct gtcgggtatc tttttatcac tgcatacaac
    36541 atgaccattc cgatcgctgc taagatcgtg atattaggtg agacctttaa tccgacaacg
    36601 ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg ttgcttctaa aaaacttggt
    36661 gttccttttt ttcccatgat aatctctcct tttttagttg ttcttatgtt ctaaaaaagt
    36721 taagatatcg gattactccg ccgcaattca tccgcgattc ctcctttata aaaataaaaa
    36781 gacctgtttt aaactaagga cgaatgactc cgcggtacca ccttaattaa aaacagatct
    36841 gtttttcact ttcaaacttt tattcaaatg tatggacttt ccatgtaatt cgtcaattag
    36901 gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa acacctaacc gctactgcgt
    36961 gaaaagttga cttgtacgaa gtttaccttc cataagatca tttgtcaatg aaaaagaaaa
    37021 tattttttca tttttctgaa aattattttt ataactttca cacacaagat tattttttac
    37081 tagaaagtag cgccctcttg tttttttctt gctactattt cttgatgttc ttgcttgatt
    37141 ctttctaata gctttggctc tttcaacaga tcaagagctg tattggcgag tagttctgct
    37201 gcaatcgcaa tagaagcaag gcctttgtca cttttagcag cagctttgaa ttcttctgtg
    37261 tgtccggcaa tatatgtctc agagattgct accgtaggct ggattgttgg tatgacttgg
    37321 ctgacatttc ctacatcaga agaaccgaga cttgttcttt gttccgtttc tatttcttca
    37381 tctggaacac ctgcttcttt tgcatgctgg aagaataagt catcaaaaga aggagtaacg
    37441 atgacatcat caactgcatt ttggaacaat ccaaactcaa aagtcgtgcc cgtacttatc
    37501 gcagcacctc gtacgatatt ttccactttt tcatatactt tatctaatgt ttgccgattt
    37561 cctgcacgta aatagaaacg tccagcagcg taatctggaa tgacgttggc agctactcct
    37621 ccgtctgtaa tgatcccatg gatattgaca tcttttggca attgcaagcg tagagcgttg
    37681 attccattaa acacttggat caatgcttcc aaagcattta ctcctttttc aggtgctgct
    37741 gctgcatggg aagcgacacc gtaaaatttg atgtcgactg gatcattcgc taaagattct
    37801 gtcgttttcc cataacgaaa agctggatgg acgcataacg ctacatcgac atcctcaaaa
    37861 aaaccttctc gaacaaagct gcctttagct gatccgttct cgcccccttc ttcccctggg
    37921 gtgccatata cacggatttc tccaccagtt tcatcaatga attgcttcac tatgctggcg
    37981 gctaatacgg agtaagtacc aaacaaatta tgcccgcatg catgaccaat acctggtaaa
    38041 gcatcgaatt ctgccaaaaa ggcaattgtg ggaccgggtt tattggaaat ataacgtgca
    38101 tcaaaggctg ttcggtgtcc agctacttct ttttctaccc aaaacctttc tttttctaat
    38161 tgtttaatca acgctgctga ggaataaaac tcatagttac ttacttcagg atgttcgtgg
    38221 atatctagag caagtgcttg atattctggt aaacgatatt ggattagatc cttcaattgg
    38281 tttgttgaga ttgttgttgt cataaaaaat tcctcctatg ctagtcattt ttcttatttt
    38341 ctcccgaatg tttcccatgc aggcacgctt gatccttttg atgttttctc gattactttg
    38401 actgtgtctt ctgtttgata agcgtctact attttttgat acgttttatt gttttcatct
    38461 tcttttcttg caacgatgat attcacataa ggacgtgcat ggtcatccac tggttctaag
    38521 aagatggcat cagaagcagg attaaatcca gcatctacag ctacaccgct attgatcact
    38581 gaaatgtcta catctgacaa agcacgtgct gtttgggaag catctaattc agaaatgtct
    38641 agattttttc tattctcggt aatatcagaa acagtgggcg cttgtccttt tgccttatcc
    38701 actttgatca atccagcagt ttgcaataac agaagagctc tgccgccatt tgtaacatca
    38761 ttagggattg ctacttttgc tccgtctttg atatcagcta catccttcaa agtgttagaa
    38821 taaattccca gcggtgcatt gacagtattt ccgatcgata ctaaatcagt tccgtgttct
    38881 ttattgtaat tgtctaaaaa gaattgatgt tggaaagaat tcaaatcaat cgatccatct
    38941 gctaaagcca catttggttg attgtaatct gtaaatttca cgtattccaa attaatgttc
    39001 tcctttttca aacggtcttt cacgttgtcc catacatctg tatcttctcc aataatccct
    39061 agttttacga tagtttcttt tttgtcagtt gtatctgctt ttccttggct acaccctgcc
    39121 aaccaaataa ttgctacaaa tgtaatgact acaaaacgaa taattttttt cattttttct
    39181 cctccacttt caatgtgtaa tttttttaat cagttgatta ctgataaatt gacttaaaaa
    39241 aactaagatt agaatcaata atgtagccac aatcgttaca tctgtttgga atcgattata
    39301 ccctctcgat atagcaagat tccctaatcc gcctgcacct actgctccag ccatggtagt
    39361 cagtccgatc acgttgatga tagtcaacgc cgagacccga atgataccag gcaaaccttc
    39421 taacaaatac acacgtaaaa tgataccgat tggactggtt cccatcgctt ctgccgcttc
    39481 gatcacccca ggatcaactt ctagcagtgc gttctcgatt tgacgagcaa aaaacggaac
    39541 aactccaaaa atcaatggca ctaaagctgc tttcgcacca atcgtcgttc caactaagaa
    39601 acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa gggatggaac gaatgatatt
    39661 gatcagctta tcaagtattt caaatagcag acggttagct agtaatccat tagggcgtgt
    39721 cactactaac gtcacaccta agatgattcc aatcatcccg ccaatgaatg ctgtccaaaa
    39781 agacatatat agtgtttgct tcgtcgcttc gacaaattca tccggaatca aataaacatt
    39841 gggcaaatat gttttaaaga aatcagtcat tttatcttcc tccttcgatc actttgatac
    39901 gactatctaa atcgtttttt tgtaaaatat ttatttttac accagatttg atcaaataat
    39961 ccaatgcttt tttccgctgt ttggtttctc cagctaaagt aacgatcaaa ctccctaacg
    40021 gcgtttcttg taataactct acattgccat ataaaatatt cgctgttact tgatatttgc
    40081 tgtacagatg agcgattaga ggttcgttcg tctgatttcc aatataactt aattccacca
    40141 gccatttatt ggcgatctga tcggcaaaag cagaatgggc aatgatcgtt tctaatgctt
    40201 ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa ttcttcttca ggattgctga
    40261 agatctggat gctgctacct ttttcaacga ttcttccatc ttccataacc gctaccttgt
    40321 tgcagatttc tttgacaact tgcatctcat gagtgatcag gacgattgtt aaacctaact
    40381 ggcgattcaa tttttttaac aatgcaagga tctgaagggt cgttttcgga tcaagtgcac
    40441 tggttgcttc atcacataat aataccttag gttcattcgc caaagctcgt gcgattgcta
    40501 ctcgttgttt ttgtcccccc gacagttgtt taggataagc agatgctttt tcttccaaac
    40561 cgacaagttc cagtagttca ttcacttttt gacgacgttc ctgtttcgat tttccagaat
    40621 actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct ttccattaga ttgaaatgct
    40681 gaaagatcat accgatggcc tttctttctt ttcgcaattc tttgggagag agctgactca
    40741 gattcgtctg attgacaatg acttctcctg ctgttggttt ctgcagcaaa ttcatcacac
    40801 gaactaacgt acttttccct gcacctgagt aaccgacgat tccataaaca tcgccttttt
    40861 caatcgttaa atctacagaa tctactgcct gtatctcttt ttctttttgt ttgaaacata
    40921 cagaaacatc tcgcaattca atcattaaaa ttcctcctta tctaaaaaaa gcgtcctcaa
    40981 gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg tgttaccacc tcatgttcaa
    41041 gctgtgttca cacacaactc ctcttcaagt acgatcatac tctagcaaga tatcggtcgc
    41101 agccgtcatc gccttacatc tatgctcggg atgaattgct cgatggccat tttccaatag
    41161 tcgttccttg cttcttctca gctaccgaag ctctctgtca aaggttcttc tatcgtactt
    41221 accatttcaa tgcaaataat attcaaaaaa aaagtccttt tactaacata aactatgtca
    41281 gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa gtttccttcc ctcaagccca
    41341 acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg aacagttccg aatttgccta
    41401 cttatcgaca aattattctc caaggccatt ttcagctttt cttacttgac tcttctcacc
    41461 agccagagcc tctctgcaaa gtttccaaac ttactttcct tttcaacgaa atcgatattt
    41521 tgttgttggg attaatctac atgaggaaaa ctgaaaagtc aagtacgaaa ttcaaattat
    41581 tttttctata tttatttttc tgttttttta tttaacattt caataacagt tcgaagatct
    41641 tttccttccg caagaagttt caatttttcc ttttcttcga cggtataatt tcggacaaaa
    41701 ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg aagcagctgt tgctcctact
    41761 aaagaatctt ccactgcaaa ggctgaacta gcttctaatc ctgttcgttc caaagctgtc
    41821 agataaggaa tcggtgaagg cttgttctcc gacacttcgt ctccgaatgt aaacgtgtcc
    41881 aagtaatcag ctaatttaaa gtgatccaag atatctgttg ctcttttcgt aacagtggtc
    41941 gtagcaatgc ctagtttcat atgacgttct tttaagtctg ccaatatttc ttttgcatat
    42001 ggttttaagt ggatggctcc gcttgtcagc tgttgataaa aatattcttc acgcttcgct
    42061 cggacttcct gtactttttc agggctacct gctgctttca ctaaaatatc aaaactttgt
    42121 ttccagtttt ttccgcccca agcgtcaacg atttcttttg ggatatggat atcaaattgc
    42181 tgaaatcccc aaagccagcc atcacgatac gttttttctg tgtcaactaa caaaccatct
    42241 aaatcaaata ttgcaccttt catctataca ttaatccttt ccaaaacaat acacatagtt
    42301 tagcacgact ggtccaaaaa gaacagaagc aaagaaccag ccttctcgtt tataagagtt
    42361 cttggaatcg acggatatag attttcttca cagctgtcac aagcgccaga taagcgatga
    42421 ctgtagcaaa taaccataac cagtaattgc caggaagtgg catcaagcct aattcagcac
    42481 caaaacttgt aaaaggaagg acagaaccga caataattcc taatgtagtg ataacaaaca
    42541 tggcaaatgt tgcgttgctt tggataaatg gtactttcgg tgtacgcagt gcatggagca
    42601 ctaaggtttg tgtccatagt gattcgacaa accatcctgc atggaataat gcgatgaaaa
    42661 tcactcgctg tgaactatcc aatgtatgga aacttccgcc cactacagca ggacaaataa
    42721 caaaatacat caacaagtaa gtagtaatat caaatatcga acttgttgga ccaaagtaaa
    42781 ccatgaattc cccaatacta gatgcattcc atttttttgg ctcgtccaaa tattctttat
    42841 ccatccgatc ccatggcaag gaaatgcagg aaacatcata gatcagattc aaaaacaaaa
    42901 gttgtaaagg cagcattggt aaaaatggca ggaacgtact tgcaatcagc acggaaaaca
    42961 tattgccaaa attagaactt gctgtagcct tgatatattt catgatattc ccaaaagttt
    43021 ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa aggatcacgt
    43081 ctgcagattc tttcgcaata tccacagctg tatctacaga aataccgata tcagccactt
    43141 tcattgcagg agcgtcattg ataccatctc ctaaaaatcc gacagtatga ccatttttac
    43201 gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa ttttacgaat acttcatggt
    43261 cttcagctac ttgtgctaat tcatgatcac tcatttccga gattttctca ccggtaacca
    43321 attcattgat ctctagacct acttgacggc acactgaacg tgtaactaat tcgttgtccc
    43381 cagtcagaac ttttacagct acaccatgtt ctttcagagc tttcaacgct tgtttcgttg
    43441 tttcttttgg tggatcaagg aaagcaagat aaccaatcaa aaccatgtct gactcatctt
    43501 tgactgaaaa ttctccaacg acgcttggat tggtcttttg tgcgacacca atcactcgta
    43561 agccatcttc attaagtgac tgtacttttg caagtacggc ttcttttcgt tcttttgtta
    43621 atggtgtaac agtaccgcca agatcaataa atttagaaat gttcagcatt tcttcaatcg
    43681 caccttttgt gatcatttgt gttttaccag ctggatcagc aaccacaaca ctcatacgac
    43741 gacgttcaaa atcaaaagga atttcatcca ctttttgata attgatctta tctgtatcta
    43801 aagtttctgt tgcttcatca atgatagcca catctaacaa atttttcagc cctgtttgat
    43861 agtagctatt tagatacgca tggcgcaaaa cacgatcatc ttcatttcct tcacaatcca
    43921 tatgatattc aagaatgatt ttatcttgtg tcaatgtccc cgttttatcc gtacataaca
    43981 catcgattgc accaaagttc tggatggcat tcaaattttt gatcactgtt ccttttttgg
    44041 ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc atttcaggtg
    44101 ttagaccaac tgctacagat agtccaaaaa gaaaagcttc tagccaatcc ccttttgtaa
    44161 gcccattgat gacgataact gctggagcca tcaatgacat aaatttgatc agtagcatgg
    44221 atgttttatg gatgcctaat tcaaagcttg attgaattgg tttttcagat agcgtttctg
    44281 ctacatgacc aaatagtgtt tcatttccaa cagaaacaat gattccttcc gcacttccac
    44341 tgatgacatt acttcccata aagacaagat tttcgtaact tgtttcgctt tgctgatcgt
    44401 cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat gctgcttggg
    44461 aaatgaataa atcctttgcg gacaataaac gcatatccgc tggtatcata tcccctgctg
    44521 aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgtg tactttccat
    44581 tacgttttac agctgccgtt actttcacaa gtgatttcag tttttcagcc gcttgattgg
    44641 aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat aaccatagtg aagataatgg
    44701 ctacaccgaa aagatcttta tctccaggag ctgctaatac atattccgtg atgaatgaaa
    44761 taattcctaa tgcgatcaac actaatgtaa atggcgtgat gtatgctttc aatatttcta
    44821 caatgaatgg tgtttttttg ccatatgaaa tcgcattttc accgtaattt tctcttgaac
    44881 gttcgatttg ttcttctgtc aatccatttg ctgatgcatg gaatagttca aataattctt
    44941 gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt ttttccatct
    45001 caatctctcc tttgtgtcca aaagacagct gttctcgctg cttttggtct ttagctattc
    45061 atcggatcga tagagaaaaa actgaaaggg gctgagaaca gatagataat agccctggcc
    45121 tttcctctag atccttgttt ttcgaatgac tgtcatccat ttgttctcat ctcctttact
    45181 aagtaatttt aattcatgac ttccaatcat ttttcgaata gttatttttc caattagacc
    45241 acctcctttt tatttttcac cgatggggat ttgcggcaac aaaaaaacct ctcgccaaat
    45301 actcagcgaa aggtttgaaa aagcatatta aaaaaagctt ctcagatctc atcgttgagt
    45361 tttgacactg tacaacgtaa aaagcagata cctgctttta gctaccttat gaaaagcctt
    45421 atctcgacaa ttcctgttct acccattggc gtctctcgac gtttttgggc agtagcctat
    45481 gtttgtatag gagcctcacc taacaaatct tattcggttt tccttcgtta atataacaac
    45541 taaaaaagag gatgtcaaat caattttcca tgtttttcat acttttttta aaatacacac
    45601 gttatattcc tgtctcacgc ggttttattt gtctcatttt ccacctcccg ctataataga
    45661 aatatagtag gaaacgaggt gctgttatgg aaagaacact tgttattatc aagccagacg
    45721 gcgtgagacg ccatttagtc ggaagcatca tccaacgttt tgaagcaaag ggactggcca
    45781 ttgctgaaat gaagtttgaa actatgactc ctgaattagc aaaagaacat tatcagcact
    45841 tgactgagcg cagttttttt gatgaactga tcgattacat gacttccggt cctgtcgttt
    45901 atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg taagatggtt ggagcaacaa
    45961 aagctgccga tgctgttcca ggtacgatcc gaggagacta cgcgctgccg gggactgaaa
    46021 acatcattca cgcttctgat tcccgtgatg ctgcggtaaa agagatcgcc cgctttttcc
    46081 cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa gtgatacaaa ttctaaactg
    46141 ctggtctagt tgctgggttc agcaatggcc agcagttttt tctctaagga cctccgttga
    46201 ttctttccta acatatattc gagcactgtg aagaattctt ggtagtcttt cgtcttttct
    46261 tcccctaaag cagcagaaat caatctttga taataatctg ccatcaatgt ttcatagatc
    46321 actgtacttc tttctttatg tgctaagctc cgtttcatat cttcattcac actacgtgcc
    46381 tttttcaacc gaccattttc tatataaaac gaagtcaggt tcattaaaaa agtaaagaga
    46441 tactgctcat gattgttcaa ctgtgctaat cgtcgcattc tttttttgat tttcaaataa
    46501 ttgttctcaa tgaaccgatc actgaatatc caaggaaaat cagctagaat agatagttca
    46561 aattgaccat agtctggtat ctgcatcaag tattcttcaa tgattcggca ttcataacgt
    46621 aaaaattcaa attctgtctc agatacttct gttttttcga ccatgtgaag attcaaaacc
    46681 aaataataca gatcttctgt tttcttaaaa tgcctacggt tattgctgat ctctttataa
    46741 ggatcaaagc tgtcttcttg tacccgatct agcattcgtc tttttttctt taactttatt
    46801 ggttcatcca gctggtttcg catccattca tattctgcaa gtgtcatgtt cattcgctct
    46861 aaaaatgatc tcaatgttac aaatgacatt tttttcactg aatttgtctc aatcccgact
    46921 agagaagtct taaaacagat tccttccgct aattgggact gtgtcaactt cttgttgact
    46981 cgaagtttct tgatcaattc accatcatac ataatagttc tcctttttaa tttaaatttt
    47041 tccacattca ataaatagat acgttaaaaa ggaaaaatac acgaaaaaaa taaaaaaatg
    47101 tttttttcat aaaaaatacc aaccttcaag cattagaatg ttgtctaact actgaaggtt
    47161 ggtttgattg tttattgatt atttttttgc tgttcaacgt ctgttcttgt tactggatat
    47221 aagttggttg ttgttttatt accatttttt gaaaagacac ttgtcgaatg ttcgcctttt
    47281 tgcttttcga ttgcttcatt acgttccgct tcatttttat agtcatattt tttcggatca
    47341 atcgtctcaa atccgtttgg cacataaaac cgcaagagat tttcctgatt cagcttatct
    47401 gaaagactta acgcagtctt gactttttcc tgatctttgg agatttcctc atcgattcct
    47461 gctgctttcg tatcgatgat ttctcctgtc tgattattgt aagcttttcc tcctaggacg
    47521 gtgtatttgg gtgtcacaaa gttcccattt cgaaaagcaa ctacttgatc atgctgagaa
    47581 gataataagt ctgtaccaaa atgaagataa tctttatcat cgattcccaa tagatgtagt
    47641 aatgtcggca aaacatcgat ttctccaccg tattcatgat tgactgtccc tttagtataa
    47701 ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa ttcatcccat
    47761 gtatctggat ctttaccaag agcgcttgct aaatctttat tatctgtatt cgagatccca
    47821 aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa taccagattt tttcaaatac
    47881 gtaaaaaatt gttccaatga ttgatccaga taatgtgccg ttcgaacata attatctact
    47941 gtgctattgc cagtattcaa agatggaaag tcgaaattct tgtcatccgt ataataaggc
    48001 gtgtggttgg ttacggaaag aaatttcgtg taaaacggct gttgtagatg ctcaagatat
    48061 tgggcagatt ctctgaataa atctttatct aatatcccat aacctaacgt ttcgtcggat
    48121 tcatcaaaat aagatcgatc aaagaaattg tcataaccta agtttttata tacatgatct
    48181 ctattccaaa aactagctac attcccatgg aaaacagcgc ttgtatagcc ttgcttttgt
    48241 ccaaggatcg ctggtgcggc ttgaaagaca ttgtctgacc caagttctgt gaataatgag
    48301 ccttgaggta aaccaaatgt gccagtttcc aacatatttt cagcatcact tgtctttccc
    48361 tgtcctactt catgaaagaa attgtcaaag ctgatcgttg cttgattctg gaaaatacta
    48421 tttaaaaacg gtgtgacttc ttgtccgtct accttcatat taatcaaaaa ctgctggaaa
    48481 ctttctaaat ggagaacgat cacatttttc cctttagcga tcccaaatgt ttcgggattt
    48541 ggctctgcat aatgtttctt tgtataatct aaaacttcat caatcccatt actgcttgca
    48601 tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt cataaatagt atacgcatcg
    48661 attcctagat atttgacaat atatgaacgg tcaaatgttc gctgcagcaa ttgtggacgg
    48721 ttggcttcgc tcagcgctag atttcctgaa aaaataagcc ctgaaagaca ggtaacagcg
    48781 atagccatcg gtttgccgac agggttgctt ttgataggta ttttcttgac cttcaaccaa
    48841 accagtagcc caatgaacac agcgatatcc agccagtaga tgacatcatg gggcttcatc
    48901 aaagaaaagg agcttcctga caatccttgc gacactttcg aaaagcctaa tacagatttg
    48961 actgtaatga aatctgtgaa ttcccgataa aaaataatat tcaagtacaa caaaagcgta
    49021 tttaaaatat caatgatcaa aagaatagct aacgctggct ttggcttttt aatatacaaa
    49081 gaaaggccaa aaaacaataa ggtagtcgca attgggttga tccacaagat aaagtactgg
    49141 atcgtacctt ccacacctaa tgaaaaatct gcatagtagg caatgatcgt cttcagccag
    49201 aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt tttaagttgt
    49261 gtcacgtata tcacttctct ctattcacat tttttcttat tttgctcctg aaaaatgaaa
    49321 atttcctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat tattccgtcc
    49381 acttttcaca gcataaaacc gctgcgatgt aagcgattaa actaatgaag agataaaagt
    49441 cttgtggtga ccgcttcact ccacggtatt caaaagccag tttctcggta ttcgtacaaa
    49501 aaaagaggct gtgacaaaat tcttgtcgca gctccttatt tcttgatgca ggacagcttt
    49561 ttcacaactt cttccttaaa cggtgttctc ctgccaactc ctcggccaca agtcacaata
    49621 tttataaaac atgagaagct gttttttgaa attgttcctt gtggctcaga gttaaacggc
    49681 aggctcaaga cctcttgata cacggtgttc aatcagctga ccctcggtat tagattatgt
    49741 caggacgcta attttacaac ctcttggtaa acggtgttca aaagcttgca tctgcggtaa
    49801 taagcccaac caaacgaaaa atataaggag ctattttttg aaattgctcc ttgtggctca
    49861 gagttaaacg gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccttcggt
    49921 attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc
    49981 aggtcaaaac gctgatttca caacctctgt ttttcattgt ccataggatt gatattgtcc
    50041 ttgtgatgac tcattcgtaa taattgaggc ttgattttct tcacttcccg ttatcgtttg
    50101 tccactgcca gaatctgaag gacttgttcc tgtcgactcg tttttttgag aagagacagt
    50161 tgttttcttg acttgttcat ttgtcttttt ccattccgct aaacgatcgg cgaccacttt
    50221 tttgtctact ttttcagaat aagcggtgat cacttttcca tttttcaaac gggctacttt
    50281 gtcgccatca tgagtcaaaa gcaaaacggt caaatgcttg tctttaaatt gatattgtct
    50341 gagagaaatc gcactaagta cgtcatcgat attaataaat tgattttctt cttttccttt
    50401 tggcaaataa atgtccaatt cgcctttttc gtaattggca gttattttat actccgccca
    50461 ccaataagtt gagaattctt ttttgaaatt atctactgat tctttgcttg ctacgatttt
    50521 ttctgtttct ttctgagcaa gatctgctgt catttttcct gaaaatgctg cggataaaat
    50581 aagcagtaag aaaaaaccga ataaccaaaa gagaagaaac ggtctttttt tcctcatgct
    50641 ttcctccact cctatttaat tttgactatt ctattatata caaaaaatga aatagaaaga
    50701 caaaaaagtt gaaaaagatc gtaaataatt gaacttcttt ttcacaaaat ggtaagaatg
    50761 gagaatttca cgcatttgct ctcaactatt ctttcttaag cctcaaatcc gtttttatct
    50821 gatacgtctt tgaaccaatg acccgatttt ttgattgttc tttttccatc ttgtgctaag
    50881 tctactgaaa taaagccata tcgattctta tatgcattgg tccacgacca gttatccata
    50941 catgtccaca tatgatagcc taaacaattg gcaccttctt gaatggcttg atggacatat
    51001 ttcagatgag attggacaaa ttcgatccga taatcatcgt ggatcatgcc atcttctttg
    51061 ataaatcgtg cttcgttttc tacacccata ccgttttcag aaataaagca gtcgatattt
    51121 ccataatgat ctcgtacatt gatcaaaata tcatagattc cttgttcata gatctcccat
    51181 ccgcgataag gattcatttt acgaccaggc atttcataat tgtcgaaata atcctcaggc
    51241 attggaccgt ttttagtatc gattggtgtt tcttttgctt ttacccggcg tggctggtaa
    51301 taattgatac ctaaaagatc tactgtattt tctttgataa ccgtcaaatc ctctgcatgg
    51361 atcacaggca tgagatcaag ttcttttact atctcaatca attcacttgg aaactcgcct
    51421 tttacagctg gatcaaggaa tgaacgattg aagaacgcat ctgctatttg cgctgcttta
    51481 acatcttcag ggtttgattc gtctcttgga tagcttggtg tcagattcaa gatgatacca
    51541 ataacaccac cttgctccat ttcatggtaa gatttgatgg ctaacgctga agccaacgct
    51601 tcatgatagc caacttggat cgcttttttc atatctgact cttctggata atgccatcga
    51661 tataaataac ctgcttcgac tggaacgatt ggttcattat gagtaaacca tttttttact
    51721 ttcgaaccaa acaattcaaa acaacttttg gcatattttt cataggcttc aaccgtttct
    51781 cggttcaacc atccgccttt ttcttgcaga tacatcggca tatcgaaatg atacagattc
    51841 acaaagggtt cgataccgtt ttcaatcaac tcatcaaaat aacgatgata gaaacgaacg
    51901 gcttcttcgt tgattgttcc agattcatca ggtatcaaac ggctccattg gatagatgtt
    51961 cgaaatgaat tatgcccagt ctgtttcatc aactgtacat cctcttgata cttcgtataa
    52021 acttgcgagg ttttttcagg tcctacttga tcaaagaact tttgcggttc ttttttgtac
    52081 caataatcaa aaatgttctc tccttttcca tcgcctgaaa tccttccttc tgtttgtggg
    52141 ccacttgctg cagaacccca ccaaaaattt gctggaaatt gatatttttt catcaaagca
    52201 cctctttttc ttttttagtt cgttttagtt gattccatgt atgatatttt ccatcacaat
    52261 cactttttca ttttcttcct cttctttttt gattcgtttt aacagctgtt ctgctgctcg
    52321 ttccccgatt ttatctgttg gtggcgtgat cgtgattttc ggaaggatac tcagatcgtt
    52381 tttatctaaa taatcatagt gactgaacgc aatatccttc ccgattctga caccttcttg
    52441 attgaaacat tcgatggcac caatcgacat attgtaattg ctgacaaaaa tagcagttgc
    52501 ccctttttct aataatgctt ttgccccgta atagccgctt tttctagaat agtctcctgt
    52561 aaagacctct aattggatcg atggatcatt ttggaccgct tctttggctc ctgctaaacg
    52621 ttctcttgct acgtagtctg tttgagtggc agcaataaat ccgatttttt tatgtccttg
    52681 ttcaccatga tgagcgatca cttttgctac actttctcga tcattcacta atattgtatc
    52741 aatacctgct ggtccttctg gacaattcaa tgcgataact ggaatactca tttttgtgag
    52801 catttcaaat ccagaccact cttcttcgga taagaagaca atcagcccat cgatcacatg
    52861 cttcatcagg tattcaattt tttgttcaat tttttctggc tgatcggcaa acccgcttaa
    52921 taataagcta tacccatttt cttcagctac tctttcgatc cctgagacaa cttccgaccc
    52981 aaaacggctg gaaatcccgt ttatcaaaag tccaatagag aagcttcgat tattcttcaa
    53041 ccctttagca atgatgtttt ctttatagtc caacgctgaa atcgcttctg ctattttttt
    53101 catgttcttt tccttcagtt gctgaccatt caagtaacgg gaaactgttc ccactgaaac
    53161 accagccatc ttagccacat cacggatcga tgccatacat tctcactcct gccagttaca
    53221 tttgatttct ctttcttgtt gttcgattat gatgaccgca ttctttgcat agtgttgctt
    53281 gtcatcaata tccatataac taaagtcttc agtagagcaa cttcctctca ctattaatta
    53341 tacttaaaca agtatttgaa ccggttcatt tttaatatag cattttttta aaagattgca
    53401 acactttttt aaaacgcttt ctgaaagtgg cggatttttt gcctttttaa aattttattc
    53461 taaaatttac tctatttgtg ggtgttttgt gcgtttttct cttccatccg tttgaagttt
    53521 tacattccaa tggtataatt tctttatcct atcaaatggg ggaagtattt tgaagaaatt
    53581 aatcgtacta tttattttgt ctgcgggact tttgacattg actggctgtg atttgtttgg
    53641 cggctctgac caatcctcgg acgaaacaac cgattcttct gtcttaaaag cggaaaaagc
    53701 agataccacc aacagtgtat atacgctaaa agtacgtaaa gatatggatt tatccgcggg
    53761 acagttgaca tttacagata aaaaaatgga atggacacgc acgtatcaga cacaaggaac
    53821 tgattcttct gaatcatctg aagaaaaaac agtgctgaca gatatcaaga tcactaccaa
    53881 agatgacgaa tatattattt caggaaaaga aaataaaaaa gaaacaacag tcatatttac
    53941 aaaaatcggt aattaccgtt tgaaggatga ggatggcaac atctacagtc tatagtgccg
    54001 tctgttggat cttcttttgt ttctcccctt cttttctgct atagtaaaga agcaagatga
    54061 ttgtaaagga gacgaaatgg atgatcgata gtcctgatta ttgtacatgt aataacaaca
    54121 aaaatatcca tacagaattt aatgaccaag gaagcttgag catatgcgac aactgtcaca
    54181 aactagtcaa agattcattt aaaacatttg aatcttatcc tgttcaaaat tatgtaaaaa
    54241 gtagaagaag ataaacaaat ccaagcaaaa ctcactatgg ctgattttta tgaacttcaa
    54301 actcagcttc ttcctttgat caagcttcat cagtaaaaca aacagtggat catttcagtt
    54361 tacaaatcaa agagtaaaga tttatttatt tcaaatattt gaatataaaa taaccaaacg
    54421 atagaaaggc ttattctaca tcacgtagta att
//
LOCUS       DAYYBG010000009        54186 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000009 DAYYBG010000000
VERSION     DAYYBG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.009"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..1423)
                     /gene="sufB"
                     /locus_tag="ACHAUN_000520"
     CDS             complement(32..1423)
                     /gene="sufB"
                     /locus_tag="ACHAUN_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8459250.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(1428..1898)
                     /gene="sufU"
                     /locus_tag="ACHAUN_000521"
     CDS             complement(1428..1898)
                     /gene="sufU"
                     /locus_tag="ACHAUN_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8459251.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(1885..3120)
                     /locus_tag="ACHAUN_000522"
     CDS             complement(1885..3120)
                     /locus_tag="ACHAUN_000522"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8459252.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="ACHAUN_000523"
     CDS             complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="ACHAUN_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8459253.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="ACHAUN_000524"
     CDS             complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="ACHAUN_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8459254.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(5386..6234)
                     /locus_tag="ACHAUN_000525"
     CDS             complement(5386..6234)
                     /locus_tag="ACHAUN_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8459255.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(6258..6944)
                     /locus_tag="ACHAUN_000526"
     CDS             complement(6258..6944)
                     /locus_tag="ACHAUN_000526"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8459256.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(6937..7971)
                     /locus_tag="ACHAUN_000527"
     CDS             complement(6937..7971)
                     /locus_tag="ACHAUN_000527"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8459257.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(8034..8290)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8366..8704)
                     /locus_tag="ACHAUN_000528"
     CDS             complement(8366..8704)
                     /locus_tag="ACHAUN_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8459258.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(8706..9062)
                     /locus_tag="ACHAUN_000529"
     CDS             complement(8706..9062)
                     /locus_tag="ACHAUN_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8459259.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(9068..10255)
                     /locus_tag="ACHAUN_000530"
     CDS             complement(9068..10255)
                     /locus_tag="ACHAUN_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8459260.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(10446..11291)
                     /locus_tag="ACHAUN_000531"
     CDS             complement(10446..11291)
                     /locus_tag="ACHAUN_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8459261.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            11525..12490
                     /locus_tag="ACHAUN_000532"
     CDS             11525..12490
                     /locus_tag="ACHAUN_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8459262.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            12867..13910
                     /locus_tag="ACHAUN_000533"
     CDS             12867..13910
                     /locus_tag="ACHAUN_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8459263.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(13972..14436)
                     /locus_tag="ACHAUN_000534"
     CDS             complement(13972..14436)
                     /locus_tag="ACHAUN_000534"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8459264.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            14682..15629
                     /locus_tag="ACHAUN_000535"
     CDS             14682..15629
                     /locus_tag="ACHAUN_000535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8459265.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(15840..17912)
                     /locus_tag="ACHAUN_000536"
     CDS             complement(15840..17912)
                     /locus_tag="ACHAUN_000536"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8459266.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17845..18222)
                     /locus_tag="ACHAUN_000537"
     CDS             complement(17845..18222)
                     /locus_tag="ACHAUN_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8459267.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(18351..18911)
                     /locus_tag="ACHAUN_000538"
     CDS             complement(18351..18911)
                     /locus_tag="ACHAUN_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8459268.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            19061..20353
                     /locus_tag="ACHAUN_000539"
     CDS             19061..20353
                     /locus_tag="ACHAUN_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8459269.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(20413..21531)
                     /locus_tag="ACHAUN_000540"
     CDS             complement(20413..21531)
                     /locus_tag="ACHAUN_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8459270.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="ACHAUN_000541"
     CDS             complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="ACHAUN_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8459271.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="ACHAUN_000542"
     CDS             complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="ACHAUN_000542"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8459272.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            24737..25336
                     /locus_tag="ACHAUN_000543"
     CDS             24737..25336
                     /locus_tag="ACHAUN_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8459273.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="ACHAUN_000544"
     CDS             complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="ACHAUN_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8459274.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(27031..27375)
                     /locus_tag="ACHAUN_000545"
     CDS             complement(27031..27375)
                     /locus_tag="ACHAUN_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8459275.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(27512..28657)
                     /locus_tag="ACHAUN_000546"
     CDS             complement(27512..28657)
                     /locus_tag="ACHAUN_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8459276.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(28837..29811)
                     /locus_tag="ACHAUN_000547"
     CDS             complement(28837..29811)
                     /locus_tag="ACHAUN_000547"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8459277.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(29891..30766)
                     /locus_tag="ACHAUN_000548"
     CDS             complement(29891..30766)
                     /locus_tag="ACHAUN_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8459278.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            30896..32803
                     /locus_tag="ACHAUN_000549"
     CDS             30896..32803
                     /locus_tag="ACHAUN_000549"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8459279.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(33344..36622)
                     /locus_tag="ACHAUN_000550"
     CDS             complement(33344..36622)
                     /locus_tag="ACHAUN_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8459280.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(36893..37438)
                     /locus_tag="ACHAUN_000551"
     CDS             complement(36893..37438)
                     /locus_tag="ACHAUN_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8459281.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(37450..38142)
                     /locus_tag="ACHAUN_000552"
     CDS             complement(37450..38142)
                     /locus_tag="ACHAUN_000552"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8459282.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(38161..38445)
                     /gene="macP"
                     /locus_tag="ACHAUN_000553"
     CDS             complement(38161..38445)
                     /gene="macP"
                     /locus_tag="ACHAUN_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8459283.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(38458..39021)
                     /locus_tag="ACHAUN_000554"
     CDS             complement(38458..39021)
                     /locus_tag="ACHAUN_000554"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8459284.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            39249..39911
                     /locus_tag="ACHAUN_000555"
     CDS             39249..39911
                     /locus_tag="ACHAUN_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8459285.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            40035..41231
                     /locus_tag="ACHAUN_000556"
     CDS             40035..41231
                     /locus_tag="ACHAUN_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8459286.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(41332..42708)
                     /locus_tag="ACHAUN_000557"
     CDS             complement(41332..42708)
                     /locus_tag="ACHAUN_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8459287.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(42745..43611)
                     /locus_tag="ACHAUN_000558"
     CDS             complement(42745..43611)
                     /locus_tag="ACHAUN_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8459288.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(43700..44725)
                     /locus_tag="ACHAUN_000559"
     CDS             complement(43700..44725)
                     /locus_tag="ACHAUN_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8459289.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(44743..45417)
                     /locus_tag="ACHAUN_000560"
     CDS             complement(44743..45417)
                     /locus_tag="ACHAUN_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8459290.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(45601..46494)
                     /locus_tag="ACHAUN_000561"
     CDS             complement(45601..46494)
                     /locus_tag="ACHAUN_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8459291.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(46568..48265)
                     /locus_tag="ACHAUN_000562"
     CDS             complement(46568..48265)
                     /locus_tag="ACHAUN_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8459292.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(48258..49571)
                     /locus_tag="ACHAUN_000563"
     CDS             complement(48258..49571)
                     /locus_tag="ACHAUN_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8459293.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(49571..53512)
                     /locus_tag="ACHAUN_000564"
     CDS             complement(49571..53512)
                     /locus_tag="ACHAUN_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8459294.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(53621..54079)
                     /locus_tag="ACHAUN_000565"
     CDS             complement(53621..54079)
                     /locus_tag="ACHAUN_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8459295.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT        14984 a        11771 c         9813 g        17618 t
ORIGIN      
        1 tatatcaagg gttctagctc ctataacagg tttacccaac actgccttcc atctcataac
       61 tgatcaatcg attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa
      121 caaagcccat gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat
      181 agtagagttg ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc
      241 tgttgtggat ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg
      301 tgtcacattc gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc
      361 gatagtttac ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat
      421 ttggtgcatt gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg
      481 cgatggagag catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca
      541 tcgttgtttt tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt
      601 ttgctcgttt ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac
      661 ggcaatacgc atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg
      721 aataagtagg tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga
      781 tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg
      841 gaacatcgac tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg
      901 aattcagcgc tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga
      961 ataattcagg gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt
     1021 cgaattcatc tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg
     1081 ctagataagc tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt
     1141 ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg
     1201 catcgaaatc gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg
     1261 tttctagaga tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca
     1321 tctcacggat cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat
     1381 cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc
     1441 ttgtggtaat gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc
     1501 caggcaagtg tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca
     1561 gcatcgccta attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct
     1621 acagttaaat gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag
     1681 gcacttgccg tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg
     1741 tcttttacat cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc
     1801 ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc
     1861 acttgacggt ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc
     1921 catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc
     1981 gcgagcagtc gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc
     2041 tctgacggct accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc
     2101 caaattgaat gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa
     2161 tccatcaatt gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg
     2221 gatattgtcc ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc
     2281 ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta
     2341 taattcgaca aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca
     2401 ttttcgttta ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa
     2461 agcaaagaaa tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc
     2521 accatctact acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg
     2581 attgaagaca cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt
     2641 aatctttttc gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt
     2701 caagacagca cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc
     2761 catgtaagag agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc
     2821 tacccaattc aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc
     2881 atggatgaac gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc
     2941 taacgtatgg acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc
     3001 ctggagtact tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc
     3061 atcattgacg tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat
     3121 ccattcaact tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt
     3181 gctgttaaga cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc
     3241 aatcctctac tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca
     3301 tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct
     3361 ttatcagaaa gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc
     3421 aagatgtggc cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc
     3481 aaaatatgcc cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt
     3541 tgtctgcccg agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa
     3601 tctgagtcaa agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca
     3661 gaagcatctc gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat
     3721 tgatctacgg ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata
     3781 ttacctgatg attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat
     3841 tcactatgtt catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca
     3901 tcttgaatga ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg
     3961 aaaataccgc tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt
     4021 actgcttttt tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac
     4081 aagtcggtaa aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc
     4141 catgaacctt gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg
     4201 cctgtttcac tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc
     4261 acccgatcga tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag
     4321 gttctcatcc aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa
     4381 tcgagccagt ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg
     4441 attcctaatt ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca
     4501 gcattccctg tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata
     4561 tagttcaata atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt
     4621 tcatttactc ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg
     4681 tctaaaattg caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt
     4741 tctccgccag aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt
     4801 aaagccattt tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca
     4861 tcacgtttcg cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca
     4921 cttggatatt gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct
     4981 aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc
     5041 atgatagcgg cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt
     5101 tctccggttt tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca
     5161 tgtagatttt ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc
     5221 actcatttta gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc
     5281 ttttatttat tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga
     5341 aataagccat tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc
     5401 ctttactggt ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg
     5461 gtgaagcact tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc
     5521 aataatattc acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt
     5581 caaccctgcg ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg
     5641 gtacgtagaa gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt
     5701 ttcttcaata tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa
     5761 gccagcatct tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat
     5821 gattgttgca ccatctttta attcggaaac atctttgact tttttagagt acaagcccat
     5881 tggttcgatg tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt
     5941 gttcaaataa ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg
     6001 gttcggtaat acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa
     6061 taatggtttc acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa
     6121 tgtcgtgtcg tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc
     6181 taatcctaca gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta
     6241 catcccccta tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct
     6301 tgaacgataa atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta
     6361 ccacgctggt agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg
     6421 gccatcgcag taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg
     6481 ggaatacttt caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca
     6541 tctgctgctt ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca
     6601 taaaatggtg ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg
     6661 cctacgattg ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac
     6721 cggaaaatat tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt
     6781 ttgcgtccaa tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc
     6841 attgagatta atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg
     6901 atttgtttaa aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat
     6961 tcctactcct tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt
     7021 catcaagaca gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac
     7081 aacgttgaca tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt
     7141 tctttcgcta aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc
     7201 taatagttct gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac
     7261 ttcttttttc ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa
     7321 ctccatcaca gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa
     7381 gacgatggtc aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt
     7441 tgtttgcggg tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc
     7501 caatgctcgt gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc
     7561 ttgtccgcgt ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt
     7621 gctctttggt acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga
     7681 ccataacagg ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc
     7741 tttgtttttt aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag
     7801 ctcaagtcca ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat
     7861 tccataaata tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc
     7921 gtttttccca ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac
     7981 atctttctct tataattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg
     8041 acgaaagctt gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct
     8101 cttccagtac ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg
     8161 tagtaagttt gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt
     8221 tacccatttt cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca
     8281 aagctttctc gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca
     8341 acttttttca agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt
     8401 tgaaacttaa gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac
     8461 cgattttatc attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg
     8521 ctttttccgc ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg
     8581 gcagattggc aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac
     8641 agtattcttt tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg
     8701 ccattttata tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg
     8761 tcctctcgta agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc
     8821 gcttttgaag ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc
     8881 ccgcttgtgt tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc
     8941 gttgaggcct tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg
     9001 tctaaccacg ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac
     9061 aattttctta cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata
     9121 tcaacccgat accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca
     9181 atgggatccc tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga
     9241 acagcagcat catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga
     9301 aacacacccg aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac
     9361 cgataaagcc gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca
     9421 catagatatt gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa
     9481 gtccttgggc cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat
     9541 caatacgtgc aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt
     9601 cagtcgtaac caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg
     9661 cgatgatgcg ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga
     9721 caagcatcgt tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga
     9781 tcaagagctt cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat
     9841 tatgtttagt tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg
     9901 gctggaaagt aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat
     9961 tctgatcata gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag
    10021 gagtcagttt ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc
    10081 caactaaata ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat
    10141 catggctaag tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta
    10201 aaatcacgcc ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc
    10261 atcccttttc ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa
    10321 gtctttccat agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat
    10381 gcagtacaaa agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca
    10441 attatctacc agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga
    10501 aggatctctt cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa
    10561 taaaaatatc gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta
    10621 gacaaaatcg agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc
    10681 acgatcatct tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa
    10741 acaccagagt ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact
    10801 agctggtctt ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata
    10861 aaaccaaaat cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt
    10921 tcgatctcaa aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa
    10981 agatagacag caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca
    11041 ttgagcaatt tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat
    11101 aataagtcgg cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg
    11161 atattcaatt cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac
    11221 agtacttctg ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat
    11281 aacttaaaca tggcaccact tccataactt tttatgataa ttactataaa aactatttga
    11341 tttaaatata caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa
    11401 ctagtaactt agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc
    11461 attttacaat gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt
    11521 atatatgaag aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt
    11581 agcttccctt ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg
    11641 cggtatctct atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca
    11701 cacgaaacaa acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc
    11761 actcatggtt atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt
    11821 catttggctg tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca
    11881 tgttatcaaa gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt
    11941 tggatttggg gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt
    12001 cttttggatt tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt
    12061 tttcgtaaga aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg
    12121 ttctctttgc ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt
    12181 agcattgttc gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt
    12241 gttcaagctt cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc
    12301 aacagctata tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt
    12361 ggatttctta aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc
    12421 tcattacgta cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa
    12481 aatcgcttaa taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga
    12541 acggccaaac aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg
    12601 caagcttttg aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata
    12661 agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag
    12721 aagctacttc tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga
    12781 cctcttgttc ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt
    12841 atttattgac gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa
    12901 aggattcagc ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct
    12961 tcagcaggct tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat
    13021 tgggtatttt attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat
    13081 ttgctgaaag caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc
    13141 aaacgattgc tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca
    13201 ctatcatcgg tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga
    13261 tgatggctgg aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca
    13321 tccatgcgaa agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg
    13381 tgatgatgtt gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa
    13441 aaagtgcact tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct
    13501 tacttgacaa ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc
    13561 tcgttgttgt tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg
    13621 aaacgccaac tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt
    13681 ttgtgatctt ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg
    13741 cacattttat cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag
    13801 actttgcaaa attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc
    13861 ttctccaaac agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg
    13921 aaaaaacagt tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg
    13981 cagatatcct tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc
    14041 catatatcct gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg
    14101 caatggattg gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc
    14161 aacggctact tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca
    14221 gacattttgc tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct
    14281 ttttcccgca aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc
    14341 tgattcgctt tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc
    14401 taaccataaa aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac
    14461 tggaacttcc tcctttttct ctatccaaaa ctacttgcca actaataaaa attgttctta
    14521 aaagtttgat aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac
    14581 ctgttctttt acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa
    14641 tgtaaaccat atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg
    14701 tcccatgttt taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca
    14761 aaggtgccca tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag
    14821 cagcaatcaa gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa
    14881 attttgggaa agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga
    14941 ccgtgatgga tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga
    15001 tcactacttc tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg
    15061 cgatacgcag tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag
    15121 aaatggtcga tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc
    15181 tagaattaac agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa
    15241 cagaatatat ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct
    15301 ggaaattatt caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga
    15361 ttgcttcatt tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta
    15421 ttttgcgggc attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct
    15481 tcctgttcat tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga
    15541 aaatcttctt agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga
    15601 agccaaatga attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa
    15661 atgaaaaagg actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc
    15721 cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg
    15781 taaattcctt cttatttctt gaagctgacc acttctatca caacctcttt catatcttat
    15841 tacttcttca ttgctgcttt tagtgctgct gctaatgggc tttcttctgg ttcttctgct
    15901 tgtgcatatt ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt cttctgctct
    15961 tttttatcaa gcaatttttc tgtcgttcca tcgtatttac aacggaaata agcaccgttt
    16021 ttaccttcga tgatttccat tttccggtga cattgcgggc atcgatgatt agagactttt
    16081 ggatcttttc ttctgcggta gttgcaatct gaattggtgc agacatagat tttgccgtca
    16141 cgtgtgtttt tctcgcgcag attggcccca cattctgggc atttcttttg ggtaatcgag
    16201 aaatcttgat attcttgttt gcttgatttg atctcattca ccaagcgttt tgtttctttt
    16261 tcgattccat tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct
    16321 ttttcccagc tttctgtcaa atctggtgtt actaaagagg gatttaccaa tactaataat
    16381 tgcttacctt taggagatac ttgcagtcct tgaggggtac gttccatcag ttcagatttg
    16441 attagtttct cgataatctc tgcacgcgta gctggggttc ccagactgta tttttccatt
    16501 tgaccaagca agcttgcttc tgacaatggt ttaggcggtg cagatagttc ttttttgatg
    16561 gcgaaatcgg gattgattct cattcctttt tcccatttga ctgtttccgt ctcagtttca
    16621 gtttcgcctt tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta
    16681 ccaaattcaa ccgtaacttt tgtttggctg actttatggg gttgagcaaa cagtcctaaa
    16741 aaacgagaga caatcatttg atagattttt cgctcttcat tacttaattt ttcataacgt
    16801 gctctgtttt cagttggcaa aagtgcatgg tgatcggtca ctttactgtc ttggaatact
    16861 tttgtctgtt ggacgatggc tccgtttttc aaatactgct tgacctcagg agcaaagtcg
    16921 ctgactgctt gcaatcgttc tttcatcgta cttttcatgt catttgtcag atatttgctg
    16981 tctgtacgcg gataagtaac gatcttatgg gtctcataca aactttgtac aagacttaat
    17041 gttttctttg cagaatagcc atagcgttga ttggcttctc tttggatctc tgttaaatca
    17101 taaggcaatg gcgcagattc cgtttttaat ttttcttgga cttctgtgac agttcctttg
    17161 cttgtactga tcaattgaac aagtttttgt gcttcttctt gtgtttttaa agcaaattga
    17221 tttttttgaa gcattttggc ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt
    17281 tgtggacgga actgctcgat tttcttttct tggtctctca ctaatgctag tgtcggtgtc
    17341 tgcacacggc ctgctgataa attatccgcg tacttcacag ttaaggctct ggtcacattc
    17401 aatcctacca gccagtccgc ttttgcccgg gcaagagcag attcatacaa tgcatcatat
    17461 tctttcgcag gtcttagttg cttgaatcca gttttgattg ctttatctgt ttgagaagag
    17521 atccataacc gcttcactgg tttattgaaa tgcacatact ctaagatcca gcgagcaaca
    17581 agttcccctt ctcttccggc atctgtcgca atgactgctt ctgagacatc ttttcgctga
    17641 gccagctgct tgattgcttt taattgatgt cctgttttag gaagtggttt gattcctagt
    17701 tttttaggga tcattggcaa cgtgtccatt tgccagctct gccattcctt attcagatct
    17761 tccggcattt tcaatcctaa taaatgacca agggcccaag taacgatcac cttgggtcct
    17821 tcataataat ttttgtgctt ttgattagca ccaagcacct tgcttaggtc tttcgcaacg
    17881 cttggttttt ccgctagaat tagttgtttc ataaaattcc tttctgcttt ctactgtgat
    17941 gggctgatga tttttcaaca acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc
    18001 ttcacaccag cgctcatctc cgtcattcca aaaggcgctg agaaagttac tttttgcatt
    18061 gatatattca taattttgat ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc
    18121 ttcgaaagac tcgaatgttt cttcttcttc aatatcttct tcccagtctt caaaaaacca
    18181 ccaaggctca ttgtccccga acatggtaat tacttgatac atactcgtgc tccttctttc
    18241 agcttttcta gactcatctt agtcactttt caagaaagtt gcaagaattt tctcttataa
    18301 aagggtcatt taatgaacat cttaaaattg ttacaaatga atctgattgt ttaaggcaaa
    18361 gtagcgataa agttattgat catccaaaaa tagctgtctt ctggatcgat tgttggttct
    18421 tttgtaaagt atcctaaaaa acgtaaggaa agataaccgt ggatcatact acgaagttcc
    18481 ctgcttcgat gaactttttc ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg
    18541 atctgattcg tttcatggat tccatctaat aaaacagctt cattggtatg cggtacactg
    18601 atcagtaact catacactgc ttgatttgaa aaagcaaagg atcgataagt ttctgcatag
    18661 atctgcagtg cttcctgacc actttttccc acaagagccc gtcgcagctg gtcattaagt
    18721 tcctgcagaa gtttcgctgt tagcgctgtt ttgacatcac gaatattttt gaaatggtta
    18781 tacagcgagg gagccttgat atccaagcgt ttggctaaat gctgaaaggt tatttgctga
    18841 acatccatct cttctgctag ttctcgaaag cagtcgatga ttttctcttg agacagattt
    18901 ctttttttca tttccatgtt tcctctcata aaataaatcc ttcactcttt cagtttacca
    18961 cgaaaaagaa agagaaaaaa gagactttca ttgataaact acatattgta gtttataata
    19021 aagagctgta gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa gaataaagga
    19081 aaaattattt aggaaaagag aaacttggga acaaaacttg tttgtcttat ggtttggaac
    19141 atttatggct ggtatcggat ttagtttagt catgcctttt atgtcacttt acatagatac
    19201 gttaggtaat tatagtacgt cacaattgaa cttttggagc ggattgactt tttcatctac
    19261 tttcttggtg actacgctta tctctccttg gtggggaaaa ttagccgacc aaaaaggaag
    19321 aaaattgatg ctgttgcgtg cttcattagg aatggcaatc gtcatttctt tgatgggttg
    19381 cgtgactagt gtgtatcaat tagtcgcatt gcgtctgctt caagggattt tttctggtta
    19441 tatcagtaat gccactgccc ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt
    19501 tcttggaacc ttagcaactg gttctgttac aggaacatta cttggaccgc ttcttggcgg
    19561 gatctctgct tcagcgtttg gctatcgccc tacctttttc attacaggga tcatcttatt
    19621 tttcgtcttc ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga
    19681 aaacatggtt tctgccaaac agattttcaa agacttgaaa tatccacacg tcgtcatcgg
    19741 tatgttcgtc acaacgatga tcatccaagc atccaacaat tccattagtc caatcatcag
    19801 tctgtacatt cgccaattgc tgcatggaca tgggaatgtt acgttgatca gcggaatcat
    19861 tgcttcgata cctgggattg ccactttgat agccgctcca agatttggtc gcttaggtga
    19921 caagattggg agtgaaagaa tacttgcgat tggtttgggt tttgcgattc tggtttatat
    19981 cccaatggca ttcgtcacga atgtttggca attagctgcc ttacgttttt tgattggtat
    20041 ctctgatgct tgtctgctcc ctgctgtcca agcattgatc acaaaatatt cgcctcatca
    20101 agctgctgga cggatcttca gttataacca atctttccaa gcaactggta acgtagtagg
    20161 gcctatgatc ggctccagtg tttccagtgc atttggttat cgcggcgtct ttctttcgac
    20221 ttctcttctt gttttggtca acttcttgct tgtacgacac aatacacaag agatccatca
    20281 gcaagaaact gcagaagaaa aagcttcagg agtctccata gcttcagggc actctccttc
    20341 tcatagacaa taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag actgtccttg
    20401 cagtgttttt gtttaggcct gttttttcaa gaactcagat aagaggaata atcccttttc
    20461 caaggtttct tttttacaac agtaacctag tcgggcatgc ttcggtaaat caaaggcact
    20521 tcctggtact aatagtactc ctgtttcttc taataaattg atacaaaatg tctggtcatc
    20581 ttcttcgatg gctaatttaa tgaaagaagt agagacgtaa ttgggagcga caagttcaac
    20641 tttcggttca ttggctaccc attgttttaa aatcacaagg ttctctgtaa tgatcttttt
    20701 gtttcgcgcc aatatttttt ctttattctt caatgcatgt acagcaagat catctgacag
    20761 gactccgccg caaatcatcg tgtagtctcg atactttcga aaaacttctg ccaactcagg
    20821 tccagtagct gtccagccaa tacgaatacc tggaatggag taggttttgg atagggaatt
    20881 ggttgctata cctttttcat agacatctac gatcgacaag aattcacctt tatccgttaa
    20941 aggcgcatat acttcatcta tcaatacata tgcatctact gtacgagcaa tttctgcgat
    21001 tttttctaaa gttttccgat ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca
    21061 gatgattttt gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa actgccaatc
    21121 ttcttcttcc ttcaaatgaa caaagtcaac agcggcacct aaagatttgg ggatgtcata
    21181 taattgctga taagtaggaa gcatcgaaac aacatggtct cctgcttcca ctaacgcgta
    21241 tagagctaaa agattagcac cagtcgctcc attcgtttgt aagatgtttt ccggatcgac
    21301 ggtttgataa agagaagcga cttcttgttt gaaaacatct gatccttcga tccagccata
    21361 gtccaatggt tcgtttttgc gtttttcgaa aaatttactt acgctagtcc catctaagcc
    21421 aacgagttcg tctaatgtca atgcttcgat cgagctttgt gcaatatcat ataccgcttt
    21481 cttttcccat ttatttagcc attcttctac accgaaattt gcgattttca tgtttctttc
    21541 ccccttatat cttgatccag cctacagcaa gcaacccgat tgaaagcagt gcggccacac
    21601 aaatcagccc aatcattact ttttcttttc ccgtaatttg atgtttggtt tcacgacaag
    21661 cgatccagta gaagcaaaaa ccaggaagat aggcaatact acataaaagg acataagaaa
    21721 atcctgctaa aatgatgccc atcaattcaa aaactacagc aatcccaccg atcactactt
    21781 gttttttgat tccttctttt aaagaaaact ggacttgata gatcccgaca agtagataac
    21841 aaatcaaaat tgccgctgta cataaagaat aggcaaattg ataggcataa tcagtaacta
    21901 agaaagtcaa taagaataga ttagtcaaga ttgcagtaac aactaacgaa taagttgggg
    21961 catttttttt attcgttttt ccccaacctt tcggcaacag accgtctcga gccatcaaca
    22021 tcgttgtttc tgctgggagc atcgtccaag acaaccaaga accaatgatc gaaataatca
    22081 agccgatatt gatcagcgct gctccccaat ttcccacgat tccttttaaa atatttgcca
    22141 tagcaggttg tgaaatagtt gctaactctt cttgagataa gacgccgtat ggcaagatcg
    22201 aagcaaggat atagataaac aataaacaaa gcagaccaat gatcgatgct tgctgggctt
    22261 ctgaacgttt cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc
    22321 acatcataac catcaaacaa cctttaatct gcaaccagat cgttccttgc tgccctgttc
    22381 cattcactag attatcagat acgttacccc aaaaatcagc agtgaatata ttcactttaa
    22441 atgcgataaa tacaaatatt aaaaatagga ataatggcac taatttacag atcgtcacga
    22501 ttgtattgat gacgctcgct ccttcaactc cattgtttac taataaagtc agaccccaaa
    22561 taaaaatact tgcaaataaa atagaaggaa cattttgtcc acctttgaat atcgggaaaa
    22621 attctcccat ggcgctcatc agcatcgttg caaaagcaac atttcccagc cacgcagaca
    22681 accagtaacc ccaaccactg atgaagccac ctaatttacc aaatgaagct tccgcgtaac
    22741 cgaaaatacc accatctaga tctggacgct tttctcctaa atgattcagc gataatacca
    22801 aagctaaaat accaatccca acgattatcc atgaaaggat cgctggccct ggagctgcag
    22861 aactagccag atcacttgtg atcccaaaca caccgcttcc gattgacgaa ctgacaacca
    22921 gagcaaccag tccccatttg ctaatacccg tcgatttctt ttccatttcc atcaccctca
    22981 cttataaaat tgaacaaatc acttgcaaaa gattcccttc tattttgcaa ctgctcttgc
    23041 ttaacaaaaa gcaactttat tcatattatt ggatattatg aactttttac acaagtattt
    23101 caagtataaa atgaataaaa attaatttta ttgcaaaaaa acagctatgc tatccaaaag
    23161 aaaaggtgtt tcttttgtta gccatagctg ttttgattca tttaatatcg cttaaacgta
    23221 ttgtaaaact tttgtttctt gatatgcgtg gtcgatcaaa ccaccaccca gacattccat
    23281 tccatcataa aatacaactg cttgtccagg agtgattgca cgtacaggtt cgtcgaatac
    23341 gacttctgca cgattatcat ccaataagcg aactgtcaca ggaacgtctt gctgacgata
    23401 acggaatttc gctgtacact tgaattcttt tggcattggt tcattcgtcg taaagtggat
    23461 ttcacttgca tccaagcttg tggcatacaa tgcaggatga tggaaccctt gccccacata
    23521 taatgtgttt gtcgctagat cttttcctac aacaaaccag ggttcttgcg aatctcctcc
    23581 gccaccgatc cctagtcctt gacgttgacc aatcgtataa tacatcaagc cagcatgttg
    23641 tcctttgact tcaccatcaa gagtgaccat atttcctttt tttgctggca gatagttgct
    23701 caaaaattgt ttgaagttct tttcaccaat aaagcagatt cccgttgaat cttttttctt
    23761 agcagtcgca aggcctgcgc gttcagcaat tgcccgtact tctgattttt ccatgccgcc
    23821 tagagggaac atcgtttttg aaagctgttc ttgtgataat tggctaagga agtaagtttg
    23881 atctttatta ttgtctactc cacgaagcat gtggaccacg ccgttttcat cgcgagtgac
    23941 ttgcgcataa tgtccagtag ctacataatc ggcgccaaga tccattgcat aatccaagaa
    24001 tgctttgaat ttgatttctt tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg
    24061 gtattcggct aggaaatatt caaatacacg atcccaatat tctttttcaa aattcacaga
    24121 gtaataagga atcccgattt gagcagctac tttcgctaca tccttataat cttccgtggc
    24181 cgtgcataca ccgttttcgt ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc
    24241 gtatccttgt tcctttaaca aaagtgctgt tacagatgag tcgacaccgc cgctcatccc
    24301 tacgacaacg cgtgttttgc tgttgtctgt catgatatca ccatcatttc aattagattc
    24361 tgaaacatct acgagttgaa ggtcgcaact tttcagtatt ttacattata cgtcatattt
    24421 ctgaaaattt catcccttaa ttatcaagtc gtaaaaatac tgttctactg gacctattca
    24481 gtacacaaac atgatagtca accaaaaaat cactcatctt ttgagcattc gatataaaaa
    24541 attggcatac tcataaaaag ggccagcctt tcggcaataa ggaataattt tataaattgt
    24601 gaaaaactat ttttcaaaat tgcttcttat cgcttaaagc tgcacgtctc atttacaacc
    24661 ttttcttttt agatgttatg agttgctcag ataagtgatt ttgtttactc taagaaaaaa
    24721 gggaggcaaa agatacatga attactttat cagtgatatg cactttttcc atgaacgctt
    24781 attaggaaaa aacgactttg ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca
    24841 gttgatcact agctggaatg cagtaataaa agaaaccgac catgtttatc atctaggcga
    24901 ccttgctatg catccgcaat atgaaaaagc aaatgcagaa attttggaac tggtcactca
    24961 actgcaagga acaatccact tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta
    25021 tttagaaaaa aatgatccta ggcttactga cggaagtccc aaattttatt tttatgatgt
    25081 aggtgccatc gttaaattcg accatcaaca atattacttg actcattatc cgatgttgct
    25141 cgggcctaac gacactatcc gcaatcttca tggacatatc catcattaca gcgtacctat
    25201 cgagaacgat gtcaatgtag gcgtagacgc accggaaaga gaactcttaa aggaaaaact
    25261 accatttggc actccgttat ctgagcaaca aatcaaagaa atctatgaag caaagaggaa
    25321 gtacgatagg tcgtgaaaaa actgccctat atcaagtatt aaacaaataa agataattac
    25381 tcatattttt catttgagta ggcttattgc cacaggaaca ttaggatcag aaatggagtt
    25441 gtaacaagac ttctgtcaca actccattcc atactattcg tctttttgct catggtgttc
    25501 ttttaccagt tttttctgta attctttatc ataatttcgg gcagctgacg ttaaaaataa
    25561 aacgattccg cataataaaa tcccgattcc ggaaaacaag aacatttttt ctacccctaa
    25621 acgatcagct aatggtcctg caaataataa tccaattgga cctgccaggt tcatcaaaga
    25681 gttgaagttc cctaagacac gacctaaaac attcgattca taactctgct ggatcattgc
    25741 catcagcagt gtattgaaat atggcgttgc acaacctgct cctgcattca agataagaaa
    25801 gtacaagaac ccttgactat cgtttggaac caatccgctt gcaccgatcg tcaggcccat
    25861 aatgatgtag gctataataa taggtttcat tcggtctttc catgttccta taaaactgat
    25921 cagtgcaccg cctaagagca ttccgactgc gtagactact tccactgctc ccgcctgtcc
    25981 aacggaacca ccaaagtaat ctagcgtcat caatggatac agactgattg ctggcatgaa
    26041 caacagcatc acaaatgcac cattgatcgt tatgtaccat aaccctttat tatccatcaa
    26101 ttgctgcagc ccaaattttg catctttaaa aatagtaagc ggaacttcca atatttcagg
    26161 agaaactttt gggatcttca caaaaatcaa taagccgaca ccaaaaactg cccccaatac
    26221 atctaataaa ataaggtaat tgacaggaac gactgaaaac aaagccgcac ctagagctgg
    26281 cgcaatgatg aaattcgctg attggaccat tcctaactgt ccattcgtcc ttgttatgtg
    26341 agaagatgga acgattgtcg gcataatcga ctggatcgtt ggcatttgga atgtttgtgc
    26401 gaccgaacgc ataaacaaag acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt
    26461 tccgactact gctaaaataa gcgcgaaaat cgctacaata atatccgtga caatcaaaag
    26521 agctttttta tcccagcgat ccactagcgg tcctacaaat gggcttagta aaaccatggg
    26581 aatcattcct agtaaagtag caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa
    26641 ataccagatg atcgcatatt ggacgaccat actagtgatt cctgacaaaa actgtcccga
    26701 taaaaagaac aaataatttc tcttccaatt cgttaaaacg accgactgtt cattttccat
    26761 aaacatccct cttttctttt tcattttgta tagcgtttgc acaacaatca ttatacggga
    26821 ttcttttttc aaaaaatacc tttttcccta aataacaatc gtttcaattt aaatttcttt
    26881 acttagaaac aataaatcag caaaatatta aaaataacaa catgtgttaa atttaaaaat
    26941 aaaaaagaca actaatttgc gtcgtcctta caaagttagt catctaacaa taggccgttt
    27001 caaaactagt tgtcttttta tatccacaga tcattcgact ttttcgaata acccacggct
    27061 aatcatccca tccatcagaa aataaaattt atcttggctc attttctgtt ttggatcttt
    27121 gaaaatattg actgctttca atccatttgc tgtagtgatc gacattttca atgtttgaat
    27181 atcttttgat acagtgattt ttaatttgaa tccttctttg ctttgcggcg tggcatctaa
    27241 tggtcggatc aattgataat tgcctccgtt tgtttctgta aatccattat cgcgcaatgt
    27301 atatcttttt gcatcaggat gcaaacgata gctaattggt gcgcccagta ccgttgctgt
    27361 tgtttcaaat gccatgattt tcacctcgat aaaattagtt tctctttatt ataaaagttt
    27421 atcctgattt ttactaggtt tttcagttaa gttccctaaa aaatgaaggc gttctctcag
    27481 acatctgttc atataagaat gacttcacta gttatttttt gacagcacga actaattgat
    27541 caaggaaata ctggatgtcg tcttcggtat ttcccaaacc aaagctgata cggatggatt
    27601 cttttagtat cggtgtatct tctccataca ttgcttctaa tacgtgtgaa ggctcgatat
    27661 ttcctgctgt acaagctgaa ccagtagaaa tagcgacccc attaagatca agcttcatca
    27721 gtagcaaatc atttgcttcg ccaattaagc gaagattcaa cacatgaggg agtttattcg
    27781 ttaaatcacc atttacttga taagggattt cgtttgcttc taatgtatca agaatcaatt
    27841 gctggaatcc tttatatttg ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa
    27901 ctgcttttgc cataccccag atccctgcta aattttctgt acctgcacgc cgcttttctt
    27961 cctgctcccc gcctaaaagc aatgcaggga tatttactcc gtctcgttta tataagaaac
    28021 cgacgccttt tggcccattg attttatgag cagagatact taataaatcg attcctaatt
    28081 cttctggatg gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg aaataagctg
    28141 gatgatccgc taaaagttca ccgatttctt tgattggcat caaatttccg atttcattgt
    28201 tcccgtacat cacagagacc aagatcgtat cttctcgtaa cgcttttttg acctcttcga
    28261 tcgacagatt ccctttttca tctacaggca gatatgtaac gtcaaacccc tttgtctcta
    28321 aatatttcat ggtattaaga acagcaggat gttcgatagc tgtagtgatt agatgtctgc
    28381 cttctttttg acgcgagaac gctgtttcta agatagctgt attatcccct tctgttcccc
    28441 cactgttaaa gatgatctcg tgaggttttg cttgcagact gtctgcaatg atttgtcttg
    28501 ctgtttccaa tttttcatgc gcgtggcgac caaatccatg tatgcttgaa ggatttccaa
    28561 acgtgtcttg catgattgcc gtcatcgctt caattacttg tggatgcatg ggggtagttg
    28621 ccgcatgatc cagataaata gttttcactt cattcatccc ttccaaatta ttgctaggca
    28681 cattctagca taaaatagcg aacaaaccaa agaagagctc atttttgtct agaggacgtt
    28741 tcatgggcat agaaaaaagg agctgtgaca aaagtcttgt cgcagctcct tgaagctgct
    28801 cacttctgtc acaacttctt tccctctttt ttttgtttat tcgaattctt tttcttccag
    28861 acggaataac ggactcatcg gtgtattttc atggatgcgt ttgaccgctt cacccattaa
    28921 ttctgaacat gtaacgattg ttaagttttc tggatgacga ttttcttctg tgaaaacaga
    28981 atcagtgata caaatatctt tgattggtgc attgtctaaa atacttttcg catgatcgga
    29041 caataatcca tgtgacgcac aggcatatac ttcttttgcg ccgttttctt ttaatacatt
    29101 tgctgctcgt gcaagcgttg ctcctgtgtt caagatatca tctaccatga tacatgtctt
    29161 tccttgaaca tcaccgatca cataaccacc ctcagttgaa gcatcatctg catgatctac
    29221 gatggctaat gtgctgttca aatattctga aagactacgt gcacgctgaa caccgctgtt
    29281 ttttggcgaa acaactacga tgtcatctcc cgtcagacct aattgacggt aataatgagc
    29341 aaacaacggc atcgtaaata aattatctac aggaatatca aagaatcctt gtacttgaac
    29401 cgtgtgcaga tctaaagtca gtactcgagt tgcccctgct tcttcgatca agttagcgat
    29461 caatttcgca gtgatcggtt catgaggttt cgccgtgcgg tcttgcctag catacccata
    29521 gtaaggcaat acgacattga tcgttttcgc acttgctcgc ttcattgcat cgatcatgat
    29581 cagtagttcc atatagtaat cattgacagg tgcattcgtt gattgaacga tatacacatg
    29641 atcccctcga atactttctt cgatattgat ggaaatctcg ccatcactaa attgcttcac
    29701 gacgctttta cctaattctg ttccgaacac tttcgcgatt ttttcagcta atggacggtt
    29761 gccattcaaa ctaaaaatct tcaacgtgtc atcctgatac ttattactca tgtagtcctc
    29821 cccaaaactt ctctattctt cgatagttta acatattttt ccaagatttt ctgttaattt
    29881 cagcaaattc ttaaggaagt gaaactgttc cgttactcca actggcagga atgactttat
    29941 gataatcgcc ccagttgtcg tgtactttgt aataacgttc tcctgtttca gtttcaaaat
    30001 aagcgtacgc tgtcacccaa tgattgccat atgtactgcc aagtactttc aaaataccaa
    30061 tcatcacagg tttgccttca cgtatacgtt ttgtagctcg ctgccatgaa cctagaagag
    30121 ttgctcgtgc acgctcatga ttgccattct tctttaaaaa actagaaatc cctgtactaa
    30181 cctgaaaagg aatcgtagga agtcctagtg gttggatctg gctacgcaat gccttcgtca
    30241 aaaccaacga atcagccgta tttttcttgc gaatctcatt tggaagcatc caccccttac
    30301 gatagtcttg ataataggca aggagaactg ctgccgcgta agtaccacaa agatagcctg
    30361 agggggtagc ccatttctta tacctcaaaa aacgttcttc tggcaatccc acccagcaat
    30421 ttgcgagttc ctttcctgac cttttttctt cgatcaaaac gggaatatct tcttttctgt
    30481 tgatccatct agataactcg attggctctt cattgatcag cttccatttt ttacaagaaa
    30541 gaccctcttg ataagtatat tttgctggag aatattctac cggatgtcct gattgatcga
    30601 taatcacata actttgatct tcaaaaacca gagctaaaaa aagcaattta ctttttcttg
    30661 actgcagctg ctgagtgctg gctaattttt taattttttt atctactttg aaatataaag
    30721 gtttatccac tacttgattc agttgggatc gcctcctttt tttcattata agtgaaggat
    30781 tgaaaaaaac aaatcagttt gattgttcga attttcaatt ttttatgttc tatttgttaa
    30841 ttccttcatt ttgtaagata atgggcacac gaaacaaaaa aggagcgatc gatgaatgaa
    30901 caaagaacga ttgaaacaag atttctatga agcagtcaat gaagaatggc aaaaaacagc
    30961 aaaaatccct gcagataaac cagctacagg cggtttccaa gatttagttg acgggatcga
    31021 ccaacttttg atgggagaaa cagacgatct gcttgcccat ccagaaaaag tgtcttcaga
    31081 aaaaatgaaa catttccttg catattatca actggcaaac gattatgacc gaagaaatga
    31141 gttgcatgct caaccgcttc aacctataat cgaaaaagtt gaatcattgc agtctttcga
    31201 cgaattgaat aagcaatttc cagaatggct gttggataca ttaccattgc catttaatct
    31261 ggatgtagat gcagatatga agaatgcgca aacgaacgcc ttctttgctt atccgccttc
    31321 tttattcctt ccagataaga cgtattatgc atcagaccat cccaacggtg cacaactaat
    31381 aggcgttttc tttgatatgg ctgttcagtt attagagctt gccggcaaag aaaaaagtca
    31441 ggcagaagca atcacggaac aagcagtgca atttgataaa ttgattgctc ctcatgtaaa
    31501 aagtgcagaa gaaaacgcag attacagcaa aatgtataat ccgcaatctt ttgatacatt
    31561 tattaaatac acagataaac tggacttgaa aaaacttgtc acgggtttga ttgggacaac
    31621 tccagataaa gtgatcgtaa ctgatccagt gtacttcgat catttgaatg acatcctgac
    31681 agaagctcat tttccgttat tcaaaagctg gatgatcatt aaaacagtac gttctcttag
    31741 tggttatcta tcagaagaat tccgccaagt cagcggaatc tattctcgcg tattgtctgg
    31801 aacagacgaa gcaatgcctc agaaaaaagc agcgtattac cttgcctctg gacaattcga
    31861 tcaagtagtt ggcgattact acggaaaaaa atattttggc gaagctgcta aaaaagacgt
    31921 agaacaaatg gtgaaaaaaa tgatcgccgt ttataaaaaa cgtctagaag aaaatgactg
    31981 gcttggggat gcaacaaaag aaaaagccgt cataaaatta aataacttag gtatccaagt
    32041 aggctacccc gataagatcc cagcattatt cgaccagttc aaaacagtac ctgcagatca
    32101 gggtggtact ttgctttcaa atactcttgt tttcagcaaa ctcacattaa aagatcgttt
    32161 cagcaaatgg aacaaaccag tggatcgttc agaatgggaa atgagtgctg acacggtcaa
    32221 tgcttattat catccatttc ggaatgtgat cgtctttccg gcagcgatcc ttcaagcacc
    32281 attttacagt ctagaacaat caagctctgc taattatggt ggaatcggag ctgttatcgc
    32341 tcatgagatt tcccatgcat ttgataataa cggcgcttta tttgacgaat atggcaactt
    32401 gaacaactgg tggacagaag aagatcttac acaatttcaa aacaaagcac aagcaatgat
    32461 cgaagaattc gatggtctgc cttttgctga cggaaaagta aacggaaaat taaccgtttc
    32521 ggaaaatatt gctgatgcag gcggtctaag ctgtgcattg gaagcagcaa aaacggaatc
    32581 agatctttct atcgaggaat tctttgtcaa ttgggcaacg atttggcgta caaaagcaaa
    32641 aaaagaatac caacaacttt tactgcaaat cgatgtccat gcccctgcta aactccgagc
    32701 aaacgtgcaa ccaaaaaacc ttgacgaatt ctatgaaaca tttggtatca cggaacaaga
    32761 cgctatgtat ctagcgccag aaaaaagagt gcgaatctgg taagaggttg tgccagaagt
    32821 ggtcagcttc aagaaataag aaggaatttg ggaaaatcgc ttctcaaatt tttgtgacat
    32881 ttcggcttat ttttcgaaga agttacttct ggaataccat gtattagagg ttgtgaaaaa
    32941 gctgcttagt ttcaagtagt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
    33001 aaatttgact tactaccgag aaactggctt ttgaacaccg tttatcgaga ggttgtgaaa
    33061 tcagcgtcct gacctgagta ttagagaaaa aaacggaaaa atagttcctc atattttgcc
    33121 gtttgttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gagattgtga
    33181 aaaagctgtc ctgcatcaag taataagaac agccaatcaa aagcagcaag gagaacctca
    33241 tctataaaaa gatagaagga agtgagacat ctattggtct catttccttc tatttttact
    33301 ttcctcagtc aattgtttta catcataaaa ggagaaattc cacttatttt tttcgctgtt
    33361 tcatgacata aacaactaca ccgattaaca aaattcctcc agctaatgct agcaacgggt
    33421 tattaaattc tcccgttttt gggagtcctt gagtgccatt tttttcactt tcttttgctg
    33481 tcctatccgg atgagaaggt tttccatttg gttttccatt tggtgattca tttcctggat
    33541 tttctctttc tggtttgtca tttcccgggg gcacatcggc agtttctcgc acagtgatcg
    33601 ctttttgctg ggtaacagat tctgtatgag cagaccattt ttccccatca aatatttccg
    33661 aacgataagt gacttctagc gtatattctc cttttgaagg tgctgaaaaa atagcggtat
    33721 gattagaaaa ttgagcactt gactgtgttt caacgatttg gatcttttct gggaaataac
    33781 gcatatcttc aaagacaggc gttccttgat ttggtgtgaa agttgacaca aaaactgtaa
    33841 aaggcttcag ttcaatgata tttccttctg cattctccgc cacaccattt gcttcttgta
    33901 taataatcgg attattttgt tcatttggtt ttgtctgatt tggttcttgt acgattgcat
    33961 actctggata ttccgaagac ggtgaggttt ctccgtcaaa aatagcacct acagataatg
    34021 gaagaccatc cactctttcc ttttctgttt gacgattccc cggtttaatc tgcatcagtc
    34081 catgaacatg gatacttcca ttttcatagc gcgtaacagg ctgctgacct tctttcgaac
    34141 atgtatttag agactcgatc caatcttttg atatggtatg tccaagagta ttttctgatt
    34201 gtttctctcg ttcattccaa tagtagttca acgagtggta gatttgatct tcgttttgat
    34261 tcaatgggcg gatctgctct ctcatctcaa gattctctgt tcggaaagat aagataccag
    34321 aagcatggta gtctgtgttt cctgctgaag aggtataaag agccacatta tatgaaccgt
    34381 tattaaaact tgtactgcta ttgacgataa tatctgttcc gctattggaa tcgattcctt
    34441 tcgcaagatt ttcgaaagac aaactattga tcaattcatg agggcccgtt aaagaagaac
    34501 cgcccatttt gaatccatta ccgttacctt ctccctcaat tcctggtacc cagccgtttc
    34561 gataagcaac tgagtttttg atgattactt ttccgatcga acctgtttca tttttagcaa
    34621 aaaggtccca accgtcatct gcattatgat acgcgataca accgtcaaat acatttcctt
    34681 ctcctattgt cagtttggcc gcaaatccat cggcatcttc aaatcctgga tcggcatttt
    34741 caaaagatgt acagttcttg actaagttat gcgcgggcca tgcttcgaaa ggatcatttg
    34801 agctaccgct gatttggatt cctgtatttc cattccgata agcatgaatc tcttcgatca
    34861 aattatgatt tccagaaatc tgtaatcctt tttgcatagc tgcactattt gtaatgtcaa
    34921 accctcgtaa accccagtag tgactgaaca gtgtcatccc atttccagtt cctttaaaat
    34981 caagtacagg acgcttgttt tctggatttt tctctgcaat caactgaatc gggttttctc
    35041 ttgtcccgtt aacgcctttt ggaatcgtca atcctctttc gaaggaataa gttccaggcg
    35101 ccatgacgat tgtctgccca gcatatgcgt atctcaatgc ttgtgtcaga gataatggat
    35161 tttgtctgct tccgtttcct gcatctgacc cttctggtgt tacataaact gtcgaatatt
    35221 taggtttacg gtagtccact gtatgaagaa tctcttttgt ttcataacta gtcatctcta
    35281 cgtattcttc tggcggatag tttttatctg gcgtaaaact gaaagtaaat tcgttcgttc
    35341 cttccatcag ttttgtctga taatcaaatt cccgattcgc agtgatagcc aagttttttg
    35401 cgttcttaag cacctctcct gttgctttat ttttaattgt caataatccg tcagcatttg
    35461 ctctaaagga catagtatga tcttggtttc ctgaatgatt actagaggtc acagtgtaaa
    35521 tcggatcaat caagcgagta ggtctttctt gcccaggagg atctgtcgca ggatctgagt
    35581 aagcaacttc gattccttct accttgatcg tcatattcct tgctgcagca aatcctgcat
    35641 aaataaccga ttcatctgaa gcgtataatt tttcccagtc atacatgatg tcagaagcca
    35701 tttcttctcc tgtttcagga tcgtaataat atgcctctaa accagtattc gtttttttca
    35761 tctctacgtc cagtttagta atcgagttcg tggccggaaa cagattccca tgaccgtttc
    35821 cgaatatatt ataagaacct gtttcttttt ccaagaaccc tgctgaagtc tctaaaggtg
    35881 ttgtttccac tgagacactc ccaggagctg gcgcagcttg cggatcttca gatgatattc
    35941 ctgtgattgc ccgtgtcccc aatccaagtc gcatcgtata tttgctgccg ctcgtgttga
    36001 ccacttcttg tctatccgag tcccagacat attcgatccg actgcctagt atggcaaatg
    36061 aatttgaata aaatgcctgc ccaaacaaat gattttttgg tactgcgtct cttaccatta
    36121 aagcaaatcc ttcttgggca ttcgtatagg tccaagattc tatttcgatc gtcgcccgca
    36181 tcctaaagtt gatgtctttg gggattgctt gataataata ggtcattcca tctgatccgc
    36241 ttgactggaa ttttccgccg ttattcgtgg aagagagccg cacgccgtct tctaatggtt
    36301 caatggcata cctactagca cttgtgcttt ctccgaaaac aacccctttc cactcttcat
    36361 ttgcttcttc agattgcttt tttgtctttt cattttctga ttctttcatc ataggatcat
    36421 ttccttcttc cgtatccata tcttcactag ttcttggtat ttcctccatt gttgacatcc
    36481 cttcaatgtt cacgtgtgca ttttcttcgt tcacagactg ttgttcgtta gcagcaactt
    36541 caacgacaga aattaaaacc ggggtactga gcataaggag attcatcatt agcgcactgt
    36601 attttatacg tttttttaac aagtgtattc ctcccgtaac aaattagaaa gcgtttactt
    36661 aaagtattac ctaatttatt tttactgtca ataaaaaaca aaattataat aagtgtattt
    36721 tataaaaaga aaaaatataa cttaagttgt tgataataaa gagatttatt aacggaaaaa
    36781 gaataagttt ttttaaaata acgtataaaa aagagcataa aacaaagtcg tttgttttat
    36841 gctcttcttt tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa
    36901 tttctgcgcc tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga
    36961 aacttgccac ggaatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg
    37021 ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc
    37081 gttctcgcaa acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta
    37141 tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca
    37201 attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa
    37261 aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa
    37321 acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca
    37381 gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct
    37441 tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc
    37501 tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat
    37561 tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc
    37621 ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg
    37681 cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc
    37741 ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat
    37801 tcctggtaat tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac
    37861 tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc
    37921 tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc
    37981 taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga
    38041 aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat
    38101 ttttacttct tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt
    38161 ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga
    38221 tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt
    38281 gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg
    38341 ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg
    38401 tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta
    38461 ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat
    38521 caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac
    38581 ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat
    38641 atgaaggact tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga
    38701 cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg
    38761 tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt
    38821 tcgaaattgt ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc
    38881 gccaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc
    38941 tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc
    39001 aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt
    39061 ttcatttttc aatttttctc aaaaactcct tattagttta ccatagataa accttagatt
    39121 ttctctaaat atagaaaatc cttctttagt ccgatactct ttctagtagt ccgtatgcta
    39181 aaataagtat gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag
    39241 ggtttattat gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct
    39301 ttgtccgaaa caatagtttt tcttttgttc taggactttt attgataggt gtagttttag
    39361 ttttgtgggg actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac
    39421 tgactgacga acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat
    39481 tttttcgaca aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc
    39541 agcagacagc caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag
    39601 ctttattcaa agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt
    39661 atacgcatct gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg
    39721 agatcaaaaa caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc
    39781 tgtcagcttt aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg
    39841 acgtagagat atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac
    39901 aaaaggatta actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct
    39961 gtttcgagta ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca
    40021 aggaggaatt ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg
    40081 atacacttga agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt
    40141 cacaacaagc ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca
    40201 aactaccggc ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg
    40261 atgcacagtc tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac
    40321 aatcgatgtt gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg
    40381 aactgatgta tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca
    40441 tcttccagcg tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc
    40501 aaaagattgg tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc
    40561 tgctaaaaga taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg
    40621 cactgaatat ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca
    40681 ttccagaagc gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca
    40741 atgattacac gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta
    40801 gacagatcac gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct
    40861 tggctgaaaa gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg
    40921 tgatcgcatt gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg
    40981 aaacaacaaa cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa
    41041 cagcgaaaga aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg
    41101 atttgatcga aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc
    41161 gtcatgcaga agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt
    41221 taacccaata gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac
    41281 aactgttgat gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc
    41341 attcatcttg ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac
    41401 gataccgaac acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa
    41461 gatggagacg attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt
    41521 gccaaacatt tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc
    41581 catgatattc gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc
    41641 tgccacacca gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt
    41701 cataaactta ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata
    41761 catcatcgag atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc
    41821 cactgttcca tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc
    41881 agtatacgga acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat
    41941 cagtatcgaa ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc
    42001 tgccattgca acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag
    42061 cacaccggaa aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat
    42121 tcctacaatg ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa
    42181 acgtaatgct ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac
    42241 cgcaatcaag tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg
    42301 aagttcggcc cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac
    42361 agaggaaacc gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa
    42421 ttcaccaaat acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc
    42481 aaattctgcg aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag
    42541 tgacagtgct ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt
    42601 accgatgatc gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat
    42661 ctcactcgta tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc
    42721 ccattttagg ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc
    42781 taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc
    42841 gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa
    42901 gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca
    42961 taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc
    43021 aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc
    43081 caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa
    43141 tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc
    43201 atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta
    43261 tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa
    43321 aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc
    43381 atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa
    43441 aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc
    43501 accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa
    43561 accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct
    43621 ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt
    43681 tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata
    43741 aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt
    43801 cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac
    43861 catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga
    43921 tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga
    43981 tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg aagataaaag
    44041 cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat
    44101 ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc
    44161 catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt
    44221 gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt
    44281 caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt
    44341 catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca
    44401 ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt
    44461 ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt
    44521 ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc
    44581 cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc
    44641 acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat
    44701 ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc
    44761 ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa
    44821 ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc
    44881 tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag
    44941 acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc
    45001 aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta
    45061 agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt
    45121 cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct
    45181 ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc
    45241 atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc
    45301 tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc
    45361 ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa
    45421 aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt
    45481 ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta
    45541 atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta
    45601 ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc
    45661 gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat aaagatggta
    45721 tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt
    45781 cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc
    45841 ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac
    45901 gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat
    45961 ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga
    46021 atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc
    46081 taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata
    46141 gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac
    46201 cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag
    46261 cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg
    46321 ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc
    46381 tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa
    46441 ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt tcattttctt
    46501 caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt
    46561 ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca
    46621 tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg
    46681 acccgcgttt catattcggt actctttctg gccctggatt ttctactcct acgatctgaa
    46741 tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg
    46801 gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc
    46861 cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg
    46921 cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa
    46981 atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt
    47041 cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg
    47101 gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt
    47161 tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta
    47221 aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt
    47281 cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc
    47341 cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac
    47401 caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga
    47461 cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt
    47521 ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc
    47581 taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat
    47641 acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca
    47701 ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat
    47761 gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt
    47821 ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa
    47881 tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa
    47941 tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca
    48001 aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt
    48061 caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac
    48121 cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga
    48181 ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc
    48241 ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca
    48301 gcacctaaca ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc
    48361 gggaatacgg accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt
    48421 ttgtccgttg tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca
    48481 gagactggaa ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat
    48541 tgtggttggc cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt
    48601 gcttcatcca tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa
    48661 ggcaagaaac gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca
    48721 tttttgtcat tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta
    48781 gcaggagcca tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca
    48841 gcaccttcta atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag
    48901 tatggatctc ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg
    48961 gttgattctg tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg
    49021 atatcaacat ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca
    49081 tcccgatctt ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca
    49141 accgtatctt tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt
    49201 agctgacgga tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc
    49261 atgttttcag cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc
    49321 gctccattta gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag
    49381 atagccaagc catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc
    49441 atccgtttat gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca
    49501 tgtggaagaa ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac
    49561 agctttttca ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg
    49621 ctcctgcaag cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc
    49681 ctgtagcagg caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat
    49741 ttgtctccaa aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt
    49801 ttggataata cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat
    49861 cattttctat gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt
    49921 ttctatagct gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg
    49981 aagcattgaa tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat
    50041 actctcctgc actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat
    50101 tcatactgtc aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta
    50161 cttgtagtac accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga
    50221 tgaccgtatc ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat
    50281 cagatagccg atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca
    50341 gatgcagagg ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat
    50401 agaagtatcc gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt
    50461 atgctcgata cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat
    50521 ctaatgtatg gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat
    50581 tgtaagcagt gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt
    50641 gccaattttc ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc
    50701 gcaagatcca tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa
    50761 tgattttgta ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg
    50821 gctctccgat tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat
    50881 ctactgaata gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc
    50941 ctttcgctac tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact
    51001 ctgcgccatt gattggcata ttactgcctt caattagttt attgatattc atgtggattg
    51061 gcttgaattc atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta
    51121 tgttaggctc ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat
    51181 ctttaccttc cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt
    51241 gacctaacat ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat
    51301 ctactaactg ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg
    51361 ctccagtttc atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg
    51421 tagttttact tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg
    51481 caaattcgac tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac
    51541 ggcctttatg ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat
    51601 cattctcata atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc
    51661 cgtcttgagc tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac
    51721 ctaaagaaat cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag
    51781 tcgccactac ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt
    51841 ggtacttcat gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc
    51901 cagctccaaa tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg
    51961 ttctcttgaa ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg
    52021 cattgtaata tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat
    52081 ttcgtccaat agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa
    52141 tggagaagat acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa
    52201 ctgtgttgat cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag
    52261 aagaaccatc accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac
    52321 gatagtgtac atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat
    52381 gagtaaaccc tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt
    52441 cattatcttc agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc
    52501 tcgtacgata acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa
    52561 cgattaggat tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat
    52621 agccattttt aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg
    52681 tattctcact tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga
    52741 tttgctcatg gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa
    52801 tatccagact cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg
    52861 cagcattttt agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat
    52921 ctagaacaaa aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta
    52981 gatagacatt gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg
    53041 gcagaacagg ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt
    53101 caaaattccg ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt
    53161 cgcctgcagt tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt
    53221 ttgtataatc actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt
    53281 gatagcgttc attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa
    53341 acttcgggta gcgatgctgt tcgttttcaa cttctccgct aggatagata tattcatagg
    53401 ggtcttgcgt agctgttggc ccaagacgct ccaaaatacc gttgaataac tggcgttgaa
    53461 tgcgctggag tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc atctcatttt
    53521 cttcgctacg ttgaatttca gcggaatctt ctgtatcagt ttcattttct tgtaattctt
    53581 ggttatctat tctttcatca accaccactt gaagaggtag ttacccttcg ttcacatcgg
    53641 ggatcttttc tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa taaacttttc
    53701 cgacatacca ttcttgacca tcgatcgtct cgacttttag gtcttctcca tcttcatcat
    53761 aggaggatag acctgttcca cgaacgtttt ttacggtacc aatcccggcg gctgccgcat
    53821 cgattgctaa acgtattcta ccttcattgg cactttcctt tttttcaaaa gccagatgcc
    53881 attcaagggt ttcttctttt tcaatatact tcccttcaat ttttgcatag tcctcatcta
    53941 agaatacctc atcctccttt gtttctgttg actcagcagc tacatccagg gtcgtcatat
    54001 gatagactga ctggaaatat ggtccaaata gcaaactgac agttactaac tgcaaaagct
    54061 tcaattttaa ccctttcaca tccttatact cctttcaaaa atatttgtct tctttcacaa
    54121 caatcaacaa aacaatgccc atctaattat aatttaattt aattttaatt ttaaatcaaa
    54181 tgatat
//
LOCUS       DAYYBG010000010        53719 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000010 DAYYBG010000000
VERSION     DAYYBG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.010"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..840
                     /locus_tag="ACHAUN_000566"
     CDS             160..840
                     /locus_tag="ACHAUN_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8459296.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="ACHAUN_000567"
     CDS             930..1964
                     /locus_tag="ACHAUN_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8459297.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="ACHAUN_000568"
     CDS             1978..2313
                     /locus_tag="ACHAUN_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459298.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="ACHAUN_000569"
     CDS             2297..4777
                     /locus_tag="ACHAUN_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459299.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="ACHAUN_000570"
     CDS             4833..6392
                     /locus_tag="ACHAUN_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8459300.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="ACHAUN_000571"
     CDS             6389..7753
                     /locus_tag="ACHAUN_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8459301.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="ACHAUN_000572"
     CDS             7924..8994
                     /locus_tag="ACHAUN_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459302.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="ACHAUN_000573"
     CDS             9009..9677
                     /locus_tag="ACHAUN_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459303.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="ACHAUN_000574"
     CDS             9697..10275
                     /locus_tag="ACHAUN_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8459304.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="ACHAUN_000575"
     CDS             10676..11587
                     /locus_tag="ACHAUN_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8459305.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACHAUN_000576"
     CDS             complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACHAUN_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8459306.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="ACHAUN_000577"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="ACHAUN_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8459307.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="ACHAUN_000578"
     CDS             13579..14127
                     /locus_tag="ACHAUN_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8459308.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="ACHAUN_000579"
     CDS             14242..16602
                     /locus_tag="ACHAUN_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8459309.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="ACHAUN_000580"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="ACHAUN_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8459310.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="ACHAUN_000581"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="ACHAUN_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8459311.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="ACHAUN_000582"
     CDS             19108..20007
                     /locus_tag="ACHAUN_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459312.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="ACHAUN_000583"
     CDS             20000..21262
                     /locus_tag="ACHAUN_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459313.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="ACHAUN_000584"
     CDS             21423..23321
                     /locus_tag="ACHAUN_000584"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8459314.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="ACHAUN_000585"
     CDS             complement(23651..24370)
                     /locus_tag="ACHAUN_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8459315.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    24560..24607
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24707..25039
                     /locus_tag="ACHAUN_000586"
     CDS             24707..25039
                     /locus_tag="ACHAUN_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459316.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="ACHAUN_000587"
     CDS             25026..27029
                     /locus_tag="ACHAUN_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8459317.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="ACHAUN_000588"
     CDS             27048..27518
                     /locus_tag="ACHAUN_000588"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8459318.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="ACHAUN_000589"
     CDS             27539..28123
                     /locus_tag="ACHAUN_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8459319.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="ACHAUN_000590"
     CDS             28138..29124
                     /locus_tag="ACHAUN_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8459320.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="ACHAUN_000591"
     CDS             29114..29425
                     /locus_tag="ACHAUN_000591"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8459321.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="ACHAUN_000592"
     CDS             29447..31228
                     /locus_tag="ACHAUN_000592"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8459322.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="ACHAUN_000593"
     CDS             31221..32597
                     /locus_tag="ACHAUN_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8459323.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="ACHAUN_000594"
     CDS             32604..33239
                     /locus_tag="ACHAUN_000594"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8459324.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="ACHAUN_000595"
     CDS             33269..34624
                     /locus_tag="ACHAUN_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8459325.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="ACHAUN_000596"
     CDS             complement(34816..36210)
                     /locus_tag="ACHAUN_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8459326.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="ACHAUN_000597"
     CDS             36302..37273
                     /locus_tag="ACHAUN_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8459327.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="ACHAUN_000598"
     CDS             37270..38052
                     /locus_tag="ACHAUN_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8459328.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="ACHAUN_000599"
     CDS             complement(38221..38592)
                     /locus_tag="ACHAUN_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8459329.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="ACHAUN_000600"
     CDS             38726..39169
                     /locus_tag="ACHAUN_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8459330.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACHAUN_000601"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACHAUN_000601"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8459331.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="ACHAUN_000602"
     CDS             complement(40040..41407)
                     /locus_tag="ACHAUN_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8459332.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="ACHAUN_000603"
     CDS             complement(41391..42221)
                     /locus_tag="ACHAUN_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8459333.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="ACHAUN_000604"
     CDS             42384..42821
                     /locus_tag="ACHAUN_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8459334.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /gene="rpoE"
                     /locus_tag="ACHAUN_000605"
     CDS             42897..43517
                     /gene="rpoE"
                     /locus_tag="ACHAUN_000605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8459335.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="ACHAUN_000606"
     CDS             43706..44212
                     /locus_tag="ACHAUN_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8459336.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="ACHAUN_000607"
     CDS             44229..45128
                     /locus_tag="ACHAUN_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8459337.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="ACHAUN_000608"
     CDS             45215..46099
                     /locus_tag="ACHAUN_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8459338.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46291..46362)
                     /locus_tag="ACHAUN_000609"
     tRNA            complement(46291..46362)
                     /locus_tag="ACHAUN_000609"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46327..46329),aa:Arg,seq:ccg)
     gene            46422..47756
                     /gene="rpoN"
                     /locus_tag="ACHAUN_000610"
     CDS             46422..47756
                     /gene="rpoN"
                     /locus_tag="ACHAUN_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8459339.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47905..48996
                     /locus_tag="ACHAUN_000611"
     CDS             47905..48996
                     /locus_tag="ACHAUN_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8459340.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            49037..50038
                     /gene="gap"
                     /locus_tag="ACHAUN_000612"
     CDS             49037..50038
                     /gene="gap"
                     /locus_tag="ACHAUN_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8459341.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50148..51338
                     /gene="pgk"
                     /locus_tag="ACHAUN_000613"
     CDS             50148..51338
                     /gene="pgk"
                     /locus_tag="ACHAUN_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8459342.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51442..52197
                     /gene="tpiA"
                     /locus_tag="ACHAUN_000614"
     CDS             51442..52197
                     /gene="tpiA"
                     /locus_tag="ACHAUN_000614"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8459343.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52313..53611
                     /gene="eno"
                     /locus_tag="ACHAUN_000615"
     CDS             52313..53611
                     /gene="eno"
                     /locus_tag="ACHAUN_000615"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8459344.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17827 a         9355 c        11351 g        15186 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttagttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgacttgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta
    46261 ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc
    46321 aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt
    46381 aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt
    46441 tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca
    46501 tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg
    46561 aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt
    46621 catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt
    46681 ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga
    46741 aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag
    46801 aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga
    46861 ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc
    46921 aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg
    46981 aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg
    47041 ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg
    47101 accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa
    47161 atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact
    47221 tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac
    47281 aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg
    47341 ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc
    47401 ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg
    47461 cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca
    47521 ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga
    47581 agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga
    47641 aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat
    47701 atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag
    47761 tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca
    47821 aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat
    47881 agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt
    47941 ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat
    48001 attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg
    48061 attggacgtc gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa
    48121 acagatcttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca
    48181 actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg
    48241 catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca
    48301 ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc
    48361 ctagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg
    48421 gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc
    48481 cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga
    48541 atggctgcta aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg
    48601 gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt
    48661 gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag
    48721 atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc
    48781 tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg
    48841 caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa
    48901 gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct
    48961 gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat
    49021 aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg
    49081 tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt
    49141 gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa
    49201 cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt
    49261 agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga
    49321 atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa
    49381 acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa
    49441 ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg
    49501 tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac
    49561 aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga
    49621 cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa
    49681 agctatcggt ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt
    49741 tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt
    49801 tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga
    49861 cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca
    49921 aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa
    49981 cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga
    50041 tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc
    50101 gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa
    50161 cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc
    50221 ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca
    50281 actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa
    50341 cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat
    50401 taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc
    50461 gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg
    50521 gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg
    50581 tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg
    50641 cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg
    50701 gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt
    50761 ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg
    50821 gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag
    50881 aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct
    50941 tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg
    51001 aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat
    51061 ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg
    51121 aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag
    51181 gcgcaacaac aatcatcggt ggcggagact cagctgcagc agcagaacaa ttaggatttg
    51241 ctgacaaatt cacacacatc tcaacaggtg gtggcgcaag cttggaattg cttgaaggta
    51301 aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct
    51361 gacgagtaat cgatcaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata
    51421 aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac
    51481 aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat
    51541 gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca
    51601 gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg
    51661 ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc
    51721 ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagca
    51781 aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc tttagaaacc
    51841 tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat
    51901 ctttcagctg aacaagtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt
    51961 actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca
    52021 gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct
    52081 gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc
    52141 ggtggcgcaa gcttgcaagc agattcattt ttgtcattgt tagacgctgt aaaataattg
    52201 ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc
    52261 gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat
    52321 tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt
    52381 agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac
    52441 tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg
    52501 tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga
    52561 tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa
    52621 aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc
    52681 tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc
    52741 aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca
    52801 agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc
    52861 tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg
    52921 tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat
    52981 cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc
    53041 tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg actcaggcga
    53101 aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc
    53161 aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca agaaattaac
    53221 tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca
    53281 aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca
    53341 aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac
    53401 agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt
    53461 tgcaacaaat gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa
    53521 atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt
    53581 gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac
    53641 tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa
    53701 atatcagcag gtagagagt
//
LOCUS       DAYYBG010000011        50101 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000011 DAYYBG010000000
VERSION     DAYYBG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.011"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..295)
                     /locus_tag="ACHAUN_000616"
     CDS             complement(<1..295)
                     /locus_tag="ACHAUN_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8459345.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYG"
     gene            complement(297..494)
                     /locus_tag="ACHAUN_000617"
     CDS             complement(297..494)
                     /locus_tag="ACHAUN_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8459346.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(888..2615)
                     /locus_tag="ACHAUN_000618"
     CDS             complement(888..2615)
                     /locus_tag="ACHAUN_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8459347.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2608..3801)
                     /locus_tag="ACHAUN_000619"
     CDS             complement(2608..3801)
                     /locus_tag="ACHAUN_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8459348.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3988..4632)
                     /locus_tag="ACHAUN_000620"
     CDS             complement(3988..4632)
                     /locus_tag="ACHAUN_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8459349.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4726..4860)
                     /locus_tag="ACHAUN_000621"
     CDS             complement(4726..4860)
                     /locus_tag="ACHAUN_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8459350.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4862..6994)
                     /gene="feoB"
                     /locus_tag="ACHAUN_000622"
     CDS             complement(4862..6994)
                     /gene="feoB"
                     /locus_tag="ACHAUN_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8459351.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6991..7464)
                     /locus_tag="ACHAUN_000623"
     CDS             complement(6991..7464)
                     /locus_tag="ACHAUN_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8459352.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7865..8089
                     /gene="nrdH"
                     /locus_tag="ACHAUN_000624"
     CDS             7865..8089
                     /gene="nrdH"
                     /locus_tag="ACHAUN_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8459353.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8248..10407
                     /gene="nrdE"
                     /locus_tag="ACHAUN_000625"
     CDS             8248..10407
                     /gene="nrdE"
                     /locus_tag="ACHAUN_000625"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8459354.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10457..11422
                     /gene="nrdF"
                     /locus_tag="ACHAUN_000626"
     CDS             10457..11422
                     /gene="nrdF"
                     /locus_tag="ACHAUN_000626"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8459355.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11511..12650)
                     /locus_tag="ACHAUN_000627"
     CDS             complement(11511..12650)
                     /locus_tag="ACHAUN_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8459356.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12959..13672)
                     /locus_tag="ACHAUN_000628"
     CDS             complement(12959..13672)
                     /locus_tag="ACHAUN_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8459357.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13926..14627)
                     /gene="nagB"
                     /locus_tag="ACHAUN_000629"
     CDS             complement(13926..14627)
                     /gene="nagB"
                     /locus_tag="ACHAUN_000629"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8459358.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14861..16393
                     /locus_tag="ACHAUN_000630"
     CDS             14861..16393
                     /locus_tag="ACHAUN_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8459359.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16584..17786)
                     /locus_tag="ACHAUN_000631"
     CDS             complement(16584..17786)
                     /locus_tag="ACHAUN_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8459360.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17868..18557)
                     /locus_tag="ACHAUN_000632"
     CDS             complement(17868..18557)
                     /locus_tag="ACHAUN_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8459361.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18663..19031)
                     /gene="rplL"
                     /locus_tag="ACHAUN_000633"
     CDS             complement(18663..19031)
                     /gene="rplL"
                     /locus_tag="ACHAUN_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8459362.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19084..19587)
                     /gene="rplJ"
                     /locus_tag="ACHAUN_000634"
     CDS             complement(19084..19587)
                     /gene="rplJ"
                     /locus_tag="ACHAUN_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8459363.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19791..19918)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19997..20686)
                     /gene="rplA"
                     /locus_tag="ACHAUN_000635"
     CDS             complement(19997..20686)
                     /gene="rplA"
                     /locus_tag="ACHAUN_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8459364.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20790..21212)
                     /gene="rplK"
                     /locus_tag="ACHAUN_000636"
     CDS             complement(20790..21212)
                     /gene="rplK"
                     /locus_tag="ACHAUN_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8459365.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21555..22223
                     /gene="sdaAB"
                     /locus_tag="ACHAUN_000637"
     CDS             21555..22223
                     /gene="sdaAB"
                     /locus_tag="ACHAUN_000637"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8459366.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22236..23108
                     /gene="sdaAA"
                     /locus_tag="ACHAUN_000638"
     CDS             22236..23108
                     /gene="sdaAA"
                     /locus_tag="ACHAUN_000638"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8459367.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23199..24299)
                     /locus_tag="ACHAUN_000639"
                     /pseudo
     CDS             complement(23199..24299)
                     /locus_tag="ACHAUN_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24391..25278)
                     /locus_tag="ACHAUN_000640"
     CDS             complement(24391..25278)
                     /locus_tag="ACHAUN_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8459368.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25355..25900)
                     /gene="nusG"
                     /locus_tag="ACHAUN_000641"
     CDS             complement(25355..25900)
                     /gene="nusG"
                     /locus_tag="ACHAUN_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8459369.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26001..26171)
                     /gene="secE"
                     /locus_tag="ACHAUN_000642"
     CDS             complement(26001..26171)
                     /gene="secE"
                     /locus_tag="ACHAUN_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8459370.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26195..26347)
                     /gene="rpmG"
                     /locus_tag="ACHAUN_000643"
     CDS             complement(26195..26347)
                     /gene="rpmG"
                     /locus_tag="ACHAUN_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8459371.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26509..27153
                     /gene="cbpA"
                     /locus_tag="ACHAUN_000644"
     CDS             26509..27153
                     /gene="cbpA"
                     /locus_tag="ACHAUN_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8459372.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27209..27712)
                     /locus_tag="ACHAUN_000645"
     CDS             complement(27209..27712)
                     /locus_tag="ACHAUN_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8459373.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27725..27771)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28309..29274
                     /locus_tag="ACHAUN_000646"
     CDS             28309..29274
                     /locus_tag="ACHAUN_000646"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8459374.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29373..30545)
                     /locus_tag="ACHAUN_000647"
     CDS             complement(29373..30545)
                     /locus_tag="ACHAUN_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459375.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30532..31677)
                     /locus_tag="ACHAUN_000648"
     CDS             complement(30532..31677)
                     /locus_tag="ACHAUN_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459376.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31691..32615)
                     /locus_tag="ACHAUN_000649"
                     /pseudo
     CDS             complement(31691..32615)
                     /locus_tag="ACHAUN_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32732..33811
                     /locus_tag="ACHAUN_000650"
     CDS             32732..33811
                     /locus_tag="ACHAUN_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8459377.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33808..34436
                     /locus_tag="ACHAUN_000651"
                     /pseudo
     CDS             33808..34436
                     /locus_tag="ACHAUN_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34546..34773)
                     /locus_tag="ACHAUN_000652"
     CDS             complement(34546..34773)
                     /locus_tag="ACHAUN_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8459378.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34876..37464)
                     /locus_tag="ACHAUN_000653"
     CDS             complement(34876..37464)
                     /locus_tag="ACHAUN_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8459379.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37484..38143)
                     /locus_tag="ACHAUN_000654"
     CDS             complement(37484..38143)
                     /locus_tag="ACHAUN_000654"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8459380.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38192..38695)
                     /gene="trmL"
                     /locus_tag="ACHAUN_000655"
     CDS             complement(38192..38695)
                     /gene="trmL"
                     /locus_tag="ACHAUN_000655"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8459381.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38916..39782)
                     /locus_tag="ACHAUN_000656"
     CDS             complement(38916..39782)
                     /locus_tag="ACHAUN_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8459382.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39891..40532
                     /locus_tag="ACHAUN_000657"
     CDS             39891..40532
                     /locus_tag="ACHAUN_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8459383.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40594..41955)
                     /gene="mgtE"
                     /locus_tag="ACHAUN_000658"
     CDS             complement(40594..41955)
                     /gene="mgtE"
                     /locus_tag="ACHAUN_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8459384.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41973..42872)
                     /locus_tag="ACHAUN_000659"
     CDS             complement(41973..42872)
                     /locus_tag="ACHAUN_000659"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8459385.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42874..43671)
                     /locus_tag="ACHAUN_000660"
     CDS             complement(42874..43671)
                     /locus_tag="ACHAUN_000660"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8459386.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43649..44353)
                     /locus_tag="ACHAUN_000661"
     CDS             complement(43649..44353)
                     /locus_tag="ACHAUN_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8459387.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44484..45060
                     /locus_tag="ACHAUN_000662"
                     /pseudo
     CDS             44484..45060
                     /locus_tag="ACHAUN_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45178..45855
                     /locus_tag="ACHAUN_000663"
     CDS             45178..45855
                     /locus_tag="ACHAUN_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8459388.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45934..47742)
                     /gene="pepF"
                     /locus_tag="ACHAUN_000664"
     CDS             complement(45934..47742)
                     /gene="pepF"
                     /locus_tag="ACHAUN_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8459389.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47918..48916)
                     /locus_tag="ACHAUN_000665"
     CDS             complement(47918..48916)
                     /locus_tag="ACHAUN_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8459390.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49142..49795)
                     /locus_tag="ACHAUN_000666"
     CDS             complement(49142..49795)
                     /locus_tag="ACHAUN_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8459391.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15002 a        10511 c         8781 g        15807 t
ORIGIN      
        1 ctccataatc tgctcgatat aaaccattta ttccagcagt tcttccaatt tcactcaaat
       61 ttcttattaa ttcctcttga tattcagtga atcttatttt attagtataa gcagtaaaat
      121 atagataatt caccaatcta tttattaaag gaccgggatc taattcatat ttttgttttt
      181 ctaattttaa atataccttt tgtaaaacct caagaatatc agaatattta ggatctttac
      241 ttatagatat atcattatat aattcatgaa caatttgctt agcatttttt ttcattttag
      301 cacttccctg gaattgctcc acctaaaaaa ccacctatag acattcctgc aatacaagta
      361 gttgccttgg cccaatcgac cgtacattta tttttagtgc aatacactcc atttccataa
      421 tattttccac tatgagtggt acccccatag ataagttgtg tctctttaat agacaaaatt
      481 tttaaatgtt tcataatcta atcaccccta aattaatgtt tttagactat tattctaaca
      541 ttcattaaat ttcaaaaaac aattccttct tgaaacgtag caaaataatt cctgaataag
      601 aatttaatca cttttaagta tataaactat gtatattctt taatattttt tgagaatgca
      661 cttcgtacat aataaacttt aatcacatta gtctttcata cctacgatcg atctctttga
      721 ctcgcaccca taaattcata atagtttgtc ggcgtctttg gacgtttcga agatgagcta
      781 cgcattctct ttttttatag acaaaaaaga tagagacgaa catggagctt ccaaatttgg
      841 atatttgatc caattttgga agctccattt atttcgtgtc tgtcttttta ttttttccag
      901 ctttcgacta attcttggtt ctctttgatc cagtcttttg ctgcttgctg tggatctttt
      961 ccattgttga tttccaacat aactttttcc atatcttttt ctgaccaatg gaaattgtct
     1021 aatactttgt aagcttctgg ctggtcttct ttcaatcctt ttcgaaccat cgtatggatc
     1081 tgctcttcgc ttcccattcc gttttcagga tcttctaagt atttcagatc atatttggca
     1141 aacatccaat gaggtgtcca tcctgtaact acaattggtt cgtgtttttt gatcgcttga
     1201 cctagtgcaa cggtcattgc tcctgaagaa gatgtctcta ctttccagtc tttcaagttg
     1261 tcatattttt ggatcgtgtt ttcagcagcc gtaactacac cagctcctgg ctcgattcct
     1321 atgattttct tacctgcctg atcggttaac tcatcgatcg aatttacatc catgtaggct
     1381 ggcactgcta aaccaacttt tgctcctgtc aaattggcac ctaggtcttc gacttgatct
     1441 ttatattgtg catattgtga accatgagtt ttaggtagcc aagctgaaac catggcatct
     1501 gactcgccat ttgaaacaga tttccacatg attgaattat ctaatggcgt catcgttaca
     1561 tcatagccca tttgtttcaa tacttcaccg actacatttg ttgaagcaac ttctgtatcc
     1621 cattcaacat aagataagtt gatacgtttt gtttctttgg cagatgaaaa actccctata
     1681 ccaatcgcac ctaagatcac tactactaca gcaccaatga tcagtccttg acgtttttta
     1741 cttttctgtg ttgttgctcc tgcagctttt ttattgtttg gctggttcaa atgctgtgta
     1801 aaacggtcaa tgatgattgc taagataact aatgcaaccc cgttgacaaa tccattcccg
     1861 acttgtgctc tttgcaatgc tgataatacg ccgcgtccta agccgggtgc accgatcata
     1921 gaggcaataa cgaccattga aagtgacagc attgttgttt gattgatccc tgccatgatc
     1981 gtacttttag ctaaaggcaa ttctagtttg aatagttttt gacgacttgt gctcccgaat
     2041 gaatcagaag cttctactaa ttctttagga acttgtcgaa tccctaaatt cgtaaaacga
     2101 acggttggtg gcagagcaaa gatgactgag gcaaataccc ctggaaccat cccgataccg
     2161 aaaaatgcaa ctgctggaat caaataaacg aatcctggca ttgtctgcat aaagtcaagg
     2221 atcggtttga taatactttg ggctttactg ctttttgcca tcaagatccc taatggtacc
     2281 ccaatgatga tcgagatcac actagataaa agaacaagtg tgacagtatt catcaaatca
     2341 ttccatagac cttggttata aataaaccat agaccgatta atgtgaacaa cgtaagtcct
     2401 ggacgtttct tagaaataaa gaaagctgct accgtcaata acaagataaa caaaggagct
     2461 ggaatcacta acagtaaatt tgtaatcccc gacatcactg cctgtccaat cgtttgtaaa
     2521 aatgaaaata ggccagaaaa agttgtcgta aaccattctg tgatattttc tacccagttt
     2581 gccactggta attgatagtt caatagatta ttcatgttca ctcacctcgc ttgtttctgc
     2641 taatgcttca atcacactac ctcggatgac gacacctttt aattttccat cctctacaac
     2701 tgcaagtgga gtcggtgagt cataaataat attgaaaata tcacttacca ccatgtcttt
     2761 cccaatcacc gtcacatttt catctaggaa gtcgatcaat ggacgttttt gcttcctagc
     2821 ttctaacgct tgatctgcag tgatgatccc tttcaggtgt ctctttctgt caacagccat
     2881 cagcatgctg acttcttcat tacgcatcct tgtcaatgca acgtttggtc catcagattc
     2941 gatattcgtc gtcaatgcag gtaccatgat gttttgagcc gtaagcactt tggaacgatc
     3001 gacttcttcc acaaattcac gtacataatc atttgctgga ttggtcaata tttcttcacc
     3061 tgttccgatc tgcatgattt cgccgtcttt catcaaggcg attctgtccc caatccgtaa
     3121 agcttcattc aaatcatggg taatgaaaat aatcgttttt tgaacattgg cttgcaggtc
     3181 cagtaattcg tcttgcattt ctcgccggat cagcggatca agagccgaga acgcttcgtc
     3241 catcagcaag atctcaggat catttgctaa agctctggct aggccaaccc gttgttgcat
     3301 cccaccagat aactgactag gatattggtc tttaaatgaa agcaaactag aattttctaa
     3361 tgctttttct gctttttctt gtcgttcttc tttaggtaca ccacggactt ctaacccgta
     3421 ttctgtattt tccaagatcg ttcgatgtgg aaacagaccg aagttttgga aaaccatatt
     3481 gattttatga cgacggactt ctcgcaattc ttctttgctt aacttcgata catcttctcc
     3541 atcaatatag atactgcccg aagttggctc aatcagacga ttcaatagac ggatcaatgt
     3601 ggatttccca ctcccggaaa gccccatgat cacgaagatt tctccttctt ttacgtcaaa
     3661 attcacgtca tacacaccaa ccgttgcacc ggttttcttt aaaatctccg ttttcggctg
     3721 gtgatcattc atcatatcta aagcggcttg tgtcttttta ccaaatatct ttgttaaatg
     3781 ttttacttct acttttacca aacaaattct ccttttttat gtaaattcat ttttctttct
     3841 gaaaaaagtg accacactat gttaacatca tgtagtcact ttgtcaaaaa tgaaagaaat
     3901 ttgcttgtcc aaactgaaaa aaacttggct aaatctttgt aaataaaagg tttagccaag
     3961 ttaatatttt tttaatattt cacacaatca ttctgcaagc tgatgctccg ggtaaaaaaa
     4021 gtttgtcata cgctgtagtg tatattcatt tccaataaaa tgaacgatgt cccctgccgt
     4081 aaatttagca taggggccag gtgaaacaag cagttcttca ttatgagaaa ttgcgataac
     4141 tgtggcgcca gtcgcctgcc agatattcaa ctcgctaatt gaacggtcca gatgcattgc
     4201 tttttctgat aaagataatt caaacggaag taaaggattc atcttatgta ttcgcttcgt
     4261 ttgattgacc agctgatcca ataattgaga aaaatgattc agctcatctt gttgcttctc
     4321 cacacttgcc atgatttctg cttttgtatc ttggatggat tgtacatctt gatattgcaa
     4381 taggaaatct tttgcttttt ccttcgaagc aacaaatgcc ccacttccat gccgtacttc
     4441 catgattcca agatctacca gcacattgat agccttgcgg gtagtctcag gagaagcatt
     4501 gtacgtactg gcaagtgttg agcgggcatg gatcttctca cccactttat atcggttttc
     4561 aacgatacgt tcggctaaat caacagcaat ctgctggtag cgcgggcgga cgattttttt
     4621 ctttccagac attttctttc ctctttcttt ttttactttc cctcatctta ctatgctcta
     4681 gcaaaataag caaaaaaggg gagaatagcc tcttgcaacg actgatcatc gcttgatctg
     4741 ttcctttttt actgggcaag ctgtttgaca gtcatcacaa ttcttaccgg attttaaacg
     4801 agtatagaca attgcgcctg cacttccaaa gattaacaca cttaataaga atgtcgccat
     4861 cctattcctc tccttctaat gacaatatag tataaaactg gttcttggat tttggctgac
     4921 ggaacaaaac ataaacgagt cctatcatca gtaaaatagc aagatacgtc cctaacgcta
     4981 cctgtccacc ctccaataag tgtcccaact gataaatcac aaaactaact aaataagcta
     5041 aaccgcattg atagcctatg gcgatccaag tccatttctt ggcattcatt tcccgatgga
     5101 tagccccaat tgccgcaaaa caaggcgcac aaagtaaatt gaaggctaac aatgaataag
     5161 ctgctgcggg agtatatgtg tgttgtagca cttgccagat ttctctgcca ttttcggata
     5221 cttccgcatg accaaacaaa ataccaaatg tgccaataat attttcttta gcgaccaatc
     5281 ctgtaatcgc tgcgactgtt ccttgccagt ttccccaacc aagcggctga aaaacaactg
     5341 ccaggattcg cccgaaaaag gctaagatac tttggtcttc agaaacaggt tgagcataaa
     5401 aattataaga agagctgaac cagatcacga tactagaaac aaagataatc gttcctgctt
     5461 ttttaacaaa agacttgctg cggtcatagg tttgtcttag gaccgctgac cattgaggaa
     5521 aatgatatgc gggcaattcc atgataaaag gagctgtatc tccagcaaac aaacgtgttt
     5581 ttttcaagct gattccagag aaaataattg cagtcatacc aagaaaatag gcagaaggag
     5641 ctacccaaga ttgatgagga aaaaaggccc ctgctactaa ggcaataatc ggcaactttg
     5701 cagaacaagg catgaaggtt gtgaccatga tcgtcaaacg acgatcttgc tcgttttcaa
     5761 tcgtgcgact ggccatgacg cctggcactc cacatcctgt agcaatcagc ataggaatga
     5821 aggattttcc tgaaagaccg aatttcctga aaagtcgatc catgacaaaa gcgatcctcg
     5881 ccatatatcc gcagtcttcc agaaagctca agcataaaaa caatataatc aattgcggta
     5941 gaaatcctaa cactgctccc acgcctgcga tgatgccgtt caagatcagc tcctgcatcc
     6001 atcccgctac ttgccattca gctaacaatc tgccaacatg ttctggtaac catgtaccaa
     6061 acaatgtgtc attgatccaa tctgtcccca ttgttcctac tgtttgaata gccagataat
     6121 agatcagcca catcacaaat gcgaagatcg gcaaggctaa ccaacgattc gttgcaatct
     6181 gatcgatttt gtcactcatc gataattgga aagaatcatt tgacacaaca cagagtgtac
     6241 atagttgggt aataaattca taacgttcat tgaccagaat cgtctcacta tcgtcgtcca
     6301 atagtttctc tgttaactgg atcagttgct cgatctcttt ttgctgagaa actgctatct
     6361 cgtaatcctc tcttaccttc tgatctcgtt caaaatattt cattgcatac cagcgtttct
     6421 tgccgctttg ttctcctagt acatcttcaa tttcatgtaa agctgcttct aaacgtggat
     6481 catatgtaca gggaacagga atagaaggcc gcttttttgc agactcctga atcttttcga
     6541 ttagtttttc gattccttgc tttttcaata cgctgattgg taaaactggt acaccaagac
     6601 cataggaaag cttttctaga ttgatttttt ttccttcttt tttgatgaca tccatcatat
     6661 tcagaccaat gatcatagga atgtctaaat ccaataactg cgtagttaaa taaagatttc
     6721 gttctaaatt ggtagcatca ataatgttga tgatcagttc aggctgttcc tgaaccaaat
     6781 aatctcttgc aatcacttct tctggtgtat aaggagacaa agaatagatg ccgggtaggt
     6841 cctgaatatg aatgttcgga ttttttttca acgatccttc ttttttttct actgttacgc
     6901 ccggccaatt ccctacatat tgattcgaac cagtcaagag attgaacaag ctagttttcc
     6961 cgctgtttgg atttccgact aacgcgatat tcattctttt tcctcccttt gtatcaagat
     7021 tagttcggcc tctgactttc gtaacgtcag ttcataccct cgcactgtca gttcgattgg
     7081 atctcccaga ggggctagtt ttcggataac tatttttaca tttcgcgtca atcccatatc
     7141 cattaaccgt cgcttcactg cgccttgtcc gtggattgct acgacgatcc cagtctctcc
     7201 tgcttttaat tgatcaagag aagtccagct ttcagaggaa gcagtctttt tattgacaaa
     7261 gatttgacct agtatttccc gacttactgc cactcgatga ctttttacca gtaaaatggc
     7321 attgttgcct gataaattga cgacgacgat tttacttccg gcaacgatcc cgatattttg
     7381 caaatggcgt tttgtttttt cttctgtttc gattcgttcg acttgataaa cgaaattctg
     7441 ctttgcctgc gctaaggtct gcatattgat tcctctcttg tgaaataaat catgtattaa
     7501 aatcattcat tatatgttct aattttctta cgtgttacgc aaataaatga gaatcattct
     7561 cacatagaat actctcggta attgagaatg tcaattatac ttgtgatatt atgagcgaat
     7621 ctttagtatt gatagataaa aatgagaaaa aaatatttct tcatagtttt ccacaatttt
     7681 ttctctatta aatttgctat tttcccacac gatacttgtt ttcagctctt ttttcaaaaa
     7741 cacaaaactt agtattccac agtttccaca acacaatatg tagtgtttgt tgtttgactt
     7801 ttttactaag agttttatac tatctattat agataataag aatgtgttag aggaggcact
     7861 caccatgaac gtaaaaatct tttctaaaaa taattgtatg caatgtaaga tggtcaaacg
     7921 gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat atcgatgaac agcctgaagc
     7981 agtagactgg ttgaaagcac aaggattcca aagtgttcct gtcattactt ctgatgcaga
     8041 aacagttatc ggattccgtc ctgatcagtt gcgtaaactt gccagctaac atgactaaaa
     8101 gaatcaaaac gtgatacatg gatttgaagg gcttgagaca gcaccagttt ttgtcgcaaa
     8161 cttccaaatc cttttttaaa gcttaaaaac aatgcaatga tttgcaatca agcatcatca
     8221 aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa atttacaaga tgtcagttat
     8281 ttcaagctga ataatgaaat caatcggcca gtcaatggtc aaatcccttt gaataaggat
     8341 aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac caaatacgat gacttttcta
     8401 tctattatgg aaaaaatcaa ttacttgatc gatcaagaat atatagaagc agaattcatt
     8461 caattgtatt caccatcatt tattgaaaag ctatatcaat ttttggacga gcaacatttt
     8521 tctttcaaat cttttatggc tgcttacaaa ttttattctc aatatgcgtt gaaaaccaac
     8581 gacggttccg cttatctgga aacttatgaa gatcgagtag ccttcaatgc gctttacttt
     8641 gcgaacgggg atgaagaatt agcacttgct cttgcagacg aaatgatcca tcaacgatat
     8701 cagccagcta caccttcttt cttgaatgct ggacgtaaac gtcggggcga attggtgtct
     8761 tgtttcttga ctcaagtcac tgacgatatg aactctatcg ggcgctcaat caactctgct
     8821 ttgcaattgt cccgtattgg tggcggcgta gggattactt tatcaaatct tcgtgaagcc
     8881 ggcgctccta tcaaaggata tgagggtgct gcaagtggtg tcgttccagt catgaaatta
     8941 ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc gtcaaggagc tggcgtggtt
     9001 tatttgaacg ttttccatcc agacattatt gctttccttt cagcaaaaaa agaaaatgca
     9061 gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga tcgtacctga taaattttat
     9121 gaattgacac gtaaaaacga agatatgtat ttatttagtc catatagtgt cgaaaaagaa
     9181 tatggtgtcc cattctctta tgtaaatatt acagctgagt atgatcatct agttgcaaat
     9241 ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg aaaatgaaat ctcaaaatta
     9301 caacaagaat ctggctatcc ttacgtaatc aatattgata cagcgaaccg cgagaatcca
     9361 atcgatggca agatcatcat gagtaattta tgttcagaga ttttacaagt tcaaacgcct
     9421 tctgtgatta acggcaaaca ggaatatgaa gtattaggaa cggatatctc atgtaactta
     9481 ggctcgacaa atatcgtgaa tttgatgcaa agccctaact ttggtcaatc tgtgcgtacg
     9541 atgactcgtg cattgacatt tattacagat gcatctgata tcgatgtcgt accaccgatc
     9601 caaaacggaa ataagctgaa tcatacgatt ggattaggtg ccatgggctt acacacttat
     9661 cttgcaaaag aacaaatgga atatggctca gaggattcat tagactttac aaatatttac
     9721 ttcatgctgt tgaattattg gacacttgtt gaaagcaaca atatcgctcg tgaaagaaaa
     9781 caagttttcc ataattttga aaaatcagct tatgctgacg gcacttattt cgaaaaatac
     9841 gtaactggag aattccagcc aaaatcagaa aaagtcaaag cattgtttga aggcattttt
     9901 atcccaactg ctgaagattg gaatgctttg aaacaagcag taatgaaaga tggactgtat
     9961 catcaaaatc gcttggcagt agcaccaaat ggctcaattt catatatcaa cgatacgagt
    10021 gcaagtattc atcctatcac tcgcatgatc gaagaacgcc aagagaagaa aatcggtaaa
    10081 atttattacc cagcgcctta tctatccaat gagacgattc cgtattacac ttctgcttat
    10141 gatatggaca tgcgtaaagt aatcgatgtt tatgcaatgg cacaacaaca cgtcgatcaa
    10201 ggcatgagtt tgactttatt catgcgttct gaaatcccag aaggcattta tgaatggaag
    10261 acaacaacga agcaaacgac acgtgatttg aatattttaa gacattacgc tttccacaaa
    10321 ggaatcaaat ccatttatta cgtacgtacc tttactgatg atgcagaaga aatcggaagc
    10381 aatcaatgtg aaagctgcgt gatctaatat catagataga ttcgcttatt catattttaa
    10441 ttaaaggagc attctcatgt cagaaacata ttatgaagca ataaactgga atgaaattga
    10501 agatatcatt gataaatcca cttgggagaa gctgacagag caattttggc tagatacacg
    10561 tatcccactt tcaaatgacc tagatgattg gcgtacattg tctcaattgg aaaaagatac
    10621 agtaggacat gtatttggcg gtttgacact gctagatact gtccaatctg aaagtggcat
    10681 ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa gccgttttga acaatattca
    10741 attcatggaa tccgttcatg ccaaaagtta ttcttctatt tttagtacac tgaatacgaa
    10801 aaaagaaatc gaagaaattt ttgactggac aaatacgaat ccttatctgc aaaaaaaagc
    10861 tgagcgtatc aatgaaatct acaaacacgg tacaccgcta gaaaagaaaa ttgccagcgt
    10921 ctttttagaa acattcttgt tctattcagg tttttatact cctctttatt atttaggaaa
    10981 caacaaattg gctaacgtag cagagatcat caaactaatc atccgagatg aatctgtaca
    11041 tggaacctac atcggttaca aattccaatt aggattcaac gagttacctg aagcagaaca
    11101 agaaacgtta aaagactgga tgtacaatct tctttatgag ctttacgaaa acgaagaacg
    11161 ttatacagaa gaattatacg atccaatcgg ctggacagaa gaagtcaaga cattcctacg
    11221 ctataatgcc aacaaagcat tgatgaacct agggatggat ccactctttc cagatactgc
    11281 caatgatgtc aacccaatcg taatgaacgg gatttcaaca ggcacaagca accatgactt
    11341 cttctcacaa gtcggaaacg gctatttgtt aggtcatgta gaagcgatga aagacgacga
    11401 ttatttgatt ggattggaat aaggatttat tttaattcaa aaatttttcg aaaaataaaa
    11461 agagtttagc gtacagatat tttaaatgta cactgaactc ttttttatgc ttattcactt
    11521 gtttcttcca gcacttttgg actgtctaac ttttccgaaa catgttccat tcttctcaat
    11581 aaatagattt tccggatatt taatattatc gtcttaatca ctgcatagac aggtatgcaa
    11641 atcagcatgc ctaatattcc tgcgacactt cctgctccaa tcaataccaa gatgatcgtc
    11701 aaaggatgga tattcattga tttcccaaat aacaacggct tgatcaaatt tccatctaat
    11761 tgttggatca ctaaaatcga caacgccata tacaaagctt gaaatggaga gataaacaaa
    11821 ccgacaatga ttgctggtac cgctccaata aaagggccga catacgggat aatattcgtg
    11881 attccgcaaa tgaccgcaag caacaagcta taaggctgat cgaatattct cattgccaaa
    11941 aagctcatta caccaataat caaagcatct aagatcgtac tgctgatata ggcagataat
    12001 gtttcgttta attcttttac cgtttggcgt aactctttac gaatcgcttt cggaaagaag
    12061 tgagacaacg catcaataaa tttgtgccca tccttgaaca taaatagcaa aataaatgga
    12121 acggtaaata acaaaataaa aaattgcatc ataaatgaaa tgatttttga gacacttgca
    12181 gataaactga ctaatatgat attgaacaaa ttactaagag ataaatttgc tgatgctaat
    12241 tgttcttcca aattgaaatt tttaaactct tcccgctgcg ccaattcatt taaccatttg
    12301 cttgtttcat ctgcatacat tggcaagctc tgggtcaatt gaattgtctg ttccattaac
    12361 tgaggcacta cattcatgac agttaaaaca actaaaatga ttacgaccaa aaacgtcaat
    12421 aaaaaaccga acactctcgg gatccgatgc ttttcgagaa aaacaacgat tggatcgaac
    12481 atataataga taaaaccagc aacaagcaat ggtaaaaaaa ctgccgaaag cattttcaat
    12541 attggactaa aaatattcgg catttgtaaa ataaagtaca cgccgatgat cgtgagtact
    12601 agctccactg tccagaaaaa taattttgat tgtttaaaac gtgattgcat acatctcctc
    12661 caaggtcctt tgatacttct attctatcat ttaacaaaga gttacgcgct gagattttca
    12721 tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg agtagacgag tacttaaaca
    12781 aaaacacggt ttggagtagt gacaaaaatt atactgcagt ccctttttcc aaataaacgg
    12841 tgttcaaaag cttgcatctt cgtaataagc ccagccaaac gaaaaatata aggaactatt
    12901 tttatttggc tgggcttatt acttgatgta ggacatctat tttacaacct cagatcattt
    12961 attgcgcaat actgactaaa tgcaactttg tgatattttt gtgtacggac aactcctcga
    13021 tcacatcggc ataagtgagc ccttttggaa gatcaatcgt atagatattt ttgtacactc
    13081 ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc gattacgata tttttctctt
    13141 caaaataatc ggtaagaaaa tctttggttt cttttctatg gataaattgg atttctaact
    13201 ttttagtcgt aggtacatgg atcacgcgtt tcactactgt gagtgcaata gcaatcccga
    13261 taaaactgat gatcgcaatg gaatagtaac ccatgccgat ggcgatccct aatccagcaa
    13321 ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga ttgtttcgtg acgatgatcg
    13381 ttcccgcgcc taagaaaccg acaccactca ccacttgagc aatcagcctt gcttcatctg
    13441 aacgaatcgt tccagcaagc tgtggatact ctaaagcatc tcttaatgca ccactagcga
    13501 tctcaacttg gatcaatgca atgatacatg ctcccataca aactaaaata tgcgtacgca
    13561 tacctgctgg acgattttta tattgtcgtt caaaaccgat tgctccgcca aatagcatag
    13621 cgatccctaa acgtaaaata atctctggaa ttgtcaatac catatcattc atctaaacac
    13681 cctccagtcc aatatatcat gtctgaaagg agcagacaaa aaaagtgcaa tttttacctc
    13741 ttacgaaaaa aggactgtga caaaattttt gtcatagtcc tttttatgaa taaacggtgt
    13801 tcaatcagct gaccctcggt attactcaac aaacggcaaa atatggggag ctatttttcc
    13861 tttgtcttct ctaatactca ggtcaaaacg ctgatttcac aacctcttta gcctactatt
    13921 gaaaattaca acttacttgc tgcttcttca tcgatgatca caacaacatt gtcgtgattt
    13981 tgcaatacac ttgctggcaa atgattcgtt acaggtcctt caatcatccc tttgattgca
    14041 tcagctttgc tttcgccaaa tgccatcaag acgatttctt tacttttcat aattgaaccg
    14101 attcccattg aaacagcttt tgttggtaca tcttctgctt tatcgaagaa tcgtttgttt
    14161 gcattgattg ttgattctgt taagtcaact actgaagtcg ttccgtcaaa tgaagcacca
    14221 ggttcattga aaccgatatg tccattttgc ccgattccta agatttggat atcgattgga
    14281 tgtgcatcga tgatttgatc atactttttg catgctgctt ccagatcttc tgcttttcca
    14341 tctggtacaa acgtttcttt gaatggtttt ttatcaaata attggacatc catgaaatga
    14401 cggtagcttt gttcatctga accgcctagt ccaacgtact catctaagtt tacagaagtc
    14461 atttcactga aatccaaatc gcttgaagtc atttctttat ataaagtaac tggtgtgctt
    14521 cctgttgcca atcctaatac ttttgcacct ttgttcatac cttctttgat gatttcaaat
    14581 gctttttttc cgccttcttc agcgtttttt acacgaatga tttccatagt gattgctcct
    14641 ttatattttg gtatagccca attatagcat tctttttttc tttttacaat aattagtata
    14701 gaccaaattg catttttttc attttcttga caataaatag gaatccgtgt taggtttcat
    14761 aagatggttc aatcaaacaa tccatgttgc atgaaaacca agcaagtgat tcactgtgga
    14821 cgctttatgt ctgcagtttt tttatgaaaa aggagttatt atgagtatac tgacattaaa
    14881 caacattacc cagcaatttg gcgacaaaat cctctatgaa ggtgtcgatc tgcaattaaa
    14941 tgctggcgaa catcttgggc tgattggaca aaacggagcc ggtaaaagta cattgattaa
    15001 aatcatcact ggtgagatct taccagatga cggacagatc cattggcaaa aaaatattca
    15061 caaaggttac ctcgatcaat atgtggaagt cgatgaaaca ttaacaatcg aagaatttct
    15121 aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa attgcttttc tttaccaaac
    15181 atatagcgag accttcgatg attccttgct ggaaaaagct gggaaactcc aaacggaatt
    15241 agaccaagga gtgttttatc aaatcgacac cttagttgct gagatgagca gcgggttggg
    15301 gatcgatgtc ttaggtttag attcactgtt gaaaaatcta agtggtggac aacgttcaaa
    15361 ggtgatttta gcaaaactac ttttagaaaa cccagatgtt ctgatcttag atgaaccaac
    15421 caatcactta gatgatcagc atatccaatg gctgacgcag tttcttcagg attttgacgg
    15481 tgcgtatatc gtgatttccc atgaccaaga atttcttgat cagatcacca cccatatcgc
    15541 agacatcgag tttggaaaga ttactaagta tactggccat ctaaaacaag cattgaaaca
    15601 aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat gctcaacaaa aacaaatcga
    15661 aaagaccgaa gcttatattc gaaaatacaa agccggttcc cgatcaacga tggcaaaaag
    15721 ccgacaaaaa caattagata aaatcgaacg gctgacgcct ccagcttcag caagtaaagc
    15781 tgtctttgat tttccattct cgcctattgt cacgacatta gcagtggaaa caactgattt
    15841 agtcatcgga tatgatcgac cgctacttgc acccatcaat ttgaccatcc gctttggtga
    15901 aaatattgcc attcgaggct tcaacgggat tggaaaatct actttattaa aaacactgat
    15961 cggtgagatt gaaaaaataa gtggcgattt ccacttccca gataacacaa aaatcaatta
    16021 cttttcacaa gatctgtact gggaaaatcc attagaaaca ccactacaat atttaagcag
    16081 tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa ctagctaaag cagggttagt
    16141 taatcaatta gcaagtgaac ctctcactac attaagtggt ggggaacaaa ctaaggtgaa
    16201 acttgcacag ttgacaatga accaaggtaa tttactgatt ctcgatgaac cgacgaacca
    16261 tatcgatcaa gaaacgaaag aaagcttaca agacgggata cagaaatttc caggaacagt
    16321 aatcatcgtc tctcatgaac aagaatttta tcaagatctt gtgcaacgag tcattgagat
    16381 tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag gagctgtgac aaaagttttg
    16441 tcacaactcc ttcttctgaa taaatggtgg ccaattagct gaccttcggt attagctcaa
    16501 aaaacggcaa aatatgagga gctattttta tttggctatt cttattactt gatgcagaac
    16561 agcttttcac aacctctttt cgtctatttt ttattttcga gatttctctt tcctttaaaa
    16621 acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa gcccgctaac cgttacacct
    16681 aaccagccat actgctgcca cattgtcgtc cctagcaatg aaccagccgc acctccaata
    16741 aagtacataa acataaatac agtattattt cggttgcttg ctgcttcacc tagcccctgt
    16801 actcgtgttt gattggcgac ttgaccaaat tgattcccga cgtctaataa aataatagct
    16861 aaaagcaaaa tggccaaatg gcttccgcca accaataaag caataaagct tattgtttgc
    16921 ataacaagtc ccatatacac gatttttctt tcagaataag aatctgccag tcgtcccacg
    16981 ataggtgcag caactgctcc agacaaacca aaaatcgcta atatccctgc ttcgaatgtt
    17041 ccccaattgt aggcgggact gctgatataa aaaatcaacg tagaccaaaa gatcgaaaat
    17101 gttccaaaca tgaaaaatcc actgactgcc gcttctctta atagacgttg agaagcaact
    17161 aaatgcggca aacttttaag tgaatcaagg tatgaaacag ctgtactact ttgctggaca
    17221 gaacgcggca ttttccagta aagcagaagt gtaagaattc cgactgcaca taaagcaaat
    17281 aaatagatca tccgccaaga aaaaagactg gcaacaatac cagataccgt ccgagaaagc
    17341 aaaatcccca cgagtaaacc gctaagcaat gtacccatga ccttccctct tgcttttggt
    17401 cccgctagta ccgcaccgta aggaatgatg atttgcggca cgatcgaaag taatcctatt
    17461 aaaaaagaag cgcatgcaaa taacgtaaac gaaggcgcaa aaaaagcggc caacagagag
    17521 acggcagata acgctgccat tcgaatgatc aattttggac gatccaccac gtcccctaaa
    17581 ggaactaaaa acagtaaccc taacgcatag cctaattggg tcaacatcgt caatatcccg
    17641 actgcacttg tgctaacaga taaactagct gctacttttg ttccaatcgg ctggatgtaa
    17701 tacatattgg caacaacaac gccacaagtg attgataata gtaaaataag cgaagttgtt
    17761 aaaggttttt tttcattact cttcattgat ttcccccatt tcctatctta gtttacaact
    17821 tatggaaaag attggaaata gaaaaataca actattcgcc acacttatta tttgtgcttt
    17881 tgttttcctt gttttttctt gcgatcttta taaatgaaga caactaaccc tactagcaca
    17941 gtaacacttg caaccaacgt gactgcagtt ttgttatatc cgtgaaacag agcttctcca
    18001 ccacacgcat acaacacaga cgaagggatg actccaatca cgatcgacaa tatgatttgc
    18061 tgtagaggca atttcatcgc atctgcagca aaattgacca tagaagatgg aataaccggt
    18121 atcatatagc ctaacattac acctattttt ggatgtttca tttgtcggat tgcttgcacc
    18181 caatgattcg tttcggtttt cttatctaaa aattttaagc gatgcataag gaaaattgca
    18241 gtgatgttac caagagcatt acccaaaatg ttgatgatcg cacctgtcag tggtccaaag
    18301 ctcaaaccag caatgactcc aactactgaa gtaggaatac ctggaacagc acacatcacc
    18361 gctgtcaata cgatcaagag tatcgatgca aaagtcccat gcgaacgaat atgtgcgagt
    18421 agctctgcct tactcgctct cggatttaaa aataattgga tgtctgtctg atattctaaa
    18481 tacaaccgat agcccactac cagtatcaga atgattccca acgagatcaa tatcaatcgg
    18541 attattttct ttttcatatt tcctcctgaa aaaaatctat attcattaaa taatagttta
    18601 acataaaaac aaaaaagcac cgaaatccaa cataggatct cagtgctttt aaatagtaaa
    18661 aattatttaa cagttacaga tgcgccaact tcttcaagtt tagctttcaa ttcttcagct
    18721 tcttctttag atacgccttc tttaactggt gcaggagcgc catcaactac agctttagct
    18781 tctttcaagc ctaaaccagt tgcttcacga actgctttga taactttaac tttttggtct
    18841 ccagctgcag ttaattctac agtgaattct gtttgttctt cagcagcagc agcgccacca
    18901 gcagcagcta cagctacagg agcagcagca gatacgccaa attcttcttc aatagcttta
    18961 actaggtcgt taagttctag gatagttgct tcttttaact cagcaacgat gttttcaatg
    19021 ttcaatgcca ttataatttc ctccgtttaa ttgttctttt ttatttaatg ctttatgcgg
    19081 caattaagct gcatctccat cttctttgtc tgccactgct ttgacagcgt aagccacgtt
    19141 gcggactggc gcttgtagta cagatagcaa catagataga agtccttcgc gatttggcaa
    19201 ttttgccaat gctgtgattt cttccaatga agagacttta ccttcgatga taccgccttt
    19261 gatttctaat gcttttgctt ctttagcaaa ttcgtccatg attttcgctg gtgcgactac
    19321 atcttcgtta ctgaatgcaa cagctgtagg tccagtgaat acttcgtcca tgccttcaag
    19381 gccagctttt ttagctgcac gtgaaagaat agaatttttg ataactttca tttctacgtt
    19441 agcatcacga agttgtttac gtaaacgtgt tacttcatca actgttaatc cgcggtagtc
    19501 aacgactact actgatgcag ctgctgtaaa tttttcgctt acttcatcaa cgattgctgc
    19561 tttttttgcg ataattgctt cactcatttt tgagtttcac ctcctgaatt ttgatggaag
    19621 agttttttgc actgaaaagg caagtcactt ttatagttac tttgctcaac cagtattatt
    19681 catcacctat tcacttcgtt catagtgatt ctactcgctt tgctcgaaag gaaactcctt
    19741 tcgctttgct caaccagtat tgttcatcat ctcttcactt cgttcatagt gattcacaat
    19801 aaaaaactct atgccaccgc agacatagag ggactattga atatattcaa taaacgtcct
    19861 cggtaggaaa ttaaggcttg atgccaccta ctgtcttcgg tacaagttaa ttattaacct
    19921 caactagctt agcatgctaa actagttgag tcaagaatta tttttcagtt ggatcaatca
    19981 acattctaat atttaattag aaagaagctt ggtcaacatg gataccaggt ccaaatgttg
    20041 ttgtgatcgt tacatttttc atgtattgac ctttagctgc tgatggttta gcttttagca
    20101 atacatcatg gattgctgca aagttttcaa ctaatttttc atcgtcaaat gaaacttttc
    20161 cgattggtac atggatattt ccagctttgt ccacacggta agttacttta ccagctttca
    20221 cttcgtttac tgcttttgtt acgtccattg taactgtacc agttttaggg tttggcatta
    20281 agccttttgg tcctaatact cgtcctagac gacctacagt agccatcata tcaggtgttg
    20341 caacaactac atcaaagtca aaccatccgt tttggatttt ttgaaccatg tcgtcgtcac
    20401 ctacgaaatc tgcaccagct gcttcagctt cttttgcttt gtctccttta gcaaaaacta
    20461 aaacagtttg tgttttacca gttccgtttg gtaatacaac agcaccacgg atttgttggt
    20521 ctgctttttt agggtctacg ttcaaacggt atgcaacttc aactgttgca tcaaattttg
    20581 caatgtttgt ttcttttgct aaagctactg cttcggcaac aggataaact ttacttgaat
    20641 caactttttt taatgcatct tgcatttttt tgctcttttt agccattttg gttcctcctt
    20701 gattgtggtt ataacggttg tacctcccac gtgttctata tctttcaata tagagccaac
    20761 tgagaagctg ctgtcttagg gtgtatagat tattctacag tgatccccat gcttcgtgca
    20821 gttccttcaa ccatgcgcat tgctgcttca acgttagctg cgtttaggtc ttccattttt
    20881 aattcagcga tttctcttac ttgatcgcta gaaactttcg caactttatt tttgtttggt
    20941 tcgcctgaac ctttttcgat tttagctgct tttttcaata gaacagcagc tggcggtgtt
    21001 tttgtaacga atgtgaatga acggtcttcg tatacagaaa taacaaccgg aatgatcaaa
    21061 cctgcttgat cagctgtacg agcgttgaat tcttttgtga atcccatgat attgataccc
    21121 gcttgaccta gtgcaggacc tactggtgga gctggagttg ctttacctgc aggaatttgc
    21181 aatttaacga ttttttctac tttctttgcc acgagacata cctccttaag tccgtgatgt
    21241 ggttaattgg agatgcatat ttctcctccc acgttttatc tgatctgaaa aaatccagac
    21301 aaataatgtg caaaacaatg cacactggta cagtatagca aatttatttg ttttttcaag
    21361 atttttttct gctattttta cattttgctt gaataaacgc taaatttatt ctaaaaaaac
    21421 aacaattttt cgttctcatt tcttctttta tgttacactt gcgctagcat ggttcaacag
    21481 atgaaagcaa acaataacac ggttttttag acccttgtac aaagattcct acataacaaa
    21541 atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt ctttgatatt attggacctg
    21601 tgatgattgg tccaagtagc tctcacactg ccggagctgc tagaatcggg aaaatcatac
    21661 gtagtatatt tggcgaacag cctgattctg tagatattta tctatatgaa tcatttgcaa
    21721 aaacctatcg ggggcacgga acagatattg cattagttgg cggcctgtta gggatggaac
    21781 ccgatgatga acgtttagct gattctttga aattggcata tgaagccggt atggaagtct
    21841 tgtttgttcc taagagtgaa aaagccgaac atccaaactc tgtaaagatg cttctaaaaa
    21901 aaggaacacg aaagctttca gtcactggca tttcgatcgg cggtggcaat atccagattt
    21961 ctgaattgaa tggattcaaa atttctttaa gcatgggtac tcctaccttg atcgttgtcc
    22021 accaagatgt tccaggtatg attgctaaag tgactagtat actttctgag accaatacga
    22081 atatcggcac tatgacagtg acacgggaat caaaaggtga aaatgccatt atgataattg
    22141 aagtagatga tcctcaagta gaagaaacgg taaaaagact caaacagctg ccaaatatcg
    22201 acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt tttatcgatt gaagaattag
    22261 tagatcaagc aaaagcttat ccaagtgtag cagaattgat gattgcagtt gaaatggaaa
    22321 tgagtggccg ttcccgtagt cacatcatcg agatcatgga aaaaaacctc tcagtaatgg
    22381 aacaatcaat caacgaggga aaagaaggtg ttacctccgt cacaggaatt accggtggtg
    22441 atgcaaaaaa attagatgat tatttaaatg aggcagactt tttaagtgga gaaacaatct
    22501 taaaagctgt acgcaatgct gtagctgtta atgaagtcaa tgcaaaaatg gggttgattt
    22561 gtgctacacc gactgccgga agcgcgggtg ttgttcctgg tgtgctgatg gctgtccgag
    22621 atcggcttga tttgacacat gaacagcaat tggaattctt atttaccgct ggcgctttcg
    22681 ggttagtcat cgcaaacaac gcttctatca gtggtgcaga agggggatgt caagcagaag
    22741 ttggttctgc aagtgcaatg gcagcagcag ctttggtttg tgcaaaaaat gggactcctc
    22801 atcaagcagc acaagcagta gccattacat tgaaaaatat gatgggactg atttgcgatc
    22861 ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa tgctttaggc tcttctcaag
    22921 cttttatctc tgctgatatg gcattagcgg gtatcgaaag tgtcattccg ccggatgaag
    22981 tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc gattttcaaa gaaacagcag
    23041 aaggtggctt agctgttacg ccaactgcta aacgacttgc ggaagagatt ttaaataaga
    23101 gggcgtgata aagctcctta atttcgagta ttaagagatt agttatcttc ataacaaaaa
    23161 acgaagagag gactaattgc ccttctcttc gttttttatt aatccatcaa tttcgtatat
    23221 tttacttcat cgatcgtttt tgttcctgca agttgcatcg tgatcgacaa ttctttgttc
    23281 aggtgttcaa aaacagatgt tactccttct gccccaccta gattcaaccc ataaatcact
    23341 ggtcggccaa tcgcaactaa gtcagcacca cttgccagag ctttgaagac atgctctcct
    23401 cgacggacac cagagtcgaa aatcactggt acacgtttgt tgactacttc ggcaattttt
    23461 ggcaatactt caaaggacgc aggacctcca tctagttgac gtccaccatg attggatacc
    23521 cagataccat ctgcccctgc agagatagcg attactgcat cttctggcga ttgtattcct
    23581 tttacgataa ctggaagatg ggtgatttct ttgattgttt tgatgtcatc tggtgtcaag
    23641 ccttgttttg cagctgcata aatttctgcg attccttttc cttcgccatt accacttgca
    23701 ctttgttcgc tatacgcagc taagttaggc attggtaatg gaaattggaa ttgattgatg
    23761 acgtcttctt cacggtaacc accgagcgtt gaatctgccg ttaaaatgat agctttagcc
    23821 cctgcttcta ctgctttgtt caaaatgaac tcattgaatc tgtcgtcttt actcatatat
    23881 aattggaaaa actgcggtgc gcctggcgct gcatcggctg cgtctttgat tgtcgtattt
    23941 gcataagtgc tgatggaaaa gattgatcca gcagcagcta ctcctttagc tgtatcagct
    24001 tccccttttt catgtgccaa tccttgggcc gctgatggcg cttggataat cggtgtatct
    24061 aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt cgattccgcg taatatccgt
    24121 ggcatgattt tctttgtatt aaaagaagtc gtattttctt tcatcgtcca ctcatcttca
    24181 gaaccaccgc gaatatagcc aaaagctcct gcttccatgc gctcttttac acgaccttct
    24241 agttaggcaa tgtttacaat atcgattgga tgttcttcat tacttgtatg ataagtcata
    24301 gtagatcctt ctttcatctt cttgattaca tataaatcat actaaactta caaaaagtaa
    24361 gcaaacattt actctcagat acttttatcg ctattctttc caaataaatc gacttacaag
    24421 ctgatctact tcttggtttt catgaagcat actatgagag gcattttcac ctgtcactac
    24481 tttttcctct atttcatttc cgcgttgctt caacaacgca tagaccgcta atgcactgtt
    24541 taacggtact gttccatcac tcaagtctgt ttcatctagt tgaccagcaa tcaagaaaaa
    24601 tcgagtggtt ataggaatat taccgatcat ttgctggtaa tcttgatagc ggttagaaac
    24661 gacagcaggc ccctttgcta actcatctgt cagagactgc gcactatcat cgacaaaatc
    24721 attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt ggcaatgacg tatcctgtcc
    24781 atacgttcct aaatagcgta gtgcactaac tcctcccatg gaatgcccca ctatgttgac
    24841 tttatctatg ccgtagtttt ctttcagata caaaagacaa gttttgatcc attctgcttg
    24901 attccactcg ttatttttat tatcttcaaa caatacttgg accatcggat tgtctgcttt
    24961 tccagacaaa tctcctgttg cttgtatttg tccatctgtt cctactgtta aaaccaattc
    25021 ttttactgct tgctgatttt tttccaaacg ccgcaacatc cctttaaagg aattttctgt
    25081 cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt ttttctatca ctttactgga
    25141 ttctgccgtt ttttctttat cagaaacttc agtagtattt atcttcgtcg tctggcaacc
    25201 acttaacgca aacaaactga taaggactaa agctgctgca gttgcacgtt tgcataaatt
    25261 tttgattggt tctgtcatac acctcttcct ttccatatag aaaaaagaaa gacttgaaag
    25321 gccaatcata tgatcgacat ttcaagtctc tttattaatc gattttatct acttgatcga
    25381 aatcaagttc tgtactggtt tcacgtccaa acatatcgat gttgactttc aatttttctt
    25441 tttctccgtc aacttctgtt acttgtcctt ctaatccagc aaatgcgcct tcgatgattt
    25501 tcacgacttc gccaacttct acttctagat cattttgtcg caggctcata ccgatagaac
    25561 gcaagatatg attgacttct tctggaagta atggtgctgg tttgcttccg gctccgtgtg
    25621 aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac ataccatgaa gcgtctgtca
    25681 tgatcatttc aacaagtacg tatcctggga atgttttatg aacgatttct ttttctttcc
    25741 catttttgac ttcagtttct ttttcttcag gaacaacgac acggaaaata aagtcggcca
    25801 ttttcatact ttgcgcacga gattcgatat ttgcttttac tttgttttca tatcctgaat
    25861 atgtatgcaa gacataccag tttttttcaa aagattccat tgaaggctcc tcatttcatt
    25921 gtaaaataaa aaaacctcag tacacccgaa gtttttcttt cgcttcatta tagcactaaa
    25981 agtacgttgt ggctagcgag ttattttaaa atccagccaa ataagctttg gatggctgta
    26041 tccattacgt aaaacatcaa tccgaaaata atagaagttt cgatgactac taacgtatct
    26101 ttacggagtt gttttctgct tggccaagtt acttgtttga tttcatcttt tacgcttttt
    26161 agaaatttca tgagacaacc tcccttgcta ctgtttattt cgtttcttta tgtaatgtat
    26221 attgattaca atatttacaa aacttattga tttccaaacg ttcgccgctt tttccttcac
    26281 tgactgattt tgtgtagttg cgagacccac aaacggaaca agcgagtgct gcttttttct
    26341 ttgccattta cttctgcccc cttgctgaga atcagactta tacttcataa cagtatcacg
    26401 taacttttta cttgtcaatt tgtgaaataa actatctatt gttttggata aaattatgat
    26461 atgattaatt tgaaattaag ctaaaatgag acaattggag gtatagcgat gctattaaaa
    26521 acacttgtat ataagaaaaa agatctgaca accgttacag agactagtac attagaagaa
    26581 gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc cgatcttaga ttcttcaggt
    26641 aatattttcc gtggaaacat ttacaaaatg catatctatc gtcataaagc aaatggcggt
    26701 gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta caaagttcat ttacctggat
    26761 acttctttct tcaaagtatt ctttacgatc aaagagcttc cttatattgc tgtccttgac
    26821 agtgaaaatc atttttatgg tattttgaca catagtacgt tgcttaatat gctctctcaa
    26881 tcttggagcg tagaacaagg aagctatgta ctgacaattg cttccacagg aaagcaagga
    26941 gatttggctg ctattgcaaa aatcatttca aaacacagca gcattgccag ctgtattact
    27001 cttgatatcg aaaaagacga atacattcga cgtacattga tcacgctacc ggctaacact
    27061 gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga aaaagaatta taaagtggta
    27121 gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa gcgaaaaaag aaaacagcta
    27181 atctgctttc ttttttcgct ttttataatt agacacgctt ttcaaaatca agcgcgcgat
    27241 ctaaataata catcaatggt gctgaaacag ccatgacgat aaactcactt aatgcaagcg
    27301 tgccataggt tgtccaaaat gcttggccac ctgttggaac taacatataa gcaatcagtc
    27361 ccatgctgat ggtaaagaag atcgtgttca atactaaacg catctttaca tttttcactt
    27421 tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt ttggccgcca ccaaaaacca
    27481 catccatgat accaaaacca aagaaggcat tatagacgat gaccccgcct aaaactcccc
    27541 aaagcaattt acggttaaag acaactaaat gattcaaact ttctgaaata cgaaactgga
    27601 tcggccctga ggatacaggc gcaactaaaa tagttaacgc tagataaaga gccatgatga
    27661 tgccatttac aacgattacg gataaccgtt gtctgctttt cgttttttgc attttctctc
    27721 ctcttttctc ctagtttttt taacgcggga tggttgatga accgcggtta tgtgaaacaa
    27781 acaataatta taacagactt ttcatttatt tcaatccgtc ttatctgctt ttaaaaaagc
    27841 aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg atatggtata aagtaagtgt
    27901 aaggggtcat aagaattccc agtaaagcag acgaagtagg atctgcaaga acagaattgt
    27961 tgtgaaactt aaataaataa aaaaggatgt gggaatttga gcaacctctt actagagaac
    28021 ggtctagcaa gcagctgaag atctaagtgg acatgccgaa ccttggaact tctccttacg
    28081 taagtaatgg atcatgcgct tgctaggctt ttttgtgttt ttcggtcttc ggaccttttt
    28141 cattttccta gacttctgct ataataacca agaattaatt ttgtaacaga tttttttgaa
    28201 aaaacaagtt tctttcattc aaaaaataga aaaagcttgc taattattgg ttaaccagca
    28261 aacaggagta ttaaaatatc ttgttttatt gaatagcagg aggaaatcat gaaattccat
    28321 tatcatatat tgatcaatcc atcggcaggc agcggaaatg gctataaagt agcagaaaga
    28381 attttaccgg tattaaaaaa taagcatatt gactatacac ttcattatag tgaatacaaa
    28441 ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa cattgattcc ttggatagaa
    28501 gaagaacaga aaacaatcga tactttcccc cttttgatca tcgtaggagg tgacggcacc
    28561 ttacatcagg tacttgatac tttctatcag atggaagtag aattcccagt tgcttatatt
    28621 ccagcaggtt ctggagatga ttttgctcga ggggcaggcc ttccaaaaaa tcccaaaaaa
    28681 ggacttcagc tgattttagc agctcaatca ccagaaaaag tccacataat ggcttatgaa
    28741 gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt tcggtatagg attagatgca
    28801 gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc gattgaacaa atacaactta
    28861 ggatcatttt cgtatttatt ctctattctc cgtgctttat tcactcaaaa aggatttcca
    28921 attctggtag atgcaggagg aaaacaatta agtttttcaa acgcattttt atgtacagca
    28981 acgaagcatc ctttttttgg cggaggaatc gcgattgttc caactgctga tgcgtctaaa
    29041 ccagtcattg attttgtcgt ggtcgaacga atcaatttct ttaaaatctt gtggctcatt
    29101 cttttactta tacagaaaaa acaattgaaa tcgaaatatt tccatcacta cacaacaaac
    29161 agattacgta tcgtttcaac aacaccgcaa catggtcaag aagatggaga agaaatggaa
    29221 aaacgacctt ttgatatcac aataaccaca actaaacagt tgttttggtg ttaataaaag
    29281 aggttttgtc agaagctaca tctggaacac cgtttattcg gaaaaaagga ctgtgacacg
    29341 acttttgtca cagtcctttt tttactatgc agttaattct ttgcccaatt gggttgccat
    29401 cgatagtttc tttctttacc caaaataaat tggatcattg aaaacaccgc agcaaaagca
    29461 actaagacac aaccattgaa ccaaaaagat tctgcaaatg tttgattgta actgagtgtt
    29521 tcactgatca agttattatt ttgaacaaac caatatgttg gtgtgaatgc cgctattttg
    29581 ctaaccattt ctggcaagta ttctgctggt acaaacaccc caccgataaa gcaactgcct
    29641 gtgataaaaa tatctttgat cccaccgctg gtttctgagt tttttaccaa tgaagtgata
    29701 caagccgaaa agctaatgat cggcaataaa aacaagaaag tatttaaaat atgataccat
    29761 accgttatat cccacgaggt gtggatcact aacagaacca ttagaagtaa gaagccactg
    29821 aacaacagca gcgagtaact aatcaaacta aaggttactt tgcgattcaa gtttcgtttg
    29881 gtaatgggcg cactctggtt tcgtttactg atctctttgc gattaaatgc tagattgatg
    29941 acgctgaaac cgctaaaaat ggatagaaac aacccataag acaagaaatt aaaggttgtg
    30001 cccgtcaaca gctttttcat ccgctgacta tatcccgcac ttagctcaac ttctccagta
    30061 accgagagtg ccatttgtgt ctgttccata gctgcatcga ttgttttatc ttcttcttga
    30121 aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg taacaactgt tttagaaaag
    30181 gcatctgggc gactctgtat cttgatcgtc ggcatctttc ctacttcgac ttgttcggta
    30241 aagtctgcag gcagatgtaa gataatgttt acacgattaa aataaagagc atcatcgatt
    30301 tctttttgtg aggttccatc caatgtaacg actgtttcct tgtcattcaa atatttacga
    30361 aggccatcga ctacaggcga ctcttcttca ctaatcaagg cgattttcgc atcttgcaga
    30421 gaggtatttt caacatcaat attttgcgca aaaaagaatg tggcaacaac agtgataatt
    30481 acacctatca gtaaattcga tttatttgct ttaagcaatg tccaaatcgt tttataaagc
    30541 gtcataacgc acccctcttt ccatccaagc atttcctaac gcaaaaagga aaataaatcc
    30601 gatcaaccat agaagattgg tataaaaact agccaaatca gtgtagaaat aaagtttata
    30661 caaactttcg ctgatcaagt tgacgagatt taattgacca acaatcggaa tatgaagatc
    30721 aatccaatac ttcacttgtt ccgaccccat cataccagca aaaaagctca tcgcaatcgt
    30781 cactgaaatc aagatgctat ccttttgcac cagattcatt tttggtatca aattaccaac
    30841 caatgtacct aacaaaatcg cacacaacga ccccagagcg gtaacaatca tcaaccattg
    30901 ccaacgttca ccaaagtcta cttgatagat aaaacgaaag acagctaaca taatataaat
    30961 ctctgtcaaa acaatcgtaa agcttgctgc caaattagcg attgaaacta acgattgctt
    31021 tctaggaatc aaggacaagc gaataccatt tgacgattga tttgcttgct gatcctgtac
    31081 attgcggacc ccccacatcg tcccgtagag aatcgccatt ccaatcagag tgaagaaata
    31141 aaaacttttg aaactcatat tacgacctgc atgaacttct tgaaaagata cttcttgttc
    31201 aaaaacagtc atgatgggcg cgagatcaac gttctccact tctgccactt tactcgaaat
    31261 tataccaata cgctgtaaaa attgatccat gacgtttttc atcaacgtct gactcattcc
    31321 tgcttgaccc acatggagcg aaacagatga accatcaatg agataaaaac cagccaaatc
    31381 atctgcattt agcttttcat tcgcttctac ttccgatagt tcctgtactt caaataatgg
    31441 tttatcattg acttcgactt gttctaagac tgtggtaaaa ttttccgaaa gcgctgtttg
    31501 atccgctttg attacaattc cagtattaat cgtttcgaga ttgccacctt gatcaatctc
    31561 gccaaaggcc gccataaaca tcaatcctaa aagaattgga aaggccaatg tccaaaacaa
    31621 catggattta tttcgcagca gtatttttgt gcgatattta taaaaatgcc aaaacattat
    31681 ttcccctcct ctagtctctc aaatccttgc cagtaatctc taaaaagaca tcattcaagg
    31741 acgcttcttt ggttgctatt tttccgtaac tgatcttttc ttcttgtaag attgtaagaa
    31801 tcggcaacaa tactgtattt agttcagtag ttgttacttg gagctgacct tttttataag
    31861 cgattccatc agaagctatt gttcgctgac aacacgtttt ctgggagtaa tgggatgtcg
    31921 atcgtcaaac gatccgccgt ccgtaccata tcttttagag attctttggt tcctttagca
    31981 atcacctgtc cttgatccat gatcacgatc tggtcgcaaa gaatttctac ttcttccatg
    32041 taatgtgttg tatagacaat ggttgctcct tgtcgattca gctccttgat actttctaaa
    32101 attttattcc gactttgggg gtctacagct actgtcggct catctaaaaa aattaaccgc
    32161 ggcttgtggg caatcccaca tgcgatgttc aaccgacgtt tcaagccacc actaagttct
    32221 cctggacgaa acgaaacata tttttctaat cccactaatt gaatcacctc ttcaatcaag
    32281 gctttccttt tctctttttc ggaaacatat aaaccgcaaa aataatcgat attttcttgc
    32341 acacttagtt cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat tgccttgaca
    32401 gcataatttt caggagtcat tgttgtcccg aacaatttaa tggttccttt gtcataattt
    32461 aacaaagaaa gcatacagtt gatggcagtc gatttcccac tgccgtttgg tcctaacaga
    32521 cctaagactt ctccttcttg tacttgcaga tcaaaatgat tcaatgctac aaaatctcca
    32581 tagcgtttga ctaatttatc aatttcgata atcatcatga aaaactcctt ccaaatttct
    32641 tgttgatctg actataataa agtgcaagaa aagaaactag tgacgattgg ctaaaccaca
    32701 catgacattt gtcacaatga aggagtctgc tatgcataaa tctcggctac tcaacgaaac
    32761 tagctttcta ctggttatct cattgctttt gctggtaagt caaggattcg ttgctatgca
    32821 agttatctat ttattgatcg ccgttttatt cactggtgtc agttatttac tgcctacaaa
    32881 gccaaattgg attttccaag ttattctatt tcttttatgt gcctttttac ctgatcttgt
    32941 cctgtttctg ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc ttgattactt
    33001 gtttttgtta ggttttttgt tgatttcgac ggttggtaat catcccccca tcaaggtcgc
    33061 ctttgtcgta attttgctgt ttttttcctt ttacttacga aaaaaagatc aacgaacgac
    33121 cgatcttgag aaaaagtaca ttgtctccaa agatactcat tgggaaaaac aacaagagtt
    33181 atcggaacaa aataaagcat tggccctgtc acaagaaaca caattagcct tgcaaattgt
    33241 ggaagaacgg aaccgaattg cccgagacat ccatgacaat gtcggtcatc tgctgtcaag
    33301 tgcgatttta caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac taaaagaacc
    33361 attagaaaat ttaagtgcca ccgttcacac tgggatggat cggattcgtc aaagtgtcca
    33421 tgacctccat gagacatcga tctcttttca acactcctta gagtttctga tcgctgattt
    33481 tccttcttct gttacgattg aaggaaactt gtttgaagga ttgaatgaaa cgcagcaaaa
    33541 ctgcttctta atggtcatca aagaagcctt aaccaacagt atcaaacata gtcatgccaa
    33601 aaccgttgtt ttacattttc gaacgttgcc ggccttttat cgcttgcaaa tcacgaatga
    33661 tggcaccagc ccacaaaaaa attctgctcc cggtattggt ttgaaaacga tgcgtcaacg
    33721 tgttcaagaa caaaagggac aagtccatat tttccaagaa aaccagcaat ttcagttaac
    33781 gatcatttta ccaagggagg gacaccaatg ataacagtag tcattattga gatgatccat
    33841 ttgtgacgaa atcgttacaa accattttag aaagcacgaa agagatccgt gtgttaggca
    33901 ttggtcattg tgccaaggat gcgctagtct tatacgaaac ccatcgtccc gatgttttgc
    33961 tgacggacat ccgcatgcct aaacaaacgg gcatcgatgc tgccaaagaa attttggcta
    34021 aatttcccaa agccatcctt ttgcttttga cgacattcaa agatgaagaa tacatccgcg
    34081 aagcattttc catcggtgtc aaagggtatt taatcaaaca gaacttgcaa gcgattattc
    34141 cttctgtcaa atccgcttat aatggacagg ccgtatttgg taatgaaatc atcgagactt
    34201 tcactcaaat gatgaagaat aaaccgacga ttgatagtca tgcttatgct tcttttagtg
    34261 aacgtgagct tgcaatcatc aaagaagtag ctgccgggaa aaacaataaa gaaattgcag
    34321 atgccttgta cttgagtgac ggcactgttc gcaattacat cagccaatta ctagaaaagc
    34381 tagatttacg ggatcgcacg caattggcta tctattttta tcagcatttg caataagtct
    34441 tatctaacac aaaaaaacct gaatgatgct ccttcgcatc attcaggttt ttttcacatc
    34501 ttctattcaa ttgtcccgca aaccatttcc tcacgattga aagaatcaag gattcgcagg
    34561 gctgccagga ttcgttggat tgttcggcgt gttaggcgga aaaataggcg ccttgtctaa
    34621 cgttttttct agttttgatt cttcacgatc gtctcgttct acatcaggac tttcattcaa
    34681 tttgaattct ttttccatct catctaactt tttaaagtca ttgccagatt cttttgctaa
    34741 tttcgctaat ttatcttcgg tttctttagc cataaagtaa cgcccccctt ttgatttcag
    34801 cttagcatat cacactgaaa aaacaaaaga aaacgctaac caacgatcat ttttagctat
    34861 ttctcatctt taaaatcaag cattttttgc cggtataaaa tcgtagggag aactgttttc
    34921 cataccaatc atcggatcta ctaactggtt atcgatcact tctgctgtaa gaacgaaatc
    34981 tttagaaaca gcaggttcta cctcttcttt cttttcttct ccaacaaatg aagaaaacag
    35041 atctggttcc actgtttcca ttacttggtt tttcgtttca ttcgtcccac tgtcatcttc
    35101 tacaggtagc ttttctttta caacccgtgg ctcttttgta tagttcccat cttcaaatgg
    35161 atcatttccg accaaagaat cttcataagc tgctagtttg gtccggagag tgatattcat
    35221 tttatcatta tccatgatcc ttgatttcaa cgtagtcatc cggcttgctg attcattcgc
    35281 tacacaagtg acataggctt gctcttcccc taacaaaatc aacagttcct tactgcgagt
    35341 cactgccgtg tagagcaaat tacgctgaag catccgctga tattgatgga ccatgggcaa
    35401 aataaccatc ttgaattcac tgccttgtga tttatggatt gagcaacaat aagaaagagt
    35461 gattttattc cattcgtttc ttttataagt gacctcattt gcatcaaact ggataactaa
    35521 ttcatccact ttatcttcag aatcttttgc caagatgata cccgtgattt ctcccatatc
    35581 tccattgaat acattcaatt cgggagtatt gactaattgg aggactttgt ctcctatccg
    35641 ataaacggtg tcgttccaat gtacttcctt ttttccaggt ttccctggat tgaatatctc
    35701 ctgcatcatt ttatttaacg catcgatccc tgcaggtccc cgatacattg gtgctaatac
    35761 ttggatatct tgtgcacgga atcccttatt tttcgctttt tccacaattt tagagatcaa
    35821 gggttcaatt tgataagcat cacttcggaa ataggaacga tctttttgat tctttgtgaa
    35881 atcttttggc agacgtcctt ctttgatctc atgggcaaga ggaataatac tagaaccatc
    35941 accttgccga taaatctgat tgagttccat tttgggaatt tgttcgattt ccaaaagatc
    36001 atggagtact tgaccaggac cgacagaggg aagctgatct ttgtctccta ctaagataac
    36061 ttgcatattc gtcggaatcg ctttgaataa tgtgttagcc agccatgtat cgaccataga
    36121 catctcatca acaatcaaaa gtccgccttc taattctttc gtactgactg aaggtgtttt
    36181 ttcccgtcca ttcagcccca gaagacgatg gatcgtactg gcaggcagac ctgtcgtttc
    36241 gttcattcgt ttagctgctc ggccagttgg tgctgcaagt agaataggaa aaatttcttg
    36301 ggtatatttt gaaggatcca attccagtcc gttcaactct gcaaaaagat tgacgattcc
    36361 gttgatcaca gtagttttcc ccgtacctgg tcctcctgtt aaaataaata gaggggaacg
    36421 gattgcttca gcgatagctt gttcttgcga aggtccatac tgtatgccga atattttttc
    36481 gatacgccga atattcttct gcacttcttt ttcaggataa tggatttctt ttttacgact
    36541 gagaaggcgc tggatcgaat taccgatccc ccattcagaa aagtacaaac tgttttcgta
    36601 aagattagtc tcttcttgtt gtatctttcc ttcttcaaca agttggataa ttacagtagc
    36661 aacctgatca ggctggattt caattgggcg acttgtttct aaaatacgga tcgtttgctg
    36721 aagcaatgtc tccgccggta cgtatgtatc tcctgtttga actgcatgct gaaaaatctg
    36781 atgcaagata gctgctcgaa ttcttctcgg tgaatctgct gcaataccga tttgttcggc
    36841 tagattatct gcacgtttga acccgattcc ttctatgtct tcgactagct ggtacggatt
    36901 ttcttcgatg atatccaaag actcgttctt ataggcctgg tagatactga aagctaactg
    36961 actaccgaac ccgtaacgat tcagtcccat gatcacttga tccatcccgt ggtttagccg
    37021 aatcgtctca gcaagcattt ctctttttgg cttcgtcaaa ccggagattt gttttaatac
    37081 cgaaggatca gcaatgattt tttcgatagc atcttctccc agcaattcta caattttttc
    37141 tgctgttttc ttcccgattc ctgggaattt ttcgctggaa agatacgcta cgatcccatt
    37201 tgcagttgtt ggcttttctt gctcatatct ttcgagttta agctgccttc cgtatttagg
    37261 atgatccact aattctccat aaaaacgata agtctcattt tcttgaatat cgccgaaact
    37321 tccggtgact acaatctctt tttcacggta atcagctgtg gtttcactga tgtgtaccaa
    37381 aagcactttg taaaaattac taggattttg aaaaaaaatc gcggcaactg taccaacgaa
    37441 ataaggcatt tcttctggct gcatcggaag ccgcctcctt cttctatagt aaaggttctg
    37501 gtgctgtttc agttcctaaa aaatctgttt ggttccatac tttcagttca tatggcaata
    37561 atctgcaagg ctcttcagtc tctaaaatcg tcaaactact attgaacaat ccacccattt
    37621 cacgcaactc acttagctct tttcctgtca tccattgaat ggctgcagtt aaagaagctc
    37681 catgtcctac gaataaaagt gggccatctt tatgttgggc aaccgcatct gctacaacag
    37741 tttcgatacg tgtgattgcc gattcaatcg tttctccgtg aaaaggagaa ggatcataca
    37801 gatctaaatg attacgcaaa ttcggcagat tatccggata tttctggtac atttcatcga
    37861 tcgactgacc ttctaaagta cccagcccta gttctcttag cttatctgta taaaaaatct
    37921 ctaccggacg agcaagctct ttgttgatac cttctgctgt atcacgtgct cgctttgctg
    37981 tactggaata aattgcttta aaaggaacat attttacctt tttcccaaat gctttgattt
    38041 cttcgtaact ttgtggcaaa agcggagaat cgccattgct cccttgaaaa cgtctttcct
    38101 gattccattg tgtcttccca tgtcgggtaa aatatagttc catacgcagc aaactcctgt
    38161 ctttctttta ttttactcta taataaactg tctaatccag cttgtcattt tcatagtgat
    38221 gcgtcaattc taaattaggg aatccttgct ggcgaagtgc ttcatacact atcattgcag
    38281 ctgtattcga taaattcaat gagcggacat gctcatcatt catagggatg cgtaaacact
    38341 tttcttcgtt ttcacgcata aatgtttctg gcaaaccggt tgtttctttt ccaaataaaa
    38401 agaagtgctc ttctccatca gcaaaatttt cgtcactata tactttatgg gcaaattttg
    38461 agatcaaatg tagtttacga ttacctaaaa atgttaaaaa agcttctaaa ttactgtggt
    38521 aagtaatatt tacatcattc caataatcca accctgcccg ttttagatgt ttgtcatcag
    38581 ttgaaaaacc tagtggctcg attaaatgaa gcggagtatt cgtggctgca cacgtacggg
    38641 caatatttcc agtgtttgca gggattaatg gttcaaataa aacgatatga ttcatcagga
    38701 tcattcctct catatttatg ttcgtttcta ttttctgaca atttgatttt tgcataaaaa
    38761 aaaacgtatc aactcggtgt cagtcactgc gagtcgttac gttagtgaag ctcttctcac
    38821 taacttttat cagaacagga acctgataaa aaccaatgaa aaaacaactt gttgctaata
    38881 taacaccctc aacttttttt tgcaacctct actcattaaa aactgctaga aaaacgtttt
    38941 ctcttagttc cttttaataa ttctatgtca attgtaatct tcttcaaagg attcttatat
    39001 aagtctgctt tgattcgctc ttctacctgc cattctaaag gtgacatggc caaaagatct
    39061 ttttgatttt cctctggtat ctcaaattca taggttacac gctgacgttc agttaatttc
    39121 attgcttcag caaacttggc tactacacgt tcattggaat aactctgttt ttcaggttga
    39181 ttcggataaa aagcctgacg tagttccttt aaataatttt cttgtggcac caccttcaac
    39241 acttctccat cttctgagag tacacgttgg aattcttggt aatgagaagg cgaaaagata
    39301 tttaaaaccg cgttgaatgt ttgatcagca aacggcaaat tggtcaaatc agctacacac
    39361 caaaatgctt cggcagattg ttctgtagct gcgtagactc cttcttttga aatatcaaat
    39421 ccaacagcat tttgaatctt tttcaatttt gaaagttcat tcaagaagct accttctccg
    39481 cagccaacat ctaatagcga gacttcttca ggtaaatgct gcgcaatagt ctcaataacg
    39541 ggctgataca tgccagattg gatcattcta cgccttggaa cgaacattcc ttgatcgtat
    39601 tctgttttaa ccgcatggga taaaaaataa agtgttcctt ttttggaaag gtcaaaacgg
    39661 tgattatttt tgcatatcaa gccattatca gactttttta cttcttcttg gcaaaccgga
    39721 cagcggaaca gctgatgatt ttctgccaaa aactggcgtc ctcggtcaat tttttttaac
    39781 atagttcccc ctcatttctg ttttatctta ccgttagatg ctcactttgg caactgctaa
    39841 aaatatgata caatgcctag aagaaagtga aagataaagg agaagattag atgatcaaag
    39901 aattttgcgc tgaaaactac acatctattc ctctagcaat tgctagagga gccgatcgga
    39961 tcgaattgtg tgacaactta gcggtcggtg gcacaacccc gagtactggt gtgatcgaag
    40021 aaacgttaag ttatgctggg gaaaaagata tccccgtaat gacgattatt cgtcctagag
    40081 ggggcgactt cgtctataac gatatcgaat tgaaaatcat gcatactgat ttgattgaag
    40141 caaagaaatt agggacggat ggcgtagtat ttggttgtct tactccttca ggctggctag
    40201 acgaagaggc cttagaacta ttgatcgaaa cagcagaagg gttgcagatc accttccata
    40261 tggcttttga cagtattcct gaagatcggc aattcgaagc aatcgattgg ctggtagaac
    40321 atggtgttga ccgtatcttg actcatggcg gtcctgctga caaaactatt gtagaaaatt
    40381 tcccacggct taaagaattg attgcctata cagatggccg cttaatcatt ttgcctggtg
    40441 gtggtgtcac tgcagagaat gcagcacatg tagctaaaga attgaacgta agcgaagtac
    40501 acggaaccaa aatcgtggat cttcagttat aatcttataa acaaaaaaac atttgcaaca
    40561 ggcataatgc ttattgcaaa tgtttttgtt tttttacata aaatagccca taaataaaga
    40621 agcaatattg aaataaatca gtacactcgt caaatcgctc aaagtcgtga taaagggacc
    40681 gcttgctact gctggatcaa atcctagacg atccattagc atcgggatta aactacctgc
    40741 aagattcgcc actgtgattg caaatagcat agccatccct atgacaaacc ccaagataaa
    40801 attatgtttc caaaaaccta cgattagaaa aatcgtgatc cccgtaacag ctcctgtgac
    40861 aacgccggtc aagacttcag ccaaaacaag tcgaacaaat ccatgatctt tctcatcaga
    40921 aatcgcaagt cgacgaacag ccacagctag cgactgggtt ccagcattac cggcagttcc
    40981 tgtaatcagc gaaatgaaga ctgctaaaat actcgcttca cttaccaagt cttcatagtg
    41041 actgattaaa gaagcggtcg acatacctaa aaacaacaga gtgatcagcc aaggcaaacg
    41101 tcgagacgct gcctttactg gattttcatt aatttcttcc acattgaccc cggcaagtcc
    41161 tgaataatcg ctagcagctt catcatcaat aacgtcgatg atatcatcga ctgtgacgat
    41221 tcctaacaaa tgatcatcat aatcggtaac aggaagagct aagaagtcat aatctctaaa
    41281 tgtttgtgcc acatcttctt ggtcgtcgcc tacatgaact gaaagcactc gttcgctcat
    41341 cagatcagaa atcattgcgt cgtcatcatt cacgattagg tcgcgcagag aaataacccc
    41401 tactagctga ccttcttgat taacgacata gatgtaatag attgtttcag ctacatctgc
    41461 ttcgttcttc agcacgtaca tcgcagagcg aacagtttga ttggctacga tcgaaacaaa
    41521 ttcagttgtc atgatcgcgc cagccgtttc atcctcgtaa tggagaagtt ccttgatttc
    41581 actggcatcg tctgccgaca tcaaacttaa atatttagca atttgctttt tatccagcgt
    41641 attaagcaag tcaacagcat tatctgtata catctcagaa agcatatcag ctgcatagct
    41701 aggacgcatt tcatctaggt attctttcat atgttcgttg tcttcttcaa tcacatcgaa
    41761 catatccgct aattctttag gcgacagata ggaatagatc agctgcctgt tctcttctgt
    41821 aagagattgg tagaattgcc cctgctcata gatatgcatc tctaaaaaac tgtttctgaa
    41881 tgcctgaata ttattttccc ttaaatcttg agtcaactgg gcaaatcttt cctctaattc
    41941 ttgattttca tccaaaccaa tctcacctcg ccttattctt caaaccgtct cacaactgcc
    42001 tccatgtctg cggctaaagg ctgcttgaac tccagcaact cttctgtaaa tggatggtaa
    42061 aaacgcagat cataacaatg aagggcttgt ctttttattc ccagatccat tcgcccgcca
    42121 tacatatcat caccgagcag ggcacagcca attgcggaaa aatgaactct tatttgatgt
    42181 gtccttcctg tatgcagctg gatatcaact aacgcgattt ctgttagtcg cttctctatc
    42241 cagtactcag tctgtgccga acgtccttca tcagagacca ttcgcttcaa tagcgaagaa
    42301 taatctcggg cgatcggcaa atcgatatgt ccatgtgccg ataaatctga aagccttcca
    42361 ctgactaagg cctgatattt tttcacaaac ttcttttcac gaagttgcat atctaatttt
    42421 gcgtgcgcaa atccatgctt tgcaaaaagc atcaaaccag atgtatcccg atctagtcgc
    42481 gtcaccacat ggattacttg gttttcgtaa ccttgttttt tataataagc ctttacacga
    42541 ttagccattg tcccatttgg atggtattgg gcaggaatag acgaaacgcc tgctggttta
    42601 tttactacca gaacgtgttc atcttcatac acgatatcga tcggtgtttc atccaaaagt
    42661 acggtttcat gttctccttc agcaggaatc acaatggtta ccttatcatc cttagctaga
    42721 gaaaacaaga cattttctac ttgatcatta actttgattt gaccgccttg aaattttatt
    42781 ttcgctaaca gaccctttga gatccctttt tcttttaaaa aatattttac ttgctgagaa
    42841 tgatctttct cataatccca tgtaaactgc atatcaatcc tcactaataa aggcatcttt
    42901 cacacgatgc caaaaatgca tatgtcgata agaagcaaaa tggatccgtt cttctgctat
    42961 tcgataataa attgagcgga tatttgcttt tgcgacattc aattgatcaa ccgttacaag
    43021 ataatcttct gtattttcca attttatttc tagccattca tcatgggcaa tcacgatcgg
    43081 agaacccaaa gtacgaaaca ctcgattatt cagtgaagca atctctgcca actgaaaggc
    43141 attgatacta ggatggatca cagcaccgcc gacactttta ttatatgctg tcgaaccagt
    43201 aggcgtagaa atcgtcaatc catctcctct aaaactttca aacagctcgt ttttgatgta
    43261 tacatcggct accatcgtac gattggcccg tttaatcgtt gattcattca gtgctaaaaa
    43321 atgctggtcc ggtgtttcat ccaaataact gatccgaaca tccagcaagg gataactaac
    43381 actttgttcc cggttcgttc gtaagctgtc tactagttct tccagctcgt aatcccgcca
    43441 atccgtgtaa aaccctaaat gacctgtatg cacgcccaag aaacgcacat cattcagaca
    43501 atgactgtag tgatggaacg ccgacaataa tgtgccgtct ccgccgactg agatgaccaa
    43561 ttctggctca tgatcatctc gttgaatccc tgcttgttct aaaagtgcgt tcaaacgttc
    43621 tgtcacttct atagtctttg cttctttatt atgaacgatc gctactttca tcccctgcct
    43681 cctctctttc gctttctttc ttcaaaagcg ttcgatagta gacatcatct gattttcctc
    43741 ttccatggga gaataaccgc tgtgcttctt ggatttcttc acggatactg gacatctctt
    43801 catccaactg ataggcagct tctgccgcac gctgaagtcg ttcactcatt tcttctggaa
    43861 aaacgccttg atatttataa ttcagcgaat gctcaatcgt tgcccagaaa ttcatcgcaa
    43921 gagtccgaat ctggatttcc gccaaaattt tcttctcacc gctgatcagc tgtacaggat
    43981 actcgatcac cagatgataa gaacggtagc cacttgcttt tttatttgtg atgtaatctc
    44041 gttcttggag aactttcatg tcttttcttt tacggatgat ctcaacaact tgatggatat
    44101 cttctacaaa ctgacacatg atccttaacc cggcaatatc agacatttct tcttctaaac
    44161 gtcccattgg aatatgccgt aattgtgatt ttgttacaat actatcgact ggcttgactc
    44221 tcccagtgac aaattcaatc gggacatgtc gattttgttc acggaactgt ttgcggatcc
    44281 cccgcagttt cactttcaat tctgatacag tttgttcata tggtgctaaa aatgcttccc
    44341 aatctcgttc catagctgat acctctactt cattaacatt cattgtttat tctaccatat
    44401 atgcaaagtt ttttcgtact cttgatttat gcattgacca aaaaaagaaa attaggcaaa
    44461 atagacaaaa aggagtcgat caaatgacac aaatgcttga aatcgaatat aaatctatgc
    44521 tgacaaagca agagtacgag atattcatcg ctcactatca attaacagat aaagatttcc
    44581 gtgtacagac taatatttat ttcgataccg ttgatgaaca gctgaagaaa caaaattgtg
    44641 gcctacggat ccgtttcgtt ggtcatcaag ctgaatatac attaaaaaca cctgctgaaa
    44701 aagggcgtct cgaaacaacg gataccttta taacagaaga agcagaagca tttatcttcg
    44761 aaaaacgtct gcctcgttca ggcgcagttt ttcaaaaact ttctgatttg aatatcgatc
    44821 ctgcttcatt aatccaaacc ggaaagctta caacaaaacg ggctgaattc cctattgaag
    44881 aaggactttt agctattgat gaaagctggg gagacaatct acacgatttt gaactggaac
    44941 tagaggtaag gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt
    45001 tctttacctt atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag
    45061 aaacctttca ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat
    45121 aggatgtgaa agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg
    45181 atcgaaatct atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag
    45241 ttattacaat ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc
    45301 tttcttcctt tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct
    45361 cagaacaatc ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac
    45421 tgcaaagcgg cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa
    45481 gaagcagtcg gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa
    45541 gaaatcggcg gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa
    45601 gtattctttt ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca
    45661 gtcgtttaca attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca
    45721 agtagccttt ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt
    45781 aatggtgaac atttacatac aaatgattct cagttccatt caagaactag ccatttacac
    45841 ttgctttcta attaaaaaaa tacgtcgact cccttacaag agagccgacg tatttttttc
    45901 gtttctattc gagaaaatca aaaatagaaa gccttataaa cgatcgatca attcttctaa
    45961 ttcatttaga cgttgttcaa acatcgacat tgcatcttca atatatgcag cctgtgtcat
    46021 atcaactcct gcttttttca ttacttccac aggataatcg ctgttacctg cttttaggta
    46081 agccaagtag ttctctagtg cctctggttc ctggtttagt attttcttcg ccagtgctga
    46141 agcagcagaa aagccagtcg agtattggaa aacatagtaa ttataataaa aatgcggtat
    46201 ccgtgaccat tcgaatttaa tttctgggtc ttcttcgact gctggaccat aatattttgc
    46261 attcaattta ccataactat cacttaggta ttcactagtc aatggcacac ctttttcatc
    46321 ttccgtatgc atgaaatgct cgaattcagc gaattgtgtc tggcggaaaa ctgttccttt
    46381 aaacccatcc aagtaatggt tgagtacata agcccgtaca cgagggtctt tttctgtttc
    46441 caataaatac tccgttagga tattttcatt cgttgtcgaa gcaatttctg ctaaaaagat
    46501 ggagtagtcg ccgtacacat aaggctggtt cgaacgagtg aaataactat gaacactatg
    46561 tcccatttcg tggacaagcg taaatagctg atccagcgta tcatgccaat tcaataaaat
    46621 atatggattt gtgtcatagc ttcccgaaga ataagcaccg cttcgtttcc ctttattttc
    46681 gacaacatcg atccaacgtt cagagaatgc tttttctacg atggccatgt attcttcacc
    46741 cattggtttc agtgcttcta aagctttttc ttttgcttct tcgtacgtaa aggtaattgg
    46801 agcttcccct aagaccggtg tataaagatc atacatgtgc agtttttcca cttctaataa
    46861 acgtttccgt aattccatgt atcgatgtaa caaaggcaaa tgcttgttta ccacgtctac
    46921 caaagtatcg tatacacttt caggaatatg attattgctc aaagacgctt ctctggcaga
    46981 gctgtaatta cggactttcg ctttaaaatt atgtcctttt atatgtgtgc ctaaagtaga
    47041 agcaaatgta tttctaaatt gttcgtaaac actgtacaat cccttaaatg ctgcttcgcg
    47101 cacccttcga tccgtgcttt ctagcaactg accatacacg ccatgagata attggactat
    47161 ttcaccattt tctccttcaa tcgttggaaa aactagatct gcattattca aaacagcaaa
    47221 tgtatcactt gaagcatcaa agatttcacc tgctccagca agaagagatt cctgctcagc
    47281 agataagaca tgggctcgat tatctactat ttgctggata taatggcgat agacttccaa
    47341 ttttggttct tctttgaaat attgccaaat ctggtcatct gacaattgca atatttctgg
    47401 ttcaaaccag gaaacggctt cactaacttt cgaaaacaga ctgcttgctc ttgcataaag
    47461 cgcttggtaa tctgtatttc cagtgtcttg atcgttttta aggtgcgcat atacataaat
    47521 gacttcagtt tggcgataaa ccctcaatac gaattcaatc gcatttaaaa attgagaagc
    47581 gccttgatca agtgttcctt tgtgtttttc agattgtttt aactcttctg ataattccaa
    47641 atatttctca tcaaactctt ggtcgcttga aaagatcttg gtcaagtccc aagttaaatt
    47701 ttcaggcaat tcttctcgtt ttggcaactg ctttacttcc atgaaatcca ctccttttct
    47761 tcccttagct ctatctatca aaatagatca tagtctaaca tccacatgtc acagcatcta
    47821 acttttctac tcattcatgt gtctgcttcc atcaatttta gcataactaa atcttgtttt
    47881 acatagaaaa ccgattccta gcctattttt atacttacta ttttttcaaa gaagacaagt
    47941 gtatgcattc gttaaaaaac tgctgataga ccatctcttc atcaatcata ggaaaaagcc
    48001 attccctttt atgttctttt atttggcaaa gccatgagtt gaaatcagga actttctggt
    48061 tttccaaggc ttcttcgtac aacattcgat aaagaaaaac ctgttcttgt aaataaaaga
    48121 aaaaccgact tttttgatag atccacgatg atagatgcaa aagatgttta tgacgcaagt
    48181 agcattgttc ttgtatggat actgtattct tttgcttgtg aataagttgg cgtgtcagcc
    48241 atttcttccc atcttcactc actttttcta tttttttcat ttggttcatt ttaatttcgt
    48301 tactagtgaa tatttcttta aggtccctac tagaaaaagt ccagcagcta ctgcaaaagg
    48361 aaatatgccc tgatacagtc tctgtaatat gatgatacaa ataaagcttt tgtttcttga
    48421 gatcagcttt ccagcaatgc actcctcttt ttctattata gtagcagcaa cttttttcta
    48481 tcaatgagaa ttgcgattgt ccaagaaaag aattaccaag tatccaccat acttggtaac
    48541 tgttattcaa ataattttgg gttctttcaa taaatctctg gtgagacagt ctagaacatt
    48601 ggatttctat tgctagattc gccaacaaaa tatccggcct ttgccgcaac ctcggcaaat
    48661 aggcttccaa aaatactggt tctactgact gtcccagcca atccatgaag cattccttta
    48721 actgtaagtg ctcttttgat tctctttcac taaagaccga acagttatct gatttttgat
    48781 gggcaaaatg agcacatttt ttctttccct ttttcaaaat cactcttttt cgacagccac
    48841 gacaataata tttctttcct ttttctgcat gtttagcaaa acagcactta ttatattcat
    48901 tcattgcaag cagcattttt ctccctctga acttatttac gtctaagaat ttatttacac
    48961 gaaaaaagag ggcgtgaaaa agctattctg catcaagtaa taagaacagc taaacaaaaa
    49021 tagttcccaa tatttttcgt ttggttgggc ttgttaccgc agatgcaagc ttttgaacac
    49081 atttatacga aaaaaagact gcgacacaat ttttgtcaca gtctcttact ataatgctga
    49141 tttatacaaa ataaaatttc gttcgctcga tagcattacg cgccatcaga cattgtccat
    49201 attcattcaa tgtttctggt gtacgatcgc tcttttcagc aaactccaaa attcgagcaa
    49261 tctgattctc tacatccgct tggttgagat tttccatcca gaagtgaacg actaggtaat
    49321 agcgctcttc gaataaatac aaatcagtcc aagcagactc tagttcgaca tctgttgcaa
    49381 gttggatcac ttgttcaaaa tcttcaaaag caaaagttgc ttgtctttcc tgttcttcta
    49441 gggcgcttcc agataattct tcctccggat cgctgccaat aatctgctga cgaagaaagt
    49501 cactcacatc ttctgaatca acagtagaat cttctgattg agaaatttct tcatccggaa
    49561 gatttttact gataaacagt tctaatccgt ctccttttgg caaaacttgg aaagtgacag
    49621 cttcgcttcc tcggaactcc tcttcgatat caacttcttc caaaatacta taaaagaaat
    49681 tttcgatttc tttgtgattg ccaagtaaat ccaaaaatga aacgccccgt tcctctaaat
    49741 cttcatgacc aattagtaca cggatcgtat tttcattaat atgttccatt tccattttgc
    49801 tacacctcac tttgccacaa tgtttaatat agattcattg tagcgaaatc agcttaaatg
    49861 taaagaaaaa aaatctcgct gttaagcgaa tttaataaaa ccagcggatt tttcaacctt
    49921 tcaagctatc ttatttaact ataacatttt tggcagacga atactatata gccgactttt
    49981 cagctaattt tccctctcta tttttcgtgc aatcatcatc ttctactacc tttttataca
    50041 tctaatcaag aatacttttt gtaaaaacaa aaaaacttgg aaaaactatc tttgtaatag
    50101 t
//
LOCUS       DAYYBG010000012        49125 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000012 DAYYBG010000000
VERSION     DAYYBG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.012"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..629
                     /locus_tag="ACHAUN_000667"
     CDS             291..629
                     /locus_tag="ACHAUN_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459392.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            723..953
                     /locus_tag="ACHAUN_000668"
     CDS             723..953
                     /locus_tag="ACHAUN_000668"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8459393.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            1353..1724
                     /locus_tag="ACHAUN_000669"
     CDS             1353..1724
                     /locus_tag="ACHAUN_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8459394.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(1998..2690)
                     /locus_tag="ACHAUN_000670"
     CDS             complement(1998..2690)
                     /locus_tag="ACHAUN_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8459395.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(2693..3478)
                     /locus_tag="ACHAUN_000671"
     CDS             complement(2693..3478)
                     /locus_tag="ACHAUN_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8459396.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            3715..5115
                     /gene="lacG"
                     /locus_tag="ACHAUN_000672"
     CDS             3715..5115
                     /gene="lacG"
                     /locus_tag="ACHAUN_000672"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8459397.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5140..5457
                     /locus_tag="ACHAUN_000673"
     CDS             5140..5457
                     /locus_tag="ACHAUN_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8459398.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            5470..7143
                     /locus_tag="ACHAUN_000674"
     CDS             5470..7143
                     /locus_tag="ACHAUN_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8459399.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            7157..8788
                     /locus_tag="ACHAUN_000675"
     CDS             7157..8788
                     /locus_tag="ACHAUN_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8459400.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(9279..10844)
                     /gene="guaA"
                     /locus_tag="ACHAUN_000676"
     CDS             complement(9279..10844)
                     /gene="guaA"
                     /locus_tag="ACHAUN_000676"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8459401.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10903..11826)
                     /gene="coaA"
                     /locus_tag="ACHAUN_000677"
     CDS             complement(10903..11826)
                     /gene="coaA"
                     /locus_tag="ACHAUN_000677"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8459402.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            12022..12627
                     /locus_tag="ACHAUN_000678"
     CDS             12022..12627
                     /locus_tag="ACHAUN_000678"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8459403.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12935..13951
                     /locus_tag="ACHAUN_000679"
     CDS             12935..13951
                     /locus_tag="ACHAUN_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8459404.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            14092..15393
                     /locus_tag="ACHAUN_000680"
     CDS             14092..15393
                     /locus_tag="ACHAUN_000680"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8459405.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            15439..16101
                     /gene="deoC"
                     /locus_tag="ACHAUN_000681"
     CDS             15439..16101
                     /gene="deoC"
                     /locus_tag="ACHAUN_000681"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8459406.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            16122..16520
                     /locus_tag="ACHAUN_000682"
     CDS             16122..16520
                     /locus_tag="ACHAUN_000682"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8459407.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            16586..17668
                     /locus_tag="ACHAUN_000683"
     CDS             16586..17668
                     /locus_tag="ACHAUN_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8459408.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17880..19442
                     /locus_tag="ACHAUN_000684"
     CDS             17880..19442
                     /locus_tag="ACHAUN_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459409.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            19435..20568
                     /locus_tag="ACHAUN_000685"
     CDS             19435..20568
                     /locus_tag="ACHAUN_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459410.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            20568..21524
                     /locus_tag="ACHAUN_000686"
     CDS             20568..21524
                     /locus_tag="ACHAUN_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459411.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            21623..22789
                     /gene="deoB"
                     /locus_tag="ACHAUN_000687"
     CDS             21623..22789
                     /gene="deoB"
                     /locus_tag="ACHAUN_000687"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8459412.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22813..23631
                     /locus_tag="ACHAUN_000688"
     CDS             22813..23631
                     /locus_tag="ACHAUN_000688"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8459413.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            23648..24355
                     /gene="deoD"
                     /locus_tag="ACHAUN_000689"
     CDS             23648..24355
                     /gene="deoD"
                     /locus_tag="ACHAUN_000689"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8459414.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(24653..25339)
                     /gene="gpmA"
                     /locus_tag="ACHAUN_000690"
     CDS             complement(24653..25339)
                     /gene="gpmA"
                     /locus_tag="ACHAUN_000690"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8459415.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(25511..26092)
                     /locus_tag="ACHAUN_000691"
     CDS             complement(25511..26092)
                     /locus_tag="ACHAUN_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8459416.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(26225..26908)
                     /gene="rpiA"
                     /locus_tag="ACHAUN_000692"
     CDS             complement(26225..26908)
                     /gene="rpiA"
                     /locus_tag="ACHAUN_000692"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8459417.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            27227..27640
                     /gene="rpsL"
                     /locus_tag="ACHAUN_000693"
     CDS             27227..27640
                     /gene="rpsL"
                     /locus_tag="ACHAUN_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8459418.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            27724..28194
                     /gene="rpsG"
                     /locus_tag="ACHAUN_000694"
     CDS             27724..28194
                     /gene="rpsG"
                     /locus_tag="ACHAUN_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8459419.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            28288..30375
                     /gene="fusA"
                     /locus_tag="ACHAUN_000695"
     CDS             28288..30375
                     /gene="fusA"
                     /locus_tag="ACHAUN_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8459420.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            30547..31734
                     /gene="tuf"
                     /locus_tag="ACHAUN_000696"
     CDS             30547..31734
                     /gene="tuf"
                     /locus_tag="ACHAUN_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8459421.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            32141..32449
                     /gene="rpsJ"
                     /locus_tag="ACHAUN_000697"
     CDS             32141..32449
                     /gene="rpsJ"
                     /locus_tag="ACHAUN_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8459422.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            32480..33109
                     /gene="rplC"
                     /locus_tag="ACHAUN_000698"
     CDS             32480..33109
                     /gene="rplC"
                     /locus_tag="ACHAUN_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8459423.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            33137..33760
                     /gene="rplD"
                     /locus_tag="ACHAUN_000699"
     CDS             33137..33760
                     /gene="rplD"
                     /locus_tag="ACHAUN_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8459424.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            33760..34050
                     /gene="rplW"
                     /locus_tag="ACHAUN_000700"
     CDS             33760..34050
                     /gene="rplW"
                     /locus_tag="ACHAUN_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8459425.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            34089..34922
                     /gene="rplB"
                     /locus_tag="ACHAUN_000701"
     CDS             34089..34922
                     /gene="rplB"
                     /locus_tag="ACHAUN_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8459426.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            34965..35243
                     /gene="rpsS"
                     /locus_tag="ACHAUN_000702"
     CDS             34965..35243
                     /gene="rpsS"
                     /locus_tag="ACHAUN_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8459427.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            35266..35613
                     /gene="rplV"
                     /locus_tag="ACHAUN_000703"
     CDS             35266..35613
                     /gene="rplV"
                     /locus_tag="ACHAUN_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8459428.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            35627..36283
                     /gene="rpsC"
                     /locus_tag="ACHAUN_000704"
     CDS             35627..36283
                     /gene="rpsC"
                     /locus_tag="ACHAUN_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8459429.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            36286..36720
                     /gene="rplP"
                     /locus_tag="ACHAUN_000705"
     CDS             36286..36720
                     /gene="rplP"
                     /locus_tag="ACHAUN_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8459430.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            36710..36898
                     /gene="rpmC"
                     /locus_tag="ACHAUN_000706"
     CDS             36710..36898
                     /gene="rpmC"
                     /locus_tag="ACHAUN_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8459431.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            36923..37189
                     /gene="rpsQ"
                     /locus_tag="ACHAUN_000707"
     CDS             36923..37189
                     /gene="rpsQ"
                     /locus_tag="ACHAUN_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8459432.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            37247..37615
                     /gene="rplN"
                     /locus_tag="ACHAUN_000708"
     CDS             37247..37615
                     /gene="rplN"
                     /locus_tag="ACHAUN_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8459433.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            37652..37960
                     /gene="rplX"
                     /locus_tag="ACHAUN_000709"
     CDS             37652..37960
                     /gene="rplX"
                     /locus_tag="ACHAUN_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8459434.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            37988..38527
                     /gene="rplE"
                     /locus_tag="ACHAUN_000710"
     CDS             37988..38527
                     /gene="rplE"
                     /locus_tag="ACHAUN_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8459435.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            38546..38731
                     /locus_tag="ACHAUN_000711"
     CDS             38546..38731
                     /locus_tag="ACHAUN_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8459436.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            38767..39165
                     /gene="rpsH"
                     /locus_tag="ACHAUN_000712"
     CDS             38767..39165
                     /gene="rpsH"
                     /locus_tag="ACHAUN_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8459437.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            39197..39733
                     /gene="rplF"
                     /locus_tag="ACHAUN_000713"
     CDS             39197..39733
                     /gene="rplF"
                     /locus_tag="ACHAUN_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8459438.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            39894..40250
                     /gene="rplR"
                     /locus_tag="ACHAUN_000714"
     CDS             39894..40250
                     /gene="rplR"
                     /locus_tag="ACHAUN_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8459439.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            40271..40771
                     /gene="rpsE"
                     /locus_tag="ACHAUN_000715"
     CDS             40271..40771
                     /gene="rpsE"
                     /locus_tag="ACHAUN_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8459440.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            40786..40965
                     /gene="rpmD"
                     /locus_tag="ACHAUN_000716"
     CDS             40786..40965
                     /gene="rpmD"
                     /locus_tag="ACHAUN_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8459441.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            41009..41449
                     /gene="rplO"
                     /locus_tag="ACHAUN_000717"
     CDS             41009..41449
                     /gene="rplO"
                     /locus_tag="ACHAUN_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8459442.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            41449..42744
                     /gene="secY"
                     /locus_tag="ACHAUN_000718"
     CDS             41449..42744
                     /gene="secY"
                     /locus_tag="ACHAUN_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8459443.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            42806..43453
                     /locus_tag="ACHAUN_000719"
     CDS             42806..43453
                     /locus_tag="ACHAUN_000719"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8459444.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            43644..43862
                     /gene="infA"
                     /locus_tag="ACHAUN_000720"
     CDS             43644..43862
                     /gene="infA"
                     /locus_tag="ACHAUN_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8459445.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            43895..44011
                     /gene="rpmJ"
                     /locus_tag="ACHAUN_000721"
     CDS             43895..44011
                     /gene="rpmJ"
                     /locus_tag="ACHAUN_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8459446.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            44029..44394
                     /gene="rpsM"
                     /locus_tag="ACHAUN_000722"
     CDS             44029..44394
                     /gene="rpsM"
                     /locus_tag="ACHAUN_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8459447.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            44422..44811
                     /gene="rpsK"
                     /locus_tag="ACHAUN_000723"
     CDS             44422..44811
                     /gene="rpsK"
                     /locus_tag="ACHAUN_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8459448.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            44891..45829
                     /locus_tag="ACHAUN_000724"
     CDS             44891..45829
                     /locus_tag="ACHAUN_000724"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8459449.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            45857..46237
                     /gene="rplQ"
                     /locus_tag="ACHAUN_000725"
     CDS             45857..46237
                     /gene="rplQ"
                     /locus_tag="ACHAUN_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8459450.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            46509..47348
                     /locus_tag="ACHAUN_000726"
     CDS             46509..47348
                     /locus_tag="ACHAUN_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8459451.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            47324..48193
                     /locus_tag="ACHAUN_000727"
     CDS             47324..48193
                     /locus_tag="ACHAUN_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8459452.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            48183..48980
                     /locus_tag="ACHAUN_000728"
     CDS             48183..48980
                     /locus_tag="ACHAUN_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8459453.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        15588 a         8897 c        10737 g        13903 t
ORIGIN      
        1 cgataagttg tgtattggaa ttttagatac agtaataaag tatttttcat gaaattttgt
       61 gagaatcact attacaagta tctttaataa aattaatggt taaggaggaa cttaaagtag
      121 aatgtaaatg aatagttgta gtcagtcgat tgtgatacta gactcaaagt tgcaggaata
      181 agtttttcaa tttgtgattg gaagataaaa aatgaaaaag aagggctttt ttttatactt
      241 tgttgattgt agtgtttatt ttccctctgt ttagccaagt aacattttct atgactcaac
      301 cagtttcaag tgaggaacat gataaccttt ttcgtgctac agaagagttt aagaaatatc
      361 aggaaaatat tgttccggag actccattag ctagtgagcc aattactgat attgatggaa
      421 accaaatagg atggtatata cagtacgaag ttatggtggt aaataatgac tcaatagtaa
      481 aaaatacagt aggcttagag ctaacaccga ttgaaaaggt gagttcattg ttaacgtata
      541 tttatactga tgaaagtgac gagactgaga tcattttaac ggattactca aaaattgcta
      601 ctgataaaaa gtatatattg tggatgtaga cactagtgaa atactttatg atatagaaat
      661 tgatgaaaat ttactagatg tagcacaaga aatcgatcat caaaagcagg aagctattcg
      721 atatgctggt agaaaaggga aaagaaaata tttattacgt gaatgtagca aaagttagag
      781 aagatgaaaa tgagtggaaa gaattcaaga gtcgctactc aattaactcg actccgacat
      841 ttacagttta cagagaagga tcgatagaaa aaactgtttt ttggactaaa gaaagtggaa
      901 tgtctttagc tgaagtagaa gagtttttgg attatgttag tatgcaacag taagggagtt
      961 cttattatga aaaattttga cacatattat gttattgttt tttcgatatc tttgctgtta
     1021 atattactaa ctttaatgca gtataaagag tatccagagt attttatttt aacttttgta
     1081 tatttagtaa gtagctacac acttctatat aaattcagaa gttcttctag agtttctaaa
     1141 gtttgtttct taattagctt attgattcta taatattttt gatttatata ggaaattaaa
     1201 aatattatag aatcaacact cagagaacgg gttggttttt aatagattta ataagtactg
     1261 ataatgaaat ttctcctggt tgggaaaacg aagttggtta ccctgtagaa gaattttttc
     1321 aagattactt tgcggagtat gccgatagga taatgagaga ttacgtaaca gataaaactc
     1381 aagaagtcat caatcaatcc cagtcattaa atgaattatc tgataaggcg aaagaagaag
     1441 cagttgccat tatttcttca gggattagtg actatattga tatgagtggt gaaatcaatt
     1501 atggcgataa agacaaaatt gtacgcatga taaaagcttt tatcaaaact tttgatgtga
     1561 aggcagatct taagtttaat gatacttacc tagtaggaaa attaattaat atccttggga
     1621 cagagaaaga tacggaaacg tttatttcct ctttgtctat gttgtttaca gataagcatt
     1681 tttcaactca gcttggtgga gaggaactag tggaaatctt ttaaccatta cgcccagcat
     1741 taataaaagt atatcaaaaa agtactcctg atgattttta agattggttt gagaaataat
     1801 gtgaaaacac tatttttaat gttaattata gatagagatg aacgcgtcat atggttaaaa
     1861 gaatgaaaaa gtcaaagata cactgctata tttcactaag gatatagcag tgtatctttc
     1921 atgtcgtttc actggcagtt ttttataaaa ggaggtgtgc atttcagttg cttttctttg
     1981 gtagttagtt gaatcgttta agaattaacg gatgtggcag acaggtgctc tgtccaaaaa
     2041 ccagtaaaat ctttgatggt tccatcttta tttagctgtg cctgttcaaa tactgttttg
     2101 tttttaccga atggatcaat tgcaataatt gttccgatcc ccgcacgttt ggcagcaagt
     2161 aatcctgagt aagcatcttc gataacaaga caatcagccg gagctaagct cagtttgtct
     2221 gccgctttaa gaaaaatatc cggttgcggt ttcccaggga aagagccgtc gtcatagaca
     2281 atcttgtccc agtcaaacca gcgggctaaa tcgaaaatat caaaataaaa agcaacattc
     2341 tctttgacag tagccgtggc aatcgttaat ggaatctgtt ggtcagtcag ctggtcaaga
     2401 gtcgcttcaa gcccttttgt caactgaagc tcgttttcat tttgcaagca aagcatacga
     2461 taatagcttt ctttttcttc tgacaatacg gcgatctcag aggcggtcaa atgctctgaa
     2521 atgaaatgtt tcaaaatctc cgtattcgtc cgaccatgaa tattttttaa aatatcctcg
     2581 tctgataaac catcttttgt atgatcatgt atcatatgca gccaagcagc ttcatgcaga
     2641 tgagagtcca aaaacattgt tccgttgaaa tcaaatataa tccctttcat agttacgctc
     2701 ctatagtggt attttcggtt gaatcagcaa tttgcagatc gacatattgt gcatagtgct
     2761 cttgaacctc ccgcgagatc tggcgatccg taatgagcag atcgaaatca tgcaaattat
     2821 aaaacacata aaaatcatct cgattgaatt ttttattatc aacgagtaga tatttcttac
     2881 gggaattatt tagagcgatc tgctgtgctt ctccttcttc ggtactgtaa gtagagatcg
     2941 cttcgttgtg aacgccattt gaactgataa atgcttttgt aaactttagt cttcttaaat
     3001 tgtcgttggt gatcggtccg acaaatgcgc cggtattttt ccggaaatca ccgccaatca
     3061 gtagaatttc tggtccatct gcttgagcaa gaatatcaaa aactggataa ttatttgtaa
     3121 tgatccgaat tttcttgttc agtaaatgcg tcgccaatag ttcgattgtt gtacctggtc
     3181 ctaagaaaat cgtttctccc tcgtgaatca aggaggctgc tgtggcagca atttcttttt
     3241 tttcttgaat ctgcagagtg gctttttcag tgtgagaaag ttcttgatct aaagtgtagg
     3301 aaagactttg agcccctcca tggacacgca gcagcttgcc cgatttttct aattcatcta
     3361 aatctctgcg aacggtcata tcagaaacat ctaattgatt gatgatttca ttaacggtaa
     3421 ggattccttt tttattgacc atttctacga tagtaagcaa tctttctttt ttcaacattg
     3481 tcttcacctc taatttaagg ataatctatc atataaacgg acaacagtca atctaattcg
     3541 aacaaaagta aacatttagg gtggttttta tgtttgttac aatagatttt attgctgttt
     3601 tctggttttt atgattattt gtgtttgttt ttgttgacaa atgttttgga tgcgtttaca
     3661 atgcaatcag ttagaaagag cgaaaactga ttatagattt gaggagagat gtacatgctt
     3721 aaattaccag aagattttat ttttggcgga gccacagcag cctatcaggt tgaaggagcc
     3781 actaaggaag gtggcaaagg cgctgttgct tgggatgact ttttagagga acaagggcga
     3841 ttcagtcccg atccggcaag tgatttttat catcaatatg caaaggacat tgaattgtgt
     3901 gaacgttttg gtgtaaatgg tctaagatta tccattgctt ggagccgcat ttttcctgat
     3961 ggagctggga aaccaaatcc agaaggaatc gctttttatc atcgtgtgtt cgaagaatgt
     4021 aagaaaagaa acgtaacgcc gtttgtgacg ttgcatcatt ttgatacccc taaacgtttg
     4081 tttgaccatg gagatttttt gaatcgagaa acaatagaag catttgtttc ctatgccatt
     4141 ttttgttttc atgaattcaa agaagtcaag gtgtggagta cctttaatga aatctatcca
     4201 gttgcaacca atcaatattt gttaggtgtt ttcccaccag gaatcaaata tgattttaca
     4261 aagatcgtcg cctgtcttca taatatgatg gttgctcatg cccgtgtagt gaactatttc
     4321 aaagaaaacg aacttccggg tgaaattggt gtagttcact cattagagac gaaatatgcg
     4381 gcaacagacg cgccggaaga taagcatgct gcctttttag acgatgcatt gtcgatccgt
     4441 ttcttattag atgctaccta tttaggctat tattcaactg aaaccttgac tgctcttgat
     4501 gagatatgtg aagcaaatca ggccagctat cattttcctg aagaagattt cgttgaattg
     4561 aaaaaagcat ccacgcgtaa tgactactta gggatcaatc actaccaatg ccattttgtc
     4621 aaagcttacg atggagaaaa tgcaatccat cataacggta cgggtgaaaa agggacctct
     4681 gtttataaag tgaagggaat cggagaacgt atttataaag aaggaattcc tcgaactgat
     4741 tgggactggc tgatttatcc agaaggtcta tatgacttgt tgctgcgaat taaaagcgat
     4801 tacccacatt acaacaaaat ctatatcact gagaacggga tggggtataa agatcagttt
     4861 gaagatggca ttattatgga tcagccgaga atcgactatt tgagagtcta tttggaatcc
     4921 ttaagcaagg cgataacagc cggggtaaat gtgaaggggt attttttgtg gtcgcttatg
     4981 gatcttttct catggacaaa tgggtataac aaaaggtatg gcttgtttta tgttgatttt
     5041 gaaacccaga agcgttaccc aaaagaatca gcttactggt acaagttggt cagtgaaacc
     5101 aaaacgatta tttaataagt gataatggag gaagaaaaga tgaataaaga ggaattacaa
     5161 atgcttggtt ttgagatcgt tgcatattca ggcgacgctc gcagtacatt attgaaatta
     5221 ttgaaagaag taagacaagg aaactttgaa catgttgatt cggcgttgaa agaagccgac
     5281 gaaaatttaa cccttgcaca taattcgcaa acaaaaattt tagcggaaga agcatctggg
     5341 aaagagatgg agatgggatt catttttatc catgggcagg atcatttaat gaccacttta
     5401 ttgctgagag atctgattca agactttatt gtactgtaca gacagaatca aggataggag
     5461 aagaacgcta tggatggaat cattaaacaa atcgaaaaag ggaaaccatt ttttgaaaaa
     5521 atctcacgta atagttattt aggagccgtt cgtgacggat ttttaacagc gatgccagct
     5581 attttatttt caagcatttt cattttgatt gctgcggttc ctgagatttt tggttaccaa
     5641 tggcctgaat ctgtctccac atggctatgg aaagtctata attattcgat gggtgttgta
     5701 gggttactcg tttcagcaac cactgctcgc tgcttggctg attcgatgaa tcgtcgctta
     5761 ccgaaaaaca aagtaatcaa caccacctct gtgatgctgg cctctgtggt aagtttcctg
     5821 ttgttaagcg tctctccgat tgaaggtgga ttttcaacgg aatatatggg gacaaaggga
     5881 ctactggctt cctttgtcgc tgcattttta acagttaatt tatataagtt ttgtgtgctg
     5941 aaagatatta ccattaaaat gcctaaagaa gttcctggaa cgatttcgca aaccttccga
     6001 gatatttttc ctttctcttt ttcagtattt gcggccgttt taattgattt aggcattcga
     6061 caaatcttcc aaatctcatt tgcagaagca atcattacgg tgctgcagcc gctatttaca
     6121 gcagctgacg ggtacttagg aatcgctatt atctggggag cgatggcgct gttttggttt
     6181 gtcggtgttc atggaccgtc gatcgtggag ccggcaattg cggcaattat ctatgccaat
     6241 gtggaaacca atctgcaatt gttcaattcc ggtgaacaag cttctaaagt actgacggtg
     6301 ggtttaggga attttgtagg aactatggga ggaactgggg caacattggt tgttcctttt
     6361 atcttcctac tctttgctaa gtctaagcag ttgaaggcag ttgggaaagc ttcctttatt
     6421 ccagtaagtt tcgctgtcaa cgaaccattg ctatttgcgg cacccatgat tttgaaccct
     6481 tattttttta ttccattttt gttaacacct gttgtaaacg tttgcctgtt caagtttttt
     6541 gtcgatgtat tgggcatgaa cagctttatg tacgttttgc cttgggcaac tccagcaccg
     6601 atcggtttgg ttctaggaac gggcatgggt atcttagctt ttgttctagc agcggttcta
     6661 atcgtcgttg acttcctgat ctatctacca ttttgcaaag catacgatgc tgtcttagta
     6721 gagaaagaag cacaaaaagc agaggtgatc gcaaccgagg aaggtgcttc tgccgatgta
     6781 cgtgaggaag tacctgtggc agcgagtcaa gttatcagcg atgcaaataa agaaatcaat
     6841 gtgttggtac tttgtgctgg cgcagggaca agtgcgatgt tagctaatgc tttaaaaaaa
     6901 ggtgcatctg aatcgaaagc accgatcagt gcaatggcgg gtgcttatgg ttctcattac
     6961 gaaatgctgc aggattttga tatggtggtc ttagcaccgc aagtcaattc ttactatgat
     7021 gatatcaaag aagacaccga tcgcttagga atcaagctgg ttgcgacaaa aggagcagaa
     7081 tatatcggat taacgagaga tcctgaaaaa gctgttcagt ttattctgaa ccaatttcaa
     7141 taaaggagga gccataatga aaatgggaat ttggttgcgt aaagctgttt tatttgcagc
     7201 tgtgttaggg gctttggggg ctgtttcaaa aactgttgaa gcatctgcca gcgaatttat
     7261 tcgcggagca gatatttcga tcctgtcaga tatggaaaaa tctggtgcaa aatattatga
     7321 agacgatatt tcaaaagatg ccttagagat tttaaaaaat aatggtgtca actatgttcg
     7381 actgcgtttg tggcaggatc cttataatag taatggagaa agttatggag cagggacaaa
     7441 tgatctggaa acgacgattg accttgcaaa acgagcaaaa aatctgggac ttaaggtgct
     7501 gctggatttt cactacagtg atttttgggt tgatcctggg aaacagaatc ttcctaaagc
     7561 atggcaaggt ctgacctttg aagaaatgaa tacggcattg tacgactata caaagaacgt
     7621 tctgtcggaa atgaagcagc tggacgttta tcctgatatg gttcagatcg gcaatgaact
     7681 gaatagcgga atgctctggc cttatggcaa gtcttgggga gaaggcgggg gagaatttga
     7741 tcgattggct gcttttttga agtctggtat ccaagctgtt cgagacactc aacctaaaac
     7801 aactccagta atgctccact tagctgatgg tggcgataca ggagcattta cttggtggtt
     7861 tgatgaaatc acgagtcgag gtgtgtcatt tgatttaatt ggagtgtcgt attatccgta
     7921 ttggcacggt tcaatgaatg aacttcaaac caatatgaat gcgatcagcc aacggtatgg
     7981 taaagaagtg atcgtagttg aaacagctta tgggcataca acagcgaatg ccgatactat
     8041 gccaaatgct tttggtgaag ccgaagcagc tgcgggggga tatgaaccaa caccgacagg
     8101 acaagcagag tatttgctgg atcttgccga tcggattcag gcagtaccga ataatcgcgg
     8161 ggcgggcttc ttctattggg aacctctttg gtacaacgga aatgtgagtt gggcaacaca
     8221 agcaggtatg aattatctca gtgttcaatc cactatggga aacgaatggg acaaccaagc
     8281 gatgtttgat tttgctggaa acgccttgcc aagcttgcgt gcgtttaaac aagcaggggc
     8341 gcaaataaat ctagttaaaa atgcaagttt cgaaagcgat ggaccaacga ccagtccttc
     8401 caattggcaa acctggtttc aacaaggaac cgctaagggt acgatcaaaa cggaagcggg
     8461 cacttttttt ggggactaca aactaacttt ttggcaggat caagcatacg aggcatcagc
     8521 ttatcaaaat gttagtggca taagcaaagg aacttacaca ttgagtgcct atgtaatgtc
     8581 tgggggagag tttgatacta atcagttata tatcaaaaat tatggtggag cggaactaaa
     8641 tactgcggtt gttaaaacaa gtgaatggac aaaaattaca atcaaagaca ttccaattac
     8701 aacaggtgtt tgtgaaattg gggtttatag caaggcttca ggaaatgctt ggacgaacgt
     8761 tgattttgtt tcattggtga aacaatagac tgcggatcgt ttcgattcaa gatcggaggt
     8821 tcccttcagc cacatcgtta gcgtagaagg caggaatcga acctgcacct cgcctttgcc
     8881 aaaaataacc agaaacgatt tccagtcatt tttaccaaaa cgagtaaagc accagacttc
     8941 atctacactt tgtattatgc taaagagagt tcaacgattt cgtaagaagc aatgaaaaaa
     9001 ttgcaaaaag ttgtgcagat aacataaagt ttgtttaaga aagggtaacc ttagaattcc
     9061 gtcgtgaata tgatttgagc acttagatgg atagaaaaaa gttctcggaa aacagttcgt
     9121 ttttcgagaa cttcttttta tgataagaat gaaacaaatt aacttatatt atagtctctt
     9181 ttggtaggga accatcttat ttttaatgaa aatcaagcat tttagctatt gaagaatgtt
     9241 gatttatagg gattccccct ccatattatg ttggctcttt attcccactc cactgttgct
     9301 ggtggtttag aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata
     9361 cgtacagaga ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg
     9421 tcgattgaag ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata
     9481 acaccgacag agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt
     9541 ccagcagctg cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg
     9601 tcttcagtga tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc
     9661 caaacgattg aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat
     9721 aatgtgttta atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta
     9781 tggtgagatt tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac
     9841 gttccttgtg ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca
     9901 aaaacatata cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct
     9961 ttcaacttgt ctaagaagcg atctcttgca ttgactttga tgatgttcaa gccgaatttt
    10021 ccgcctagac tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca
    10081 aagatagatg tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa
    10141 tcaacgccac ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt
    10201 tctacttgca tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca
    10261 aaggcaaagt ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat
    10321 tggataccat aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt
    10381 gttccgactg tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat
    10441 actgtttgtt gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac
    10501 aaggctttcc catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg
    10561 atcaattgca taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga
    10621 tcgatcccaa atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc
    10681 tttggattca tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag
    10741 actccaagat cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat
    10801 acgataatct tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc
    10861 cctttaaatt ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa
    10921 aatcgaatcg atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt
    10981 aggcaagata tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat
    11041 tttaaaggct tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg
    11101 gaaagccgta tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg
    11161 atcggcatcg acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc
    11221 aggtaattga agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc
    11281 gtacttgtct ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc
    11341 ctgaccagat tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg
    11401 aaatccttta cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc
    11461 gtcagtcgtg atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag
    11521 cagtctggct gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg
    11581 cggtacttct acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc
    11641 tttcatataa agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat
    11701 ggaaatgcgg tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg
    11761 cccattacga taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc
    11821 attcatatta gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc
    11881 aaaccccacc acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa
    11941 acggagtctt attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa
    12001 gaaatgagaa ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg
    12061 cacgattttg aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat
    12121 tctggtgtat tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc
    12181 gattgggaag agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg
    12241 attgggttag ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa
    12301 cgagcggtaa gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc
    12361 catcaatcga atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat
    12421 ccgccgatcc gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg
    12481 ttgaagaagg gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg
    12541 caaaagaaaa tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat
    12601 tatatcatca aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg
    12661 gaactgggcg tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa
    12721 acaaagaact attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga
    12781 taaaataaat aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt
    12841 ttttatcaaa aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac
    12901 tgatagggaa tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc
    12961 agaaatggca ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag
    13021 atttagtgat aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc
    13081 cgattatttc gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc
    13141 tgatgtcact gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc
    13201 tttgggctat gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta
    13261 tttacaagag ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt
    13321 acctgagaaa tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga
    13381 tcgcggattg aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta
    13441 tcaagctgtt tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac
    13501 cactggatac tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca
    13561 tggcctgcct tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata
    13621 tactgcatca aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga
    13681 tgccatggcg atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga
    13741 tatctcagtc ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac
    13801 gacagtgcag cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac
    13861 gatcgaattt cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg
    13921 tgagagcgta aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt
    13981 tacaaaggct agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac
    14041 ctttcctaaa aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg
    14101 gtggatctga ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac
    14161 atcgtcacga actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg
    14221 gcaatttttt atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct
    14281 aattctggtg atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg
    14341 acaggtggcg ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga
    14401 gtgacagtgg ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag
    14461 ctagaagcaa ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt
    14521 aatgaaagta aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa
    14581 ttgtatgcct tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg
    14641 attatgagta aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga
    14701 gacggtgctt tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc
    14761 attgggaaac tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt
    14821 ggcgaagcta tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat
    14881 ggtccagaag atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct
    14941 ggcaaagcta agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt
    15001 aaagcgttag caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg
    15061 gaacatccag aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc
    15121 ggagttgtgt caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct
    15181 gggcgtaaaa ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa
    15241 attggtgaca ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa
    15301 atcacttctg tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag
    15361 ccaacgttga ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta
    15421 aagaaaaggg gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca
    15481 gacgcatcta aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat
    15541 tctgtctgca tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca
    15601 gtagccgtat gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca
    15661 tttgaaacaa cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc
    15721 ggtgctttga aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa
    15781 gcagcaaaag atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa
    15841 gaaatcatcc gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca
    15901 actggtttct caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta
    15961 ggaccagaaa tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca
    16021 atgatcgaag ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt
    16081 tcaactggtg aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag
    16141 aatggatcaa cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc
    16201 cggtaggcgc ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga
    16261 atgcatcatt tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg
    16321 aaggagaaaa aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc
    16381 cttgtggtgc atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc
    16441 tagtaggaga caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat
    16501 ttactgaaaa agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac
    16561 aattatttta ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt
    16621 cgcactagca gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc
    16681 cggtggcaaa agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt
    16741 agacgaccgt tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca
    16801 aggactttca cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta
    16861 catcccaaat attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta
    16921 taaactaaaa ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat
    16981 tatcgatgat gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga
    17041 agcttcttat cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt
    17101 tatcggcggt gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt
    17161 tgatgcaggt gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga
    17221 cttctctgct ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga
    17281 tatcatcttc catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt
    17341 gaacgaatca ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga
    17401 aggcgaatac acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc
    17461 agttggaaca gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg
    17521 cgaacacact gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc
    17581 tgacgaagcg aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa
    17641 agttccagaa actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt
    17701 tgacaaggct atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac
    17761 agtttggata agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact
    17821 aagcgtgaat cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg
    17881 tggttcaaga aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca
    17941 aggcaaatga caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag
    18001 aaaacggtgc tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat
    18061 cagggaaaat tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc
    18121 aattagggat tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag
    18181 aaaacatcgt gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac
    18241 gaaaagagat ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg
    18301 ttcgtgatat ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag
    18361 gagcagatgt actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg
    18421 aattaatcgt gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc
    18481 ataagttaga tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag
    18541 gaatcggtac agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg
    18601 gaagaacggt ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt
    18661 ctattgaaaa tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact
    18721 tagaggttcg tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg
    18781 agttgatcca agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag
    18841 gggaagacat cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc
    18901 cagaagaccg tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac
    18961 tacaaaccta tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga
    19021 atgaacatgc cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc
    19081 ccgcaaaagc tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc
    19141 gagatcctga tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg
    19201 aatttattca taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca
    19261 gtttcgaatt agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag
    19321 aaattgtcgg tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg
    19381 cgggctatac cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat
    19441 gatcgcagcg aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg
    19501 atcttaggcg cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct
    19561 atgctagatg cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg
    19621 ccgttgattt ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt
    19681 gggctttctg gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt
    19741 ccagatctgc caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt
    19801 gcgatggcag cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc
    19861 gtaacaatca tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg
    19921 atgcctgaaa gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca
    19981 ctgcggttac catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt
    20041 ttagcaatca tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt
    20101 gaaatccgtt ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa
    20161 cgtacgatcg ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt
    20221 gaaggattag gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt
    20281 gacggaatgg ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg
    20341 atcttgttca gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg
    20401 tttgagatcg tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg
    20461 attcgtttcg tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca
    20521 atagaatcga aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg
    20581 aattaattgc cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt
    20641 cactaggcgg aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca
    20701 tggtcatggg tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt
    20761 ctatgacacc atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga
    20821 ttcacgcagt agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta
    20881 atctgatggc acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa
    20941 ctgataacat cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg
    21001 tcattggacc aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg
    21061 ctgtcctagc ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg
    21121 gtgaaaatcc tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag
    21181 gcgtactgat ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt
    21241 ccggtaactt ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga
    21301 tctttggtaa gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc
    21361 aaagcttgag tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc
    21421 tgcaaattgc tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt
    21481 ctggtccaaa agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa
    21541 aagggtgggt tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa
    21601 taaaaagaaa gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt
    21661 aggaattgga gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg
    21721 acacattgcg aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac
    21781 gatccgtcct cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt
    21841 agaagaagtt tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa
    21901 tatcaaaact ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat
    21961 cgaagaattt tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat
    22021 tgacgattac ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga
    22081 tcctgtcctt cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat
    22141 ttgccaattc gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc
    22201 acgtccgtac gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc
    22261 gttgaatcca ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat
    22321 tgctgttgga aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac
    22381 gaaaagcaat atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg
    22441 aatcagcttt accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt
    22501 cggctatgcg catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat
    22561 ggaagaagac gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg
    22621 taccgatcat acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca
    22681 aggggcattg cctcaaggat attattcaga tatttcagca acgattgctg agaactttag
    22741 tgtttcttcg actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat
    22801 aggagaaagc aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt
    22861 gataaaggag taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta
    22921 gcagatgaaa tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt
    22981 tcgacagtag ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg
    23041 ttagccatgc aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat
    23101 ccagtacgtg tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt
    23161 ggtgtcaacg aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt
    23221 actggtgaaa atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat
    23281 atgagtcaag cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta
    23341 ggtttggatc ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct
    23401 gcggagatcc gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca
    23461 gaagtcatcg tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac
    23521 ttagcagcag gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga
    23581 gtcaaacaat cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa
    23641 caataaaatg agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact
    23701 tccgggagat cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg
    23761 ttacaatcag gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc
    23821 tgttcaagga acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca
    23881 gtcgtatggt gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt
    23941 ccatgttcgt gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa
    24001 aaatttccaa gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata
    24061 tgagatagct aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc
    24121 tttctacaaa gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt
    24181 ggaaatggaa gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag
    24241 tttgatgacc gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca
    24301 atctacgttc aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg
    24361 aaatgaaaaa gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat
    24421 gtttttgact tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag
    24481 aggttgtgaa tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt
    24541 catgtttgat gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac
    24601 gaaaaaaggg actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt
    24661 agtatttttt agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg
    24721 gaatttcaag atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc
    24781 gtaatgagtt accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa
    24841 tttcatcttg ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag
    24901 gaatatcgcg tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag
    24961 tttcttccat taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt
    25021 cgccgtattt ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt
    25081 cgtttaagcg ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca
    25141 agttacatgt tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc
    25201 cagcttcttt gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat
    25261 ttacatcagc ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag
    25321 aaaaaactaa ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt
    25381 ttcagttctg ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag
    25441 attctatgag aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg
    25501 tttttaggta ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat
    25561 aacatgttcc aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt
    25621 cataatagag acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga
    25681 tttttccttt tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc
    25741 actccatgtt tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat
    25801 cgattccttc atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt
    25861 ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc
    25921 cttgttgcat gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca
    25981 cttcatatgt ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag
    26041 ttgttaaatc aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc
    26101 ctttctagca gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca
    26161 aaaagatagt tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt
    26221 ttctttattt acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta
    26281 ctttagaaac catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat
    26341 aggtagctaa gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac
    26401 caccatctgt ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt
    26461 tttcttcaaa aatatgaagt agctgttggg agccataagg gatcacttca actgggagtg
    26521 ggaacttccc aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg
    26581 agtacgtagc gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa
    26641 aatcagcact gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa
    26701 tactctttaa cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa
    26761 ctcctgtgat acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat
    26821 aggcggttga accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt
    26881 caatgccagc taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc
    26941 gtgctttcct cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc
    27001 aataaagctt tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat
    27061 tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa
    27121 gcatatttga taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct
    27181 ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa
    27241 ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa
    27301 caaaggatat aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg
    27361 ggtagcaact cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata
    27421 tgcacgtgtt cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca
    27481 taacttacaa gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag acttaccagg
    27541 ggtacgttac catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca
    27601 aagccgttct aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg
    27661 aaacttgctt ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta
    27721 cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac
    27781 tcaaaattag taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca
    27841 gctaacatta tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta
    27901 gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt
    27961 gttgggggtt caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta
    28021 ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc
    28081 ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa
    28141 gacactcata aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct
    28201 ctctgtttgg tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag
    28261 atagtaaaga gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt
    28321 aatatcggta tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg
    28381 tactatactg gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac
    28441 tggatggaac aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa
    28501 tggaagggtc accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa
    28561 gttgaacgtt cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt
    28621 gttgaacctc aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc
    28681 gtatttgcaa acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta
    28741 catgaccgtt tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac
    28801 ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga
    28861 acagagatcg aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc
    28921 gaaaaattgg ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa
    28981 ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta
    29041 gaattctacc cagtactatg tggttcagca tttaagaaca aaggggttca attactattg
    29101 gacgctgttc ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac
    29161 cctaaaactg atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta
    29221 gcatttaaag ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct
    29281 ggtgttttac aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt
    29341 ggtcgtatcc tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga
    29401 gatatcgctg ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa
    29461 aagagtctag ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt
    29521 gaacctaaat caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa
    29581 gaagatccta ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt
    29641 atgggtgaac ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa
    29701 gctaacgtag gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc
    29761 gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa
    29821 tttacaccaa atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt
    29881 gttcctcgtg aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt
    29941 gtcttagctg gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat
    30001 gatgtcgatt caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca
    30061 gctaagaaag ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct
    30121 gaagattatt taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt
    30181 atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg
    30241 tttggttatg ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca
    30301 tttgaccact acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc
    30361 ggaaaagctg aataatttct taaaaccgga tagtcctgca aagagattat ccaaagagac
    30421 tatattttta atatagtttc gtgtaaaata attatcgatg atatgggcat gtggtgtacc
    30481 ttgtgtacat catataccta tcagacaaat aaaaaatata ttgtattttt aggaggacat
    30541 tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc
    30601 ggacacgttg accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag
    30661 aaaaacggcg gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa
    30721 cgcggaatca caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct
    30781 cacgttgact gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa
    30841 atggacggag ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa
    30901 cacatcctat tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta
    30961 gacatggttg atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta
    31021 acagaatacg aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct
    31081 ctagaaggcg acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa
    31141 tacatcccaa ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg
    31201 ttctcaatta ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc
    31261 gttggtgacg aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact
    31321 ggtgttgaaa tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct
    31381 ttactacgtg gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt
    31441 acaatcacac ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt
    31501 ggacgtcata ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac
    31561 gtaacaggtg ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta
    31621 acaatggaag ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc
    31681 cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact
    31741 taagttttaa agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtcttagg
    31801 atctttgttc cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata
    31861 aatagaagaa acacgaaata aaagagtcag tattttctaa atgaaacttt atgaaaacaa
    31921 aatgtttttt acaagaaaaa gccttgcttt gtgagtttat atatattata atgacaaagg
    31981 tgcgtttact ttagtgaatt gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg
    32041 agaggttgcg acacgcccgg acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga
    32101 gctatgtcta cttttcaaaa taggcgaagg agggaacaag atggcaaaac aaaaaattcg
    32161 tatccgttta aaagcgtatg aacaccgtat tttagatcaa tcagcggata aaatcgtgga
    32221 aactgcaaaa agaactggag ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag
    32281 tctttacaca atcatccgtg cgactcataa atacaaagat tcacgcgaac aattcgaaat
    32341 gcgtacacac aaacgtctta tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt
    32401 gatgaagcta gacttaccat ctggtgtaaa catcgaaatc aaattataaa actaaaacaa
    32461 aaataatgga ggtgtactca tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca
    32521 aatcttcact gaatctggtg aattaattcc agtaactgtt gtagaagcta ctccaaacgt
    32581 agtactacaa gtgaaaacaa tggaaacgga tggctacgaa gctattcaag ttggttacca
    32641 agatatgcgt gaagttttat caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac
    32701 ggctcctaag cgcttcattc gtgaattcaa aaatgttgag ctaggagaat atgaagtagg
    32761 aaaagaaatt acagtagatg ttttccaagc aggagacatt attgatgtaa caggtactac
    32821 gaaaggtaaa ggattccaag gggttatcaa acgccacgga caaagccgcg gaccaatggc
    32881 tcacggttct cgttaccatc gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt
    32941 atttaaaaac aaacgtcttg ctggccgtat gggtggcaac cgcgtaacta tccaaaactt
    33001 ggaagttgta cgtgtcgatg cagaaagaaa tgttatcttg atcaaaggta atgttcctgg
    33061 agctaaaaaa tctttaatta ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga
    33121 aaggaggaac taaggaatgc cgaatgtagc attattcaaa caagatggaa gccaaaacgg
    33181 tgaaatcaca cttaacgaag aaatcttcgg aattgaacct aatgaaagtg ttgtttacga
    33241 tgcaatcgta atgcaacgtg cgtcattaag acaaggaaca cacgcggtta aaaaccgtag
    33301 cgctgttcgc ggcggcggac gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca
    33361 aggatctatc cgctcaccac aatggcgtgg aggtggcgta gtctttggac caacaccacg
    33421 ttcttacagc tacaaacttc ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc
    33481 agaaaaagtt gctgaaaaca acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa
    33541 aacaaaagaa ttcaaacaag ttcttgctaa cttatctatc gactctaaag tattggttgt
    33601 tctagaaaaa ggtaacgact ttgctgcttt atcagcacgt aacttaccaa acgtttctgt
    33661 agtaacttct gataatgtga gtgtgctaga cgttgtatca aatacaaaag tgttagcaac
    33721 acaaactgct cttactcaaa ttgaggaggt gcttgcataa tgaacttact agacgtaatc
    33781 aaacgcccag tgatcactga aaaatcaatg cttgccatgg atgacaagaa atacactttt
    33841 gaagtggaca ctcgcgcaaa caaaacttta gtaaaacaag cggtagaagc tgcttttgac
    33901 gttaaagtca aaaacgtgaa catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa
    33961 tatgcaggat atacaaaaaa acgtcgcaaa gcgatcgtta cattgactga agattcaaaa
    34021 gaaatccaat tattcgaagc tgctgaataa gcactaatag ataactcatt aaattaggag
    34081 ggaaaaacgt ggcgattaaa aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag
    34141 gttctgattt tgctgaaatc acaacatcaa cacctgaaaa atcattgtta caaccattga
    34201 aaaaccatgc tggacgtaac aacaacggcc gcatcactgt acgtcatcaa ggtggcggac
    34261 acaaacgcca atatcgcgtg atcgacttca aacgtaacaa agataacgtt gttgcaactg
    34321 ttcaaacgat cgaatatgat ccaaaccgtt cagctaacat cgcgttagta cactatgaag
    34381 atggagtgaa agcttacatc ttagcaccta aaggactaga agtgggtatg aaacttgttt
    34441 caggtccaga agcagatatc aaagtcggaa atgctcttcc attagaaaac attccagttg
    34501 gtactgttat ccacaacatt gaaatgaaac ctggtaaagg tggacaattg atccgttctg
    34561 ctggtacaag tgctcaagta cttggtaaag aaggcaaata cgtattgatc cgcttgaact
    34621 ctggtgaagt tcgtatgatc ttagctgcat gccgtgctac aatcggttct gtaggtaacg
    34681 aacaacacga attaatcaac atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc
    34741 caactgtacg tggtagcgta atgaacccta atgatcaccc acacggtggt ggtgaaggta
    34801 aagctcctat cggacgtaaa gcaccagttt ctccttgggg tcaaccagct cttggttaca
    34861 aaactcgtaa caaaaaagct aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat
    34921 aattactttg accatgtgtc gcacattttg agaggaggtt caccatgggt cgtagtttaa
    34981 agaaaggacc ttttgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg
    35041 aaaagaaaaa agttatcaaa acttggtcac gccgttcaac agttttccca agttttgttg
    35101 gatataccat cgcagtttat gatggacgta aacatgtacc agtttacatc caagaagaca
    35161 tggtaggaca caaattaggt gaatttgcac ctacaagaac ttatcgtgga catgctgctg
    35221 acgataagaa aacgaaacgc taatttgagg ggaggataaa tccaaatggc agaacaaatc
    35281 acatcagcta aagcaactgc aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta
    35341 attgacctta tcagaggtaa aagtgtcgca gatgcaattt caatcttgaa attcacacca
    35401 aacaaatctg ctggaatcat tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat
    35461 aactttgact tagatgttga aaacttggta gtaagcgaag cttttgttaa cgaaggacct
    35521 acaatgaaac gtttccgtcc tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca
    35581 agtcatatca cggtagtcgt atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt
    35641 acatccaatt ggaatgcgtg taggcatcat ccgcgactgg gatgctaaat ggtatgctga
    35701 aaaagagtat gctgagttct tacacgaaga tttaagaatc cgtaaattta tttcaactaa
    35761 acttgctgat gctgctgtat caacagttga aatcgaacgc gcaacaggtc gcgtaaacat
    35821 ttcaattcac acagctaaac caggtatggt tattggtaaa ggcggttctg aagtcgaaag
    35881 cctaagaaaa gaattaaaca aattaactgg taaaagaatc catatcaata tcgtggaaat
    35941 caaaaaacca gatctagatg caaaattagt aggtgaagga atcgcacgtc aattagaaaa
    36001 ccgtgttgct ttccgtcgcg ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc
    36061 tcaagggatc aaaactcaag tatctggtcg tctaaacgga gcagatatcg ctcgttcaga
    36121 aggatactca gaaggaactg ttcctcttca tactttacgt gcggacattg attacgcatg
    36181 ggaagaagca gatacaacat acggaaaact aggagttaaa gtgtggatct atcgtggaga
    36241 aattcttcca actaaaaaaa acactgagaa aggagggaaa taatcatgtt agtacctaaa
    36301 cgtgtaaaac accgtcgtga attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa
    36361 gaagtagcat tcggtgaata cggtttgcaa gctgttgatt cacattggat cacaaaccgc
    36421 caaatcgaag ctgctcgtat cgcaatgact cgttacatga aacgtggtgg gaaagtatgg
    36481 attaaaattt tccctcacaa atcttatact gccaaagcaa ttggggtacg tatgggttct
    36541 ggtaaagggg cacctgaagg atgggttgca ccagtaaaac gtggtaaaat catgtttgaa
    36601 atcgcaggcg ttcctgaaga agtagctcgt gaagcgttac gtctagcttc tcacaaatta
    36661 ccaatgaaaa ctaagatcgt aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa
    36721 agaaatcaga gaattaacca ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga
    36781 attgtttaat cttagattcc aattagcaac aggtcaatta gaaaacactg cacgtattaa
    36841 agaagtacgt aaatcgattg cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt
    36901 atggaaggag gccattaagc gtatgactga aggaagaaat caacgcaaag tttatcaagg
    36961 tcgcgtggtt tcagacaaaa tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa
    37021 ccaccctatc tatggcaaac gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa
    37081 caacacagca aaagttggcg acatcgtgag aattatggaa actcgtccat tatcagctac
    37141 aaaacgtttc cgtttgttgg aagtagtcga agaagcagtt attatctaat aacacgagat
    37201 tttcctatat agattgaatg caaaatctga aaggaggata cacaacgtga tccaagcaga
    37261 aagtcgttta aaagtcgctg ataactcagg cgcacgtgaa attttgacga tcaaagtcct
    37321 tggtggatct ggtcgtaaaa ctgctaacat cggtgatgtg atcgttgcta cggttaaaca
    37381 agcaacgcca ggtggggttg tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac
    37441 aaaatcagga gctcgtcgta cagacggttc atatattaaa tttgatgaaa atgctgcagt
    37501 gattattcgt gatgataaaa gccctcgtgg aacacgtatc tttgggccag ttgcacgtga
    37561 actacgtgaa aacaacttca tgaagatcgt ttcactagca ccagaagttt tataatcttg
    37621 agacacttat caaaggaggt gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa
    37681 agttatcact ggaaaagaca aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa
    37741 agataaagtt attgtcgaag gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc
    37801 tgctcctcaa ggtggaattg ttgagatgga agcgccaatt catgtttcta acgtgatggt
    37861 ggttgactct actggtgtag ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt
    37921 ccgtgtttct aaaaaaaccg gtgaagtttt agataaataa aagtttaagg aaggagggtc
    37981 ttactgaatg aaccgcctaa aagaaaaata tcttaaagaa gtaactccat cattgatgga
    38041 aaaatttaac tatagctctg ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat
    38101 gggtgttggt gatgcggtat caaacgctaa aaacttagat aaagcagttg aagaattaac
    38161 tttgatcact ggtcaaaaac caatgattac aaaagctaaa aaatcaatcg ctggattccg
    38221 tttacgtgaa ggaatgccaa ttggtgcaaa agttacattg cgcggagaaa gaatgtacga
    38281 attcttagac aaattggttt ctgtttcact accacgtgta cgtgacttcc atggtgtaag
    38341 taaaaaagcc tttgacggtc gtggaaacta tactttaggt attaaagaac aattgatctt
    38401 cccagaagta gattatgatt tagtagataa agtacgtggt atggacatcg ttattgtaac
    38461 aacagcaaac tctgatgaag aatctcgtga gttgttgaca caattaggca tgccattcca
    38521 aaaataatta aaggaggcga actacgtggc taaaaaatca atgattgcta aaaacaaacg
    38581 tcctgcaaaa ttttcaacac aagcttatac tcgttgcgaa cgttgcggac gtccacattc
    38641 agtttatcgt aagttccatc tttgccgtat ttgcttccgc gaacttgcct ataaaggtca
    38701 aattcccggc gtgaagaaag ctagctggta aaaagtaaat tcgcataaga aggaggtaaa
    38761 agatcaatgg tcatgacaga tccaattgca gattttctaa ctcgtatccg taacgcaaac
    38821 atggttaaac atgagtcttt agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa
    38881 atcttgaaac gtgaaggttt cgttcgtgat gttgaataca tcgaagatga caaacaaggc
    38941 gtgatccgtg ttttcctaaa atttggtaaa aacggcgaac gtgttatcac aaacttaaaa
    39001 cgtatctcta aaccaggttt gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg
    39061 aacggtttag gtatcgcaat catttcgact tctgaaggtg ttgtcactga taaagaagct
    39121 cgtgaaaaaa atatcggcgg cgaagttatc gcttacgtat ggtaatcaag tagaatctac
    39181 aaggaggtgt ctttaagtga gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt
    39241 aacagttact caagacggaa acaacgttac agttaaagga ccaaaaggtg aattgactcg
    39301 tactttttca gcagatatca aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc
    39361 aaacgacagt aaagaaatga aaacaattca tggaacaact cgtgcgaact tcaacaacat
    39421 ggttgtaggt gtcagcgaag gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg
    39481 tgcacaatta caaggatcaa aacttgtatt gaacgttggt tactcacatc cagtagaaat
    39541 cacaccacca gctggagtaa ctgttgaagt accagctaac acacaagtaa ttgttaaagg
    39601 tgcgaacaaa gaagaagttg gcgaattagc tgcaaacatt cgtggaactc gtcctccaga
    39661 accttataaa ggtaaaggta ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa
    39721 aactggtaaa taatcgttta gaaaaattgc atagaagcca gcagcaggaa aaaggcattt
    39781 ggctgaagtt taccttcaaa aaaatacatt tttacttgtg ccgcagcttc tatttttcga
    39841 aacgtattta ctgcttgaaa tagtagtaca ataaaaataa agaggtgaca attgtgatta
    39901 caaaaccaga taagaacaaa acacgtcaaa agagacatcg tcgtgtacgt aacaatatct
    39961 ctggtactgc tgagcgccca cgcttgaacg tttttcgttc taacaaaaac atctacgctc
    40021 aaattattga tgacgtagcg ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa
    40081 tttcaggtgg aacaaaaaca gaagcagcac aagctgtcgg taaactagtt gctgaacgtg
    40141 cagctgaaaa aggcattaaa gtagtagtct ttgaccgtgg tggatacctt taccatggcc
    40201 gtgtgcaagc tttagctgaa gctgctcgcg aaaatggact agaattttag aagaaggagg
    40261 aataccattc atggtttata tcgatccaaa acatttggaa ttagaagacc gcgttgttgc
    40321 aattaaccgt gtaacaaaag ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt
    40381 tgttgtcggt gacaaaaacg gacacgtagg gtttggtaca ggtaaagctc aagaagttcc
    40441 tgaagcaatc cgtaaagcaa ttgaagatgc gaagaaaaac ttagttgaag tacctatggt
    40501 tggttcaaca atcccacacg aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa
    40561 acctgctgta gaaggttctg gggttgctgc tggtggacca gttcgtgccg tattagaatt
    40621 agctggggta gcagatatca cttctaaatc tttaggctca aatacaccaa tcaacgttgt
    40681 tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg
    40741 cggcaaatca gttgaagaat taatcggata aggaggacaa caataatggc tgaattaaaa
    40801 gttactttaa aacgcagtat tatcggacgt cctcaaaacc aacgcgatac agttaaagcg
    40861 ttaggtctta caaagattaa cagttcagtg gttaagcctt ctaatgaagc aattaaaggc
    40921 atgatcaata ctgtttctca cctagtggac gttgaagaaa tttaatttag aaaaatcata
    40981 actagaacat taaggaggtg ccaaacctat gaaacttcat gaattaaaac ctgctgaagg
    41041 ttcacgccaa gtacgtaacc gtgttggtcg tggtacttca tctggtaacg gaaaaactgc
    41101 cggacgcgga caaaaaggac aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga
    41161 agggggacaa acaccattgt tccgtcgttt accaaaacga ggattcacaa acgtgaaccg
    41221 taaagactat gcagtcatta atcttgatgt attgaaccgc tttgaagacg gagctgaagt
    41281 aactcctgtt gctttagttg aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt
    41341 attagctaat ggggaactaa acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc
    41401 agctcaagaa gcaattgaag cggctggtgg atcaatcgag gtgatctaat gtttagacta
    41461 ttaaaagatg cttttaaagt aaaagacatt agatcgaaaa tcctatttac tgtatttgtg
    41521 ttgtttgtat ttcgcctagg tgcacatatt actgtaccag gcgtaaatgc tgcttcattg
    41581 caaaaccttt cttctttgcc atttttcaac atgttgaact tggtaagtgg tagtgcgatg
    41641 caaaactttt ccattttctc aatgggggtt tcaccataca ttactgcttc tatcgtcatt
    41701 cagcttcttc aaatggacat cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt
    41761 ggtcgtaaaa aactgaatca agcgactcga tatctgacat tagtattggc atttatccaa
    41821 tctgtcggta tcactgctgg attcaatcaa tacgctcaat taggtttcgt gaataatcct
    41881 aacgtagcaa cttacgtaac gatcggtttg attctaacag ccggtacgat gtttgttact
    41941 tggctaggag aacagatcac agaaaaaggt atcggaaatg gcgtatcaat gatcattttt
    42001 gcaggtatca tttctagatt gcctgaaagt atcagagaaa tttatgaaga ttactttata
    42061 aatattgatc cttcagacat atggaaatct gctatttttg tggccatttt actagtagcg
    42121 gtcttgttga tcgtgacatt cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc
    42181 caatatacga aacgcgtagc tggcgcgcct acaagcagct atctgccgtt gaaagtaaat
    42241 gcagctggag ttattccagt tatctttgct agttcgttta ttgctacgcc aaatgctgtt
    42301 ttacaagcat tgagcggtag attcggttct gaaaactggt acaaagtgat gatggagatc
    42361 tttaactata atacagcacc tggtgctacg atttatacga ttctgatcgt tgccttcact
    42421 ttcttctacg cctttgttca agtaaaccct gagaaattag cggaaaactt gcaaaagcaa
    42481 ggaagttata taccaagcgt acgccctggt aaggggactg aagagtatgt ctcaagatta
    42541 ctgatgcgtt taagtacagt tggtgcagtc ttccttggtt tagtggcatt gcttccaatc
    42601 attgctcaaa tggtttggga tcttccacaa tcaatcggat taggcggtac aagcttactg
    42661 atcgttatcg gtgtagcttt agaaactgcg aaacagttag aaggcttaat gctgaaacgt
    42721 aaatatgttg gattcattga gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct
    42781 cacacattta ttaggaggga aaacaatgaa cctcatttta atggggctgc caggcgcagg
    42841 taaaggaaca caagctgaaa aaatcattga tgcttatggt attccgcaca tctcaacagg
    42901 agatatgttc cgcgcagcga tgcaaaacga aacagctctt ggcctggaag caaagtctta
    42961 tatggataaa ggtgcattag ttcctgatga agtaacaaat ggaatcgtga aagagcgact
    43021 agctgaacca gatacagaga aaggcttctt attagatggc tttccacgta cgctagatca
    43081 agcaaaagct ctggacgcaa tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga
    43141 tatccatgtt ggtgaagaaa tcttggtcga acgtctagcc ggccgattta tctgcagcaa
    43201 ttgtggagca acttaccata aaattttcaa ccctacaaaa gttgaagaca catgtgatcg
    43261 ttgcggtggg catgaattct atcaaagaga agatgataaa cctgagacgg ttaaaaatcg
    43321 tctggctatc aacatcaaaa atagtgaacc aatcttggct tattataaag aacaaggttt
    43381 gctaaacact attgatggtg ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat
    43441 cattgaaaaa tagcacgatt gcccgtagcc gacttgcatt atgcagttac tcttgtcaag
    43501 ttcaataaat tatgatagaa tatgtcagtc cgacttctag agaatcctct agatgatcca
    43561 ttttgagaat tttgaggtgg gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa
    43621 acaagtacaa ggaggtactg tgcgtggcaa aagaagatat gattgaagtc gaaggtacag
    43681 tcgtcgaaac tttgccgaat gcaatgttta aagttgaact agaaaacgga caccaagttc
    43741 ttgctactgt ttcaggtaaa attcgtatgc attacatccg tatcttacct ggtgacaaag
    43801 taacggttga attgtctcca tacgatctta accgtggccg tattacttat cgctttaaat
    43861 aattgtactc cgtaaaacta caggaggtat tatcatgaaa gtaagaccat cagtaaaacc
    43921 aatgtgcgaa cattgtaaag taattcgtcg taaaggacgc gttatggtga tttgcccagc
    43981 aaatccaaaa cataaacaac gtcaaggata attggaggtg ctataaatat ggctcgtatt
    44041 gcaggagtag atattcctcg tgataaacgt gtagtagttt cacttactta tatctatggt
    44101 attggtaaca ctactgctaa aaagatctta gctgatgctg gtgtatctga agatgttcgc
    44161 gttcgtgaat taacgaatga acaaacagat gctatccgtg cagaagtaga taaattaaaa
    44221 gttgaagggg atcttcgtcg tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt
    44281 tcttaccgtg gaatccgtca tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac
    44341 aatgcacgta ctcgtaaagg cccagctcgc gctatcgctg gcaagaaaaa ataattctta
    44401 agtgaaggag gttaaaactt catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag
    44461 aagaatatag aagcaggtat tgcacatatc cattctacat tcaataacac tatcatcatg
    44521 atcactgata ctcacggaaa tgcattagcg tggtcatcag ctggttcatt aggtttcaaa
    44581 ggaagcaaaa aatcaactcc ttttgccgct caaatggcag cagaaactgc tacaaaagca
    44641 gcaatggaac acggattaaa gactgttgaa gtaacagtta aaggacctgg ttctggacgt
    44701 gaagcagcaa ttcgttcatt acaagctaca ggtttagaag tgactgcaat tcgtgacgtg
    44761 actccagttc ctcataatgg tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg
    44821 ctcattttga gttttatcag taacgtcatt gaacaagaca cttttcgttt tgaaaggggt
    44881 aaagataaga atgattgagt tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga
    44941 ttatggcaag ttcatcgttg aacctcttga aagagggtat gggactactt tagggaattc
    45001 cctacgtcgt attttattat cttctttgcc aggtgctgcc atcactaata ttcaaatcga
    45061 tggtgtactg catgaattct ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat
    45121 cttgaacatt aaaggcttag cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat
    45181 cgatattacc ggacctgcta cagtaactgc tggcgatatt atcgttgata gtgatgtaga
    45241 aattcttaac aaagatatgt acatctgtag tgtctctgaa ggagctacgt tccatgctcg
    45301 cttaacagtg aaacctggac gtggttatgt tcaagcagat gaaaataaaa aagaagatat
    45361 gccaattggc gttcttccag tcgattcaat ctacacacct gttcgtcgtg tgaactatca
    45421 agttgagaac acacgtgttg gacaccgtga agattttgat aaattgacga tggagatttg
    45481 gacagatggt tcaattgaac cgttggaagc aatgagtttg tctgcgaaaa tcatgactga
    45541 acatttggat atttttgtga acttaactga tgaagcaaaa aatgctgaaa tcatggtcga
    45601 aaaagaagaa acacaaaaag aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc
    45661 tgttcgctct tacaattgtt tgaaacgtgc aggaatcaat actgtacaag aacttacaaa
    45721 taaatctgag cctgaaatga tcaaagtccg taacttagga cgtaaatcat tagaagaagt
    45781 taaagcaaaa ttgcacgact taggtttagg tttacgtaaa gaagattaaa accttttacg
    45841 aaggagggaa accaacgtga gttatcgtaa actaggacgc acatctagcc aacgtaaagc
    45901 gatgttgcgt gatttaacaa ctgatttatt aatcaacgaa cgtattgtga ctactgaagc
    45961 tcgtgcgaaa gaagttcgct caactgcaga aaaaatgatc actttaggta aacgcggaga
    46021 tttgcatgca cgtcgtcaag ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga
    46081 agaaaatgag gacatcgtca ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc
    46141 tcgttacgca gaacgtcaag gcggatacac acgcattttg aagacagaac caagacgcgg
    46201 tgatggtgca ccaatggttg tcttagaact tgtctaaaat cacactaata acatcacttt
    46261 atagatgatt cttttagagt gttatgatgt tggaagatat ttttccgagt ctagctcagc
    46321 gctgctcctc aagcgaatat cgcttgggga gtaagcactc atcataaagt gtttttttat
    46381 accttcaatt acctgtcatg acagatatgt ttgaaggcat aaaaaaacat tttaagcgag
    46441 aaataattga aaattttgtc tttttttgat agtgttagta ggaactttag gatgaagaag
    46501 gagtaatcat ggagccaatt attgaattag aaaagataaa ttataaatac cagccagatg
    46561 atcttcgccc cgcattgaaa gatgtctcct ttacaattga taaaggtgag tggatcgcta
    46621 ttattggaca caatggttca ggaaaatcga cacttgccaa aacgatcaat ggattgcttc
    46681 tgccagaatc agggatcgtg aaagtgggaa atcaaatatt ggatgaagaa aatatttgga
    46741 cgatccgaca gatggttgga atggtttttc aaaatccaga caatcagttt gttggttcga
    46801 cagtagaaga tgacgttgct tttggtcttg aaaatcaagg gattcccaga gaagaaatgc
    46861 tagtgcgtgt gaaagatgca cttgagaaag ttagaatggc tgagtttgct tcacgcgagc
    46921 ctgctcgttt atcaggaggg caaaaacaac gtgttgccat tgccggcgtg gttgccttac
    46981 gacctgatat cattatttta gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag
    47041 aagtgatctc tacgatcaag aagatcaaag aagaaagtca attgaccgtt atatctatca
    47101 ctcatgatat tgatgaagca gctaatgcca atcggatact agtgatgaga caaggcgaac
    47161 ttgtccgtga aggtacacca aaagaaattt tttctgctgg tcctgaactg atcgacttag
    47221 gtctagatct accatttcct gaaaaactga agagcgcact gaaagaacgt ggggttgatg
    47281 tgcccagtga atatatgaca gaagaaagga tggtggattg gttatggaca tccgttttga
    47341 acaagtagac ttcacttatc agccgaatac accatttgaa caaagagcct tatttgacat
    47401 caatatgacg atcaaagaaa acagttatac tgcattagta gggcataccg gaagcgggaa
    47461 atcaacctta cttcagcatt tgaatgcgct tgttaagcca acaagcggta cagtccatat
    47521 tggagaacga gatattcagc cggatacgga taataagaat ttaaagccca tccgaaaaaa
    47581 agttggcatc gtctttcagt ttccagaagc acagctattc gaagaaacgg tagcaaaaga
    47641 cattgctttc ggtcctaaaa actttggtgt cagcgaagaa gaagcactag tcctagcaaa
    47701 agaaacatta gaacaagttg ggctggatga aagctatttg gaacgttcgc catttgaact
    47761 ttcaggaggg caaatgcgta gagtagcaat cgctggtgtg cttgccatga ggccggaagt
    47821 acttgtattg gatgaaccaa cggcaggact tgatccccag ggcagaaaag aaatgatgga
    47881 gatgttttgg cgtcttcata aggaacagca gatcactatt gtattagtta cgcatttaat
    47941 ggatgatgta gccaattttg cagattatgt ctatgtgttg gaaaagggac gaatcgttaa
    48001 tagcggggag ccgcaagagg tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg
    48061 cgtacctact gccaccgagt ttgcagaaga attaatggct aaagggatga actttgctac
    48121 cttgcctttg acagctgaag aattagcaga cgctatcgtc cattatgctg gaggaaatgc
    48181 ccatgatgaa taaattgata tttggtcgtt atatgcctgg agattccttg atccatcgat
    48241 tggatccccg agctaaatta ttagccagtt tttattttat cggaatcatt tttttagcaa
    48301 ataattggga aacgtacctt ttgttggcat ttttcacatt actttctgtc ttcttgtcaa
    48361 aagtagattt gaaatttttt atccgaggca ttcgcccatt gatctggcta atcctcttca
    48421 ctgtagcttt gcaggtcttg tttacaagtg gcggagaaac ttattggagt tggggtgttt
    48481 tccatatcac tgaatttggt gtgatgaacg gcctctttat tttctgtcga ttcgtgttga
    48541 tcatttttat gtcgactcta ctcacgctta ctacaccgcc tttggaatta tcggatgcga
    48601 ttgagtactt gcttcgtcca ttgaaagccg tacggttccc agtccacgaa atttcattga
    48661 tgctgtcgat tgctcttcgt tttgtgccta cattgatgga cgaaacagaa aagatcatga
    48721 acgctcagcg cgctcgcggt gttgattttg gtgaaggaaa cctagttcag aaaatgaaag
    48781 cagtcattcc tttgctgatt cctttatttg tcagcagctt caaccgtgcg gaagatcttg
    48841 cgactgctat ggaagcaaga ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat
    48901 tgcattggca taatagagac acgatggtta tcattgcttt tgtattatta actgtgctac
    48961 tgatcttttt aagaggataa acgttcagtg ggccgctatc tattcttttc ttgctgatct
    49021 gtttgcaaga aaggatgcaa gcagtgtatg acaagaaata ttagggtctg tggcagacta
    49081 aggtcacagg cctctttctt ttatctgaaa agaaagaacg aaaaa
//
LOCUS       DAYYBG010000013        48651 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000013 DAYYBG010000000
VERSION     DAYYBG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.013"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..792)
                     /locus_tag="ACHAUN_000729"
     CDS             complement(<1..792)
                     /locus_tag="ACHAUN_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8459454.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKR"
     gene            complement(765..1445)
                     /locus_tag="ACHAUN_000730"
     CDS             complement(765..1445)
                     /locus_tag="ACHAUN_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8459455.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1468..2406)
                     /locus_tag="ACHAUN_000731"
     CDS             complement(1468..2406)
                     /locus_tag="ACHAUN_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8459456.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2651..3502)
                     /gene="ispE"
                     /locus_tag="ACHAUN_000732"
     CDS             complement(2651..3502)
                     /gene="ispE"
                     /locus_tag="ACHAUN_000732"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8459457.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3656..6229)
                     /gene="mprF"
                     /locus_tag="ACHAUN_000733"
     CDS             complement(3656..6229)
                     /gene="mprF"
                     /locus_tag="ACHAUN_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8459458.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6370..7176
                     /gene="proC"
                     /locus_tag="ACHAUN_000734"
     CDS             6370..7176
                     /gene="proC"
                     /locus_tag="ACHAUN_000734"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8459459.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7255..8811)
                     /gene="rny"
                     /locus_tag="ACHAUN_000735"
     CDS             complement(7255..8811)
                     /gene="rny"
                     /locus_tag="ACHAUN_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8459460.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9222..10271)
                     /gene="recA"
                     /locus_tag="ACHAUN_000736"
     CDS             complement(9222..10271)
                     /gene="recA"
                     /locus_tag="ACHAUN_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8459461.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10380..11621)
                     /locus_tag="ACHAUN_000737"
     CDS             complement(10380..11621)
                     /locus_tag="ACHAUN_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8459462.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11861..12439)
                     /gene="pgsA"
                     /locus_tag="ACHAUN_000738"
     CDS             complement(11861..12439)
                     /gene="pgsA"
                     /locus_tag="ACHAUN_000738"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8459463.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12648..13571)
                     /locus_tag="ACHAUN_000739"
     CDS             complement(12648..13571)
                     /locus_tag="ACHAUN_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8459464.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14310..15596)
                     /locus_tag="ACHAUN_000740"
     CDS             complement(14310..15596)
                     /locus_tag="ACHAUN_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8459465.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15589..16857)
                     /locus_tag="ACHAUN_000741"
     CDS             complement(15589..16857)
                     /locus_tag="ACHAUN_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8459466.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17109..17570
                     /gene="mscL"
                     /locus_tag="ACHAUN_000742"
     CDS             17109..17570
                     /gene="mscL"
                     /locus_tag="ACHAUN_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8459467.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17629..18075)
                     /locus_tag="ACHAUN_000743"
     CDS             complement(17629..18075)
                     /locus_tag="ACHAUN_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8459468.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18168..18413)
                     /locus_tag="ACHAUN_000744"
     CDS             complement(18168..18413)
                     /locus_tag="ACHAUN_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8459469.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18528..19106)
                     /locus_tag="ACHAUN_000745"
     CDS             complement(18528..19106)
                     /locus_tag="ACHAUN_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8459470.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19183..19713)
                     /locus_tag="ACHAUN_000746"
     CDS             complement(19183..19713)
                     /locus_tag="ACHAUN_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8459471.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19717..20577)
                     /gene="rlmB"
                     /locus_tag="ACHAUN_000747"
     CDS             complement(19717..20577)
                     /gene="rlmB"
                     /locus_tag="ACHAUN_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8459472.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20570..20965)
                     /locus_tag="ACHAUN_000748"
     CDS             complement(20570..20965)
                     /locus_tag="ACHAUN_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8459473.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(20962..22374)
                     /gene="cysS"
                     /locus_tag="ACHAUN_000749"
     CDS             complement(20962..22374)
                     /gene="cysS"
                     /locus_tag="ACHAUN_000749"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8459474.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22371..22907)
                     /gene="epsC"
                     /locus_tag="ACHAUN_000750"
     CDS             complement(22371..22907)
                     /gene="epsC"
                     /locus_tag="ACHAUN_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8459475.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(22993..23232)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23229..24689)
                     /gene="gltX"
                     /locus_tag="ACHAUN_000751"
     CDS             complement(23229..24689)
                     /gene="gltX"
                     /locus_tag="ACHAUN_000751"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8459476.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24859..26016)
                     /locus_tag="ACHAUN_000752"
     CDS             complement(24859..26016)
                     /locus_tag="ACHAUN_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8459477.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26050..27420)
                     /gene="radA"
                     /locus_tag="ACHAUN_000753"
     CDS             complement(26050..27420)
                     /gene="radA"
                     /locus_tag="ACHAUN_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8459478.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27483..28010)
                     /locus_tag="ACHAUN_000754"
     CDS             complement(27483..28010)
                     /locus_tag="ACHAUN_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8459479.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28240..29046
                     /gene="proB"
                     /locus_tag="ACHAUN_000755"
     CDS             28240..29046
                     /gene="proB"
                     /locus_tag="ACHAUN_000755"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8459480.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29043..30290
                     /locus_tag="ACHAUN_000756"
     CDS             29043..30290
                     /locus_tag="ACHAUN_000756"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8459481.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30366..30710
                     /locus_tag="ACHAUN_000757"
     CDS             30366..30710
                     /locus_tag="ACHAUN_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459482.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30827..31153)
                     /locus_tag="ACHAUN_000758"
     CDS             complement(30827..31153)
                     /locus_tag="ACHAUN_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459483.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31768..32970)
                     /locus_tag="ACHAUN_000759"
     CDS             complement(31768..32970)
                     /locus_tag="ACHAUN_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8459484.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33087..33602
                     /locus_tag="ACHAUN_000760"
     CDS             33087..33602
                     /locus_tag="ACHAUN_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8459485.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33801..34247)
                     /locus_tag="ACHAUN_000761"
     CDS             complement(33801..34247)
                     /locus_tag="ACHAUN_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8459486.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34294..35772)
                     /gene="msr(C)"
                     /locus_tag="ACHAUN_000762"
     CDS             complement(34294..35772)
                     /gene="msr(C)"
                     /locus_tag="ACHAUN_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8459487.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36126..36755)
                     /locus_tag="ACHAUN_000763"
     CDS             complement(36126..36755)
                     /locus_tag="ACHAUN_000763"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8459488.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36739..37203)
                     /locus_tag="ACHAUN_000764"
     CDS             complement(36739..37203)
                     /locus_tag="ACHAUN_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8459489.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37690..38958)
                     /locus_tag="ACHAUN_000765"
     CDS             complement(37690..38958)
                     /locus_tag="ACHAUN_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8459490.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39189..39926)
                     /locus_tag="ACHAUN_000766"
     CDS             complement(39189..39926)
                     /locus_tag="ACHAUN_000766"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8459491.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(39993..40562)
                     /locus_tag="ACHAUN_000767"
     CDS             complement(39993..40562)
                     /locus_tag="ACHAUN_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8459492.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40690..41562
                     /locus_tag="ACHAUN_000768"
     CDS             40690..41562
                     /locus_tag="ACHAUN_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8459493.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            41904..42776
                     /locus_tag="ACHAUN_000769"
     CDS             41904..42776
                     /locus_tag="ACHAUN_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8459494.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            42773..44731
                     /gene="nagE"
                     /locus_tag="ACHAUN_000770"
     CDS             42773..44731
                     /gene="nagE"
                     /locus_tag="ACHAUN_000770"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8459495.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(45063..45914)
                     /locus_tag="ACHAUN_000771"
     CDS             complement(45063..45914)
                     /locus_tag="ACHAUN_000771"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8459496.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            46037..46504
                     /locus_tag="ACHAUN_000772"
     CDS             46037..46504
                     /locus_tag="ACHAUN_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8459497.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(46767..47249)
                     /locus_tag="ACHAUN_000773"
     CDS             complement(46767..47249)
                     /locus_tag="ACHAUN_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8459498.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(47349..48212)
                     /locus_tag="ACHAUN_000774"
     CDS             complement(47349..48212)
                     /locus_tag="ACHAUN_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8459499.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        13907 a        10423 c         8686 g        15635 t
ORIGIN      
        1 tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga ttgttgctcc
       61 tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta gaaatcctac
      121 acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac cgattgctgc
      181 gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca tcacagcaat
      241 agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta gcccatccac
      301 gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata aaaagaacaa
      361 tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg taatggtcac
      421 gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc tcaaattcat
      481 caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga tttctgaata
      541 ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa cgactagcat
      601 ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg ctaaagagac
      661 atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca cacctagcat
      721 cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga attcatatga
      781 aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc cgcatactgt
      841 ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac atgcgcacta
      901 tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt tgttggttca
      961 tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat tcgctgcttt
     1021 tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc tacagcttct
     1081 aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca gcggcctctt
     1141 ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc attgaaagat
     1201 gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt tttctcagca
     1261 atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac caaggttgac
     1321 ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac atgataagag
     1381 acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat ttcgatataa
     1441 cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag ctttcagatt
     1501 tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag tcaagctttc
     1561 taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct cagctacctt
     1621 cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca caaattcttt
     1681 tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg aaatcgctac
     1741 ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag tgacaaatgt
     1801 gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt caagcttttt
     1861 gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt tttcgcctaa
     1921 ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc aaacatgtgg
     1981 atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt ctgcttcttg
     2041 cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt tgctatccac
     2101 tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca cgaaaacatc
     2161 tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg gctccgttcc
     2221 agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg taaaatcata
     2281 catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt ccttgttcgt
     2341 atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca aaagatattt
     2401 tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat taacgtgaac
     2461 taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg atttaagcat
     2521 gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag ccatttcact
     2581 acaataaaaa actgaagaat accattttag gattcctcag tttttcatca cctattttac
     2641 ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa ccttttaacc
     2701 cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg gttggtccgc
     2761 ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg atttgatgga
     2821 tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt ttgatcatct
     2881 gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga tgttggatgg
     2941 cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt ggtttgacca
     3001 acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct ttgccaccga
     3061 caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc aaaccgattt
     3121 ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct cttagagcag
     3181 ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc ttcttaacaa
     3241 agatcttgac accggttttg atattgtatt tattttttag caataaagca gcttgataaa
     3301 cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg attttgtctt
     3361 ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc accatttcca
     3421 actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt atcttggctg
     3481 gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc gcaagtattc
     3541 tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt ttgtcatcgt
     3601 ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt gattcttata
     3661 attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt cctatcagac
     3721 tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca aaataacgtc
     3781 cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag gaatagaaaa
     3841 aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa aaatctgttt
     3901 ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc attttctcgt
     3961 atacaataaa cagattggct aacagatagt cagctaattc ctcagattct ttaggtaaga
     4021 cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg atgaagccaa
     4081 cgactttttg atctttacgg acaataccga caccgctaga taatacatat gatttatcaa
     4141 atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt gaaacttcat
     4201 ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg tatccttctt
     4261 tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct gttgctagat
     4321 caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga agcatcacta
     4381 cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct gcttgtttca
     4441 taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct cctaaaacaa
     4501 agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg tcttcttggt
     4561 aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg cctccgtatg
     4621 tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc gtagtatctt
     4681 cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa ccaataagcc
     4741 ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt attcgaccga
     4801 ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa aagccgaaca
     4861 agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga taaaattctt
     4921 ttctggataa ccacacagct aacagaatca agacatatac caatatcaac tgccaagata
     4981 acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta ggcagaaatg
     5041 cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag ccaaccaata
     5101 gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga aatagccgac
     5161 tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc gcaaaataga
     5221 cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga atgttatcta
     5281 aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc gtcataaaag
     5341 gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca tcttgccgaa
     5401 cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt ccacctggga
     5461 ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc gaaagcatcg
     5521 aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct cccgtccatt
     5581 gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg aagaacaatg
     5641 tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa
     5701 gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca tcaataaatg
     5761 taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt gatacagtgg
     5821 ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga cttgctccta
     5881 ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca gcaagaagaa
     5941 actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc gtcacccaat
     6001 catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc aggatcgtca
     6061 tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca ttagcaatat
     6121 ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt tttaaaatca
     6181 gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca ttttcccatt
     6241 cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt aatttttcca
     6301 ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga
     6361 gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc tattattgat
     6421 ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag tggacatttt
     6481 gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag taatcaggaa
     6541 gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat tgaaaaagta
     6601 ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc ttcaggaaaa
     6661 tcattaatag atttagaaac agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg
     6721 ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc
     6781 acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag cgtctatcct
     6841 ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc cgcattcgtt
     6901 tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc caaacagaca
     6961 gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct ttcaagtgat
     7021 aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac tgttgcaggt
     7081 gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat caatacgacg
     7141 atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt tttcgtgcct
     7201 tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt tttattattt
     7261 agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc caggataatc
     7321 cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt ctaaatccga
     7381 gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga
     7441 ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac ggatgtagtt
     7501 ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg ctgcagctac
     7561 aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga tcgcgttgat
     7621 tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa cgtgagatcc
     7681 ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac gttttgctaa
     7741 ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa cctcgaccga
     7801 atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga tgatcttgat
     7861 tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg
     7921 aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac gcgctggatg
     7981 gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc ttcggattgg
     8041 atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat cgataccagt
     8101 cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac ctttcatttc
     8161 atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg
     8221 ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt tggcacgctg
     8281 ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag tcgatttcat
     8341 gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt ctttttgttg
     8401 atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct gttttgaact
     8461 aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt catctttacg
     8521 attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt ctaacttact
     8581 ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct tagcttctaa
     8641 caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga ttccagcagc
     8701 tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca taaaccctaa
     8761 accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca taaaatccac
     8821 ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa ttattctaaa
     8881 ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact ttattctaat
     8941 gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta cttattcatt
     9001 aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt taaattgaga
     9061 aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta tgtacccact
     9121 cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag
     9181 tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc
     9241 aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct
     9301 gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta
     9361 gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc accgctttta
     9421 tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa
     9481 ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact
     9541 ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt
     9601 acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga
     9661 ttcgcaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt
     9721 gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac
     9781 ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact
     9841 gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga
     9901 ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt
     9961 tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt
    10021 ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca
    10081 tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca
    10141 agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata
    10201 gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga
    10261 tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc
    10321 ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat
    10381 cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg
    10441 atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa
    10501 atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg
    10561 gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc
    10621 acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa
    10681 ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca
    10741 gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca
    10801 gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg
    10861 tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct
    10921 tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt
    10981 ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg
    11041 accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca
    11101 gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca
    11161 ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa
    11221 gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga
    11281 ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct
    11341 tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca
    11401 tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga
    11461 gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg
    11521 atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct
    11581 agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata
    11641 tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa
    11701 aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat
    11761 aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag
    11821 agttagacgg caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca
    11881 caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat
    11941 aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca
    12001 gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca
    12061 tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga
    12121 caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag
    12181 tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc
    12241 ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa
    12301 ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat
    12361 ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta
    12421 atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc
    12481 gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat
    12541 tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt
    12601 cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat
    12661 tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg
    12721 gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt
    12781 gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa
    12841 acataggcat tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg
    12901 gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct
    12961 gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac
    13021 tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta
    13081 agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag
    13141 cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc
    13201 caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt
    13261 tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg
    13321 actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg
    13381 taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt
    13441 tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc
    13501 cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt
    13561 ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca
    13621 tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc ggtcacaagt
    13681 cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg actcggagtt
    13741 aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta
    13801 gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta atactcaggt
    13861 caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
    13921 ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt tcttattact
    13981 tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag tagcttcttc
    14041 ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    14101 tcttgaagct gaccacttct gtcacaacct ctttgattac ttcgtttgat tttattttaa
    14161 tataagttga ttaaaagaga gcatgatttg ttttattttc tcactaattt ttccaacaca
    14221 actaagaata ccataaaagc tgattttgga ggaggttcct actacaagtc taaaacgaaa
    14281 tttaagactt ttcctcataa aatactggtt tattttggtc tcatgtaaaa ctcgctgaag
    14341 gcctcttcac ttataaatgc ttccccagct gccagtacgt ctttcatttg gatcgaatcg
    14401 atgatttcag gaagatcaaa taaagttcga tcgccaaata aactttgcgt aaattgattg
    14461 gcaatatatt cgatcgaatt taatgattgg aaatattgtc caagcatttt cttcttcagc
    14521 aaatcgagat ttgtttctga cacttccgga tcatcagcga atccaagaat gatttctttg
    14581 actttttctg ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat
    14641 tcacgatcca agctgaattc aaaaccaaag ctgtcatcga taattccttg attgtacatc
    14701 gctagatagt ttcgagaagt attgcctaaa atcatttgga acaacagatt gattgctgtt
    14761 ttatagcgta acagttctgt tccttcttgc ggcaatgtgt ctaatccttt aataccaagt
    14821 acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg
    14881 ttttcaggga aataacgtac aatttcttgc tttggaggga aattttttgc ttcttggttt
    14941 tcccgaataa gtttcatcaa tttctcaggc tccatcttac caactacaaa taaaaccata
    15001 ttgcttggct gataaaacgt ccgatagcac gtatacaagt cttgagctgt gattttgtcg
    15061 atactttcaa ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata caagttattt
    15121 agtatgccaa aaaacatccg ccagttaggg tcgtcttcat acatttggat ttcctgaccg
    15181 atgattcctt tttccttgtt tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg
    15241 atcagagtcg tcagattctt ttctacctga tccgtcgtag aaaacagata gctggttttc
    15301 gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc caaataattg aaagacatca
    15361 ccatcttctt tttcaaacaa cttatgttct aaaaaatggg caattccatc tggaactttc
    15421 accttttctt tttcaccata gggaataaat tcgttatcga tcgaaccata attcgtactg
    15481 aacaaaccat atgtcttgtg atagtcattt ttaggcaaca aatacacggt caaaccgttt
    15541 ggcagtactt catgataaag tgtttcattg atttgctcat attctgtttt attcatttgc
    15601 atttcctcct gctaaaaaga agattgcctg caattcgatt tgctcagcta cttgttgtat
    15661 ctctttgatc gttacgctct ccatacgttt caaccattct tcatcagata attttgtttc
    15721 cggcagccag ctgtccaaaa aagaagcttc gattgccgct tgcggagagt ctagagaaag
    15781 cagatattgg tttctcagca ttgcttttgt ctgagacagt tccaactctg ttatttcacc
    15841 atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt acttgatttc ggttcttttc
    15901 atcgataccg gtttggacac tcatgaatcc tcggaacgta tccacgctac tagaggcata
    15961 ataagctaga ctttctttct ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc
    16021 aaataaaccg ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat acacatttgt
    16081 ctgataagct aggttcagct ttgcttgaac gattggctca cgtacttgtc gttccttcac
    16141 gatattcact tgaggttggg tataaaacag atctggataa actgtttccc gcgactcaaa
    16201 tggtaattgc ccaactgctt cagctgcttt gtccggatca acatcgccga caacaaaaat
    16261 gtcgatttga tcatttgcca tcatttcttg atagtaggac gctagcgaac tagcagtgat
    16321 cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa
    16381 ataaagttct tgcaaggcat aggaagcaaa agtttgttta tcttccttca aactttccaa
    16441 atatagacgc agattgttct tttccaagtc aaacgtcaac tgatcaaact gatgattttt
    16501 aatatttgga taaaataaaa cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg
    16561 atcattgaca tattcaccat tcacaaaact gatccctgcg tttagccaat gtaaatttcc
    16621 tttttttcta actgataatc caaagcttgc gccataaagc tctgcaagtt gcgcacttaa
    16681 ctttgtctga tccgaataat tcaaactatt tgtttccaac atgctgctga gaagtgaacg
    16741 tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt
    16801 gtattgtttt gttggtaaga catgaagatt cacgccttct gccaattgat acaccatatt
    16861 tatatgctcc ttttcacagt tgattgcccg cttactcttt taatgggtca acctgccttt
    16921 tttacttatt cctctgcttg aaatttataa gttcaggcaa ttcgccgttt tttctaaatg
    16981 atgttacact ttatagtata gcatagatgt tttttatcaa aagaaacaga cggctaaaag
    17041 cgtatattgt ttttttattt tttatttttc gatatactaa agacaaataa aagaagggag
    17101 ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg
    17161 ctgtcggcgt tgttattggt agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct
    17221 tgatcacacc actaataggt tggtttgtcg caatgatcac aggtacttct gatttagaag
    17281 gagcactttc aattcttgac tggtcgccga caaaaggcgt cacatttgcg tttggtaacg
    17341 ttattagtgc tatcattaca tttctgatca ctggttttgt cttattctta gttgtcaaag
    17401 cggcaaaccg tgcgaagaat ttacgctcca aagaagagca agtggaagtg gaagaaactc
    17461 ctactgcaga agattatctt agtgacatac gcgatcttct tgccaaacaa gtggcagaag
    17521 acaatacaca tccagtagag caaacaaatc tttccgatac ttctaaataa taaagaatct
    17581 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    17641 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    17701 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    17761 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    17821 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    17881 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    17941 agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    18001 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    18061 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    18121 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    18181 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    18241 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    18301 aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    18361 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    18421 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca taaaaaaaaa
    18481 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    18541 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    18601 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    18661 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    18721 aataaaagat gctgaattgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    18781 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    18841 gcgttctgct tcctcaacag agaatagatt tgattgctta taacgctttt ataataactt
    18901 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    18961 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    19021 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    19081 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    19141 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    19201 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    19261 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    19321 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    19381 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    19441 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    19501 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    19561 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    19621 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    19681 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    19741 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    19801 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    19861 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    19921 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    19981 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    20041 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    20101 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    20161 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    20221 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    20281 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    20341 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    20401 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    20461 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    20521 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    20581 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    20641 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    20701 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    20761 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    20821 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    20881 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    20941 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    21001 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    21061 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    21121 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    21181 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    21241 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    21301 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    21361 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    21421 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    21481 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    21541 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    21601 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    21661 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    21721 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    21781 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    21841 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    21901 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    21961 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    22021 catatgatcc atgactcttg gatgcagtgt agcaggttcg acattcaacg ctttcgtatc
    22081 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    22141 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    22201 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    22261 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    22321 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    22381 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    22441 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    22501 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    22561 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    22621 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    22681 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    22741 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    22801 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    22861 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    22921 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    22981 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    23041 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    23101 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    23161 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    23221 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    23281 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    23341 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    23401 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    23461 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    23521 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    23581 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    23641 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    23701 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    23761 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    23821 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    23881 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    23941 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    24001 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    24061 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    24121 cgtttttgga tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    24181 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    24241 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    24301 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    24361 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    24421 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    24481 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    24541 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    24601 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    24661 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    24721 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    24781 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    24841 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    24901 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    24961 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    25021 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    25081 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    25141 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    25201 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    25261 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    25321 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    25381 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    25441 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    25501 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    25561 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    25621 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    25681 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    25741 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    25801 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    25861 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    25921 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    25981 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    26041 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    26101 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    26161 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    26221 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    26281 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    26341 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    26401 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    26461 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    26521 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    26581 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    26641 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    26701 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    26761 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cggttttgag
    26821 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    26881 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    26941 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    27001 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    27061 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    27121 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    27181 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    27241 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    27301 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    27361 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    27421 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    27481 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    27541 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    27601 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    27661 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    27721 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    27781 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    27841 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    27901 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    27961 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    28021 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    28081 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    28141 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    28201 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    28261 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    28321 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    28381 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    28441 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    28501 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    28561 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    28621 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    28681 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    28741 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    28801 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    28861 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    28921 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    28981 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    29041 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    29101 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    29161 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    29221 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    29281 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    29341 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca
    29401 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    29461 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    29521 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    29581 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    29641 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    29701 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    29761 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    29821 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    29881 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    29941 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    30001 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    30061 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    30121 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    30181 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    30241 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    30301 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    30361 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    30421 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    30481 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    30541 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    30601 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    30661 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    30721 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    30781 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    30841 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    30901 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    30961 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    31021 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    31081 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    31141 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    31201 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    31261 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    31321 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    31381 cagtctcaca acctcttggt aaacggcgtt caatcagctg accctcggta ttagctcaaa
    31441 aaacggtaaa atatgagaag ctatttttcc gttttgtttc tctaatactt aggataaaac
    31501 gctgatttta caacctctgt taaacggtgt tcaatcagct gtccttcggt attagctcaa
    31561 aaaacggcaa aatatgagga actatttttc cgtttgtttc tctaatactc aggacaaaac
    31621 gctgatttta caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    31681 caaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag
    31741 gacagctttt tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg
    31801 gtgataaatg atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata
    31861 gctttcacca atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa
    31921 aaagcggaca aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat
    31981 agcaatcgtc gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct
    32041 gatcggtaca aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa
    32101 taagacaaat gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc
    32161 aacgaaaatc gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc
    32221 taattcagaa tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa
    32281 attatataac aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac
    32341 accttcccaa aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag
    32401 ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc
    32461 actgcccaag accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc
    32521 atacatcatg attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc
    32581 agtagagaca aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc
    32641 gatattccca gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat
    32701 caatgttttt ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc
    32761 agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg
    32821 tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca
    32881 ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg
    32941 tatatctatt tgattctcca atatttccat ccctttctct ctattagcat catacatgat
    33001 ttattttaca tgtcaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag
    33061 aaaataagac gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc
    33121 ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag
    33181 cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg
    33241 ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg
    33301 ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc
    33361 tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca
    33421 ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct
    33481 cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt
    33541 gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt
    33601 aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa
    33661 gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg
    33721 atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt
    33781 tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa
    33841 agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca
    33901 ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa
    33961 gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc
    34021 ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg
    34081 aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat
    34141 aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa
    34201 cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc
    34261 tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt
    34321 tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc
    34381 atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat
    34441 cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt
    34501 aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt
    34561 cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga
    34621 aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc
    34681 cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt
    34741 gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt
    34801 tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa
    34861 ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg
    34921 aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct
    34981 ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga
    35041 agacaaccga tccagtctga tttgttgctt tttcttttta ctggacactt tttccaactt
    35101 ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat
    35161 cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact
    35221 ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag
    35281 ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag
    35341 ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag
    35401 catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc
    35461 gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa
    35521 atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc
    35581 tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga
    35641 tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga
    35701 atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc
    35761 taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa
    35821 aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga
    35881 aataactatg actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga
    35941 aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta
    36001 gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt
    36061 ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa
    36121 accttttatt cgaacatagt gatcttttct ggtgctaaac gaacagactc gtaaccttct
    36181 tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt atccaaacca
    36241 aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga
    36301 tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg
    36361 ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct
    36421 ctgacttctt ttggcatctt ccctgattcg taagcttggt tgtagatttc ttcgccatat
    36481 tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc
    36541 gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca
    36601 aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc
    36661 agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca
    36721 gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt
    36781 tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc
    36841 tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg
    36901 cttgattttc ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg
    36961 atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc
    37021 tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt
    37081 cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt caaagatctg
    37141 gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga
    37201 cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata
    37261 aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag
    37321 tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc
    37381 taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg cagtagataa
    37441 ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa
    37501 attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt
    37561 ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa
    37621 acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac
    37681 tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag
    37741 ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt
    37801 aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca
    37861 accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact
    37921 gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc
    37981 ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca atgtatataa
    38041 atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt
    38101 atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg
    38161 cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct
    38221 tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt
    38281 tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt
    38341 aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc cattatagct
    38401 tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc
    38461 actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa
    38521 actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg
    38581 agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg
    38641 tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt
    38701 ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg
    38761 ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac tttctgtatc
    38821 gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc
    38881 ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa
    38941 taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag
    39001 tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt
    39061 aataaatttg ctttttctgc ataaaaagac gtaaatgaag aatattcttc tccattcacg
    39121 ccttaaattg atggtcgttt gaataactat ttaacaagta attctctctg gatattatcc
    39181 gttatggatt atacttcctg tgtcgtcgga cggataatca tgttgctgac agacatccgg
    39241 tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcagatgt taatccccat
    39301 tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat
    39361 tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct
    39421 tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa
    39481 tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt
    39541 ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca
    39601 agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct
    39661 gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct
    39721 tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa
    39781 gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct
    39841 ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca
    39901 atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct
    39961 tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg
    40021 attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca
    40081 ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct
    40141 ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt
    40201 ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct
    40261 tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc
    40321 cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca
    40381 tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac
    40441 ccagtattca gcgctgctaa aggctcttcg ttttccgctt ggatctgatg gatcacttca
    40501 aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc
    40561 attttgctcc ctccttttat ccttacttta taacggacgg cttatgatgt ctaatactta
    40621 taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg
    40681 agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat
    40741 atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa
    40801 gatttagaag aagaacttgg tgtgacactt ttcactcgag gctcccgaga gatacagttg
    40861 acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa
    40921 acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc
    40981 gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct
    41041 gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc
    41101 ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt
    41161 cgactgccag cccaagatcg ttggggactt ttgatacatc ggaatcatcc tttagcaaag
    41221 caaacagtca tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg
    41281 tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc
    41341 ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca
    41401 ttagctctag atgggatcat caatacttat gggacggaaa aagtattcat cccactcgat
    41461 ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct
    41521 gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc
    41581 gctttcagct ttttaacaac gaataagtta agcaaaagat ccaatgatta aataacataa
    41641 gtttcttaat ttgttccttc cttttatttc tgataattcc agtaaattac tataattata
    41701 aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg
    41761 tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg
    41821 aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc
    41881 aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat
    41941 tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa
    42001 aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag
    42061 gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag
    42121 aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga
    42181 tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc
    42241 gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat
    42301 gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat
    42361 atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc
    42421 gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata
    42481 tccattcaga accattttca ctgaattatt caagactggt gaatcattta cgactgcttc
    42541 tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca
    42601 aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag
    42661 aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt
    42721 taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc
    42781 atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc
    42841 tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt
    42901 tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt
    42961 agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt
    43021 tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc
    43081 agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg
    43141 attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc
    43201 tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag
    43261 tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt
    43321 cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat
    43381 ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa
    43441 tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc
    43501 tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa
    43561 tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ttagcagcag cttttgcttc
    43621 attctttact ggtgtaacag aaccacttga attctcattt atgttcgttg cttggccatt
    43681 atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg
    43741 gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc
    43801 gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta
    43861 ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc
    43921 agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc
    43981 aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac
    44041 tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc
    44101 cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac
    44161 agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg caacacgacc
    44221 tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga
    44281 tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt
    44341 cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc
    44401 tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac
    44461 agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga
    44521 accattcact ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat tgattgcttt
    44581 agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac
    44641 aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga
    44701 taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg
    44761 ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg
    44821 gcgttctcct gccaactccg cggtaataag tccagccaaa cgtaacatat gaagagctat
    44881 tttttatttg actcttctta ttacttgatg cagaacagct tttttacaac ctctgataaa
    44941 cggtgttcaa aaaccagttt atcggtatga agtcaaattt atgcaaaaat gtggaaagcc
    45001 attttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata
    45061 cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc
    45121 aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg
    45181 atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca
    45241 aacgctgcat cacttgcttc aataatttct aactttttac ctgtcgcacg ctctaatgcc
    45301 ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca
    45361 ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatac ccaacctgtt
    45421 tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt
    45481 aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta
    45541 atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct
    45601 gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc
    45661 ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct
    45721 ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca
    45781 gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt
    45841 ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt
    45901 actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta
    45961 catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat
    46021 gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc
    46081 ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat
    46141 cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat
    46201 ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt
    46261 actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac
    46321 aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg
    46381 gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa
    46441 aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt
    46501 ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta
    46561 ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag
    46621 cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc
    46681 tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa
    46741 gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct
    46801 cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag
    46861 gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt
    46921 aattcttcag gcgattctgc taaagcgatt acaacaagat acgttccttt tgggaatggg
    46981 atgattcggg tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt
    47041 ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat
    47101 gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa
    47161 ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct
    47221 ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca
    47281 tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta
    47341 ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa
    47401 tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaactcata atctgaagaa
    47461 ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct
    47521 ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac
    47581 ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag
    47641 cgggctccat caaatccata agaaacgttg attacttgat ttggatataa atctcctaac
    47701 ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga
    47761 ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct
    47821 ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag
    47881 cgtggaaaag atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa
    47941 tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct
    48001 gtcactcttg atccatctag gagatcgcga ttagccaaag ccaatctacg gtttttgata
    48061 tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag
    48121 attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg
    48181 taatcgatca attggttcag atctttaagc atatttatct tcctccttgc ttgccttttt
    48241 atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta
    48301 gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt
    48361 attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag acacattcta
    48421 agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg
    48481 tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga
    48541 tacatcatac ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt
    48601 tttgtcagat aaataaaaaa caaaaaaagt tccatcttta aaacacgcta t
//
LOCUS       DAYYBG010000014        44908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000014 DAYYBG010000000
VERSION     DAYYBG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.014"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1054)
                     /locus_tag="ACHAUN_000775"
     CDS             complement(44..1054)
                     /locus_tag="ACHAUN_000775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8459500.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1234..1578)
                     /locus_tag="ACHAUN_000776"
     CDS             complement(1234..1578)
                     /locus_tag="ACHAUN_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8459501.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1571..2005)
                     /locus_tag="ACHAUN_000777"
     CDS             complement(1571..2005)
                     /locus_tag="ACHAUN_000777"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8459502.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2143..2880
                     /locus_tag="ACHAUN_000778"
     CDS             2143..2880
                     /locus_tag="ACHAUN_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459503.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2873..4084
                     /locus_tag="ACHAUN_000779"
     CDS             2873..4084
                     /locus_tag="ACHAUN_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459504.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4288..5070
                     /locus_tag="ACHAUN_000780"
     CDS             4288..5070
                     /locus_tag="ACHAUN_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8459505.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5140..5802
                     /gene="trmB"
                     /locus_tag="ACHAUN_000781"
     CDS             5140..5802
                     /gene="trmB"
                     /locus_tag="ACHAUN_000781"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8459506.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5900..7654)
                     /gene="ade"
                     /locus_tag="ACHAUN_000782"
     CDS             complement(5900..7654)
                     /gene="ade"
                     /locus_tag="ACHAUN_000782"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8459507.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7786..8715
                     /gene="comGA"
                     /locus_tag="ACHAUN_000783"
     CDS             7786..8715
                     /gene="comGA"
                     /locus_tag="ACHAUN_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8459508.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8672..9721
                     /gene="comGB"
                     /locus_tag="ACHAUN_000784"
     CDS             8672..9721
                     /gene="comGB"
                     /locus_tag="ACHAUN_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8459509.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9718..9996
                     /gene="comGC"
                     /locus_tag="ACHAUN_000785"
     CDS             9718..9996
                     /gene="comGC"
                     /locus_tag="ACHAUN_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8459510.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9971..10441
                     /gene="comGD"
                     /locus_tag="ACHAUN_000786"
     CDS             9971..10441
                     /gene="comGD"
                     /locus_tag="ACHAUN_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8459511.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10413..10760
                     /locus_tag="ACHAUN_000787"
     CDS             10413..10760
                     /locus_tag="ACHAUN_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459512.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10723..11148
                     /gene="comGF"
                     /locus_tag="ACHAUN_000788"
     CDS             10723..11148
                     /gene="comGF"
                     /locus_tag="ACHAUN_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8459513.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11120..11479
                     /gene="comGG"
                     /locus_tag="ACHAUN_000789"
     CDS             11120..11479
                     /gene="comGG"
                     /locus_tag="ACHAUN_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8459514.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11651..12658
                     /locus_tag="ACHAUN_000790"
     CDS             11651..12658
                     /locus_tag="ACHAUN_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8459515.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12683..13867
                     /locus_tag="ACHAUN_000791"
     CDS             12683..13867
                     /locus_tag="ACHAUN_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8459516.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14304..15887)
                     /gene="abc-f"
                     /locus_tag="ACHAUN_000792"
     CDS             complement(14304..15887)
                     /gene="abc-f"
                     /locus_tag="ACHAUN_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8459517.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15992..16120)
                     /locus_tag="ACHAUN_000793"
     CDS             complement(15992..16120)
                     /locus_tag="ACHAUN_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459518.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16465..16608)
                     /locus_tag="ACHAUN_000794"
     CDS             complement(16465..16608)
                     /locus_tag="ACHAUN_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8459519.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16831..17310)
                     /locus_tag="ACHAUN_000795"
     CDS             complement(16831..17310)
                     /locus_tag="ACHAUN_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8459520.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17383..17667)
                     /locus_tag="ACHAUN_000796"
     CDS             complement(17383..17667)
                     /locus_tag="ACHAUN_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459521.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17745..17939)
                     /gene="ssrS"
                     /locus_tag="ACHAUN_000797"
     ncRNA           complement(17745..17939)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHAUN_000797"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18011..19297)
                     /locus_tag="ACHAUN_000798"
     CDS             complement(18011..19297)
                     /locus_tag="ACHAUN_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8459522.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19435..20106
                     /locus_tag="ACHAUN_000799"
     CDS             19435..20106
                     /locus_tag="ACHAUN_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8459523.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20072..20560
                     /locus_tag="ACHAUN_000800"
     CDS             20072..20560
                     /locus_tag="ACHAUN_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8459524.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20573..21520
                     /gene="prmA"
                     /locus_tag="ACHAUN_000801"
     CDS             20573..21520
                     /gene="prmA"
                     /locus_tag="ACHAUN_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8459525.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21521..22285
                     /locus_tag="ACHAUN_000802"
     CDS             21521..22285
                     /locus_tag="ACHAUN_000802"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8459526.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22518..24731
                     /locus_tag="ACHAUN_000803"
     CDS             22518..24731
                     /locus_tag="ACHAUN_000803"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8459527.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24742..25188
                     /gene="dtd"
                     /locus_tag="ACHAUN_000804"
     CDS             24742..25188
                     /gene="dtd"
                     /locus_tag="ACHAUN_000804"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8459528.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25359..26618
                     /locus_tag="ACHAUN_000805"
     CDS             25359..26618
                     /locus_tag="ACHAUN_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8459529.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26707..27654)
                     /locus_tag="ACHAUN_000806"
     CDS             complement(26707..27654)
                     /locus_tag="ACHAUN_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8459530.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28048..29340
                     /gene="hisS"
                     /locus_tag="ACHAUN_000807"
     CDS             28048..29340
                     /gene="hisS"
                     /locus_tag="ACHAUN_000807"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8459531.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29355..31124
                     /gene="aspS"
                     /locus_tag="ACHAUN_000808"
     CDS             29355..31124
                     /gene="aspS"
                     /locus_tag="ACHAUN_000808"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8459532.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31233..32108
                     /locus_tag="ACHAUN_000809"
     CDS             31233..32108
                     /locus_tag="ACHAUN_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8459533.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32374..34257
                     /locus_tag="ACHAUN_000810"
     CDS             32374..34257
                     /locus_tag="ACHAUN_000810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8459534.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34332..34616)
                     /locus_tag="ACHAUN_000811"
     CDS             complement(34332..34616)
                     /locus_tag="ACHAUN_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8459535.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34606..35340)
                     /locus_tag="ACHAUN_000812"
     CDS             complement(34606..35340)
                     /locus_tag="ACHAUN_000812"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8459536.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35469..36092
                     /locus_tag="ACHAUN_000813"
     CDS             35469..36092
                     /locus_tag="ACHAUN_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8459537.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36284..37405
                     /locus_tag="ACHAUN_000814"
     CDS             36284..37405
                     /locus_tag="ACHAUN_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8459538.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37412..40540
                     /locus_tag="ACHAUN_000815"
     CDS             37412..40540
                     /locus_tag="ACHAUN_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8459539.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40559..43753
                     /locus_tag="ACHAUN_000816"
     CDS             40559..43753
                     /locus_tag="ACHAUN_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8459540.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43899..44606)
                     /locus_tag="ACHAUN_000817"
     CDS             complement(43899..44606)
                     /locus_tag="ACHAUN_000817"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8459541.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14410 a         7972 c         9414 g        13112 t
ORIGIN      
        1 acaaaaaaag cgagacataa gtcttcgctt tttttgtttt cttttatttt gatgaatcag
       61 ttgtttcttc agttgaagat tcttttgttg aatcagttga ttttgtgtca gaactctttg
      121 ttgaagctgt agtttcactt gagtctttcg tacttgatga agttgttgtg aatgcactca
      181 acacattttc aaaggcatca tctttgattt ttacatttgc atcttttaat tcttcaccga
      241 ttactttagt tgtgaatgca ttgtcactta gtttagtttc agtagcgata tcttttagtt
      301 gatctttgta tttatccatg tcattgcctt tattttggtt tttcaccatt ttgacaacgt
      361 agtattctgt cgcataagaa gtaggatttg ttgtagtaat aacatcggag atctcaccgt
      421 ctttcaattt gaaagctgct tcttttactt ctgcaggaat agttgttgtc gttgagtcga
      481 atttaacttt accgccatct tcttttgtct ctgtatcagt tgatttgtct ttggctaatt
      541 tagagaaatc atctccatca tccgctgatt ttttcacatc ttttgcttca tcttcacttg
      601 ataatttgat gatttgtgct tctacttctg gatggaaaga tttccaagca gttttcaaat
      661 cgtcatctgt aatatctacg tgagctttca agccagcttc aaaagctaaa ttgttacgaa
      721 tatattcttt ataagtatct ttcgtatatc cagcagcttc taattgtgat tcaaaagtat
      781 cacctaatga tttcgcttgt ttgtcgtatt ctgcatccac ttgcttatcg gttactttat
      841 cgccatattt gttattgaat actttataaa tgatcatccg ttgtactaag gattggttcg
      901 atgattcaag ttttgcttca tcatagaaat cagatacagt gatcgttccg cctttcatcg
      961 ttgcgatatc tttattagta tcgcctgaac aagctgctag agttaaaaca gccaacgcac
     1021 tagtagctgc caaaataatt gatttttttt tcattaaaat ttgcactcct gtaattagtt
     1081 attattttta tatattaact ataccatatc ccatttcctt taaaaacgtt gtcgtggtag
     1141 tttcaaacaa aattcaaaat ttcatcacat tcctaggaaa tacgcaaact tttctcactt
     1201 atatagttaa atctgttgag aaaataatca ttattatatt tgttcaggat actctggcag
     1261 atcagctttt agtttttcta cttgttcatt gatttgtgtg atacgtggtt cggcttggaa
     1321 tttaaaatct tcgatatctt ttttgattgc ttcttggaaa gcaggaatca cagattcagc
     1381 tgtttctttt gtttcgatca atgcgctttt aaaatgattc aggctgttat ttaaatcatt
     1441 tgtcagagtt gttgcttctt ccaattcttc gatcagtttc tttcttgttt cattcccgct
     1501 cctgggcgcc aataacagac ctccgacagt gccagcagca gctccaaata acagtccttt
     1561 gaaaaagttt ttaatcatcg caggacacct gctttacgat tgtttcttga atagctttta
     1621 gttcttccga tccataacta tcttgatgat tgacaaagtg aatagaaaaa tcatcctctt
     1681 tgctgtagcg agggactaaa tggatatgtg aatgaaaaac agattgatag gctagttcac
     1741 ggttgttatt caagatatta agacctttca tttctggaaa agctttttcc aatgcttgag
     1801 caatcttagg cacacgcgaa aaaacctctc ctgcaagttc gggatcatat tcaaaaatat
     1861 ctgctacatg acgtttaggc acaactaatg tgtgtccttt cgttgcttga gagatgtcta
     1921 aaaaagcata gaccttgtcg tcttcatata ttttatagct tgggatttct tgattgatga
     1981 ttttgcaaaa aatacagttt tccatataaa acatcctttc tataaagaat aaatacttca
     2041 gcttaacttt accataagtt aaagctattg taaggaaaaa aacaaaatac cgtcattttc
     2101 ttcgtggtat aataaataaa aataaatggc ggaggaaaaa gaatgagttt aattatcgat
     2161 catgtcacag gggggtacgg acatctccct gtgttaaaag atatcagttt cgatgtacag
     2221 cctggagaga tagtagggtt gattggactg aacggagcag ggaaaagtac gaccatcaag
     2281 cacattatcg gtctgctgga agcttcaaaa ggaacgatag aaatcaatca tctatcctta
     2341 aagaaaaata cggaagaata tcgcaaacaa attgggtata ttccagagac tccttcttta
     2401 tatgaagaat tgacattgag agaacatatc gaagtcactg cgatggccta cgatatacct
     2461 atggatatag caatgaaaag agcagaaaag ttgttggaga cttttcggtt atctaatcgt
     2521 cttgactggt tcccagctaa tttttcaaag ggaatgaaac agaaagtgat gatcttatgc
     2581 gcctttttga tcgagccttc tttatatatc atcgacgaac catttttagg cttggatcca
     2641 ctagctatca atgcattgct tgaattgatg gtagaaatga aaaaagaagg agccggtatt
     2701 ttgatgtcta cgcatatttt agctactgca gaaaaatatt gtgatcgttt tgtggtctta
     2761 cataacggag agatacgagc agacggttct cttgagatgt tgagaaaaga atttcagctg
     2821 ccggattctt ctttggacga aatctatatt gctttaacaa aggagcaggc acatgagtag
     2881 tttttttcaa aaacgtttag caagacatca aaagaaaatg atgaaatata tgcgttatgt
     2941 attgaacgat cattttgttt tagtttgtct tttcttgatt ggcggtgtgg gattatatta
     3001 ttctaattgg ttgaaaaccc tgcagccacc gtttgcaatt ggagggctca taatctctgt
     3061 attttggatg ctctgtttat tcgtgggtaa gatggctact tttgcagagg cagcagatat
     3121 tgttttttta cttcctaaag aaaaagaaat gcaaacctat ttggaacgag cttttcgcta
     3181 ttcttgcatt tttccgttga tctttttgat attggcatgc ggattggcca tgccattagt
     3241 ggtggttagt acggagcaat ccttttcgat gtttttcgtc tatctgatca tattatggag
     3301 tttgaaattc agtcatctgc aaatcaagag agcagaattg tttcgactag atcaacaagt
     3361 tattcgttat tggaaattag gctggttttt ttcttctctg atctttttgt ttctatcatt
     3421 ttattggcta atatgggcag gaatagcagg tgcattagta caggcaagtc tatattactg
     3481 gtttttgtgg aagcaacaag aaaaaggatt ggattgggag aaaatggtgc atgtagaaga
     3541 acataggttg catcagatct atcaatttat caatttattc acagatgttc ctgaaattac
     3601 ggcaaagatc aaaagaagac gctatttgga tcctatattg actcatatta aaagagaatc
     3661 aaaaaacact tatctttact tgtttgcaag aagattggca agaggttctg attttagtgg
     3721 tctttatttc cgattagtaa ttataggtgg gttattgatt gccggagtag tcgactttta
     3781 ttttagtctg attatcggtt tcttattttt atatttgatc ggtttccaac tgttaccttt
     3841 agcgaaccag ttccgctata tggttctcgt tcagctttat ccaatccata agaatcagaa
     3901 gaagcaggcg attcagtatc tactcacatg ggtattaatt atcgcgtcgg ttatttttgg
     3961 tttgattgct gcagtcgtat tagatgggca gcagcgatgg gttccgctag cggcttattt
     4021 gattgttact atgctctttg ttaaactata tgtaccaact cgtttgaaga agatgtcaga
     4081 ctgaaaacaa agctgtgtag ggtaaaaatg agaatgaaaa atagagcgtt gacagtaaga
     4141 atgctgttcg ttctattttt tttgagaaaa gctggttttt tgaatggcaa ttaaatgaaa
     4201 aagaacgtat atgtgcttga cgtgtagtgt tcaggcgact agaataataa gtactgcaaa
     4261 acaaagagta agaggacggg agaatgcatg atggagtttc agttagacca agagtggcgg
     4321 ttacgtccca tcaaaggcga cacaggaaaa acatacatag gcatgcgtga tgaggacaaa
     4381 gtctttatta aacgaaacac gactccgatg ttggctgctc tttcaaggga aggaattaca
     4441 ccaaaacttg tttggacaaa acgaacgggg aatggcgata ctttgacagc acaagagtgg
     4501 ctggaaggtc gcttgctgac tcctaatgag attggtcaaa gaaacgacgt gattgatgtt
     4561 ttgtaccatt tacatcattc aaattctctt aaaaccatgc tgacccgtat cggtggtcgt
     4621 gtcatgacgc cggagaagat gctgcaaaaa tatgaaaaag aattaccgga agaactccag
     4681 aaaaatcaat ttctgcaaat tgtccatcgt tttcttcgtg agcagatccc gtcaatagat
     4741 aaggaaaact tatcagtagt acacggagat gtaaaccatc gtaattggtt agtatgtaaa
     4801 aattatttat atctagtcga ttgggattca atcatgtttg cagaccctgc ggtggatatt
     4861 gcaacgattt taggaaatta tgtgccactg tctagctgga atcagtggct gatcagttat
     4921 ggcatacgcc cgactgacga aatgcttgaa aaatttcatt ggtatgcggc catgaatttg
     4981 ctccaagaaa tcaccagata ttgcaaacgt gaagatcatc gacggatgaa cgaagaaatt
     5041 ttacaattaa aacgaatttt cagtggctga ggtttggccc aaactctgcg gagatttggc
     5101 cagacctttt taattagagg aaaggaatgg agataaataa tgcgtgtccg aaatcgtccg
     5161 ggagcagcgg aaatgttagc ggctcacccg aattttgtaa ttagtgatcc aacattgtgg
     5221 aagggaaaat ggaatgaatt gtttgaaaac gatcatccaa tccacattga gattggtatg
     5281 gggaaaggcc agttcatcac tggaatggca aaagcccatc ctgaaatcaa ttatattgga
     5341 gtagaaatgc aggtcagtgt tgtttcgatt gcactagaca agttgatcga acaaccgctt
     5401 cctaacctga aacttctaca tgtggatgga tcagcgttga cagagtattt cgctgacagc
     5461 gaagtagatc aaatctatct gaatttttcc gatccatggc caaaaaaacg tcatgaaaaa
     5521 cggcgattga catataagac ttttcttgct gtagatgaac agattcttcg tccaaatgga
     5581 gaaatccatt tcaaaacaga taatcaagga ttgtttgagt attctctggc aagtttttct
     5641 caatatggca tgatcctcaa acaggtatgg ttagatttac atcaaagtca atttgaaggc
     5701 aatattatga cggagtatga agaaaaattt tcttcaaaag gtcaacgtat ttatcgagta
     5761 gaagctcaat ttcaagataa gaccaaaaag aaaagtctgt gaaactaact ttctgctttc
     5821 ataaaaacat taatatccat aaataaatcc cagaaaatga acattacaga tgtaatgtat
     5881 tttctgggat ttatttgatt taaaatgtat ttatttcatc ggtttttcca caaggtggaa
     5941 tcaattggaa gcgagaaaat gaaaataatc ctttgtcggt gatcttcaat tcgggtatga
     6001 cagataatgt taagaaagaa agtgtcaaaa aaggatcaaa ggttttagct gctcctagtt
     6061 gatgtgcttt agttattaat tcattcaact gttcatagac gatcgaaaaa ggttcctgtg
     6121 tcatgattcc gcctatagga agagaaacac aagctatttc ctgctgtttg ttgacaacgg
     6181 tcatcccgcc acctttttga atcaaaagat tagcagcata cagcatatcc tcgtcgtttg
     6241 tcccaacgac aactagatta tgcgagtcat gggcaatcgt tgtcgctatc gctccttgtt
     6301 tcagctgaaa tcctttcact atgcctaatc caatgtttcc cgtttgatgg tgacgctcga
     6361 tcaccgcaat ctttaattgg tctttttcga ttgaaggaat gaatcgttcg ccttgtcggt
     6421 ccactttttc taccaaatct ttagtcacta gactattcgg gataatttcg atcacatggg
     6481 cttgtgaaga agaaagaagc agctctaacg aatgtggatt taatggttga gcgtttagtt
     6541 ttggcagaca acacgtatat gaatttgaat gatcagcaaa taaatggtct tggagttgcc
     6601 cagattgaac aactaatgtt cccttataga aaacttcatg aattggcagc ttggtaagat
     6661 cgtctgttaa gaagaaatca gcttgatacc caggggcaat cgcacctagt gttttcaaat
     6721 caaaacattc tgctgcattc aatgttgcca tctgaatggc ttgtaatgga tcgattccaa
     6781 gtgcaatagc ttgacggacg atatgatcga ttgatccttc ttttactaaa tcattgatca
     6841 gtttatcatc ggtgacaaag aagcatcttc ttgaattttc aggtgtgatc gccggtagta
     6901 gcgatgccag atcttttgca acggtccctt ctcgaaccat taaatacata ccaagatcca
     6961 aacgttcttt agcttctttt gcagttgtag cttcatgatc cgtccgaata cctgctgcag
     7021 gataaacatt taactcttcc atgccgatac cagctgcatg cccgtcaatt tttttcttat
     7081 gctggtgcat caattggatc ttttctagga tgtctgtttc attatttgct actgcttgat
     7141 agttcatgac ttcagctaag cctaaaactt cgggtttttg taaaaaaggg gctaattttt
     7201 ttgcatttaa atttgcacca ttggtctcaa aaggggttac aggtacacaa gaaggtagca
     7261 taaaaaagat attcatgagt gtttgtcttg catcttctat cataaactca agacctgtac
     7321 tcccagcaat gtttcctatt tcatggggat ctgtcactac agtagtaact ccatgaagca
     7381 aggatacttt tgaaaattcg cttggtgata aaagtgagct ttcgatatgc acatggccat
     7441 cgatgaatcc tggcactaga tattgcccat tagcattatg tacgcttttt ccttcgtatt
     7501 caccgattcc tacgatcatt ccttcgcaga ttgctacatc tttttccatg atttctttcg
     7561 taaagacgtt gacgatctta gcatgcttga tcactaggtc agcaggcttt tttttacttg
     7621 ctacttcgat atatttcctt aatgttgttt ccaatggagt gcctcccaag ataaattttt
     7681 ataagtataa cataaattcg gtttttgcca atattttttt gtaaattcat tttatttgaa
     7741 acaaaaagat caaaaagacg tatgtttttt gtggagggga gaataatgga aataaaacga
     7801 ttatcggatc aaatgataag tgaaagtttt gccaaacatg cccaagacgt atatatattg
     7861 ccagcttcag aagtatttta catctatttt cgctatggaa aaaaacgaac attctggaag
     7921 aaagtctctt cggcagatgg acagcaactg atctcaagat ttaagtattt gggacagatg
     7981 gatatcggag aaaaaagaaa agctcagtta ggtgcattta cttattcttt agaaaaacag
     8041 gaagttcgtc tgcggttgtc ttctgtagga gattaccttc aacgagagag tttagtgatt
     8101 cggctattat acggtacttt tgaaacaccc catcgttgct ttagttctcg agattttgca
     8161 actattttac agaaaaccgt cagacgtgga ttataccttt tcagcggacc agtcggatca
     8221 ggcaagactt ctctaatgta tcggttggct ttggaagaaa aattgcaagt gattactatc
     8281 gaagatcctg tagagataga agaaccgttt tttctgcagc tacagatcaa tccgaaaata
     8341 gcacagactt atgatacact tttgagactc gcattacggc atcgtccaga cttgctaatc
     8401 attggcgaga taagagatga atcaactgca catgctgcgg taagagcagc actaacaggg
     8461 caccgggttt ttgcaaccgt acatgcacgc aatcttgaag gtacattggt cagaatccat
     8521 gaactgacaa agaaaaaaga agagctatct gaatgtttag ctggtgtcat ttatcaagaa
     8581 cttttggaag atgccaatca ccatccttct gttttatgga gttatcaatt tatggaggaa
     8641 gaatcagtaa aaaaatcatg ggaggaaagt tatgaagaag ctcaggaaaa tatttggcag
     8701 tcagaactat tctaaaggat taacaagaag ccagcaaaac cagtttgcac aaaccatggg
     8761 agaactgctt ttatctggtt ttagtctgca agaatccctt tatttattac tcacgatccg
     8821 ttcgtttcca cctgttattt tggcaaatat ccaacatcag ctaaaggaag ggcaagcgtt
     8881 tagtgaggta ctttcgcaaa taggatatgg agcagagcaa ctggtacaga tcgagttagc
     8941 tgaacgtcat gggaatctgc cgacaacttt gttggaaatt gccaaacagt ttcggcttgt
     9001 tgaaggattt cgcaatgatt tgaaaaagct gcttgcatat ccgatgctat tgctcacctt
     9061 tttgctaggt atcttgttta gtatgcggtt gttcgttttg cctcagctta ttggttcagg
     9121 aatggtcatg caagagcatt ggggtattca gctgatacaa gtctttcatt ggtatgttct
     9181 actattcatc ctagcggtta ctctattggc tttactagta aggtatcgtc tgatgaagtt
     9241 gtctcctgtt gatcagagtg aatggataag cagacaatta ttgattggac cgatatattc
     9301 cttgtatcaa tcggcttatt tgtcactaga gattggaaag ctttttcatg aaggtttcga
     9361 attaaggcaa atcatagtct gcttaaagga aacgaaacaa gaaagtctga ttcagcatct
     9421 tgcctctaag ttgattcaag gattggaggc aggtatgtca ttggctgagc agttccagaa
     9481 ttatcgtttt ctatcagaag aattcagtaa aattgtttta cagggagagg caaagggaag
     9541 tttaggtaaa gaactgattt tttacagcga atggacaaga aaacagcttt tcaaacgtct
     9601 tcatcactgg atgcatttta ttcagccaat catttttttg ggcgtagcgg ctttgatttt
     9661 attagtttat gccgctgttt tattacctgt ttacggaagc atagaggagg ttttgccatg
     9721 aaaaagagaa aaatatctta ttatagcggt tttacattga tcgaaatgct aatcgtctta
     9781 ctgatcatca gtgtattagt gttgttgttc gtccctaatt tgtcgcgtta taggaatcat
     9841 gtagaccaag aaagtcgtga agcgatcatc cagttggtag acacacaaaa agagctctat
     9901 gcgcttcaaa ataatggaag agttcctact gtggaggaat tgttgaatga aggctatatc
     9961 aaacgagaac atgcagaaat ctatcagcgg ccttgaaggt aaggttttag catcaggata
    10021 tacgttgttt gaatcactct tactgatttt attacttaca acgatccttt cttttccagt
    10081 tctcgcattt tctgcatgga agaatgaatt ggcagtctat caatttttcg gccaatttga
    10141 aaaacgaatc tattctacgc aaaaaatcgc tattgtgaat cagatccaaa caggctttta
    10201 ttggaatagt gaagagaatc aaatcatttt tcatgttccg aatccagata aaataaattg
    10261 ggaagtatta gatattcctg atgagattac cttgaaacgc catgcttcta ttacttttgc
    10321 tgctagaacc gggaatgaaa gttcactaaa agcttatcaa ttttattggg aagaaaaaga
    10381 gcggacgatt acgtatcaat tccagatagg gagtggtcgc tatacaaaaa gaattgaata
    10441 aaacaggatt tgttttgttg gagtccctta cagcgcttat gatcgcttct gtctgtgtct
    10501 acttagtgag ctttggccag ctgtttttgc ttcaggcaga aaagaaaagt ggggaggaac
    10561 tgctatttat tcgaatgatt tatgaagata tcaagacgca gcggatgtat cacaaaagac
    10621 cagagcgaag ggaagaaatg atcccatacg atatccaaat tgtcgataat aatggagtaa
    10681 tcgaaaaagt gtttgtcaat atggagaaag aagcgatcag tattgaaaaa acaagtaatt
    10741 ccagcattta cgctgattga atgtctaatt ggactgatga tattgagcag tctattgctc
    10801 acattcaatc ttttgattca gcaaaccatc caagtaagtg aatttttgca gcgtaaagat
    10861 caaaaagagt ggctaatatt tttagcacaa ttagaagaag aactcaagag cagtcacaat
    10921 gtctctgtga aaaatcagca actgcactac acgatcgagg ataaacagta tatcatcgaa
    10981 agatatcagg caatgatacg taaaagaaga acttccggcg gacatcaacc tatgttaaca
    11041 agtatcactg agcttcaatt attggaaaaa gaacacacaa tttcttttta tgtacacttt
    11101 gaaaatggag aagatggata tgccatatgg acaaaaaacg atcagtagaa aatgttataa
    11161 gggctctatt ttattttctg tcttgctttt atttctttta tgtacgtttt tattgatggc
    11221 agcattagaa agctaccgtt tgtcaatgga tcttaatata cgaatcaaaa attattatat
    11281 agcaaaaata atgaagcaga tggctttatc ggaacagtct acgcttgccg aaagttcgga
    11341 aggagttttt tactacacaa ctggatctgt aacatatcag tgggtacaac agtccctatt
    11401 cttagaggtg gaagtctcac cgtttatctt tcggttcaca gaagaagtaa aaaatgatac
    11461 agatactagt acagaatgat ggaatgttcg tgaaaacgcc ctcttttctg ttataacact
    11521 gtttttaata aaaaaatgtt tgccgtttca caaataaaaa acagtgaaaa cgattgaaat
    11581 tttgtcaatt gttcgataga attaaacagg tgaaaaaatt gttataatat atctagaaat
    11641 gaggtgcacc atgttaccag aaaaaatgga acaagggttt aatcaaaacc tggaagctat
    11701 tcagctttta cagaatgcat tgggaacttc ctttttagaa gcctatgtcg aaaatgcaga
    11761 aaatttaata gatgattatc aagtacgtgt ggtggatgga gtgccgacaa aagagacaac
    11821 gcaacggatc acagctcttt atgaagagtt gaagaaactt tctttcgagc cagaagaatg
    11881 gcgcagactg tctcaattac ttttattgaa gggcagccag acagaacatt tgcaggcgaa
    11941 ccatcaactc actcctgata gtatcggttt tttatttgtt tttcttattg aacaactata
    12001 cacaaataaa aaagcaccgg ttaagattct ggatattgcg gcaggaatgg gcaacttgtt
    12061 attaacagtt cttttaaacc tatcgaatgc ggggtaccaa acagaaggaa ttggtgtgga
    12121 tattgatgat acattgttgg ctgtcgcagc ttctacaagt gatttgacac aagcaaatgt
    12181 tcaatacttt catcaagacg ggttgcagga attattaata gatcctgtcg attttgcaat
    12241 cagcgatttg ccaatcggtt attatccgaa tgacgaaaaa gcaaaagagt ttttaacaag
    12301 taccgaagaa ggccatagtt atgcacatca tttattgcta gagcaatcaa tgaaatatgt
    12361 gaagccggat ggttttggtt tgtttttgat gccgagtgga tttttagaaa cggatcaaag
    12421 tgaagaaatc aaaaaatggt ttaaagaaga aggttatctt caaggaatga ttcaattgcc
    12481 agatgaattg ttccgtaata aacaatcgca aaaaagtatt ttgatcttac agaaaaaagg
    12541 accacaagca caacaagtaa aagaagtctt gcttgtgaaa cttgcttcat taaaagaacc
    12601 agagaaggtg acggaatttt tcaacgagtt taaaaactgg aaatcatctg ctctgtaatc
    12661 attcaacgag aaggagagtc atatgtctaa aacaattgca atcaatgctg ggagttcaag
    12721 tttgaaatgg caattatatc aaatgccaga agaaaccgta gttgcgaaag ggatcgtcga
    12781 acgtatcggt ttacaagatt ctatttttac catcaagtac ggcgataatc aaaaattcga
    12841 acaaatcatg gatatcgaaa accacgaagt agcagtaaaa atgctgttag atcagttgat
    12901 tgaactgcaa attttaagct cttatgatga aatcaccgga gtagggcacc gtgttgttca
    12961 cggaggagaa acttatgggg attcagtagt gatcgatgag aaagtaatgg accgcattgc
    13021 tgaattagca gaatttgctc ctttgcataa tcctgcaaac ttaatgggga tcaaagcgtt
    13081 taaaaagatt ttacctgaca ttttgagtgt ggctgtattc gatacatctt tccactcaac
    13141 aatgccaaag caaaattatt tatatagctt accggtgtct tattatgaag atttcaaagt
    13201 aagaaaatat ggcttccatg gcacaagtca tcgttatgtg tcggaacgtg cggcaaaatt
    13261 attgaacaaa ccgatcgaag aactaaaaat catcacttgc cacttaggaa atggtgtatc
    13321 gatcacagca gtcgatggag gtaaatccat ggatacttct atgggattca caccattagc
    13381 tggggtgaca atgggtacac gttcaggtga tatcgatcca gctgttctcc catatttaat
    13441 ggaaaaatta gagattgata ttgatgaaat gatcaatgtt ttgaacaaga aatctggtct
    13501 gctaggccta acaggattgt ctagtgatat gcgtgatctt gaaaagaact atgaaaaaga
    13561 acatattcgt ttagcttatg acattttcgt ggaccgcatc cgaaaataca tcggcggtta
    13621 tgtgacaacg atgaatggtg ttgatgcaat tgtctttact gctggtatcg gcgagaatga
    13681 cggacatgta cgtagtgaaa tcatcaaagg tatgtcttgg tttggttgtg aaatcgatcc
    13741 agcgttgaat gaattgcgtg gtgaagaagt agatatctct acaaaagaat caaaagtaag
    13801 agtattagtg atcccaacag atgaggaact aatgattgcc cgtgatgtgg agcgtctgcg
    13861 cggctaagcc aaagcattcg ttaggataaa agatgaaacg catgcaagag ggaattcttg
    13921 catgtgtttt ttttgcggag aagaccgcaa aatggtaatt taacttcaat ttcccttctt
    13981 ttttacagga tcttcttagg gaattttgct tggaaaagtg gtcaaaaggg aaaagactgg
    14041 cattttattt attgattttc aggaaaatcc caatatttta ttttcattca tctttataag
    14101 ggacattaca tatgtaaaaa gaaaaaacat ttttagatca gcctcgtggg ctgtcgactt
    14161 tattcttcgc tttatgttta gctagaaaac cgatccgaaa cattcattga tcgaataata
    14221 aaaacttgat tttccacctg tactttgcct gaatcgtgaa agcacaggta tgaaaatcaa
    14281 gttttttgtt gcgaaaaaac gtcctagtca atcagttggc cgagcggctg gcggctgatc
    14341 tgctcctgat caataacgag tgcttcttgg aagtgttgct gcaaaaagta gcggtcatgg
    14401 ctgactgcta ggagcgttcc ttgatagtct tgcagcattt cttcaatttc ttctcgagca
    14461 tagctgtcga ggtgattggt tggttcatcc aaaatcagga cgttgatttt ttctaaaaag
    14521 agtttcagca acgccaaacg gattttttca ccaccggata aatctttgat ccgtttaccc
    14581 acatcgcttt gataaaagcc acttttggct aattgccgtc gagcgacttc ttcgtttcca
    14641 gtcaatgtga tggcgtaagc cagcaaacgg ctatcgggat cggcaaactg cagctgctgc
    14701 ggcaaataac cgatttgcaa gctactacct aatttgatgg tgccaccgta aggcttttcc
    14761 tcaccgagta ataatttgag taaggttgat tttcctgcac cattgtcgcc gatgatggct
    14821 aaatgatctc cacgatagac tgcaaaactg ctgtctttga atagtacttg atcagcgtac
    14881 tgctggtgca agttttgaat gatgaaaact tctttgccag attttccacc attagacaag
    14941 ctttgcagcc gatttttcgg gggaggtggc ggtttgacga gtgtcagttt cgccaggcgg
    15001 cgttccagtt ctttggcttt tttgaagaag gactcattgt ctccttcatg tgcccactga
    15061 cgaaaacgac gaatcatgac tttcaaccgc tggacttctt tttgctgcag ttcgtaattt
    15121 tttgtcagct cagccaagcg agcttctttc aagactcgaa aacggctgta gtttccggga
    15181 tactcaatca atgcaccttc ttccagttcc catgtttttg tggtgacttg atcgagaaat
    15241 tgccgatcat gagaaacgac cagaaccgat ttttttgtat tctgtaaata gttttccaac
    15301 cagcgaatac tgttaagatc taaatgattg gtcggttcat cgagtaaaag taaatcggca
    15361 tcggctgcta aaattttggc taaagcgact ttaactcgtt cgcctccgct aagttctgtt
    15421 aaccgtgctt cctgacggtg tggaatccct aagccttgca agatcgctgc aatccggtct
    15481 tctaatacgt aaccgcctag ttcttcaaag cgatgctgca gctcaccgta acgggccaga
    15541 cacttctcta agtttgtatc tggttctgtc atcaatgctt cgtagcgact catttgctgt
    15601 ttgaggtcct gtatctcagc ggcagaatgg agcaagtagt ggttgacggt cgcttcggaa
    15661 actgtaagtt cttgttcgac cgtcccgatc tttaagcctt ttttgcgact gataacaccg
    15721 ctgtcgacac cttctctgcc ggtcaagatt tgtaaaaagg ttgatttgcc agttccgttg
    15781 ataccgacta aggcaatttt ttcaccagct tcaatttgac aggataaatc ctcaaataat
    15841 ggattcccgc cgattgtttt tttaatagaa attgcttgta tgatcatgat ctctctcctt
    15901 gagaccactc aaaaaggcca tggttatcca tggcctgtgt tatttaaact cagattatca
    15961 tgagaaaaat accttgagat ggccattgat tttacgtgaa ttcaactgtt ttggacaaac
    16021 ttgtcccgtc aacagcaact gtaaaaccaa ttgcatggct aaatgcgata tggtatcgtt
    16081 ggtaattttc attacttgtt attttagtca tgcgcatcat agtattttcc ctccttcgtg
    16141 tagttgtctt ttccagtgta cctgaaagtg tcagaaagtt caactagcgc tgacatttac
    16201 ttaatattta tcgatgtgta attctgtcag agagcttttt ttatctgact gagggtaatg
    16261 tctcgtggac cacatcattg tattctgagc taaaccagcg ttcaggcgaa atcatatggt
    16321 gtttgataaa aaactttgtc tacatgactt ccttttgcca tttgcttaat accgttgcat
    16381 taaacagctc ttctctttgg aaatctcacc aacatcgcgt tcaggagtat cgttgtggtt
    16441 gcaatggcaa gcggtagcct ttcatcaatt cttgaatgat ttaccgaatg tagcacttgc
    16501 cttctcgcgg tacccgaacc cgaaataaaa ttttagtttg gcgataaaat cggtatccgc
    16561 catcgatact tcatattttt tcgtatcaga ttcatcggaa acttccacat tgacgaacca
    16621 ataagctttg gcatgcttag gtcgttttaa accacaccag ggcgactaat cgccctggtg
    16681 ttcacttttt tttattttac ttgaactcat tgactgtttt ccctttattt taaacattat
    16741 aaagaccgat gagtgcttgc cgcgtctttt gtttttatca cggttttttg tgcaaaaaaa
    16801 cctgccgata gttggcaggt cgtctacgtg ttaagctttg gtgtggtctt tagctggcaa
    16861 ttggttttct aaatcagttc ggacaacaag cacatcgcaa gctgcgtttc taataacata
    16921 ttcagaaaca gaaccaataa ataaacgttc tacagcattc aatcctgtgg ctcctaacat
    16981 aatcaaatct acttgattat cttctgggat ttctctggca ataatttgtt ttggtgagcc
    17041 atattctacg acagaggtta cattattcaa gccagctttc ttcgcattgc tttcataatc
    17101 tgccaatgtt tgctttgcca tttcagtagc ttgttctgca agcattccat caaaggatga
    17161 aacggtttgg aaagaacgtg tgtcaatgac atgtgctaat agaagttctc cgttatttcg
    17221 gatagctaca ttgaccgcct ttttaaaagc taattcagct tcatcagaac catctacggc
    17281 aaccatgatt tttttgtatt gttgtaacat aagcattcac tcctcaagtc aatcgtattg
    17341 tactctttta ttgtagaaca ctttagggaa aaagagaatt atttactgct ttggttcagc
    17401 aatttttttt gcaaatcgga tactgaaggt ggcagaaaca ataattacga tcagtaaaaa
    17461 gaccaaggca ataaagcgtt gattgaaaaa agctgttaaa atacccatta cgcctaaaac
    17521 agcatatgca gctccgtaaa attgtaggaa tccttgattt ttatcatttt tgttgatcaa
    17581 tacaaaaaag atatcctgtt tattaaataa ataatagcta acgaataata atactaaagt
    17641 ggcgataatc attaaaatcc taatcatgaa caaatcctcc aaaataagca acaaatagaa
    17701 aaagcaattt gccaagagca aattgcttta ttctttaaaa gatggtaatc tccgttaatg
    17761 ccgttgtttg tcctgtagta ttgatcccgc gaattcaagc aggtgggtcc catggtcgta
    17821 gcttcgaact accaaatgat aaggcatgtt accagcagtg acacaagacg ggtcccaaga
    17881 tatcaataaa aatgttcggt caacacacaa agaggacaat tcgaacatca cagatttccc
    17941 acctctatag tagcatatga atttaaaaag agcaatgaga gagctcgcag aaacagaacc
    18001 gttttcttgt ctagtttttt ggaggatgct ttttccactc ttttatacgg taatactgtt
    18061 gacccagagc ctgttcatat ttccctgtat cttttggctg ataatactga gcattcttca
    18121 gtttatctgg taaatattgc tgattgaccc aagcctgatc aaaatgatga ggatactgat
    18181 aacccacgcc acggttgagt gatttcgcac ctttgtaatg gctatctctc aaatgatcag
    18241 gaacatctcc agctttgccc tctcgaatat cagcaagtgc tgcatctaat gccatgtagg
    18301 cagaattgga ttttggcgac aagcaaagat cgacgacgac gtctgctagt gggatacgtg
    18361 cctcaggcaa tcccaatttt tcagcagcta atacagcgtt cactgtacga gccgcagcag
    18421 caggattacc taaacctata tcttcataac cgataaccat cagacgccgg caaatacttg
    18481 ccagatcacc tgcctcaact aaacgggcga gataatgaag tgcagcatcc acatcacttc
    18541 cacgaatcga tttttgaaaa gccgaaataa cgtcataatg agcatctcca tttttgtcat
    18601 gggtaagagc ttttctttgg acacattctt caatgatcga cagtgtaaga tgaatccttc
    18661 cttctttatc tgaaggagtc gatagtgtag ccagttccag cccattcaat gcactccgca
    18721 aatcgccgtt cgttgcacgc gaaaggtgaa gaagagcatc ttcgtctaac tggattgctt
    18781 gttgtccaag gccgcgttct ttatccctta aagcatgttc gactgccaac tggatgtctt
    18841 gttctgttaa tggtttgact tcaaagatct gtgtacggct gcggatcgct ggattgatgg
    18901 taatataagg attctcagta gtcgctccga tcaagatgat tcggccactt tctaaatgag
    18961 gaagcagaaa atcctgtttt gttttatcta atcggtgtac ttcatccaat aagaggatca
    19021 ctgtaccact catttttgct tcttccgcta caacttgtag atcctttttt gaatctgtag
    19081 cggcgtttag catgcgaaat gcataattag tagagccggc gatcgcactt gcgatactcg
    19141 tttttccagt accaggaggt ccatatagaa tcatggaaga gagcatgcga gcatcgacca
    19201 tgcggcgtat gatttttcct tccccgacta gatgctgctg accaacgact tcatcgatcg
    19261 ttcgaggtct catgcgataa gccaatggct gttgcatagg attttcctcc ttcttgtttt
    19321 tacgtccctt aattatatca tatttagcaa gatgcctaga aagaacagcc ttctgttgtt
    19381 ttgttttcat agcttatcca actaatattg tgtatgataa agtaggtgag aaaaatgacg
    19441 gataaacaaa agatagatga atttttttgg aataaaacga cgccagaagt ggcacgggat
    19501 ttattgggga tgtatttgga gcatgacaca ccagaaggaa gattagctgg atatattgta
    19561 gacgcggaag catacctagg tccagaagac gaggccgccc atagctatgg tttaagaaga
    19621 acaccgcgtg tgcgggcaat gtatgaaaaa ccaggtacga tctaccttta cacgatgcat
    19681 actcatcgga ttttgaacat cattactcag ccagaaggga ttcctcaagg agtaatgata
    19741 cgtgcaatcg aacccgccgc aatgattgat caaatgagta aaaatcgtgg cggaaaaact
    19801 ggtccagata tcagcaacgg tccgggaaaa ttagtggaag cattagcaat tcctcaagaa
    19861 ctttatggtc agtctatagc agatagttcg ttacgcttag tttttgagaa aaaaaagaca
    19921 ccaaaaaaga tcaatgcttt gcctagaatc ggtatcccaa acaaaggtgt atggacagaa
    19981 aagccgttgc gttttgttgt gtcgggaaat ccttatatct ctttacaaag aaaaaaccaa
    20041 atcgagaaag attggggttg gaggaaagaa aatgaaaaag aaggatctat taacatattt
    20101 agatgaaata attgaaaaac aagtgacaga ctatgatgta gctttagatt gggatacaaa
    20161 aaatcatacg atcgagatcg ttgttcgctt gtttgcagaa aataaagcac aatttgagat
    20221 tgatgatgca gaagggatcg tttcgcaaga accaattatc gaatttgaag acggcgtact
    20281 tctttttaat cctcagaaat ctgtttttga tgaacgcaat tatctagcag taatccctta
    20341 tgaaggaaaa aaagggttgg caaaatcagt agctgattct ttagtggaat atctaaatga
    20401 agtattagct caagggcaaa gcgatttgct tgattttttg gatgaagacg atgacgaagc
    20461 tgtattcgag cttcactggg ataatcagca actagcagaa ttagtagaag taaaacagca
    20521 aaatgaagca gatacttatt taccgtaccc aagttattaa ggagtgacag atatgaaatg
    20581 gaatgaagta aaagtagaaa ctgaaagtga agcagtcgaa gcagttgcta atattttgat
    20641 ggaggcaggg gcgagtggtg tcgcgattga agattcttta gatgttgaac attttcagtc
    20701 ggatcaatat ggtgagattt tagataaaaa tgattttaca tcgctgaaag aaggcgcact
    20761 agtcatggct tattttccgg aaacgacttt cttaccggaa atcttgcctt ttatcaaaga
    20821 tcgtatcaca caattacctg agtttggtct agctattgga aaaaataaag taacagttag
    20881 tgaagtagag gaaagcaact gggcaacagc ctggaagaaa tactatcatc cggtgcgtat
    20941 tacgcgaatg ctgacggttg cgccaagctg ggaaaactac cagacagaag atcctctaga
    21001 aaaaattatc acactagatc ctggtatggc atttggcaca ggcactcatc ccacgacttc
    21061 cttgacattg acagcactag aaacagtctt gcgtggtggt gaaacattgc tagatgtggg
    21121 aactgggtcg ggcgtgctaa gtattgcagc aaaatatctt ggtgcaaaag atgtctatgc
    21181 ctttgattta gacgaagtag ccgttcgttc ggcgaaagaa aatatggata tgaatgaaat
    21241 agccaaagat gtccatgtat cagctaacga tcttttgaca ggaatcgaga ttgaaagtga
    21301 tgtaatcgtt gcgaacattt tagcagatat catcttattg atgatcccag atgcttggcg
    21361 tttactgaag caaacaggca cgctgatcgt ttcaggaatc attgaagaga agaagcaaat
    21421 ggttctggat gccatgttag aacaagggtt tatcgtggat caaattttcc agcaaaaaga
    21481 ctggtatgca attattttga aaaaaccaga ggtggaatag atgcagcggt attttttaaa
    21541 agaatcatat gaatcaaaag actattttgt tttagaaggg gaaaactatc accacattgt
    21601 acgggttatg aggatggaac ccgaggatga agtttttttg gcattcaatg atcaaatagc
    21661 cattgttgca gaaatcacgg agattactga agaggctgtt tatctgaaag aaattaaaaa
    21721 agaagaaaat caaaaggaac tgccaagttc gattaccata gcgagtggct atcctaaagg
    21781 ggaaaagctg gattggatcg ttcagaaatc cactgaatta ggagcgcaag cttttcttgg
    21841 tttcccggca tcagcctctg tcgttaaatg ggatgcaaaa aaacgtatga aacgacaaca
    21901 acggcttgaa aagattgctc aagaggcagc ggaacaatcc catcggcaga tcgtgccatc
    21961 agtagagtta ttggaaacga aacaagattt atttgagcgg ctagatgatt atgacgtggt
    22021 cttggttgct tatgaggaat cagcaaagca aggcgaaaaa tcaaatgcag ccaaaattct
    22081 tagtgaaaca aaaccagata gtcgaatctt agctgtcttt ggaccagaag gtggattatc
    22141 ccgcgatgaa gtcgattatt ttgtagaaca caaagggata ctttgtggat taggaccaag
    22201 aatccttcgt acagaaacag cacctcttta tttattaagt gctgctagct atcaatggga
    22261 attaatgaat ccttctttca gctaacgttc ttcattaata aaagagtatc gacagaaaga
    22321 attgaaaaaa tagtcaacaa tttgtataat agaaacaatt atttatacaa tggatacgaa
    22381 aaatcgtttg aaaaagcttt taaaaagcga acatttccgt aaaaaaacgt ataactgcac
    22441 ggattttggg acaattatca aacagatatt tgtcccgatt cccgttttac cgaaaaaaga
    22501 aagggggatc gattcctgtg acaaaagaag aaattttaac gggacccggt gtcataaagc
    22561 tcgttagtca atacatgggt cccgaacatg tagcatttgt tgagaaagca tgcgaatacg
    22621 cgactgctgc acatgacgga cagtttagaa aatcaggcga accctacatt attcatccta
    22681 tccaagtcgc aggtatatta gcagatttaa aaatggatcc ccatacagtg gctacaggct
    22741 tcttacatga tgttgttgaa gatacagaaa tcactttaga agatctgaga gaagaatttg
    22801 gcgatgacgt tgctatgtta gtggacggcg taaccaaatt agggaaaata aaatataaat
    22861 cccacgaaga gcagctagca gaaaaccacc gaaagatgct gctagcaatg gctcaagatt
    22921 tacgagttat catggtcaaa ttagctgata gactacataa catgcgtacg ttgaagcacc
    22981 tgcgagaaga taagcaaaga aggatcgctc aggaaacttt agaaatctat gcgcctcttg
    23041 cacatcgtct agggatcagc cggatcaaat gggaattaga agatacagca cttcgttatc
    23101 taaatccaaa acagtattac cgtatcgtcc acttgatgca gacgaagaga gaagaacgtg
    23161 aaaaatacgt aagtggtact gttgaagata ttcgaatagc gacagaagag ctggggattt
    23221 ttgcagaaat ctatggacgg ccaaaacaca tttattcaat ctatcgtaaa atgaaagatc
    23281 agaaaaagca gttcaacgaa atttatgact tgctagctat tcgagtgatc gtagattcaa
    23341 tcaaggattg ttatgctgta ttaggagcaa tccacacaaa atggaaacca atgcccggca
    23401 gatttaagga ttatattgcc atgccaaaag ctaatatgta ccaatctttg catactaccg
    23461 tgatcggtcc ggcaggaaat ccggtagaaa ttcaaatccg aacacaggaa atgcatgaaa
    23521 tcgctgaatt cggggttgct gcacactggg cctataaaga aggaaaaaat gaaaaagtag
    23581 aaccagatgg tatgacgaaa caattaagct ggttccatga gatactcgaa cttcaagacg
    23641 aaagctatga tgcttctgaa tttatggaag gcgtaaaagg agatatcttt agtgataaag
    23701 tctacgtctt cacaccaaaa ggagacgtta ctgagttacc aaaaggatcc ggaccattgg
    23761 actttgcata cagtatccat acagatatcg gtaacaaaac cactggtgca aaagtaaatg
    23821 gcaaaatggt gcagcttgat tacaaattga aaaacggaga tatcattgag atcatgactt
    23881 ctccaaattc atttggccca agtcgcgact ggttgaaatt agttgctact agcaaagcaa
    23941 gaaataagat caaacgtttc ttcaaagccc aagatcgaga agaaaatgtg atcaaaggcc
    24001 acgaatccgt ggtcaaatgt attacagatc taggatttac gcctaaagat attttgacga
    24061 agaacaaact gcaagaagca ctcgatcgtt ttaattatca aacagaagat gatctctatg
    24121 cagctgtagg gtatggagaa gttagcccct tgacgatggc caatcgtctg actgaaaaag
    24181 aacgtaaaga acaaaaaatc gagcagcaaa agcaagaagc agaagaaatc atgaatcagc
    24241 cgaaaaaaga acctgacaaa atgaaagtac gtcatgaagg tggcgttgtc attcaagggg
    24301 tagaaaactt actaattcgt atcagtcgct gctgtaatcc gattcctggt gatgatatcg
    24361 ttggttatat cactaaaggc agagggatat ccattcatcg tcgagattgt ccgaatgttc
    24421 agcctgacaa accaaatgta gcagaacgtt tgattgaagt cgaatgggaa gatacatcga
    24481 atacacgaaa agagtatgat gcagatttgg aaatttacgg ctataatcgt tcaggcttat
    24541 tgaatgatgt acttcaaaca gtcaatgcgc taacgaaaaa tctcaacagc gttgaagcac
    24601 ggacgaataa agataaaatg gcgacgatcc atttgacggt tggtatccag aatttatccc
    24661 acctaaagag tatcgtggat aaaatcaaag cagtacctga tgtctacagt gtacgccgga
    24721 cgaatggata ggaggacaga tatgagagca gttgttcaac gggtaagtaa agctgctgtg
    24781 tcagtcgatc agcaagaaat tggaaaaatt ggctacggcc tacttgtttt attaggcatt
    24841 catgaaaccg atacgcaaaa agatgtggac tatctggtaa aaaaaattgc acaaatgcgt
    24901 atttttgaag atgaacaagg aaaaatgaat attagtgtag aagatgtggg aggacaaatt
    24961 ttaagtatct ctcaattcac actgtttgcg gatacgaaaa aaggaaatcg accaagcttt
    25021 gtggaagccg cacgaccaga aacagctatt cctttatacg aatcattcaa tgaaggaatc
    25081 agaagtcgcg gcatcacagt cgctaccgga caattcggag cagatatgtc ggtttctctc
    25141 gtcaatgatg gacctgtaac gatcattatt gattctaaaa acaaataaag taatctggtt
    25201 ttcactagta aaaactatga aacacgtcgc ttaagttaga ctgataaact ctaacttaag
    25261 tggctttttt gtctgcaaaa agcgcttctt ttgtcagcag tcggaaaaaa agttaagctt
    25321 aaattataca agttattttt tataggaggt tgctgaagat gaaagaagta ccagtagtag
    25381 atatcaaaat cacagattta aaaaaattgt atcaaaaagg atacaacatg ttggaggagt
    25441 tacgccatga agcggacgct cccgtcgtca aagcaaagat tttcaataaa gaagctatca
    25501 cgatctacgg atcatcagca gcaaaagtat tttatgatcc aaggaatttc aaacgaaaag
    25561 gtgctatgcc aaaattagta ttgaagacac tttttggtca aggtggagta cagactttgg
    25621 atggagctgc tcatcaccat cggaaaaata tttttatgga tctaatgaca ccggaaagaa
    25681 tggaagatta tcatcgtatc ttagataaga atctgacaca agcacttgaa gcacagcatg
    25741 gtcaatttga actatttgat ttaagtaaga tggttttctt tacatctatt tgcgagtggg
    25801 caggtatcaa tttatcagcg atttctaaag atgaggtcga aaaattagca gaatatcaaa
    25861 tctctatgat cagcggaacg tttacttctc cgattgatca cataaaaggt gtcgaaaatc
    25921 gcaaaaaatc agaaaaatgg gcgcaaggct tgattgaaga agctcgacaa aatcctgtgg
    25981 ctggaaaaga aaatgtggca ttatatgctt ttgccaatgc cacagattta gatggtcagc
    26041 ttttacctct ggaagtggca gctgttgaat tattgaacat catccgtcca acggttgctc
    26101 taactgtgtg ggcggcattg atgggacatg cacttttcag tagaccagat ttgtatcagc
    26161 aattaaaaaa tgacttttcg acgttacaag atccgtttat tcaagaaatg agacgctatt
    26221 atcctttctt cccaatgcta cctgcgattt cgctgaaaga agtagaagta gatggttatc
    26281 gtataccaga aggcagttgg gttattttag atttatatgg aacagaccat gatgaacgaa
    26341 cagttgaagc gccagattcc tttatgatca aacggtatgt agggaaagca aaagatattt
    26401 cctataaaga agaatatgag atgattgcac aaggcggggg aaattttcga caaatgcatc
    26461 gctgtgcagg agaatggatc actcttcata gtctacgtgt gttttccgat cagctagtaa
    26521 ataagtttga gttctctgta cctgaacaag attggacgat tccattcaat cagtttccga
    26581 cttatccgaa tagccgagcg ctactttaca agaattagat tagcataaac ttatcgaaaa
    26641 aaaggaagat caaacagtgc tgatcttcct tttttcatat tactataaat tgataaggtc
    26701 aattgatcaa aatccacgaa atgcttcatc aatggtttga taatcttcat tggataaatg
    26761 caaatctaaa gcttggacat tgtcagatac ttgctctgga cgcttagctc caggtataac
    26821 tacagacact cgtggattct tcatgtacca agctaaaaca agttgcgcag gagtggcttg
    26881 gtatctttta gcaatggggc gaagtacatc gacagcggtt aaaatttctc tgaaacgttc
    26941 tccttggaaa tctggatttc tttttcgagg atctccttca ccaaattgtt tttcctcacc
    27001 aagttcatat tttcctgtca gcaaaccgga agcaagagga aaatagggaa caaaactgat
    27061 tttgtttttt tcaagataag ggaataattc tttttcagcc tgccgatgga tcaaactgta
    27121 tttatcttct actacatcga catatccatc tgcattcgct tctttcaatt gttctaacgt
    27181 gaagtttgac acaccgactg ctcggatttt tcctgcttct tttaattcgt gcaatgtagc
    27241 aactgattca ttttttggtg tgctttcatc cggaaagtgg atataaaaaa tgtcgatgta
    27301 atcagtttgc agacgtttta gtgcatcttc caccgattgt ttgagaaact caggagaatt
    27361 atcaaatgtt cgccctttat ttggtacatg agcagctttt gtagcgatga cgacacgtga
    27421 gcgatcatat tctttttcct tcagtacctc gccaatcagt tcttcggaac ggccgtttcc
    27481 gtaagcataa gcggtatcga tcaaattgat cccgctattc aaagctgtac gaactacttg
    27541 ttttcctgtc tcatcttcca aaccgtcaaa aagattatgt cctccaacgg catttgctcc
    27601 taatccaagc tgttctgcgt agacttgtga atgtccgatt cttatttctg ccatagctga
    27661 ttcctccttt ttgtctagtc taaaacaaaa taccaaaaag ctgaactaaa acgtttttgt
    27721 gaggggaaat agtcagatat ctcttgactt tgagactggt ttttagtagg ttataagtaa
    27781 tatcgaatgt caatgaaaga gcgagtaagt ttttgaaact ttacaagaga ggatcatcga
    27841 ggctgaaaat gatctaaagt ttcattagcc taaagacact ctggagatgg acgagtgaaa
    27901 agccgtcccg cgtacgagcg ttattcgtaa gaggaaagag aaatctttca aaattaggtg
    27961 gtaccgtgta tgaatttcat tcacccttgt tcatgatgaa caagggtgat ttttttattt
    28021 acatagctaa gaaagggaga gaaaactatg agtttccaaa gaccaaaagg aacaaatgat
    28081 attttgccag gcgaatcaga aaaatggcag tttgtcgaag aaacagcacg tttattattc
    28141 agagattatc agtataatga aatgcgtaca ccgatttttg aacactatga agtgatatcc
    28201 cgaagtgtag gggatacaac ggatatcgtt tcaaaagaga tgtatgattt ttatgacaaa
    28261 ggggaacgcc atgtgacgct tcgaccagaa ggtacagcac caatcgttcg ttcttttgtt
    28321 gagcataaat tgttcggccc agagtatgcg aaaccgttca aaacttttta tatgggacca
    28381 atgtttcgct acgaacgccc tcaaaaagga cggttacgtc aattccatca aatcggagta
    28441 gaagcatttg gatcagaaaa ccctgcaact gatgtggaga gcatggtgat ggcacttgat
    28501 ttcttcaaac agttggggat cacgcaaatc cggctagtca tcaattcatt aggtgataaa
    28561 gaaacgcgtc aagcctatcg tcaagcattg atcgactatc ttttaccttt tgaatcagag
    28621 ctgagtgaag actctaaacg ccgccttcat gaaaatccat tacgcgtatt ggacagtaaa
    28681 gacaaacgag atcaaaaatt tgtggcagaa gctccatcta tcttagatta tctaagcgag
    28741 cctgctcaaa aacattttga taccgttgtt tccatgctgg atgcattggc tgttccttat
    28801 gaaatcgaca gtaatatggt tcgcggacta gattattaca cacatacgat ttttgaaatc
    28861 atgagtgacg caccaaaaat gggggcacaa gcaacgattt gcgcaggcgg acgttatgac
    28921 aatctagtgg aagaactagg tggtccttca acacctggct tcggatttgc aatgggtatc
    28981 gagcgattac tcttgactat ggaggcagag gaagtagtta tccctgcatt taatgagttg
    29041 gatgcttacg tggtcgcact cggagacgaa acaaatatcg aagcattgaa agtggtccaa
    29101 gcgattcgaa actttggttt ttcagctgac cgcgacttca tgaaccgtaa agcaaaagcg
    29161 caatttaaga cagcagataa agcgaatgcc aaattagtat tggtgatcgg tgaagatgag
    29221 cttgctagtc aaacgattaa tgtcaaagca atggcaagcc gaaaagaaaa aagttttcca
    29281 ttagaagaga tatatgaaca ttttgatgaa gtctatgatg aaatgacttt attcgagtag
    29341 gaggaaaaaa ataaatggca caaagaacga tctattgcgg attagtttcc gaacaacaat
    29401 tagaacaaac agttacatta aaaggatggg tacaaaaaag acgagacctt gggggagtaa
    29461 tctttatcga cctacgtgat cgtgaaggaa tcgttcaaat cgtctttaat cctgaaatca
    29521 acaaagaagc atgggaaatc gcggataaat gtcgcagtga atacgtgatc gaagtgactg
    29581 gggaagttag aagacgcgga gaaaatgcag taaatcctaa aatggccact ggtgaattcg
    29641 aagtgattgc aagtgaaatc gtgattttga atcaagcaaa aaccccgcca tttcaaattg
    29701 aagatgatca agcaatcagt gacgaaatac gtttgaaata tcgttatttg gatctgcgtc
    29761 gtccaaaaat gactaaaaac atcaaattaa gaaatgcgac aacaaaagcg attcgtcatt
    29821 atttagatga taatgacttt ctcgatatcg aaacacctta cctagggaaa tctacaccag
    29881 aaggcgcaag agactaccta gttccttcac gtgtccatgc tggacatttc tatgcgttgc
    29941 cgcaatctcc tcaattgttt aaacaattgc tgatgaatgc tggattcgac cgttattatc
    30001 aaatcgttcg ttgtttccgc gatgaagatt tacgtggaga ccgtcagcct gaatttacgc
    30061 aagtagatat tgaaacaacg ttcttaacag cagaagaaat ccaaacttac acagagggat
    30121 tgattgcgaa ggtaatgaaa gaagttcgcg gtatcgaagt cacattgcca tttcctcgta
    30181 tgacctatga tgaagcaatg gctcgctacg gcagcgacaa accggataca cgttttgata
    30241 tggaattgat cgatctaagc gatacagtca aagaagtaga attcaaagtc ttccaaatgg
    30301 ctcttgaaaa cggcggtgtc gtgaaagcat tgaatgctaa aggcgctgca gatcgctact
    30361 cacgaaaaga catggatcag ttaggtcaat atgtaggaca attcggagct aaggggcttg
    30421 catggctgaa agtagaagaa gacggattga aaggtccaat tgctaaattc atgggagaag
    30481 caacagaagc aatcattaaa gcaacagatg caaaaccagg tgacttgctg atgtttggcg
    30541 cagataaaag tgaaatcgtc gctgctgcat taggtgctat ccggacacgt ttaggaaaag
    30601 aattaggact aattgatgaa tcgaaattca atttcttatg ggtaactgat tggccacaat
    30661 ttgaattttc agaagaagaa ggtcgatatg tctctgctca ccatccattc acgatgccaa
    30721 aagaagaaga tatcccgtta ttggctgaag accctgcaaa agtctatgca gaagcttatg
    30781 atatcgtctt gaacggttat gaattaggcg gaggttctat ccgtattcat actcgtgagc
    30841 ttcaagaaaa agtactagaa actctaggct ttacaaaaga acaaatggaa gaacaatttg
    30901 gtttcttgtt aacagcttta gattacggat tccctccaca tggcggtatt gcattaggat
    30961 tggaccgttt tgcaatgtta ttagccggag aagataatat tcgtgaagtc attgcctttc
    31021 ctaaaaacgg aaaagcagca gatgtgatga gtgatgcacc aagtatggtt tcgcctttac
    31081 aattgttcga attgaatatt gatgtaacga atatagaaga atagccgata aaaaggtaaa
    31141 aaggatttgt cgaatacgct tcttttgaca accttgattt aattgaaaaa cactttataa
    31201 tgaagccata acatataaag cggggctttt ccatgaaaaa aacattcgaa aattgggctt
    31261 atcatgatta cacgaccaag ctttcggtat ctatcgttta tgcgttgatc gcatcagtcg
    31321 cattgaattt cttttatcag ccaggacata tttattcaag tggtattact ggtctagctc
    31381 agattttgac gactttgtca aaatcactga taggattcga agtaccggta tcaacaactt
    31441 tgtatttact gaatatacct ttgtttgtat tggcgtggtt aaaaatcggg aagaagttta
    31501 caattttcac ttttatcaca gtcactttga cctccctttg tatccatatt ttaccggaaa
    31561 cagtcctatc taccgatcca atcatctgtg cgttgtttgg gggggctgtg atgggactag
    31621 gtattgggct agcgttgaga aacggcctat catcgggcgg tctagatatc gtgagtatca
    31681 cgattcgtaa aaaaacagga aagtcgattg gttctatttc gatctacttc aatgcattga
    31741 tcgtttttgt tgccggaatt ctatttggct ggcaatatat gttttacagt acgttgtcta
    31801 ttttcgtcag tggaaaagta actgatgtcg tttatactaa acaaaagaag atgcaggtaa
    31861 tgatcgtaac gaaacatcca gaaaaagtag taaatgaaat ccaaaactgt atgcgtagag
    31921 ggatcacgat catcaatgaa gcagaaggtg cttataagca tgataagcag acagtattga
    31981 tgacgatcgt tacacgatac gaactgcctc tacttagaga agcaatgaaa gcttctgatc
    32041 cacaagcttt tgtaagtatt gcagaaaacg tccagatact tggtcgtttc tatgaggatg
    32101 gcatttagat cgagtttgac aaaaattcat gaaatactgc tgtagacaga cgttcgtttg
    32161 tctgcagttt ttttctacca tagtagaatc agcaaagaaa aactgctttt ttatttgaaa
    32221 ttttcttaaa gttgaaaaaa agcatgttta tttttttaaa tttatgggat gatagaaaaa
    32281 aatagtagag aaaggacacg attctacttg ctattttcac ttaatacccg tatattacga
    32341 atgaaaaaag tttatgaaaa gttggaatat tgaatggaaa aacaaaaaag gttaaaaaac
    32401 agccgctaca tcactgggtt tgatgggctc cgttcattag ctgtggtggg tgttatcttc
    32461 tatcaccttc tgccaactag tatgaaaggc ggctatttgg gcgttccgat ttttttcgtc
    32521 gtatctggtt atttgatcac ggatctgctg cggcaggaat gggaacaaaa tggaaaaatc
    32581 aatatttggc aattttatgt cagaagaatg aagagactct atccggcact tgtttttatg
    32641 cttgtgacag cttcggctta tatcactttg tttcagcgag ggctgttaaa caatcttcga
    32701 ggagttgtgg tcagcagctt actgtatgtc aacaactggt ggcaaatcaa taacggttta
    32761 tcttatttcg atcgatttgc taatgagtca ccgtttacgc atatctggtc tttagcagta
    32821 gaaggtcaaa actatttggt ttggccgatc ttgtttgtat tgttgatggt atttgttcgt
    32881 aaaagaaaat ggatctttta tactgtgtta ggtacatcct tcctctccgc catcttgatg
    32941 atgatcttgt atacaccagg aggcgatcca acccgtgttt attacggtac cgacactcgt
    33001 ctgttttcta tatggttggg aagtgcgctt gcgtttgttt ggccaagtac gcgtttaaag
    33061 aaaaacatcc cgaaacaagc gaaaaatatt ctgaatatcg ctggaggaat ctcgcttttg
    33121 gcactgattc tttgcttctt ctttttagat gatcattaca atttcatcta ttacggagga
    33181 atgctcctag tgagtctgtt atgcgtagtg ctagttgcag tgacagcaca ccccggagca
    33241 agtttgaatc gatggctgac aaatccagta tttacttgga ttggcaaacg aagctatggc
    33301 atttatctgt atcagtttcc agttatgatt ttttatgaag caaaggtgac tgatattgcc
    33361 gatcatgttt tgttgcatac tttagtggaa atcacattga ttctaatcat cagtgaactt
    33421 tcttatcgat atgtggagcg tccgctagca cgttttgatt atggtcgtac attccaagtg
    33481 atgaaaggat ggttcagcaa accgatcatc agtaagagca aaccatgggt tgttccaggc
    33541 acattacttg tagcaattgc actcgttggt ttcgttacgg caccgaaaaa cgctgtaaca
    33601 gccgatcaaa aacaactgca ggcaaaaatc gctgaaagta aaaaattagc agaagaaacc
    33661 agaaacaatt cagaaaacac cactagttcg gttgaccaag cgatccttga taagtacgag
    33721 ttgtccaagg cacaagggaa taaggcacaa aaattagaac taacggcatt tggtgattca
    33781 gttatgttgg atgctgcaac ggatctacaa gaactttatc caaaagtagt ggtagatgga
    33841 gatgtcggac gacaattata tgccagtgta ccgtacattg aaaaattaaa ggaacaagat
    33901 ttgttgaaag acactgttct gattggacta ggaacgaatg gctcattctc ggaagctcag
    33961 tttgatagtg tgatggatgc aatcggtgat cgaaaagtat actgggtcaa tgtccgagta
    34021 ccgacgaagc gctggcaaaa tgacgtgaat gcgatgcttg aacagatgac gaaaaaatac
    34081 gacaatatga cgctgattga ttggtacagc tacagtaatg accatagtga ctggttttat
    34141 gatgatcaag tccatccaaa tcctgaaggg atgacacatt acattcactt agtcagcgaa
    34201 gccttgctag gaggaagttc aagtacgaac agcagtgaga caaacgtgac agaatgatag
    34261 ctacaaaaaa cttcaggcaa aaacatttgt ttttgcctga agttttttta tatttaccgt
    34321 gtttttaaca ttcatataga ttcaggaagc aaaacggttg agtgtgtctg taaataacgt
    34381 tctttttgtt ttcgggtgag ttttttgaat gcagcttctg cttttgttgc ttcgcttctt
    34441 gaagaaaata cttctgcatg gatcatctta acaggtcttc ttttttgaag acgagtatat
    34501 ttagcaccag taccattgtt atgttcctct aatcgtctag aaagatcggt tgtatatcca
    34561 ccataaaatg tctgatcttt acagaataat acgtaaaaat aatgttcatt tgccatagag
    34621 catctcacga atctctgcag tgtattctcc atccatattg taagtgatca atggtggagc
    34681 gactttgaat ccgtctaatt tgccttcttt gattccttca actaacaaaa tatttgcctc
    34741 ttttccaggt tttgggtaaa caaattggat ccttttaggt gcaattcgat agtcttccat
    34801 gacctgaatg atttctaaga accgatcagg acgatggacc atagtaaagc gtccatttgt
    34861 ttttagtagc ttacttgaaa tatcgatcac ttcttctaac gtcgtatgaa tctcatgacg
    34921 ggcgattgcc aggtgtttat tagggttcgt tttatttgtt ggcagccctt tgaaataagg
    34981 tggattacag acgactaagt cacaagaatt atgtcgaacg acagataagc tgtctttcag
    35041 atcgatggtg tggacagtca tttgttcatt taaatggtta agcgcgatac tacgtcttgc
    35101 catgtctgct agacgaggtt gaagttcgat agcgtcgata gccgcctggg ttcgtttact
    35161 taaaaaaagt ccaactgcgc catttcctgc acaaaggtca acaatcttcc cttttttagg
    35221 tacacgggaa aagtgagcaa gcagcacagc atctaaagaa aaagaaaaca ctttggaact
    35281 ttggataatc ttgatatctt ctgcgtataa ttggtcgatt cgttcgtctt cgtgaagcat
    35341 ggattccctc caaaatgaaa aaaatagtcg tctgtattat actattttta ttaaaaagaa
    35401 ccaaggtggc ttgtcaaacc tctatttttt atgcatacta tgaccataag aagggaaagg
    35461 aagagactat gttttatcga tttatgcgcg gggtagttcg agtactatta gccttagtta
    35521 atggaaatgc ccgctatgag aataaagaag ttttaccgca agatgagaat tacatactag
    35581 ttgctcccca tcgtacttgg tgggacccat tatatctggc ggtagctgca gcacccaagg
    35641 aattcagctt tatggcaaaa gaagaactgt tcaaaaatcc agttctgcga tttatattaa
    35701 ctcgatccaa tgcttttcca gttaaaagag aaaatccagg accaagcgtc attaaaacac
    35761 ctgtaaagat tttgaaatcg acaaatttga gtttgatcat gtttcccagt gggacaagac
    35821 atgctacaga acttaaaggc ggaatggcat tgatttccag aatgtctaaa gcgaaaatcg
    35881 tgccagctgt atatcaaggt ccgttgacac ttggggatct gttcaagcgt cagagagtga
    35941 cagtgcgttt cggcgaacca atcgacttat cagatatcaa gaaaacggat aaagaaggta
    36001 tggcggagat cgaacgacga acacaagcag cttttgacaa attggacaaa gaagtgaacc
    36061 ctgatttcaa atatgaagtc aaaaaaaaat aaaatgataa gtttatgcgt gggcggtctt
    36121 ttgaatctac tgtcctgttc agttcagact ttttcaatga aaacgaggga gagacgtcaa
    36181 gtctgccctc tttttgcgtt ccaatatgta aaatagaaga caggtgtcat gtaatggatc
    36241 aaagtgttat aataattaga aggacatttt ttgaggtgat tgaatgcgtt ttttacatac
    36301 agccgattgg catatcggaa aaaaactaca aggttatgac ttgattgaag accagcgtca
    36361 tgtactatct tctatactag atatcgcgaa agaagaaaaa gtagaggcaa tcgtgattgc
    36421 cggagactta tatgatagga gtgtcccagc tgttgatgcc gtagaattat acaatgaatt
    36481 gatggtcgat tggaatttaa aggaaaagat cccgattttt gctatatcag gaaatcacga
    36541 tagcagtacg agactttcag cgggtacgcc atggttcagt tattcgaact attttttgcg
    36601 aacacagcta gcagaagcat ttgaaccagt cgagtttaaa aacgtacaat ttttctttct
    36661 tccttacttt gaaccaattg cagcgagatt atattttgaa gatgacaata tccgaacgat
    36721 caagcaagcg gtagccaagg tgatagataa aatgcaggag ctatttgatt cagaaaaaaa
    36781 acaagtattg attagccatt tttttgtaat gggaagtgta aaatctgatt cggaaacgaa
    36841 aatcgaagta ggcgggttgg attcgatccc tggtgaacta cttacaccgt ttgattatgt
    36901 agcactagga catttgcata atcaagctgc tttgagacag gataatgctc gctatagcgg
    36961 ttccccattg aaattctctt tatctgagat caaccaaaaa aaaggcgtat ggatcgttga
    37021 cctgacaaca ggcctggagt tgaattttag ggagatcgaa cccctgcatg atattgaaga
    37081 aataacagcg agtttcaaag aattgctgga tcctgccttt tatcagtccg tcaatcggga
    37141 gaattacatc tatatctatc tcactgatag aacgatcatc ccgaatatga tgaaccagct
    37201 tcggcaaata tatcctcgta ttttggggat tgaacgggca aacggtcgag cagaacaaac
    37261 aaagcgcaaa cgaaaagtac tagaaatgaa agatcctcaa aaattagcag aagcattttt
    37321 tagtgaaatg acggaagagc agctaactgc aaaccaagaa aaatggctcg gagaaacgtt
    37381 gcaagaaatc atggaaatgg aatgatcaag aatgaaaccg aaaaaattac aacttaaaaa
    37441 ttttggtcct tttatcaatg agaccgtaga tttttcacgt ttgacagaag caccgctttt
    37501 tttgatcagc gggaagacag gagctggcaa aacgacgatt tttgatggga tcaccttcgc
    37561 actttttggt gaaacttccg gaagacttcg ctctggaaaa gagatgcgtt ctttgtttgc
    37621 tacaccagac gaagaaacaa gcgtgacttt ttcttttgaa catcagcaga tgagctatga
    37681 aatcgaacga aaaccagaac aagttttagc aaaacgaaaa ggaaacggaa caaaaaaaca
    37741 ggcagccaaa gtaacgttga ccatttttga tgggaaagga aaagaaataa aacaaatcac
    37801 aaaacgtaca gaagcagatc agttgatcag agaactgatg aatctcgatg cgaaacagtt
    37861 ttctcaaatc gttttactac cccaaggaga attccgtaat tttttggttt catcaagcag
    37921 tgataaagag actgttttac gtcatttatt cggtactcaa tttttccaac agttcaatga
    37981 gcgattaaaa gaaaaagcaa aacaacagca acaaaaattg gatcatttag agcaagagct
    38041 gcatcttctg caaaaaaggt ttgttttagt caaagaagag gaaccagcag cagggtttga
    38101 agccgtttta gcccaatgga caaaccatca ggcaatactc gaaaaacaaa tcacggaaga
    38161 aaaagaagct ctaacggaac ttcaggaaaa acaaaagcaa ctagaaaatt cctactatcg
    38221 ttttgaaagc ttgaaaaaaa gttatgaaga aaaaacgata ttaataaaca agcaaaagga
    38281 attagctgct caaaaagacg aaatagagga aaagaaacga tggattgggt attatgaatt
    38341 tacgcaacga ttagtcgaac caatccagcg cctacaagaa ataaaagttg aaaaaataga
    38401 actgtcacaa aaaagtagtg agcaaacaaa gaaactagcg atgactacgc agaattatga
    38461 agagtggcaa aaaaatgaag caaaccgcgt acgccgacaa acaaaaatca aagaagctgc
    38521 tcaaaaactg caaaacaaac agatgatggt gccaattgtc gaatccttac gtagtaaaaa
    38581 acaagacgca gaacaattaa caatttcatt ggataaagaa gaattgaatg ccgagaagct
    38641 gtatcaaaat ctcgctatct atcaggaaag aactcgaatt cttcaagaaa atttactgga
    38701 tcaggaaaaa ctgcaagaag atcgtcttgc ttttggacag ttaaaatatc tagatgaaaa
    38761 gcagcaagaa atagaaaaac aaaaagcaaa agacactggg tcgctcgcac agataagaga
    38821 agaactcaaa aaagcaactg aagaaataac aaaaaatagt cagctattga aagaacagac
    38881 agaacaagca gaacagctga aaagccaatg ggcaaagcag caaattgctc gtcttcagct
    38941 ttttttgcgt ccaggggagc catgtcttgt ttgtggatca acggaacacc ccatacaaca
    39001 attaaaacac gaacagccaa ataaggaaga tatcttaaaa gtagaacaac aactggcaga
    39061 agctgagtct gctgtagaaa gaactcagca aatcattgcc aaaagtgaag cacatgaaaa
    39121 taatcttcaa caacaattat cagatttaga acaaaaaatc atggcaacga atatacaatt
    39181 ggctgaacaa gaagaacact tgcaacaggc tttgaaaaag gcatttcctg agataacaga
    39241 acaaaaaaca aaaacaactc ttgtagtaat cgaacaatct ttagatagac gaaaaagcaa
    39301 gatggccaat gcaagaatag agctggaaga aataagacaa caagcagaaa agctcgaaca
    39361 acaattagct gtctacaaac agcagcagac agaaaaaaat aatcagctcc agcaactgca
    39421 aggagaaatc aaaagtttag aacaacaatt gcaagatacg gatagtaact gtttagaaga
    39481 ggagatacga cgacttgaaa aacagttaga agaagactcg aatcaactag ctgttgagca
    39541 gaaagcagga gtgcaattgt tgcaagatct tcatacgctt cggacacagc ttgatcttca
    39601 acatgaacaa atagaaaaaa tagatgaaaa aatagaaaag gaacaagaaa gaatagctaa
    39661 aaagttagaa agccagtcgt atttttcaac aattgaagaa atctgtgcat ttgcacaaca
    39721 gcaagcacaa tataaagaaa accaacgtac agttacttct tatcatgaag agcaattagt
    39781 gacagctgac cggctgcgtc aattagaaaa tatccagcta actgagacat tcccagatac
    39841 agaagagatc agccaacagt tagaagaaat aagacgtatg atcgaaataa agcaagaaaa
    39901 gatatacggt ttacaagaac aaaaaattag taatcaaaaa atctacgatg aatgtctttc
    39961 gatttatcac acaagccaga cgaaattaga tgagcttacg caattacagc agctttcgca
    40021 aacattcaac ggagaaaatc ctaagaagac tagtttggaa cggtatgtgc ttcaggttta
    40081 tcttcaagaa gtgttacaag tagcaaatga tcatctccaa cgtttaacga aaaatcgtta
    40141 tcaatttgag ctagcggata ccattggcag ttatcgaggg aaaaccggat tagaaatcaa
    40201 tatctatgat gatcaggcag gtatgtctcg aagtgcgcat accctgtctg gcggtgagag
    40261 ttttattgca gcactttctt tagcattagc tcttgccgat gtgatccaga cacaagctgg
    40321 tggtgtagca atcgatgcat tgtttattga tgaaggattt gggtcattag atgaagaagc
    40381 tttggaaatg gcaatggaag ctttagaaac gatcgaaagt gaaggaagaa tgatcggcat
    40441 cattagtcat gtaagagaat tgaaagaacg agtattacaa caaataagaa ttgatacaga
    40501 aggtagcgga caaagcaaaa tacgctatca acttggataa aaagggagat tggtaactat
    40561 gaaatggagt attccagaaa aagtcattgc acgtggacgt caatacttgc aagaaggccg
    40621 agtgttatcg gttgtccctg accaagaaga aaacgtgtgg catgcagaag tcttgggcag
    40681 tgaattgtat atggttgatt tggatggtac agcaaaagag atagattact gccaatgtcc
    40741 ctattgggag gaacataaat attgtaagca tacagttgct gtggaacttt atttacggtc
    40801 gaaaaatcta tctcgtatca tgaaaaaaga ccagccaatg ctagaaccgg taaaagcaac
    40861 aagtgaggga gaaatcattg caaaaggttt ttctcgtcta aaaacacaaa acaatcctca
    40921 agcggtgcaa cctttgatta ttgaatacca agtagaaacg attgagacta atcagtatca
    40981 cccagagctt tctattttgg cactttactt gcgtatcggt tcattggcat caccgaaaaa
    41041 aacatatatc gtaaaaaaca tctatgattt tttacaagct tacacagaaa aagaaactta
    41101 cactgttaat aaacagtacc agtttcgtct agataaacgg gcgtttcaaa aagaagatca
    41161 aaaaatactc acgcatcttg caggaagtgc tcagacgcat caattattag gtacgaacgg
    41221 cttacaagtc aaaggaaaac tagataaaag atatctgctt ctcccagtag aacaagcccg
    41281 cttcctgtta gagcagatga atcagactac acgtttgcat ctgcagataa atgaacaaaa
    41341 cgatcaagga atacaatttt ccgatcaaaa caaaccttta tcattcaaag tggaaaaatt
    41401 tggagaagat tatttgctgc aagcattgaa tgattttgat tttttcttta tgcattatca
    41461 gtggggagtt atccaaggga cattttattc tctgacgaaa tatcaacaaa cggtttatca
    41521 aacttggaaa caattgattc gccgattgga gaaaccagaa gtagtgttca agaaaaaaga
    41581 attaccgtct ttgtttaaag agattatccc tctgctgtca gagatcggcg acgtagctat
    41641 tgcccctgca gtagaagagg aagtagcaga tatccccgtc gaatttattt tctttttccg
    41701 aaaagcaaaa ggaaaaatcc aagctcgagt cgatttcgtc tatggtgaag tcatctattc
    41761 gactgacccg aagcatgagg tctcgacaga acaggctttt caggtgttac gtgacacagt
    41821 agaagagaaa cgagtacttg atctattcag aatgtatcgc taccagaaaa atgaaactgg
    41881 ttatgaacgt ccattgccaa gcggggaaga actatatgcc tttttccgca cagaactatc
    41941 tgtcttccgt cagtttggtg aagtcagact tggaagaaaa ttgcgagagt tgtatttaga
    42001 tgcgcataaa catcaaccga aaatcgaagt aaccgagacc ggttcatggc tcgatattcg
    42061 attcgatgtg acagggatag aggagcaaga aatcgatcat gtcctgcaaa gcttgctgag
    42121 aaatgatgct ttttatacat tagaaaatgg tcaggttctt tcttttgatt cagaagaatt
    42181 tcaacaaaca agccaagtgt tgcaacaatt acgggaatcg atacgtacag aagaaggaac
    42241 gatccatgta cctaaaaatc aaggattgat cattcaaaat caattggaaa aaagcaatgc
    42301 tacttttagt gaatcgttcc aaacgatggt acaagatctc attcatccag aaagatacca
    42361 ggcacagctt cccaaaggat tgaatgcaac gatgagagat tatcaaaagc aaggttttcg
    42421 atggttgaag atgttaggtc attatcagtt tggcggtatt ttagccgatg aaatgggact
    42481 tgggaaaaca ctacagacaa ttgccttttt actttcggaa aaagaagaac ggaaaagctt
    42541 ctctgctttg atcgtagcgc ctgcgagctt aatttacaac tggcaggcag aggtgagaaa
    42601 attcgcccct tctttaagta tccaagtaat caatgggaat aaaaaagaac gtgaagaatt
    42661 actggctaaa gacacagata tccgagtgac ttcttatgcc agcttaagac aggatctagc
    42721 taattatcag tcgcaaaaaa ttgattattt gattcttgat gaggcacaaa tggtcaaaaa
    42781 tagcagtacg aaaactgcac aagcattgag agaattagct gtacctcagc ggtttgcact
    42841 gagtgggaca ccaatcgaga ataatttaga agaattgtgg tcattgtttg caacgattat
    42901 gccaggattt ttcccgacta aaacaaaatt cagggaactt gccgcagaag aaattgcgca
    42961 aatgattcga ccgtttattt tacggcgaga taaacaaact gtgttgaaag acttgccgga
    43021 aaaaacagag atgaatctat attctgcact tacagaagaa caaaaaactg tttatttagc
    43081 ttacttgcgt caaatgcgtg aagaaatcac atcaatggat tctgaagcat tcaagaaaaa
    43141 ccggatcggg atacttgctg gtctgacaag actgcgacaa atttgctgcg atcctcgtct
    43201 gtttattgaa gattaccaag gcggttccgg caagctcgaa caagtaaaag atctactggt
    43261 agctgccaaa gagaacaaac gaagagtttt gctgttttct caatttacag ggatgctgac
    43321 tatcttgcaa gaagagttag ccgagctagg gatctcgaca ttttatttaa gaggcagtac
    43381 gaaaccacaa gatcggttaa gtatggtaga cgcatttaat gctggcgaaa aagatgtttt
    43441 tctgatttcc ttaaaggctg ggggaactgg attgaatctc acgggagcag atacggtcat
    43501 cttgtatgac ctttggtgga atcctgccat tgaagaacaa gctgctggac gtgcacatcg
    43561 gatcggacaa aaaaatgtgg tagaagtgtg gcgtatgatc tctgaaggaa cgattgagga
    43621 acgcatggat tcactccagc aagaaaaacg cgaactgttc caaaaagtga tccaaggtaa
    43681 tgaagaacag ttgacgaaac tgactgaaga agacatccgc atgattttaa gtgtaggaga
    43741 aatcgaggag taagagactc gaaaaagcgt tcagtaatgg gtaagaaagc ctttctaaag
    43801 aagcgattgt ttatttgatt taaaaaatag caaggaggct gggattccat tttatcccag
    43861 cttccttgct atcatttttt tatttctatt tttaaaggtt atacaaagat tgtcttcatt
    43921 gtctctaaca gctgatcaaa agaagaaatc tcgatatcgc aaattggagt ctgttcaatt
    43981 ttgcgttctc gatggttgaa ccaaagcgct tgccagttag cactttttgc acctagaaca
    44041 tcattgtcgt aattatcgcc aacgtataag gtctcttctg gaagcatatg aaattctttc
    44101 tcagccagct gaaaaatctc tttttctggt ttttggaaac ctgttgcttg agaaatgagc
    44161 atattgcggg aaggaatcca attgttcaat tgaagttgct tcagtttttt tgtctgatgg
    44221 tcagtcggcc catttgtgat aattcctaat gggacattct tttccttcaa gaaatcgagg
    44281 gtttttttga cctcctcatg caagcagata ttatcaagtt cttcctcgta aattttttga
    44341 aataagaggc catcttcttc cgtaatatga gggtagtcta aatcttttag tgattggctg
    44401 atccgatgtg ctcgcatata ttctagtgtc caatctcctg ccattacttt tgggaagttt
    44461 tcatcactat gatggcggaa ccggatatat agtggatgca tatctgcatc tgaaacaagc
    44521 ggaacgactc ttttgaccgc gtttcgaaat ggctgctgct gatcatacat cgtatcatca
    44581 acatcaaaca caactgattt aatcaaaatt ttacacgcct ttcggttttc attgattttt
    44641 tcacaaatcc ttgactattg taacagaaaa aattgtctaa aaaagctgat aaataaaggt
    44701 ttttcttact atatttctat ttccttgaca gaataaggtt tttatatttc agtttttttc
    44761 actaaaaatc gtgaaacacg tcaataagtt tgatgaacaa attaatcatt atggtagaag
    44821 tcgattgatt tattttaatg caatctctgt ggaattttcg aaagatttat ttttttaata
    44881 cagtctcaat ataactattc aaacataa
//
LOCUS       DAYYBG010000015        35438 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000015 DAYYBG010000000
VERSION     DAYYBG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.015"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACHAUN_000818"
     CDS             <1..1152
                     /locus_tag="ACHAUN_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFM8459542.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHAUN_000819"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHAUN_000819"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8459543.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACHAUN_000820"
     CDS             2728..3003
                     /locus_tag="ACHAUN_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8459544.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACHAUN_000821"
     CDS             3009..3776
                     /locus_tag="ACHAUN_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8459545.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACHAUN_000822"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACHAUN_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8459546.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACHAUN_000823"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACHAUN_000823"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8459547.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACHAUN_000824"
     CDS             7920..8222
                     /locus_tag="ACHAUN_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8459548.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACHAUN_000825"
     CDS             8225..9388
                     /locus_tag="ACHAUN_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8459549.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACHAUN_000826"
     CDS             9414..12842
                     /locus_tag="ACHAUN_000826"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8459550.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACHAUN_000827"
     CDS             12863..13987
                     /locus_tag="ACHAUN_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8459551.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACHAUN_000828"
     CDS             13968..14414
                     /locus_tag="ACHAUN_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8459552.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACHAUN_000829"
     CDS             14398..14736
                     /locus_tag="ACHAUN_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8459553.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHAUN_000830"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHAUN_000830"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8459554.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACHAUN_000831"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACHAUN_000831"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8459555.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACHAUN_000832"
     CDS             15893..16936
                     /locus_tag="ACHAUN_000832"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8459556.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACHAUN_000833"
     CDS             17095..17823
                     /locus_tag="ACHAUN_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8459557.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACHAUN_000834"
     CDS             17939..18184
                     /locus_tag="ACHAUN_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8459558.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACHAUN_000835"
     CDS             18181..18399
                     /locus_tag="ACHAUN_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8459559.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACHAUN_000836"
     CDS             18535..19074
                     /locus_tag="ACHAUN_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8459560.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACHAUN_000837"
     CDS             19067..21340
                     /locus_tag="ACHAUN_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8459561.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACHAUN_000838"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACHAUN_000838"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8459562.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACHAUN_000839"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACHAUN_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8459563.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACHAUN_000840"
     CDS             complement(23167..23436)
                     /locus_tag="ACHAUN_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459564.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACHAUN_000841"
     CDS             complement(23698..23826)
                     /locus_tag="ACHAUN_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459565.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACHAUN_000842"
     CDS             complement(23906..24361)
                     /locus_tag="ACHAUN_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8459566.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACHAUN_000843"
     CDS             complement(24499..26427)
                     /locus_tag="ACHAUN_000843"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8459567.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ACHAUN_000844"
     CDS             complement(26624..27457)
                     /locus_tag="ACHAUN_000844"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8459568.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ACHAUN_000845"
     CDS             complement(27649..28353)
                     /locus_tag="ACHAUN_000845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8459569.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ACHAUN_000846"
     CDS             complement(28376..29002)
                     /locus_tag="ACHAUN_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8459570.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ACHAUN_000847"
     CDS             29328..31703
                     /locus_tag="ACHAUN_000847"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8459571.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ACHAUN_000848"
     CDS             complement(31727..32218)
                     /locus_tag="ACHAUN_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8459572.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ACHAUN_000849"
     CDS             32405..33325
                     /locus_tag="ACHAUN_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8459573.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="ACHAUN_000850"
     CDS             33479..33823
                     /locus_tag="ACHAUN_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8459574.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="ACHAUN_000851"
     CDS             complement(34013..34513)
                     /locus_tag="ACHAUN_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8459575.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="ACHAUN_000852"
     tRNA            35041..35124
                     /locus_tag="ACHAUN_000852"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11775 a         6171 c         7295 g        10197 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gttttttccc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DAYYBG010000016        34961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000016 DAYYBG010000000
VERSION     DAYYBG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.016"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..1863
                     /gene="efbA"
                     /locus_tag="ACHAUN_000853"
     CDS             157..1863
                     /gene="efbA"
                     /locus_tag="ACHAUN_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8459576.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2021..2923)
                     /locus_tag="ACHAUN_000854"
     CDS             complement(2021..2923)
                     /locus_tag="ACHAUN_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8459577.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2920..3612)
                     /locus_tag="ACHAUN_000855"
     CDS             complement(2920..3612)
                     /locus_tag="ACHAUN_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8459578.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3655..4296)
                     /locus_tag="ACHAUN_000856"
     CDS             complement(3655..4296)
                     /locus_tag="ACHAUN_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8459579.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4334..4648)
                     /locus_tag="ACHAUN_000857"
     CDS             complement(4334..4648)
                     /locus_tag="ACHAUN_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459580.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4636..5265)
                     /gene="pyrE"
                     /locus_tag="ACHAUN_000858"
     CDS             complement(4636..5265)
                     /gene="pyrE"
                     /locus_tag="ACHAUN_000858"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8459581.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="ACHAUN_000859"
     CDS             complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="ACHAUN_000859"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8459582.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5985..6917)
                     /locus_tag="ACHAUN_000860"
     CDS             complement(5985..6917)
                     /locus_tag="ACHAUN_000860"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8459583.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6914..7690)
                     /locus_tag="ACHAUN_000861"
     CDS             complement(6914..7690)
                     /locus_tag="ACHAUN_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8459584.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7744..10929)
                     /gene="carB"
                     /locus_tag="ACHAUN_000862"
     CDS             complement(7744..10929)
                     /gene="carB"
                     /locus_tag="ACHAUN_000862"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8459585.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10934..12010)
                     /locus_tag="ACHAUN_000863"
     CDS             complement(10934..12010)
                     /locus_tag="ACHAUN_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8459586.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12015..13295)
                     /locus_tag="ACHAUN_000864"
     CDS             complement(12015..13295)
                     /locus_tag="ACHAUN_000864"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8459587.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13314..14240)
                     /locus_tag="ACHAUN_000865"
     CDS             complement(13314..14240)
                     /locus_tag="ACHAUN_000865"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8459588.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14253..15536)
                     /locus_tag="ACHAUN_000866"
     CDS             complement(14253..15536)
                     /locus_tag="ACHAUN_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8459589.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="ACHAUN_000867"
     CDS             complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="ACHAUN_000867"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8459590.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16324..17235)
                     /locus_tag="ACHAUN_000868"
     CDS             complement(16324..17235)
                     /locus_tag="ACHAUN_000868"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8459591.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17232..17696)
                     /gene="lspA"
                     /locus_tag="ACHAUN_000869"
     CDS             complement(17232..17696)
                     /gene="lspA"
                     /locus_tag="ACHAUN_000869"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8459592.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17701..18399)
                     /locus_tag="ACHAUN_000870"
     CDS             complement(17701..18399)
                     /locus_tag="ACHAUN_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8459593.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18386..18772)
                     /locus_tag="ACHAUN_000871"
     CDS             complement(18386..18772)
                     /locus_tag="ACHAUN_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459594.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18919..20589)
                     /locus_tag="ACHAUN_000872"
     CDS             complement(18919..20589)
                     /locus_tag="ACHAUN_000872"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8459595.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20772..21005
                     /locus_tag="ACHAUN_000873"
     CDS             20772..21005
                     /locus_tag="ACHAUN_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8459596.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21104..21535)
                     /locus_tag="ACHAUN_000874"
     CDS             complement(21104..21535)
                     /locus_tag="ACHAUN_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8459597.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21614..22027
                     /locus_tag="ACHAUN_000875"
     CDS             21614..22027
                     /locus_tag="ACHAUN_000875"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8459598.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22065..23213)
                     /locus_tag="ACHAUN_000876"
     CDS             complement(22065..23213)
                     /locus_tag="ACHAUN_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8459599.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23335..23931)
                     /locus_tag="ACHAUN_000877"
     CDS             complement(23335..23931)
                     /locus_tag="ACHAUN_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8459600.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="ACHAUN_000878"
     CDS             complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="ACHAUN_000878"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8459601.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27161..27592
                     /locus_tag="ACHAUN_000879"
     CDS             27161..27592
                     /locus_tag="ACHAUN_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8459602.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27637..28986)
                     /locus_tag="ACHAUN_000880"
     CDS             complement(27637..28986)
                     /locus_tag="ACHAUN_000880"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8459603.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29056..30219)
                     /locus_tag="ACHAUN_000881"
     CDS             complement(29056..30219)
                     /locus_tag="ACHAUN_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8459604.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30348..30671
                     /locus_tag="ACHAUN_000882"
     CDS             30348..30671
                     /locus_tag="ACHAUN_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459605.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30854..32203)
                     /locus_tag="ACHAUN_000883"
     CDS             complement(30854..32203)
                     /locus_tag="ACHAUN_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8459606.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32272..33225)
                     /locus_tag="ACHAUN_000884"
     CDS             complement(32272..33225)
                     /locus_tag="ACHAUN_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8459607.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33327..34517)
                     /locus_tag="ACHAUN_000885"
     CDS             complement(33327..34517)
                     /locus_tag="ACHAUN_000885"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8459608.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34717..>34961)
                     /locus_tag="ACHAUN_000886"
     CDS             complement(34717..>34961)
                     /locus_tag="ACHAUN_000886"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459609.1"
                     /translation="RKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVF
                     LLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9951 a         7642 c         6120 g        11248 t
ORIGIN      
        1 tacctaaaaa tttggatttc aacgatagaa acactgtttt ttatgtaatt agtccaagat
       61 ttttctagac ttttgcttta ctattcggcc aaattggttt ttcttctttt tttcgttata
      121 atgaatatac ttatgaaaag aaatgaggaa tgaaccatgt cctttgatgg tgtatttacc
      181 cacgcgatgg tcaacgaatt gcgtgaaacg ttactatctg gccggatctc gaaaatccat
      241 cagccttatg aaaatgaaat cgtgctggtg attcgttcac gcggcaaaaa ccatcgatta
      301 ttattatctg cgcacccaag ttatgctcgt gtacagatta cccaaatcga ttatcagaat
      361 cctgataatc cgcctaactt cgtgatgatg cttcgaaaat atttggatgg cgctatttta
      421 gaagacatcg aacaaatcga aaatgatcga gtgatccatt tccactttgc taaacgaaac
      481 gagttgggtg atctacaaaa tatcatattg atcgttgaat taatgggacg tcatagtacg
      541 atcgtcttag ttaatcgaga aactggaaaa atactggatg cgatcaaaca cattggaagc
      601 tctcaaaaca cttatcgttc tttgcttcct ggtgtagaat atgttgctcc gccaaaacaa
      661 gaggtcttga acccatttag ctccgagaaa gaaaaaatct ttcagcggct ttcccaaact
      721 gaactagatc caaaagcaat ccaacgtcag tttcagggaa tcggtttcga tatcgctcaa
      781 gagctgacca aaagattact ggaacgtcct aatgaaaaga tggttgtatg ggacgaattt
      841 ttctcagcca tcagccatca gcctattcct actttttacg aaacggaaaa taaagatttt
      901 ttcacaccga ttgcttatca ggtattgtcc gaacaggcat ctgctgtaac agcttatcca
      961 acactcagtc aattattaga tagctattat cacgaaaaag cagagaaaga tcgggcaaaa
     1021 caacaaggcg gagaactgat tcgaaaaatc gaaaatgaac taaaacgaaa taaaaataaa
     1081 ttgaaaaaac gtgaacaaac actcaaagaa tccgaaaacg ctgagaatta ccgtagaaat
     1141 ggcgaactac ttactacttt tctgacacaa gtaccacgcg gcgcaaaaga agttgtttta
     1201 cctaattatt atgaagaaga tcggccaatc aaaatagcac tagatccggc attgacacca
     1261 aatcaaaatg cacagaaata tttccatcga taccaaaagc tgaagaacgc tgttaagctg
     1321 attggcgaac aaatccaaga agccaaagat gaaatccaat atcttgaatc tgtcttatct
     1381 caactggaaa ttgccggccc aatggatatc gaagcaatta aagaagaatt gacttcagaa
     1441 ggatacctga aaaagaagaa cttaaaaaaa caaaaacgta agaaaccatc tcagcctgat
     1501 caatactttt cttctgacgg aacgctcatt cttgttggta aaaacaatct tcaaaatgac
     1561 cagctgacaa tgaagacagc taaaaaaacc gactattggc ttcatgcaaa aaatataccc
     1621 ggttcgcacg tcatcattaa aagtgacaag ccctcagatg aaacaattac agaagctgcc
     1681 gagttagctg cctatttttc aaaataccga tactctgccc aagtgccggt tgatcttgta
     1741 caggtaaagc atatccgaaa accaaatggc gccaagcctg gttatgttat ttatgaaaac
     1801 caaaagacga ttatcgtgac accagaagaa gaaaaaatta cagctatgaa acaaaacagg
     1861 taatcaatga gtgtttctag aatcatattt gcgtactttc ccacaacagc aattagttat
     1921 aaattgtttg ctacaaaaag aggagttgtg acaaaattct gtcacaaccc ctcttttttg
     1981 atcaacggtg ttcaataaac agcgttctca tctgcttttc ttagctctgc tggtccagta
     2041 accgagtaag ctctttgata aatctttgag cgatttcact gagcggacgt ttcgccgaat
     2101 aaatgatata aaattcattt agttcgcctt ctgccaaagg gattaaccgc aagtgctcgc
     2161 gaatccctcc aatcacaata ccagatcctg aagcgtaagc atcagtttca agaacgatat
     2221 tcatcaaagt acctcgatca ctcgtataaa tcactgtttc ttgatcaggg atctcgataa
     2281 ggtcttcata aaaatacggg aaattatttc catcctgtgt aaaacgaact tgaggataag
     2341 ataacaattc ttctttggtg atctctgcgt gttcagctaa tggatgtcct tgccttaaaa
     2401 aaatatgcgt tttgaatttt ccaatgctgc ggccacttag ctctccttgt tgtaaatatc
     2461 ttctgatacc agcttgatta tgctcattca tataaataat gccgatctca ctgtgctgct
     2521 ctttgacttc ttcgatcact ctcgctgttg tcgtctccat gatccgaaat tgtttgatct
     2581 cgtctttgaa ttcctcgagt agctgtgcgg tcaaaggacc taagaaatca taatgctggg
     2641 cagcgatcga aaaattgtct ctcttttcac tcgttagata gtgagtttct aatgtatcaa
     2701 gctggaccaa tattttctcg gcacgattta agaagtcttg cccttcttct gttaaatatg
     2761 cccctcgatt cgttcgagta aacagcgtga tccctaactc ggtttctaat tctttgatac
     2821 cagctgaaag acttggctgg gagacaaaca ttttctttgc cgcttctctg aaactaccat
     2881 tgtttgctac agcgaccacg tatttcatct gctgaatatt cattgctcaa actcctcgaa
     2941 ataataaatt tctcttccat tcaagggctg gattggtcgg ttgttcggtc gagcatactg
     3001 accatcgaag gggagaagaa actcttggtt cagttttcct acatgatcca ttacaaacca
     3061 ttggattggc ccgctggtag gcggagttgt caatgagcca atataatgga aatggctttt
     3121 ttgctccgga aagaacacat ccggatttac ccaatgctca tgtttttcta agttccaact
     3181 ttccccattt tcttgattat acagccaacc ttcctgtacc attagctggt acatgacacc
     3241 cacaactaga ttctcttttt tgctgttcat atgaacaaaa tgaaattcaa tatcttcctg
     3301 ttttccgttg atgatatgtt cagaaggcaa gtggaaatga atatctgtca aataatagcg
     3361 ttctttcttg aactctacat aactgagttg gtcaaaaggt actaaatgaa tcgtgttttt
     3421 gaattccttg tcagtaaacc gttgcttttg ataatgaaag gaaagcgtgt ctccttctat
     3481 tttggtttct tcccccttgg tcaaatgaat aggcgactgc aatggaaatt ccgcacctct
     3541 cgcaaaccaa tcacataaac tcgcccaatt ttccggacct ttgtctccag aatagcccca
     3601 ttcgacatcc atattctttt tcaactatac ttccccctgc atctcatatc atttttattt
     3661 ttcttctttc tttacaatcg ttgagacggt ttctaaaaac gcctcttttc cataaaattc
     3721 ctggttcaat agttccggaa atatctcttt aaagaaatac tgaacagaag gccgatgttc
     3781 aaagctttta atcgtagtca ctgcctcaat gaacatctcc ttataaactt tttccgggtt
     3841 atttttgatg atttcatcaa aagattcata aagaaggtca accttatctg aaatggctaa
     3901 tagttctcct tctacggttt catcttttcc ttcgaacaac cgacggcgat aaacttcttg
     3961 gaactcagct ggaatctctt ggttgatgaa ttcgtccgtc atcttttctt caactgtttg
     4021 cagcatccct cttaactctt tgcttgcgta tttcaccggc gttttgatat caccaataaa
     4081 acgctctgtg tagtcatgat tcaatgattt ttcatagagc agtttccaat cgatcggttg
     4141 tccattcgct tcttcaatgt ctcctagaac ttgcgcaata gatgctacac gatacgaatg
     4201 tgctgcaact gtatgctctg tatatttaaa aaatccaggt gcccttgtga tcgtctctaa
     4261 attgttcaat cctaaaataa attcattcaa ccccataaaa gttacctccc tttgagattt
     4321 ctttttccga atatcatatc attaaataac tgatattcgc aacatttccg ctctttacca
     4381 tttttgaaac agctccctta ccaaaaaata gagaaaatct atatataata gaaaaaagag
     4441 aagagattat tctcttcttt ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca
     4501 aaaactgatc acgatagaag tttcttttca tacctgatga aaagatgctt ctcgcaatca
     4561 gtttttggat ttttgctatt ccaattgatg attctggaga cacaaatcgg atttctctat
     4621 cttcatcaag cgcgcttaaa tctgccaatt ttcaggatct tttttccatt cttttagcag
     4681 agttaattct tgctcatcga tataacgatc ttctaaagct gcttcaatca atacagaata
     4741 attggtcaac gtcataagag ggatttcggc tttttcgaaa tttgcttttc cttttggcaa
     4801 ctcatacgta aagatagccg caacaccaag gatatccgca ccttctcttt ttgctgcttc
     4861 agctgcttcc aagacacttc cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg
     4921 accttcaaat atacgtcctt cgatttggtt acctttccca tgatctttcg ccttacttcg
     4981 aatatagacc attggcaaat ccagtatttc cgctacccat gcggcatgag ggattcctgc
     5041 tgttgcagtt ccagcaatca cttgtacttc agggtaagct tctttgattt tgcttgctaa
     5101 tccttttgcg atctcttttc ggacttttgg atagctcatc gtgattcggt tatcacagta
     5161 aattgggctt ttgattccac ttgcccatgt aaaaggatta cttggattta aaaatactgc
     5221 ttcaatttct aataagtctt ttgcgatact ttgttcaaca ttcattttag gttagctcca
     5281 ttctgtttga atcgtttgat atgctttata aggattagtt gactgagtga ttggtcgacc
     5341 tataacgata aaagttgagc cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg
     5401 atctcctgcg actgttcctt ctggtcttat acccggagtt aagcaaacaa attcgtctcc
     5461 tgccatttct ttgatcgctt ctacttccca agcagaagaa accacaccat ctaatcctgc
     5521 ttttttggca cagctagcat aatggatcac acttttttct aaagaaactt cgattagttg
     5581 atcggcatgc atttcttctt cacttgtaga agtaagctga gtgatagcta gaagtagcgg
     5641 acgttttcca ccttctggag tcccctcttc taatcctcgc attgccgcct ccatcatacg
     5701 aataccgcca gcggcatgaa cacaggtgag atcaacacca agctttgcca atccccgcat
     5761 tgctttttcg accgtattcg gaatatcgtg aagtttcaga tctaaaaata cattatgacc
     5821 agcctctttt agataacgta cgatttcagg tccttcttgg tagaacaatt ccattccgat
     5881 tttcacaaac aatttttctt ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct
     5941 agttgaaaaa tctaatgcaa taattggtcg ttggtctagt tgattcatcg ttcacgttcc
     6001 tctctcactt ctttgattaa tctttccagc gattcgatgc ctagttcgct cattcgcaca
     6061 gggagctctt taattagttt aggacagata tacggatcgg tgaaattcgc ggtccctata
     6121 gctacagcac ttgctccagc cataaacatc tcaagaacat catccactgt ctgcacgccg
     6181 cccatcccaa tgattggaag gctggatata cttgctactt ggtgaatcaa acgaatagcg
     6241 acaggcttga ttgctggtcc ggagagtcca cctgtttgat tggctaaaat cggttttctt
     6301 gtcttaaggt caatccgcat accaagaaga gtattgatca tcgtaaaccc atctgcccct
     6361 ccagcttcga ttgcctgagc gattggaacg atgtctgtta catttgggga caatttgaca
     6421 tagacaggaa cagaagaaac tttctttact gcttgtgtta attgaaaagc aacatcagaa
     6481 tctgttccaa aagcaattcc gccatgtttg acatttgggc aagaaatgtt cagctcgatt
     6541 gctttcacat ttggtgcttc gccaattttg ctacaaactt ctacatagtc ttcttcacaa
     6601 gcaccagcaa catttgcgat gatgggtaac tcattgtact cctctaattc tggaagaagt
     6661 ttatgcataa caacgtctag accaggattt tgcaaaccaa ttgcattcaa catgccagat
     6721 ggtgtttccg ccactcttgg cgtgggattg ccaaagcggg catttggagt cgttgccttg
     6781 accatgatcg atccaagctg attcaaatca tagtattttc cgtattcttt gccaaaaccg
     6841 aaacagccgc ttgccggcat gataggattt tttaattcta ggccaggaag tttgaccgct
     6901 aaaggattct cattcatata attacctctc ctattttaaa aatcggtcct tcgtcacaaa
     6961 ctttcacact ttttgttcct gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag
     7021 cccctatccc acatgccatg cgggattcta atgataactg gacattttga tgcgaagaaa
     7081 acagctgttc cactgtcttc aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt
     7141 ccgtcttcgt tcctaacaat aaattaccga catttccgtg cataccgtat gttccatcgt
     7201 cagtagcgat tcttgtttct cctagacgat taaattcttt ttcataataa acgacttcat
     7261 gtgtggcaaa acctaaaaaa tgcgttggct taattccttt ttcgactaac tgttttgata
     7321 actcatatag aggagggaca ccgattcctc caccgattat aaatacttcg tctcctgtat
     7381 gaagattgga caaatcaaaa ccattgccta aaggacccat gacatctaaa tggtctccgg
     7441 cagaaagagt agagaagact ctagtcccct caccttccac ccgataaatg atacggcaag
     7501 tatttgtttc gtggtcgata tgattcaagc taatcggtcg gcgtaatagc aagtctgcac
     7561 gcggcacgag aatatgcaag aactgtcctg gttgctgcat ttcttttact agatcacctt
     7621 tcaatgtcat ttcaaaaatc tttggtgcca gctgtttttg ctgaacgatt tccatcattt
     7681 cttgtttcat ctgtcccctc ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg
     7741 ccgttaaata gggttagttg aaaaagcacg ggattccatc actcgaacga tcgcatctgc
     7801 tgtgtctaaa gaagtaaaca atggaatacc atgttcaact gcttctcttc ggatgatgaa
     7861 accgtcttta gaagcatttc cccgatcttt atcaatcgta ttgacaacca cttgtgtttt
     7921 accatctctt attaagtcga ctacattttt ttctgctgct tcctcagaaa ttttcgctac
     7981 tgtttcaacg accagaccat ttttttcgaa ataagcagct gtatgtttcg ttcctatcag
     8041 actatagcca atttctgtaa aacgtttagc taattctagt gcttcttctt ttgtttcgtc
     8101 cgcaatcgta aataatacgg ctccatattc aggcaagtgt aatccagaag cttcgaatgc
     8161 cttgtacaaa gctttgtcta attgtctgtc agatcccatt acttctccag tcgatttcat
     8221 ttcaggccct aagtaagtat caactttctg caatttcgta aaagaaaaga caggtgcttt
     8281 aacatgaact tcttttgttt ctggataaag tccgttttca taaccaagtg tctttagttt
     8341 ctctcccaaa atagctttcg tcgcaatttg tgccattgga attcctgtca ccttacttaa
     8401 gaatggaaca gtccgacttg ctcgaggatt gacttcgatc acataaacct cattttcatg
     8461 gatgacaaac tggatattca tcatcccgac acaattcaaa cctaatgcta agcggatcgt
     8521 gtaatcttcg atcgttttct tgatcgattc agataatgtt tgaggtggat acacagccat
     8581 tgaatcccct gaatggacac cagcacgctc aatatgctcc atgatccctg ggatgagtac
     8641 tgtttcaccg tcacaaatcg catccacttc gcattcacca ccaagcaagt aacggtccac
     8701 tagtactgga tgttctggag atgctttcac agcatgttcc atgtaatctt ctaaatctcg
     8761 ctggttttca acgatttcca tcgcacgtcc gcctaataca tagcttggac gaacaagaac
     8821 tggataacca attctcgaag cgataaccac ggcttcttct ttacttgtag cagtatcccc
     8881 taaaggttga ggaatatcca actctttcag cgcttgctcg aataaatcac gattttccgc
     8941 acgatccaag tcttcgatcg ttgtccctaa gatttttaca ccagcagcag tcaaaggttc
     9001 ggctaagttg attgctgtct gaccaccaaa ttgaacgatt actcctatcg gctgttctag
     9061 ctcgatcaca ttcatcacat cttcgaaggt caatggctca aaatataatt tatccgaaat
     9121 agaaaagtca gtcgaaactg tttcaggatt gctgttcatg atgattgctt cgtatcctgc
     9181 ctgctggatt gctttgacag aatgcacagt cgcatagtca aattcaaccc cttgtccgat
     9241 acggatagga ccagatccca gtactagaat tgagggcttt tctgatcgtg aactttcatt
     9301 ttcatattca taggaactat aaaaataagg tgtttttgat tcgaattccg ctgcacaagt
     9361 gtcaaccatc ttatatacag gaatcaaatc attttctttt ctcatctggc gaacggcttc
     9421 ttcgtttatt tcccacagct ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt
     9481 tttcaatatc ttgtcatcca acggctgctg tttcaactgc tgttcgattt ctacgatatg
     9541 caacagttta tctaaaaaga ataaatcgat ttttgtcaaa tccgctaatt cttctatggt
     9601 gtaccctctt ctaatagctt cagctacata aaacaggcga tcatcttgtg ctcgaaccac
     9661 tttttggatt aggtcatcgt ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt
     9721 accgatttct aaagaacgca cagctttcaa taaagactct tcgatattcc gaccgatagc
     9781 catcacttca ccagttgctt tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt
     9841 atcaaacggc cagcgaggaa ttttagctac aacataatct aatgcaggtt caaattctgc
     9901 aaaagtcgtt cctgtaacag gatttttcat ttcgtctaat gttaatccaa gcgctatttt
     9961 agcagctagc ttagcgatcg gatagccagt cgccttactt gctaacgcag atgaacgaga
    10021 aacacgcgga ttgacttcaa tcacataata attaaaacta tgtggatcaa gagcgagttg
    10081 gacattacag cctccctcga tcttaagtgc tcgaataatc gaaagagaag catcacgaag
    10141 catttgatat tcatggtcag acaatgtttg actaggggca aatacgatag aatcacctgt
    10201 atggataccc accgggtcaa aattttccat attgcagaca acgattgcat tatcagccga
    10261 atctctcatc acttcatatt caatttcttt aaaacctgca atacttcgtt caatcaaaca
    10321 ttgggtagcg ggggaaagac ttaatccatt ttctgatacg atacgtaatt cttcttcatt
    10381 ttcacacata cctccaccag ttccgcctaa agtaaaggct ggacgaacga tgacaggata
    10441 gccgatttta tttgcaaaat ctacagcttc ttctactgta ttgacgattt cagactcggg
    10501 gattggctga ttcaattctt tcatcaactg tttgaataga tcacgatctt ctgcttgatc
    10561 gatcgcagaa agctttgttc ctagaagttc tatgttcaat tcttctaaaa tacctgatgc
    10621 tgccagctcc attgccatat tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc
    10681 tggacgctct tttcgaagaa tccttgatac aaattcaagt gtgatcggtt cgatatatac
    10741 ttgatctgcc acttctttat ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac
    10801 ttcatatcct tcttctttta aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc
    10861 ggcttgacca atgatgatcg gaccagatcc aatcaccata attttcttga tatctgtacg
    10921 tttcggcatc tgtttactgc tcctttcctg cgtccatcat ttccataaat tcatcaaata
    10981 aatgtagtgc atcatgcgga ccaggggcag catctggatg aaactgcaca ctgaaagcgg
    11041 gataatctct gtgtttgact ccttctactg ttccgtcatt tacttctaca tgagttacga
    11101 tgagtttttc tggatcaatc gtggcttcat cgactgcata gccatgattt tgagaagtaa
    11161 aatcaattct tccggtcgct acttctcgta ccgggtgatt taatcctcga tgtccgaatt
    11221 tcattttata ggtatctgca ccgttggcta aagcaaataa ctggtgaccg agacaaatac
    11281 caaaaatcgg tacttttcct tggatttttt tgatcatttc gattgcttct ggcacatctt
    11341 ttggatcacc aggtccattc gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct
    11401 cggcatctgt gttataggga agtactgtga ggttacagtt tcttttactc aattctcgta
    11461 aaatactatg tttcaagccg aagtcaatga caatgacttt ccgacctgtt cctggacttg
    11521 gataaggttt gttagttgag acttgctgca cttgattggt tgctagaacg gttgctttca
    11581 gctgatcaaa tgcatgttcg aaggaatctc cagcatcaac tatactagct ttcatcgttc
    11641 cgacttcacg gattttgcga gtaagggcgc gcgtatcgat acctgatatt ccaggaattc
    11701 ctttttgttt caaaaatgaa tctagcgtca tttggtttcg ccagtttgaa gctacacgtg
    11761 catgttcttt tactactact cctttgcatg ttggagaaat cgattcataa tcatctctat
    11821 ttaccccata attacccacc ataggatagg taaatgtaat gatttgaccg ttaaaacttt
    11881 gatcagtgat agtttcttga taaccagtca tacttgttgt aaaaacgatt tctccgaaga
    11941 cactagcatc tgcaccaaaa gcctttcctt cgaaaactgt tccatcttct aatataagca
    12001 aacgttccat acttttattc tcccttctgc cacacaagtt ttccgttgac aaaagtcatt
    12061 tgtgtttcgc cttttacttt ccagccgata aaaggtgtat tctccccttt tgataaaaag
    12121 tcttctttat cgatcgtgca agtttgtgta atatcaaaaa ctgctacatc agcgggggca
    12181 ccaaccgtta gcgttcctgc attcaaacca aaaatctcag ccggtttaac tgccaaccaa
    12241 ttgatcactt gttccaacgt gaatcgacca gtttctacaa aatgagtata gatcagttga
    12301 aacgctgttt cgcttcctac gataccaaat ggtgatttca tgaaactttg gcttttttct
    12361 tctaatccat ggggggcatg gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct
    12421 tcgatcaaag cttctctgtc ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg
    12481 aaatcttcgg gaatatcttc gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt
    12541 acgtggattc ctgctttttt tgcgtctctg attacacgga cactttcttt agtagaaaca
    12601 tggcaaacat ggtaatgaac accagttgct tcagctagca acagatctct agcaatttga
    12661 gaagactcgg ttacgcttaa gatcccggga agaccaagtt cttctgcctt cttgccagcg
    12721 tgcataacgc cgccaaataa taaagattca tcttctgtat gggctactag ggctttattg
    12781 ttttttgcag cttctttcat agccagatac atcgtcccag ctgtttggac acctacaccg
    12841 tcatttgtga aagcaaaagc accttcttca atcagtgctt cttgatcaac taatttctca
    12901 cttcgcagat tttctgtgat tggcgcatac tgcaatacct tcacaactgc atcttttcta
    12961 atgatgtcat atacttgtct cagcttttct gccgtatcag gtacaggatt taaattcggc
    13021 atcgcgcata cagtagtgaa accgccacga gcagctgagc gagtcccagc ttcgatcgtc
    13081 tccttgtatg taaatcctgg ctctctcaaa tgaacatgga cgtccactaa accaggtgtg
    13141 atcaattgtc cctttgcatc aaacacttca tcaaattcgg cttctgagaa tcctgtaccg
    13201 attgctttga ttcgcccatc ttctatccaa atttctgctg gtgtcgtcat attttttcgt
    13261 gtattgatct gaccgttttt aatgagtgtt ttcatttttt catcctccta taattacgct
    13321 tttccattta gcactgcttc taagattgcc attctgacga atactccatt gctcatttgc
    13381 tgaacgatcc gcgactgtaa tccttccact aatgaatcag ctaattctac atcacggtta
    13441 actggtgctg ggtgcataat gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt
    13501 aacccatgtt cctgatggta gccttctttt gagaaacttt cttttccatc atggcgttcg
    13561 tgttgcaccc gaagcatcat catgacatca acttgatcta gtagttcatc caatggcata
    13621 taatgtccgt acacttcgaa ttcatcgtcg taccattctc taggacctga gaagaatact
    13681 tgtgcgccta gacgttttaa catctgcata ttggatttag ctacgcgaga gtgagtgata
    13741 tcacctacga tggcaacttt cagaccttca aaatgaccaa attcttcata aatggtcatc
    13801 aaatcaagca agcattgggt tggatgctgt ccgcttccgt ctcctccatt gataatcgaa
    13861 cactggattg ttttactttg tatcagttcg tcataatagt tctcatcacc atgacggata
    13921 accgctacgt ctacgcctaa tgctgacatc gtcagtactg tatcgtatag tgtttcccct
    13981 ttttggacag agcttgtgct tgcttcaaat tcaatcactt ctagccctag ttttttctcc
    14041 gctacatcga agcttttatg tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag
    14101 tactggcgct tttgcggagt ccaattagca ccttgcttga attcctgccc tctgcggatc
    14161 aagcccataa cttcttgatc agtaagtgcc tcaacggtca ataaatgctt taagctgata
    14221 cgttctgatt tgatgatcat aatgtttcct ccttagtttt cttcgcttcg tgaagtttct
    14281 ggtaagataa gatttaaaat gatgccaagt acagttgcta atgccattgc cgacaatgta
    14341 aatgtgccta cttcaaagac gagtccgcca attcctacta ctaagataac tgaagcaatc
    14401 aacagatttt tctttttatc aaaatcgatt ttgttctcga ttaaaatctt cattccactt
    14461 gcggcaatta ctccaaataa gataaaactg attcctgaga tgactggtcc agggatactt
    14521 aaaatcaaag cgcttaattt tcctacgaat cctaaaatga ctgcaaatac tgcagctcca
    14581 ccgatcacaa acacgctatg gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa
    14641 cttgttacag gaggacctcc gacaaaggct gccacgattt gtgctaatcc atcacctgtc
    14701 aacgtcttat gaagacccgg ttcttcaaag aaattacgtt tagttagttt attcaatacc
    14761 ataagatggc cgatatgctc tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt
    14821 gctccaagat atagctttgg ttcatattgt acaaagggaa tctcaaagtt aggaacagca
    14881 aaccaggcag cgtctacgat tggcttagta tccacgattc caaacagcaa ggctacgatg
    14941 tatccgctaa taatacctag taaaatcgga atcaatccga gaaaacctct tagccacatg
    15001 ttgaagaaaa ttgttaatcc taatgtgatc agcgctacag caatatactt gaaatcatac
    15061 gtgccctgat tatacatagc atttgttgct gcatttgccg caagtcccaa cccgataacc
    15121 atgatgactg gaccgacgac gattggaggc aagattttat ccagccatgc tgaaccgatc
    15181 tttttgatga tcagcgcaac aatcaaatag actaatccag ttgtgactgc tccttgtgca
    15241 atcgctggat aaccatctgt tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg
    15301 cttcctaagt acgcaggtat ctttccttta gtagtaaata agtacaccaa tgtacctagt
    15361 cctgaactga ctagcgcaat acctggatcg atacctacta agatggggac taacacggtt
    15421 gctccgaaca tcgtgaacaa gtgttgtaaa cttaatccta cccaatgtcc tggggttggg
    15481 cgatcgtgga tatctaacac cacgtcatca ttatgaaatc cttttttctc cgacatgttt
    15541 ctctctcctc taatcttcct ctttcaaaat catgatgcga tcttgtccat caagctcttg
    15601 catctctact aaaatctctt ccgcccgaga tgttggaatg ttcttcccaa cataatctgc
    15661 acggattggc aattctcgat gcccacgatc gaccaggact gctaatgaaa tttttcttgg
    15721 tctgccaaaa tccattactg cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac
    15781 atcatcaatc aaaataactt cttttccttc taaggaaact gggatgtcag aagaatgaag
    15841 ttccggttct tctggatttt cttttttgtc atctcggtat aaagtgatat ccagttctcc
    15901 tacaggaata tcgatatctt ctagctgctt caggcgttcg gcaattcttg atgcaatgta
    15961 gattcctcgt gttttgatac ctaccaatac gatatcttgg atactatggt ttctctcgat
    16021 aatctcataa gtaattcgag tcaatgctcg cttcatggtt acctgatcta ctacttcttt
    16081 tgcttgcatt ttcattcccc ctactttttt gcataaaaaa actcctgccc taagaggaca
    16141 ggaggaatgt ttagactgag cgtatactga tacactgata gctatactat gtcaatgagc
    16201 aatctactta tggcgcacca tacgtatcct tgctcccacc cttcttagcc tcacgggact
    16261 aaacttaaag gaattctatt aagttttatg ctttcgttgt taattactat accgttatct
    16321 aaattaaaat gcaatgtttt ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag
    16381 aggtgcttca aagatcatct tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc
    16441 atggagaaac tgaccattgc ctttcaaagt tttcttcggt ccgtatactg gatctccagc
    16501 taacggataa ccgatatact tcatatgcac acggatctgg tgtgttcttc cggtttctag
    16561 ttttaattca accagagtaa atccttcgaa acgttccaat acagtaaaat gtgtgacagc
    16621 cgacttccca tcttctctta tcgcctgcat tttccggttt tccttcgaac ggccgattgg
    16681 cgcattgatg gttccttttt cgtgaggaat ctctccatgg actaaagcaa catatttgcg
    16741 caaagaggtc ttatctttta attgctgagc taatgattcg tgtgcctggt catttttggc
    16801 aaccattagc agtccggaag tatctttgtc gattcggtga acaatacctg gacgaataac
    16861 gccgttgatc gtagaaagat ccttgatatg gtacatcagc gcattgacca ttgtgccatg
    16921 cgtatgacct gctgaaggat gaacgaccat tccttgaggt ttgtttatca ccgctacatc
    16981 ttcatcttcg taaacgatcg tcaacgggat attttctggg accacatcta atgtttctgg
    17041 ttcggggatc aagacagtaa tctcgtctcc ttgttttact ttgtaatttg ctttgactgg
    17101 ctgtccattg acagaaaccg ctccattttt cagccacagc tgaatttgcg aacgactgta
    17161 atcggtgaat aaattagtca atactttatc gatcctacca gattcatggt gaatcgtcgt
    17221 tttaagttct gtcatttttt tctttgatcc ttttcatcta aaataatata aattaacata
    17281 caaatcactc cacatacaag tgtcacatct gctacgttaa aaatcggaaa gctcataaaa
    17341 tctgtctgga acatatctac tacatagcca agacgaatac ggtcgataaa attgccaatc
    17401 gcaccagcta gaatcaaact caatccaatc atgaaccagc gattaccttt ttcacgattt
    17461 ttaatcatca atgtgagaat cacaacgact gcaatgagtg tcactatagt aaagaaccac
    17521 atcttccctt ctagcatact ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa
    17581 atacctggta taagattttt cgtctctcca agcgctagat tatcagtgat ccaccatttg
    17641 accaactgat ccactcctac tagcaaagcg ctgaatatcc aataaataat taacaaaaaa
    17701 tcaatccttt tctatttgtt tataaatatc acgaggtcgt acgatcccga gcagattacc
    17761 atcgggattc ccatctttag tgataagtac ggcttccaaa gtcccttgct cacgaaacat
    17821 cgcttcaacc tcgtaaatat gtgttttagc tggaacaaaa tgataatttg tgtactttcc
    17881 gttttggatg atcaaatcca acgctcgttt atttgttaaa tcaaccggac ctttttggct
    17941 ttcttttgct agccaaaacc cgatattacg taaagtaatc aatgctacaa attttccttt
    18001 attgtataac ggaaattgtg aatacctttt ttctgctact atctttaaca gttcaagtaa
    18061 cggaagatct tgttcaaaac ctgttacacg ttttgcaaat cgaggcagca cggtttcagg
    18121 ctgaatcagt tcttgttcga tcagctggat tcgcttgact gtccaatcat ttggctctgc
    18181 aatgacaaaa tcgactgcga tccgatcatg gacgatcgca ttccttaact gagcaagctg
    18241 taaaagatca tcttcgtatt ttctgatcat taattgtttt ctttgagcta ggcgtctgac
    18301 aagttcggta aatcccatat ttcgtgcatt tcccatctcg tctctcatcc atttttcgat
    18361 tcgattgaaa ctgattaaaa aaagatcaga gtttgccata attttcctcc ttgatcaaat
    18421 atgcccggtt ttttatatag aaaaagaacg caatcaacac aataactgca aaaaaactga
    18481 aaaataaatt ttgtaggaaa acggccccca acccagttgc tagaaataaa aacggataga
    18541 tttgaagaat caagtaattc attgtgatca cataagcagg ctttgcttct ttcactgtat
    18601 aaacggggct ttttagccaa taaaaggtga cctgcatcgt aaagggcact tccagatcaa
    18661 gcgttatctt ttgttcatga ctgagaacac tgcgtttttc attgttgatt cttagctgaa
    18721 tcgagctacc cataccgtaa aaaccggttt ttcttataaa tgtcgttttc atcataccct
    18781 cctattatgc tttagtatag ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa
    18841 caaaattaat tccctatcgt ctaaaaaaag aagctgtagt caaaatacag ctccttcgat
    18901 ggtagattat aatcaagctt aaaacaatcc caacacttct ccgtcatttg tcacgtccat
    18961 attcaatgca gcaggttttt taggcaaacc aggcatcgtc ataacatctc cagtcaaggc
    19021 tactaaaaat cctgccccta attttgggat gatttcgcgg atcgtgatgg tgaaatcttc
    19081 cggtgcacct agcagtgaag gatcatcaga aaatgaatac tgtgtttttg ccatacaaat
    19141 cggcagtttg tcccatccat tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct
    19201 gaaaacaaca ccatttcctc cgtaaatttt cttgacgatt gtttctgtct tttcttgtat
    19261 cgtttcttct aggcggtata atggcttgta attagcttct tttctatcaa tcaatcgtac
    19321 tacagctttt gctaaatcaa cgccgccttc tgctccatct gcccatacac tcgctctctt
    19381 ggcaagaatc ccctgatttt cacacaagtg ctccaacagt gtgagttcat tgtctgtatc
    19441 tgttacgaat tcattgattg cgacgatcac aggtagctga tattgttcca tattacgtat
    19501 atggcgcttt aaatttgcaa aacctacttt caaagcatca agattctcat ttttaagttc
    19561 atcttttttc attccgccat gcattttcaa tgcacggaca gtggcgacaa tcaccacagc
    19621 atccggcgct tttttcaagt taggtacttt gatatctaaa aatttctctc cgccaagatc
    19681 tgcaccaaat cctgcctctg tgacaacgta atctgctaat ctcaatgctg tcttggtagc
    19741 taaaatacta ttgcagccat gagcgatatt ggcaaacggt cccccgtgga caaaagctgg
    19801 tgttccgtaa attgtttgta caagattcgg cttgattgca tctttcagca gtaatgccag
    19861 agcgccttct gctttcaagt ctccagcagt cacaggctga cggtcaaatg tatagccgac
    19921 aacgatcctt gcaagacgag cctttaaatc ttcaaggtct gccgcaagac acaagatagc
    19981 catgatttcg ctggcaactg tgatatcaaa cccatcttct cttggaacac cttggattgg
    20041 cccacccaag ccaacaatta ctttacgcag ttcacgatca ttgagatcga ctacccgttt
    20101 ccaaatcact cggcgggcat cgatgttcaa ttcatttcct tgatgaatat gattatctaa
    20161 aagagcagat aatgcattat tagctgttgt gatagcatgc atgtcaccag taaaatgcaa
    20221 gttgatatct tccataggca atacctgtgc atatcctccg cctgcagcac cacctttgat
    20281 tcccatcaca ggaccaagtg agggttctcg caatgcaata acagattttt tattgatttt
    20341 attgagcgcg tctcctaacc ctacagtgat cgttgacttt ccttcgccag ctggtgttgg
    20401 attgatggaa gtaactaaga tcaaatgccc ctcagcgtta tcctttgatc gattgattcc
    20461 agaaaaattg attttcgcct tatatttccc atacagttcc aaatcgtctt ccttaagatc
    20521 taatttacta gccacttcaa caatcggttc tagctctgtt tcttgtgaaa tttgcagatc
    20581 attcttcatt tgattacccc ttttcacgaa ctttattcaa caaaatatct tctttgttaa
    20641 gatatgccaa ttataccttt ttttattcag aaaaaaaaca taaaattaca ggtttttaat
    20701 ttatttgacg tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg
    20761 tcgtgagtgc tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct
    20821 ttatcagtta tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg
    20881 gattcaaaac tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag
    20941 gaacacaagc tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg
    21001 aatagataaa caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc
    21061 ttttcctgta aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt
    21121 tcgtataagg ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa
    21181 ggactacttg atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca
    21241 tcatttgcac cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc
    21301 ggtctttcca aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac
    21361 gccgcaaacc tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat
    21421 aaggacctgt gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg
    21481 accaatagat gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct
    21541 aacctctcac cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg
    21601 agaatctctt tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc
    21661 tagtggaggt ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct
    21721 ttctataggg acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct
    21781 aaagaaaaac gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt
    21841 ttctacggtc gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat
    21901 ccaagaactt ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa
    21961 taaaggagca gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac
    22021 tctttaaatt aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa
    22081 ttgtgatcaa acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt
    22141 aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat
    22201 tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt
    22261 tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc
    22321 attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc
    22381 agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact
    22441 gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata
    22501 tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt
    22561 cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat
    22621 gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc gattgctgct
    22681 gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc
    22741 aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc
    22801 aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga
    22861 tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc
    22921 gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct
    22981 gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat
    23041 agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc
    23101 ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt
    23161 tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt
    23221 accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct
    23281 cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc
    23341 tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc
    23401 acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag
    23461 agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc
    23521 taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt
    23581 tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag
    23641 tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt
    23701 tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc
    23761 atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact
    23821 aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc
    23881 taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc
    23941 cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg
    24001 aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt
    24061 ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa
    24121 cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac
    24181 cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta
    24241 ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt
    24301 tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat
    24361 ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca
    24421 cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat
    24481 caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc
    24541 ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa
    24601 caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt
    24661 tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt
    24721 tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc
    24781 agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg
    24841 cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc
    24901 agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa
    24961 agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat
    25021 gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga
    25081 tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg
    25141 gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa
    25201 caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac
    25261 ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt
    25321 cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg
    25381 tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca
    25441 ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt
    25501 caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta
    25561 ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt
    25621 tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt
    25681 cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta
    25741 cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg
    25801 cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga
    25861 cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc
    25921 gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta
    25981 cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt
    26041 tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt
    26101 ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta
    26161 agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt
    26221 ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg
    26281 ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc
    26341 caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa
    26401 gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga
    26461 tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat
    26521 ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat
    26581 ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa
    26641 caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt
    26701 caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac
    26761 ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca
    26821 gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt
    26881 aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt
    26941 gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa
    27001 tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa
    27061 tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg
    27121 aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc
    27181 ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca
    27241 agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct
    27301 atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg
    27361 cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca
    27421 gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt
    27481 agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc
    27541 ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga
    27601 gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt
    27661 tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc
    27721 tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt
    27781 cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct
    27841 attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac
    27901 gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc
    27961 cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc
    28021 ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc
    28081 taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt
    28141 tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa
    28201 gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg
    28261 atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat
    28321 cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg
    28381 attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac
    28441 taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc
    28501 catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag
    28561 cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg
    28621 ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac
    28681 acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc
    28741 tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt
    28801 ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat
    28861 gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt
    28921 gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt
    28981 attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa
    29041 agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac
    29101 agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag
    29161 caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc
    29221 actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa
    29281 ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa
    29341 agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt
    29401 ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca
    29461 ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac
    29521 caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa
    29581 tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat
    29641 aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa
    29701 atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg
    29761 cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag
    29821 ccggaaataa tatcttaaat aaatgccaaa cggtatgagt aataaaacat taaaagctgg
    29881 ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa
    29941 taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc
    30001 tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca
    30061 caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata
    30121 ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac
    30181 aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt
    30241 gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc
    30301 tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt
    30361 actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa
    30421 gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc
    30481 tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac
    30541 atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt
    30601 ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca
    30661 aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc
    30721 atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc
    30781 acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat
    30841 tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca
    30901 atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag
    30961 tccctccaat gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa
    31021 acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa
    31081 tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc
    31141 caatagcgat gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa
    31201 gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac
    31261 gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg
    31321 tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc
    31381 tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat
    31441 aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct
    31501 ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg
    31561 ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa
    31621 acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag
    31681 gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa
    31741 aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc attgactggg
    31801 aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg
    31861 ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa
    31921 tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc
    31981 ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac
    32041 tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca
    32101 ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt
    32161 ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat
    32221 accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt
    32281 ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta
    32341 tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga
    32401 ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat
    32461 tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc
    32521 atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc
    32581 atctggcata tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc
    32641 ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa
    32701 ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac
    32761 ataataataa tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg
    32821 acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat
    32881 tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaatg cattgaccag
    32941 cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag
    33001 agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc
    33061 gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg
    33121 tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc
    33181 tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga
    33241 tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt
    33301 taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga
    33361 taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg
    33421 atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt
    33481 tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta
    33541 ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa
    33601 ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa
    33661 gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt
    33721 ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct
    33781 atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg
    33841 acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc
    33901 agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg
    33961 gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc
    34021 tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg
    34081 gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct
    34141 tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg
    34201 attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc
    34261 agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca
    34321 atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga
    34381 ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg
    34441 atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt
    34501 agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa
    34561 catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact
    34621 gattatgaga attatttaac cacatttttt atttacagta tctttttact ttcaaatgac
    34681 ttatatagta tgaacaatta ccttttagat aatgatctat tttactctac ctgggcagat
    34741 aaatacaaca caaccattga tagaagccct ttcgttaaaa tcaattgtat tctgaatatt
    34801 taatttattt attgcataga ccaataaaaa tacataaaat ttccatcttc gtttatcagc
    34861 aatttttaat tttactctct gaatatttct taaaagtttt acttgttgaa ttcgaaaaaa
    34921 ggactgtgac aaaagtcttg tcacagtcct attcttccga a
//
LOCUS       DAYYBG010000017        34688 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000017 DAYYBG010000000
VERSION     DAYYBG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.017"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHAUN_000887"
     CDS             591..1280
                     /locus_tag="ACHAUN_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8459610.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHAUN_000888"
     CDS             1267..2475
                     /locus_tag="ACHAUN_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8459611.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHAUN_000889"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHAUN_000889"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8459612.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHAUN_000890"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHAUN_000890"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8459613.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHAUN_000891"
     CDS             complement(6578..7273)
                     /locus_tag="ACHAUN_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8459614.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHAUN_000892"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHAUN_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8459615.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHAUN_000893"
     CDS             complement(8472..8663)
                     /locus_tag="ACHAUN_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8459616.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHAUN_000894"
     CDS             complement(8684..9574)
                     /locus_tag="ACHAUN_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8459617.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHAUN_000895"
     CDS             complement(9561..10328)
                     /locus_tag="ACHAUN_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8459618.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHAUN_000896"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHAUN_000896"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8459619.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHAUN_000897"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHAUN_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8459620.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHAUN_000898"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHAUN_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8459621.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHAUN_000899"
     CDS             14899..15282
                     /locus_tag="ACHAUN_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459622.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHAUN_000900"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHAUN_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8459623.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHAUN_000901"
     CDS             complement(16226..17044)
                     /locus_tag="ACHAUN_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8459624.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHAUN_000902"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHAUN_000902"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8459625.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHAUN_000903"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHAUN_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8459626.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18173..19507
                     /gene="dnaA"
                     /locus_tag="ACHAUN_000904"
     CDS             18173..19507
                     /gene="dnaA"
                     /locus_tag="ACHAUN_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8459627.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19705..20835
                     /gene="dnaN"
                     /locus_tag="ACHAUN_000905"
     CDS             19705..20835
                     /gene="dnaN"
                     /locus_tag="ACHAUN_000905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8459628.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21064..21306
                     /gene="yaaA"
                     /locus_tag="ACHAUN_000906"
     CDS             21064..21306
                     /gene="yaaA"
                     /locus_tag="ACHAUN_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8459629.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21293..22417
                     /gene="recF"
                     /locus_tag="ACHAUN_000907"
     CDS             21293..22417
                     /gene="recF"
                     /locus_tag="ACHAUN_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8459630.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22414..24360
                     /gene="gyrB"
                     /locus_tag="ACHAUN_000908"
     CDS             22414..24360
                     /gene="gyrB"
                     /locus_tag="ACHAUN_000908"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8459631.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24380..26851
                     /gene="gyrA"
                     /locus_tag="ACHAUN_000909"
     CDS             24380..26851
                     /gene="gyrA"
                     /locus_tag="ACHAUN_000909"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8459632.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27087..27386
                     /gene="rpsF"
                     /locus_tag="ACHAUN_000910"
     CDS             27087..27386
                     /gene="rpsF"
                     /locus_tag="ACHAUN_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8459633.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27436..27948
                     /gene="ssb"
                     /locus_tag="ACHAUN_000911"
     CDS             27436..27948
                     /gene="ssb"
                     /locus_tag="ACHAUN_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8459634.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27974..28210
                     /gene="rpsR"
                     /locus_tag="ACHAUN_000912"
     CDS             27974..28210
                     /gene="rpsR"
                     /locus_tag="ACHAUN_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8459635.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28353..30326
                     /locus_tag="ACHAUN_000913"
     CDS             28353..30326
                     /locus_tag="ACHAUN_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8459636.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30332..30784
                     /gene="rplI"
                     /locus_tag="ACHAUN_000914"
     CDS             30332..30784
                     /gene="rplI"
                     /locus_tag="ACHAUN_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8459637.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31058..32425
                     /gene="dnaB"
                     /locus_tag="ACHAUN_000915"
     CDS             31058..32425
                     /gene="dnaB"
                     /locus_tag="ACHAUN_000915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8459638.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33205..34497
                     /locus_tag="ACHAUN_000916"
     CDS             33205..34497
                     /locus_tag="ACHAUN_000916"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8459639.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10563 a         6737 c         6861 g        10527 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgcggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gagtcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa tggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggtgatttc ctgtgtattc cgtctcctaa ctattggtat atctatattt
    17941 tttatccaca gatgcaagag gaaatctatt gatttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    18061 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    18121 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    18181 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18241 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18301 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18361 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18421 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18481 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18541 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18601 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18661 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18721 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18781 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18841 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18901 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgcctcgttt ctcgctttgc
    18961 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    19021 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    19081 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    19141 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    19201 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19261 ttatcacatc caattgaaag atatcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19321 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19381 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19441 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19501 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19561 aaaagttatg cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19621 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19681 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19741 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19801 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19861 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19921 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19981 tttactttag aagttttaga aaataaacaa gtagcgatta cttctggaaa agcgaatttt
    20041 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    20101 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    20161 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    20221 ttattggctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20281 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20341 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20401 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatcctga taccaatcgt
    20461 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20521 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20581 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20641 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20701 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20761 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20821 gtacgtacaa attaacaatg attttcattg ttcttgaaaa tttaattgaa aagaaataga
    20881 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20941 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    21001 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    21061 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    21121 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    21181 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21241 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21301 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21361 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21421 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21481 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21541 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacaaaaaaa
    21601 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21661 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21721 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21781 tttaaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21841 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21901 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21961 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    22021 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    22081 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    22141 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    22201 taaagaagag acgggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22261 tgatagtcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22321 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22381 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22441 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22501 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22561 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22621 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22681 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22741 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22801 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22861 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22921 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22981 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    23041 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    23101 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    23161 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    23221 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23281 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23341 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23401 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23461 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23521 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23581 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23641 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23701 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23761 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23821 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23881 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23941 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    24001 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    24061 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    24121 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    24181 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24241 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24301 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24361 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24421 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24481 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24541 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24601 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24661 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24721 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24781 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24841 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24901 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24961 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    25021 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    25081 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    25141 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    25201 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25261 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25321 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25381 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25441 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25501 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25561 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25621 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25681 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25741 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25801 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25861 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25921 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25981 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    26041 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    26101 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    26161 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    26221 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26281 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26341 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26401 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26461 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26521 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26581 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26641 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26701 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26761 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26821 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26881 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26941 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    27001 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    27061 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    27121 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    27181 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27241 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27301 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27361 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27421 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27481 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27541 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27601 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27661 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27721 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27781 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27841 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27901 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27961 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    28021 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    28081 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    28141 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    28201 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28261 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28321 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28381 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28441 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28501 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28561 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28621 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28681 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28741 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28801 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28861 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28921 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28981 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    29041 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    29101 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    29161 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    29221 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29281 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29341 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29401 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29461 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29521 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29581 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29641 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29701 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29761 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29821 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29881 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29941 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    30001 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    30061 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    30121 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    30181 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30241 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30301 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30361 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30421 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30481 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30541 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30601 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30661 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30721 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30781 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30841 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30901 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30961 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    31021 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    31081 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    31141 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    31201 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31261 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31321 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31381 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31441 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31501 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31561 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31621 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31681 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31741 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31801 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31861 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31921 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31981 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    32041 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    32101 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    32161 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    32221 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32281 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32341 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32401 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32461 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32521 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32581 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32641 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32701 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32761 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32821 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32881 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32941 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    33001 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    33061 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    33121 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    33181 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33241 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33301 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33361 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33421 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33481 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33541 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33601 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33661 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33721 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33781 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33841 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33901 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33961 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    34021 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    34081 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    34141 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    34201 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34261 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34321 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34381 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34441 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34501 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34561 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34621 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt
    34681 tttccgaa
//
LOCUS       DAYYBG010000018        34685 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000018 DAYYBG010000000
VERSION     DAYYBG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.018"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(129..977)
                     /locus_tag="ACHAUN_000917"
     CDS             complement(129..977)
                     /locus_tag="ACHAUN_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8459640.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1201..3141)
                     /gene="thrS"
                     /locus_tag="ACHAUN_000918"
     CDS             complement(1201..3141)
                     /gene="thrS"
                     /locus_tag="ACHAUN_000918"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8459641.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3478..3657)
                     /locus_tag="ACHAUN_000919"
     CDS             complement(3478..3657)
                     /locus_tag="ACHAUN_000919"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8459642.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3704..5527)
                     /locus_tag="ACHAUN_000920"
     CDS             complement(3704..5527)
                     /locus_tag="ACHAUN_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8459643.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5805..8084
                     /gene="helD"
                     /locus_tag="ACHAUN_000921"
     CDS             5805..8084
                     /gene="helD"
                     /locus_tag="ACHAUN_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8459644.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8177..8824)
                     /locus_tag="ACHAUN_000922"
     CDS             complement(8177..8824)
                     /locus_tag="ACHAUN_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459645.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9003..9722)
                     /locus_tag="ACHAUN_000923"
     CDS             complement(9003..9722)
                     /locus_tag="ACHAUN_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8459646.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9853..11004)
                     /locus_tag="ACHAUN_000924"
     CDS             complement(9853..11004)
                     /locus_tag="ACHAUN_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8459647.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11221..11961)
                     /locus_tag="ACHAUN_000925"
     CDS             complement(11221..11961)
                     /locus_tag="ACHAUN_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8459648.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11965..12288)
                     /gene="rsfS"
                     /locus_tag="ACHAUN_000926"
     CDS             complement(11965..12288)
                     /gene="rsfS"
                     /locus_tag="ACHAUN_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8459649.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12345..12938)
                     /gene="yqeK"
                     /locus_tag="ACHAUN_000927"
     CDS             complement(12345..12938)
                     /gene="yqeK"
                     /locus_tag="ACHAUN_000927"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8459650.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12922..13572)
                     /locus_tag="ACHAUN_000928"
     CDS             complement(12922..13572)
                     /locus_tag="ACHAUN_000928"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8459651.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13582..13893)
                     /gene="yhbY"
                     /locus_tag="ACHAUN_000929"
     CDS             complement(13582..13893)
                     /gene="yhbY"
                     /locus_tag="ACHAUN_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8459652.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13909..15018)
                     /gene="yqeH"
                     /locus_tag="ACHAUN_000930"
     CDS             complement(13909..15018)
                     /gene="yqeH"
                     /locus_tag="ACHAUN_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8459653.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15015..15542)
                     /locus_tag="ACHAUN_000931"
     CDS             complement(15015..15542)
                     /locus_tag="ACHAUN_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8459654.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15722..16507)
                     /locus_tag="ACHAUN_000932"
     CDS             complement(15722..16507)
                     /locus_tag="ACHAUN_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8459655.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16500..17369)
                     /gene="accD"
                     /locus_tag="ACHAUN_000933"
     CDS             complement(16500..17369)
                     /gene="accD"
                     /locus_tag="ACHAUN_000933"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8459656.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17370..18749)
                     /locus_tag="ACHAUN_000934"
     CDS             complement(17370..18749)
                     /locus_tag="ACHAUN_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8459657.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18757..19176)
                     /gene="fabZ"
                     /locus_tag="ACHAUN_000935"
     CDS             complement(18757..19176)
                     /gene="fabZ"
                     /locus_tag="ACHAUN_000935"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8459658.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19201..19677)
                     /gene="accB"
                     /locus_tag="ACHAUN_000936"
     CDS             complement(19201..19677)
                     /gene="accB"
                     /locus_tag="ACHAUN_000936"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8459659.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19680..20915)
                     /gene="fabF"
                     /locus_tag="ACHAUN_000937"
     CDS             complement(19680..20915)
                     /gene="fabF"
                     /locus_tag="ACHAUN_000937"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8459660.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20934..21671)
                     /gene="fabG"
                     /locus_tag="ACHAUN_000938"
     CDS             complement(20934..21671)
                     /gene="fabG"
                     /locus_tag="ACHAUN_000938"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8459661.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21683..22600)
                     /gene="fabD"
                     /locus_tag="ACHAUN_000939"
     CDS             complement(21683..22600)
                     /gene="fabD"
                     /locus_tag="ACHAUN_000939"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8459662.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22707..22931)
                     /locus_tag="ACHAUN_000940"
     CDS             complement(22707..22931)
                     /locus_tag="ACHAUN_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8459663.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22984..23946)
                     /locus_tag="ACHAUN_000941"
     CDS             complement(22984..23946)
                     /locus_tag="ACHAUN_000941"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8459664.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23956..24396)
                     /locus_tag="ACHAUN_000942"
     CDS             complement(23956..24396)
                     /locus_tag="ACHAUN_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8459665.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24702..25667
                     /locus_tag="ACHAUN_000943"
     CDS             24702..25667
                     /locus_tag="ACHAUN_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8459666.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25724..25954)
                     /locus_tag="ACHAUN_000944"
     CDS             complement(25724..25954)
                     /locus_tag="ACHAUN_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8459667.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26040..26927)
                     /locus_tag="ACHAUN_000945"
     CDS             complement(26040..26927)
                     /locus_tag="ACHAUN_000945"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8459668.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27016..28239)
                     /locus_tag="ACHAUN_000946"
     CDS             complement(27016..28239)
                     /locus_tag="ACHAUN_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8459669.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28256..29302)
                     /locus_tag="ACHAUN_000947"
     CDS             complement(28256..29302)
                     /locus_tag="ACHAUN_000947"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8459670.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29466..31193)
                     /gene="ptsP"
                     /locus_tag="ACHAUN_000948"
     CDS             complement(29466..31193)
                     /gene="ptsP"
                     /locus_tag="ACHAUN_000948"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8459671.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31193..31459)
                     /locus_tag="ACHAUN_000949"
     CDS             complement(31193..31459)
                     /locus_tag="ACHAUN_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8459672.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31938..34172
                     /locus_tag="ACHAUN_000950"
     CDS             31938..34172
                     /locus_tag="ACHAUN_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8459673.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34320..34640
                     /locus_tag="ACHAUN_000951"
     CDS             34320..34640
                     /locus_tag="ACHAUN_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8459674.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9803 a         7493 c         6051 g        11338 t
ORIGIN      
        1 cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa acactttgat
       61 ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata taaaaaatag
      121 taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt ttttttcact
      181 ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc gatcgtagca
      241 aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt tggtctagat
      301 gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg cgtcaacgca
      361 atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc tgcacttcta
      421 cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag ttgcgggagc
      481 aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca tttttgcaga
      541 atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt ctctgccgat
      601 tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac tgtttctttt
      661 atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga ctcatttgct
      721 tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat cgtgattttg
      781 aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt cggcgcactc
      841 aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc aatattaccg
      901 ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat actttcaata
      961 cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt aagtatacca
     1021 tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac
     1081 agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct ttgaggaata
     1141 cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga aatttaccta
     1201 ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa agatattcaa
     1261 atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg catcttccaa
     1321 ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg cacgtatctt
     1381 atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca tttgcaaacg
     1441 ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga tgattgttgc
     1501 ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta aataagcgac
     1561 aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat gcgtattttc
     1621 cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt ctaattgtat
     1681 agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta gttttggtcc
     1741 gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat ccatagcggc
     1801 tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt tatctgtatt
     1861 gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt cagcatatac
     1921 tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag gacgaacaaa
     1981 tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca aagcacctga
     2041 tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt cacgataaga
     2101 atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc gaagaacaag
     2161 catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat gatcccagtg
     2221 tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat gttggtaacc
     2281 tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg ttgccccttt
     2341 tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca agtcaagttc
     2401 ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga tatattcttt
     2461 caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt gattgtttga
     2521 gtttccacgc caataagcac ctgccactga taataattta aatacttgta tacggccagt
     2581 ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat aagcagtaat
     2641 cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac ttgcaaaaat
     2701 ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt ctttgacgat
     2761 tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt tttgtccatt
     2821 atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt ggatatttgg
     2881 atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga ctaagccaag
     2941 tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga ttggacgagt
     3001 caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta agctttttga
     3061 aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga ctgcaccatc
     3121 tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt gatcagatca
     3181 tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag gacgaaaagt
     3241 tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta acggtccgac
     3301 cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt
     3361 ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct ttctcatcaa
     3421 tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa actaaactta
     3481 ttttttccat tccccattta caccgtatgt tccttttttc atctcattga tgatgacatg
     3541 gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat ctttcatcat
     3601 attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga atggcataat
     3661 gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact atgtttgtct
     3721 tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca ctcaaaatag
     3781 agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt agtcccgtac
     3841 ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac tgtttcgcta
     3901 ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga tcatctttca
     3961 agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc attgcaatac
     4021 cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc atcgcaacag
     4081 gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc aagctacggg
     4141 catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca gatccatccc
     4201 cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt gtctttgctg
     4261 cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga tcaattgtta
     4321 catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag gcacgtccaa
     4381 tctttacttc atgattattg actactgctt ggattccttg accggtcact tctttcagtt
     4441 gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga gctaagatat
     4501 ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt tttattccat
     4561 tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct gttttatcaa
     4621 aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt acaacgatcc
     4681 cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct agaatcagtg
     4741 gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt gagaaccacc
     4801 aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa cgatcagcta
     4861 aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata atcatttgat
     4921 actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct ccatttaaag
     4981 accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca cctgtcaaag
     5041 aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt acaagttcgc
     5101 caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata tcctgcaatt
     5161 ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac tgcagttctt
     5221 tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc atgatcaata
     5281 tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta
     5341 atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa aacatcaaga
     5401 aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa
     5461 caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat tttttgaaat
     5521 gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata aatttccagc
     5581 tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat aggccttaat
     5641 tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa caaaaatatt
     5701 cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga aaaactatgc
     5761 tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac
     5821 tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa aatatgctgg
     5881 atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac acagctaatc
     5941 acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa tatcgccaac
     6001 atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa
     6061 cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa gaagaccgtg
     6121 aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg
     6181 ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt ggtcccgcat
     6241 tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga caatttaaaa
     6301 tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat gatgactttt
     6361 tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc tctacgatac
     6421 aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg attgaaggaa
     6481 tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg ttgtatcata
     6541 atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat ttgttttctg
     6601 attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca cagacattca
     6661 aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag caacaagaaa
     6721 gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga ttgaccttag
     6781 tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg ttccgtgata
     6841 tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg tataaagaaa
     6901 caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag ctcctgaaaa
     6961 aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag
     7021 aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa caaaaggagc
     7081 gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc
     7141 gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac tttctacaaa
     7201 ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg caggaacatt
     7261 tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat
     7321 tctttttact gatgcgccga acatatccag tgacggttac acacaaagct cgctatatct
     7381 ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa gccttgttcc
     7441 caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga aatgaaacga
     7501 tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc caattaacga
     7561 caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt gtgaaagaac
     7621 atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa ggaacaacag
     7681 atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga aaagcccggt
     7741 attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt tatgctcaac
     7801 tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac tttatgaagc
     7861 gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac cgagttttct
     7921 tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc ctttacacca
     7981 tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa agtcctttgt
     8041 taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa agacaactgc
     8101 tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa aaagtaattc
     8161 aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga tattttaatt
     8221 tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct gggtcaaata
     8281 ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga ttccattcaa
     8341 tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc aaaatctctg
     8401 atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg tctatcttct
     8461 catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct aatagtagaa
     8521 agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa
     8581 aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg tccaccatac
     8641 actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga tagccttcaa
     8701 cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc acagctaact
     8761 cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg atttttgctt
     8821 ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga tacttatgct
     8881 ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa gcaagacaat
     8941 ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt aaaactggtt
     9001 gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag atcttccagt
     9061 gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac ctcgttttga
     9121 gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt taatgcttct
     9181 tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc ttcttccatt
     9241 acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc agcgtcttct
     9301 cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc
     9361 atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg attcacattg
     9421 ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc atagcgcaat
     9481 tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc aataatatgt
     9541 tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg gtttgcatgc
     9601 ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt tgcataaatc
     9661 cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca tttacgtccc
     9721 atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc ctgttccatt
     9781 atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt cttctcattt
     9841 ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg gaatattgcc
     9901 tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg cctctttttt
     9961 tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc ttccagcatc
    10021 ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct cttgcttttt
    10081 gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat atctttttgt
    10141 ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg ttttcatgcg
    10201 cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag tagaagataa
    10261 caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac aagttgaaag
    10321 atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt ctaacgcatg
    10381 gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat gatagcctgc
    10441 acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat tttcctttgc
    10501 ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat cgggatacag
    10561 actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag acagttcttg
    10621 aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac tgctgtaatc
    10681 aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg tttgcaagat
    10741 ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga cgggaagctc
    10801 cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat
    10861 agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga cctctgctcc
    10921 tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag gattgtactc
    10981 tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat aaaaaagaaa
    11041 tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat aaactattct
    11101 cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc
    11161 ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca ggcaaaaccg
    11221 ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg cttcgtctgt
    11281 aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc ttaaataact
    11341 ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc gaccagaccc
    11401 ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg
    11461 ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat attctggaaa
    11521 aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat tttcttctaa
    11581 cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca tatagcacag
    11641 cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat cgagcatatt
    11701 gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg caatcatcaa
    11761 catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa aggcaaccgc
    11821 taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt gtaaatgacg
    11881 tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa
    11941 agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga ctagcggagc
    12001 atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga aaacatgtac
    12061 gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt caatacgttt
    12121 tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg cgttgatttg
    12181 acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa catttcgaac
    12241 gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg caatttttaa
    12301 ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt ttttgctacc
    12361 cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg
    12421 gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca tgcaatggct
    12481 cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc tacataaatg
    12541 attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat tgcatgaaga
    12601 atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac aagaccatgc
    12661 cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac gattaccatt
    12721 ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc aatactagct
    12781 ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc tactccaagc
    12841 acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag gttttctcta
    12901 gaataagcag tatattcatt actatagtcc atattcatac aacccttcat tttgaatata
    12961 atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga tcttttgacg
    13021 tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag gatatggagt
    13081 atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag tcagctcatc
    13141 gattttatac cattttggca aatattctac catatcccca ccgataataa agtaataatc
    13201 tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac ttttcccacc
    13261 acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg ctaattccaa
    13321 catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag gtggctgata
    13381 agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt cagccatcac
    13441 taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt gctttttctt
    13501 ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg
    13561 cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc tttgatactt
    13621 ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga cgatttcgca
    13681 atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg tattttgaag
    13741 taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa cgatcattgc
    13801 tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg ctttgctacg
    13861 taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt aaatcaatgc
    13921 tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta ctggcgctgt
    13981 tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca cagaaaactc
    14041 aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct gtaataatcc
    14101 acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat gaatatttac
    14161 atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat caaatcttgc
    14221 aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag gtttgatttc
    14281 ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca tttgatggcg
    14341 atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt
    14401 atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc ccgctgtatt
    14461 tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga cgatatagac
    14521 atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct catgagattt
    14581 cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag cacgttcctt
    14641 catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca ctttgttccc
    14701 aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta gtgaaccatt
    14761 gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta attcattcag
    14821 taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat aatggcgtaa
    14881 tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca aagccgtttg
    14941 cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac agccgataca
    15001 ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg ttttgctaat
    15061 agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt
    15121 gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc acgtatatca
    15181 gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa ttgtttcttt
    15241 gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa aagatcaaat
    15301 ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac aacagggatt
    15361 cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc tggattattc
    15421 catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag attctttaat
    15481 tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta atttgaaaac
    15541 atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc aaccccgtat
    15601 tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca gaaaaagcat
    15661 ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa acaaaaaaca
    15721 actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa
    15781 caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact ttatatcgtg
    15841 gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct aactcgcgta
    15901 gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc
    15961 acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct tccagcatcc
    16021 agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca ccaatgatga
    16081 tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca atagcctctc
    16141 cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg atcaaggtaa
    16201 tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt cgatagcctt
    16261 caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt cctttttgaa
    16321 taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca atcgcttcgt
    16381 catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa
    16441 aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca tgtgctgtct
    16501 tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg gattttctgt
    16561 ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa gaattcagcc
    16621 ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca
    16681 aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc aaaactggca
    16741 gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc ttcattgcta
    16801 tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat tccttcttgc
    16861 atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt tgttgctcgt
    16921 tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat gataaaatta
    16981 ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc agtgaggact
    17041 gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata ttcgggaaat
    17101 gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt ttcatcaata
    17161 atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg agggcatact
    17221 ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca ctttgcccac
    17281 atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg attgatccga
    17341 atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt ttctggtgtc
    17401 caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca
    17461 ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc tgtgataaat
    17521 tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc atggacgatt
    17581 atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta aactgcactg
    17641 tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt ccctggagaa
    17701 ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc gtgtccttgg
    17761 aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc cacctgtttt
    17821 tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg gattcgagta
    17881 ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat cgttccggca
    17941 ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc ttctcttttt
    18001 gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt gcgttgtaaa
    18061 gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa gatctgaact
    18121 tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt tccaaatgcc
    18181 gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt tgattgaact
    18241 ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg ataaccaatt
    18301 ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact tcctggaatg
    18361 actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc cattgcatcg
    18421 atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat ggaggcaaat
    18481 cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt tgtcacgatt
    18541 gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc cgggccaatg
    18601 cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc tgaatatatc
    18661 gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc gatttcaccg
    18721 cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa aggttaattc
    18781 cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc cggctgaagc
    18841 tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa ctttcttacg
    18901 gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt gttcaagaga
    18961 aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc ctggcataac
    19021 aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat ttttcttagc
    19081 aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca ttggataacg
    19141 atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt cctgctttgc
    19201 ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact cgttttctac
    19261 aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca gtttcatcgc
    19321 ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt tgaaagctgg
    19381 tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa cctcgattcc
    19441 atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg cttgttcagg
    19501 tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca tattcatgcc
    19561 tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca aatcgaattc
    19621 cgttagcgag gattgatcaa attgactgac taattctctg atttcattga tatccaaaat
    19681 tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc taatgaatta
    19741 ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc
    19801 tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc taatgccagc
    19861 aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt catcgatttc
    19921 gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat tgcttttgct
    19981 tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc
    20041 ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc agaaccatct
    20101 ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac gatttctcca
    20161 tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag aacgcctgct
    20221 ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc acgattagga
    20281 tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat ctcacaaatc
    20341 gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat gttacggaat
    20401 gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt acaaatacct
    20461 tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc cattggtacg
    20521 aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg gttttcgatt
    20581 gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg attcattttt
    20641 tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc atagatcgaa
    20701 aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg gaaatctttg
    20761 acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat cggtgcgatg
    20821 ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc aataggtgaa
    20881 gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt ttcttatccg
    20941 tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg attttgtgct
    21001 aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat cggaatttgc
    21061 ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt tgtgatgaat
    21121 cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc cacagatttt
    21181 gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat atttccaatc
    21241 aacccagata cactagagag attaatgatg gccccttctc gctgcttcat catttttttc
    21301 aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca ttttttgaaa
    21361 tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt gtttactaac
    21421 acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc tttctcataa
    21481 tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat ttcttctatc
    21541 ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa
    21601 gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac attttttcct
    21661 tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat gtctgcttat
    21721 tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg cttaatactt
    21781 ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta tggatactct
    21841 catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt gtttctttct
    21901 tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag aattgaatct
    21961 gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta tgaaaaggcc
    22021 cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt aaaacagctt
    22081 cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta taatttgcag
    22141 gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg
    22201 cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc atataagcgc
    22261 ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta gctactaatg
    22321 ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca tgctccttca
    22381 ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat
    22441 ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga atctcacttg
    22501 cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc ataccttgat
    22561 attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc aatatatcca
    22621 gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt caggaaatgt
    22681 cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta agtcactaaa
    22741 tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc atcttcaatt
    22801 tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg gatgtcagta
    22861 gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc ttgaattttt
    22921 tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt ataatttaac
    22981 agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc ctgtaaatat
    23041 tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat ccaataagat
    23101 agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga acttttcttg
    23161 cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct gatgcaaaag
    23221 cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa cgtctcgtac
    23281 ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt ctgaatcttc
    23341 tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac ttctggtacc
    23401 gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta agagaatccc
    23461 acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga ttagcttaga
    23521 tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca ttttttcagc
    23581 catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag cgatagcatg
    23641 agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt aatcgggcga
    23701 cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct gttttatcaa
    23761 tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac atcttcgttc
    23821 acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct tggaaagatc
    23881 attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca ttccaaattg
    23941 ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt tcagtgcatc
    24001 cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga ccatttcacg
    24061 atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc ccaactttac
    24121 aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta ctaacttgtt
    24181 aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt ctgaagttgt
    24241 ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg acagatcttt
    24301 gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga agacactaac
    24361 aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac taattagttt
    24421 gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat gcaaccttaa
    24481 aagatcattt ttttactata tgagcttttt ttaagatttt actcatttaa aacaactgat
    24541 aaatcagcat ttataatttc ttttattaga aaaatcatct atctcacata ttttgattat
    24601 caaagtattt tctgtgaaca gaactataga attccctaaa acttgtccat tatttcaaaa
    24661 tttgttatag tctttttatt caaagcgtaa aaggagatcc tatgttaaaa gaaaaaatga
    24721 aaactctccc catgctgccg ggtgtctatc ttatgaaaga ccgagatggt aaggtcatct
    24781 atgttggtaa agcaaaacgg ctcaaaaata gagtaagcag ctattttcat cagaataaac
    24841 agcattcaaa aaaagtatta cgtatgattc atcatataac agattttgat ttcgtcgtag
    24901 tagacacgga attagatgct ttattgctcg agtgtcaatt gatccaacat taccgtcctt
    24961 tttataaccg acaaatgaat tatttcagta attataatta tgtacatatt accaataagg
    25021 gatttgtatt aacagatact cctacggctc gaacatacgg gccttttcgt ctctataaaa
    25081 aaatgccgtc tattttgcgt ataatggaag aaacttatca aatgccttgg ttgtctgaaa
    25141 ttagtttgtt agctttgcga gtccagcttc ctgacctaca ggaaatgact tttgaacaga
    25201 aaaaaaagga actccaaggg ctgtttcaag gccgaaataa aaagctactg acttacttga
    25261 aaaaaagaca acaacatttc atctatcagt tgaatttcga aaaagccggt atgcttcaaa
    25321 aagatatcga attagttact tattttatta gacggatcca ggaacaaaag cagtttttaa
    25381 gaacaccatc gctcaccttc agcatgccat tagcagcaga tgagagtcag aaaaagcact
    25441 acctcgtttg ttatggtcaa cttgcagaaa caatgattac ctctggtgac gtttctcctg
    25501 acttctatta tgaaaagaaa gaagctcatt tatctttgaa acgtcaattg tcaaaagaag
    25561 aaatcgatcc tgtacaaatc ttgatcagtt accgcaaaaa attagaaaaa gaacagtttg
    25621 aaattgaact gttaaacaaa aaagaagctg aaaagcaact aaattagttg cttctcggct
    25681 tcttttaggc atcttcaact tcaaatgtca attatcctaa aatttattgg attaagtcat
    25741 agatttccat cgctacgata tcgatattat caaattcgta ttgctgatga gtatctgttt
    25801 cagttaattc aaatgtatcg gtcgctttat cataagttac tgtacacttc tctacgcctt
    25861 ctttttcaaa acggcgaact tgcagttcat tatctgctga ctcgaccata gcctccagtc
    25921 gttttatgat tgcgactaat tgtgaatgtg tcatcggagg tcctcctttt cttatttctg
    25981 ttatattgta tcaaaagcat agcctacttg cattgatttt ctctaaaaaa actcgttttt
    26041 tatgattttt tttctggtat ttttccttca ggccaccaaa gtttgatcaa agcttgggtt
    26101 ccgtcattct caaaaccttt atctgccaac tcttcatata gtttcaatgc ttgggtagtt
    26161 gctggcaata caagatccat tttttctgct tcttctaatg caatctttaa atctttgata
    26221 aaatgtttga caaaaaaacc aggtgtgtaa tcttctttta aaatacgagg agaataattg
    26281 cttaaagacc agttagctgc acttccacct gaaagcgtct caatcacttt ctttaaatca
    26341 agtccgtttt tttgtgcata gacaagcatt tccgtcattc ctgtcatcgt acctgcaatc
    26401 atgatctgat tagccatttt tgtatgctga cctttcccag cagaaccatg caatgtaaat
    26461 gtcgtaccaa attctttgaa taaaggcagt acttgatcat aaacggcttc ttctccaccg
    26521 accatgatcg tcagtgtacc attctttgct cctaagtcac caccggatac cggtgcatct
    26581 aacgcatctg cgcctttttc ttttgctgtt tttgcaattt tttctgccag gcttggtgta
    26641 cttgtagtca aatcaactaa aatatgtcct gagatatctg cagaaaaaat ccccgactgt
    26701 ccatagtaga tttcttctac atctttgggg taacctacca ttgtaaagag aatattactt
    26761 tgttctgcta ccgcttttgg tgtatctgcc cagatagctc cttctgttac taaaggatca
    26821 gcttttgctt ttgtccgatt atagacagct acttcgtgtc ctgcttctag tagatggcgt
    26881 gcgacagcac ttcccatgac gcctgttccg ataaaaccaa gtttcatttt ttcgacctcc
    26941 taaaaatcaa aaatccttgc ttctatgcta gtatacgcta gaaacaagga ttttttcata
    27001 aaaaatgttt gaattttatc ttttaaatga atttaatttt attttgattc gttcaatcga
    27061 gtcttcttct tcttttcttc gcatctcttc ttcatgataa acaaccatgt cttgataaaa
    27121 ttcgatcata gcttcgccga aatgtgtaga tgaaatatca taaagtttag cttctaaaac
    27181 ttttggatct ttaggaactt cactggaaat ataatcaata agtgctggtg caaagtcttc
    27241 gtcggtttca aaagttttcc ctaatgaagg atgatcgaac aatcggttca aataagcatt
    27301 tccttccacg acacaaggtg ttccggctgc catcgcttct gtataagtaa gcccttgtgt
    27361 ttctgaagta gaagcactga caaaataatc tgccgcttta taataaagtg ctacatcatt
    27421 gttgtccact tctcctgtga actgcacaaa ttcttctaac tgcagttctg agatcaattc
    27481 ctctaactct tctacataag gtccttttcc tacgataact aatctggcgt taggataagc
    27541 ggagatgacc tgaggaaacc cattaatcaa ggcttggata tttttctcat aagaaatcct
    27601 gcttaaagaa agaatcatca aatggttttc ctgaagacct agtgactccc ttaatttgct
    27661 gatatcctgc tgagtgatat cagaacgttt gaatttttca atgtcgatac cagtaggaat
    27721 cacgcgaagc ggtgctatta caccatagct gcgtaatgta tcaataacac gttcacttgg
    27781 acaaactaca cctgtcgtat gatttgcaaa taatctagac aagtatttta catgggattg
    27841 cctgatcact tttcctttac cgatataatg aaggtagtct tcatacatcg tatggtaagt
    27901 atggatcaga ggtattttca acttcttagc taccatcttc ccaagtatcc ctgctccgaa
    27961 ttctgtatgg gtatgaataa gatctagttc tagttctttg gcaaccaaat aagcatacca
    28021 catgcctcta acaacaactc ttcgatcctt gaaagaaata aatggtacac ttggcattcg
    28081 aatcacatct ttttccaact tatccgcatt aggatcggtc gttgtaaaaa tatagacttc
    28141 atgcccatgt ctttctaatt cttgttttaa tgttttgatc gatgtagcaa ctccgctgac
    28201 ttgcggaaag taggtatcgg taaaaaagcc gattttcatg tttttcgcct ccaaactagt
    28261 ccttgacctg atttactttt tttaatttgc tagagacttt ttcattcagt acactttcgt
    28321 atacattttt aagttcttca ccgattttat tgatacttcg ctcttttgct gtttgataac
    28381 cggcttgtgt cgtactcggt agttcttgat tggtaatctg ttggatcagt cggataaatt
    28441 cttcgtttgt cttccccata tagcagttct tgctatcttc taaccagcca ttatacactg
    28501 gaatatctcg aacaagtacg ttttgtttgc ttgctaaagc ttctaaaaca acgatccctt
    28561 ctgtctcttc ataagaaggg aaaaagaata gatctgcacc tgaatacgca ccttctatga
    28621 tctctccacg aatgtaacca ggaaacagga cattttcagg atgatcttct tttacaattt
    28681 tccggatgtt ccgaggaatc gagtacatgg gaacatgacc aaaccaaata aatgtgtatt
    28741 cgggcatctt cttagcaatt tctacgaaat caatgattcc tttacgttcg aaaaacaaac
    28801 cgacacaaat gattactttt tgatccggag acagtttgaa atattctctg aacttttgtt
    28861 ctttcattgg atcagggtga tatttctcta agtcgatccc attggaaata gcttgaatcg
    28921 gcacacggat accatagctt tccaacaact gttttgaata aggtgttggc gtgatcagat
    28981 agtccgcttt ttggtacaat cctaccagat atttttttac gataggagaa agctggttcg
    29041 atccgataaa ggaatttcgg aaatcttcct ctgtagaatg cgcatgatag acaacttttt
    29101 ttccttctct tctggcttta ttgaccatat tatgactatt gattccgtat gtattgatgt
    29161 gcaggatgtc ataatcttct ttttcatcta gtgtataaga gataccgact tccgtcagag
    29221 cacgtttttg atgatcaagc gcacgcccga ttccagattt tgcaagcatc ttttcacttt
    29281 caaaatataa taataccttc accttacgtc ccccttcact tatatagtat aacatagcct
    29341 acaaactgtc actcaaactt aagccctatt tttgtctatt cttactgaca aaaacgtttt
    29401 cttaaaaaag aacaaaaaaa ctggcgtagc tcttttacaa gccacaccag cttttgatac
    29461 gttgtttatt ttacatattc atgaacaagt tcaacgactt cttccatcgt atcgcattct
    29521 ttcaatgcac gatcagcaag ttcagccatt ttcgctgtgt ccaagcgttt cattaaacta
    29581 cgcgttttaa gaatagatgt cgcactcatt gagaactcat ctagacccat acctactagc
    29641 aatggaacag ctgtttgatc tccagccatt tctccacaca tacctgccca ttttccttca
    29701 gcgtgtgctg catcaattac atttttgatc aaacgtaaaa ttgatgggtt atatggttgg
    29761 tacaagtaag aaacgcgttc gttcatacgg tctgctgcca ttgtgtattg aatcaaatcg
    29821 ttcgtaccta cagagaagaa atctacttct tttgcaaact tgtctgctaa gactgctgct
    29881 gctggaatct cgatcatgat gccgacttgg atcgtatcag atacttcctt gccttcactt
    29941 atcaattttt gtttttcttc ttcaaagatc gcttttgctg cgcggaattc tttgagtgta
    30001 gctaccatag ggaacatgat acgtaaatta ccatgaacag atgcacgaag caatgcacgc
    30061 atttgtgtac ggaacatttc gtcgccttgt tcagataaac tgatacgcaa tgcacgatat
    30121 cctaagaatg gattcatttc atgaggtaat tgcagatatg gtagttcttt atctccaccg
    30181 atgtccattg tacgaacaac tacaggtttt ccttccatac cttctaatac agctttgtaa
    30241 gcttcatatt ggtcatcttc tgttgggaag tctggagaat ccatatacag gaattcagta
    30301 cggtacagac caactgcttc tccaccatta ttatggacac caactagatc ttttggtgtt
    30361 ccgatgtttg cagctaattc aaaatgcttg ccgtcagctg tgacagtttc tgcatttttt
    30421 aatttttccc attctgcttt ttgtgctgcg taatctgcac ctgctttttc aaattcagct
    30481 ttttgttcat ctgtaggatc gatgattaca tcgccttcga tcccgttgac agccaaaata
    30541 tcgccttctt tgactttagc agtaatttct tttgtcccaa cgattgctgg aatttctaaa
    30601 gaacgtgcca tgatagcaga atgtgaagta cgtccaccaa tgtctgttac aaacgctttt
    30661 acaaaattgc gatctaattg tgctgtatca cttggagtca agtcatgtgc aacaacgacc
    30721 acttcttcgt tgatcattga tggatttggt aaagtaacgc ctaataagtg cgctaaaata
    30781 cgtttagcaa cgtcacgaat atctgcagca cgctcttgca tataagcatt atcttccatt
    30841 gcttcgaaca taccgatata catatccgtt acttctttta gcgctgattc agcatttacg
    30901 ctgttgtctt taatattttg cttgatttga cctaccattt ctggatctga aagaaccatc
    30961 aaatgggcat caaatacttg cgcttctgct tcacctaagc tttgcgctgc tttttcacga
    31021 atttgctgca attcttcagt agatttagct aatgcagcat ccaaacgagc ttcttcagcc
    31081 gcagtatctt ctactgttgc cttactgaat gttaaatccg gttgaactag caggtaagct
    31141 tttgcaacgg ctacaccgtc actagcggca atccctttta gcatttcaac cattattcag
    31201 ataagccttc tttcttcatt gtttcaacga ttgctgccat tgcatcagct tcgtcagcac
    31261 cttctgcagt aatagtaaca tcagaacctt gacctacacc taaagacata acgcccatga
    31321 ttgatttcaa gtttactgat ttacctttat actctaagtt tacatcagag ttaaatttgc
    31381 ttgctgtttg tactaatagt gtagctggac gagcgtggat cccagtttct gctactacgt
    31441 gaaattcttt cttttccata ttaatcggtc tcctttaagt gagtgttgtt tttatgtgag
    31501 ggttttcttt tttagtaatt cagagaatcc taacaaatgc caccctttca tctgtaaaga
    31561 ttaacatttt ttttcgttag atacaacagt tttcattcaa tttcagataa aagtattcaa
    31621 attattccgg attctttttc atccttttct tcacatttta taacaaacgt gtttatttcc
    31681 tttttcttgt cgctttctct tctcctgacg tttatcattc tttcctttga ccctctttgt
    31741 tttcatatga aacgaattac ttgcttccct ttttattttt agtataataa gagtcaaagg
    31801 tcaggaatgg tcaatagaga tttcaccttt tctacctcat aaatctctta aaaaactagg
    31861 ggttattctt ttacctcatg aaaaacgaga tgtaaaatat ggatgtaccc atttttgtga
    31921 aaggacgtga atgattcatg ctttgtcaaa actgcggcaa gaatgaggca acgattcact
    31981 tgtatgccac tgtcaatggt cagcggacac aactcgatta ctgtcagagc tgttatcaaa
    32041 aaatcaaaaa tcaacaaaat ggaggtctga ctaatatggc ccaaaatgat ccatttggat
    32101 ttggcagttt ggacgatctt ttccgttcca tgtctcgcca aatgcaacag caaggaaatt
    32161 atgagcaaac accaccaaca caatttggcg gaaacaacaa cttcaatggt ggtcagccac
    32221 ctcaaggcaa tacagaaggt ttattgggag aatacggaat caatatcaca gaacaagcac
    32281 gacaaggtga tatcgatcct gtgatcggac gcgatgacga aatcaaacga gtcattgaaa
    32341 ttttaaatcg ccgaaccaag aacaatcctg tcttgattgg agaaccaggt gttggtaaaa
    32401 cagcagttgt tgaaggttta gcacaaaaaa tcgtagatgg tgatgtaccg caaaaattaa
    32461 tggacaaaga agtcattcgt ttagatgtcg tatctttagt ccaaggtact ggtatacgag
    32521 gacaatttga agaacgtatg caaaaattga ttgaagagat ccgtcaagca gaaaatgtga
    32581 tcctattcat agatgaagtt cacgaaatag tcggtgctgg ttctgctggt gatggaaata
    32641 tggatgctgg taatatttta aaaccagctt tagctcgagg agaattgcaa atggttggtg
    32701 ctactacctt aaatgagtac cgcatcatcg aaaaagacgc tgctttagaa cgtcgtatgc
    32761 aacctgtacg ggtagatgaa ccaactgtcg aagaaacgat cagtattttg aaaggtttgc
    32821 aaaaacgtta tgaagactat catcatgtaa aatatactga tgaggccatc gaagcagccg
    32881 caactttatc caatcgttac gtccaagatc gcttcttgcc ggataaagcc atcgatttgc
    32941 tagatgaaac aggttctaag aaaaacttga cgatccaaat cgttgatcct aaaacaatcg
    33001 agaaaaaact gcaagaagca gaagaacaaa aagttttagc ttctcgagaa gaagatttcg
    33061 aaaaagcagc ttattatcgc gatcagatca ataaacttca aaaaatgaaa gaacgtcagc
    33121 tcactgaaga ggaaacacct gtgattaccg aaaaagatat ggaaaaaatc gttgaacaac
    33181 ggacaggtat tccagtgggt gaattaaaag aaaaagaaca gacacaactg aaaaacttag
    33241 cagatgactt gaaagctcat gtcatcggac aagataacgc tgtagatcga gtagcgaaag
    33301 cgattcgccg aaaccgtgtt ggattaaaca aacagaatcg tccaatcggc tccttcttat
    33361 ttgttggacc aactggtgta ggtaaaacag agcttgcgaa acaacttgcc tacgaactat
    33421 tcggttcaca agattcaatg attcgtttcg atatgtctga atatatggaa aaacatagtg
    33481 tctctaaatt gattggttct cctccaggtt atgtcggata cgaagaagct ggtcaattaa
    33541 cagaaaaagt acggagaaat ccttacagtc ttgtcttgtt agatgaagta gaaaaagctc
    33601 atccagatgt tttgcatatg ttcttgcaaa tcttagatga tggtcgattg acagatgcac
    33661 aaggaagaac ggtcagcttt aaagatacga tcatcattat gacaagtaat gccggaactg
    33721 gtaaagtaga agcaaacgtt ggttttggtg cggcacgcga aggtgttact cgttctgtat
    33781 tgaaccaatt aaacaactac tttacaccag aatttctgaa ccgttttgac gggatcatcg
    33841 aattcagtgc attatcaaaa gaaaacttga tgacaatcgt cacactaatg ctagacgatg
    33901 tcaaccaaat gctggcagca cagcaacttc acatcgaagt tcctacgaat gtcaaagaaa
    33961 aattagttga cttaggctat gatccatcta tgggtgctcg tcctcttcga cgcacgatcc
    34021 aagaacaaat cgaagatggt atcgctgaat tttatcttga tcacccttct atccatgagt
    34081 tgaaagcaaa actggataaa gatggaaaaa tcatcgttac ttctaaacca gaacgtttgg
    34141 ctaaagaatc agccgaagaa acggctgaat aaatccagtt ttatagcgtg agacggtatt
    34201 tctttgagaa atgccgtctc attctttttt cagaaaagtc aaaatactgt tcattttata
    34261 aaaaagaaat tataataaga gaaacaagca attaaaaata cataaaagag gtgagcgcca
    34321 tgaaattggt aaatgtaaca aacagccatt cccgtcttgt attaaaccag cttgaaaaca
    34381 ctgatgccca tctagtcaag gtctacacag ccggaaatac cactattgtc tacactgaag
    34441 cccctcaaca taatgaaatt ctgctgatca atgacaaacg taaaatccag ccgaaagaaa
    34501 tcgaagatgc taaaaattat ttcttagata aaatcaaaga cgccgtttac cacgaggatg
    34561 aaatcaaagt aatcgaattg gcaggccttg ttgaaatctc gattccaaaa catgaattag
    34621 gcgctacatt agccgtgtaa acaaattttg aacataataa ttaaagagcg tgggacaaaa
    34681 gtaaa
//
LOCUS       DAYYBG010000019        34510 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000019 DAYYBG010000000
VERSION     DAYYBG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.019"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHAUN_000952"
     CDS             212..604
                     /locus_tag="ACHAUN_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8459675.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHAUN_000953"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHAUN_000953"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8459676.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHAUN_000954"
     CDS             2698..2943
                     /locus_tag="ACHAUN_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459677.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHAUN_000955"
     CDS             3165..4775
                     /locus_tag="ACHAUN_000955"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8459678.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHAUN_000956"
     CDS             complement(4752..4964)
                     /locus_tag="ACHAUN_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459679.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHAUN_000957"
     CDS             5067..5936
                     /locus_tag="ACHAUN_000957"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8459680.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHAUN_000958"
     CDS             6101..7372
                     /locus_tag="ACHAUN_000958"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8459681.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHAUN_000959"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHAUN_000959"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8459682.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHAUN_000960"
     CDS             8763..9026
                     /locus_tag="ACHAUN_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8459683.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHAUN_000961"
     CDS             9471..9920
                     /locus_tag="ACHAUN_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8459684.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHAUN_000962"
     CDS             9977..11395
                     /locus_tag="ACHAUN_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8459685.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHAUN_000963"
     CDS             11395..12255
                     /locus_tag="ACHAUN_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8459686.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHAUN_000964"
     CDS             12429..13202
                     /locus_tag="ACHAUN_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8459687.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHAUN_000965"
     CDS             13349..13714
                     /locus_tag="ACHAUN_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8459688.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHAUN_000966"
     CDS             complement(13862..15070)
                     /locus_tag="ACHAUN_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8459689.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHAUN_000967"
     CDS             complement(15089..15367)
                     /locus_tag="ACHAUN_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459690.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHAUN_000968"
     CDS             15560..16216
                     /locus_tag="ACHAUN_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8459691.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHAUN_000969"
     CDS             complement(16658..17455)
                     /locus_tag="ACHAUN_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8459692.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHAUN_000970"
     CDS             complement(17452..18636)
                     /locus_tag="ACHAUN_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8459693.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHAUN_000971"
     CDS             complement(18661..19908)
                     /locus_tag="ACHAUN_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8459694.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHAUN_000972"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHAUN_000972"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8459695.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHAUN_000973"
     CDS             21888..22202
                     /locus_tag="ACHAUN_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8459696.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHAUN_000974"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHAUN_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8459697.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHAUN_000975"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHAUN_000975"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8459698.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHAUN_000976"
     CDS             23546..23875
                     /locus_tag="ACHAUN_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8459699.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHAUN_000977"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHAUN_000977"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8459700.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHAUN_000978"
     CDS             24863..25687
                     /locus_tag="ACHAUN_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8459701.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHAUN_000979"
     CDS             25680..26027
                     /locus_tag="ACHAUN_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8459702.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHAUN_000980"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHAUN_000980"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8459703.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHAUN_000981"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHAUN_000981"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8459704.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHAUN_000982"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHAUN_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8459705.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHAUN_000983"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHAUN_000983"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8459706.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHAUN_000984"
     CDS             31684..32451
                     /locus_tag="ACHAUN_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459707.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHAUN_000985"
     CDS             32441..34483
                     /locus_tag="ACHAUN_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459708.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11095 a         6120 c         7248 g        10047 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat
//
LOCUS       DAYYBG010000020        32497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000020 DAYYBG010000000
VERSION     DAYYBG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.020"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACHAUN_000986"
     CDS             complement(<1..563)
                     /locus_tag="ACHAUN_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFM8459709.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACHAUN_000987"
     CDS             complement(750..1400)
                     /locus_tag="ACHAUN_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459710.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACHAUN_000988"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACHAUN_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8459711.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACHAUN_000989"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACHAUN_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8459712.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACHAUN_000990"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACHAUN_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8459713.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACHAUN_000991"
     CDS             complement(4926..5657)
                     /locus_tag="ACHAUN_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8459714.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACHAUN_000992"
     CDS             complement(5661..6926)
                     /locus_tag="ACHAUN_000992"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8459715.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACHAUN_000993"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACHAUN_000993"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8459716.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACHAUN_000994"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACHAUN_000994"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8459717.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACHAUN_000995"
     CDS             12268..13770
                     /locus_tag="ACHAUN_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8459718.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACHAUN_000996"
     CDS             13772..14509
                     /locus_tag="ACHAUN_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8459719.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACHAUN_000997"
     CDS             complement(14680..15873)
                     /locus_tag="ACHAUN_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8459720.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACHAUN_000998"
     CDS             complement(15952..16158)
                     /locus_tag="ACHAUN_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8459721.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACHAUN_000999"
     CDS             complement(16151..17293)
                     /locus_tag="ACHAUN_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8459722.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACHAUN_001000"
     CDS             17805..18440
                     /locus_tag="ACHAUN_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8459723.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACHAUN_001001"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACHAUN_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACHAUN_001002"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACHAUN_001002"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACHAUN_001003"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACHAUN_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFM8459724.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="ACHAUN_001004"
     CDS             21086..21253
                     /locus_tag="ACHAUN_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8459725.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="ACHAUN_001005"
     CDS             complement(21313..21666)
                     /locus_tag="ACHAUN_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8459726.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="ACHAUN_001006"
     CDS             22170..22589
                     /locus_tag="ACHAUN_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8459727.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="ACHAUN_001007"
     CDS             22615..22821
                     /locus_tag="ACHAUN_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8459728.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="ACHAUN_001008"
     CDS             complement(23049..23222)
                     /locus_tag="ACHAUN_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459729.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23234..23863
                     /locus_tag="ACHAUN_001009"
     CDS             23234..23863
                     /locus_tag="ACHAUN_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8459730.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACHAUN_001010"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACHAUN_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8459731.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACHAUN_001011"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACHAUN_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8459732.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACHAUN_001012"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACHAUN_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8459733.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACHAUN_001013"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACHAUN_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8459734.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACHAUN_001014"
                     /pseudo
     CDS             complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACHAUN_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(30257..30484)
                     /locus_tag="ACHAUN_001015"
     CDS             complement(30257..30484)
                     /locus_tag="ACHAUN_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8459735.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31575
                     /locus_tag="ACHAUN_001016"
                     /pseudo
     CDS             30968..31575
                     /locus_tag="ACHAUN_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            31748..32461
                     /locus_tag="ACHAUN_001017"
     CDS             31748..32461
                     /locus_tag="ACHAUN_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459736.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9520 a         6618 c         5736 g        10623 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt
    31741 attttatatg aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg
    31801 tacgtctcct agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag
    31861 acaaaccagt tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac
    31921 atcttcaaca agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt
    31981 tgagacaaat atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat
    32041 agatccgtta gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat
    32101 tagaggtaat acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc
    32161 tgcaggaaca cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt
    32221 tatgctcact gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg
    32281 gacaatcact agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta
    32341 tccagcgttc attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat
    32401 tcctacagga aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta
    32461 gactttctaa tatgttatac aaaaagaagc tgacagc
//
LOCUS       DAYYBG010000021        30459 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000021 DAYYBG010000000
VERSION     DAYYBG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.021"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACHAUN_001018"
     CDS             complement(451..1761)
                     /locus_tag="ACHAUN_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8459737.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="ACHAUN_001019"
     CDS             complement(1773..3131)
                     /locus_tag="ACHAUN_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8459738.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3333..4901)
                     /locus_tag="ACHAUN_001020"
     CDS             complement(3333..4901)
                     /locus_tag="ACHAUN_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8459739.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4905..5315)
                     /locus_tag="ACHAUN_001021"
     CDS             complement(4905..5315)
                     /locus_tag="ACHAUN_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8459740.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5312..6235)
                     /locus_tag="ACHAUN_001022"
     CDS             complement(5312..6235)
                     /locus_tag="ACHAUN_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8459741.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6232..7110)
                     /locus_tag="ACHAUN_001023"
     CDS             complement(6232..7110)
                     /locus_tag="ACHAUN_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8459742.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7097..8434)
                     /locus_tag="ACHAUN_001024"
     CDS             complement(7097..8434)
                     /locus_tag="ACHAUN_001024"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8459743.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8437..9540)
                     /locus_tag="ACHAUN_001025"
     CDS             complement(8437..9540)
                     /locus_tag="ACHAUN_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8459744.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9614..11122)
                     /locus_tag="ACHAUN_001026"
     CDS             complement(9614..11122)
                     /locus_tag="ACHAUN_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8459745.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11088..11780)
                     /locus_tag="ACHAUN_001027"
     CDS             complement(11088..11780)
                     /locus_tag="ACHAUN_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8459746.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11802..12776)
                     /locus_tag="ACHAUN_001028"
     CDS             complement(11802..12776)
                     /locus_tag="ACHAUN_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459747.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13076..13948
                     /locus_tag="ACHAUN_001029"
     CDS             13076..13948
                     /locus_tag="ACHAUN_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459748.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14406..16604)
                     /locus_tag="ACHAUN_001030"
     CDS             complement(14406..16604)
                     /locus_tag="ACHAUN_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8459749.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16836..17828
                     /locus_tag="ACHAUN_001031"
     CDS             16836..17828
                     /locus_tag="ACHAUN_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8459750.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17888..19309
                     /locus_tag="ACHAUN_001032"
     CDS             17888..19309
                     /locus_tag="ACHAUN_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8459751.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19302..20801
                     /locus_tag="ACHAUN_001033"
     CDS             19302..20801
                     /locus_tag="ACHAUN_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8459752.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20840..22234
                     /gene="uxaC"
                     /locus_tag="ACHAUN_001034"
     CDS             20840..22234
                     /gene="uxaC"
                     /locus_tag="ACHAUN_001034"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8459753.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22393..23076
                     /locus_tag="ACHAUN_001035"
     CDS             22393..23076
                     /locus_tag="ACHAUN_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8459754.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23078..23914
                     /locus_tag="ACHAUN_001036"
     CDS             23078..23914
                     /locus_tag="ACHAUN_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8459755.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23925..25679
                     /locus_tag="ACHAUN_001037"
     CDS             23925..25679
                     /locus_tag="ACHAUN_001037"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459756.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25955..26296)
                     /locus_tag="ACHAUN_001038"
     CDS             complement(25955..26296)
                     /locus_tag="ACHAUN_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8459757.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26364..28535)
                     /locus_tag="ACHAUN_001039"
     CDS             complement(26364..28535)
                     /locus_tag="ACHAUN_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8459758.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28746..29603
                     /locus_tag="ACHAUN_001040"
     CDS             28746..29603
                     /locus_tag="ACHAUN_001040"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8459759.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29674..>30459)
                     /locus_tag="ACHAUN_001041"
     CDS             complement(29674..>30459)
                     /locus_tag="ACHAUN_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8459760.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9099 a         6274 c         5391 g         9695 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact agtgaaaata
     3301 agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc atcatacttg
     3361 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
     3421 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
     3481 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
     3541 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
     3601 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
     3661 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
     3721 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
     3781 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
     3841 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
     3901 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
     3961 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
     4021 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
     4081 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
     4141 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
     4201 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
     4261 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
     4321 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     4381 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     4441 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     4501 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     4561 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     4621 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     4681 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     4741 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     4801 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     4861 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     4921 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     4981 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     5041 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     5101 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     5161 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     5221 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     5281 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     5341 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     5401 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     5461 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     5521 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     5581 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     5641 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     5701 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     5761 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     5821 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     5881 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     5941 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     6001 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     6061 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     6121 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     6181 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     6241 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     6301 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     6361 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     6421 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     6481 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     6541 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     6601 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     6661 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     6721 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     6781 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     6841 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     6901 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     6961 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     7021 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     7081 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     7141 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     7201 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     7261 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     7321 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     7381 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     7441 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     7501 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     7561 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     7621 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     7681 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     7741 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     7801 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     7861 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     7921 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac
     7981 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     8041 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     8101 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     8161 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     8221 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     8281 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     8341 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     8401 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     8461 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     8521 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     8581 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     8641 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     8701 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     8761 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     8821 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     8881 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     8941 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     9001 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     9061 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     9121 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     9181 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     9241 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     9301 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     9361 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     9421 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     9481 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     9541 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     9601 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     9661 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     9721 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     9781 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     9841 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     9901 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     9961 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
    10021 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
    10081 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
    10141 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
    10201 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
    10261 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
    10321 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
    10381 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
    10441 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
    10501 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
    10561 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
    10621 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
    10681 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
    10741 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
    10801 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
    10861 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
    10921 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
    10981 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
    11041 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
    11101 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
    11161 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
    11221 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
    11281 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
    11341 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
    11401 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
    11461 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
    11521 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
    11581 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
    11641 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
    11701 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
    11761 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
    11821 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
    11881 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
    11941 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
    12001 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
    12061 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
    12121 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
    12181 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
    12241 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
    12301 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
    12361 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
    12421 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
    12481 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
    12541 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
    12601 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
    12661 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
    12721 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
    12781 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
    12841 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
    12901 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
    12961 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
    13021 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
    13081 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
    13141 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
    13201 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
    13261 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
    13321 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    13381 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    13441 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    13501 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    13561 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    13621 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    13681 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    13741 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    13801 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    13861 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    13921 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    13981 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    14041 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    14101 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    14161 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    14221 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    14281 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    14341 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    14401 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14461 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14521 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14581 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14641 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14701 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14761 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14821 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14881 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14941 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    15001 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    15061 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    15121 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15181 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15241 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15301 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15361 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15421 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15481 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15541 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15601 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15661 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15721 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    15781 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15841 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15901 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15961 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    16021 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    16081 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    16141 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt
    16201 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16261 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16321 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16381 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16441 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16501 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16561 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16621 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16681 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16741 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16801 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta
    16861 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16921 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16981 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    17041 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    17101 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    17161 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17221 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17281 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17341 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17401 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17461 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17521 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17581 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17641 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17701 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17761 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17821 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17881 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17941 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    18001 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    18061 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    18121 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18181 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18241 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18301 acagagcctc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18361 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18421 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18481 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18541 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18601 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18661 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18721 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18781 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18841 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18901 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18961 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    19021 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    19081 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    19141 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19201 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19261 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19321 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19381 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19441 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19501 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19561 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19621 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19681 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19741 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19801 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19861 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19921 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19981 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    20041 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    20101 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    20161 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20221 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20281 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20341 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20401 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20461 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20521 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20581 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20641 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20701 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20761 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20821 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20881 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20941 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    21001 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    21061 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    21121 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21181 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21241 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21301 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21361 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21421 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21481 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21541 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21601 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    21661 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21721 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21781 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21841 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21901 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21961 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    22021 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    22081 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    22141 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22201 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22261 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22321 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    22381 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    22441 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    22501 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    22561 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    22621 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    22681 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    22741 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    22801 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    22861 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    22921 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    22981 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    23041 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    23101 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    23161 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    23221 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    23281 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    23341 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    23401 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    23461 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    23521 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    23581 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    23641 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    23701 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    23761 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    23821 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    23881 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    23941 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    24001 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    24061 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    24121 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    24181 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    24241 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    24301 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    24361 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    24421 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    24481 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    24541 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    24601 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    24661 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    24721 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    24781 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    24841 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    24901 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    24961 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    25021 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    25081 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    25141 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    25201 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    25261 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    25321 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    25381 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    25441 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    25501 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    25561 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    25621 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    25681 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    25741 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    25801 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    25861 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    25921 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    25981 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    26041 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    26101 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    26161 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    26221 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    26281 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    26341 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    26401 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    26461 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    26521 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    26581 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    26641 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    26701 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    26761 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    26821 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    26881 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    26941 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    27001 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    27061 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    27121 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    27181 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    27241 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    27301 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    27361 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    27421 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    27481 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    27541 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    27601 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    27661 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    27721 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    27781 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    27841 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    27901 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    27961 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    28021 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    28081 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    28141 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    28201 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    28261 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    28321 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    28381 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    28441 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    28501 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    28561 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    28621 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    28681 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    28741 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    28801 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    28861 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    28921 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    28981 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    29041 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    29101 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    29161 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    29221 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    29281 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    29341 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    29401 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    29461 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    29521 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    29581 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag cagataatga
    29641 taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa
    29701 cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga tatattcact
    29761 tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac ggattttttg
    29821 aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag gtgctaacgt
    29881 caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc aaacaggatg
    29941 atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc
    30001 taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt catctggatg
    30061 gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt
    30121 gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac tattttttcc
    30181 ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg ttgtccaagc
    30241 agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg cccacccttt
    30301 tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac caatcgggcc
    30361 gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa atacctgatc
    30421 caatgtttct tctggtgttg tggtagggat acttaattt
//
LOCUS       DAYYBG010000022        28983 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000022 DAYYBG010000000
VERSION     DAYYBG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.022"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..712
                     /locus_tag="ACHAUN_001042"
     CDS             <1..712
                     /locus_tag="ACHAUN_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFM8459761.1"
                     /translation="GVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAI
                     DGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDD
                     QSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDA
                     TPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSI
                     LGVLLVFISIKFRKKRNI"
     gene            complement(1065..1913)
                     /locus_tag="ACHAUN_001043"
                     /pseudo
     CDS             complement(1065..1913)
                     /locus_tag="ACHAUN_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2066..3178
                     /locus_tag="ACHAUN_001044"
     CDS             2066..3178
                     /locus_tag="ACHAUN_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8459762.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3256..3549)
                     /locus_tag="ACHAUN_001045"
     CDS             complement(3256..3549)
                     /locus_tag="ACHAUN_001045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8459763.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3717..4031
                     /locus_tag="ACHAUN_001046"
     CDS             3717..4031
                     /locus_tag="ACHAUN_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8459764.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4052..6031
                     /locus_tag="ACHAUN_001047"
     CDS             4052..6031
                     /locus_tag="ACHAUN_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8459765.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6044..6370
                     /locus_tag="ACHAUN_001048"
     CDS             6044..6370
                     /locus_tag="ACHAUN_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8459766.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6386..6904
                     /locus_tag="ACHAUN_001049"
     CDS             6386..6904
                     /locus_tag="ACHAUN_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459767.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6904..8256
                     /gene="celB"
                     /locus_tag="ACHAUN_001050"
     CDS             6904..8256
                     /gene="celB"
                     /locus_tag="ACHAUN_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8459768.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8340..9845)
                     /locus_tag="ACHAUN_001051"
     CDS             complement(8340..9845)
                     /locus_tag="ACHAUN_001051"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8459769.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9937..10527)
                     /locus_tag="ACHAUN_001052"
     CDS             complement(9937..10527)
                     /locus_tag="ACHAUN_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8459770.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10706..12352
                     /locus_tag="ACHAUN_001053"
     CDS             10706..12352
                     /locus_tag="ACHAUN_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM8459771.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12401..13120
                     /locus_tag="ACHAUN_001054"
     CDS             12401..13120
                     /locus_tag="ACHAUN_001054"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8459772.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13599..14546
                     /locus_tag="ACHAUN_001055"
     CDS             13599..14546
                     /locus_tag="ACHAUN_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459773.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14558..15478
                     /locus_tag="ACHAUN_001056"
     CDS             14558..15478
                     /locus_tag="ACHAUN_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459774.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15519..16976
                     /locus_tag="ACHAUN_001057"
     CDS             15519..16976
                     /locus_tag="ACHAUN_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8459775.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17321..17935
                     /locus_tag="ACHAUN_001058"
     CDS             17321..17935
                     /locus_tag="ACHAUN_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8459776.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17928..19652
                     /locus_tag="ACHAUN_001059"
     CDS             17928..19652
                     /locus_tag="ACHAUN_001059"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8459777.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19649..21100
                     /locus_tag="ACHAUN_001060"
     CDS             19649..21100
                     /locus_tag="ACHAUN_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8459778.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21573..23726)
                     /locus_tag="ACHAUN_001061"
     CDS             complement(21573..23726)
                     /locus_tag="ACHAUN_001061"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8459779.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23728..24774)
                     /locus_tag="ACHAUN_001062"
     CDS             complement(23728..24774)
                     /locus_tag="ACHAUN_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8459780.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24924..26213
                     /locus_tag="ACHAUN_001063"
     CDS             24924..26213
                     /locus_tag="ACHAUN_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8459781.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26226..28928
                     /locus_tag="ACHAUN_001064"
     CDS             26226..28928
                     /locus_tag="ACHAUN_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8459782.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9421 a         5008 c         5869 g         8685 t
ORIGIN      
        1 cggtgtcgtg ttgacaaaga tcgatgacca aagcggggaa gtcctgcaag gagctgtttt
       61 tgagttacaa gatgccaatg gaaccgttct gcagtcaggt ctaaccacag gagcagatgg
      121 aaaactggcg attgacggct tagcaccagg agtgtatcag ctggtagaaa cccaagcgcc
      181 aacggggtat gaattggatg cgacaccaat cgagtttgag atcgaaagaa gtcagacagc
      241 ggtggtagaa ttgacgaaag aaaatcgact tacaccaggc ggtgtcgtgt tgacaaagat
      301 cgatgaccaa agcggagaag tcctgcaagg agctgttttt gagttacaag atgccaatgg
      361 aaccgttctg cagtcaggtc taaccacagg agcagatgga aaactggcga tagatggctt
      421 agcaccagga gcatatcagc tggtagaaac tcaagcgcca acggggtatg aattggatgc
      481 gacaccagta actttcaaga tcgaaaaaga acaagaagtg gcgatctttt taacaaaaga
      541 gaatagaaaa gttgctgata gctcagatat aagaggaaac aatccaacaa gcagtggaaa
      601 taagcatctt ccaaaaactg gagaaaccat cattagccag tttatattgt caatattggg
      661 tgtgctactt gttttcatca gtataaaatt tagaaagaaa cgaaatatat gagctaaaat
      721 aaaaaattga ataagcatag tttttaaaat caactagtac ccatcaattg aatggaaact
      781 accgatttta gaaggggtat acaaacaaat attagtatcg gagtagagaa ggttgaaccc
      841 aaccagtaaa taagtaaaga caattttgtg ctgcgttgag aagtgaaaaa taaacctttg
      901 tagtaatact agttaataaa aaatatacaa aaacactgcg aaaatcattc ttgagtttcg
      961 cagtgttttt tgcctaatga ctcaagttta ttgggtaata ctttgatttt ctttctacaa
     1021 ataatcagcg atcagttgta attttttctc tagttttaat ggctctttgt caataaggag
     1081 aaaaacaagt cgggtctttt gtctcggaaa gcttcaacca actcgtgaat aaaggttacg
     1141 cctccttgaa aggcgtggaa aaaccaagta atcgatcaat caccaatttc gcatttggta
     1201 gaacatgttt ggtgagctga aagtaggtgg cgttcatgtc tgtaactaag aattctattt
     1261 ctttaggatt agtgcagcta aggaaatagt ttgttaatcg aggaattttt tgtgtaggta
     1321 aattttctcg attaattttt ctgtctcgcc atccgcggaa ataaaactca gtttatcctc
     1381 aacggaaacg tgagaacgaa attcacaacc ctcaacactc aagacagaat tcttttggaa
     1441 gactttggta aatagcttct caactctttc aatgtgcgaa taacggttgt caaagatacc
     1501 taacaacttt tcgaaataaa aaataacgac actttttcag tcagtaaaga agtgatttta
     1561 tgtctgacat gatttgaaat cgagtgcctt ggacggataa aataactttg agcgttccaa
     1621 tggcttcgac agtttttaca ggtatagcgc tgctttttta ggcgtataac cattggcata
     1681 tgattatatt attcaaagtg gacaatggtt tccttcttct tgtttttcac caccattaat
     1741 ttcctatttc catctaccac agcagaacca caatttctac agacacatgg agcagggaag
     1801 agaactgcag caacgatcaa tgtttttttc ttctgtaagg tttcgtaaga gacctctgta
     1861 atcatcaaat ttttgtctat tgatctcaga atttttttga tagaaccatt cataaagcat
     1921 atcgttctct cagttgttta ttttgtggtg atttaatcat acaagaaaac gatatgtttt
     1981 tttataaaaa catgaatagg aaaaatagtt gacaagaaaa tgacaacgcc atatgatgtt
     2041 ccatagtatt tacaacctgt catagatgac tgtaaaaaat aaaagtaaaa agaaaggacg
     2101 acaacaggtg gatttttatc agtctctaca actagatccg tttattttaa aacaaaaaat
     2161 acgagaagca gcttctaaaa aagaaaagtg catgtatatt tgttcattgt ttttacgatc
     2221 attattcatc gtattgtttg ccatttgttt tatcattata attacaacat tatttgaatc
     2281 gaagcataaa ccatacgctg tggttttatt ctgtatgttg atgagtattc gttttgttga
     2341 ctttggttat aagatttccc attcgatcat aggtttagca atcgtcatgt tctcactatt
     2401 agtagcaccg tatgtccagc tgatcaaatg gtcagttatg ggcatgttga ttcattttat
     2461 tttactaagc agcatattaa tggcaaccgc tagtgatccc aaaatgggga atgcttcttt
     2521 gtacggcttt tcatatctct ttattgttta ttcgttacct aaagattcgt tgaacaagaa
     2581 tttctttaca caaacaagta gcttattatt tttgtttttt tgttggtttt cagttctcct
     2641 ttacagaaaa catcgagaaa agaacaaaga caaaagcttg tttaaagaga tttttttgaa
     2701 aggcatgtat tcgcaggaaa aaatttggat gctcatctat gcatttggca ttagtctttt
     2761 gatcgttgca ggagagtatg tcccttttca gcgattgatg tgggcagggt ttgcattttc
     2821 ttctattgta tctagctatg gattgatgtc gattggtttc aaagaacgag cagtagaccg
     2881 aatcattggc tcattgattg gatgtgtttt atttattggt atcagccaat ttattccttt
     2941 taactgggtc ggtatattag gtggtctagc tttaggtgtt tgttctacct atagatataa
     3001 aacagttttt aattcttttg gtgctttggc aatcactgcc agtttattcg gggttccagg
     3061 agcagtaacg atccgcattt ttgagaatat ccttggggta tgtttaggaa tcatgtatat
     3121 tggggtgact gaaatactta tccgaaaaat aagagaaaaa cacggtttga atcattagat
     3181 gagatcgaat aaaaaataag gaaaaagtga agatacacat ggatgataaa ttgctatcat
     3241 tccatgtgta tctttttacg gtaaatcagt gaaacgttcg actttcattt ttaatttata
     3301 ctttttacgg agttcgttta tttgagccat catttcggat ttgtgatgct tttcgattgc
     3361 ttcttcatct ttccaacggt cgatcagtaa aagagattct ggatcttcta gtggaaagta
     3421 atagtcatat ttctcgtttc cttcttctgc acgaatcgct ttgacaatgt ttctttcgac
     3481 catttcttct gcaaattttc tggcttgtcc atttgtactt gtatacaaaa tattcactgt
     3541 gatactcact aaatcccctc ctgaaaaaat tctatcacta gtatatcacg gtgccagaaa
     3601 ttattattca agatatgata cacatttttt ccgcaatgct acggaaatat atattttgaa
     3661 tagaaaacgc ttacaagata tgcttggttt ataaaaataa aaggagtgat tgatttatga
     3721 aaaaagcatt gattatttgt gcggcaggaa tgtcatcctc gatgatggcc tcaaagacaa
     3781 cggatttctt taagcaaaaa agagaagagg tactcgtaga tgcagtttca gctacagaag
     3841 gcgataatat gattaagaat agtgattttg atttatttct gatcagcccg caaacgacga
     3901 tgtttttgga taaattcaaa aaattaggag aatctgttgg aaagcctgtt gttagtatcc
     3961 ctttccaagc gtatgtccca atccctacag gcatccagaa gttagcagag gtgatcgaag
     4021 agaacatatg atggaatagg agtggatgat aatgaaacag aaggaaaaat tgctgttgag
     4081 atatttgatt gatcacaaga aggagtatgt caccagtcaa cgtttagctt ctgaattgtt
     4141 attatctgat cggacaatcc gtaattactt gcagcggatc aaagagttag tagaaaaaaa
     4201 cggaggtaag atcattgcta aaccaggtta tggctaccaa ctgcatattt tacaaagact
     4261 gacatttgac ttgtttttga gtcggcaaga aatagtacct ggatatgcta gagaaccgca
     4321 aggattttat gagtccgaag atcgacaaaa gtatatattg aaccaattgc ttttggaaga
     4381 tgccattctt tttatggatg atttggcaga agagctttat atcagtcgtt ccagtttgac
     4441 aaaggacatg caagaaatca aggagcgatt gattccatat tcactaaaaa tcgtttctaa
     4501 gcatggtcaa gggacatgga tcgaagggga agaaagaaat cgacggcatt ttatcatgga
     4561 tacttttttt ggaaaccatt atggaaattc attgaaagaa tatttagggc aaagccgttt
     4621 tttcccagac atcagttttg aagagctagt cattatcatt ctcgatgaaa caagagaagc
     4681 taagttaaaa gtttcggatt ttatcataca gaatctgact ttacacttgg ctttggggat
     4741 caaacggctc cgagaagggt ttgaaatcaa ggaattaggc atcgaacaag aagtttctaa
     4801 gcgagtagag tatcaagtgg cgcagcggat cgttcaacgt attgaggtaa tcgcaggtgt
     4861 atcgtttccc gaagaagaaa ttgcttattt gacattacat ctcatggcta agtcgaatca
     4921 caaagaaaat caaaatgatc aggaactatc aaaggaatta gcgaaagttc tgaaaagatt
     4981 gtctcaggaa tttcattttt cattagtaga ggatctccag cttttcaatg gactactcga
     5041 tcatttgtca ccgatgatga ttcgattgag ccgaggtatt cgcattgaga acccattgac
     5101 tgaggagata aagaaagaaa atcccaaagt ctttgacgca gtaaaaaggc atttcagcaa
     5161 catgccggct ttgaaaaact ataccatcaa tgaagatgaa tgggcctatt tggctttgca
     5221 tctcatggcg gcattggaaa aagaaagagc tgcgcataaa ctacatgcat tgattatctg
     5281 tgcaacgggt tacggcagtg cccaactatt aaagaatcgc gtagtcagtg aattcggtaa
     5341 aaatattaca gtggtaagtg taaaaggcta ttatgagatc aatgaaacaa ctttaaaagg
     5401 aatcgacctg attatttctt ctattgatct ttcgacaatg tttttcaaaa ttccagtttt
     5461 acatgtcagt atttttttga atgaagatga tgttcgtaaa atacgaaagg tagttggaga
     5521 atctataccc tatcattcct cagatagacc gataccagca ttttctctac agcaaaaaaa
     5581 gcagatttac accgaacagt tgtctgaaaa atttttcaag acttatacgt acgaaccgaa
     5641 aaaggaagaa attcttcatg atttgctgca aaagctgagc ataaatgaag aagaaggata
     5701 tataagagaa atgcgaaagc agatccagca acgtgaaaat atgggacaaa tcatattcag
     5761 tgatacagtg gcagtcccac atcccgctgt tccagttgga atcgaaacaa aggtagccgt
     5821 tgcattgatt ccttctggga tgcagtggga tcaatatcaa gaaattcatt ttgttttttt
     5881 agtttcgcct tcttatgtcg aaaatgaagg aattacggtc gtgactaaag caatcgttag
     5941 gttagttgac cagctaaaag tacagcagga aatcttagct gaacctattt ttgagaattt
     6001 cagcagtaaa tttatcaaac tgatcgaata aggaggaaaa cagatggctg aaaaaataaa
     6061 cagtgaaaca ctacaagtga cttcttttga tatcattttc catagcggaa atgcacggac
     6121 actgatacat gaagcatttc gctcgatgcg taaagagatg tttgaagaag tagaagagaa
     6181 gttgcgacaa gcaaatgaag agattcttca tgcgcatcag tcacaaactc aacttctgaa
     6241 agaatatgcc agcgggacaa aaatagaaat ggaaattatc atggtccatg cacaagatca
     6301 tctgatgacg acgatgacat tattggaagt tgcacaggaa atgaattatt tgtatcaaaa
     6361 aaataaatag aaaggaagga tcattgtgca ggatacagta aaaaggaaaa tcgaattaga
     6421 aaaaaagcaa cttagtttga aaaacatgta tttcaatcgt tatctaatcg tacgttacct
     6481 gactgccttt ttctttttca tgaacatgca atggatgatt ctgcttctat cagctaaaag
     6541 tttaggaagt agtttaccga tgattctgat tttagctatt ttgcctgcag taggagaaca
     6601 agtaaagctt tatcgaaaac accaaacgaa cgttccatgg acaaagtggt attttttgtt
     6661 ccagagtgtc tttaatttat tagtgattcc agtgttgttt acctcaggat ttacattctt
     6721 gtatcctttt atggcaaata acaatggagg acagttattt gttttcattc ttgttgtaag
     6781 cggtattttt gccagtgcat ttatacagta tcgtttaaag aaaatttcac tcaaccaaga
     6841 ccaacagtat atccgtatta aacaatatga aaaagcgtta tatttaggaa aggagaataa
     6901 ctaatgtttg attttttgca gaaatacctg atgggaccta tggggaaaat tgcgcaactt
     6961 aaaatcgttc gggctgtcat ggcagcgggg atggcttcta ttcctttcac gattgtaggc
     7021 tcgatgtttt tggttttaaa tattttgcca ttaccgttcc catttttaga aggatttttt
     7081 aatgcaactt ttttcaaagt tagtgacttg tacatgatcg tcaatacaat gacgatgggg
     7141 attttatcta tttactttgc cattgtgttt gcttatgaat taacgtcaat tgaacgagat
     7201 gaacaaaaat taaatgttaa tccgctcacg ggggcattac tatctgtttt tgcattcttt
     7261 atgtgtatcc ctgaattgat catgactgat ggtaaaatca gcttgattgc ttctatcaat
     7321 gagacagaaa ctgtagtaaa tggtattagg atgggctctt ttgtcgaacg tttaggaact
     7381 tcaggaatct ttacagctat tattatgtcg attattgcgg tcgaattgta ttgtatgtgt
     7441 gtcaagagaa attgggtcat aaaaatgcca gatgttgttc cgccaggagt ttctcgttct
     7501 ttcactgctc tgatcccaac atttgtcatt gcgtttgtgg tgatgatcat caatggatta
     7561 ttagttgcac taggaacgga tatcttcaaa atgattgcta tcccatttgg gtttgttacg
     7621 cagttgacga atacatggct tggtgtcatg gtgatttatt tcttgatcca tgcattgtgg
     7681 atcgtaggga tccatggagc gaatatcatc acttctttct tgaccccgat cgtattagct
     7741 aatatggcag ctaacgcaca aggcgcaaat tatccgttgg caggagaatt caacaattct
     7801 tatgtaaccg ttggtggatc aggtgcgact ttaggattga tcatctttat tgcattcctc
     7861 gcgaaatctg atcagctgaa agtgttagga aaagcctcac ttgtaccagg attattcaat
     7921 atcaatgaac caatcatttt cgggatgcca atcgtctaca atccctattt agcggttccg
     7981 tttttcttag ctccgatggc ttctgctaca ctcgcttatt ttgccattcg gctggaaatc
     8041 gtcaaaccga tgcttgcaca gatgccgtgg ccttcaccag tcgggatcgg agcatttgtt
     8101 ggtagtggtg gtgattggaa agctgctgtg ttagcagtca tttgtgcaat catcgcattc
     8161 attatctggt tcccatttat caaattctat gataataaat tattaaatga agaaagagaa
     8221 aaggcagcag aattagctag tgaaaagtta aaatgatttc taagaaaaaa ggaactgtga
     8281 caaaaaatca cagtcccttt ttttcttaga aatgggaaac cactagtata taaataccat
     8341 tattcttcaa tcaaccgctt cacgattgca taatcccctt cataaggttc atctacacca
     8401 ttgatttttt gatagggatc cacgcctttt cccgcaataa cgactgaatc ttgcggagcg
     8461 ctttctgcta aagcgcgtcg aatcgcttca ggacgatcca tttcataatg aacgatcaaa
     8521 tctggattag taattgcttc attgatttct tccgcgattt ttttcggatc ttcaaatgct
     8581 gggtcatctg ccgtcaaaaa ggcgatgtca gctgtttctg atagcacttt tccaaaatca
     8641 tgtcgacggg agattgcttt gtttcctggg ctgcctaata caacaattac acggccatct
     8701 ggatgttcat ttttggcaaa tttcagcaat gttttcaagc ttagataatt atgtgcatag
     8761 tccacataga catgcgctcc attggattgg atcaattgat ccattcggcc tggtacacga
     8821 gcttctttta atccgtcttg tgcatcttga ctggaggcac ctgttagtgc tgcggcaatg
     8881 atcgcactgg ctgcattttc ttggttgaat cctccagcca gcaaaatatc ataagcagta
     8941 tcgcagatag ctaagcgatc ttcatgagaa gataaagtaa aaccgtgagt acctttatct
     9001 gaacggatca cttgataatt gctttcttcc gaacgaccat aagtgatcgt tggaatctgg
     9061 aatagatcgg ctgtttccct aagtaaatca aaatagtcac tttcatgatt taaaacaact
     9121 gtttttgaat tcaagatcaa ttgtcgcttg caatagaaat agtcatcaaa tgtcggatgt
     9181 tcgatcggac tgatatggtc aggcgagata ttcaagaaga ttccgacatc taaagtcaag
     9241 ccgtatacgc gttgtgtttt gtaagcttgt gaagaaactt ccatcactag atgagtcata
     9301 ccattttcaa ctgcttctga catcatacga tataaatcta atgactcagg cgttgtcaaa
     9361 tgggatttga aatatgtttt gccatctaac gttgtattca tagtagaaag caatgctgtt
     9421 tttttatttg ttgtgtgatc caagattgct tttgtaaagt acgcagcagt tgttttacct
     9481 tttgttccag tgaatccgat cactttcaat ttattttgcg gatagtcgta aaaagccata
     9541 gcaagaagcg ccattgcttt acggatatcc gttacgatga ttcctactgc tgaattttcg
     9601 tatggttgtt ctgatacata atattgaata ccctgttcga ttgctgaatc caaatattct
     9661 tctttgaaat tcattccttt acagaaaaac aaagtagaag catcagcttt tcttgaatca
     9721 taacttaact gaagaaattc agctttttct ggtacatcaa gatgccagcc tcttgttgaa
     9781 acaaattctt ttaatagatt ttcttttaat agtaaatgac ggatgtcttc taaagagaag
     9841 ctcattgatt gatccctcgc tttattcaat ttcttggttt attatagcac actcttttta
     9901 atatatcgtt tgtttccgct atgacaaaat ccagctttat tcaaattttc ttgacacagg
     9961 ggacaagcgg atgagtattt tcataaacaa tgaactgact gtaatagata ggagaacgaa
    10021 tagtgtctcc aacaatccaa aaacataata attcaaaaag acgataagac tattggtaag
    10081 taaaagtcct tgcggcattg gaagccgaaa aaatttgtgg aaaataatcg gcggtatcgt
    10141 tataccgcca tttgaggccc cgactgtgta caatgtgtaa accccaagac tgaacaaaag
    10201 acttcctgaa atcagtgaaa agaaatgtga ggagatcatt tccacttttg gaacaatctc
    10261 taaaacaaca ggaaaaagca gacttcccgc tacagtctta aaaaatactg attttcctaa
    10321 taggacagcg gctagaagca gcatcataag attgatgctc caaacagtaa ctgagcgttg
    10381 gatagaaaag gccgtttcaa ctaaatgtcc gatggcgccc actccggcgg aagctatatc
    10441 gtgagggagc aagaaccaat tcaatccaac tgtgataaag aaaagaccta aaaatataac
    10501 gggtagtttt tttatttctt gtgtcattga tatctcctat aaatgttagc cataaaaggc
    10561 tgtatatttc tttactttac caaaaaaacg aaaccgaatt caaaaaaaca attgttctta
    10621 taaaagttat ataaaatcga ttgccagttt ctgttttacc aattaaaatg atagtaatgt
    10681 tttgtagaaa gttggaatct gaccgatgga taatcaaaga aaaccagcat tagatcaatt
    10741 aacaccgcaa gaaaaaatgg cacgaggctc tgcttggatg accgccagta atatcatttc
    10801 aagattgcta ggcgctgtct atatcattcc ctggtatgct tggatgggcg aaaatgctaa
    10861 ggctgccaat ggattattca atatgggata caatatatat gcattgtttc tactgatctc
    10921 tacagcagga atacctgctg cgattgcaaa acaaactgca cgctataatt cattgaatga
    10981 atatggcact tcacgacgat tatttatccg tgcattacag atgatgggcg ggttaggcct
    11041 gttgtttgct ttgttcatgt acatcgcctc tccttggcta gctcatgctt ctggtggcgg
    11101 agaagaactg attccaacga tgcgttcttt gagtatcgct gtattggttt ttccttgtat
    11161 gagtgtgata cgagggtatt tgcaaggcaa tcaagaaatg atgccttatg cgctgtcgca
    11221 gattgtagaa caggtggcac gtatttttta tatgctattg gctacattca tcatcatgaa
    11281 agtcatggat ggtgactatg tgacggcggt cacgcaatca actttcgcgg catttatcgg
    11341 tatgctagca agtattcttg tccttttgta ttttttgaag aaacatcaag cgtatacatc
    11401 ggcgtttatt cactacagcg aaaacaaaat aaatattgca acgaaggaat tgttgttaga
    11461 taccgttaaa gaagcgattc cgtttattat tgtgggttca ggggtaacga tcttcaaatt
    11521 agtcgatcag ttcactttta tgaagatcat gtctgcatcc accgagtatt ccaatgcaca
    11581 gttactggat cttttttcga tttttagtgc caatccagat aagttgacga tggtagttat
    11641 tgcattggca acatcgatcg cgtctactgg gttaccgttg attacagaag ctgttacgtt
    11701 gaaagatcga gtaggtctgg caaaattgac aagcggaaat ttacagttgt tttcattttt
    11761 tatgtttccg gcgacatttg gtgttatgtt acttgcctat ccgctaaata cattatttta
    11821 tacaccggat tctctaggaa gtaatgtact gatccaagca agttttgtag gactcttcct
    11881 aggactatat atgctagttt ccaacatgct gcaaggaatg tttgaaaaca aggcggcaat
    11941 taattatttg gttattgggt ttctagtgaa actccttctt caatacccag ccattcgttt
    12001 atttgaagta tacggccctt tattggcgac aatgatcgga tttgctgtgt cttgtacctt
    12061 gatcttaaag agaatccatg aagtagcccg ctttaaacca agttttgtct ggcgcaggac
    12121 attgctgatc tttattctta ccttgattat gcttttggct tcgtggatca caaagatgat
    12181 ttttggtgct tttttaaacg aagatagcaa attccagtct ctattgttga ttcttgtagt
    12241 agcaggtata ggggctttag tgtatggtta tttagctttg aaaatccgct tggcagaaag
    12301 actactagga ccaggaatag cgcgtttacg acataaatta aaaataaaat aagtgaatca
    12361 gaagagctag attgatgctc ttttgaaatg ggggaaacgc atgcgtttag ataagttttt
    12421 agcagatatg ggacttggca gccgcaaaga agtcaaagga ttaatcaaaa aaggaacgat
    12481 cgaagtcaat ggacaagtgg tgaaaagcga taaagcgcaa gtaaatgaat tcgaagatgc
    12541 ggttacttat ttaggagaaa tcattacgta tcaaaaagat ttttactaca ttttgcataa
    12601 acctgcagga gtcatttctg ctactcagga taatcatgat caaaccgtaa tcgatttatt
    12661 ggaagaccag gattatcggg aagatctttt tccagtcggc cgattagata aagacacaga
    12721 gggcctcttg attttaacga atgacggggt gtttgcccat cagttactat cacctaaaaa
    12781 acatgtggag aaagaatatc tagctgaggt tagcggtata atgacagaag aagatatccg
    12841 tttatttgca gaaggactga ttattgatgg cgatgagcag acattgccgg caaaactcca
    12901 aattgatcaa gtagatcaag agacagaaac ttcaattatc cgtctgattt tgcatgaagg
    12961 aaagtttcac caagttaagc gaatggtcaa agcagtagga aaagaagtct tatatttaaa
    13021 aagaatccgt atgggaaaat tttggctgcc taaagatttg aataaagggc aatatcgacc
    13081 catgaccaaa gaagagctag aacaagtgag agaaaaataa ttgaatggat agctaaaaga
    13141 ataagcagag tagttgtagc gccatctgaa aagcagactt gtccgtctga ctttcggatg
    13201 gcttttttgt gaaatttcgg cttatttccg tagaagctac ttctggaaca ccgtttgttc
    13261 ggaataaggg actgtgacaa gacttttgtt acagtcccca ttgttccttg tgactcagag
    13321 ttagatagta aggacacgat ctccctaata ctcaggccaa aacgctgaat tcacaacctc
    13381 tgataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga
    13441 ggaactattt tcccgttttt ttctctaata ctcaggccaa aacgctgatt tcacaacctc
    13501 tcctatactt tgtttcattt tccctatact ttgtttattt attctcatta ttgaaagcgc
    13561 tataattacg ttgcgaatga atcgaaaggg gtttttagat gaaaaaacga aaggggttca
    13621 atcatttttt tatgaatcta tggcgttata aagcacttgt cctgatggct gtaccgggta
    13681 tgctttggat gatttttttc ttttacatac ctgtaatggc gaatgtcgta gcattcaaaa
    13741 attttcatat ttcagcagat gggttcttgg ctagcttgag ggaaagtcct tgggtgggtt
    13801 gggaaaattt caaattttta ttttcttcgg atcaagcatt tctcatcact aaaaatacaa
    13861 ttttatataa tgtagcattt atccttttga atctgctgat ctcagtcgta tttgcaatca
    13921 ttatgagcga gttgagaaat aaacgactcg ttaaagttta tcaaacgatg tccttactac
    13981 cttattttct atcttgggtc attatcagtt actttgttta cgctttttta agtcctgaca
    14041 aaggaatatt caatcagtgg atcactggac atggaggaga agctgtcaat tggtataatg
    14101 aaccgaaata ttggccgttt atcttggtgt tcattggtac atggaaaggg attggttata
    14161 acagcattat ttactttgca tctgttatgg gaatcgatcc gacttattat gaagctgcta
    14221 tggttgatgg agctagcaaa tggcagcaga tcaaacatgt gacgatacca caacttattc
    14281 cactaatgac gattttaacg attttagccg taggaaacat tttccgagct gattttggcc
    14341 tgttttataa cattcctagg aattccggtg cattatatga tgtgactcaa gttttggata
    14401 cgtatatcta caatggcctg acatccacag gagattttgg tatgacggct gctgcaggac
    14461 tttaccaatc agttgtcggc tttgtcttgt tgatgatcac aaatacgatc gcccgacgtt
    14521 ttgatagcga ttctgcatta ttctaaggag gaaaaaaatg aaaaaaaaga aagtggagag
    14581 agtccaagtc cgctctttca ataagacggc taattttttc tctaatataa tgattgcact
    14641 ctttgctatt tcctgtattt tgccatttat atttgttatc gccatctctt ttaccgatga
    14701 gtccatattg tcgactcacg ggtacagttt ttggccagaa aagttcagta tatttggtta
    14761 tacgtacttg ttcgaccaga tgcaggataa aattttccaa gcgcttttcg tcactgtact
    14821 tgttactgtt ttaggtacac tgatcaatag cacggctact tctttatatg cttatgcgat
    14881 ttcacggtca aactttccat tccgtcgttt ttttaccgtt ttttgtttga ttacgatgtt
    14941 gttctcacca gggatggtgg ccaattatct tgttatgaca aatcttcttc agttaaaaga
    15001 tacgatttgg gcattgatct tgccaatggc tgtcagtccc ttcaatatta ttgtgatgcg
    15061 cacattcttt aaacgttcgg tctctgattc gattattgaa tcggcacgga tcgatggagc
    15121 tagcgaactg cgaatatttg ttcagatcgt tttaccgtta gctgttccag gtattgcgac
    15181 aatcagttta tttgccgcat tgggttactg gaatgactgg ttcaatgctt tactatacat
    15241 ccaagatgat aagctagttc cactacagta tcttttgatg aaaatccaaa gtaatattca
    15301 atatctgaca caaaattcag gagcaggaag ccaattgaca ggcggattag cctcagttcc
    15361 aggagaatct gccagaatgg cgatcgtagt catctcgacg ttgcctattg cgatcagcta
    15421 tccatttttc cagaagcatt ttgtcaaagg tctaacaatc ggcggagtaa aagaataaag
    15481 aaacaaaaat ataaatcata aaattaggag gaagaaaaat gaagttatgg aagaaactag
    15541 cctttacagg tgtttcagcg ttgttgttgg gaacattagc agcatgcgga ggttctggtt
    15601 ctaaagacca ggcagaagct agcaattcgg acactttgca gatgtatcaa atcggagata
    15661 aaccagataa tttcgatcaa ttgatggaag tagcaaacaa acgaatcgaa aaagagatcg
    15721 gtgtaaaagt caatatcaat tacattggct ggggcgatta cgaaaagaaa atgaacgtca
    15781 tcatttcttc cggggaaaat tatgatatcg cctttgcgaa caactatgtt tccaatgccc
    15841 aaaaaggagc ctttgctgat ttgactgaat tagcgcctaa gtacgctgaa aaagcttata
    15901 atgatttaga tgaagcatat attaaaggaa atctggtcaa tggaaaatta tatgctttcc
    15961 cagtcaatgg aaatgtcttt gctcaacaag tacttacttt taacaaagct ttactagata
    16021 aatacgatct ttcaattgat ggaatccaat cttacgctga tgcagaaaaa gtcttgaagg
    16081 aattccatga aaaagaacca aacacggcag cttttgccat tggacaaggc ttcaaagtcc
    16141 aagggaactt tgattatcct ttagggaata cgttaccatt tgctattgat ctggatggag
    16201 atgattcgaa aatcatcaac caatacgata atgaaacgta tatcggactg ttacgtacaa
    16261 tgcatagctg gtacaaacaa ggactcattc caagtgacgc tgcaacgagc aataaggatt
    16321 atccactaga aggaaatact tggctaatgc gtggagaaac acaaggaccg tatgattatg
    16381 gcgatacaat tttgacaaat gcagcaaaac aagaacttgt ctcaaaagca attactgttc
    16441 cactaaaatc tacagctcaa gcacaaatgg caaattttgt cgtatcaaat acttcaaaaa
    16501 ataaagaaaa atcagtagaa cttctagggc tgcttaacag tgatccagaa ttattgaatg
    16561 ggcttgtttg ggggatcgag ggagaagcat gggaaaaagt cgatggttca gatgataaaa
    16621 tcaaattgct agatggttat cagccaaata cacatatgtc tgcttggaat acaggaaaca
    16681 acaagattct atatacacaa gagtctatta cggatgacat gattgccaaa cgtgatcaat
    16741 cgattgctga tgcagaaact tcaccgattt taggattcag cttcaacact gacagtgtca
    16801 aaacagaatt aagtaatatt tctaatgtca tgaatcaata tttagatgga ttgaatacag
    16861 ggactgttga tccagatgaa acattaccga aattgaaaga cgctttggac aaggctggct
    16921 atgataaggt attgaaagaa atgcaaaaac aatatgatga gttccgacaa gaataataat
    16981 gaataaagac aaaggggttg tgacaaaaga cgtgtcacaa cctcttgttc cgaataaacg
    17041 gtgttcaaaa gccagtttct cggtattaag ctaaatatat gtaaaaatgt ggaaagccat
    17101 attcacaaaa tttttcttac tactcgaaac taagcggcta tttcacaaac tcttggtaaa
    17161 cggtgttcaa tcagctaacc ctcggtattc gctcacaaaa cgacaaaata tgaggaacta
    17221 tttttccgtt tgtttctcta ataatcaggt caaggtgctg attgcccaac ctttttttgt
    17281 ataatgagaa agtataaact taatgtttgg aggagaagat atgttaggga aggttttgga
    17341 agagttattt atccggatct gggtcattat aaaactaacc ttgtatttct ggatatatac
    17401 atttgccggc gggatcatct ttggattagg tgcggcatgg aaaacagtca atgaactatt
    17461 ttatttgtac ggattcgagt ataaagaaat cacaataaaa agaggctgga atatatataa
    17521 aagaaatttt ttaagaggca atctgctatt tagtttattt ttatctggta ctgctttatt
    17581 gagttacaat ttatatttat ctgttcaaat caaagggctc atttttttag ctattgattt
    17641 tattttactt tttgccttgc tctgcctatt tgctacttat caatacagta tgattttaga
    17701 tagtgaatat acgatctctg ttcctaatct gctaaagcta agttttattt cggttttttc
    17761 cagcttttcg tcttttctaa aaataattat tggtagcggt ataattttag gcgtgacttg
    17821 gcaattcaaa ggattgatcc tgtttggtgt cattggtctt ttgacggttt ggaatggtac
    17881 gatgacaaca cattggcgtg aagaattaga taaacagttg gaaagttatg aataagtttc
    17941 ggattctatt ttcaaaagga accttactaa atcgtctgtt gcggaaatac gcggttatca
    18001 tgatcggtgt aacgatgact gctaccgtta tttttagtat ccatacttgg gaacaaaatc
    18061 aaaaacaggc agaaaacatg acaagtgatg ccgttcaaag cacaagcaga atgctgaatg
    18121 ataaaacaac cttgagtagg atcatcaaaa accagcttgt agggaattcc gaaaagatcg
    18181 aaaatgtgac gacctactta acaaaaccca tagatcagta tctgatgtat gtatacgaac
    18241 agcaaaacag taccgatgaa ttagtttctt tcccaaatca aatcaaagat ctctacgcaa
    18301 attatgaaga actaagtgcg atatatatcg tgttgaatca actaccggag tattatgaat
    18361 cttctagaga aaacaaaggt ggggaaataa aaaaggggac accaaaatta ctagacgcct
    18421 tttatatcaa gttgcctatc acacaaggag gagctgagtc aggaagtatt tttattggtt
    18481 ttcaaaagaa agaactagat atgattttag aaaatctaac gtcattcaat ggactctctt
    18541 tatacatgat ttctggaacg acaaatcgac tatacacatt ccatgataat aaaatagcta
    18601 aagaaacgtt tgaaagacaa gaaatgatca tcacagagag tctgaagaaa aattcacaat
    18661 tgccgattga cgatctggaa aaaaacaatt ttgttcaaca ccaagaactg tctgatgatt
    18721 atcgtatatt agctgtacta gataaaaaaa gtgtaaaaat cgaaacggga aaaaatcttg
    18781 ccccgctgat cattggtatc attattttag acaatatact gcttatcttt ctttataaaa
    18841 cattcaaacg atattcacag caagtatcta tcgtcatgca ggccatggat caaacagcag
    18901 cagggaattt agagacgcgg atcgacataa caaacacgga atatgaactg aaggaacttt
    18961 caatcggtat caacgaaatg ttggatagca tcaatcagtt tgtagaagac atctacaaat
    19021 tagaaatcaa acagcaagac gcacatatgc gggcgcttca agcacagatc aatccacact
    19081 ttttatataa tacattagaa tatatccgaa tgtatgcaat cagcgaagga agtgaagaat
    19141 tagcagatgt tgtgtatgcg ttttccgctt tgcttcgaaa caatacgaat caagagaaaa
    19201 cgatcacttt aaaagaagaa ctggactttt gcgaaaaata tgtctatttg tatcaaatgc
    19261 gttatcctaa tcgagtagcc tatcatttta tgatcgatcc tgatttggaa aagatcgaag
    19321 tacctaaatt tgtgatccag ccattagttg aaaattattt taaacacggt attgatttta
    19381 ctcgattcga taatgcgcta agtgtcaaag tcttgcagga aggcaaacgg gtaaggatca
    19441 tcatcaaaga taatggaaaa gggatgactg aaaaacggct aaaacagatt gaagaaaaac
    19501 tgtctcatcc taaagtggaa cttcacggtt ctatcggtct gcaaaacgtg aatgaacgtt
    19561 tacgtgccag cttcggcagc agttattata tgtcgcttga gaataatgag acaggtggac
    19621 tgaccgtttc tattacattc aaggagggat gagcgtgtat caagtgttat tggtagatga
    19681 tgaatacatg attttgaatg ggttaaaaaa gataattgat tggcaaagtt taggctttca
    19741 aatagtagca acagcagaga atgccaaaga aggactagct gtacttgaac agcgacagat
    19801 tgatttagta gtaacggatg tgacaatgcc agaaataaat ggactggaat ttattgaagc
    19861 ggctcaaaaa gaacgtcata attttgaatt tatgatttta tcaggatacc aagaattcga
    19921 ttatttgaaa ggaggtatgc aattaggcgc cgtgaattat ttgatgaaac ccgtgaataa
    19981 gttcgaattg gttgaaagcc tcaagaaaat caaaacacgg ttagatcagc aaaatgaaca
    20041 aaaaaatcaa caagaaatct atcaagaact gttattcagt caatggttga atgaagaatt
    20101 ggatgaaata agtgaagaag aaataatagg acaaataagt gagaagcagc ataggatagt
    20161 attgattcaa ctggcaagaa ctcatgatac tcttatcaat agatggttgc aagaacggca
    20221 acaaatcttt tactatcaac gaaattacgg agaccgtgta ttgatcacgt tgttgttgga
    20281 ggcagatgaa gttgatggct tctgttgttt tgttcaagaa aactttcctg atcaggaatg
    20341 gctgatcagt atcggggaag agacagattc tttggacaaa atccctgaaa cattccaaca
    20401 agcgaaagac aatctacagt tgcatcaatt ttatggagat aaagacgagc atgtttttta
    20461 cgcagtacaa gcagaaacaa aagatcaaac catcgatttt tcaagtttca gacgcgtgct
    20521 gcaaaataaa caattagaag tcgctcaaaa aatgatcact gatttttttg aacagtttca
    20581 gttagcagcc atgatgccgg aagatatacg atattccagt tttttactgt ttatggagat
    20641 tcaacgggaa ttgattgatc tggaagatga ggaatattta caaggaatcg aaaaaatcca
    20701 tcaggcaaaa acggtgcaag aacttcagca gctacttctg tcattcatca aaaagcatca
    20761 gagacagaaa aagtacagtg acaatgtgga aaaagtaatc gtaatcctgc accagcatta
    20821 tcaagaacca cttacactca aagaagtgtc agaaaacctg catttaaatg tcatgtatct
    20881 aggacagctg ttcaaaaaag aaacgaaaaa aagtttttca gcttatttga accatttacg
    20941 tatggaaaaa gcgaaacaac tgcttttgca cagtaatcaa aacatcaatg agatagcaag
    21001 tgagattggc tacaacaaca ccacgtattt ctctaaatta ttcaagaaaa tcgtcggcag
    21061 atcccctaaa gagtaccgag aaaacagtgg agaatcataa aagaaaaagc caatgtaatg
    21121 aaataactta taaaaaattg tctagaaatc taattttggg ctgttcttat tacttgaggc
    21181 aaggcagctt tttcatgacc tctcgataaa cggagttcaa aagccagttt ctcggtatta
    21241 agccaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt aatactcgaa
    21301 actaaacagc tttttcacga cctctccact aaacggtgtt caatcagctg accctcggta
    21361 ttagctcaaa aaatggcaaa atatgaggaa ctattttgcc atttgtctct ctaatactca
    21421 ggtcagaacg ctgatttcac aacctctcaa taaacggtgt tcaaaagctt gcatctgcgg
    21481 taataagacc aaccaaacga aaaatatgag gatctatttt tgcttggctg ttcttattac
    21541 ttgatgcagg acagcttttt cacaacctct tgttaattat agtttgataa ggaaaaaggc
    21601 aaatcttttg cttgatattt cttagggtga gggatgatcc ctaattggta agagatcgtg
    21661 ccgcctttca gcaaatcttg atgggtaaaa tataatttcg tatattcttt gtccatccgt
    21721 ttgattttat ggataaattg ttgctgcggc cgattaggcg ttgcttcaat cgtcaattcc
    21781 tttccatttg acaaaagcaa aacagccttg tcaattaatg gcataccgat aacatattca
    21841 ccggttccag gcgtgacagg ataaaatccc agactattga agatatacca agcggccata
    21901 cttccattgt cctcatctcc agggtaaccc attggagaat cgtcgaaggt ctgagtcatc
    21961 aactgtttaa gcagtggctg tgccatttca ggttgtccta tataactgaa caagtaagga
    22021 tagtgaaaac ttggctggtt tgaaatagct aattgtccaa attctatcgc ggccatctca
    22081 ctcatttcgt ggatctcaaa accatatcct cctgggtcaa acgttggggg ctgattgcac
    22141 agctcaatga gtttttcttt atatctctca ctgccaccat attttccgat cagtcctgaa
    22201 aaatcgtgat aaacagcaaa gctgctttgc caagcacttc cttctgcata atcttgtccc
    22261 caacgattag ctgaaaatgg ttctctgaac ttgccatctt tatcttttga acgcataaat
    22321 cctgactcat agtcaaagat attttgatag ttcaatgatt gctgctgata tttcttcgtc
    22381 atttcctctt cttctagtac cttggcaact tggctaatac aaaaatcgct ataagcatag
    22441 tctaatgtgt gattgactga ttcgtggtag tgattaggaa catagccata tgtcaagtaa
    22501 tcttctgttc cttggcgtcc ataatttgga tgtttgcttt gaaccgtagc acctttttcc
    22561 attgccgtta gaaattcagg catcaagtct cgtcggatat cttttacagc agcatctgca
    22621 atgaccgcat cgatcaatgt cccaggcata agtccacgtt catcaggaga cagccatttt
    22681 ggcaaatagc cactttctct atagctgttc aaaaaacctt ccagcatttc ttcgtatttt
    22741 tctacagcaa tcaaagaata aagaggataa accgttttat acgtatccca aaacccatta
    22801 tttgtataga gaacaccagg ttttatagat cgactagtcg tatcatagtg gattttttgc
    22861 ccactaggat caatttcata aaatgtttgt ggaaataaaa acacccgata taaattatga
    22921 taaaacgtcc gcaattgttg ttgatcatgg tgtgtgagct gtatacgatc aaaataggac
    22981 tgccattgtt cttgactgtg agcaaggtaa gtctctttgc tttggttttg ttctagttcc
    23041 aggttataag ctgcttgttc cgagctgata aaagatgtcc caaatcggat agtttgtttt
    23101 tttgtctgtt caaaatacaa cgggatcgag tcgctatttt tgttggaaac agctaactca
    23161 tcaattgtta aaggatattc aaatctcaat ataaaataaa aagaaaaatc aggatcttct
    23221 gaaccagcaa aatgtgtgat ttttccttcc actgtgtaag gatctataac aaagagctga
    23281 tggcgtcccg gaaaggataa aagtaaaccg ctgtcatttt tgctgtaatc aatagataag
    23341 attccgccat acatgctagg tatcaatgta gaacagatgc cataacgtaa ttgacagatc
    23401 gacagatgag tcgggcaaaa tatactttct tctgggcggt aagaactttg agcatggaaa
    23461 agggtgttct cctgtagttt tccattgatc ggcgtaaaca gcatatgact gaaatcaccc
    23521 atccaaggac ttggttgatg ggtaagacgg tagccttgaa aaacgcggtc atcaggatga
    23581 aaccaccagc tgccgttttg atccgtagtt tgcggtgcaa aataattcat cccaaaagga
    23641 acgccagtat aaggctggca gttcccatta gagaaatttg gctgattcgc tgttgcatgt
    23701 ctagtatcta tttgaccgat attcatttta tttctccttt gttgattgtc ttttgatgaa
    23761 tgaaactgga aatttactat cggatggtcg atcttttgtc tctaaccaag tgaccaaagc
    23821 atctcccgca agcttaccta actgttcaaa atcttgtgcg atggttgtca gtggcgggtc
    23881 aacaagagct gctgcttgga tatcatcaaa tccgataatt gaaaaatctt cagggatatg
    23941 ataaccatgc tgtctcaaga gattcatggt ctggatagcg acaacatcgt tttcacaaac
    24001 aaaagcagtt acttcatact tagtgatata agaaagcaat tcatctaatg tcgcgctttt
    24061 gtcatccagt gaagtgtgga aagtcaaacc agctttgtcc aatgcttcaa ggtaaccaag
    24121 gtaacgctgt cgaacggatt gtggatgagt ctgcgtacca aacaaataag cgatttttga
    24181 atgtccctgt tcgatcaaat aagaggcagc aagcgcaccg ccttggaaat tatcggaaag
    24241 aacggtagga aaaggtaact catagatttt tttatctaat atgacgacag ggaattcttt
    24301 caatgaaagc tcaaaaagta agtcaagatg ttgttctgta tcaagagcat aataaatcaa
    24361 tccgtcaaat tcattgatta gctcttttgc tgttttattt tgtagaaagt caagcgtcgt
    24421 catcatgaca tcaatctgtt tatcttgcat cacagggttc aatcccttgc taaaatcacc
    24481 aacagacaaa tcagcgacat atggaagaag aaacagaatc cgtcgtgttt ttgtaggaga
    24541 agcaagaatc tgttccggag aagcggattt aacgaaactg cctttcccgc gaacgcgata
    24601 aataagtcct aattgctcta attcagtcaa tgcacgtttg gaagtgattc tgcttacttt
    24661 ataggtacgt gaaagttctt tttcagttgg tagttggctg tctacaggta attcaccagt
    24721 taaaatagcc gtttgcaaat cattaaatat tgtctggtac aatggttgat tcatataaac
    24781 acctcttaat gatatatcat attcaaataa taccataatg gatgaaaaag taaataaaaa
    24841 gatatttttt gaaagcgtag acataattgg ctgtatgaag tataatcttt aaagatatat
    24901 caaatagaaa gaaggtttta ttagtggctt ataaagatat accaaaatcg gtgcagaaat
    24961 ttatggaaga tatcacacaa aaatgtgggg atatccatgc ggactgggca aagaatttta
    25021 cagcagcatt tgccaatact ctgttgacaa cagtcaaaaa gcaaaatgac ggaaccacct
    25081 ttttattgac gggagacatt ccagcgatgt ggttaagaga ctctactgca caagttcgtc
    25141 cttatttagt gattgcaaaa gaagatgagg aattagctga catgatcagc gggttagtca
    25201 aaaagcaatt tttttatatc aatatcgatc catatgcgaa tgccttcaac gaagaagcta
    25261 acggtgctgg ccaccaaaca gatcatacaa aaatgaacga ttggatctgg gaaagaaaat
    25321 atgaaattga ttctttatgc tacccaatac agttggccta tcttctttac aaaaatacag
    25381 gaaaaactga tcaattcaat caatctttta ttgaaggtat caaaaaaatc ttggacgtgt
    25441 tcaaaacaga gcaagatcat gaacactctc cttatacatt tgtacgagac acgacaagaa
    25501 aagaagatac gctgatcaat gacgggcgag gtgcaaaaac agcttttaca ggaatgacat
    25561 ggtctggttt ccgtcctagc gatgatactt gccgttatgg atatctggta ccatccaata
    25621 tgtttgcggt ggtcgtgtta ggttatgtgg aagaaatttt tcaaacgata ctcgatgata
    25681 aagaaattgt acagcaaaca cagaaattga agaaagagat ccaagagggg attcagaatc
    25741 acggacgaac ggttaatcaa aaagggcaaa cgatttatgc atatgaagta gatggaatgg
    25801 gaaacgccac agtcatggat gacagtaatg tgcccaatct gatcgccgca ccttacttgg
    25861 gatattgttc tgaacaagag gaagattatc tgcaaacaag aagaacattg ttgagtaaag
    25921 aaaatccata tttctatgaa ggaaagtacg caaaaggaat tggatcctcc catacgccag
    25981 aaaaatacgt ttggccgatt gcattagcta tggaaggaat gacaacgtca gacaaatctg
    26041 aaaaagagcg gatattgaat ttacttgtag caactgacgc aggaacacac cttatgcacg
    26101 aaggattcga tgtgaacaat cctgaaaatt atacgagaga atggttttct tgggcaaata
    26161 tgatgttttg cgaattagtc atggattact ttgatattag agtagaaaag taggaggtat
    26221 cgtaaatgaa aaagaaagta tatatcattt ctcatagcca ctgggatcgt gaatggtaca
    26281 tggcttacga acagcatcac atgcgactga ttgaattgat agacgatcta ctagagctat
    26341 ttgaagttga tccgtccttc aacagttttc atttggatgg gcaaacgatt attttagatg
    26401 actatcttca agtaaggcca gaaaaaagac aggcaatcca acaagcaatc aatgaaggaa
    26461 aattgagaat tggaccattt tatatcttgc aagatgattt tcttatcagc tcggaatcga
    26521 acgtgagaaa tatgctgatt ggcatggaag aaagccgtaa atggggaacc cctgtcatgt
    26581 taggctattt tcccgataca tttggaaata tgggacagac gcctcaatta atgaaacaag
    26641 ctggtatttc agcagcagcg tttggccgtg gtgtaaaacc aattggcttt gataatcaag
    26701 ttttggaagc agaaaactat tcttcccaat actcagagat gtggtggaaa ggacctgatc
    26761 aaacagcaat ctttggtctg ctatttgcca attggtatag caacgggaat gagattccag
    26821 tagaaaaaga agcagctctt gccttttgga agcaaaaatt agcggatgca gaacaatatg
    26881 cctctacgaa ccacttattg atgatgaatg gtgttgatca tcaaccggta caaaaagata
    26941 tatcaaaagc aatccatctc gcaaatgaac tgtttccaga ttacgagttt attcattcta
    27001 attttactga ctatctggaa gcagttcaaa aagatgtgcc agaagactta ggctcagtag
    27061 aaggagaatt gacaagtcaa gaaacggatg gctggtatac tttagccaat acagcatctg
    27121 ctcgggttta cttaaaacaa tggaatacca aggtccagcg acagctagaa aatattacag
    27181 aaccacttgc gacgatggct tatgaagtta gtggaaacta tccccatgat caattagatt
    27241 atgcttggaa gacattaatg caaaatcatc ctcacgacag tatttgcggt tgttcagtcg
    27301 attctgtcca tcgggaaatg atacctcgtt ttgaaaaagc agatgaagtt ggaaagtatc
    27361 ttgcacaaga ttccttagaa cagctgaccg cagcaatcga tacgaccgga ttcccaaaag
    27421 acagctttcc atttgtcatt gtaaatactg caggaatgga taaaactggt gaagcagaaa
    27481 taaccatcga attagaaaga aaaagattcg ctgaaggaat acctgaacaa ctatatcaag
    27541 agttagaaaa tttgcctaaa cgtaagtatc atgtagaaac aaaaagtggg gccactatac
    27601 cagctatttt atctgaagaa actgtccaat ttggttatga tttgccgaaa gaccgtttcc
    27661 gtgtgccata tatggcaaga atgatcaaag tgactttacc attagaaaac atgccagcat
    27721 tttcttggga aacattcgcg cttgtagaag gggaagcgaa agcagaagaa aaggaaacaa
    27781 tgattcatca gagtggacga atcatcgaaa acggccctct acatttgacg attgaaaaaa
    27841 atggtaccat tacaatggaa gatcgcaaaa acaaacgaaa gttgaataat ctgcatattt
    27901 ttgaagatat cggcgatata ggaaatgagt atatctttaa acaacctttc tgtgatcagc
    27961 caatcctatc cagtaacaaa gaaaactctg aagtaaaagt attagtagat acaccggaaa
    28021 tagcaaaaat aagtattcta caggaaatgg aaatccctgt ttctgcagac gagcgtttag
    28081 aaaaagaaca gcagatggtt gtggaatttc gttatcgaaa agcagaacga tccaaagaaa
    28141 aacgaatcct tcaaataaaa acaatcatga caatcagaaa agatagtaaa aaaatcgact
    28201 ttgaaacgac tctcgacaat cagatgaaag atcatcgctt acgtgtcttg tttcctacaa
    28261 agttacatgt ggaacatcac gaagcagata gtatttttga agtggtgaag cgcccaaacc
    28321 atgtgtctaa aagttgggaa aaccccacga atccgcaaca tcagcaagca tttgtcaata
    28381 ttcatgatga agagtacggt gtaacagtcg gaaactttgg tttgaatgaa tatgaagtaa
    28441 cagaagacgg acaaatcgca gtgacgctac ttcgcagtgt gggagaacta ggcgattggg
    28501 gatattttcc aacaccagaa gcacagtgtc taggtgagca tcggttcaat tacagcattg
    28561 aactacatgg accagaagaa aaattctcta cttatttgca tgcatacgca gcacagattc
    28621 ctttttccac tcaacaaatc aaacatcatg aaggaacatt gatttcaaaa caacaatatt
    28681 taacgataaa aagtgaaacg tttgcaatta cagctttgaa acgcagcaag tttagtgata
    28741 aagtagtagt aagaggtttc aatatgtcta gtcatttaga aaaattggaa ataactaaag
    28801 acaatggaaa aactgtcata ttgaatctgc ttgaagaacc aacgaagcaa gcggttgtcc
    28861 caataatcca accttatgag attcggacaa tcggattcga ggaggaaaac gaatgtatgg
    28921 cggaatagaa gcaggcggaa caaaatttgt gtgtgcggtt tcagatggtg gagaaatttt
    28981 aga
//
LOCUS       DAYYBG010000023        28603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000023 DAYYBG010000000
VERSION     DAYYBG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.023"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACHAUN_001065"
     CDS             237..1877
                     /locus_tag="ACHAUN_001065"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8459783.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACHAUN_001066"
     CDS             2031..3353
                     /locus_tag="ACHAUN_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8459784.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACHAUN_001067"
     CDS             complement(3415..3846)
                     /locus_tag="ACHAUN_001067"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8459785.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACHAUN_001068"
     CDS             complement(3910..4746)
                     /locus_tag="ACHAUN_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8459786.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACHAUN_001069"
     CDS             complement(4936..5238)
                     /locus_tag="ACHAUN_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8459787.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACHAUN_001070"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACHAUN_001070"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8459788.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACHAUN_001071"
     CDS             complement(5692..5958)
                     /locus_tag="ACHAUN_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8459789.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACHAUN_001072"
     CDS             complement(6212..6937)
                     /locus_tag="ACHAUN_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8459790.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACHAUN_001073"
     tRNA            complement(7303..7373)
                     /locus_tag="ACHAUN_001073"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACHAUN_001074"
     CDS             7490..8242
                     /locus_tag="ACHAUN_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8459791.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACHAUN_001075"
     CDS             8258..8737
                     /locus_tag="ACHAUN_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8459792.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACHAUN_001076"
     CDS             complement(8988..10568)
                     /locus_tag="ACHAUN_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8459793.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACHAUN_001077"
     CDS             complement(10561..11304)
                     /locus_tag="ACHAUN_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459794.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="ACHAUN_001078"
     CDS             complement(11480..12256)
                     /locus_tag="ACHAUN_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8459795.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="ACHAUN_001079"
     CDS             complement(12504..13340)
                     /locus_tag="ACHAUN_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8459796.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="ACHAUN_001080"
     CDS             complement(13351..14280)
                     /locus_tag="ACHAUN_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8459797.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="ACHAUN_001081"
     CDS             14472..14825
                     /locus_tag="ACHAUN_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8459798.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="ACHAUN_001082"
     CDS             complement(14899..15555)
                     /locus_tag="ACHAUN_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8459799.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="ACHAUN_001083"
     CDS             15781..17715
                     /locus_tag="ACHAUN_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8459800.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="ACHAUN_001084"
     CDS             complement(17801..19024)
                     /locus_tag="ACHAUN_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8459801.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20889)
                     /locus_tag="ACHAUN_001085"
     CDS             complement(19018..20889)
                     /locus_tag="ACHAUN_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8459802.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="ACHAUN_001086"
     CDS             complement(20910..22028)
                     /locus_tag="ACHAUN_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8459803.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="ACHAUN_001087"
     CDS             complement(22111..22944)
                     /locus_tag="ACHAUN_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459804.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="ACHAUN_001088"
     CDS             complement(22937..23854)
                     /locus_tag="ACHAUN_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459805.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="ACHAUN_001089"
     CDS             complement(24081..25205)
                     /locus_tag="ACHAUN_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8459806.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="ACHAUN_001090"
     CDS             25388..26296
                     /locus_tag="ACHAUN_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8459807.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="ACHAUN_001091"
     CDS             26437..28050
                     /locus_tag="ACHAUN_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8459808.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5622 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta aca
//
LOCUS       DAYYBG010000024        27605 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000024 DAYYBG010000000
VERSION     DAYYBG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.024"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..1941
                     /locus_tag="ACHAUN_001092"
     CDS             175..1941
                     /locus_tag="ACHAUN_001092"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8459809.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2043..3302
                     /locus_tag="ACHAUN_001093"
     CDS             2043..3302
                     /locus_tag="ACHAUN_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8459810.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3449..4750
                     /locus_tag="ACHAUN_001094"
     CDS             3449..4750
                     /locus_tag="ACHAUN_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459811.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4753..5604
                     /locus_tag="ACHAUN_001095"
     CDS             4753..5604
                     /locus_tag="ACHAUN_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8459812.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5617..6432
                     /locus_tag="ACHAUN_001096"
     CDS             5617..6432
                     /locus_tag="ACHAUN_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8459813.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6583..7749
                     /locus_tag="ACHAUN_001097"
     CDS             6583..7749
                     /locus_tag="ACHAUN_001097"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8459814.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7979..8626
                     /locus_tag="ACHAUN_001098"
     CDS             7979..8626
                     /locus_tag="ACHAUN_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8459815.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8710..9654
                     /locus_tag="ACHAUN_001099"
     CDS             8710..9654
                     /locus_tag="ACHAUN_001099"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8459816.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9789..11240
                     /locus_tag="ACHAUN_001100"
     CDS             9789..11240
                     /locus_tag="ACHAUN_001100"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8459817.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11261..12097
                     /locus_tag="ACHAUN_001101"
     CDS             11261..12097
                     /locus_tag="ACHAUN_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8459818.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12097..12756
                     /locus_tag="ACHAUN_001102"
     CDS             12097..12756
                     /locus_tag="ACHAUN_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8459819.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12882..13571
                     /locus_tag="ACHAUN_001103"
     CDS             12882..13571
                     /locus_tag="ACHAUN_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459820.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13577..14533
                     /locus_tag="ACHAUN_001104"
     CDS             13577..14533
                     /locus_tag="ACHAUN_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8459821.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14577..15089
                     /locus_tag="ACHAUN_001105"
     CDS             14577..15089
                     /locus_tag="ACHAUN_001105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8459822.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15345..15893)
                     /locus_tag="ACHAUN_001106"
     CDS             complement(15345..15893)
                     /locus_tag="ACHAUN_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8459823.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15982..17163)
                     /locus_tag="ACHAUN_001107"
     CDS             complement(15982..17163)
                     /locus_tag="ACHAUN_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8459824.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17399..18907)
                     /locus_tag="ACHAUN_001108"
     CDS             complement(17399..18907)
                     /locus_tag="ACHAUN_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8459825.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19066..19257
                     /locus_tag="ACHAUN_001109"
     CDS             19066..19257
                     /locus_tag="ACHAUN_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459826.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19460..20377
                     /locus_tag="ACHAUN_001110"
     CDS             19460..20377
                     /locus_tag="ACHAUN_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8459827.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20370..21443
                     /locus_tag="ACHAUN_001111"
     CDS             20370..21443
                     /locus_tag="ACHAUN_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459828.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21591..22109
                     /locus_tag="ACHAUN_001112"
     CDS             21591..22109
                     /locus_tag="ACHAUN_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459829.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22090..22482
                     /locus_tag="ACHAUN_001113"
     CDS             22090..22482
                     /locus_tag="ACHAUN_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8459830.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22558..23493
                     /locus_tag="ACHAUN_001114"
     CDS             22558..23493
                     /locus_tag="ACHAUN_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8459831.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23626..24405
                     /locus_tag="ACHAUN_001115"
     CDS             23626..24405
                     /locus_tag="ACHAUN_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8459832.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24398..24715
                     /locus_tag="ACHAUN_001116"
     CDS             24398..24715
                     /locus_tag="ACHAUN_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8459833.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24959..26569
                     /locus_tag="ACHAUN_001117"
     CDS             24959..26569
                     /locus_tag="ACHAUN_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8459834.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26726..27574
                     /locus_tag="ACHAUN_001118"
     CDS             26726..27574
                     /locus_tag="ACHAUN_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8459835.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8932 a         4884 c         5544 g         8245 t
ORIGIN      
        1 gtacaggaag tagattaaaa cttttaaaag ttttaagtct atctatttgg caagtttaaa
       61 aaataaaaat tcggtatcaa aaaactgtta ccgataacat ttttgaaagc gttgtctttt
      121 gtttctctat cacttatagt gatgacgtca tattaaaaca caaggaggaa atcaatggat
      181 acagctgcaa tttatcatcg accggaaagt gaatatgcat ttctatatac gaatacaaga
      241 tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt gctttgtgga
      301 gatccatata ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt
      361 ctaagtacca atgttcatga ttattgggaa attgaagtga cgagcgaaac aaggcgctta
      421 cagtatgcgt tccatgttgt tggggaagat aacacggact gtttttacgg tgatcaagga
      481 atttttcctt atcgagaaaa tgtgattact gaaccaaatt tttattttcg aataccttat
      541 tttcatcaaa tcgatcgatt cactattcca gaatgggtaa aagaaacagt atggtatcaa
      601 atttttcccg aaagatttgc aaacggggac aaagcaaatg accccgaaca cactttacct
      661 tggggaagta aagatccaga ccgagaagac ttcttcgggg gagatttgca aggtgtgagg
      721 gatcatttgg attacttgac ggaccttggc gtcaatggca tctatttttg tcccatattt
      781 aaagccacat ccaatcataa atacgatacg attgattatt atgaggtgga tcccgctttt
      841 ggcgataaaa aacttttaaa aaacttgatt gatgaagcac ataaaagagg gatacgtatc
      901 atgttcgatg ctgtattcaa tcatatgggt gtccattcac cacagtggca agacgttctc
      961 aaaaacggag aaaaatcgat ctataaagac tggttccaca tccgtttttt cccagtagat
     1021 tcttatcaga tgactgactt accagaaact gctgaaaata tcccatatga tacttttgct
     1081 tttactccat tcatgccgaa actaaataca gcaaatccag aagtgcaaaa atatttgctg
     1141 gatatcgcaa cttattggat aaaagaattc gatattgatg gatggcgtct tgatgttgct
     1201 aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag cagttacaga aatcaaacca
     1261 gacatctata tccttggaga gatttggcat tcctctcaag cttggctgca aggggatgaa
     1321 ttccatgcag tgatgaatta tgcattcaca gattcgatta aagattattt tgcaaaaaag
     1381 aaaatcacag cttcacaaat ggtcagtgga atgaatcatc agcaaatgct ttacagagat
     1441 caggtgaacg aaggaacatt caacttatta gattctcatg atactgccag gatcttgacc
     1501 ctttgccaag gtaacaaaga attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa
     1561 gggtctcctt gtatctatta cgggacagaa atagggatga ctggtgaaga tgacccggat
     1621 tgccgcaaat gtatgatatg ggagaaggag gaacaagatc tagagctatt cggttttata
     1681 aaagaacttg tttctctaag gaaacagttg tctaagatta tttctgaagg ttctactcag
     1741 tggctaatag tatatgatcg tgaagataag ctctatttca cacgggaact agagggacag
     1801 atcatctatg tttactttaa tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag
     1861 gtgattctta gtcaaaattg tcagttgtta gaagacggga aagcagagat acgacaatac
     1921 ggatttttga tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg
     1981 aagatttgtg cccattataa taagggtgta acagcacgtt gaatacacag gaggaaatga
     2041 agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt aatctcttta ggaatcttag
     2101 gcggattagc ggcttgtggc aatggtaact cttcagcaga aggggaagcc gatgaaggaa
     2161 aaacgttaac agtttccgtt gattctggat ataaagatta tataaacgaa ataaaaaaca
     2221 ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga aaaggacatg ttcgatcagt
     2281 tggaatcatt agcgttagat gggcctgctg gaaagggacc tgacgttatg atggcagcat
     2341 atgaccggat cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg
     2401 aagcagctta cgatgaaaca gacaaagccc aagtgactta tgatggaaaa atctacggag
     2461 aacctgcagt aatcgagacc cttgttttgt actataacaa agatttagtc gatacagcac
     2521 catcaacgtt taaagatttg gagaatctat ccaaagacag tcgttttgct tttgagtcag
     2581 aagcaggaaa aaatacagga tttttagcta aatggactga cttttattat tcctatggtt
     2641 taattgcagg ttacggtggc tatgtatttg gtgatgatgg cacaaatcct tcagatatcg
     2701 gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg
     2761 tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc cagcaattca
     2821 tgtccaataa aactgcagca atcattgacg gtccatggca agcgcaaaca tacaaagaaa
     2881 acaatatcaa ttatggtgta gccaaaattc ctactctaaa taatggacag ccttatcaac
     2941 cgtttggcgg aggaaaagga tgggtagtca gcaattatgc taaaaacaaa gacctctctc
     3001 aaaaatggct tgattatgta acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa
     3061 atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac gatgaattaa
     3121 caacagctgt gattgaacaa tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg
     3181 gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac
     3241 caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga agccatagaa caaaaatact
     3301 gattaaaatg ataaccgtcc atttttataa aaaatggaac aggtgcttca gataaaagtg
     3361 gcaagagcga gatggaaaat tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg
     3421 gtttagcaag tttagagggg gcattcagat gcagcaagat aaaacaagca atatcaacga
     3481 aaaaaaaatc acgctttgtt cacttattcc agggatgggg cagtttgtaa atggccaagc
     3541 ttttaaaggt atcgtttttt tcgccatatt cgtcctattc attggtcaga tgcttctggg
     3601 cggatttcat gcattggtag gattggttac tttgggaagt ataccaatgg aagatcattc
     3661 attatttatt ttgattgaag gcactttgca attcatcatt acaatcattt tcttgatttt
     3721 ttacggtttg aatcttttag acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa
     3781 aatcaatcgg acgatgaagg aagtcataca taatataggt gatgaaggtt ttccttatct
     3841 attgacactt cccgcttatc ttctcatgat ttttacgatc attttcccag tcctagttac
     3901 attgttcact gcatttacta attatgactt caatcatatt ccaccagcca gtttaattga
     3961 ttgggtaggg gctaaaaact tttttagtat cttcttttta agctcatatc gtaatacttt
     4021 ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt gcaacaactt tgcaaatcac
     4081 ccttggtatt ctcacagcgg ttgttgctca ccagaaattt attaaattca aacgtatttt
     4141 cggtatcatt ttcttattac catgggcggt cccagctttt attactatca tgagtttttc
     4201 gaatatattc aacgatagcg taggtgccat caatactcaa gtgattcctt tcataaacca
     4261 gcttcctttt gtagatatca gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt
     4321 agcaattatc ttagtgcagg gctggttagg atttccctat atctatgtga tggttacagg
     4381 aatcttacaa tcgatttcag aagacttata tgaagcagca aaaatcgacg gagcaaatgc
     4441 atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt
     4501 tattacacag tatacgggga atttcaataa tttctccatg atctatctgt ttaacaatgg
     4561 tggtcccgga agcgtaggcg gaggagctgg ttctactgat attcttattt cctggatcta
     4621 taaacttacg actggaactt ctccgcagta ttctatggct tcagcaatca cgttgattat
     4681 ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa aagacaaatg cgttcaatat
     4741 gggggattaa caatgaaaaa atttcattcg agtgctcgat tccaaaaacg actttctcac
     4801 ttttttactt acttttttct tttaatactg tcagtcatta tcatctatcc gcttttgatc
     4861 acagcaagta ctgccttcaa gtcaggaaat attgcagctt ttagtttaga tctttcaagt
     4921 gactggactt taggaaactt tactcggcta ttcagtgaaa cactttacgg gacttggtat
     4981 aaaaatacat tgatcatttc tgtcctaaca atgatcgtac aagtgactgt agtaactttg
     5041 gcgggctata cttatagtcg ctatcgtttt atcggccgca aatcaagttt gatgttcttt
     5101 ttagtgattc agatggtacc gactatggca gcattgactg ccttttatgt catggctttc
     5161 ttattagaag cgctcgataa gtactggttc ttgacaatga tctatatagg cggtggaatt
     5221 ccaatgaata cttggctgat gaaaggatac tttgacactg taccaataga tttagatgaa
     5281 tctgctaaac ttgatggggc agggcatttc aggatttttt tccagattgt attaccgctt
     5341 gtcaaaccga tgctcgccgt tcaagcatta tgggctttta tgggaccttt tggggatttc
     5401 atgttagcta agttcctgct tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa
     5461 acatttattt ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg
     5521 attgcactac cgatttgtct attattcttc tttttacaaa agaactttgt taccggcctg
     5581 acatctggcg gaacaaaagg ataggagcag agtatcatgc aaacatttcc gttaaactat
     5641 ttttccgcat tacgtacgcc aactcaagtg tttgcgggac gcaaactatt atcatggcct
     5701 aaatttttct tgatatttgt atttttagtt tccttgatgg tcatgcctgt gactcttttt
     5761 tatgccaatc agatccaagc gattccatta gaacaatttc tatccgttca ttcgctgatc
     5821 gatgaacaag gaacccaaaa attttctgaa cttgaattat ctgaaactgg gttacaagca
     5881 tcgcaacaaa cgatagctgt gacccctgaa attttggtag gtgtgtcttt atctgagaaa
     5941 caacaatccg atcatggaac ttttattgat tttgagaagg aacaatgggt gatccaacaa
     6001 aaggataaat ctggtatacg ccgctatacg atgaattata gcccatcttt ccaaccagat
     6061 tctgtacgta cgcccgaaga tttccaacga tttttagaaa gagaatttta tgccagtaat
     6121 cgtccaacaa tcattttatc ttatagttta agtcttggtc ttgtcctatt tgtcatgacg
     6181 agtctcattt tattcggggc ttcctttttc ttgtggatga caagaaaaag ccaactttca
     6241 tccattcata cattcaaaga gagtgcaaat ttgatgctga atgttatggg aataggttcc
     6301 atggcagcag cagttgtagg gtttatccac tttgacttta tcttgatgct aagtgtccaa
     6361 acaacgataa ctgtcttgct gcttttatgg gtttttgtca aaacaagatt caaagataag
     6421 cgtgtcgaat gacaagttta agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat
     6481 aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc ttttcttttt
     6541 tagtattaac gtaatagggt agttttttta aggaggaaaa tcatgttaga gaaaacttta
     6601 tacaatcaat tattttcacg ttctttctcg ctccctgtcg aagtaaatta ttgggacggt
     6661 acaacgaaac aatacggaga tacggacact cctccccaaa tcaaaatcaa tatccatgaa
     6721 gaaatcccaa tgaaagagat tacaagtaac gcttctttgg cactaggaga agcctatatg
     6781 gatcatcgaa tcgagatcga gggaagtatc caagcattga tcaacgatgc ttatcagcaa
     6841 gcaaacagct ttttacgagg aaaagattat ctaaaatggc tgccaaagaa agaaaaacat
     6901 acgaaacaaa aaaacaaaga agatatccat gcgcattatg acttaggtaa cgacttttat
     6961 aaactttggc tagatccaac tttgacttat tcttgtgctt atttcaatac acctgaagat
     7021 acgttggaac aagcacaaat caataaagtc catcatatct tagacaaact ctttatcaaa
     7081 gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa cattgatgtt cactgctacc
     7141 aaagagtata atgtcaaggc aactgggatc acactaagtg aagaacaata cgatttgatt
     7201 accaaaagaa taaaagaaga acatttagaa gataaatgcc gtgtattatt aatggattac
     7261 cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg taggtatgtt cgaacatgta
     7321 ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag atttattgaa accaaaagga
     7381 acagctttga tccatggaat cagccgacaa caaggcggtg cgacaaatgc ttggatcaat
     7441 aaatatattt tccctggcgg ttatgtccct ggtgttactg aattagttga taatattaca
     7501 aaaaacgatt tgcaactgat tgatttggaa agcttacgtc gtgactacca attgacatta
     7561 gaacattgga caaagaattt tcatcaagtc caagatcaag tcacgaaaga aatgggtgat
     7621 tccttctatc gaatgtggga tctatattta caagcttgtg cagcttcatt tgaatcaagt
     7681 aatatcgatg tgattcaata tttgcttgtt caacctggaa acaatgacat cccaatgcat
     7741 cgtgcataat tgaggcaaaa gtactgttta aagactattt aaaaagaagc taagttttat
     7801 gatgctccag aagtgaactt ttttgtctaa cttctaggat tgtctctaaa gcttagtttc
     7861 tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca tgacagaaaa
     7921 ttttttcttc gttatacttg taatagaata ctattttata acaaagggga tcgaatcaat
     7981 gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg cttgtcaatc
     8041 aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc gctcttcaca
     8101 ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa aaagttctag
     8161 tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg aattgttcca
     8221 agataaatac ccagatactg caattactag tttggaatta gatagtgatt ggggatctta
     8281 cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa ttgatgctca
     8341 aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac aaaatggtca
     8401 aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg aagcagcaaa
     8461 aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg ataaagattt
     8521 aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat cagtcaagat
     8581 caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat ttcttcggaa
     8641 aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt gtttgaagga
     8701 gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa tcctttgatg
     8761 aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc tcattcagaa
     8821 gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca
     8881 cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa cttaggtttt
     8941 tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg
     9001 ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct tgagaaaata
     9061 gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga
     9121 aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga agtattcagc
     9181 atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc tcattttgat
     9241 cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat caacagtgtg
     9301 ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg
     9361 ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt
     9421 tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg gacaggacaa
     9481 gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac agagcttcat
     9541 aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat tatgtcagaa
     9601 aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc atagcaaaga
     9661 gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct gttcattcct
     9721 gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga ggcaaaaagg
     9781 aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa gaatttagtt
     9841 ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat gaattgaatg
     9901 cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt ccccagtcag
     9961 aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg
    10021 gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct catacatcta
    10081 attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc aatgcgccag
    10141 agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga aacccttgga
    10201 accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa
    10261 tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc gcttcgtttt
    10321 gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca tatgcttgga
    10381 gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga gacacggagg
    10441 ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta tcgccagttg
    10501 agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt gattcaccga
    10561 tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta gatttccttg
    10621 gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt aaactgatca
    10681 cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg attgaaacag
    10741 gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact ttgttccaat
    10801 atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg gatgaagcag
    10861 caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca acgactgcat
    10921 ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca cagatggaac
    10981 acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg tttgaggaga
    11041 gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca gcaatcagct
    11101 tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg gctgccagag
    11161 gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat cagttccatt
    11221 taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa aaagattgtt
    11281 tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg ccgctaatca
    11341 ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg
    11401 cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt atgatgtaga
    11461 tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt
    11521 ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga acgattttgg
    11581 tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat attcatttga
    11641 cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag atcttaaagg
    11701 gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa aacacggagc
    11761 taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg
    11821 tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat ctgctttacc
    11881 agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt
    11941 attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa
    12001 agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg tttcagtcaa
    12061 accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcccct aattgatttt
    12121 tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac attagggatt
    12181 ggcttcgtgt cgtttctttt aggaaatttg gtagggttgc ttttgacagt tttaggtctt
    12241 ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt tagaggcatt
    12301 cccgcactag tcctgctgtt tctgttatat ttcgggttac cttatcaatt aagtgcctta
    12361 acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga aatctatcga
    12421 ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc tggtttttct
    12481 ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc agtaccggca
    12541 ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg
    12601 ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta tttttctctc
    12661 tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag
    12721 tatttagaaa aacgttctat ccatcagtca atctgaaaaa tagaaatggt gctagcgtgg
    12781 ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta aaaccatttc
    12841 atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc ccgctaacca
    12901 tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg gttgtagaag
    12961 aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg acaaaatttg
    13021 atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat tttcgctcta
    13081 cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt aaactatatg
    13141 aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg
    13201 ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca gcaaagaaag
    13261 agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta ttcagggaag
    13321 taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaaaagat caattgtaca
    13381 aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga tattatgaac
    13441 ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc
    13501 ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg cctacagagc
    13561 cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg acctattagc
    13621 attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag tgatcgaacc
    13681 agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcc
    13741 aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc agcatgtgca
    13801 cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa cgccagaaaa
    13861 tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac agcactaccc
    13921 aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt tgataccaga
    13981 tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg actcttttca
    14041 aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta gtgatgacta
    14101 tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg aaggtagccg
    14161 ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt atattgatgc
    14221 ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg ctaaagacca
    14281 agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc gtcaccatca
    14341 tcaactcttg attacttcag gaatgattga attattggct ctttaccagc aacaaaaatt
    14401 ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac ttattagtca
    14461 cctgctagat catccaaaag attggcttaa atattattca aaagttcagg aactatatga
    14521 agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga aactggatgt
    14581 atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt aatgccgcag
    14641 cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag
    14701 aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg gaagatcaaa
    14761 ttgctggaac agctgcatta gtctcaggcg tggatcccgt ttacacaaaa atagatggta
    14821 gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta gcagaaagta
    14881 ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt gcagccagca
    14941 aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg gaaaagacca
    15001 tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa cacggcgaaa
    15061 gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa gataaagaca
    15121 ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag aagtggccag
    15181 cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg aattttagcg
    15241 tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg acatagcttt
    15301 tgtcacgatc tctcggctta tcctacgagt attattcgat acatttatat caataatcct
    15361 ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac taaaagtgag
    15421 gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc cagtctggct
    15481 gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt ttctttagag
    15541 atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg ccaaaaacca
    15601 tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat ttctgcttta
    15661 ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt tcccttgatc
    15721 atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt ttccccaggt
    15781 cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat agcttctgcg
    15841 agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt cattgtcatt
    15901 tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa
    15961 gtaactttgc ttttttgtat tttataccag caagccagtc acaatagcac ttaaaaagct
    16021 gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat ttcctttttc
    16081 ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt tggcaaatga
    16141 aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat taccatttgc
    16201 taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga tactaccggc
    16261 atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa agatgtaacc
    16321 taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga tcatggcaat
    16381 caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga aaccatcaag
    16441 aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc
    16501 atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat aaccgccaaa
    16561 taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca tataagatcc
    16621 gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca aacgtttctc
    16681 agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc ctaaaatagc
    16741 agaagctact ccattataag attccaattt gcccatcccg ttgattttac ttaatcctag
    16801 accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac cgataatagc
    16861 agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc
    16921 tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat gatcaaacat
    16981 ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata cgaaaccaag
    17041 aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt ttttgttact
    17101 gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta ataaataact
    17161 caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa ataaaatact
    17221 aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt tccattagaa
    17281 aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa
    17341 aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta aactttttct
    17401 attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg attcctagtt
    17461 ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct tgtgctccct
    17521 caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact gcactgattg
    17581 ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga agtgtctttg
    17641 atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg tcatctgcga
    17701 tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc atcaatgaac
    17761 ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca gcagaaggat
    17821 cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg actaccacgt
    17881 ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga ttaactgctt
    17941 cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga cggatgattt
    18001 cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct acacgattca
    18061 cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt tcaataatac
    18121 gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact ttattgtagg
    18181 catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg gctgcttcat
    18241 aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt tcgaacttgg
    18301 agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag caagtagcga
    18361 gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt gtttcaatat
    18421 actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact ggatagtctt
    18481 cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg ataaagactt
    18541 tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat aagccataac
    18601 cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca taatcaccac
    18661 ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca gcttgatctt
    18721 tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt acacgtttcg
    18781 catgcttgtc gtcaactaac ttcccaccaa attctaaata taatttgtca tagtggttca
    18841 ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca aaaccaattt
    18901 ttttcactag gttccctcct taagtactaa agcatttttt catttttttg aggattcgtc
    18961 aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag
    19021 atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa aaaaagacag
    19081 aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta tgaaagaatg
    19141 cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga aacgcagcta
    19201 actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag taaataaaaa
    19261 ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact agttgatcga
    19321 aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc cgctcttttt
    19381 tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa aaacagatga
    19441 gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg agccagacga
    19501 actagaggag acgaacgcgg atcaggatac aggacaagaa tcgtcttctt tttacgatat
    19561 caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg cttttgaaaa
    19621 agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc gattgaatga
    19681 agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga tgcgtaagat
    19741 ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca tctcgccaac
    19801 gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg gtattgatgc
    19861 aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga aatattccca
    19921 accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg tgatcactgc
    19981 tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac aaatgactga
    20041 tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt
    20101 agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt atattgagac
    20161 gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag cagaccagct
    20221 tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga
    20281 gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg agaatcctaa
    20341 taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca gaatggacaa
    20401 aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa gaacaaacaa
    20461 gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa atacatgaaa
    20521 aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct tacgaaataa
    20581 ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc gtcagtttga
    20641 ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg atcgaatcag
    20701 attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa ccaatctcca
    20761 cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa ccgttgaatg
    20821 atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga catttttcga
    20881 tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta ccgaccatcg
    20941 atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag acaatggaaa
    21001 tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg gatcgagtat
    21061 tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta gcccatgcaa
    21121 gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca cttcgtttga
    21181 atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt atcgtggcaa
    21241 tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa gtacgattag
    21301 ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct
    21361 catctggaga ctacccacga tctgtcattg atttctttat caattacgaa ttcttggtcc
    21421 cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca ttcagataat
    21481 tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct tttctgtatg
    21541 tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga atgtccacaa
    21601 ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc
    21661 aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta ctttacttaa
    21721 gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt ttttattttg
    21781 gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg agtttgtttt
    21841 caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga gtctgttttt
    21901 ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg ttatatcgtt
    21961 tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg cacagtatag
    22021 gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac aatcattcag
    22081 gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag tatactcatt
    22141 ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga ggaagtaaaa
    22201 acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa
    22261 gtggtcttca aaaaaggcga tgatttactg agtattttga aacgaaattt tgaagtcaaa
    22321 gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa aaatcaactc
    22381 tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga gatcaaattg
    22441 aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata aaaaactgtt
    22501 aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga aaataacatg
    22561 gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc taatattcta
    22621 gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat gctaaacgaa
    22681 agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg tgataaacaa
    22741 gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa atactttttc
    22801 agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt gatggaagct
    22861 tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt tatagcaata
    22921 ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa
    22981 gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa
    23041 atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt ggctttagca
    23101 ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag cggattgctg
    23161 atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta tagtctgatc
    23221 atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt tgaacgtcct
    23281 gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga gattttagtt
    23341 gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt catcaatgat
    23401 attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata cattgaaaca
    23461 gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa
    23521 atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga atttatgtta
    23581 caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa
    23641 agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag ctatatacca
    23701 gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct cttatcagga
    23761 ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc tatgcttacg
    23821 acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt acgctataca
    23881 ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt tttggctgtc
    23941 ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt ttcaactgac
    24001 ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac tgcatgggcc
    24061 ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc tgaccttgtc
    24121 cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa aagcgcacta
    24181 ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt attattccaa
    24241 aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt tatgctggag
    24301 aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag
    24361 ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat atttgctact
    24421 tttagttgct tgtctgttca tcgtggcata tatcccgcgc ttgttcccga tgctttattt
    24481 tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac cggtggcatt
    24541 atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg atattgcttg
    24601 gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa cacgttcgat
    24661 ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt
    24721 tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct atccgaactg
    24781 catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt
    24841 ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt ccttctatga
    24901 ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg gaatttaaat
    24961 gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc tatgtaaagt
    25021 tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc aagatggtat
    25081 gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc tgatgcttgg
    25141 tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa aagaaggaga
    25201 taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg tcatcggtat
    25261 cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca gtccgattgc
    25321 taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga ttgctatccc
    25381 aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc cttatggaac
    25441 ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta cttattactt
    25501 gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag tcgcttcttt
    25561 cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa aagacttctt
    25621 cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc gatctgcctc
    25681 tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca ttactatcgt
    25741 tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc cagagatcgc
    25801 agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata aattgacatt
    25861 gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat taagcacgat
    25921 gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt cgattgcgtt
    25981 attgattctt cttccttcat tgactggaat gtctttatta gctggaccac tatacacatt
    26041 gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc tagcttcttt
    26101 gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc accatcgttt
    26161 tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag taatcggatt
    26221 aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg gtattgtctt
    26281 tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag caaagatcag
    26341 caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat gttttgccgt
    26401 tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa tgatttattg
    26461 tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg gaaaaaatgg
    26521 gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg ttaactaatc
    26581 gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg tttttctatt
    26641 ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt ctggaaaaat
    26701 tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata gttcggcata
    26761 tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc ctttgattat
    26821 ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt atggactttt
    26881 aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag ttttgggttt
    26941 atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc tttcttcagg
    27001 gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg gagtaacggt
    27061 cattccttat gactctaaga tcactagtat gccgatggga cacatggtag aagctgcctt
    27121 agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg atcgaatccg
    27181 agataatacc tatgcttatc tgattgtaga tgatttgaac aatttagtac gaggaggacg
    27241 tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga tcctgacatt
    27301 ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag catttgctcg
    27361 agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca aactttacgt
    27421 gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc agaaaaaata
    27481 tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac atttaggtga
    27541 aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa aacataccaa
    27601 aaata
//
LOCUS       DAYYBG010000025        27455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000025 DAYYBG010000000
VERSION     DAYYBG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.025"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACHAUN_001119"
     CDS             275..1753
                     /locus_tag="ACHAUN_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8459836.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2030..4090
                     /locus_tag="ACHAUN_001120"
     CDS             2030..4090
                     /locus_tag="ACHAUN_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8459837.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4173..4742
                     /locus_tag="ACHAUN_001121"
     CDS             4173..4742
                     /locus_tag="ACHAUN_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8459838.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4917..5723)
                     /locus_tag="ACHAUN_001122"
     CDS             complement(4917..5723)
                     /locus_tag="ACHAUN_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459839.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5861..7375
                     /locus_tag="ACHAUN_001123"
     CDS             5861..7375
                     /locus_tag="ACHAUN_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8459840.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7778..8761)
                     /locus_tag="ACHAUN_001124"
     CDS             complement(7778..8761)
                     /locus_tag="ACHAUN_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8459841.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8766..9413)
                     /locus_tag="ACHAUN_001125"
     CDS             complement(8766..9413)
                     /locus_tag="ACHAUN_001125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8459842.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9498..10130)
                     /locus_tag="ACHAUN_001126"
     CDS             complement(9498..10130)
                     /locus_tag="ACHAUN_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8459843.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="ACHAUN_001127"
     CDS             complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="ACHAUN_001127"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8459844.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10694..11431)
                     /locus_tag="ACHAUN_001128"
     CDS             complement(10694..11431)
                     /locus_tag="ACHAUN_001128"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8459845.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11428..12417)
                     /locus_tag="ACHAUN_001129"
     CDS             complement(11428..12417)
                     /locus_tag="ACHAUN_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8459846.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12675..13217
                     /locus_tag="ACHAUN_001130"
     CDS             12675..13217
                     /locus_tag="ACHAUN_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8459847.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13234..14319
                     /locus_tag="ACHAUN_001131"
     CDS             13234..14319
                     /locus_tag="ACHAUN_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459848.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14331..15137
                     /locus_tag="ACHAUN_001132"
     CDS             14331..15137
                     /locus_tag="ACHAUN_001132"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459849.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15138..15965
                     /locus_tag="ACHAUN_001133"
     CDS             15138..15965
                     /locus_tag="ACHAUN_001133"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459850.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15962..17035
                     /locus_tag="ACHAUN_001134"
     CDS             15962..17035
                     /locus_tag="ACHAUN_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8459851.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17463..20159
                     /locus_tag="ACHAUN_001135"
     CDS             17463..20159
                     /locus_tag="ACHAUN_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8459852.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            20224..20724
                     /locus_tag="ACHAUN_001136"
     CDS             20224..20724
                     /locus_tag="ACHAUN_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8459853.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            20736..21230
                     /locus_tag="ACHAUN_001137"
     CDS             20736..21230
                     /locus_tag="ACHAUN_001137"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8459854.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21251..22060
                     /locus_tag="ACHAUN_001138"
     CDS             21251..22060
                     /locus_tag="ACHAUN_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8459855.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            22057..22878
                     /locus_tag="ACHAUN_001139"
     CDS             22057..22878
                     /locus_tag="ACHAUN_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8459856.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22929..25028
                     /locus_tag="ACHAUN_001140"
     CDS             22929..25028
                     /locus_tag="ACHAUN_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8459857.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            25025..26143
                     /locus_tag="ACHAUN_001141"
     CDS             25025..26143
                     /locus_tag="ACHAUN_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8459858.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26182..27060
                     /locus_tag="ACHAUN_001142"
     CDS             26182..27060
                     /locus_tag="ACHAUN_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8459859.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27206..27403
                     /locus_tag="ACHAUN_001143"
     CDS             27206..27403
                     /locus_tag="ACHAUN_001143"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8459860.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8770 a         4711 c         5744 g         8230 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aactagatat aactagatat attacttaac
     1861 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
     1921 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
     1981 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
     2041 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
     2101 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
     2161 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
     2221 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
     2281 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
     2341 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
     2401 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
     2461 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
     2521 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
     2581 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
     2641 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
     2701 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
     2761 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
     2821 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     2881 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     2941 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     3001 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     3061 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     3121 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     3181 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     3241 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     3301 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     3361 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     3421 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     3481 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     3541 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     3601 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     3661 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     3721 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     3781 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     3841 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     3901 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     3961 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     4021 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     4081 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     4141 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     4201 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     4261 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     4321 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     4381 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     4441 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     4501 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     4561 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     4621 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     4681 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     4741 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     4801 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     4861 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     4921 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     4981 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     5041 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     5101 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     5161 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     5221 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     5281 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     5341 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     5401 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     5461 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     5521 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     5581 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     5641 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     5701 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     5761 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     5821 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     5881 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     5941 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     6001 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     6061 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     6121 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     6181 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     6241 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     6301 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     6361 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     6421 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     6481 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     6541 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     6601 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     6661 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     6721 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     6781 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     6841 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     6901 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     6961 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     7021 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     7081 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     7141 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     7201 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     7261 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     7321 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     7381 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     7441 ttgaacactg tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     7501 gccaagtaaa aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa
     7561 gcttttgaac accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt
     7621 ctcacgctct aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg
     7681 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     7741 cgcttctgtc ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga
     7801 tttttccgat gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt
     7861 ggaatgtacg gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt
     7921 gcataaaagt ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac
     7981 caagcagttg gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc
     8041 cacctagcat accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt
     8101 cccagtaaga agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt
     8161 ttcgtacctc ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt
     8221 ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa
     8281 gctgtctagc taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga
     8341 cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa
     8401 cttctgagat tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat
     8461 cggcaggcat cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg
     8521 tcccaggagt aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga
     8581 ctcccaaaat agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc
     8641 gattgacagc tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag
     8701 gcacttcaat ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca
     8761 tggatctatt cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca
     8821 gaaagtacta gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca
     8881 gcaactgtag agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg
     8941 gaagtaactt cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta
     9001 gttcgtttca gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca
     9061 ggccattttg ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc
     9121 gcattttcag ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc
     9181 tttccttgag agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt
     9241 ttttccacgg accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat
     9301 gtttgggaag caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct
     9361 tttatcaggt gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc
     9421 atcctttcga cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag
     9481 atcggctacg atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca
     9541 ttgcatactg ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa
     9601 ataaccgcta gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa
     9661 tcttgctgga ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg
     9721 gaaagtccag ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga
     9781 cctttataaa cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc
     9841 aaggctttcc agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa
     9901 gacaaaggtg tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca
     9961 agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg
    10021 gttgttgcat tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc
    10081 aaaatagcta aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct
    10141 tttttctaag catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata
    10201 tcagctaagg caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt
    10261 tcctgatagc cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac
    10321 atattcgtgt tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg
    10381 actgttgaaa gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta
    10441 gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc
    10501 ggatttgatt cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt
    10561 aatggcgtga taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat
    10621 ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct
    10681 agtaagatct ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa
    10741 tctcagattt actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt
    10801 gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta
    10861 tcagactatt ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga
    10921 aaccaatgat ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt
    10981 tcttcaaaac gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg
    11041 tctgttcatt ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt
    11101 tttttgccag ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac
    11161 tgtggataat cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt
    11221 ccagtgcaga ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt
    11281 gtttcgtagt cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg
    11341 cttgccctaa tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag
    11401 tcgttcctcc agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct
    11461 tccttggtgt gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat
    11521 ctcttggacg accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag
    11581 acgttgatct attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat
    11641 aaaaggcaag acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc
    11701 accgttataa gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat
    11761 gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg
    11821 tcgtacgact tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga
    11881 aaaggtaatg attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac
    11941 accgccagaa gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa
    12001 agcgttcaca cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag
    12061 cgtcatattt tttggtagag gaactaacca gtctcctggt actttttgat attggctata
    12121 accgcctgga tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga
    12181 aaaacgtgga ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg
    12241 atatgttcgt atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc
    12301 agaataggca atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact
    12361 gattgctgta tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg
    12421 tgagacactc cttttaaaat tttcttttat cataccataa agcctactct tttattcttg
    12481 ttcttccgta aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt
    12541 attccaaaag ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa
    12601 taaatttcgt atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg
    12661 attcggagga aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt
    12721 tgacacaaga agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg
    12781 aacgagattt aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg
    12841 tgactccaga agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag
    12901 aagatcatac ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg
    12961 attcgaatga aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata
    13021 agacgtttga accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt
    13081 taagcttagg agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga
    13141 caaaacaaca tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa
    13201 cgcaatcata tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat
    13261 caagtagtga aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa
    13321 caaggaaaat tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt
    13381 attatagctg gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg
    13441 aatgatttgc cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt
    13501 cctcatatga atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa
    13561 tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa
    13621 aaacgggaga ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca
    13681 attgtaaatg aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa
    13741 ctacgtacag atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt
    13801 atttttgtta ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg
    13861 aataaaggga aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat
    13921 catttcgttg ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat
    13981 aatctagttg agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat
    14041 gaaccagttg aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga
    14101 aaactagtcg taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt
    14161 tatgatgaac agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc
    14221 aaagtaggat tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa
    14281 gaagaattcg atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa
    14341 tgcgcaggat ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg
    14401 tattaatgat catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt
    14461 atctatctta ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg
    14521 catttttgat cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa
    14581 aactgaagca caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc
    14641 tgcttaaagc ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct
    14701 ttgattttat cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg
    14761 tggcaacgta tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga
    14821 tgaatccttc tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc
    14881 gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta
    14941 ttccttctct ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt
    15001 taggaacggc tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa
    15061 tcggtgtgat tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa
    15121 aaggaggtcg ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct
    15181 ttttattctt atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa
    15241 cgatgaacag ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac
    15301 gtttgatcat tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca
    15361 cgatcatcgg gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga
    15421 cagcgttgtt gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta
    15481 gtttcttgat tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt
    15541 cccatattgc tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa
    15601 tgaacgattc aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca
    15661 aaaatatcat tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta
    15721 cctattctct tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc
    15781 tctcagtaga gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa
    15841 gtgcgttagt tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag
    15901 aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc
    15961 gatgaaaaaa ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct
    16021 tggtgttcgt caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta
    16081 taactgggga gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata
    16141 caaagtgaat tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg
    16201 tgggacaaac tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga
    16261 aaaactagta cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg
    16321 atttttagac ttggattttg atccagataa tcagtattcg attccatatt tttggggaac
    16381 tttagggatc gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga
    16441 tttgtggaag ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat
    16501 ggggctttct ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagctgac
    16561 agaagcagca aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga
    16621 gatcaaaatg tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc
    16681 tgccgatatg atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa
    16741 tctttggttt gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga
    16801 tttcatcaac tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta
    16861 ttctacacca aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca
    16921 attctatcca tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa
    16981 atacctaggc atctataatg acctattctt agaattcaag atgtatagga gataatggca
    17041 aaactcagta agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct
    17101 atctgagttt tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt
    17161 tattttttct accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg
    17221 cccgagacgg gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa
    17281 actatgcaaa tatacttaac ccttctgtaa tctattaata aaactaatat atgaatccag
    17341 ttcaatattt gtagctatga aaaaacataa aaatcatatt agataaaaac taaattggta
    17401 gataaattat tttgtcagat atctgaggca aatattcgac tatttgtgtt tcaaagtaaa
    17461 aattggaata caaatataga atagacatat tgcagccgat agtttattat gcttttagac
    17521 acattaaact ggaggaaaaa atgaaaaaga gcgctaaaga tcgagttttt gatgagctga
    17581 tacgactaac agggaatatg tcttctgtca ctgccgagaa aatttcgaag aatctaggta
    17641 tctctcgtca gaatgccagc cattatttga cgagattagt ggaagataaa aaggtcgaga
    17701 aattaccggg aaagcctgtt ttgtggaagc ctttagatga atatgcggtt gtagataata
    17761 cggagcaaat aaacgaagcc tttcattctg tagttggtca tgacggaagt ctgagagagg
    17821 tcattcaaaa atgtatagcc gctgtcaaat atccgcctaa cggactcagt gtattaataa
    17881 atggagccac gggtgttgga aaaagttttt tagccacaaa aatttttgaa tacgctatcc
    17941 atgagcaaat tattgaaaaa gatgcaccat ttgctatctt aaactgtgcc gattatgcgg
    18001 acaatcccga actgctttcg gcaacactgt ttggatacaa gaagggggcc tttacggggg
    18061 ctgaaaagga taccgatggt ttattggcaa cggccaacaa cggctatctt tttctcgatg
    18121 aggtccatcg cctatcgaaa gaaaatcaag aaaagctctt tctatttatt gatactggaa
    18181 actatcgtcc agtcggtgaa aatgtaaatt ggcattctgc taaggttcgt tttattttcg
    18241 ccacgacgga aaaaggtgaa aattatttat tagacacctt tgatcggcgt attcaaatta
    18301 gtgttatgtt gccaactttt gaagatcgcc ccattcgaga acgattagag ctgattcaat
    18361 tattttttca aaatgaagcg gatgttttac aaaaagatat tattgtttca agagaagcct
    18421 tgtctttaat gttatctcat ccctttagtg ggaatattgg taagctgaag aatatcatta
    18481 aaatcagttg tgcggatgtg tatagtcgaa cacaagaaga accattaacg attgggaaaa
    18541 atgagatctt gattcagtta aatatgctgg aatccgaagt agaaagcttg ccgattgcta
    18601 gtttattgat aaagaaaaat caaaagataa aaacggatcg aatcaaaccg tttgattatg
    18661 attcgatttt ggaaaaaatt actcagctga taaatgaagg taatttcaat gaaaattttt
    18721 cttcgataaa gaatgaagta cagcatttgg tgcatcacat tgataaggat tcttttttat
    18781 caatgagtag aaaattgatg attcagatgt ttagaaaggt ctgggtatcc gttttaggaa
    18841 aaacgtacgg attgacgacg agtggacctg ttgcagaaat tattagcaga tcctatgtca
    18901 tttccccaag ctataagaca gacttaaccg ggataaatcg tacattagct gcaaatctac
    18961 ctcggacgac ttacttatgt aatcagttct tggagcattt accaccgctc tcagaaaagg
    19021 acgagaactt tctaagactg cttattgtcg tgactttatc tgattatgta gatgaatcca
    19081 tcgagctgaa gggcctgttg cttgctcacg gagatagtac ggctagcagc attcaagcag
    19141 tagtgaatca attatgcggc aattatgttt ttgaagcgtt ggatatgcca gtagagagta
    19201 atgtgtcaga gattgtcgaa tcgacaagga atttcgtgaa acggcaagtg aatacagata
    19261 gtctagtgct gatcgtagat atgggctctt taaatcaaat ttacacacag atcaagaacg
    19321 atttaaaagg ggagttactt cttctgaata atttgacgac ttccattgca cttgatattg
    19381 gcttgaagat gacgatgaat gcgccattta aacagattgc ggaggatgca gcagacaagt
    19441 attcaatcaa tgttcaatac tttgaaggct tttctcaaag tagcaacatt atcatttcct
    19501 gtatgtcggg tttggggatt tcggacaaat tgaaagatgt ttttcgtcat catttatcgg
    19561 atgaatccat cgaagttttc acgaaggact acagagaact acgtgaatta atcgaccaaa
    19621 atgatgaaac ttattttgac aagacaaaat tggtcatcac gacttcggat cttccacgtt
    19681 ccttttcaat tcctaatatt aatgtttatg acatcttaga cggtgatgga gcagccaatt
    19741 tggagcatat tttgagtcct gaaatgaagc cggcagaatt taaccgcttg attcaggagc
    19801 tagtaaaatt cttttcgttg gaaggaattg cggaccgttt gaactttctg aacccaacga
    19861 tcgtgatgag tgaagtggaa acagtcattt ctaaatatga aaattactat catttcactt
    19921 taaatggccg tgtaaaacta aatctctata tgcatacggc attgatgatg gaacgtttat
    19981 tcttggctcg gacagaaaaa tctgaagaac cgaattatga tttgaaacca gatgaagatg
    20041 aattttatta tgtttctaga agtatttttc aatcaatgga gatgaaatat aattttaaag
    20101 tcaatgctta tgagctttcg ctgctctatg aattattagg accgtatatt caaaaataaa
    20161 gtgtctaaaa aagtgtctaa tctgtttttt ttaggttaga cacttttttt aatgtgaatt
    20221 aacgtgatta aaccaatgtt tttaaaagtt ggcacaggaa ttgcttatat aaaagtgaac
    20281 agaaagaaag gaattaacca tatgaagaaa attctaatag cgactcacgg tcacttagct
    20341 tcaggctttc ttagcagtat tcaattactg acggggaagg ttcaagagat tacggtgatt
    20401 aatgcttatg tggatgagtg tgattttgaa caggagttga atgtatttgt aaaccaggtg
    20461 aacgagcagg atcagatttt tgtttttacc gatttatttg gcggaagtgt caatcagaag
    20521 attaccaaaa cctttttgga gaaagagatt gctgtaacta ttttgtctgg ctttaatctg
    20581 ccaattctgc tcgaaatagt attggcaacc aaggaattaa caaaggagga tattcaggaa
    20641 ctggtaaata aatgccaaca ggaattaaag atgaccattc ttgatgaggt acatgtagta
    20701 aatgaagagg aagatttttt ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg
    20761 aacgattaat tcatggacaa gtagcgatga cttggacaaa gagtctaaat ttaaccgggt
    20821 tagtagtagc aagcgatgag gcggctacca acgatattca aaagatgaca ttgaagatgg
    20881 ctgcaccatc gaatgtcaaa gtcattatta aaacagttga tgacgcgatt catttgttaa
    20941 aagatccgcg ggcgaaggac atgcgcttga tggttttagt ccctaatgtc aaagatgcgg
    21001 taaaagttgc gaaagctttc ccaacggaaa tcgaattaat gaacgtgggg aatgctggga
    21061 aaatgacacc gccatcggaa gataagaaaa ccttatcaaa ggaagtgatg ttaacgacgt
    21121 cagaaattca agacttaaaa gaattagttg aactttatcc agataccgtt tttcagccta
    21181 caccggcgat ggaaaaacgt tcagctatct ctattttgag tgatttttag aaaaaggaag
    21241 gagaaaaatt atgtatgatg catttgtagt cgctttggcc gtttttattg gagttgctgg
    21301 acatgaaatg ttcgggatgt ccatgctgag tcgtccgatt gttgttgctc cgttagcagg
    21361 agcgttgatg ggggatttgc agacggggtt gattgtcggg gcgtcactag aagccatctt
    21421 tatgggtgtc gtcaatatcg gaatctctag tacggcggag ccttctttgg ctgctgggct
    21481 ggctacagcg ttcgcaatca aaatgggagg aaacgtggat gcgattattc ccattgtctt
    21541 tccactagca gtattaggat tacagttaat gaatatgatt ttttcttttg tatgtggacc
    21601 gatggctgct aaatttttca aatttgcaaa atctggtgat caaaaaggaa tcatcaatct
    21661 gcacttcttt atgtggtttg tccactatgc gctttatgct cttattccat tctttgccgt
    21721 tttatttgga gcagatgttg tcaaaagcgt cttggataat attccagagg tcatcatgaa
    21781 cggattaaca gtcgctggaa atctgctgcc ggcagttggg atggcgatgc tgttacggat
    21841 gctttggagt aaaaatattg ctgtgtggtt tttctttggg gccgttgtta tggcgtatct
    21901 cgagttacca ttgattgcaa ttgccgcagt tggaacgatt attgcaattg ttatagccca
    21961 aagagatcta caactaaaaa atatatctgt tcatgcagtt caaagcgatg ctgctgtaaa
    22021 tgcaacgctt gaccaagaag aggaggattt ctttcaatga aattagctga tggaatttct
    22081 aaagatgaga aaaaattggt tcggaagatg ttttggcgct cgatgactat gtatgcttcg
    22141 gtgaatccgg tgacgatggg cggcggtggt ttcgcctatt ctatgatgcc cttcattaat
    22201 catttctaca aaagtgaaga agatcgagct gcggcgctgg aacgtcatac acagtatttt
    22261 tcgacgacca ttccttgcgc gtcctttgtc atggggattg cggcatcgat ggagaaggaa
    22321 aattctgaaa aagctggttt tgacgtggaa agtatcaacg cgatcaaaac gagtttgatg
    22381 ggaccattgg ctggaattgg cgattcattg ttctggggag tttggcgtgt cgttaccgcg
    22441 ggactagcga tcaacttagc caatgccgga aatattttgg caccaattat ctttttagtt
    22501 atgttcaata tacctaatta cttgatgcga tactatggcg gcgttttagg ctattcatta
    22561 ggctctaaat atattgaaaa gctgtatgcg aacgggttaa ttgaaattct gacaaaagct
    22621 gctagtatct tgggtctgat catggtaggt gcaatgacga tcagtatggt caaattcaat
    22681 accgttttga gcttttcaat gaagggcgaa gaactaatga atgtccaaga aatcctcgat
    22741 agtattttta aaggaattgt accgttattg gtaacattga gctgtttcaa attactagat
    22801 aaaaaagtcg gcgtcatcaa aattattttt ggaatggtcg tcttgggaat tttattgtca
    22861 ttacttggaa tcgcatagta ggaagctgca gctactaggc tgcagttttt ttagcaagga
    22921 gggaaaagat gactattaat gaagtaattt attcacccaa acacccattt tttaatgctg
    22981 agttaaacga cggaaatgtg actacttcaa acgaatacag ctactatcgg aatattcctg
    23041 atgtaaaggg attgaccttc gatgtaagta atcaaaagac attagcaaac cttgatgttt
    23101 gcgggacgat tcaaacgttg accttttatc gagaaaattt actaactgag gaaaaaccag
    23161 gtgtttgggt caataaacag ctctctcaaa ccaattcgtt atccttaaag gttgaagtaa
    23221 acgggattgt ctaccatttg tcagaatcgg atcatcgtat tgaagtcgat gtgattcaag
    23281 actgtttgcc tagatatatt catcattatt cgacgtttaa ggtcattgta gtatcctttg
    23341 ctcccatact ggctaataag cgtttaagta tgcttgttca acaggtttac attgtgaatg
    23401 aaacacacga aagccttcaa gtttcagtga aagatgtgcc ttcgtatcaa gagaagtatt
    23461 cggatcagca aaatgtgttg atttctcaaa aaggaaataa ggacacgatc gcacaacagg
    23521 aagtggcaag cttcgccatt gcttttgtgg acccaaatgc ttttgaagaa gtaatgactt
    23581 ttcaggaatc tgatatcgaa aaatggctga aggaaacatt gaactatttt gctcagctat
    23641 ttgggcagct gtcaatgcca aacgatagag ttgtccatct ctatcgtcgt gcactttatc
    23701 aaagcttttc tagtttcggt atgaatcgac agggacaggt cgtcggcagc aattggggca
    23761 gctatcctgc tactaatcga atttggaata aggatatgta ttacgcatca ttgccattca
    23821 ccatgtttga accggagtta tgtcaaaaaa cggttctatg gtttgatcaa tatggcgtta
    23881 agtttcctgg ttcaaaattt acaggaggta tcaatcattc tctgagtaat tcgttatcca
    23941 gtgcgttact ggctagtctt tattttgaac atactgggga tacaaaattc tttgagcggt
    24001 gtccgcaggt tctactgaat gcttcaagaa ttattgagga catattagct caacgtcggt
    24061 caggggaacc aatgcttttt ccatctgttt ggttatcaga cgcctttgca cttggaaagt
    24121 atcataccgg cagcaatctt tgtctttgga aggcctgtat tggacttgca gaaatttttg
    24181 aagccctaga acaactgagt cttagcaagc gatacaaaaa cattgcctgt aaactaaagg
    24241 aatcaatttg taaacaaatg actatcgatg gtccttttgg tgagcaattt ttggagggaa
    24301 tcggtgatga ggagaaaaca acctattcag tgaagcacta tcaaaaaccg attcttgagc
    24361 aagggttaat ctttttaagt gatgtgattg tggatggaaa aattaattta ttgatgcatg
    24421 atggagaaga atcagacact acgttgatgc ctttttatca atttttagat aataaagatc
    24481 atttatatca gaacaccatg gccttttctg ctagttcttt caatccaacc tatagcgagg
    24541 aaatcaaagg catcacttgg ggattagaat ccggcgccac ttttcctggc tttatcactg
    24601 ttttaatgag cgagctgcac aaccctcaag ctttcgtaac ccgattagaa gaactaattt
    24661 gtttggcaga tttagacggt tcgtggtggt ggtggccgta tcgcttagag gctcaacaag
    24721 gagaagtcgt gcgagacttt ggctgcggca aatgcggctg ggcatcgggt ctttttgtca
    24781 gcctaatgat cagtcaatat ttcggattga aattcaaaaa aggagtattg caaattgatc
    24841 ctgttgataa tttaacgttt tcttggaaga atttgaagtt cggtcaagcc ttcatttcga
    24901 ttgaatgtac gcaatccgag ttgagtattt caaatttgtc agaaaagcca ttgaaaataa
    24961 gtgatacgaa aaaaattctt cacgtagaaa agggaaaaac gattcacatt caaaggagaa
    25021 aaagatgagt atactaaaaa atattaccca atttaaaaaa atccagagtc gagcaatcac
    25081 tcccgaaaat ccaacaggcg agaaaggtaa agctgcgatg ttgccaagcg agctaggacc
    25141 aagccgcaaa ggtcgtggcg caattccgct gacttctggt gaagaaactg tgattgcgga
    25201 tattactgga acgggaataa ttcgtcatat gtggatgacg attcgtgatc gaacagaatt
    25261 tggcagtttt gttttacgtg atttaatctt aagaatttat tgggatgggt cggatagccc
    25321 tgctgttgaa agtcctttag gggatttctt ttgtaatggg tttggagagc gttgcgaggt
    25381 gaactcgttg ccgatagtgg ttaatcctac agggggcttt aattcctatt tcgaaatgcc
    25441 tttccaaaca tccgctaaaa ttacgatcga gaatttacat ccgaaggata tgtcctcatt
    25501 tttttatacg ataaattacg ctttggtgga cgaattggaa gaaaatagcc tatattttca
    25561 cgcaaattgg catcgggaac gcattaccaa attacaggag gattataagt taattgatcg
    25621 attaaagggt caaggctatt acgtgggtac ttaccttgcg cttacctgcc tagaacgtta
    25681 ttggtggggc gaaggcgagt ttaaattcta cattgatggg gatagtgagt atccgaccgt
    25741 aacctcaaca ggttcagaag attattttgg cggggcgtgg gcctttcatg agtacgatga
    25801 gttcggacgt ccacatgcga aaaactattc aacagccttt ttagggtatc cttaccaatc
    25861 aaatagagat gccacaagag atcgttttga aacgggaaaa ttgaatgccg ttcatgcctt
    25921 tggtgacgac gcaatgccac gacatggtct ttatcgttgg catcttcaag accccatctg
    25981 ttttaccgaa gatttatcgg tcactttaca acaaatcggc aacgatgatg ttcgcttatt
    26041 tgagcggcaa gacgatgttg ctagtgtcgc ttactggtat tcaacagaag cttcaggaaa
    26101 tactaaacgt catttcactg atcgagaacg cttgccgcgt taatgaagtg tttttcacat
    26161 attcgaaggg aggaacctat gatgatatat ggggcaattg aagctggagg gactaaattt
    26221 gtctgtgcag tcggtactga ggaattaacg attttggaaa gagtcagttt tcccacaaca
    26281 actccagttg aaacaatgaa gcaggtcatt gattttttta aaccttttaa ggaacaattg
    26341 gcaggcatcg gggtcggctg ctttggtcca attgatattc aatgcaaatc aacgagtttc
    26401 ggtcatatca catcaacacc aaaacttgct tggcaaaatt tcaattttgt aggaacgcta
    26461 aagcaatatt tcaatattcc gattgaatgg acaacagatg tcaatgcggc ttgctatggt
    26521 gaatatgttg ctggttctgg caaagggtta gcaggtgttg catacttcac tgtgggaaca
    26581 ggtatcggag gtggtgcctt gttcaatggg actttcgtgg aagggttcag tcatccggaa
    26641 atggggcata tgttgatcaa gcgacatcca aaggacaatt tttcaggaaa ctgtcccttc
    26701 caccaagatt gtttagaggg gatggcagca ggaccagcga ttgaaaaacg gttgggtgta
    26761 aaaggccaga atttattggc tgacgattcg ttttggcaga ttgaagcctt ttaccttgcc
    26821 caatgtgcat acaatacaac actgatgttt tcgccagata gaattatttt tggcggtggt
    26881 gtaatgaaac aagaacacat gaagaaaaaa gttcaggata aatttgttga gcttataaat
    26941 ggatatgttg aaataccacc aattgatagc tacattatta cgcccgaatt aggtgataat
    27001 gccggaataa tcggcggatt agcattggcc agaaaagcgg taagaaataa gcaaccataa
    27061 ttaatgaatg tttcattgat actcatgtca aaagtattta accgagtaat caaatgatca
    27121 aatgaagttg gataaagcga aaatgaattc aaataaaaag gctctaagtc aaatcgtgtt
    27181 gttttcatta aatcactgaa aggaaatgct tctgcgtttc cttttttctt tttgttgtgc
    27241 gctgtatacc ttgaggatat tcactggtgg cgtacactta aatagtaata tattgtgtta
    27301 ctttatgtct gtggtaatag ttatagttgc ttaccaatgt atgttcattt ttcgaaatgt
    27361 tgacttcata atccttggct taatgtttga atttttttgg tgatagattt tttcagccgt
    27421 ataaaataaa tcgtaattct attatagaga gttgt
//
LOCUS       DAYYBG010000026        26584 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000026 DAYYBG010000000
VERSION     DAYYBG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.026"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..1920
                     /locus_tag="ACHAUN_001144"
     CDS             226..1920
                     /locus_tag="ACHAUN_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFM8459861.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            2608..4485
                     /gene="ltrA"
                     /locus_tag="ACHAUN_001145"
     CDS             2608..4485
                     /gene="ltrA"
                     /locus_tag="ACHAUN_001145"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8459862.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            4616..4993
                     /locus_tag="ACHAUN_001146"
                     /pseudo
     CDS             4616..4993
                     /locus_tag="ACHAUN_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            4993..6198
                     /locus_tag="ACHAUN_001147"
     CDS             4993..6198
                     /locus_tag="ACHAUN_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8459863.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            6214..6858
                     /locus_tag="ACHAUN_001148"
     CDS             6214..6858
                     /locus_tag="ACHAUN_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459864.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            6869..7675
                     /locus_tag="ACHAUN_001149"
     CDS             6869..7675
                     /locus_tag="ACHAUN_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459865.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            7697..7927
                     /locus_tag="ACHAUN_001150"
     CDS             7697..7927
                     /locus_tag="ACHAUN_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459866.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            8473..8748
                     /locus_tag="ACHAUN_001151"
     CDS             8473..8748
                     /locus_tag="ACHAUN_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459867.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            8790..9212
                     /locus_tag="ACHAUN_001152"
     CDS             8790..9212
                     /locus_tag="ACHAUN_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459868.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            9231..10823
                     /gene="mobP2"
                     /locus_tag="ACHAUN_001153"
     CDS             9231..10823
                     /gene="mobP2"
                     /locus_tag="ACHAUN_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFM8459869.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            10835..11170
                     /locus_tag="ACHAUN_001154"
     CDS             10835..11170
                     /locus_tag="ACHAUN_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459870.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            11317..11511
                     /locus_tag="ACHAUN_001155"
     CDS             11317..11511
                     /locus_tag="ACHAUN_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459871.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            11543..13915
                     /locus_tag="ACHAUN_001156"
     CDS             11543..13915
                     /locus_tag="ACHAUN_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8459872.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            13976..15436
                     /locus_tag="ACHAUN_001157"
     CDS             13976..15436
                     /locus_tag="ACHAUN_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8459873.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            15447..17651
                     /gene="topB"
                     /locus_tag="ACHAUN_001158"
     CDS             15447..17651
                     /gene="topB"
                     /locus_tag="ACHAUN_001158"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8459874.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            17667..17816
                     /locus_tag="ACHAUN_001159"
     CDS             17667..17816
                     /locus_tag="ACHAUN_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459875.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            17890..18483
                     /locus_tag="ACHAUN_001160"
     CDS             17890..18483
                     /locus_tag="ACHAUN_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8459876.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            18499..19398
                     /locus_tag="ACHAUN_001161"
     CDS             18499..19398
                     /locus_tag="ACHAUN_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8459877.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            19401..19886
                     /gene="ssb"
                     /locus_tag="ACHAUN_001162"
     CDS             19401..19886
                     /gene="ssb"
                     /locus_tag="ACHAUN_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8459878.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            20252..20512
                     /locus_tag="ACHAUN_001163"
     CDS             20252..20512
                     /locus_tag="ACHAUN_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459879.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            20953..21159
                     /locus_tag="ACHAUN_001164"
     CDS             20953..21159
                     /locus_tag="ACHAUN_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8459880.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            21159..21410
                     /locus_tag="ACHAUN_001165"
     CDS             21159..21410
                     /locus_tag="ACHAUN_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459881.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            21425..21862
                     /locus_tag="ACHAUN_001166"
     CDS             21425..21862
                     /locus_tag="ACHAUN_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459882.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            21855..22562
                     /locus_tag="ACHAUN_001167"
     CDS             21855..22562
                     /locus_tag="ACHAUN_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459883.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            22727..22927
                     /locus_tag="ACHAUN_001168"
     CDS             22727..22927
                     /locus_tag="ACHAUN_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459884.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            22943..23122
                     /locus_tag="ACHAUN_001169"
     CDS             22943..23122
                     /locus_tag="ACHAUN_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459885.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            23463..23726
                     /locus_tag="ACHAUN_001170"
     CDS             23463..23726
                     /locus_tag="ACHAUN_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8459886.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            23720..24070
                     /locus_tag="ACHAUN_001171"
     CDS             23720..24070
                     /locus_tag="ACHAUN_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8459887.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(24094..24708)
                     /locus_tag="ACHAUN_001172"
     CDS             complement(24094..24708)
                     /locus_tag="ACHAUN_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8459888.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            25022..25444
                     /locus_tag="ACHAUN_001173"
     CDS             25022..25444
                     /locus_tag="ACHAUN_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459889.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            25454..25657
                     /locus_tag="ACHAUN_001174"
     CDS             25454..25657
                     /locus_tag="ACHAUN_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459890.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            25868..26452
                     /locus_tag="ACHAUN_001175"
     CDS             25868..26452
                     /locus_tag="ACHAUN_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8459891.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         9884 a         4239 c         5457 g         7004 t
ORIGIN      
        1 ccactctaca aaaaagaatt gatcaagatt ctgtatcgaa tgaacaatat gaatgatgag
       61 taggaatgga gaagagagaa tgaaatatcc gttgatatgg ttagaaaaag atgttccgaa
      121 ggaagagacc actagaaaat ttcctcacac tttaagcctg ggtaagttgg gaaaagggca
      181 atcttttaac caagtaaaga atcaaacaag aagaggtgac tgtacatgtt agaagcaatt
      241 aaaacagctt tttcaccaca aaatgatcaa aaagtacttg atccattaaa gcaactagga
      301 aatcgggaaa tagaaattgt aaaatccaaa gggtatgatt tagatttttt aaaacaagtg
      361 atgccacaag gtgggttaaa ttttgatgaa gaatatgtga cgtatggtaa tggttattca
      421 cgatgtgtga cagtttatca ctacacaaaa aatcctacgc ttttttggtt ggccaatctc
      481 atgaacaatc ctcatacagt ggccacactc gatgttagca cagatgaaaa gcaaaaagtg
      541 attgatcaaa tcaatcgtgc cttagatgaa ttaaatgatc gatcagataa tgaacgaaaa
      601 gcgactgacc gaaataaaag cgcggaagaa tacctggaac tagcggaata tgctagaaaa
      661 ttgacttcta atggggaaat ttctaagcaa gtcaaagtga gagtacatgt ttacggaaac
      721 acgattcaag aagtagatga acgagcgcag gaactgatcg aaaatctcaa agggatggat
      781 tatcgtgcgg tgacttatct gcataaaacc aaacgagatt ggcaatcgat gtttaccatg
      841 ttcgatgaac aagctgaatg gttcggaaat atcaaagggc aatcactacc atcgttaaac
      901 attggcggag gtttcccgtt ccaccaccaa acgctaaaag atccaggagg gacacaatat
      961 ggggaaacca tgacaggcgg cccatttgtc tggaatgcga cgtataaaaa tagtgtacgg
     1021 ttgtctacta ataccttgct tctaggaatg atgggagctg gtaaatcgac cgttcttaaa
     1081 atgatcgcgg aagcccattt agctgcagga gactttattt ggggctttga aaagggaaaa
     1141 gactttattc ctttcttaaa ggaatataac ggtattatgg tgcgtttaga cggatctgac
     1201 ggaatgatca atcctttaga gatatttgcc acacggacgt atgatgaagc atccagttta
     1261 tatgacgatg gatcagtcaa agatttgaag atcaatgaag cagcgtccta tcaaacacac
     1321 ttagacaaag tggtctatca ggtacagtta gtgagtcctc agctaaaagg aacgatgaaa
     1381 gcagaattta agacctactt aaatcgtttt tatgaagagt atggaacagt accacgcggc
     1441 tttacttcct caaattcgcg ttcaaatact gatacgcaag tcacagggaa agatccagaa
     1501 gcttatccga cgttcaaaga atttttagac tttttaggac aactggaatt accaggtgct
     1561 tcccaagaga aaaagaatcg aaaagaagaa atggaatcca tcgtagaatc cttatgtgag
     1621 acgtatggca tgatttttga tggtcatagt acgattcgac accttgacca acaacagttg
     1681 gtcttttttg acattgactc tattacaggc ttagatcgag gggtacaaca atgtcaaatg
     1741 tacatggcta tgacgttgat ttggaatcag gcattgattc aaggaagaag acaacgacgt
     1801 ttaattaaag aaaaacaaat ggaagaacaa gacttcaaac gcttcttggc gatgattgat
     1861 gagtgccata atggtgcgac ctgttctgca gtgaaaagct gtgggttgca agtagtctaa
     1921 aaactattgt aataactctt ataacgagaa gtcaaatgag tgggtaacgc cacaaagggc
     1981 ttccactgat actccgacat gcaacaagta gtttatgcag accaacgttg tgtgaagctc
     2041 ggtgaagtcg gctgaaggat accgtaaggt aaaagaaata gtgaatacca cgaaagttct
     2101 ctagaggtaa agggtgtatc cgataagtcg ggtgtccaaa aaaacttaat atggtgagta
     2161 tgtacaaacg tactgacgaa ctagcgaatg tactgtccga aaggcatagc tcctagaaat
     2221 gggagagaca gtgagcaaaa aatcactcat tgtgctaact tgtggttgag taaaagtcgt
     2281 tgttatgaaa gaccatgctg cgttacaggc gtaggagaag ttagggggca aaagctagca
     2341 tctaagttaa ggcatgtgga tagatataag ggaacagggg aagccatcaa catggagagt
     2401 gcatactcga tgaagtggta gtaagaaata tgtgaatgac aaataactta ctttagtgat
     2461 ggtgaaagta gagtcacgac tgatgaaggt tctgtaatgg aactggagga acaggctcga
     2521 ttcgtctgta aaagtcatga aacccgttca accatgggat tctcgtatga ctaaaaaggt
     2581 cacgagccaa ttggagcgtg ataaaccatg acaaaagaaa caagtccaaa agataggaag
     2641 ttgcggcatg cagaatatta tggcatgacg gaaatcttcg ataagatata ctccaaaagt
     2701 ttgaagggag ataatttcaa gaatctaatg cagattatca tttctgataa caacattgtc
     2761 ctagcatatc gtaatatcaa gagaaatcac ggtagtgtga caagaggaga agatggagta
     2821 acgattaaag atattgaaaa tctcaaccgt aatgaattcg tagaaattgt taggaagcga
     2881 ttcgcacatt ataaccctcg gaaagtaagg cgtgtagaaa ttcctaaata taatggtaaa
     2941 ttaagaccgt tgggtattcc atcaatatgg gatagaatcg cgcagcaatg catcttacaa
     3001 gtattagaac caatatgtga agcaaaattc aataatcaca gctatggttt ccgcccaaat
     3061 agatcagcag aacacgcaat agcagattgt atggcccgaa tgaatcaaag tcacatggaa
     3121 tatgtggtag atgtagatat caaaggtttc ttcgacgaag tcaatcatac taaactcatg
     3181 agacaaatct ggtctatggg tatacgagac aaacagcttc tcttaatcat taggaaaatg
     3241 ttaagagcac ccgtagtact tccaaatggg aagttgatat acccaacaaa aggaacacca
     3301 caaggcggaa tacttagccc attacttgcg aacatcaatc tcaacgaatt tgactggtgg
     3361 atagcaaatc agtgggagga aaaagatgcc aaagagctaa aacatcattt taggaaaaat
     3421 ggagcttggg ataaaggtaa tatctatcac aagctcagaa agagtacaac tctaaaagaa
     3481 atatacctag tgagatacgc agatgatttt aaaattttct gtagaaatta taatgaagcg
     3541 aaaaaagttt tctatgcaac taaaatgtgg ttacaggagc gtctaaagtt accaatatct
     3601 gaagaaaagt caaaaatcac aaatctttca aaagaagaaa gtgaattttt aggctttacg
     3661 atgaaattag tgaccaaagg taacaaaaag gttgttaata cgcatatagg tacaaaagca
     3721 caaagcatga ttaaagtgaa actaaaaaaa caaatcaaac gaattcaaaa aagtcctaat
     3781 agcaaagata cctttatcta tattggagtc tataacagta tggttattgg gattcataat
     3841 tacttcaaaa ttgcgactca ttgtagtgta gcaatgcgaa aaattgcaag agaactcaga
     3901 ccaagttttg aaatacgact acgagaacaa ggaatctcaa aagaaggaca atacggaaag
     3961 tacgaaggat acaagcctta cctacggtca ggacaaatac gatatataag agggtttcca
     4021 atattaccag tagggtatat tcaacacaga catccgatgt gtaaaaaaag aaatattaat
     4081 aaatacaccg aagaaggtag ggaagccatt cataagaagc aacaaaccgt agaagaatgg
     4141 aagatatcgt ggttgtgtag caatccagtg cttagtaaga gagcaacgat tgaatttgcg
     4201 gacaatcgaa tttcaaaatt catcgctcaa aagggaagat gtgcagtaac aggtgaggag
     4261 cttatcttat ctgaaatgca ttgtcatcat attatccctt atcatgaaag caaaagtgat
     4321 agctatgaaa acctagtgat tgttactgag gaggttcatc gggtaataca cgctacacaa
     4381 agtgaaacga ttgaggaact tctaaaatat ctaaaattaa atccaaaaca aaaagaaaaa
     4441 ctcaatgagt tgagattaaa agttggtaac gaagaaatat cttaataaat atttgtagga
     4501 cgggaaaaca gaatgtcttt tatagacgag ggaacgccgt atgcggtgaa agtcgcacgt
     4561 acggtgtgga ctgggggaaa agacggagat aatatcaaac tcttacctat cagtatcgat
     4621 taatccagat tttattgaaa cggtaaatta tgtcacaaac tttcaacgag aaatgcgtaa
     4681 agtagaagca atgactgttt tagctacaca atccccacaa gaaatggtgc cagaaaatat
     4741 gtcttctgat aacttctcga aactgaagaa agtctttgaa ttttcttcta cgaagatctt
     4801 tatgcgtatg gatgaatcta ttttacgtca tatagaagga ttagtgggta aatcaatgac
     4861 gaactcagaa ttagaatcca ttcctcaaca aaatcaagga gacgctgtga tcaattttgg
     4921 ctcaaaagaa accattcgtg ttcattttag tccgaacaaa cgtcaattaa aattgtttga
     4981 tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca agcagtatgt taagcgtcgt
     5041 gcaaagcggc gcttattttt gtggttgttt ggtacttctg caggtttgat tactattttg
     5101 atcactgtgt tcgtgaccct atttctaata cttgcggcgg gctcgattga taattcggat
     5161 tcagattcaa gcagtggtgg cgaagctttt acaggagaat attcggaagg cttaccgatt
     5221 tataaagaaa tcaaaggaag aggccctttt tctgatgaga ttgcgcaata tgccgtaggt
     5281 gcagcagtga aatataaatt attaccaagt gtgatccttt cgcaatacgg ttacgaatca
     5341 gcgtttggta catcggcgtc cgcaagaaat gatctcaact attttgggat tacttggttt
     5401 gatggttgtc tgtttcctaa aggaactgca cgaggaattg gtggcattga aggtggctgg
     5461 tacatgaagt ttcccaactc aaaagctgca ttttcctatt acggctttat ggtagccaca
     5521 caatccaatt ttaatgcttg tgtaggaaat aaaagccctg gcgcaagctt attgatctta
     5581 ggacgaggcg gttatgccgc tgcaggaatt acggaagata gtccgtatta tacaggttgt
     5641 atgtcaatta tcacttctaa taaattaacc gaatatgatg aattcgccat caaacattgg
     5701 ggtgaaggtg gcaataacaa cggcacgatt actggtgaat ggacgaatcc attccctggc
     5761 agcagtttag ataaaagttc cttcagcggt ggtcaattat ttggaacgaa tccaggcggt
     5821 gaattccgtc cgaacggctt ccatgatgga ttagattttg gttcagttga tcatccagga
     5881 agtgagattc atgcggttca tggcggaaaa gtcgtgtacg ttggcaatcc tggtatttca
     5941 ggtttgggtg cgtgtgtgat cgtgattaat tacgatggct tgaatatggt gtatcaagaa
     6001 tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg atcaagtgaa agttggtcaa
     6061 gtaattggga tacgagatac agcacacttg catttaggat ttactcgaat ggattggcgt
     6121 caagcacaag gacatgcatt cattgatgat ggtacatgga tcgatccgtt accatttttg
     6181 aatagcagta aaaaatagat caggagaaaa aatatggaaa ataaaacgtt aaaaatcgtc
     6241 ttaacagctg caattagcgt tagtttaatc ggcaatattg cgttgttttg gcagctgaaa
     6301 gaggaacaag cagcaaatac ctataaaatt gaagaagtgg ctaaaaacgc gtctgagaag
     6361 ctcaaaaaaa attcctctga ggcacaggcg aagatcgaca agttaaatca aaccatcaat
     6421 gagaaaaacc aagaaatcga tcagctgaag caacaagatg gcacaaaagc aaaagaggaa
     6481 gtaactgaag cacaaagaaa gacggcagaa gagttcggtc aattggccat tgataaatca
     6541 ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg caacccaaga agtgatcgac
     6601 aaattaagtc ctacagacga tgatcaccaa catgatgatt acggaagtta ctcctttaca
     6661 ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa aagaggaaca aaattttgtc
     6721 gtatttgttg attatacaat cggcaatccg caattcaaag atgtcaaacc acaaaaaata
     6781 aaaggtggat taactgtcac tgaaaaacaa gtagatggtc aatggaaagt catagacttt
     6841 agttatttca ctcgttagga ggaagaaaat gagtaagaaa aaaggagaaa gacaacgttt
     6901 caaaaaacga aaaagttatc ttagatggct gggaatcttt atgattctcg gctttttgat
     6961 tcttgctagt ccgatttata cgaatcagaa ggtagatata ccagatgagg taagcacgaa
     7021 cgccttttta aacacgaaag agctgacaat ggtttcaaaa gaaaaaggag cgaaccagca
     7081 attagttttt gaatttgcga tcgatgatga agatcaaagc ttgttaaaag aactggctaa
     7141 tcttaattat cgcgtagaag taaagacagc taaaggggat tatcaagcaa tacgagccaa
     7201 agtgattaag gtgtctgatg attattttgc agtgaagcta acgcatgtac cagaagaata
     7261 tatcgccatg cgtttaacca ttattcctga aaaaattgat ccaaaagtgg atatgcaaga
     7321 accacaagat ctgatttttt atattcacga agataaggtc aaagatcacg tgaaagacga
     7381 agattatgaa caacatgcga tcaattacaa agtgaaagga tacaagaaag aacaaaaagc
     7441 agctcaaaaa caaatggaat ctttccaagc aaccattgat ttgaatgaag aattagtgaa
     7501 gaagctaaaa gatcaattgc cttatcaagt cgcagatgat caggaaaata ctgaaaacaa
     7561 gattaatagc taccagcaag aaattgaaac atcgaaaacg caactgaaag atcaagaaga
     7621 gatcattcaa aaacttcaag aaaaaattga tcgtatgaca aaagaaaaga tttagaagga
     7681 caggtggagt gaaaaaatga agcgaacaaa cttggctgac aaagatactt tcgctgagca
     7741 gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat gtcgagaagt
     7801 gaatttatct aacacaacaa tatctgagtt gaagaatggt cgaaagaatt tagatacctt
     7861 aaaattcaga tcatttgaag ctctatatgt agctgctaaa aaatatttta cagctgataa
     7921 caaataaaaa acaggagtat tttatgtgaa aaaagtatat aaaaaatgct tatgaatcag
     7981 ctatatcacc ttaaccattt gttaatgaga tgggagaaaa cattaaccac gattctaacg
     8041 caaacatcaa gcacataaaa catttaacac tcctcctttc tggctgaaga tttcaaccaa
     8101 acaggaagac agcgtatcaa ttatagcata cactttttct taaattcatt tttcatatga
     8161 caaaatattt ccttctaatc gacaaagtta gaaggatttt ttattgtcta aatacacaaa
     8221 taaccttaat ttttaaagag cataaggaaa cgtgatatca ctttctcacg aaaggtgtta
     8281 ggtaggctgt gcctaccacg tttctaagaa agataaatga tatcaaaatt ttggatttgt
     8341 gtgtagattt gtgggtagga tgaaagcgaa tgtgggcagt aaaataagcg tttgtgtgta
     8401 gcaaaatcaa aaaatgtgta gggatatgtg tagctacaca tatccctaca cataataaat
     8461 ttggaggaaa atatggactt attaattaga aatattgaag aaaagtatat taataaaatt
     8521 gacaaacgtt gtgaagaact tacggtcaag actggcaaaa aatggagtcg aaatcaatac
     8581 ctaaaagttt tgatcgaaaa tgattttgat cacgctcttc tgaattataa aaaagaccaa
     8641 tttgatcggt tgttagaaaa atttgtggat attcaaactt ataatacgaa aactttagaa
     8701 gaatatattg caacaaataa tcaattaatt ggtttgttaa ttgaataata aaaatagtat
     8761 tgtgaattta taaaaggagg aaatcaattt tggcaaaagc aacaagaaaa catgtatatc
     8821 tttacgatat gcatatcgaa gtacttgatg aaataatttc tactcatgaa gatatatcta
     8881 attatagtga agcagtcaga catttgtgta tgatgtatga tccaaaaatc gaagaaaaaa
     8941 taaatccaat tcaaattaaa ttgaatgcaa tgggaaaaga aatttctatt ttaaccgaaa
     9001 tggtttcaga gtttgcggat cttcatttga aagatacagg aatactaatt ggtacaaatt
     9061 caactgttta tcaagaagca gaagaacgtg tggaaggaaa aattaaatcc aatcaaacca
     9121 aaaaatattc ttctaagtta aagaaaccag tgaatgaatc tgttcatacc agagtagatc
     9181 catttgatcc aacgaagatt ctaaaaagat aaaagtagaa ggagaacgtg atgaataaat
     9241 cgcctgcagt tattcttact tgtcaattta ccttgcccaa taaaaaatct ttttcaacat
     9301 acattgatta tatgacaaga gaaaaagcac tggaagaaat ggaacgacgt acgcccgaag
     9361 aagaacagga actgcaacga atcaagaatg ctttagagga tttttatatt cctgaaggag
     9421 aaactttttc ggaaagtaag acggataaag aattcagtga aacgtcgaat gaagctcaat
     9481 cgttgatgga acatggaatc aattttgaca agttagagga acaagatttt accaaatatc
     9541 tttcttatat gacccgtcac tatgcgttag aacaaaagaa agaactgacg caaagggaac
     9601 aaaaagaata ccaacggttg aaccaagcaa tgaaaaaata tcaaacagag ccacataaaa
     9661 aagcttcagg aaatcttcct ggagtttttt ctatggcttc agacgaagtg aaaggaagtg
     9721 atctgaagga gatcaaacaa atcttccaaa gaggacaaca aaaaggctca atcatttatc
     9781 aagatgttat ctctcacgac aattcgtatc ttgaaaaaat gggcttgtac gatcccaaaa
     9841 cagatacctt agatgaagaa ggtttgaaag cagctggaag aaaaatgatg gagacattat
     9901 tcgagaaaga acaattgaat gaaacaggtt actggatggc aacgattcac cgtaacacta
     9961 agcatatcca tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa ccgtatactg
    10021 agattgaaaa tggtgtgagt tatatcgtac cgaaggggaa gagaagacaa gcaacattag
    10081 acgcaatgaa aaccaattat gtacatgagt tagaacgttt ctcttatgaa aaggatcgaa
    10141 tgatcggtat ggagaaacga aatttattga cacgaaaaag tgatttgcgt aacaccttaa
    10201 caaaagtagt aaaagaacca gcacgttacg atcgacacgc catgaactta ttaaatgaag
    10261 tatataaaac cctacctgaa aaacgttcgg aatggaatta tggagatgaa aatcgaacca
    10321 agctttcacc atttacaagg ggaaaacttg atgagttaac caaatatgtt ttaacaaaag
    10381 aacctggata tcaagaatat atacagattt caaaagcatt gaaagaagaa gccaaaagcc
    10441 tttatgggga aagcaaaaga gaaacaaaga atgcagaaaa aaacgccctc tttgatttaa
    10501 aaaagcgcac aggaaatgcg attttaacag aactgaagag acaggatagt caaataaaac
    10561 cattaataga aagtgtcaat cagatgcaat ctatttacaa tgaacgatcc tttcgaacac
    10621 tcaaacagtt gccaacaaat gaatacaagt atagttatca agaattcgta aagcgtagga
    10681 aacaattcca acaaagagtt gtcacgcagc gaagcttgcg tcaattaaat cgaacaatta
    10741 atgcacatga agaaaaatac cacgcaagta aagcatatga agagcaacag cgaagaatcc
    10801 aaatggaaca agaaagacaa taggaggaag agaaatgaaa caatatttag atcaatggaa
    10861 agtcatagaa ggatcactaa aagaagaacg aatcgaacaa cttcctgatt gtttagaaaa
    10921 agagcatttg tttcagataa gcgaaatgtt gagaaacgaa cagtttgatc caaatcaatt
    10981 tttagttgtt gagtacccag ctactggtgt gtattgttgt aatcatgtga agggtgaaaa
    11041 atattttatt attcaagaat acgaaggtaa gttagcgcct tattatacta cttgggaaat
    11101 aaatgaagag ggaatcaata atttcccatg tgaaagcatt gaagagtcta tttctctcac
    11161 ggaatgttaa ataaatagag acgtaaaaga gtcctgaacg tgattgtcca ggactctttt
    11221 tcaatgaatt gttttccgcc aattcactga gtggtgcgat tcaattttat tatatcaaaa
    11281 gaaagtgaga ttccaaagtg aagggaggaa gaagaaatga ctcagacaac gattggtgtt
    11341 gtggttgaaa aaagcaggga taatctcgtt tttgtgacag aaattcaaac aggtagagct
    11401 tttgtagtca cagacaaagc tgcaaaagcg tatcaaaatg gcaatatttt aacgttaaat
    11461 ctgactacga agatgttcgt tgatgcagta gaaaattatc catttgttta gacaaacgat
    11521 attttacaag gaggaaacgg acatggcaga agaaaaagaa ccactagata atagtcggtt
    11581 gggaaagtcc aaaagaaaat tagtacggtt gcaaaacgaa ttaaataatc aatttcaaaa
    11641 gctatttgat caccaaaaat tgacaaatgg gcaaccaatg aatgacaaac gaaatggtcg
    11701 ttcatggttt aaccagcaag aaaaaataga aaataaaata caaagtttga gagaagagat
    11761 caagcaacaa gaaaaacagg tagaaaaact tgaaagacaa gaagagctaa aggaaatggg
    11821 ctataacaag tacggcggac tggatatgac gatcgataat attccaagaa tcaaagaaga
    11881 aatcgaacgc tttgaaaaag gtgaatcgac tttttcagcc gccacgatcc gaaaatatca
    11941 acggaagttg gaaacattag aacagctgaa agaaaaatca gaaaaaggaa aagaaaatat
    12001 cctccctgaa gtacaagcga tcattgacag cggacgagtg acgcaatgga aaaaaaatcc
    12061 aaccatttat tttttgaaag gctatcgaaa agttgcttta gaactgaatg aaaacggggg
    12121 atttgaggaa tcaaaaaaat accaagccac gtcggaagag gaaaaagcag ctgttcgtga
    12181 attacttgaa gaggctgcac caactgattc tctgaaggag aaagaaaaag atcatttcta
    12241 tcgagtagaa ttcaacgaaa atgatccaga aaaaggattg aaaagttatg gtggggagat
    12301 cgtaacatta gaattgatcg atcaacttaa aacgttggat aaccaatacg ctgcagaagc
    12361 aggttattat aaattctatt ttgaagaagt gaagaacgga gaagtagtag atcagatccg
    12421 aatggatcta ggggatggcg tcaacagtaa tcaccccatc tatttagagt tagcaaaatt
    12481 gtgcgaacaa gtacctgaga aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa
    12541 agaacaaatg cagtcgaaag aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc
    12601 agaagagatc aaacaagaag ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa
    12661 gcatacccat tcttctgaag acgtcaaaga acttttgaac tttatgtctc gttttccaga
    12721 acgatccttt agaaatcaat tattgattga ggaacaattt ccaggtgcaa cggcttgttt
    12781 aggtcgtgcc gcgttgaaaa aagaaggaat ctatatcaaa aaaggggaac aagggaacaa
    12841 gatctttgtc agaaaaacgg tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga
    12901 aacagaagct acaccagaag ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa
    12961 aaaaccttac tacacgattg aaaaagtgtt tgatatcaca caaacacagt taaaacctga
    13021 agactatccg aaaatctttc cgaaccgtgt ctttgatttt caattggata aaaaaggaca
    13081 gtcagaactt caagctggga ttcaagcggt agccaacaat attggaatac aaattcgaga
    13141 tatgacagaa agtgaagtct atcaaagaga attaggacaa gcacgtgggg cgtatgttcg
    13201 taacgaattt actggcgaag aagaaatcgt catgaatact cgaaatactc ctacgcagca
    13261 tttagctaca agtattcatg aattagccca tgcaagaatg cacaaatttt cagaattaga
    13321 taccgcaact aaagaattgc aggcagaaat gacttcttat attgtgtgca aacattttgg
    13381 aatggacact tcggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac
    13441 gttggatgat aaggaagcgg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc
    13501 aaatgaattc attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga
    13561 gatccaacca gcagaagtga agcaggagca taaaaatact gtagaaacgg cagagaataa
    13621 gaatattgtg cccgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga
    13681 agatacgggt cgaacgttac atttgaagga tgcggcgttt attgaagatc cgcagcataa
    13741 tcaagtgatt ttaaaaggaa aaagttatgg aaaagagatt caaacggcgt taccttcaga
    13801 agaatttgcg ctaggaaatc cattccgatt ttcacttgaa acagtgtcag aagtggccaa
    13861 acgaaaaaat gaaatctatt atgaacaaca attgtcacaa aaagaacgcg tataaaacaa
    13921 gtaaaaaaga ctatctgaaa aacggatagt cttttttctt ttgggaggtt tgaaaatgac
    13981 cgaaaaaaaa atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg
    14041 aaacaatgga atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc
    14101 atttgttttt gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga
    14161 cattattgaa ttagctaaac tttttttcat agacaaagag atccaagact ctaagcaaca
    14221 atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac gagtggaaaa
    14281 tcttcatttt gaaacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga
    14341 aaaaggaagg aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc
    14401 agaaaaagaa gggttgattg ccgaactaaa gccaaaatat gaacggcaaa atttcttagt
    14461 tcgagacgat cgtttagatc atgcggtcgc ctttttatgg aaagatccac agacaaaaga
    14521 gacagtaggg gcgagttatc aaggaacgtt cattgattat gaacgttttg gtgagagagg
    14581 cacatataag catatagata aaaattcgac agcgaatcat ggctttaacc taaagatcgg
    14641 tgaccctaag cagttaaaat tttttgagag tagtattgat ttacttagct atgctgcact
    14701 gaatcgtgat cagttaaacg atacgtggtt agtctctatg gaagggttaa aacaccatgt
    14761 gatcagtcat tattttggtg aggcagtatc tgaattgaga aagaaacaag cctttcctca
    14821 atccatcgag atttgtgtgg ataatgaccg tgcaggacat attttctacg aaaaagaaca
    14881 attaatgggc gcagtcgatc catttacgaa tcagaaagta cgttgtgaac gaggaatcgc
    14941 taatgattgg caagtgccaa aagaatacaa aatcatttat gaagaagtcg caaaagaaga
    15001 aaaggtcaca ccagaagcta ttatggccat acacaagaca gaaaacaatc tgcaattaac
    15061 gaaccaactg gtttccgctc acaaagtaaa tgcgtttttt ggccagcagc tttctgttaa
    15121 cgattcaata gaagccatta atttgaaaga tatttgtaga gaagtcgcaa aagaattaaa
    15181 agtttgtgaa cgcgtggatg gtacgtacga ttttgatcgt ttttatcaag aaaaagggga
    15241 tattaatgcg cagattcttt tctcttataa agcagaacaa tactacaaag gctataaaaa
    15301 tcatgaacat gaatttatac ctgaagtaaa aaaggactgg aacgatcaat tgaagcatga
    15361 gatccagcaa caagaaatca gaaagcaaaa acgtgccatg ttgtttcaac aaggcagaca
    15421 acaagaaagg gagtgaaatc gtcatgatga aaaccgttat tttagcagaa aagccgtcac
    15481 aagcgaaagc ttatgccgat agcttttcta aagctacccg aaaagatggg tattttgaga
    15541 tccaagatcg attgtttaca ggagaaactg tgatcacgta tggttttggt catttagtcg
    15601 agttagattc gcccgatatg tacgatgaaa aatggaaaca atgggcatta gaacatttgc
    15661 ctattttccc caatcaatac cactatcatg ttcctaaaga taagaaaaag cagtttagtg
    15721 tcgtcaaacg acagctgcag tcagctgata cgattgtgat cgcgactgat agcgatcgtg
    15781 aaggggagct gattgcctgg tcaatcattc aacaagcagg cgccaatcaa ggaaaaacct
    15841 ttaaaagact ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccaacagt
    15901 tacgagacgc agaagagacc tatccaaaat ttgaagaagt acaagcacgt cagatcgccg
    15961 attggttaat tgggatgaac ggtagcccac tgtatagttt actcttgcag caaaaaggca
    16021 taccaggaag tttttcttta ggtagagtcc aaaccccaac actctatatg atttatcaat
    16081 tgcaggaaaa gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gcccaagtaa
    16141 tcgctcaaaa tggcgcattt gatgcgaagc ttgatccaaa tgaaacccaa gcgacacaag
    16201 aagcatttga agcctattta aaagaaaaag gtgttcaatt aggaaaacag ccaggtacga
    16261 tccttcaagt cgaaacagag aaaaaaagtg cggctagccc acgtcttttt tctttatcga
    16321 gccttcagtc taaaatgaat caactcatga aagctagtgc caaagatact ttggaagcca
    16381 tgcaagggtt gtatgaagga aaatatctaa gctatccgcg aacggatacc ccttatatta
    16441 cggaagggga atatgcgtat ctgttggatc acttagacga atacaagcat tttttaaaag
    16501 ccgaagcgat tccgactcct attcatacac cgaatagtcg gtatgtgaat aataagaaag
    16561 tccaggaaca ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac
    16621 aactaagtcc actgcagcag gctatttatg aacaagtgtt aaaaacaacg gtggccatgt
    16681 ttgcggaaaa gtatacctat gaagaaacaa cgatcttgac gcaagttcag cagcttcagt
    16741 taaaggcaat cgaaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga
    16801 gcgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg
    16861 tgacggtgga tctacaagtt cttgaaaaag aaaccaagcc accacagcct tataccgaag
    16921 gtacgttgat cacggcgatg aaaacagctg ggaaaaccgt tgatagtgaa gaagcgcaat
    16981 ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa
    17041 ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag
    17101 ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgagtgcc gaaatgaccg
    17161 ccaaatggga aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc
    17221 tagccaatat ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat
    17281 ccactgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg
    17341 cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg
    17401 tggcagcatg tacgtctgaa gcctgtgatt tcaaactatg gacaacgatt gccaaaaaga
    17461 aacttacagc gacacagctg aaagagatca ttcaaaatag ccgaacaagc caaccagtga
    17521 aagggttaaa aggtcagaag ggttcgtttg aagcaacgat tgttttaaaa gaagacttca
    17581 ctacaggctt tgaattttct gaaaagaaaa aaacgaactt caagaaaaga acacgacgca
    17641 ctacaaaata atggagggtt aatcttatgc gtaatacaac acacaaacaa caagaaatcg
    17701 aaaatcaaaa agccaaaatt cgttcagaac aggaaaaaat gcgtgccagc aaacgaagac
    17761 tgcagcaatt gaagaaccag cagcggattc aaaaagaaat cgaattagat cgataaattc
    17821 ctttcttgtt aatataaaac taacatgtta tagttgtgtt agttttatat taactttagg
    17881 ggaagagtaa tgaaagacaa aatccaatgt atttttgata aacataatgg caacctatcc
    17941 ttgaaggatg ctagaagtga aggattatcg cctatgacgt tagatcgatt agccgaaaag
    18001 gataaaatag aaaaggtagc accaggtttc tataccttaa aggatgcgtt cgtggatgaa
    18061 ttgtatctcg ctcagagtat ctattccaga gggatttttt ctcatgagac tgcgctcgat
    18121 ctttttcaag taggaacata tatccctaaa aagatcaata tgatgtttcc taaaggatac
    18181 aatgtatcaa aggagaaatt agatcagtat gccattcaac cgcattacac taataaagaa
    18241 aattttgacc tgggagtcac agaaacagaa agcttttacg gcaataaaat tttagtctat
    18301 gatttagagc gaacgttatg tgacatgtgg aatccaagat acaaagcaag cgtagaggta
    18361 aaacaagaag cattaaaaga atatatgaca agtaccagta gaaatacaaa taagctaaga
    18421 agatacatgg atattttaaa atcaccaaaa gaaatgactt tgtatatgtt gccactttat
    18481 tgagaggagt aaagatgaat gacaccgaca caatttaaag agaaaacaag aaatttcgca
    18541 gcagagaaac atttaaaccc acaaattgtc caaagatatt atatgatgga aaaattatta
    18601 gaatgtattg ttgaatcgcc ttatcatgat gattttgtat taaaaggggg atttctgatt
    18661 ggatcaaaat acgggttaga aaaccgttca accgttgata ttgatacgac ttatcgaaat
    18721 tcgaaactaa caaaagaaaa gttagaaact gttttttctg aattaacagc taatcctaca
    18781 aaagatggaa ttcgttttga attaaaggga ttaacagaga cgagggaagc cgattattat
    18841 ccaggttttc aagcaaagtt tattgcttat cttgaaaatg cacgtgtacc ttttaaatta
    18901 gatattactt caggtgattc tattattcca gcagcattga tgtatgaaca caaattaatg
    18961 tttgaagata aaacgattca tattccagct tatcctacgg aacaaattct agctgaaaaa
    19021 ttacatgcca cgtttagttt tggaaaagat aattcgcgca tgaaagattt ttatgacctg
    19081 tatatgattc cgaaattaga acgtatcgat gagaaaaatt tgtataagag tgttcaagga
    19141 acattcaagg aaagaaataa tcaatcggtg tttaagcatt tgtatgatca ggaaatgcct
    19201 caattggtga aagatgaggg cttgagagaa aagtggaaaa gataccaaca agaaaactat
    19261 tttgccaaag acatttcttt cgaatctaca ctaacttccg ttgattccct gatgcaatca
    19321 attgtagagc aagagaaggc agaacgaaaa caaaatttta agagatcaca acaaatgaat
    19381 caagaaatgg agagataaag atgatcaata atgtaacttt agtagggcga ttaactaaag
    19441 atccagattt acgttatacc gcaagtggca cagcagtggc gactttttcc ctagccgtta
    19501 atcgtaattt cacgaatcaa aacggcaatc gagaagctga ttttatcaat tgcgtgattt
    19561 ggcgcaaacc agcagaaacg atggcgacat tagctaaaaa aggcagcttg atcggcgttg
    19621 tcggacgaat tcaaacacgg acttatgaca accagcaagg acaacgtgtg tatgtgacag
    19681 aagttgtggc cgataacttc caactattag aaagtaaagc agcgacagaa agccgtatgc
    19741 acgctgatca atcgtccagc agtccaagcc ctaccacctt tgaacaacgg gatacagcga
    19801 cgcctaacaa caatggattg aacgcttcac agaatccatt tggtggacaa tcaatcgata
    19861 ttagcgatga tgatctaccg ttctaagtag cattttctat tggttgcatt tgctacggtt
    19921 tatgttataa tttagacaac aaaaagaatt ttcaatctat aaccaaaaac cactagctgg
    19981 cgggctagtg gctttctttt acgtttctat tataagaatt aacgggtagg cttactgtct
    20041 ttcttggcaa atttacaatc aagccatttg cagatatagt gcgcggctac ttctgccatg
    20101 attgcaaaaa caaaatcaaa aagaattttc aaacctataa cctcctcccc ttgccagatt
    20161 aggccggggt agcagacagc aagactatta tagcataatt gaaatatcca aatgattgga
    20221 tttttgctat aattttagcg aggtgattgt gatgaacaaa tatgttggtt tgcttgataa
    20281 aatccgagta ataaaaacgc aaccgttatt ggttcgcttt actttacaaa cgattcatga
    20341 atcgattaat tgtgtagttg cagatattga aattatcgat aaattgctaa ttatggatga
    20401 tggaaaatac aatattgccg tcacaggtca cttcaataaa cgaaatcagc tagttattgc
    20461 tagcatgtat gttagaaatc ccgatcattt tacgcgatcg atgggcatat aaataaaaga
    20521 agagttgtag tcctaaatga ggattgcagc tctttttgtt ttacatagaa acaggaaaag
    20581 acctttttag gaagacacaa gtgccttctc aattgaaaaa cctttctctc actccaaccg
    20641 ccaccgccgt tcgagaagaa aggaagtgaa cgctgcacgt tcactaagat atggtgtctt
    20701 gcaaagcaag acaaaataga caaaacttcc tctcaccaaa aggttcgttc ctgttcgtta
    20761 tctctttatt gatttgccag accggcaaca agttgccttt tatcaccaaa aagcgacaag
    20821 gacaagggta aatggcacgc tgcgcgccct tgtccttgtc gcttttcgtt gatttattct
    20881 ggccaacaaa tcaataaaga aagaactaac gaaaggaagt aaaacctttc ctaagaggag
    20941 gcagaatcaa acatgcgaaa gaaatttgat tattggggtg ttcccttcag cgaattattc
    21001 ccaaactatc atgcaccaca tactgttgaa tgcgattgtg gagaaagagc gaaatgtatc
    21061 aaaagctatc gtttgtatca atgtccaaca tgcgggaaaa aatatacctt gtcttatggc
    21121 gattacattt tgattgatga gaaaggaaag aaacgctgat gaaaacacaa cacaaattat
    21181 tgactgtaaa agaagtcgtt caaattttgg tggacaaaga agtcatttct gaaaaagtag
    21241 cagaagcttt acttattccg cggtactgga cgatagaaac ccctttagta gaggatgcac
    21301 cgactatgct atttgaagac ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa
    21361 acatggaaac ttatgaaatc gaatattggt taaccaaatt agagaactaa aaaggagaaa
    21421 aaatatggct caaaattact atgacgaatt tgtcaaatta cctttggata aaatggcaca
    21481 aaaaatggag gatatgacgt tcctatacca tgaaacacgt gtacccaaaa aacattacaa
    21541 ggaaaaattg tcagtcgcag tggaagaaat gattgaatca ggtgtcgaaa tgaatctcat
    21601 tgcgacgtac tatcgaacac tggaagaact gaaaaaacaa aatggaaaat ggtttttcca
    21661 agccttacta tgcttggaag taggtgtcaa accaagcact atcaaacctt ccgaatatca
    21721 agccttagaa ctgacttatg caaaattcgc acagattaag aaagctaaag caatggctag
    21781 cgactggcta gatacttttg aaagtatcga aaaaaatggt gcattttata cgattgaatc
    21841 ggaggagaaa gataatgaat aaggtggttt atcagctaac aacagaagat gtttcgcaat
    21901 ttttaacaag caaagaacac ttgaatgaag taatcagacg gattcaaaaa gactttagtt
    21961 gtatggaaga aatgaagtac tttattcaaa gagtggaaga acagttgtat atggaaagcg
    22021 aaacaagtca aacgaaggtc acagtcagcc ataatcattt agacgtgtat aaacgtcgtc
    22081 ctgaaacggt aaaaaaagga acagagcttc tacgactaga aacagaaaaa tacatcatga
    22141 atgtgtacct agaaaggagt gagctggtcg aaaactttgt cagctacacg atttgtagga
    22201 aacactatta ctcagaatca gaatattatt caattttcga gagcatggaa ttaatggaag
    22261 aagaaatgga agagctaaac agcaaaaatg cgacggtaga cgattattac cttgtattta
    22321 ctaggttttt gacgtgtggg acggaatcta tcgtgttaat gggaatggat tatgaactcg
    22381 ctgtacccga agcatttttg agggaatatg tagaagaagt agataaccca ggcgacgact
    22441 ggtacacgac tttaactgat tttttagaca cctacactta cgacgataca gaagaaatcc
    22501 aaaatgaact tgaccgtaaa cgaatgccat atcaacgatt tgaaaggaaa aaaggtaaat
    22561 aaccatttat accgaaaagt gaggatgtgt gaaataagaa caaaaaacct tgaaattaag
    22621 ggaatggttc ttatttcgca ccaatcgctc caaccgcccc cgccgcttag gtggtgcgaa
    22681 ataagcgggc aaagcccgat aacctaagag aaagaaggag atccatatga ataaaatcat
    22741 tcctctattg ttcgtactgt ttgttggcga gatacttatg cgtcatgctg cagctgtaca
    22801 tttaaacttc gtagtattct taggaatact ggtattttgt ttgatctttt ggcgatttgt
    22861 attagccctt gtgagtctac tattcaagat gattgcagcc atatttttca tagggttatt
    22921 tcgatgaaag gagagagtac taatgccaat tgcaatagga aataaacggt tacccgtgac
    22981 attagatgaa aagagacaaa aagaattgca gcaactaaag cagaagtacg gaaaaagtga
    23041 atctaagatt atgtgtattg cgttagatct attgatcgcg caagaaaaag caggatttga
    23101 tataccagca ctaaaaaagt gacgtcactt ttcatttgaa gaaacaaaag tgatagcact
    23161 taattgttcc tactatttag aatattttct atgtcctttt tattccattt tgtatcaata
    23221 actatgaaat ctttaaagta tccagaagag attattttta gatcaatttt tttcttacct
    23281 gattctaaag attttgtttt cttaaacata ttatgatttc ctttcgacta ttttcatata
    23341 aatgttaaca tagtttatgg attcatggaa tgttaatcta atttaataga aaaggataat
    23401 taagtatata cgttttttct tatgtatgtt atactatgta tatacagaaa gaggtgtcta
    23461 ctatggaaat gttacctata aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt
    23521 atattatgga atatcttgga attcatacag aagacaaagt gaagataata caagaagaag
    23581 taaacggtca gaagcggcta attatcgaag cggctaatat agaaaatgaa ttaacaattg
    23641 aacagttatt tgaaaattac actgatgaaa gaaatcatgt gacgattcaa aatttaggtg
    23701 aagcagtagg taatgaaaaa tggtgagaaa acctagacaa ggcgatattc tattattgaa
    23761 tacagcacct cgatctggac acgaacagac aggtaaaaga ccttatattg ttttaagtca
    23821 tgatattatt gcggattata gtaacgttgt tacagttgct ccaatttcta gtacaactag
    23881 aaactatccg ctatatgtaa atataaactc agaatatgaa atgaagacaa caggaaaagt
    23941 attattagat caactaacga caattgatta tgaagccaga gagtgtattt ttttggaaaa
    24001 agcgcatgat agtttagttg aggagttatt gatgaaagtt agaacagttt ttcaaaaagt
    24061 aagcaaataa aatagaaaca gcagcatgat caactattcg tgattgacca tgctgtcttt
    24121 ttttattcta tatacggttt gtctcgtaac attgtagtct tttgcaatct tagcgatcgc
    24181 aacacctttt tttagatcct caacgatgtt tttataaaca agacgacgtt ggggatcttt
    24241 tgcattggca ctgtatagct taggacgtcc tttataaaca ccgttcgctt ttgcaatctt
    24301 aatgccttgt gcctgacgtc gtttagattc ggtacgttct tgttcagcga tcatggccaa
    24361 aatttgaatg atcaaatctt taatgaattt atccaggaga ggattgccaa ttgcttcggc
    24421 catgatcggt agacttgtga tgatcagctg cacatttttc ttcttcaagt aattgactga
    24481 agcgatgatc tcgtcgtaat ttcttccaag tcgatcaata gcttccacaa taaaaatatc
    24541 ttgatctcgg acgaaatcca gtgcttcttg aaaaacaagt cgatgagtaa tcgttgcgcc
    24601 agattgtttc tcaatgaaaa ttctctctgc accgaatttt ttcatttctt caatctgccg
    24661 ttgttcattt tgatcgatgg atgagacacg tatgtatgca actctcaaaa tttttaccta
    24721 ctttctgcaa ctatttaaaa aacaccctaa tcatacacga gaaaaccaga tttttcaatg
    24781 tcgcggtcgt gtctagttag ggtatacctt atatagacaa aaactaatga tggaggaaat
    24841 agtatgtgtc acttttcttt ttaaatggca atattttttg taaataaatg tcaatcaaga
    24901 aaatttaaac gattatgaca ttaaaaatat attttatgaa ttttctagta tataacttga
    24961 tttatctgtt aagatttatt aagaaaacgt tttcttattt ttgatttcga ggtgatagac
    25021 aatgaaaaat aagtttattt tatctttagg tattttaact attggaatta gtgtaactgg
    25081 aactacaatt cctacatttg ctgatagtac aaatgaacca actactgtag tagataataa
    25141 ccaagaatta actcctttag ataggaacgg aatagccatt ggtaacccaa ctaacgactt
    25201 aactgttact gaaaattatt taatagtttc tccattactt gctcgagcag cttctaaaac
    25261 gatacgagtg acaggaacga tgatttttga taaagttggt tggccttcta gacctcctca
    25321 atcacggaat tggaaagaaa aaagaaatgg ttattggtac tatggaaaat tgtatttaca
    25381 aagttataaa tcagctggaa acaattggat tgcgacttat tcaggaacct taactagaaa
    25441 gtagggattt tttatgccag aatttgtgaa cacaccaaat ttttatcaag atagcgatat
    25501 tctaagtaaa attaatttac cgaaagaaga atcagcttta caagttcagc gaactttaca
    25561 ggagacacaa aaaattcaaa aatcaatttc taatctagaa gattctgaag aaaaaagaaa
    25621 aaaaagagac caaaaaacat atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta
    25681 gtcgacattc tttttttcta attgaaagtg ttcataaaat cacccatttc tggacacttt
    25741 ctttttttgc tttatttttc aaaaaaacaa ttcatttcat gttcataaac ccataacaat
    25801 atctatgttc agttaaacta tatggataaa gttatgttac aataaaatta atgaaggagt
    25861 gatttttttg aaaataggat acgcccgggt atctactggt ttacaaaatt tggatttaca
    25921 agaagacagc ctaaataaat ttggttgtga aaaaattttt actgatcata tgagtggggc
    25981 aaaaagtaat cgaccaggat tagaaatggc cattgatttt gttcgctctg gcgatacact
    26041 tgtcgtttgg cgcttagatc gtttaggtag aaatatggaa gatttgattt caatcgtcaa
    26101 tcgactgaat gagcgtggtg tgagttttca tagtattcaa gaaaatatta cgatggacaa
    26161 atcaagttct acaggacagc tcatgtttca tttgtttgcg gcatttgcag aatttgagcg
    26221 taatttgatc ctggaacgat ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt
    26281 tggtggtcgt cctgaaaaat tgtctgaaca agatttggaa ttactgaaga ctttagtgga
    26341 tagcggtaca cctattaaga ctattgcgga acggtggaac gtctcaagaa ccaccattta
    26401 tcgttatttg gcaaaaatgg aggagaaaaa tggaaagaca aattctcatt agtttttgtc
    26461 atttaatgct gaaaattttt gccatttatc tagaaaagtg acaatagtat gagaattttt
    26521 tgtaagtgtc tactgataac ttattctcaa atgttgtaag gattttattc atttgttaat
    26581 atta
//
LOCUS       DAYYBG010000027        26391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000027 DAYYBG010000000
VERSION     DAYYBG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.027"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACHAUN_001176"
     CDS             complement(104..1336)
                     /locus_tag="ACHAUN_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8459892.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACHAUN_001177"
     CDS             1591..2160
                     /locus_tag="ACHAUN_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8459893.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACHAUN_001178"
     CDS             complement(2338..2778)
                     /locus_tag="ACHAUN_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8459894.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACHAUN_001179"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACHAUN_001179"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8459895.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACHAUN_001180"
     CDS             3732..4655
                     /locus_tag="ACHAUN_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8459896.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACHAUN_001181"
     CDS             4731..5870
                     /locus_tag="ACHAUN_001181"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8459897.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACHAUN_001182"
     CDS             5863..6663
                     /locus_tag="ACHAUN_001182"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8459898.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACHAUN_001183"
     CDS             6663..7490
                     /locus_tag="ACHAUN_001183"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8459899.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACHAUN_001184"
     CDS             7495..8202
                     /locus_tag="ACHAUN_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8459900.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACHAUN_001185"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACHAUN_001185"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8459901.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACHAUN_001186"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACHAUN_001186"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8459902.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACHAUN_001187"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACHAUN_001187"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8459903.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACHAUN_001188"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACHAUN_001188"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8459904.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACHAUN_001189"
     CDS             11787..13820
                     /locus_tag="ACHAUN_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459905.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACHAUN_001190"
     CDS             13864..15144
                     /locus_tag="ACHAUN_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8459906.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACHAUN_001191"
     CDS             15354..16160
                     /locus_tag="ACHAUN_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8459907.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACHAUN_001192"
     CDS             16172..17392
                     /locus_tag="ACHAUN_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459908.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACHAUN_001193"
     CDS             17382..18968
                     /locus_tag="ACHAUN_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8459909.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACHAUN_001194"
     CDS             19007..21145
                     /locus_tag="ACHAUN_001194"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8459910.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACHAUN_001195"
     CDS             complement(21513..22496)
                     /locus_tag="ACHAUN_001195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8459911.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACHAUN_001196"
     CDS             22874..24265
                     /locus_tag="ACHAUN_001196"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8459912.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACHAUN_001197"
     CDS             24281..25321
                     /locus_tag="ACHAUN_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8459913.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..>26391
                     /locus_tag="ACHAUN_001198"
     CDS             25340..>26391
                     /locus_tag="ACHAUN_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8459914.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTT"
BASE COUNT         8434 a         4418 c         5374 g         8165 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta ttagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg a
//
LOCUS       DAYYBG010000028        25519 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000028 DAYYBG010000000
VERSION     DAYYBG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.028"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..724
                     /locus_tag="ACHAUN_001199"
     CDS             170..724
                     /locus_tag="ACHAUN_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8459915.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(897..1334)
                     /locus_tag="ACHAUN_001200"
     CDS             complement(897..1334)
                     /locus_tag="ACHAUN_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8459916.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1382..2329
                     /locus_tag="ACHAUN_001201"
                     /pseudo
     CDS             1382..2329
                     /locus_tag="ACHAUN_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2600..3035
                     /locus_tag="ACHAUN_001202"
                     /pseudo
     CDS             2600..3035
                     /locus_tag="ACHAUN_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3051..3884
                     /locus_tag="ACHAUN_001203"
     CDS             3051..3884
                     /locus_tag="ACHAUN_001203"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8459917.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4129..5154
                     /locus_tag="ACHAUN_001204"
     CDS             4129..5154
                     /locus_tag="ACHAUN_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8459918.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5292..5417
                     /locus_tag="ACHAUN_001205"
     CDS             5292..5417
                     /locus_tag="ACHAUN_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459919.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5442..6557
                     /locus_tag="ACHAUN_001206"
                     /pseudo
     CDS             5442..6557
                     /locus_tag="ACHAUN_001206"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6640..7536
                     /locus_tag="ACHAUN_001207"
                     /pseudo
     CDS             6640..7536
                     /locus_tag="ACHAUN_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7552..8376
                     /locus_tag="ACHAUN_001208"
     CDS             7552..8376
                     /locus_tag="ACHAUN_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459920.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8408..9718
                     /locus_tag="ACHAUN_001209"
     CDS             8408..9718
                     /locus_tag="ACHAUN_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8459921.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9866..11125
                     /locus_tag="ACHAUN_001210"
     CDS             9866..11125
                     /locus_tag="ACHAUN_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8459922.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11135..12007
                     /locus_tag="ACHAUN_001211"
     CDS             11135..12007
                     /locus_tag="ACHAUN_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459923.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12019..12849
                     /locus_tag="ACHAUN_001212"
     CDS             12019..12849
                     /locus_tag="ACHAUN_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8459924.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12889..15072
                     /locus_tag="ACHAUN_001213"
     CDS             12889..15072
                     /locus_tag="ACHAUN_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8459925.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15133..16353
                     /locus_tag="ACHAUN_001214"
     CDS             15133..16353
                     /locus_tag="ACHAUN_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8459926.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16350..17810
                     /gene="gtfA"
                     /locus_tag="ACHAUN_001215"
     CDS             16350..17810
                     /gene="gtfA"
                     /locus_tag="ACHAUN_001215"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8459927.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17887..18993
                     /locus_tag="ACHAUN_001216"
                     /pseudo
     CDS             17887..18993
                     /locus_tag="ACHAUN_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19315..19524
                     /locus_tag="ACHAUN_001217"
     CDS             19315..19524
                     /locus_tag="ACHAUN_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8459928.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19517..19666
                     /locus_tag="ACHAUN_001218"
     CDS             19517..19666
                     /locus_tag="ACHAUN_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8459929.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19820..20485
                     /locus_tag="ACHAUN_001219"
     CDS             19820..20485
                     /locus_tag="ACHAUN_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8459930.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20769..21854)
                     /gene="xerS"
                     /locus_tag="ACHAUN_001220"
     CDS             complement(20769..21854)
                     /gene="xerS"
                     /locus_tag="ACHAUN_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8459931.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22188..22421
                     /locus_tag="ACHAUN_001221"
     CDS             22188..22421
                     /locus_tag="ACHAUN_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8459932.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22418..22825
                     /locus_tag="ACHAUN_001222"
     CDS             22418..22825
                     /locus_tag="ACHAUN_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8459933.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23962..24480)
                     /locus_tag="ACHAUN_001223"
     CDS             complement(23962..24480)
                     /locus_tag="ACHAUN_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8459934.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            24847..25095
                     /locus_tag="ACHAUN_001224"
     CDS             24847..25095
                     /locus_tag="ACHAUN_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459935.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8687 a         3898 c         4758 g         8176 t
ORIGIN      
        1 tttttgtaca aaaaagtcgg aatattctga gttactgaaa tgatataaaa tcataaacta
       61 aatcccttga ttttattgac tttcaaaagt ttatatatat aagttaatga ttatggtaaa
      121 atatatacat aatcattaac tcagaatatt tttaagaagg gaaacaatag tgagctataa
      181 tgtacaaccg ttaaggacaa atgaagagat acaagatttt cagttttggc taagaagaac
      241 aagtaatccg gaaagagata gttttttatt tttgtttgga atcaataatg ggttacgtat
      301 gtcagatatt ataggattaa aagttggaga tatacgaggc aaaagtaagc ctattattgt
      361 tgaacataaa acaggtaagc gaaaaccgat ttttatagat aatttgagag aagaaatttt
      421 attatatact gaaggtaaag aagaaaatga ttggttattt ccgagtcggc aacaaggtcg
      481 acatataaca agggatcgtg tctatcagat atatgcagat atagcagaaa aattaggacg
      541 agatgatata ggaacacata cattaagaaa aacttttggg tatcactact acaagaaaac
      601 tagagacatt gctaccttaa tgtttatttt taatcatagc tcgcaagcaa ttacaaaaag
      661 gtacattggt ataactgaag atgaaatagg agcatcactt cgtggattta aactaggtgt
      721 ataatttaaa aatttttata aacagtaaag acaaagatta aaagctattt ttatctagaa
      781 cgattttatt tcataaagtt cgcggaataa aagctggggt tactggtact tggtataggt
      841 tcgtgttata taaactaagt aattaaataa catttttagg ttggaaaagg atgaaattaa
      901 aaaaagagaa tcaatacggt ctcaaaaacg caaacgctgc ggaataagaa aaatactgat
      961 aactcaacgt ttagcagact atctaatagg gatagtctgc ttttttcgtt tttgaccata
     1021 cttttgacct tctgcaaact tccttgcttt tcctctgtag aactcatgaa atctgcaaaa
     1081 cgaattgccg tttcctctgt ctgctttttc gcgatatggg tgtatatatc atccatagtg
     1141 gttttaatat ctgtatgtcc aagtcgaaca tacacttctt tgacagacgc accagattca
     1201 aagagcagac tacaatgggt atgacgaaaa ccatgaagtg tgatatgttt taagttattc
     1261 tctttaatga tgattttcaa attgtgattt acatagtcta aataaagggg cttattttct
     1321 tctgatgaaa acaaaagttg ttcaccatct tttataggtt tgattctcat ttttagatat
     1381 tatgcccgca cgtattcatc acatttctat tgtgaatcgc tttattcgac cgacatttga
     1441 cttttatcat aatatactcg gtttaaaatt actaatgaaa acaattaatc aagacgatca
     1501 taccatgtat cacctgtttt tttcagataa tcaccaccgt gtgggaactg aactgacttt
     1561 ttttgaagta caagaaggga acaaccagtt ttttggaacc aatacgattg aacgaacgat
     1621 actaaaagtt ccttccgaag catcattgca tttttgggaa atatattttg aaacacaagg
     1681 cgtttgccat tatggaatcg aatcattttc tggacgacct atcttacgtt ttgaagggcc
     1741 agatgcaaca cgattagctt tagttccctt acgcgaattt gaagatattg atgattattt
     1801 cccgtatgtc acagaccaga tccctacaga gcatgccatt ttaggaattg atgccattca
     1861 actacgcgta caatattccg atgcgactga acgagagtta ttgtcggtag gctggtagca
     1921 caaagaatac gtcagtttct tttcgggtca tcaacgagtg acaatcttag aaaatcaaga
     1981 cactcagttc tatcaagaag ttcagattat tcaagatcgt gagaatccag ttgccattga
     2041 agggattggt agcgtccatc atgttgcgtt tggcgtagaa aataaatctg atttacaacg
     2101 catcgacaaa caattacaag agcgcaattt tataaattca ggaataaaag atcgtgaatt
     2161 ttttgtatca ctttattatc gtgacccaaa tcaattattg attgaaattg ctacagggga
     2221 aggaaaccta gatgcaaaag cgtatgaaaa tcaaagtcca cgctttgaag aaattccttt
     2281 atttttaccg cagtacttaa atttcattcg tgaacaagtg gagcaataat tagctaatca
     2341 aatccatgac gaagagtaag aggttgactc ataagtcaaa aaataaagaa aaacgcatca
     2401 aatccattct aaagaatgtt gatttgatgc ggttcctatt tttataaact atcattttcg
     2461 actaataaaa ctacttatga gtcagcctct ctttttttct tgatttttat acctctttct
     2521 aggtaaacta aacatctgcc aaaaataccg tgtctcattg aaacattctc aactatttca
     2581 aaataaaggg gagtaccaaa tgaacgagta tattgaaatt atccacgcaa gagaaacaac
     2641 ttaaaaatgt cagtctcaaa attcccaaaa ataaaattag tatttttacc agcgtatctg
     2701 gttcaggaaa atcatcgatt gtttttgata ctattaccca agaagctggt cgtcaactga
     2761 atgacaccta tgatagtttt acccgtttat ttttacctaa atacagtcgt ccagaggttg
     2821 atgagattga taatctttct acggcgattg tggtcaatca aaagaagatt ggtggcagtg
     2881 cgcgctcaac gttagggacg attactaaca tcaatgcgtt atttcgtgtg ttgttttcat
     2941 gttttgcaac acctagttta ggatatgcaa atgcttattc atttaatgat cctagtggaa
     3001 tgtgttctgt ctgtcaggga atcggtaaga cgattgcatt tttaatagga ttgctttatt
     3061 tttcggctat tgataatgct gattttgtat tttctgatcc atttaccgca gaagttttgt
     3121 ctggaattga ggaacaggca aagaatcatg gttattttgt attggtgcat aatgttaatt
     3181 cagcagaaga tgttaaaact attcagagaa attggcgttt tgaggggttc attgttgttg
     3241 gtgtacgtgt aaaagaattt ttggaattaa ataacgtttt tgatggtcct gttgtttata
     3301 ttgatacgca tctttcaaaa gagacattaa gagaacttga acaacaaatg aatcgtttat
     3361 ttgtaaatac aaatgattac caagctagta cattggcagt ggaacatttg attcattatg
     3421 gtcatgagaa aatagcgttt ctttcttttg actacattgt aggtgaacca agtgttattc
     3481 aagaacgaca tcaagcttat atcgatacat taaagaaata tcatttaacc tttaaatcgc
     3541 attggatata taacaactct gaatttgagc gaatttatca agaattagct gaatttactg
     3601 caattgttgt tactggagat tatttagcgg ctaaattagt aaaattttta aaaaataaga
     3661 ataaacgagt gatggaacaa ttatcgatta ttggatttga tgatattact tttgcagaat
     3721 taatggatcc tgcgttgaca acaatacgac taaatccagg taacaagggg gcgattgctt
     3781 ttcaccaact gattggactg gccaaacaag aagaaataaa aaatcaagta ctactattag
     3841 agggagaatt gatttctcga gactctgttc acaaaatcaa ataaaaaagc tctgtgaaat
     3901 aagtatagtt aacaataaaa tatccgaacg atagagagaa tgaactgtga tctagcagtt
     3961 cctcgtcgca aattcactag aattagcatt atttactcta aattgttcgg atattttttc
     4021 ttgtttttaa ttatgattac agtgcctatt tttaacgaaa tttgcttatt ttcaaaatgt
     4081 atgattatat gttagtaaac actttcaaat atgaaaagag gagctattat ggtaggaatt
     4141 agggatgttg caaaacgggc gggtgtgtcc attgcaacag tatcgcgcgt gctaaatgag
     4201 gatccgacac tttcagtaac cgatgaaacc aaacgaaaga ttgcagaatc ggttaatttt
     4261 tatcattatg tcaaaaaaag tcccgttgca aaaaatttaa caaatattgc attaataacg
     4321 acagtttctg aagtagatga gttggaagat ccctattttc gaacgattcg cagagggatt
     4381 caagtcgaag cagaacaaag caaagtcaat ttgaagaaaa ttattcgttt gtcagaaaca
     4441 ccgttggagc aagacgattt gctaaaatgt caaggtgtgt tgattattgg gcaggtgcta
     4501 tcttctgtga ttgaagaagt atcagcaatt aattcgaatg tcgtagtgat tgatgaccct
     4561 agtattgaag ctgaagtaga tgcagtttat actgatttaa caaaagcaac gaatcagcat
     4621 ttagatcgat tgtataaaaa aggacaccga aacattgcgt ttatcggagg aggacgtgtt
     4681 cgtttagata gccaaggaaa taaacacgtg tcagatgatg attatcgaaa aatagcctat
     4741 gagacgtgga tgaaacaaaa aggattagaa aaatatagtc aaatttattt agaaggctgg
     4801 accacacttg acggaatgca ggcagcagat ttacttttga aaaaacatgc taaagataag
     4861 ttgccgactg ctatcatggt aggaaatgat cctattgctg tgggggttta tcgttcactt
     4921 caaaaaaatg gtctacatat tccagaagat atttcgattg tgagttttga taatattgaa
     4981 gttgctgaat ttttaacacc tgcattaaat acggtgaatg tgaatacaga agaaattgga
     5041 aaactagcag ttcgcatggt taatgaacga ataaaagaag ttagaaatgt ttcgatcaaa
     5101 gtgattgttt ctaataatat tattgttaga gaaagtgaaa aaaatatcga ttaaaaaagt
     5161 aaacatccaa aaaagggtgt ttactttttt gtttactaat atttttagta aaaaatctcg
     5221 acaaaacatt aagtaaacag ttatggtgtt gttaacaaaa agaaaacgca ttcaaaacag
     5281 gaggaattaa gatgaaacat ttcaaaaaaa tagctttaac agggatagct gttttaactg
     5341 ggacattact cgcagcatgt ggtgggggag atagtaattc taaagacggg caagtaactt
     5401 taagctatgc gagttgagat actgaccaag ctccaggact aagaaaaatg ttagatgaat
     5461 ttgaaaaaca aaatcctgat atcaaagtag aaatggagac aacgccttgg gatcaatatt
     5521 gggttaaatt agaagctgca acaacgggag gtaatatgcc agatgtagta accatgcact
     5581 ctagcgaatc ctataaatat atgtcacaga atgctttgat gggacttgat gatgttatca
     5641 aagaaaatga tattgacatg ggaaacttta ctgaaggaat tgctgatttc tatacgcttg
     5701 aagacgaact atacgcaatc cctaaagatg cttctgtagt ggggctatgg tacaacaaag
     5761 aattatttga taatgcaggt gtcgattatc cagatgaaac atggacatgg gaaacactca
     5821 gacaagctgc aattgattta acagatgaat caaaaggcat ctatggtttt gcatcacata
     5881 ttggaacaga gactggctac tggccattta tttatcaaaa tgggggagaa gtatttgccg
     5941 atgacaatac aaaatcaggt ttgaataccc cagaagcaca agaagcttta gctttttatc
     6001 gagatattgc acttgttgat catgcttcac caacaattac agaattacaa gatcctgata
     6061 agttaagtcg ttttcaatca ggtagattag caatgatgat tgaaggaaac tggtttgcac
     6121 cgggattttt gaaaaatgac tatctagttg aaaatgttgc ttctgccgtg ttacctaagg
     6181 gaaaagttaa tgcaacaatt acaaatggac ttggttgggc tgccagtgcg agtacaaaat
     6241 acccagaaga agtgaaaaaa ctaatggcat ttttagctag tgaggacgcc aataaaatcc
     6301 aagcggaaac gggggcatca atcccagcac taaaaggctt tgaagacgag tgggctgctt
     6361 cttcagatac aattgatttg agtgcttttg gaaaaatgtt ggagtatgga gtccctcgtc
     6421 catacaataa agatggatta cgagctgaac aagcagaagt tcaaattatt aaccgtattc
     6481 tgtctggtga agtaactgtt gaagatggaa ccgctcaaat tgaaaaagaa gtcaatgcga
     6541 tattgagcgg aaattaaatt agttaattat acaatataca ggcgaaagga ttctatttcg
     6601 tctgtatatt aatggatgaa taccaaaaag ggggatatta tgaaaaatcg aatttttgga
     6661 aaatatcgta aagatcaagt gatatggggc tatattttta tttttccaac ggcattagga
     6721 ttaattttat taaatatatg gccagcaatt caaacagcaa ttttaagttt tcaaaaaacg
     6781 gtagggttcg gtgatactga atgggtcgga ttaagaaatt accaaaaaat gtttgcagac
     6841 ggtgaagttt ttcagtcttt attaaacaca ttaatttatg cagtggtttc gatacctagt
     6901 attgttgtat tatccttgtt ggtggcagtt ttaatgaata gaaaagttaa aggattgtcg
     6961 atttatcgga caatttattt tctaccagtc gttgctgcac catccgcagt cgcaatgatt
     7021 tggcgctgga tgttcaataa tgattatgga ctgattaata acatgttggc taaaataggt
     7081 ctcgatggac cagcatggtt aacggatcca tcgattgcga tttattctat tatcattgta
     7141 ggagtttgga gtgctattgg ttataacatg gtgttattac tagccggctt ataagaaatt
     7201 cctaaagatt attatgaagc agcggaaatt gatggtgcta gtccaatcca gcagtttttc
     7261 aacattacgt taccattagt tacaccaaca ttattttttg tagttgtaac aacagttatc
     7321 aatgctttcc aagtatttga tgttattttt atgatgatta ctcctaatag taccgccatg
     7381 tttaaatcac aatcattagc ctatttattc tataaacatt catttatttt aaatgacaag
     7441 ggatatggtt cggcaatcgt tatgttcctt ttagtgatta tattaattat tactgcaatt
     7501 caaatgaagt tgcagaaaaa gtgggtaaac tattaaggag gctatcaaat tatgaaaaat
     7561 aaattttttt caaatgcgcc aatccatatc atgcttattt taggtggtat tgcaatggca
     7621 ttacctttta tttggatgat tttaacttca gggaaaacgt tggttgaatc tacccaaatt
     7681 ccacctacaa ttttccctga gaaatttcag ctaagtaact atcaacaagt acttgattta
     7741 ttaccattca tggcttttta tatcaataca tttttaatgg ttgttggacg agtgattggc
     7801 tcggttttat tttcagcaat ggctgcatat gcttgtgcgc gtctaagttt tccagggaaa
     7861 aatatccttt tcagcttagt acttattcaa atgatgattc ccggttccat ttttattact
     7921 ccacagtatt tattagcaca aaaattgggt attttaaata caattccagc attaatatta
     7981 ccaggggtag ttagtgcgtt tggtacgttt ttattaagac aatttttctt aggtttacct
     8041 gatgaattgg aggaagcggc ctatttagaa ggtgccaata tttggacgat ctttttcaaa
     8101 attatgttgc cgcttgctaa atcaggattg gttgcacttg gaatattcac agcgttgttt
     8161 gcatttaaag atttaatgtg gccattgatt gtcaatactt caattgataa gatgccttta
     8221 gctccgggat tagcaaactt acaaggacaa tatgccacga acttcccaca attaatggca
     8281 ggttcgttat tagcaatttg gccaatagtt gtgatattca tcgttttcca aagaaaattc
     8341 attgaaggaa ttgctacaac aggtagtaaa ttataaaact attaattagt gattggagag
     8401 taagtacatg acaaaaatta catttattgg tgcaggaagt actatttttg caaaaaacgt
     8461 attaggcgat gcgatgttaa ctccaagtct acaagatgca catattgcat tgtacgatat
     8521 tgatgaaaaa cgactaaaag aatcaaattt aatgcttaaa aatattaata aaaattcgaa
     8581 tgaaaatcgt gctgatattg tttcttatta tgatcgcaaa gaagcgttaa gagaggctga
     8641 ttttgttatc aatgctattc aagtgggtgg ttataaacct tcaacagtga ttgattttga
     8701 tattcctaaa aaatatgggt tgcgacaaac tattggtgac accatgggga ttggtgggat
     8761 ttttagaacc ctacggacac taccagtaat gttagacttt gctaaagaaa tggaagaagt
     8821 ttgtccaaat gcttggttat tgaactatac aaatccgatg gcgatgttaa ccatgggtgt
     8881 tttaaaagcg acgagtatta agactgttgg tttatgtcat agcgttcaag tatgcgtacc
     8941 agaattgttt gagcatttag gaatagccga tcaatatgac ttaaatgact ttcaatggaa
     9001 aattgcaggc attaatcata tggcattctt gttggaaatt acaaaaaatg gggaagattt
     9061 ctatccaaca attcgtcagt tagctgcaga aaaaccaaat ccacatccag attctgtacg
     9121 ttttgaattg ttgaaacgtt ttggttacta tattacagaa tcaagtgaac acaatgcaga
     9181 ataccatcca tactttatta aatcaaaata cccagaattg attgacgaat tagggattcc
     9241 gttggatgaa tacttacgtc gttgtgaaca acaaattaat gattgggaaa gcatgcgaga
     9301 ggacattgta aataatgggg agttaacaca tacgagaagc cgtgaatatg cttcatatat
     9361 catggattct attacgacag gaactccgtc agtgattgca ggaaatgtct taaataaagg
     9421 cttaatcaca aacttaccga cagatgtttg tgtggaagta ccttgtttag tcgataaaaa
     9481 tggcgtgcaa ccaacctatg tcggtgaatt gccaacacaa ttagctgctt taaatcggac
     9541 gaatatcaat gttcaagaac tgacagtaga agccgcttta acactagata aatcaaaaat
     9601 ttatcaagca gcgtatttag atccacatct atcatctgaa ttgtctttaa cagatatcaa
     9661 aaatttagtg gatgatttaa tcgaagcaca tggggactat ttacctgaat ataattaaaa
     9721 agaagctgaa agagtaagga attttcaatt ccttactctt ttcttagtta aatttagtaa
     9781 aaatattgac aaaacactta gtaaacagtt acattagtgt taacaaaaag aaaacgcatt
     9841 caaattgtat aaggaggagc ataaaatgaa gtggtctaaa aaaattattt tagcttgtat
     9901 agcactagga gcaacgatct ctttgactgc ttgtggaaaa aatcaggaag atgcaagcgg
     9961 taaggtagaa atagagtttt ttaatcaaaa aaaagaaatg actcaaacga ttcaggagat
    10021 tgctaaagat tttgaagcaa aaaaccctga tattcatgta aaagttgtgg atgtaccgaa
    10081 tgctggtgaa gtaattaaaa cacgtatgct tgcaggcgat gtaccagatg tcattaatct
    10141 ttatcctcaa agtatagaat tgaaagaatg ggcgaaagct ggttatttag aggacttaac
    10201 cgaagaaccg tatttagaga atatcaaaaa tggatatgca caacgttttg ctattgagga
    10261 tcgtgtatat agcataccat taaccgctaa tgtctatggt ttttactata acaaaactgc
    10321 ttttgaggaa atggggattc aagcaccaga aacttgggca gaatttgaaa aaatagtagc
    10381 tgagattaaa gatcaaaaca aagtaccgtt tgcgattgca ggagcagaag gctggacttt
    10441 aaatggttat caccaactag ctatagcgac tgctgctggt ggtgaagagg aagcgaacaa
    10501 tgtttggcgt ttctcagaag ttaatggaat taatgctgaa agcaaagaaa tgcaaaaaga
    10561 ttttaatcga ttagatttgt taagagaacc aaaagctttg caaaacaact ggcaaggtgc
    10621 cggttataac gatacagtag ttacttttac aaaaggtgat gcgttgatta tgccgaatgg
    10681 ttcatgggca atgccgatga ttcattcaca aaatccagat tttgaagtag gaacttttcc
    10741 ttttccagca gatgaagcag gtcaaagtct aacgattggc gcaggagacc tagccttatc
    10801 agtttctgca acttctcaac ataaagaaga agccaaacgt tttgtagaat atatgacaac
    10861 acctgaagcg atgcagaaat attatgatgt agatggtgca ccgtgtgctg ttgagggcgt
    10921 tatcgagaat actcaggatt caccattaag tggtttaact gaacttgctt ttactgatcg
    10981 tcatttagtt tggttagcca aagattggaa tagtgaaaat gatttttata ccttaacaac
    11041 aaattattta cacaatggca atcaaaaagc catgattaac gcattaaatg ctttctttaa
    11101 tccaatgaaa gctgacggaa ggtaggaaaa taaaatgaaa ataaaagaaa ttttaaatcg
    11161 ttactgggca atcatttttg tagcaattcc aattattttg cagatgattt ttttctactt
    11221 tccaatggtt caaggtgctt tttacagttt aactaattgg actggtctga cttataattt
    11281 tgattttgtg ggtttgaaca attataagat tcttttttct gatccgaagt ttatcaaatc
    11341 gattggcttc actttaattg tgacgctttg catgattgct ggacaaattg gttttggtat
    11401 tttgattgcg cgggcgttga acgcaaatat taaagggaaa acattcttta gagcatggtt
    11461 cttttttcca gcggtcttat cgggcgtggt tgtttcttta gtatttaaac aaatttttaa
    11521 ctatggttta ccaaaaattg gtagtatgtt aggcattgaa tttttacaaa ccagtctttt
    11581 aggaacaaca tggggagcga tttttgcagt aatttttgtt ttactttggc aaggaattgc
    11641 catgccagtc atcattttct tagcgggttt tcaaagtatt ccacaagaaa ttattgaagc
    11701 tgctagaatg gatggggcga atgataagca aattttttgg aatattgaaa tcacctactt
    11761 attaccaagt atttcaatgg tatttattat ggcattaaaa ggtggcttaa cagcatttga
    11821 tcaaattttt gctttaactg gtggtggacc aagcgatgcc acaacatctt tggggttact
    11881 tgtttataat tatgctttta aaaacaatca gtttggttat gcgaatgcta ttgctttagt
    11941 cttatttatc ttaattggga ttgtttcact ggcacaatta aaattatcga aaaaatttga
    12001 aatctaggag gtttcccaat gaaaacatca aaagaaaaaa atatttctaa atacttgctt
    12061 ttattattag ggggcttcat gattttagtt cccttagttt caacgctatt cggttcattt
    12121 aaaacaacac ccgatattat gaataatttc tttcgttttc ctaatccagc gactttggaa
    12181 aatttccgaa gattgttagc ggatggaatt ggtcattact tttggaattc ggcaatcatc
    12241 acaatcattg cagtagttgc tgtgacagca attattccta tggctgctta ttcactagca
    12301 agaaaaatga ctcgtgggaa agcatacgga ttgatgtatt tatttttaat tttaggaatt
    12361 tttgttcctt tccaagtaat tatgattccg attaccgtta tgatgagtaa aattggttta
    12421 gcaaatatgt ggggattaat cttactctat ctcacttatg ccgtaccaca gacactattt
    12481 ttatatgttg gttttattaa actaaatgtg cctatcagtc tggatgaggc tgctgaaatt
    12541 gatggggcta gtcactttac aacttatttc aaaattgttt tcccaatgtt aaaaccaatg
    12601 catgcgacga ctattattct taatgctcta tggttttgga atgatttcat gctaccatta
    12661 ttaattttaa atcgtgattc taatatgtgg acactaccat tgtttcaata taattatacg
    12721 ggacaatatt ttaacgacta tggtccaagt tttgcttcct atacagtagg aattgtcacg
    12781 attacactag tttatcttgc tttccaaaga agtattatag ctggaatgag caatggagca
    12841 gtgaaataac caataaatta ttctcattat aaaggagttc ataaacttat gattatattt
    12901 gacaatcaca cacaaacatt tcatttacaa aataaaaaaa ttagctatct attaggaatc
    12961 gaagaaaata attatttaac gcatctttat tttggaaaaa agattacgca ctattctggt
    13021 gggtatcgct acccccgaac agatcgttcc ttctcaccca acaatgcaaa tgcgcaagat
    13081 cgtttattat ctttagacac attaccatta gaatttccag gatatggaca tggtgatttt
    13141 agagaaccgg cgcataatat taaattagga aacggtagtc gtgtaaatga ttttcgttat
    13201 gatagctacg aaattcaaaa aggaaagccc agcttagcgg gattgccagc cttatatacg
    13261 gaagatgatg atgaagctga aacgctgatt attcaattga aagacgcgat tagtggattg
    13321 actttacagt tgttctacac aatttatcaa aatcacagtg taattattcg ttctacgcaa
    13381 ttagtaaata atagtgatga aacagtggag attaaccgct tagctagtca atcaattgat
    13441 tttccaaatc gtccactcga gttaattcat ttaaatggta cctgggcaag agaacgtcaa
    13501 atgactagag aaaaaattca tacagggata aaagtattag atagtaaacg cggatcaagc
    13561 agccatcaac aaaatccgtt tgttgcttta gtagatgcca aaacagatga atttcaagga
    13621 gaggcatatg gatttaattt agtttatagc ggtaatcacg aaacggtgat tcaaaaagat
    13681 ccatttgacc aaacacgggt cattatggga attaattcat ttaattttgg ttggcaacta
    13741 aaaactggcg actcatttca aacgccagaa gtcgtgatgg tttatagtaa tgaaggtctg
    13801 aatacgttat cacagacgta tcatgaattg tataatcacc gtttaattcg tggaaactat
    13861 cgtttaaaag aacgtcccac gttaatcaac aactgggaag caacgtattt tgattttaac
    13921 gaagaaaaaa tcatgaatat tgtcgatgat gcgaagaact tgggcattga aatgtttgtt
    13981 ttagatgatg gttggtttgg cggtcgtgaa aatgacttca cttcattagg tgattgggtt
    14041 gaaacagaag gcaaacttgc ttgtggtttg gaaaatttag ccaagcaagt gcatgataac
    14101 gggatgaaat ttgggatttg gtttgaaccg gaaatgattt ctgaagatag tgaactattt
    14161 cgagcacatc cagattgggc attgggtatt ccaggtcgtg accgctcaat tagtcgaaat
    14221 caatatgttc ttgatttttc aagacaagat gttcgtgata atatctatca acaaatgacg
    14281 gcgatttttg atcgtgtacc gattgattat gtgaagtggg atatgaatcg aaatatgaca
    14341 gaagtctatt ctgtcttgtt ggactcaacg atgcaaggtg aagtggctca tcgttatatg
    14401 ttagggttat atgaattttt agaaaagtta acaacgaatt atccaactat tttatttgaa
    14461 agttgttcgg gtggaggcgg tcgttttgat gcaggtttct tgtattacat gccacaaact
    14521 tggacgagcg acaataccga tgccgtggct cgtctgaaaa ttcaatatgg aaccagctta
    14581 gtgtatccaa tttcaagtat gggtgcgcat gtttcagcag tcccaaatca tcaaacagga
    14641 agagaaacga gtttaactat tcgtggcaat gctgcgatgg ctggtgtttt tggttatgag
    14701 ctagatctaa cgcaattaac ttctgaagaa aaagcagaaa ttaaagaaca agttgccttt
    14761 tataaaaagc atcgccaact tttgcaatat ggaacttttt atcgcttgga aagtccgttt
    14821 gaaggcaatg atactgcatg gatgtttgtc tcaaaagacc aaacagaagc catggtcttt
    14881 tatttccgag tattagcaga agctgcgcat ccattagtca cattgaaatt agcaggatta
    14941 gcagaagaag caacttatca aatggaagat gtaacatttg ctggcgatga attgatgaat
    15001 ctcggttttt atattgactc agaactacat ggtgattatg cgacacaacg cttttatttg
    15061 aaacagatat agtagcttga atttacaata taagtgcaac accaaaagag actcattcca
    15121 aacgaagaaa atatgtttta tttacacgca tttactaaaa agcagcctga tttgaattgg
    15181 gaaaatgaag aagtacgtga agaaatctat gcaatgatta actattggtt agataaagga
    15241 ctaggcggat ttagaatcga tgcgatatta aacatcaaaa aaaaactaga gtatggcatc
    15301 tttgaatctg atggagaaga tgggttagcc ttcattgggc cctggattct taatcagtcg
    15361 ggcattgaag tgtggttaaa agaaatgaga gagcggacat ttaaaccaca taatagtatg
    15421 acggtagcag aagccgatgt tcctaatgaa cgtttagatg aatatattgg tgaagatggc
    15481 tattattcga tggtttttga ttttagttat acggatattg atgtacctga aactggtgaa
    15541 tggtttaaag atagccaatg gacatggacg gatatgcgta ccaatatttt tacgaatcaa
    15601 ttagttacgc aagataaagg ttggggcgcc ttatatttag aaaaccacga tcaaccacgt
    15661 tcaattaata aatatattcc agaagaatgg atcaatgatt atagtaaaaa aatgttggcg
    15721 acgcttttta tgttactaag aggtacccct tttatttatc aaggacaaga actaggaatg
    15781 accaacatag aaatggcatc attagaagat tttgatgatg tagctacgca ttctcagtat
    15841 aaacgggcat tagaatatgg attggcacca gagcaagcat tgaaagctgt caataaacga
    15901 agtcgggata attctcgcac acctatgcaa tggaatacgc aaaaaaatgc cagattctca
    15961 acttctgata atgtatggtt aaaagtaaat cctaattatc cagaactcaa tgcagaagct
    16021 cagttaacca atcaacagtc ggtattcaat tattatcatc aattaattaa attaagaaaa
    16081 aatagtgtat acaaagacgt gctaatctac ggacaatttc taccagtgaa agataaaaat
    16141 gaacatattt tgctttatga acgccaatta gaagataaaa tcgtattagt tatcttaaac
    16201 gttacagcaa cagaacaaga atattatgta gatgaacagt accaacaagt attaatcaat
    16261 aattatgaag atgttttatt agatcaaagt cgaaaattgc gtttacgacc gtttgaaagt
    16321 attgtattag ctaattatgg aggacaatta tgaaaattaa aaacgaagca atgctcatca
    16381 cctatgcaga tagtttagga aaaaacatgg gcgaattaaa acaagtctta gacaaacatt
    16441 tacaaggtgt tattggtggt gtacatctgt tgcctttttt tccatcgaca ggtgatcgag
    16501 gctttgcgcc gagtgattat acgacagttg atccagctct tggaacatgg gaagatgtgg
    16561 aagcattagg tgaagattat tatttgatgt ttgattttat gattaatcac atttctcgtg
    16621 aatcaaaatt tttccaagat ttcaaaaaaa accatgagca gtcaccatac aaagaaatgt
    16681 ttatccgtat tcatgaattt ttcccagaaa atcgaccaac acaagaagat attgatttaa
    16741 tctacaagcg taaagacaaa gcgccgtttc aaacggtaga atttgcggat ggtacgacag
    16801 aagaggtttg gaataccttt ggggaagaac aaattgattt agatgtgacc aaagaagttg
    16861 tgaaagaatt tattcgagaa accatcaaag atatggcgaa ccatggctgt tctttaattc
    16921 gattagatgc gtttgcttat gcgattaaaa aattagatac caatgatttc ttcgtggaac
    16981 cagatatttg ggaactttta gacgaagtgc gtcaagaagc agcgaaatat gacgtcgaat
    17041 tattaccaga aatccatgag cattattcca ttcaaatgaa aattgccaat catgattatt
    17101 atatctatga cttcgccttg ccaatggtca ctctgtattc actttatagt ggaaaatcag
    17161 aacgtttagc caattggttg aaaatgagtc cgatgaagca gtttacaaca ttggatacgc
    17221 atgatggaat tggtgttgtg gacgctcgtg atttattaac agatgaagaa ttggattaca
    17281 cctctgagga attgtataaa gtcggggcga atgtgaagaa ggtctattca agtgccaatt
    17341 acaataattt agatatttat caaattaaca gcacctatta tagtgcatta ggcgataatg
    17401 accgtagcta tctgttagca cgtgcgattc aagtatttgc gccagggatt ccacaaattt
    17461 attacgttgg gttattagca ggaaaaaatg atattgaact gttggaagca acgaaagaag
    17521 ggcgtaatat taatcgtcat tactatgatt tagccgaaat tgaagaagaa gtgcaacggc
    17581 cagtgattca acaattattt gcacttttaa aatttagaaa tgaatctgcg gcgtttgatt
    17641 tggatggaac gattgaagta gcaacgccat cagaatcaga aatagtgatt acacgtaaaa
    17701 ataaaaacgg tagtgatagt gcaacattaa cggctaactt ggatacgaaa gaatttatca
    17761 ttgagtctaa agatataaaa attttcacta gtgttgcatt aaaattttaa cctattccat
    17821 agaacaaaaa atcctttata ctagcacttg gatgacttat ccaagaccaa taaaaaggac
    17881 tcatacatgg ataagtataa aacaaataca gcatttaata aatggttttc tgccataaaa
    17941 ttaggaaaac tttctgtcaa ctcccaaaaa tgtatttttg attacaattc gtatcgtaaa
    18001 aaactgtctt ttgaagctgt gctcaagctc tttctctacg cagttaatga agaaaaagaa
    18061 agtttgcgtg atttgagtac agctcttatc gaccgatcgc tccaactaga aaccgatgtg
    18121 tcatcgatta gtcataccca attgtctcga gcatttaatg cacttgaacc taaagtgtta
    18181 gaagaaattt ttcaacaact gttagaaaaa gttcaacatc aaatcaaacc aaccaagcga
    18241 aatcatctgt atctgattga ttccagtacg ttttcgctta gtttaaaacg tcataagtgg
    18301 gcgactttcc gtcaaacaaa atcaggtgtc aagttacact taaactattg ttatatggac
    18361 gatactacga tgtatccaac tgattttacc ttaacaaatg cccatgaaca tgatctcaat
    18421 caactaccag tattggtgaa tcaaccagag gcaacttacg tgtttgatcg tgggtattag
    18481 gactttgaaa agatggatca gatgcattgg gatggctatt tcttcgtcac tcggattaaa
    18541 aaaaacacca aagttcacgt gatagatact ctggaaacat cgccagaaac agaaattcta
    18601 cgagacgagc tggttcgttt aggatctaaa acctatctaa ccgccaattt ccgactagtc
    18661 actgttcaag ataagaacgg acgtgtgttt caatttatca ccaatcgaat ggacgtctct
    18721 tcaaaagaaa tctctgatat gtatcacgct cgttggcaaa tcgaattgtt tttcaagcac
    18781 atcaaacaac acatgaccat taaaaccttc ttttctcaaa gtgaaaaagg cgtgcagaat
    18841 caattgattt taacgatgat ttccgctctc ctcacgtttc tcatcaaatt ggagacgaaa
    18901 acggaaaaat cagtgttcca aatcaaacga ttctttcgtt atttactgtt tcagcccttt
    18961 gaatgttgtc tagaaaaatt aattcccaca tgagaaaaaa gaaatttggc tcgttacttt
    19021 tggttttcag ttgaataaca gataaaaaat tttctggaaa aagtcatcct ctgcgtcagc
    19081 acagtttttt ttgacttatt ttcagaggaa atagttttca gaatattcta aaatgtttta
    19141 tgcaacacta gtgatatttt ttagaattag atttctaatg tgcatttttt gagtattaag
    19201 gagcaagtat acaaagattt ataaagctga ttaaatttga ataaaaatat gcaggaacgc
    19261 tactattttt agtagtgttc ttttttatat ttgaataaaa aatgtggtgg ttaaatggtg
    19321 gttataacaa aaaatgaatt aatgaaatta ggttatggga agtatcagtc tgagagaatc
    19381 atacgacagg caaaagcaat tatggtttca aaaagctata cctattacga aaatagaaga
    19441 ttaggactag tacctgttga atctgtagaa gaagttttag gtatgaagat agaggtagag
    19501 gaggattttc agaatcatgg ttaattttaa aaaagataaa aatgggaaat atcattttac
    19561 tgctagtttg ggattcgaca aacgaacagg gaaaagagta caaaaaaacg tagaggggtt
    19621 aaaacagtta aagaagaaaa aaagcgtata ccgaacttgt ttctaattat ggaaaagaag
    19681 cagaaacttc tcaaagtact atgttgtttg aggagttttt ttataaatta ttcctacctt
    19741 attatcaagg gaaagtagaa gaaagaacat ataaaaatcg gttatctaca atcaaaaaaa
    19801 cttttcttat ttctttaaaa tggagttaag agaaatacgt ccaattgaca ttcaaaagtg
    19861 gcaaaataat ttattgagca aatataaaaa ttcttatgta agaaacatgt atgggatttt
    19921 tcagatgtca ttagatcgag cagttgtttt gggactactt gaaaaaaatc ctgcaaaagt
    19981 tattggaaat gtgaagaaaa ggaaaactac agttgatttt tggacaaaag aagaatttac
    20041 aaaagttatt tcaacttttt atattgatga tttgtaccaa cattttggtt ttatttgtat
    20101 gtggttactt tttatgaccg ttatgcgttt gggagaagcc actgcattaa cttggaaaga
    20161 tattgatttt gaaaataaaa acttgcgtgt gaataaaaca ctctattata agagagccaa
    20221 aacctatgag tttgtagaac ccgaaacggc ttctagtatt cgtacaatcg aactagacga
    20281 caatacaatt acctttttaa aagcttggaa ggagcgtcaa gtttcaatag gaggcatgtc
    20341 gtttgtactt tcttataatg gaacacctac tcagaaatat acaatccgtc atattatgag
    20401 aagacatgca acgatcgttg gtgttcatat aatccgcata cacggattaa ggcatagtca
    20461 tgcttctttg cttatatcac aataaaaaga tgcacttcga attaaagaaa ggttaaaaca
    20521 tgctgatgta caaacgactt taggaactta tggtcatttg tatctaaatt cattgagaga
    20581 gataacaaat gatcccacag attatatcca aactaatttt tctaaaatca aaccgacacc
    20641 tatttacgat aataaaaaat aaatgataaa caaaagtagg atcacaccaa aagaaacaca
    20701 ctagaaaaac tataatacag gcttttctag cgtgttgttt cttatttccg cctatttata
    20761 cattatactc ataaagcatt taatgcatcg atcgtagccg cattgtctat atgggtatat
    20821 aaagttgttg tattggtact tgaatgtccc aattgttggg cagttaatac caatgaattt
    20881 gtctgttggt agagtctagt agctaatgta tgacgcagtt tgtgagggct gacttgaatt
    20941 ctaaagcctt ttgaatattt tgctaccatc cgttcgattg caataccgga tatacgcttg
    21001 gcttctcctt ggtaaattgc tagaaataaa gctttttcgt tttctgttac tttatatcgt
    21061 tctttccgaa tagctagata attagctaaa tattccattg cgaatgctgc tatattgaca
    21121 gcatctcttt tatttccttt tctagttact accacattat tcttatctaa agataggtca
    21181 tctaaattaa tatttataac ctcggagagt ctcaatccag atcctaaaat cagtgcattg
    21241 atcgccaggt ctctttctac atctcgttga taatagatca aagctcgttt actcaaagta
    21301 tttttatatt cattggcaat ataatctaga taattttgtg tatccttatc tagaaataat
    21361 tttttctcaa tggcattggc acgtgcagct aacgtagttt tatctgtgat ggttgctact
    21421 tgatccatag gattattgtc taagtatggt tttcctagag gattattgga acttttcgaa
    21481 agatatttaa atagaacctt taatgccgtg atatttctat tgatagtaga agtagataga
    21541 cctaagttat tattatcttt gagcttagtt tttgttgtct ctttttttac attttctttt
    21601 gtgcgacgtt tcaaatgaga tttgtacaag tttaggtcat aggcagttaa ctgtcttatc
    21661 gaggtgatag aagtatcagg aacgatagac tcactcagaa gccattcata aaagagctta
    21721 taatttttta gatattctaa agtggttcga ggtgattgat ctttatcctc acgatcttga
    21781 acaaaatctt ttatataatc gggcatatga agtatgattg tatctatttt ttgatatatt
    21841 tctgtatttt tcatgtcatt catcctttca ataagtaggg gagaagagta tctctataat
    21901 tggactatac caattataga atgttttgtc aattggtatg ctctaaaatt tattaatttg
    21961 tttcaaaaaa agcatataaa caggtatacg tttaataatt taaaataaat ggtatacata
    22021 aatagggaag gaaatagaag ggagcaatct aaattttttg aattttctta attaattata
    22081 tagaaagaac aatctttcta tagagttaga catgagaggg actatgcttg atttaaaatc
    22141 aacaagcgta tatactgtat atacaaagaa aaaggaggga gaagactatg gcaattaaag
    22201 aaagaaaatt tagggaagtg gggaactcag tagtattaac tatttcaaag gagtttctta
    22261 aagaaaatgg gttaaatccc ggagatacgg tttttttaga tgaagaaaaa cttcgagcgg
    22321 caattacaaa aaaacaagcc ttatctgatg aaattgattt agcaatatct caagcattaa
    22381 ctgaatatga tactactttt agagagcttg tcgataaatg agtattcatt acctagaagc
    22441 caaagatatt ataaaaatga atgtaatcca aataaaaaaa tactctccta atgaacccct
    22501 tggtgtaaag gatgcgaatg ctttagaaat gtgtgtaggg caacttcgag cctcagcttt
    22561 tggtgaagag gtttatcctt cgatttatga aaaagcagct atcctactta tacaattgat
    22621 aaagaaacat ccttttcaca atgcaaataa acgtactgct ttcttagcta cttttgtctt
    22681 tttgaaaatc aatggacagc ttttagctat tgatcaaaaa gatgctgtaa atttagcggt
    22741 atatattgct acgtatgata aggatttcga tcaactaaaa tatgaagtga tcaacactat
    22801 aaagacacat acgcagccca tttaaggagt tactgtatgt gtctttatag ggttattagc
    22861 tgctatagcg aaataattga agtaaattac aaatagctat aattcaaatc caactatata
    22921 gaaaacgtaa attttctata tagttggatt aagataacga ctatttattt tatcatattt
    22981 ttactatacc ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata
    23041 ctactagttt gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata
    23101 cacctacaac ttataaattg atggtattta gataacacga aagaataaaa ataacagctg
    23161 atagtaaagt ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg
    23221 cttctgaaaa cttatgaaat gaaagacctt ttacatcttg cagaaatcgt tactgggatt
    23281 aaactaagca ataaaaacta atgttcccct cattctttga acttctacta tttgatttat
    23341 ttattctgat aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt
    23401 aagatctctt cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag
    23461 aataaactgc tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc
    23521 caaattataa accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa
    23581 gtcagaagat ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata
    23641 tttaaaggga attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc
    23701 tttcccctgc tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta
    23761 tcgaagtgag tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac
    23821 cctctacggc attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca
    23881 aagaacttat gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat
    23941 tgcttagcag taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct
    24001 aaaagtccca aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc
    24061 cgttttggtt gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact
    24121 cattgaatca atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc
    24181 tttcaatggt agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt
    24241 ccatttaaat tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca
    24301 ttttagaatt aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc
    24361 cacttcctca actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata
    24421 tttagtataa gtcctattta tttctttttt taattcttct ttatttttat atgttctcat
    24481 tatattacct ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga
    24541 tattttaatc cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga
    24601 atgatttata aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct
    24661 aaaaaatacg aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt
    24721 cgtaaatatc aaaatcatgt taactactat caattactaa taaaaactac cactttaagc
    24781 gttaagaact gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc
    24841 acgaaagtgg taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat
    24901 tgcgcgttgg gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct
    24961 catcagatga aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa
    25021 aagcgattgc catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa
    25081 gatcatcctt tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca
    25141 atgatgtttt caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa
    25201 aagaaaattc accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt
    25261 ttttccaaaa ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata
    25321 acttcttttt aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg
    25381 atttttatga ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag
    25441 tatggacgtt tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt
    25501 agtgaactac ccccactta
//
LOCUS       DAYYBG010000029        25363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000029 DAYYBG010000000
VERSION     DAYYBG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.029"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACHAUN_001225"
     CDS             complement(164..388)
                     /locus_tag="ACHAUN_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8459936.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACHAUN_001226"
     CDS             complement(586..2166)
                     /locus_tag="ACHAUN_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8459937.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACHAUN_001227"
     CDS             complement(2567..3943)
                     /locus_tag="ACHAUN_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8459938.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACHAUN_001228"
     CDS             complement(4071..5159)
                     /locus_tag="ACHAUN_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8459939.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACHAUN_001229"
     CDS             5331..5765
                     /locus_tag="ACHAUN_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8459940.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACHAUN_001230"
     CDS             complement(5843..6376)
                     /locus_tag="ACHAUN_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8459941.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACHAUN_001231"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACHAUN_001231"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8459942.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACHAUN_001232"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACHAUN_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8459943.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACHAUN_001233"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACHAUN_001233"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8459944.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACHAUN_001234"
     CDS             complement(10101..10679)
                     /locus_tag="ACHAUN_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8459945.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACHAUN_001235"
     CDS             11001..11471
                     /locus_tag="ACHAUN_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8459946.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACHAUN_001236"
     CDS             complement(11579..12805)
                     /locus_tag="ACHAUN_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8459947.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACHAUN_001237"
     CDS             complement(13559..13759)
                     /locus_tag="ACHAUN_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8459948.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACHAUN_001238"
     CDS             complement(13893..14501)
                     /locus_tag="ACHAUN_001238"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8459949.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACHAUN_001239"
     CDS             complement(14586..15056)
                     /locus_tag="ACHAUN_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8459950.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACHAUN_001240"
     CDS             complement(15104..16018)
                     /locus_tag="ACHAUN_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8459951.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACHAUN_001241"
     CDS             16164..16967
                     /locus_tag="ACHAUN_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8459952.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACHAUN_001242"
     CDS             17145..18092
                     /locus_tag="ACHAUN_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8459953.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACHAUN_001243"
     CDS             complement(18160..18453)
                     /locus_tag="ACHAUN_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8459954.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACHAUN_001244"
     CDS             complement(18481..19146)
                     /locus_tag="ACHAUN_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8459955.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACHAUN_001245"
     CDS             complement(19286..19918)
                     /locus_tag="ACHAUN_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8459956.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACHAUN_001246"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACHAUN_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8459957.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACHAUN_001247"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACHAUN_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8459958.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACHAUN_001248"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACHAUN_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8459959.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACHAUN_001249"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACHAUN_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8459960.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="ACHAUN_001250"
     CDS             23883..25052
                     /locus_tag="ACHAUN_001250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8459961.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         7626 a         5108 c         4077 g         8552 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac attatttgac
    25261 aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg ctagcaaatc
    25321 attcatggct tgttcttatt tttaatttta tgcaataaaa aag
//
LOCUS       DAYYBG010000030        25358 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000030 DAYYBG010000000
VERSION     DAYYBG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.030"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHAUN_001251"
     CDS             complement(132..509)
                     /locus_tag="ACHAUN_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459962.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHAUN_001252"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHAUN_001252"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8459963.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHAUN_001253"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHAUN_001253"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8459964.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHAUN_001254"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHAUN_001254"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8459965.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHAUN_001255"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHAUN_001255"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8459966.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHAUN_001256"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHAUN_001256"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8459967.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHAUN_001257"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHAUN_001257"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8459968.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHAUN_001258"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHAUN_001258"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8459969.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHAUN_001259"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHAUN_001259"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8459970.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHAUN_001260"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHAUN_001260"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8459971.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHAUN_001261"
     CDS             10661..11575
                     /locus_tag="ACHAUN_001261"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8459972.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHAUN_001262"
     CDS             complement(11708..13273)
                     /locus_tag="ACHAUN_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8459973.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHAUN_001263"
     CDS             complement(13266..14717)
                     /locus_tag="ACHAUN_001263"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8459974.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHAUN_001264"
     CDS             complement(14731..15747)
                     /locus_tag="ACHAUN_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8459975.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHAUN_001265"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHAUN_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8459976.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHAUN_001266"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHAUN_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8459977.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHAUN_001267"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHAUN_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8459978.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHAUN_001268"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHAUN_001268"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8459979.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACHAUN_001269"
     CDS             21340..22764
                     /locus_tag="ACHAUN_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8459980.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACHAUN_001270"
     CDS             complement(22806..23063)
                     /locus_tag="ACHAUN_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8459981.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHAUN_001271"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHAUN_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8459982.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACHAUN_001272"
     CDS             24337..25314
                     /locus_tag="ACHAUN_001272"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8459983.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8201 a         4317 c         5696 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcgccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAYYBG010000031        24392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000031 DAYYBG010000000
VERSION     DAYYBG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.031"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(187..2532)
                     /locus_tag="ACHAUN_001273"
     CDS             complement(187..2532)
                     /locus_tag="ACHAUN_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8459984.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2811..3713
                     /locus_tag="ACHAUN_001274"
     CDS             2811..3713
                     /locus_tag="ACHAUN_001274"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8459985.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3924..5699
                     /locus_tag="ACHAUN_001275"
     CDS             3924..5699
                     /locus_tag="ACHAUN_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8459986.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5765..7351)
                     /locus_tag="ACHAUN_001276"
     CDS             complement(5765..7351)
                     /locus_tag="ACHAUN_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8459987.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7602..7826
                     /locus_tag="ACHAUN_001277"
     CDS             7602..7826
                     /locus_tag="ACHAUN_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8459988.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7968..9719
                     /locus_tag="ACHAUN_001278"
     CDS             7968..9719
                     /locus_tag="ACHAUN_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8459989.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9719..11503
                     /locus_tag="ACHAUN_001279"
     CDS             9719..11503
                     /locus_tag="ACHAUN_001279"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8459990.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11838..12690
                     /gene="licT"
                     /locus_tag="ACHAUN_001280"
                     /pseudo
     CDS             11838..12690
                     /gene="licT"
                     /locus_tag="ACHAUN_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12787..14673
                     /locus_tag="ACHAUN_001281"
     CDS             12787..14673
                     /locus_tag="ACHAUN_001281"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8459991.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14714..15616)
                     /locus_tag="ACHAUN_001282"
     CDS             complement(14714..15616)
                     /locus_tag="ACHAUN_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8459992.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15846..17249
                     /locus_tag="ACHAUN_001283"
     CDS             15846..17249
                     /locus_tag="ACHAUN_001283"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8459993.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17249..18682
                     /locus_tag="ACHAUN_001284"
     CDS             17249..18682
                     /locus_tag="ACHAUN_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8459994.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18827..19753
                     /gene="trxB"
                     /locus_tag="ACHAUN_001285"
     CDS             18827..19753
                     /gene="trxB"
                     /locus_tag="ACHAUN_001285"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8459995.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19966..21693
                     /locus_tag="ACHAUN_001286"
     CDS             19966..21693
                     /locus_tag="ACHAUN_001286"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8459996.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21709..22059
                     /locus_tag="ACHAUN_001287"
     CDS             21709..22059
                     /locus_tag="ACHAUN_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8459997.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22153..23013)
                     /locus_tag="ACHAUN_001288"
     CDS             complement(22153..23013)
                     /locus_tag="ACHAUN_001288"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8459998.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23105..23533
                     /locus_tag="ACHAUN_001289"
     CDS             23105..23533
                     /locus_tag="ACHAUN_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8459999.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23695..23871
                     /gene="rpsU"
                     /locus_tag="ACHAUN_001290"
     CDS             23695..23871
                     /gene="rpsU"
                     /locus_tag="ACHAUN_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8460000.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23898..24344
                     /locus_tag="ACHAUN_001291"
     CDS             23898..24344
                     /locus_tag="ACHAUN_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8460001.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7721 a         4362 c         5191 g         7118 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctctggtttt
       61 tcacattttt acataaattt gtcttaatat cgtagatgta agcttttgaa caccatttat
      121 tcggaaaaag ggagctgtga caagattttt gtcgcagctc cctttaattc tgattaatct
      181 attagatcaa tcatctttat cgtctgattc tttggtctct ggattcgctc tagcataagc
      241 tgaagcggtg ttccagtaat ctgtatagtt ttcatcagta ccgcctacgc cgaatttaaa
      301 ggcgcttttc ttcggaccat tttttgccca aagtgactcc actgtttttc ctggcgtttg
      361 gatggatttt ttattgacag taacttttcc agctgtttgt cctgtgaacc cagatacttt
      421 tttcttcatc acatcggatg ataagtcaaa tttctcatca gtgccgaaaa tctcagggtt
      481 tgcttggtaa gcttgattga ttagattggc aagataatta gccgaacgta ttcttgcttg
      541 gctgtccatt ccagtattgt catcgtgtcc tgcccaacta ctgatagtga tagatggagt
      601 tgagacgatc agccatgaat ctcggtattc attggtagaa ccagtttttc cgacccaatc
      661 tgcatttcct agatttccgt ttaagcttga gatgacatct ttgaatggtg tagtgatttt
      721 ttcattgatc acactacgca tcatgtcatt catgatcgat gccgttgctt cggaataaat
      781 acggacagaa ttactttcat gtttataaat cacattacct gcattatctg tgatggaatc
      841 aatgatatag ccttcttcgt agactccacc atttgctaag gcctggaatc catttgtctg
      901 ttgaagcgtc gttacatcta ctgtccctaa aggagccgat tcaacgcccc aattatcatt
      961 tgctgggtag ttcatcttcg ccaagtagtg ctcgtaagcg tattgtttcg atccaccgga
     1021 attcaacaca tcttgatata agtgataagc tgggatattg ttggaccatt ccagtgactc
     1081 acggacggtt tggaaggtgt tcgacccttc attggtcgca ttgacgattt tctcaccagc
     1141 attttttcct tcttgccaag tcgttgcata atctgataca cgggattctg atccgacaag
     1201 tcccatatcg attgcaggac cgtaaacaag aaccggtttg atggatgaac cagcttgacg
     1261 ttctgtatca aaggcgtggt tattttgatt ctggctgtaa ttccgaccac ccacgaagcc
     1321 atagatacgt ccggttctgt tatccatcaa tacatttcca gtctccactt ggctaccagc
     1381 accatcatct aacatgtagc cataattttc tacgccggtt tgtaatgcag catagatatc
     1441 tttatcgatc gtcgaatgga tcgtatatcc tttgttttgg atcgtttgct gtgctttttg
     1501 ataataagct tcactgaccg atgaatctga catatcagca ttatcttttt ctgccaattg
     1561 ttgtgcaata atccttgttg cttcgttcat cacggtataa tacaagaatt ctctgtcagt
     1621 ctgttccgcg atttgttgtg ggagaaagtc ttttgtaaga tcataggctt tcgcttcttc
     1681 gtattgttct ttcgtaattt gtccttcacg gtacatactg aataaaacaa aatcttttct
     1741 ggcaagaccg gcagaaagat cttcttttag cgcgcctgta ttcgtgtatg gactatacgt
     1801 gatagggctt tgaggcaaac cagcgatata agcagcttgt ggcagagtca catcctttgc
     1861 atctacacca aagattcctc tagcagcttc ctggactcca gcaatatttt gccccttatt
     1921 attccgacca aatggtgaga cattcaaata cgtagtaatg atctcatttt ttgaaaagtg
     1981 tttttctact tgtgcagaaa gaacgatctc gtttgcttta cgtttgaatg ttgtttcatc
     2041 ggttaggatc tgttgtttga ctaattgctg tgtcaaagtg gatcctccag acgagccgat
     2101 gccagtcgct tctgaaacta gtgctcgtaa aacagcttta gggacataac cttgatgttt
     2161 atcgaagtat tcatcttctg tactgataat cgcggttttc aataaaggcg aaatcttatc
     2221 agatgaaata gtcgtccgca tcagatctgt ctggattttt gatatctctg tattatctgc
     2281 ataaacaatc tttgacgttt ccgtaatgtt tcctagttct gtctgcagtt cttttttagt
     2341 cggcaattgt gtgtcttcga caagataggc aaagtagcca gcacctattc cgatacctag
     2401 agctgcgaac aacaaaagaa gaacagtaat aaaaacagtc aatgattgaa acactctaaa
     2461 aataatctta ggaacaaacc atttttcttt ttttcttcta ttctgttttt ttcgagtatt
     2521 tttgcgtggc aaaagggtca cctcaactaa tggttttatt tcgctaatta tagcaaataa
     2581 taggacaaaa aaacagacaa ggctgttttt gacaggaatt tctttttcga atgagataaa
     2641 acgtgaggaa atgatctttt ttatgaaaga gttcttaagc ttttctttag ggaaagtttc
     2701 agttactatg gagaacaggg caaagaaaaa caaaccattt tctttgtaca tcatcttatc
     2761 aatatacagg aattgttcga aacaattgcc tttcaaggag gaatccatcc atgttattag
     2821 gctcacatgt cagcatgagc ggaaagaaaa tgctgttagg cgcggcagaa gaagcagcca
     2881 gttacggatc ttcgaccttt atgatctata caggcgctcc tcaaaatact agaagaaaag
     2941 ctatcgaaga gatgaatatt gatgccggaa aagcatttat ggcagaaaac ggactaacaa
     3001 atatcgtcgt tcatgcgcct tatatcatta atttgggaaa tacgatcaaa ccagaaatgt
     3061 ttccatttgc tgtacaattt ctgagagaag agattgtccg tgcggaagct ttaggtgcta
     3121 ctcaaatcac ccttcaccca ggcgctcatg taggagcagg ggcagaagct gggattgcta
     3181 gaattattga ggggttgaat gaagttttga caaaagaaca gacggcacag atcgctttgg
     3241 agacgatggc agggaaaggt acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca
     3301 tcgatggagt aaaattgaat gaaaaattat ccgttacttt tgatacatgt catacgaatg
     3361 atgccggata taatataaaa gaagattttt caggtgtaat ggaagagttt gataaggtca
     3421 tcggattaga tcggcttaaa gtactgcata tcaatgattc taaaaatcca caaggttctc
     3481 ataaagatcg gcatgccaat atcggttttg gaacaattgg atttgacaca ttgaacaaaa
     3541 tcgtccatga tccaatgttt gacaacattt cgaaaatatt agaaacccca tatgtcggta
     3601 aagataaaaa gaaagcttac gcaccatatg gaaaagaaat ttccatgttc aaagcgcaag
     3661 cattcgatcc cgaagtgttc cctgagttat tagcagaaga agtcacactt taagttttct
     3721 acctgacagc aacaaaaaag gattgggcag tctgccttgt ccttttttgt ttaagaaaca
     3781 aaggagtgct ttttgcattc gttttcgcca tgctcctttt gccccctctt tttaaattca
     3841 gacaaacagg gtatactgat agaaaagtaa aaaacgaaaa gtttcacaaa caagtagcaa
     3901 aacgtgattg aaaggacgaa aagatgctga acattttaaa ccccttgatc gaccaactaa
     3961 aaaagaaaca tacatacttt attcttggca tagccgtatt atttacaatt attcggttgt
     4021 atttatatag taaagcaatc tatggaacag atatgtcggc gacctatgat gatcagctgc
     4081 tgattcacta tgctcaaaat attttggatg gtaaatggct aggggagtat acgactacaa
     4141 cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa ctgcatcttt
     4201 cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt gcactcgcgt
     4261 ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat ctatttttat tttttgtttt
     4321 ctccgattag ttttacacat gaatattcca ctcgaacata tcgtaatgcc ctcgtgattc
     4381 cggctgttga gatcattatc gcttgtttat tagctattta ttaccgcaaa tacggttcta
     4441 gcagaaaatt gctgccatgg tttttagggc tttctgtcgc ttttcctttc ttttggttca
     4501 ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt agctttgggt atcagtttcg
     4561 tccagatctt gctagggcaa gacttttctt tcaaagaagt aaagaagtta aaaaaacgac
     4621 tctttcttct ctcgaaacgg gaatggctga aactttcatt gtttttactt cctatagttg
     4681 gcttttccat cgtctatcag tggatcgaat ataaaaatga gacaacatac ggtattgcaa
     4741 ccatcaatga ccgttccagt ggagaatttg gacgtttgat gaaacaactg atccgaatag
     4801 atgatgaaac gaacttgaat aaagaaaaca gtgagatatg ggtctcacat gctgcactaa
     4861 ataaagcatt agccgcatct cctacattcc atactgtttc tacccgtatt gacgaattat
     4921 atacgactca cgcgtggact caaggtggaa aagtaaaaga attacccgga gatatcatct
     4981 tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat gcaaaagaaa
     5041 ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga agcctatcac aagggaacca
     5101 tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga tggaaagacg atgagtgatc
     5161 tgccaatcgt attcgatttc cttcaatcag gatacgctta tgcatggcga tataaagatt
     5221 tccatcaagg aggaaattat agtttcggaa cgcaagaaca agtcaaacag gctcaagaat
     5281 tattaaacct tccgctaatg gagaattgga tcgatagtaa tgaaccgaga acacctaatt
     5341 tccaattaac aaaatcagca aaagtcgcga atgtcatcat aaaaatttac caagttctgg
     5401 ctattcccgt gctgattgct tcaatagcag ggatattact tatgatacta ggaaccgtat
     5461 ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtggtcacc ggattaatac
     5521 tgacacaatt ggtttttctt ttcggtgtct catggttttg ttcttatgcg ccagaaaaaa
     5581 aggactattt tttaagtgtc tacactggtg caggcgtgcc attaacgcaa ttcatcgtag
     5641 taacaagcat gctagttttc agtaaattaa atctgaaaaa agctttatct tctaaataaa
     5701 aagacccttg gtagctagat ttttaaccta actatcaagg gtctttgtgt ggaaaaatca
     5761 gtttttattc gatttcttct accgtttcct gcaaaagctg ttcatatttt ttgtttcctt
     5821 tatataaatc accaatcgtc catgccaaca ggagaaggac aagaacaatg attacataag
     5881 cgatgtgatc agccaatgct agttgagcgg aagtggcttg ctcgccgaag aaactttcga
     5941 tctgtcctgg taatcctcgt aagttcaagt aagttagact aatcacagag aaccagccaa
     6001 gaagttttac gatgaatgaa tttttaaatc ggctacccat ctccgcttgg ctgttggtca
     6061 tcatcagtaa aggaatcatc gaaaatggca acgcaaaagc caagaacacc tgggattcat
     6121 tcattaagtt attcaaagcg gcatgttctt cgatcgttcc ttttgtactt gtaaacaaaa
     6181 cacaaatcaa tacaggaatc acagaaagca atcgtgtgac cagtcttctt agccaaatcg
     6241 ggatacgcat atggatgaat ccttccatga tgacttgacc ggtcaacgta ccagtaatag
     6301 tagagttctg gccagaagca agaagtgcta cagcaaataa agtggacaag ataccagatt
     6361 ttgctacagc aatcaaaata ccattgctca tagctgatgg atcagataaa gcttcataca
     6421 gaccaaaaaa ggaaggatct tggacagaac cggttttgaa gacagcgaca cccatgatca
     6481 atagcagtgc attgactaga aaagccattg acaactggat attagaatcc caagtagaaa
     6541 aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc gatcttccga gtttgtgaaa
     6601 ctgctgaatg aagatataag ttatgcggca tgactgttgc accaataatc cctaaagctc
     6661 cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga gaatgtatga ctgttaggga
     6721 taaagccagc aaataaactg tgccaatctg ggttagacaa gccaacttga tagacaaaaa
     6781 cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc gatcttacga aacccgactt
     6841 ttgtcagtag taagagaacg aaaacatcta atacagtaat aaagacggca atcaccaatg
     6901 gaatatcaaa aagtaagtag agggcgattg ctgcaccaat gacttctgcg atgtctgttg
     6961 ccataatggc taattcagtc aagatccata aaatgacacc caatgtctta cttgtccgtg
     7021 ctcggattgc ttgggccaaa tccatttggg tcacgattcc taattttgct gccatatact
     7081 gtaaaagcat agcaattaga ctagacatca aaataactga catcaataaa tattggaaat
     7141 tttgtccacc agtgatcgaa gtggaccagt tacctggatc catataacca actgctacta
     7201 atgcacctgg tccagagtaa gcaagaagtg ttttaaaaaa actttttccc tttggtacat
     7261 ccactgttcc atttacttca gctaaagata aaccatttgt atgttcaata agtcgatgct
     7321 tatgaaattg tttgttggat tgattattca agaaaaaacc acctttccta tttatctacg
     7381 attacattct acacctcaga aagaaaaaat taaagaaaaa gattcggtat gcctaatttt
     7441 ttttcgtggt gtgtcttgct ggttaatgaa agaaattttg ttcttctcta aaaaaaccac
     7501 tattttttgc atgaaactaa agaatacgcc tttctttttt caataaactt tagtaaaata
     7561 aatcattgag agcaattcgg aaatagaaag gagagattta tatggtatca acaggtattg
     7621 tcgtacttat tgctattatt gctttgctaa tcggcgcagt aggtggcttt ttcctagcgc
     7681 gtaaatatat gaaagactat ttagaaaaaa atcctcccgt taatgaggac atgttgcgat
     7741 caatgatgat gtcaatgggg caaaaaccat ctgagaagaa aattcgccag atgatgcagc
     7801 aaatgaagaa ccaagggaaa aaataggttc tgacccaaag actttttgcg ggctgatttt
     7861 ttcagtccgt tttttatttc gtaatttgtt agactattca gactattcag caaaaaagac
     7921 aagcacagac aatcctatgc tcttgataaa gacaaaggag gagtaaaatg tcgattttta
     7981 gaaaattagg ttggtttttt cgtcaggaaa agaaacatta tttaattggg attttctccc
     8041 tattcgcagt ggccttggta cagttagttc ctccaaaagt gattgggatc atcattgatg
     8101 agatcgccga taagaattta tcaataaaga tcatttcctg gtggatctta ttattgatcc
     8161 ttgcagcttt cttacaatat ctttttcgtt atatctggcg gacgaatatt tggggaagtg
     8221 cagctcggtt ggagagagaa cttcggcagc agctattcaa acattttaca gaaatggaca
     8281 atctctttta ccaaaaatat cggactggag atttgatggc tcatgcaacg aatgatttga
     8341 atgcaatcca gaatgtagca ggagctggaa tcttgacctt tgcagattca gtgattacgg
     8401 gcggaacaac gatcattgcg atgattttgt ttattgattg gcgcttaaca ctgattgcgc
     8461 tgattcctct gcctttgcta gccgttactt cacgagtgct ggggtcaaaa ctccacgatg
     8521 cttttcgtga ttcgcaagaa gctttttcta gtatcaatga taagacacaa gaaagtatca
     8581 ctgggatcaa agtcattaaa acatttggac aagaaaaaga agatctcgcg gattttgaag
     8641 aaaagattga agatgcaatt attaaaaaca aacgagtcaa ttttttagac tccttatttg
     8701 atccattcat taccttgatt atcggtctgt cttatgttgc aacaattatt ttaggtggaa
     8761 agtatgtctt ggaaggcacg atctcaatcg ggcagatggt ctcatttatt agctatatcg
     8821 gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt caacgtattg gaaagaggaa
     8881 atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc gtggaacaac
     8941 tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt agctatttcc tcttttaact
     9001 atccgaaaga agaatcacca gcattagaac aaatcaaatt tgcgatccaa gaaggagaaa
     9061 cattaggtat tgttgggaaa actggtgcgg gaaaaacgac aatcatcaaa ttactgatgc
     9121 gtgaatacga tgattatcaa gggaaaatcc aatttggcgg acgttcgatc aaagaatatt
     9181 cattgaatgc tttattagga tcaatcggtt atgtgcctca ggatcacttc ctgttttcga
     9241 tgaccattcg agataatatc cgtttttcta atccaaaact ttcagaagaa gaagtagagc
     9301 atgcggcttc tttagcattt atcaacaatg aaatcaagga attgccaaaa ggttatgata
     9361 ccttagttgg agaacgtgga gtctctttat ctggcggaca gaaacagagg atttcgattg
     9421 ctcgagcatt gatcaaaaat cctgaattgc tgatcttaga tgatgcactt tctgcagtag
     9481 atgccaaaac agaagaagct atcttgcata atttaaaaga aataagaaaa gagaaaacaa
     9541 ccatcattgc agctcaccgt ttaagcagcg tgatgcatgc aaaagaaatc attgttgtcg
     9601 atcaagggaa aatcattgaa cgaggaaccc accacgagct gcttgcatta ggtggctggt
     9661 atgaacgaat gtgggaaaaa cagcaattag aagcgaagat agaaggaggc gaggcctgat
     9721 ggatgaaaat tatcaatcgg aatggacaaa atcgattcca cttaaagaac agaagaagat
     9781 catcaaacga ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg ttgctatctt
     9841 atttgcgttt gccctgtctg tgatcaatgt cctattgcca aggatcatcc aaacttttat
     9901 ggatgatcat ctggctaaac agtcagctac gacacaagtt attttgtttt ttgctggtgt
     9961 gtacttgttt ggagtcatag tcaaaagtat catttggttt ttccaatggt atttgtattc
    10021 catggcttca ttgaagacct atcagtatgt tcgggtaaga ttatttgaaa agctgcatac
    10081 actaggtatg cgttattttg accagacacc ggcaggatcg accgtttctc gagtgacgaa
    10141 tgacacagaa acgctatttg aattttggta tgtctttttg atggtattga ctgggatctt
    10201 tgcagtcgtt tcttctttcg ttgccatgtt tcaaatcaat ggagaaattg ccttgtggac
    10261 tttggtgttc ttgccgattt taggaatcgt catctggtat taccagaaat tcagttctcg
    10321 tatttaccga aatatgcggg aaaaattaag tcagctgaac acgaaattga atgaatcgat
    10381 ttccggcatg cgtattatcc agcagtttcg ccaagaagct cgtttagcta aagaatttga
    10441 acaagtgaac aacgagtatt tagacacgcg ctatgcgatg atcaaaacga attcgattct
    10501 gttaagtcca atcatcaatt ttctctatgc attggcgatt gcgttggcgt tgactttgtt
    10561 tgggatcgat gcgttgcatt ctcccgtaga agttggactg atctatgcat ttacgaccta
    10621 tgtccaggaa ttttttaatc caatgtctca aatgatggac tttttgagca cctttacaga
    10681 tgggatggtt gccggtagtc gtattttgaa aatcatggat acagaagaat atgcaccgca
    10741 acaaaatcct ggagcagatc aagcgattac ttcaggtaag atcgagtttc gcaatgtaag
    10801 cttttcttat gatggagagc acaatgtatt gaacaatatc tcttttgtcg ctaatccggg
    10861 tgagaccgtt gcattagtgg ggcacaccgg aagcggaaaa agctcgatca tcaacgtatt
    10921 gatgcggttt tacgaatttt atgaaggcca aatcttgatt gatgatcatg acattcgaga
    10981 atatccaatg gaagagttac gtaagaaaat gggattggtc ttgcaagatg ccttcttgtt
    11041 ttatggagat attgcaggaa atatccggat gatgaatcca gctatcacag acgaacagat
    11101 tcgccatgca gctgagtttg ttcaagcaaa caagttcatc gatgaattac caaaaggata
    11161 tcactcaaaa gtgatcgaac acggtgccag ctattctagt ggacaacgac agctgatcac
    11221 ttttgcgcga acaatcgtga cagatcctaa aatactagta ttggatgaag caacagcaaa
    11281 tatcgatact gaaaccgaag gattgatcca agaaggattg gcaaaaatgc gacaaggacg
    11341 gacaacgata gcgattgccc atcggctgtc cacgattcgg gatgcagaat tgattttagt
    11401 tttagacaaa ggtcatatag tagagcgtgg gaatcatgaa acacttctag cgcaaaatgg
    11461 tttgtatgca gatatgtaca aactgcaaag cagtggtgtg taaaaagagg ttgtgacagt
    11521 agcatagtag cattttgccc taagcgctag agtgaaaata aaacagagct tcgcagattc
    11581 ttcatctgtg agcgttagtg gcgtagtcac ccgatttaaa aaagacgacg acagctagtc
    11641 aaaagcggct aataaccact aagaaagcgt tgacaaaagc gtttgctttc gataatataa
    11701 agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc
    11761 atggttccat ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatatatc
    11821 atggaaagga gcaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca
    11881 gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag
    11941 tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc
    12001 tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt
    12061 gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat tctgtgtata
    12121 ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac
    12181 caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa
    12241 aaaacagtga agaaaatctc ggataaatac cacatcaagt tatcagaaga tgaagccgga
    12301 tttatcgcat tgcatttagt caatgcacaa ggcattcaaa gccaacaaga caatacctac
    12361 ctgttcacta taacaataca ggatattcta aatatcgtcc gctattacta taagattaca
    12421 tttgatgaag attcagtcca tttttttcga ttcgtcacac acttacgttt ttttctgaca
    12481 cgcgtcctag atggagaaaa atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt
    12541 attcaaaaaa aatataagga tacgtttggt tgcgttgaga aaatagcaga ctttttagcc
    12601 gagacgttca actaccagat gtccacggac gaaaaaatgt atttaacgat ccatactgcg
    12661 cgtttagtgc atgagaatcg ttgtaaataa ataaaaaaag gaggataaat taacaagaac
    12721 gtatgaaata agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga
    12781 atagaaatgg gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag
    12841 gaaaatatca atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa
    12901 gaaaaagcaa atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg
    12961 ggagggcagt atcaagttgt catcgggaat catgtgccag tcgtgtttga agaggttata
    13021 aaagcaggaa accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat
    13081 atgcttattg atggaatatc gggctgtttc caaccattcc ttgctatcct agctgctgac
    13141 gggatgattc gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct
    13201 ggcacatttg ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt
    13261 atcggtttaa cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc
    13321 gcagcattaa tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca
    13381 ctaacgacca tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc
    13441 attccctgga ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg
    13501 ttagctagcc aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc
    13561 ttcgttccat tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca
    13621 gtattcacat tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg
    13681 ccagttattt atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc
    13741 cattgggcaa tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt
    13801 atgacacctg ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt
    13861 aagcttagaa atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg
    13921 gggattacag agccagctat ttatgggttt acattgccac gtaaaaaaat atttattttt
    13981 acctgtgtcg gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg
    14041 aatcaaggtg gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta
    14101 acagatgtga ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttgcgctg
    14161 acttttttct tttggaagga ggaagcagat gaaacagatg atagacagaa aagatcaggt
    14221 aaagaaattg taaaaacacc gatacaagga caaatcgctc cactgaatgc agcaaaggat
    14281 gcagcttttg cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa
    14341 gtacgtgccc cttttgacgg aacagtcatg tccctgtttc cgacgaaaca tgcaatcggg
    14401 atggtttctg aagcaggtct ggaattgctg attcatgtcg gattggatac tgttcagttg
    14461 gaagggaaat attttgaaag cttggtgcag caaggagaaa gagtgaaaca aggcgatctg
    14521 ttgcttcgct tcgacaaaaa gaaaatcgaa gaggaaggat acagtttaga aacacctgtg
    14581 attgtcatca attacatgga ttatttggat attttggaaa atcaagggga aacggtcggg
    14641 ccagacgatg cacttatcac cgcactgacc tgaacggtta ggtgaaagaa tggactaggc
    14701 ttaactattc tttttagtcc acttctttat tttcaaaatt gaaaggctct ttcatgatgg
    14761 ctgagctgat agggcgttta tcaaattttt gtcctacaga aattagatgg actaaattct
    14821 gttgataatt ttctgcaaaa acacaactat ataacacgtc tagaagataa caaatcgatg
    14881 tattgatcgt aaaattacca attttcgaat acagtcgttc gcgtgtcgta agtcgcaaag
    14941 cggctgtact cattgaagat agcgttgaat caccgatact ggtcaaagct aaaattggca
    15001 catgattttg tttcaatatt tctgcggtac gaaggatcac attgttttca cccgtataag
    15061 aaatcagaat cccacacata tcacttgtgc aattgtacgc ttcatagaac gattcaccaa
    15121 aagtggtgac aatcgtgaca tccattttga tgcgcttcat tttcagcatg aaatcttgag
    15181 aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt ttttgcttga agcattagtt
    15241 gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag tgtatcttga atcgtcatct
    15301 gttctaaaga agccatttta tttgcgatcg tcataatacc gtctgtggtg gaaaaaggaa
    15361 gattcgcatc tacgtcttga aaattccctt caagataggc tgtttcagct aaaaaagcat
    15421 tacgaaaact gacccagcca tcaaaaccca ttttttttgc tacacggatg agtgtggacg
    15481 ggtggacata acatcgctca gcaatctctt ggatggtcat ttcgccaagt gctggagaat
    15541 cgcttaagag aaactgaatc acttcatttt ctgaaggaga aaaggagatt tctttgattt
    15601 tttcacgtaa taacatgtag ataccttctt cttgtttttt tacgtaaatc ataacttttt
    15661 tgaaaatgat ttgtaaacgg atttatgtta tatagcttaa taaagacatt ttttgacaag
    15721 atatttacaa aaatccagaa gaaaacacta acttttgtaa atgttactgg aaagaaacga
    15781 atttctattg tttgttttgt ttacaaaaaa gataataaaa gtgtaaaaag taaaggagtg
    15841 agaacatggc aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg
    15901 agttgatgga aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg
    15961 tagatgtaga agaaggaaaa gaaaagcttg agatcgtcag tcagatgtct cagcgtatgt
    16021 gggatgcttc tccgtatgaa gtcaaaatct atgcaactct ggatcgggaa gaagcattga
    16081 aagatgcgga ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag
    16141 acgagcggat ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga
    16201 tattcaaagc ttttcgtacc gttccagtgg ttttggcaat cgttgaagac atgaagcgtt
    16261 tatgtccaaa tgcttggctg attaatttcg cgaacccaag cgggatggtg acagaagcag
    16321 ttatcagata tggcaaatgg gataaagtga tcggattatg caatgtgcca gtaatggcga
    16381 tgatgacaga accaacaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg
    16441 gtctaaatca cttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag
    16501 aattgattga tcgtttatac gatgaagaca atggattgcc gaaaaatatc tttgacatcc
    16561 cgtttttcaa agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt
    16621 actatcgtga agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa
    16681 gagcgcagca agtgaaagaa acagaagcag agctgtttga attatacaaa gatccagaat
    16741 tggatcataa acctgaacaa ttgcaaaaac gaggtggtgc ttattattca gatgctgcct
    16801 gtgaaacgat tgcttcgatc tatggaaata aagaaacaca gatcgtggta tctacaaaga
    16861 atgatggagc tgttcctgat ctgccggcag attgtgtagt agaagtcact gcctatgttg
    16921 gcgcacaagg cgcacgaaat gtagcgtttg gttcccttcc tgctgctgaa agaggctggt
    16981 tgcaagtaat gaagaatatg gaattgctga cgatcgaagc agccgtgact ggtgattatg
    17041 gaacagcttt gcaagcgttt acgatcaatc cgcttatacg atcaggaaag acagcaaaac
    17101 ggatcatgga tgagttgttt attgcacatc agcagtattt accgcaattc aaggaaacaa
    17161 tcagccggtt aaaagcagaa ggaatcgaag tccaagatga actggcaaga gaattatcaa
    17221 gcaatacaca tgtaatggag gaagtatgat gggattcaga aaagattttt tatggggcgg
    17281 agctacagca gccaatcaat gcgagggcgg ttatgatgaa ggcggacgtg ggttagcgaa
    17341 cgtagatgtt gtgccaatcg gagaagaccg ttttcctgtc attactggaa aaaagaaaat
    17401 gtttgaattt gatgaaaatc atttttatcc agcaaaaatt gcgatcgata tgtatcatca
    17461 ttataaggaa gatattgctt tatttgcaga aatggggttc aagacttatc ggatgtccat
    17521 tgcttggagt cgtatttttc caatgggcga tgaaaaagag ccaaatgaat ctgggttgaa
    17581 attttatgag gatgtcttta aagaatgtca aaagtacggg atcgagccgc ttgtgacgat
    17641 tacacatttt gattgtccga tgcatttgat caaagaatat ggtgcgtggc gttccagaga
    17701 actcgttggt ttttatgaaa atctgtgccg agtcatcttc aatcgctata aaggacttgt
    17761 caaatattgg ttgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc
    17821 agggctgtat tttgaagaag gagaaaatga agaacaaatc aaatatcaag cggcacatca
    17881 tgagttggtt gcaagtgcca tcgcaacgaa gatcgctcat gaagtagatc cggagaacaa
    17941 agtaggctgt atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt
    18001 ttgggcttct agaaagtcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg
    18061 agaatatccc gcttatgcat tgaaagaaat ggaacgaaat ggcattcaga tcaaaatgga
    18121 acctggggat gccgcattgc tgaaagaaca tactgttgat tttatctcct tttcttatta
    18181 ttcttctcga gtttcgacta ctgacaacga actcttggaa cagactgcag gaaatatctt
    18241 tgcttctgtc aaaaatccat atctggaagc aagtgaatgg ggttggcaaa ttgatccatt
    18301 aggcttgaga ataacgatga atgatattta tgatcgttat caaaaaccac tatttattgt
    18361 agaaaacggc ttaggtgcag tcgatacgcc cgatgagaat ggttatgtgg aagatgatta
    18421 tcgaatcgac tatctggcag cacatatcca ggcaatgaaa gatgcagtag aattagatgg
    18481 agtcgatcta ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtacagg
    18541 tgagatgaag aaaaggtacg gattcattta tgtcgatcgt gacaacgaag gaaatggcac
    18601 attagcccgt tcgaagaaga aatcattcaa ctggtataaa aaagtaattg attcaaatgg
    18661 agaaaatctt tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt
    18721 tttcgagtat aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat
    18781 tggctgtaaa gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat
    18841 cgttattggc gcagggcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt
    18901 atccgtattg atgatcgaac aaggcgctcc tggcggccaa atgaacaata cggcagaaat
    18961 cgaaaactat cctggtttcg attcaatcat gggtcctgat ctagcttata agatgtatga
    19021 aggtgtttca agatttggga cagaaaatgc ttatggaatc gtgcaagata tcaaggatca
    19081 cggcagccac aaagaagtta tttgtgaaga taaaacttac gaaggaaaaa cagtcattat
    19141 cgcaacaggc tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg
    19201 tggcgtttct tactgtgcag tttgtgatgg tgcatttttc cgtaacaaac gtctagtagt
    19261 gatcggagga ggagactcag cagtagaaga agccatctat ttgacacaat ttgcttctga
    19321 agtagtcatc gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc
    19381 atttgcgaat gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa
    19441 tgaaatggta gtgacaggtg tgaaagcacg taatgtcaaa actggcaatg agtttgattt
    19501 ggcagcagac ggagtattca tctatgtagg attggatcca ttaactgcac catttagaga
    19561 agctgggttg acgaacgaag ccggctggat tccaacagac aacgaaatga agacgaatat
    19621 accaggcgtt tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactactgc
    19681 tgttggtgat ggtgggattg ctggacaaca agtgtataaa tacttggaat ctttagaaga
    19741 aacaactgca taagtttaaa aagagtgaaa gaacggtttc aattgttctt ttactctttt
    19801 ttttgcgtga aatctactga atccgattta tgcgaatact tgtaaccgtg cttgataaaa
    19861 gaatcttttc ttgatttaag cgttttttgg tcctcatact ttcatatcta ataagtgaat
    19921 gctatactta ttaagaacca tatttcagaa agaggtgtta ttttcatgtc ttgggaacaa
    19981 gtgtatgaac aatgggtaca tgaagaaaat ttagaagaaa acttaaaaaa acaattaaat
    20041 gatctgggtg aagatcctga aaaacttgaa gatgcttttt atgcaccgtt ggaattcggt
    20101 actgcaggca tgcggggaat cttaggacct gggatcaatc gaatgaacat ctatacggta
    20161 agacaagcaa cagaaggact tgcacgtttc atggatacac aaggtgaaga aacaaaacgc
    20221 cgaggtgtcg ctatcgctta tgactcacgc catcaatcac cagaatttgc catggaagca
    20281 gccaaaacat tagcacatca caatatccct tcttatgtat tcgaaagttt acgtccaaca
    20341 ccagagcttt cttttgcagt ccgtcatttg aagacgtttg cgggaatcat ggtaactgca
    20401 tcccacaatc cagcagctta caacggatat aaagtctacg gagaagatgg gggacaaatg
    20461 cctcctgcag acgcagatgc attgacgaaa tatgtccgtg aagtcagcaa tccgttaaaa
    20521 gttgaagtat tatctgacga cgaagctgaa catagcggat tgatcacgat catcggagaa
    20581 gaagtggatg ccgcttatct agaagaaatc aaagcagtaa cgattgatca tgaattggtg
    20641 gaaacaatgg gtaaagattt gaagcttgtg tacacaccac ttcacggaac aggaaaaatg
    20701 ttgggcgaac gtgcgttgaa acaagctgga ttcgaacaat tcgtactagt tccagaacaa
    20761 gctgtcgcag acccggattt cactacagtc aaatcaccga atccagaaga acactctgct
    20821 ttcgagtatg cgatgcgact tggcgagaaa gaaggcgctg acttactgat tgcaacggat
    20881 cctgatgcag accgtttagg cgcagccgtt cgtttgccaa atggctccta tgaagtattg
    20941 acaggaaacc aaatcggagc cctgtttgct caatatattt tggaagcaca ccaatcgcga
    21001 ggcactttac ctgaaaatgc agcggtattg aaatcaatcg tttctagcga attgcctaca
    21061 gcaattgcgg aacactatgg ggctaccatg ttcaatgtat tgacaggatt caaattcatt
    21121 gctgaaaaaa ttcaacaatt tgaagaagat cattcccata cttttatgtt cggattcgaa
    21181 gaaagttacg gttatttagt taaacctttc gttcgcgaca aagacgcgat tcaagcattg
    21241 ctcttattag cagaagtagc agcttactat aaaaaacaag gcaaaacatt gtatgacggc
    21301 ttgcaaacga tttttgaaac atatggctac tacgcagaaa aaacgatttc tgtgacaatg
    21361 agcggacaag aaggctctgc aaaaatctct gctttgatgg aaaaattccg tgaagaagca
    21421 ccgacagaat ttgccggagc aaaagtcatc caaacagaag acttcaaaga gttgacgaga
    21481 acagaccgtg aaggaaacgt agaaaaacta acgacaccgc catctgacgt attgaagtat
    21541 acgctggaag aaaatggctg gatcgctatc cgtccttcag gaactgagcc gaaaatcaaa
    21601 ttttatatcg gtgtaaaagg aaactcttca gaagacgcag ctgaaaaagt agccgcttat
    21661 gaagcgatta tcaaaaaaat gacagaagaa taaaaaaact tgagactgat ggaaaaagtg
    21721 gtaaacttta gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa
    21781 aaaacaaaag tagagaaagt cagccaacta ttcaaaatgt tgagtgaccc cactcgtctc
    21841 aaaattctgc tgtatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagta
    21901 gaaatggaac aatctgctgt atcgcatcaa ctgcgtttat taagagaaaa ccatgtcgtc
    21961 aagtcccatc gtgagggaaa agcgattttg tatagtcttg atgattctca cgtactggat
    22021 attttgaacc agacgctcaa acatgtggaa catcaataac gataggtgca aatagagaca
    22081 aactggactg tatcaacgaa tcttcttcgc tgacacagtc cagttttttt aagaaaaaga
    22141 caagaaaaac tgttagtctg tttcttcggt ggtttcagtt ccataataac tatgatcttc
    22201 taggtctaaa gcattcaaaa taagtgaagc tgtttcttcg atagataaag aagcgacatt
    22261 gattactacg cagcccaatt tttcataaag atcattcgca aaatcgagtt cagctcgtat
    22321 tttttcgata tcggaataag cagtatcagg atttaatccg taagagcgca tccgttcttt
    22381 acggatattg tttaaaatat ctgggttatt cgtcagtccc acgatttttt taggatcgat
    22441 ctcccaaagc tgtttgggaa tatgtgcttg tgggatcaaa ggtagattgg caacctttaa
    22501 atttttgtta gctaaaaaca agctcaaagg cgttttggaa gttctggata cgcctagcaa
    22561 aacgacatct gcttctagaa acccacgagg atctttcccg tcatcgtatt ttacagcaaa
    22621 ttccattgct ttgatccgtt tgaaatagtt ctcattcaat aaatgaaggg cgccaggttg
    22681 ccgaatgggc ttgataccag ttaagcgctc gatttctgat atcggcggag tcatcacatc
    22741 aaaactaaat aattgatgtt cttcacaaaa agaacgcgtc atctcgatca attcttgatt
    22801 gatcaatgtg tgtaggacaa gggctttttc ttttagtgca tcttgcagtg cctgcatcaa
    22861 tgttttctca tctgtcacaa atgttctgcg gaataaattg aactcaacgg taggatattg
    22921 ggccatagtg gcttgcgcta attttgtcgc agtttctcca gcagagtcag agatgacaaa
    22981 aaaagtaatg atttctgtag aatcggaaga catggataaa acctcacttg catttttttt
    23041 atgtttatta taccatactc tagaatgtgt acttcgtaat cattttgatt tggaggaata
    23101 agtcatgaaa aaacagacat tgattgaaca atcactagaa acattgaaaa ataacggttt
    23161 taaatatact aaaaaaagag aagcattgtt aacttattta gtgaagcgca atcgctacgt
    23221 atccgctaaa gaagtttttg actatatgaa tcaggaattc aaaggagtaa gttacgatac
    23281 gatttatcga aatctccatg attttgcaga aatcgattta ctggaagaaa ccgatttaaa
    23341 tggagagatg aagtttcgtt ttcattgctg tcaaggggaa atcggccatc accatcactt
    23401 tatctgtacg gtgtgcggaa caacgaaaga aatccatatg tgtccaatga attatttcga
    23461 agaacagctc ggtggatgta cgatcgaagg gcatagattc gagattttcg gaagatgtga
    23521 aaattgtcaa taaggattta aataaaataa catttgataa ttttaaaaaa aagcaagatt
    23581 tccttgacgg tcaaaacagc attagatata atatttacag gacagtcttg ttttttgaga
    23641 tatatttcaa taacaattac ggttactttt taagctcgga gggagggaat taacatgtca
    23701 aaaacagtcg ttcgcaaaaa cgaatcactt gacgatgctc ttcgtcgctt caaacgttcc
    23761 gtgtcaaaag cgggtacttt acaagaatct cgtaaacgtg aattctacga aaaaccaagt
    23821 gtaagacgca agaaaaaatc tgaagcagct agaaaacgca aaaaattcta gttctgtgat
    23881 gaattggagt tggtagtgtg tcactactaa gtagattaaa cgatgacatg aaaacagcga
    23941 tgaaagcgaa agataaggaa tctttacaag tcatccgaat gatcaagtca tctatacaaa
    24001 acgagcagat taaagaaggt cacgacttga cagaagaaga agaattaacg gtgttgtctc
    24061 gcgaaatgaa acagcgacgt gattctttgc atgagtttga agaagcagga cgtgacgatt
    24121 tagctgaaaa agtaaaaagt gaaattgtga tagtcgaaaa atacatgccg gaacaacttt
    24181 ccgacgaaga aattcgacaa cttgttcaag aagcaatcac acaaactggt gcttcatcaa
    24241 tgaaagaatt tggcaaagtc atgggtgcga tcatgcctaa agtcaaagga aaagcagatg
    24301 gaaaccaagt aaatgccatt gtgaaagaac tattacaatc ttaattttat catttaaagg
    24361 ctcttcgtca atgagtgttg gtgaagaaat tc
//
LOCUS       DAYYBG010000032        24274 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000032 DAYYBG010000000
VERSION     DAYYBG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.032"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACHAUN_001292"
     CDS             complement(55..789)
                     /locus_tag="ACHAUN_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8460002.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACHAUN_001293"
     CDS             1116..1526
                     /locus_tag="ACHAUN_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8460003.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACHAUN_001294"
     CDS             1519..2220
                     /locus_tag="ACHAUN_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8460004.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACHAUN_001295"
     CDS             2220..3806
                     /locus_tag="ACHAUN_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8460005.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACHAUN_001296"
     CDS             3825..4046
                     /locus_tag="ACHAUN_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8460006.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACHAUN_001297"
     CDS             4043..4804
                     /locus_tag="ACHAUN_001297"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8460007.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACHAUN_001298"
     CDS             5184..5693
                     /locus_tag="ACHAUN_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8460008.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACHAUN_001299"
     CDS             complement(5768..6307)
                     /locus_tag="ACHAUN_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8460009.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHAUN_001300"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHAUN_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8460010.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACHAUN_001301"
     CDS             7320..8171
                     /locus_tag="ACHAUN_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8460011.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACHAUN_001302"
     CDS             8199..8834
                     /locus_tag="ACHAUN_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8460012.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACHAUN_001303"
     CDS             8854..9627
                     /locus_tag="ACHAUN_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8460013.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACHAUN_001304"
     CDS             10035..10832
                     /locus_tag="ACHAUN_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8460014.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACHAUN_001305"
     CDS             10810..11223
                     /locus_tag="ACHAUN_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8460015.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACHAUN_001306"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACHAUN_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8460016.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACHAUN_001307"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACHAUN_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8460017.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACHAUN_001308"
     CDS             16000..16560
                     /locus_tag="ACHAUN_001308"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8460018.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHAUN_001309"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHAUN_001309"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8460019.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACHAUN_001310"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACHAUN_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8460020.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACHAUN_001311"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACHAUN_001311"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8460021.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHAUN_001312"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHAUN_001312"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8460022.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHAUN_001313"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHAUN_001313"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8460023.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACHAUN_001314"
     CDS             21907..22485
                     /locus_tag="ACHAUN_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8460024.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACHAUN_001315"
     CDS             22537..23019
                     /locus_tag="ACHAUN_001315"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8460025.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACHAUN_001316"
     CDS             23136..23510
                     /locus_tag="ACHAUN_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460026.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23885..>24274)
                     /locus_tag="ACHAUN_001317"
     CDS             complement(23885..>24274)
                     /locus_tag="ACHAUN_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8460027.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         7906 a         4353 c         5198 g         6817 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgggagcgt caataatttt gtgtaaataa attgtcctcc tgcaaaataa
    23881 ttagttactc agtaaacatt gaaactaatg tatcggttac ctgttgaaaa cctttatggc
    23941 ttctgtttag aaatttttga ttgtatgtat caaaaatgct gactagaaag cgttctagtg
    24001 attcttcatt ttgaaactgc tcttttctac ggctgtatct tttaatttgc ttattgaaag
    24061 actcgattag attggttgag taaatggttc tacgaatgct aggtggaaaa tcataaaaag
    24121 ttaataagtc ttggttttct atgagtgact gcgtcacttt aggatagttt ttcttccatt
    24181 tctcaatcat gccggataag aaggtattcg cttcttcttt tgagttagct tgataaacag
    24241 ccttaaagtc atcacagatt tcttttcggt cttt
//
LOCUS       DAYYBG010000033        24258 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000033 DAYYBG010000000
VERSION     DAYYBG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.033"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACHAUN_001318"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACHAUN_001318"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8460028.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACHAUN_001319"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACHAUN_001319"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8460029.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACHAUN_001320"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACHAUN_001320"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8460030.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="ACHAUN_001321"
     CDS             complement(3374..4681)
                     /locus_tag="ACHAUN_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8460031.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="ACHAUN_001322"
     CDS             complement(4684..5265)
                     /locus_tag="ACHAUN_001322"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8460032.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="ACHAUN_001323"
     CDS             complement(5837..7129)
                     /locus_tag="ACHAUN_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8460033.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="ACHAUN_001324"
     CDS             complement(7126..7749)
                     /locus_tag="ACHAUN_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460034.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="ACHAUN_001325"
     CDS             complement(7752..8588)
                     /locus_tag="ACHAUN_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8460035.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="ACHAUN_001326"
     CDS             complement(8605..9492)
                     /locus_tag="ACHAUN_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8460036.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="ACHAUN_001327"
     CDS             complement(9508..10791)
                     /locus_tag="ACHAUN_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8460037.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="ACHAUN_001328"
     CDS             complement(11474..12157)
                     /locus_tag="ACHAUN_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8460038.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="ACHAUN_001329"
     CDS             complement(12175..12792)
                     /locus_tag="ACHAUN_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8460039.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="ACHAUN_001330"
     CDS             complement(12802..13626)
                     /locus_tag="ACHAUN_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8460040.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="ACHAUN_001331"
     CDS             complement(13619..14179)
                     /locus_tag="ACHAUN_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460041.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="ACHAUN_001332"
     CDS             14353..15309
                     /locus_tag="ACHAUN_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8460042.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="ACHAUN_001333"
     CDS             complement(15614..16570)
                     /locus_tag="ACHAUN_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8460043.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="ACHAUN_001334"
     CDS             16674..17276
                     /locus_tag="ACHAUN_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8460044.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="ACHAUN_001335"
     CDS             complement(17364..18863)
                     /locus_tag="ACHAUN_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8460045.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="ACHAUN_001336"
     CDS             complement(18879..19403)
                     /locus_tag="ACHAUN_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8460046.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="ACHAUN_001337"
     CDS             19701..20450
                     /locus_tag="ACHAUN_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8460047.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="ACHAUN_001338"
     CDS             20441..21226
                     /locus_tag="ACHAUN_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8460048.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="ACHAUN_001339"
     CDS             21195..21911
                     /locus_tag="ACHAUN_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8460049.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="ACHAUN_001340"
     CDS             complement(22013..23032)
                     /locus_tag="ACHAUN_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8460050.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..24052)
                     /locus_tag="ACHAUN_001341"
     CDS             complement(23051..24052)
                     /locus_tag="ACHAUN_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8460051.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7189 a         5222 c         4224 g         7623 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcgactga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatt
//
LOCUS       DAYYBG010000034        24248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000034 DAYYBG010000000
VERSION     DAYYBG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.034"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..940
                     /locus_tag="ACHAUN_001342"
     CDS             65..940
                     /locus_tag="ACHAUN_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8460052.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1085..1237)
                     /locus_tag="ACHAUN_001343"
                     /pseudo
     CDS             complement(1085..1237)
                     /locus_tag="ACHAUN_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1505..2836)
                     /locus_tag="ACHAUN_001344"
     CDS             complement(1505..2836)
                     /locus_tag="ACHAUN_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8460053.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(2985..3698)
                     /locus_tag="ACHAUN_001345"
     CDS             complement(2985..3698)
                     /locus_tag="ACHAUN_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8460054.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            3873..5297
                     /locus_tag="ACHAUN_001346"
     CDS             3873..5297
                     /locus_tag="ACHAUN_001346"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8460055.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5596..6303
                     /locus_tag="ACHAUN_001347"
     CDS             5596..6303
                     /locus_tag="ACHAUN_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8460056.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6290..6613
                     /locus_tag="ACHAUN_001348"
     CDS             6290..6613
                     /locus_tag="ACHAUN_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8460057.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6702..7739)
                     /locus_tag="ACHAUN_001349"
     CDS             complement(6702..7739)
                     /locus_tag="ACHAUN_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8460058.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8060..9043)
                     /locus_tag="ACHAUN_001350"
     CDS             complement(8060..9043)
                     /locus_tag="ACHAUN_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8460059.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9458..10228)
                     /locus_tag="ACHAUN_001351"
     CDS             complement(9458..10228)
                     /locus_tag="ACHAUN_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8460060.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10221..11156)
                     /locus_tag="ACHAUN_001352"
     CDS             complement(10221..11156)
                     /locus_tag="ACHAUN_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460061.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11472..13121)
                     /locus_tag="ACHAUN_001353"
     CDS             complement(11472..13121)
                     /locus_tag="ACHAUN_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8460062.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13238..15841)
                     /locus_tag="ACHAUN_001354"
     CDS             complement(13238..15841)
                     /locus_tag="ACHAUN_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8460063.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15853..16902)
                     /locus_tag="ACHAUN_001355"
     CDS             complement(15853..16902)
                     /locus_tag="ACHAUN_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8460064.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(16928..17242)
                     /locus_tag="ACHAUN_001356"
     CDS             complement(16928..17242)
                     /locus_tag="ACHAUN_001356"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8460065.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17258..17704)
                     /locus_tag="ACHAUN_001357"
     CDS             complement(17258..17704)
                     /locus_tag="ACHAUN_001357"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8460066.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17705..19627)
                     /locus_tag="ACHAUN_001358"
     CDS             complement(17705..19627)
                     /locus_tag="ACHAUN_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8460067.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19839..20735)
                     /locus_tag="ACHAUN_001359"
     CDS             complement(19839..20735)
                     /locus_tag="ACHAUN_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8460068.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20728..21408)
                     /locus_tag="ACHAUN_001360"
     CDS             complement(20728..21408)
                     /locus_tag="ACHAUN_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8460069.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21409..22191)
                     /locus_tag="ACHAUN_001361"
     CDS             complement(21409..22191)
                     /locus_tag="ACHAUN_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8460070.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22184..23101)
                     /locus_tag="ACHAUN_001362"
     CDS             complement(22184..23101)
                     /locus_tag="ACHAUN_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8460071.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23265..23519)
                     /locus_tag="ACHAUN_001363"
     CDS             complement(23265..23519)
                     /locus_tag="ACHAUN_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460072.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         7349 a         4910 c         3992 g         7997 t
ORIGIN      
        1 tacttttttc ttaaacgttt ctttatacta aatataaata agtgtttcat gaaaggagaa
       61 ctagatgaat aattggcttg agtattttcc tgaaaacgtt ttggaacgcg gttacagcta
      121 tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt ccgcaacagt
      181 ttctggcacg gaagattata aggtagtgat cacttgggat gaaaagacca acatgacttg
      241 tgattgtcta tatgccatag aaggaaaaaa atgcaaacac atggctgctg tcttattcgc
      301 ttacgaagaa cgtccaataa aaaaatccaa ctactcactt tccgaactct cgtcccttgt
      361 ttcctccgct agttcctctc ttgtacggga gcttttaaca gagattctta tagaacatcc
      421 gcaatttatc gaacgcttca aagttaagat gccatttcat gcaataaatt attcagataa
      481 attaacaact atcatccata aatacgatca tattatcaag aaaaacaaaa acaggaaaac
      541 agcgaagttc atcatggaga tgcgaaagtt catccaagaa gcagttgaat cgctcattca
      601 gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa gtgattgcta ctctcgaaac
      661 attctattgg gagcctgagg atgaacgaac ccttcttctg atagaggact gttattattt
      721 atggaaagaa ttattagcag aagcacctca tgcagaaaaa aggcaaatgt tctcttggtt
      781 cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa cgctactcca taaaaatcct
      841 gaaagaagac tttcgggaaa aagaattttc taaccaaaag aaaaaaatag ataaaaaaac
      901 taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg ataacaacca
      961 tcctaaatcc acaagaaata cagcagacct tgctttaaaa acacgaatca tgcaaaggat
     1021 gaagtaaaga agacttaacg cgtacccaat attagtcatt tgactattag gtacgcgttt
     1081 tgttttataa gtcatgacaa atttccttcg ttgctgggta gattttccgg cattgttgat
     1141 agattttatg atacttttct acattttgta agagaggttc cactgcatct gtatattgga
     1201 taaaattctg acaacaactt tcaggtgtgt cgaaccactc taatctttaa agataaatat
     1261 taaaataacg attaataata ttaacagtct ttatttttca ccaccaaaaa cctatctttg
     1321 catatactaa atagacgtat atacacagat aggtttgtct tgtgaaaaat aatgattatc
     1381 cttaccaaaa aatgtttgtt tttctacatg ttcgtactta tcaaaaaata tgatccttat
     1441 tgaaattact ggctatttat attccaacga cttcttgtgt taatttttgt tatctcatag
     1501 taaattatgt ttcattttca cttaatttag ttgtcacgtg ttcgtcaatt cgtaaaaacg
     1561 gtaaatatat aaacacactt gcggctataa ttaatatttg taaaacagct gctcgccagt
     1621 ctccggctgt tgctaaaaat cccgatataa ctggtggcgt agtccaagga atttgcacta
     1681 ctacatgatt aattaatccc acagcagttg caaaatatgc tactgtcaaa gaaaaaatcg
     1741 gtgcaagcac aaagggaata atcagactga tattaaatac aataggcaat ccaaaaataa
     1801 taggttcgct tatattgaat atagcgggag ttaccgataa tttagcaaag tccttataat
     1861 cttttctacg actgaagata aatattgcaa tcaataaagc aatcgtattt cccgatccac
     1921 ccattaccgc aaaggtatct ttaaaggcca tattgataat atgtggaatt tgctctttgt
     1981 ttgcataagc tgccatattt tcttgcatat tctgaatcaa aaacggatct aacaatgaac
     2041 ctgatattac tgcttgatgg ataccaaatc caaaaaatag attagcaata gatgtaatta
     2101 acaaaaatcc tggtaaacca gttgtaattt tacttaatgg ctctgtaact atcgtggtaa
     2161 ttagcttatt tatatccata ttaaacaatt gaatgattat aaatgacaat aaagcaaacg
     2221 aggtaatcgt aataatgatt gggattaata cattaaaaga ctttaaaaca gcgggaggaa
     2281 tatttccgga tagctgaatc tgtagtttct tattattaga aagtttaata aataattctg
     2341 tagccgctaa tccaacaaat atacctacaa acattcctga agctcctgta taggatatag
     2401 gtatcacacc cgtcacttca acagctttat ccgcattttc aggagtaaac atcatctgca
     2461 taggtgttaa tacaacaaat gtcgaaatag caactaatac cgctgcaata tgatttgtat
     2521 aatttctgct tgcacacata tgataagata ctgctgctac aactaataat gtagttatgc
     2581 ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa cgtggtcaat gtactagtat
     2641 cgacaatttc ccccatgaat cctgttggtt ctaagatcac tgaattaata agtgtcacaa
     2701 atcctgctaa catcataaat ggcataaatg tagcaaacgc atctcgcaaa gttcttaaat
     2761 ggatctgatt ccccaactta gtcgcaacta taccaaattt ttcaaataca atgtccttcc
     2821 tgctactttt gcccatattt tttctccttt atgcttattg taatcgatta ctttttccac
     2881 ccaaataata catcacttat caaaaaaagt aaatactttt aattaaatat atatatcttt
     2941 tttagtataa aaaaataagc tctgaaaaaa taattttccc gagcttattt ttcaacaaaa
     3001 acagcttcaa aaacaaagtc ttgatataaa tgtctcgata ccgaatactc gaaaggttta
     3061 ccagacgata gagcaacaac tttgtctatc tcaatcataa attcacctgg atctattttt
     3121 aatatttttg cgtcaaattc attcgcagta tctcctttga tacgtatgtg cgaagtcttt
     3181 atttccagac ccagttcatt tgtgatataa gcatatacag atttctttaa atgttttggt
     3241 tccaagcctg gtatgactgc taatggcatg aatgtttgct caatcgagta tggttgacaa
     3301 tcaataaaac ggcttcgtac aatattatat acatagtctt ttacactgat acttaattta
     3361 tcagctactt ttttcgatgg aagttcaatt gagaaagtat ggatttctga atctacgtgt
     3421 tcttcaccaa ccacgctaaa caaaccttcg tttggaccaa gaactcgtga gttatcaatt
     3481 ggtgttctga cgacttcagt cccataccca cttctacttc gcaaaactcc ttcggaaaca
     3541 agtaagtcta cgcctttctt tactgttaac ttgcttgtat gataaataac tgccaattca
     3601 tctcctgtag gaagtttctc accaatttta tactctccat ttacaatttt tttctttata
     3661 tctaagtaaa tttccatata cttcgcaatt tttttcaatg gtgaccctcc aatcaaaaac
     3721 atatcttcat ctatacttta acataaataa accgataaaa tccacatcaa aattatacac
     3781 acaataaaaa agatatatat atttaaacaa aactatttac tttaagttgt aagcggtgtt
     3841 atcattaagt ggaaacagga ggtaaaaaaa ttatgacaac tatgaaaaag ctaacttttc
     3901 cagatgattt ttggtggggt tccgcttggt cagctgagca agcagaagga aaaggagaaa
     3961 caggaaaagc agagactatc tgggatcgct ggtttaaaga acaaccttac cggttttatc
     4021 aacgaatcga ttcctctatt acaaccgatc acatccatca atataaagaa gatatagaac
     4081 taatgaaaaa aactgggcat aactcctttc gcgtttctat ttcttgggca cgaatgtttc
     4141 caaatggcgg taccggaaaa gtaaatccca aagccatttc tttctatcga aaattatttt
     4201 ccgaaatgaa taataacgga atcaaagtat ttgccaactt atatcatttt gacatgcctg
     4261 ctactcttca agataaagga ggatgggaat cacgtgaagt agttgatgct tatgttcatt
     4321 acgctgaaac ttgttttaaa gaatttggtg acttagtttg tcattggttt accttcaatg
     4381 aaccacttgg gcctatttta ggtacttatc ttgaagattt tcattatcca aacctcattg
     4441 actttaaacg cggagcgcaa gcagcattct tcactatact tgcacacgca aaagcaatac
     4501 aagtatttaa agaactagct cttcctagta aaataggcgt tattctaaat ttgagtccta
     4561 cttttcctcg tagtcaaaat cctgctgatg tgaaagcagc agaaatcgct gatttatttt
     4621 atacgcggag ctttttagat cctatggtta aagggaattt ttctaaaaag cttatcctgt
     4681 tattacaaga atataatcag cttcctgaag attatacgaa agaagatctt gtatgtatct
     4741 acgaaaatac cgctcaaatt cttggactaa actattacga gccacgtcgc gtcaaatctc
     4801 gttcttctat ggtggataca tctagtccat ttttaccaga gtggtttttc gaaccacata
     4861 tcatgcctgg acgacgaatg aacgaatacc ggggatggga aatttacgaa aaaggaattt
     4921 atgatttgtg catggatatc aagaaccatt atggaaacat cgaagcattt atctcggaga
     4981 acggaatggg cgttgcggat gaagaacgtt tcatggatga tacagggcaa ataattgatg
     5041 attatcggat tgagtatatc aaagatcatc ttgcttactt atggaaggca ataaatgaag
     5101 gatgtaacat taaggggtat catctttgga catttattga ttgctggtca tggatcaacg
     5161 cctataaaaa ccgatatggg ctggtttcac tcgaccttac tactcaaaaa agaacaatta
     5221 aaaaaagtgg cgaattttac aagcaattaa gtgatacaaa tggatttaga tacgatgtag
     5281 acttgttagt tgaataatat tctaaaattc cacttgaagt gaacacatta gaaaacaaga
     5341 ataatgagaa ataccaataa caaatacttt ttcttcatac ttcatttata cttgtcagta
     5401 taagagactg tgacaaatgt agtgatcctc agaagttagg tctggaaatc taacttgaga
     5461 agattttttt aaactacgtc cagtcttttt tatttgttta aaattgaagt tgacaagtct
     5521 tcattaaaaa tttagaatat tcacaagttt gacgacaatt atgaaaattt atgtcgtgct
     5581 aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa agacacgtta
     5641 cctaccgttt ttggttatat cggtattgga cttgcatttg gtatcgttgg gaaagctgcc
     5701 gggtttcatc cgctggtcgt cacgttgatg tccctgctga tctatgctgg ttctgcccaa
     5761 tttatcacgg tcagcatgct tgctagccat agtccactgc tctccatcgt tctctcgacc
     5821 tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta tttcaagaaa
     5881 aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag tttcgcactg
     5941 ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat gaatacagct
     6001 aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact tttagggaat
     6061 tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat gtttatcggg
     6121 ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca gttgatcctg
     6181 attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag taacttagtg
     6241 attttagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca tgccttcttt
     6301 tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt ctaggatcct
     6361 accatttgtc ttactgaaaa aattcgattt gccgcaaccg ttgcttgagt atttaagttt
     6421 tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc aaaatatcgg
     6481 acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac ttttagctgc
     6541 tattctttca aaaagcttat tggtgatcgt cttggtagga attctttccc tttcgttgat
     6601 tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg cgctcttgtt
     6661 tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt gaacggacca
     6721 ctaacaacga cttttcctaa tatatgtcct gtttctacgt ctttggttgc ttgtttcaag
     6781 ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat aatcccttca
     6841 tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc atagttatag
     6901 tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt ccaaacacct
     6961 atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg tcggacaagg
     7021 tgtttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata ctctacttga
     7081 tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat tggatagtct
     7141 acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta gacttctagg
     7201 ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccagctcc gttgatcact
     7261 aagatttttt tcccgcggtt ggcattttct tctggagtca gaccaaattt ttcaaataac
     7321 aattcataag cggtcaatga ggtcaacggc aaggctgcgg cttcttcatc tgaaagattt
     7381 tttggtgcaa gagcgacaat acgttcgtca accagttggt attcttgatt gcttcctgct
     7441 cttgtcgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg cacttcatca
     7501 cctacagcaa caactttccc gactccatca aaccctaaga tacgtaaagc atttcttatc
     7561 ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt gacttttacc
     7621 agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa gctttttgga
     7681 tcatcgatcg acaacccttc ataaaaaccg actaccttca ttaattcctt ttctgacata
     7741 cgaattcctc cttagcttca tcaagttgga gcatccatac agttttcatt tcaatatata
     7801 tagtatgcta tttgttccac ttttattcat cataattatt ctcttactta aagtaaacta
     7861 tttgcacttt tttggttaaa aaagaggttg cgacaagact tttgtcacaa cctcttttaa
     7921 acggtgttca atcaactgac cctcggtatt cgctcaaaaa acgacaaaat atgaggagct
     7981 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacga cctctaattc
     8041 acggtgttca agcaagaaat cattgttcac tgaaaagcgt tggatgttct ttttcaaagg
     8101 tgtctaagat ctctcgtact tcatcagtag atttcgtatt catcaactga actcgcaagt
     8161 catttgctcc tggaaatcct ttgatataga ttttaaagaa acgatgtagt cctgtgatgg
     8221 aacgcgggat gatttctgaa taatgatctt gcagatctag ttgcagacga agtagtccga
     8281 tcagttcttt ttctgtatgt tccctcggtt ctttttcaaa tgcataagga ttcttgaaaa
     8341 tcccccggcc aatcatgatg ccatctacgc catatttttc ggccaattct tctcctgtct
     8401 ggcgatctgg gatatctccg ttgatcgtga tcagtgtttg cggagcgatt tggtcacgta
     8461 aagcaatgat tttagggatg acttcccaat gtgcatctac tttactcatc tctttacgcg
     8521 tacgcaaatg gacagaaaga ttggctatat cttgatgaag taggtgactg atccattctt
     8581 ccatctcaga catttccgta tagccgatac gcgtcttgac gctgacaggc aaaccgccag
     8641 ctttagccgc ttcaatcaat tgtgccgcta cttctgggcg aaggatcaag ccactgcctt
     8701 ttccacgttc tgctacatta gggactgggc agcccatgtt gatatcgatt ccttggaagc
     8761 ccatttctgc catagcaatg ctcatttctc taaaaaattc tggcttgtct ccccaaatat
     8821 gggcaaccat cggctgttca tcttctgtaa aggtcaatct tcctcgtaca ctgtcttttc
     8881 catctggatg acagaaactg tctgagtttg taaactcagt aaagaagaca tccggtgctg
     8941 ctgcatgctt tacgacatgg cgaaatacaa catctgttac atcttccatt ggtgctaaaa
     9001 caaaaaaagg ctttggcagc tctttccaaa aattagtagt catttatttg ttactctcca
     9061 ttctatcaag cagtgaagtc gcaatctttc tcaaaaacat tgtcattata ctaggaaaca
     9121 aaaaaagaag caaagtgaac agcttaaaag atgtttctga ttgagcaaaa aaagactgtg
     9181 acaaaaatct tatcacaatt ccttttccgg aataaatggt gttcaaaagc ttgcatctgc
     9241 ggtaataagt ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
     9301 acttgatgca gaacagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc
     9361 ctcggtatta gctcaacaaa cgacaaaata tgaggaacta ttttcccgtt tgtttctcta
     9421 atactcaggt caaaacgctg atttcacaac ctcttttcta acttctcaat cctcttcctt
     9481 tattgatcaa ataccaagta acggcgtaca aaacaacgat gaatagcaca agtacgatca
     9541 tcgatgttgc tactggcaca tcgattgttc caaggaatcc gtaacggaaa ccggagatca
     9601 tataaacgat cggattgact ttcgaaacag cttgccaaaa tggcggcaac atagagatgg
     9661 catagaaaac gccacctaga taagttaacg gctgcaagac aaatgtaggc acgatcgata
     9721 catcgtcata agactgagca aaaatcccgt tgagcagtcc tgctaacgaa aacaagattg
     9781 ccgtcatcaa taatgtaatg atgaccatga accatgaata cacatgaagt ggtacaaaga
     9841 acaaggaaat gatcgttacg agagagccga cgagaatact gcgacccact ccgccaatca
     9901 caaagcccca aatgatcaca tgcgtaggaa caggcgctac caagatttct tcgatgtttt
     9961 tctggaattt ttgagagaag aaggacgaag aaacgttggc atacgaacta gtgatagcag
    10021 acatcataat caaaccaggc acgatgaatt ccatatatga aaaaccaccc atgtcaccga
    10081 tccgaccacc gatcattttt ccgaaaataa caaagtacaa ggatgtcgtg atcacaggcg
    10141 gaaccagtgt ttggacccag atacgcaaat agcggttcgt ttccttgata gctaaacttt
    10201 ttaatgcagt aaaataaaga ttaaacattt ttctcctccg tatgattatt ttcttctgtg
    10261 attttcaaaa acagttcttc taaacgattc gacttattcc tcatagaaag aactttgatt
    10321 ccttgtcttg tcagctgatc gaagattccg ttcacccctt gatttcgttc aacttcgaca
    10381 gcgattgtct gttcatcttc aaatgtacaa gcgtatcctt caatcacagg tttggtctca
    10441 tatggcgcta aatcaaagat aaatgtttca aattgcagtt tggatagaag ctctttcata
    10501 ctagtattct cgatcagttc acctgattgg atgatcccga tgttgcgaca aagcatttca
    10561 gcttcttcta aataatgcgt cgtcaaaatg atcgttgtac cattttcgtt caattctcgg
    10621 agaaactccc acatctcgcg tctcaattca atatcgactc ctgctgttgg ttcatccaag
    10681 atcagcaaac gtggttcatg catcaatgcc ctagcaatca tcagtcttcg cttcattccc
    10741 ccggaaagca tacgtgcacg gacatttctc ttttcccaaa gattggactg cttcaaatat
    10801 ttttcgctgc gtttcatcgc ttctttgcga gagacaccgt agtagcctgc ttgattcacg
    10861 acgatttgct ggaccgtttc aaagggattg aaattgaact cttgcgggac taatccaatc
    10921 tgttgctttg ctcggactag atccgtatcc agatcataac cgaagacatt cacttttcca
    10981 gatgttttat tgactaaaga agtaatgatt ccgatcgttg tagacttccc agctccattt
    11041 ggtcctaata gcgcatagaa atctccttct ttcacggaaa gatcgattcc tcgcaatgct
    11101 tcgaccccac ttccataaac ctttttcaaa ttatttattt caagcgcatt tgccatttat
    11161 ttcctatctc cttatagaca tgattcttta ttgtatcatg gaatacagca gatgaaaaaa
    11221 caacttggtt ccagcgaaaa atcaggttgc tgggctcagg aaaatctcct aatgaaagac
    11281 atcaatcgga cgaagtcaga caaccttttc agaaaaaaca aatcgaagat aaaggattac
    11341 tattcatatg tgcaacaaaa ggagcgtgag acaaaagtaa agaacacttt tgtctcacgc
    11401 tctaaacacg tataaacggc ggaagcaaaa gccaagattt ttgatccaat aaatactttc
    11461 gatcacttcg cctatcccaa tacatgccac tgccgctttt catcaaataa cgcggcttcc
    11521 gtttttgcgg tttctactaa agtctctggg taatccaaaa ctttcagcaa ggcaatcgca
    11581 tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa aactgattcc ttttccttct
    11641 gttacttgtt cttcaaagtg gacattctca caataattct ttaaaatctc tgtcaattcg
    11701 atatcatgtg tggcgacaaa tgctaaagat ggatactccg caagccaccg aacgactgaa
    11761 gcggaagaag caatccgttc gatcgtattt gtccctttta aaatctcatc tacgaagcag
    11821 tagcagcgtt ctttggaagc cacttggtcc aacaaacgtt tgatcgattt gatttctgaa
    11881 acaaaataac tgtcgccttc aaataaatca tcttcgattg ccatggaagt caacacatgt
    11941 ccaggttgca gggtaaactg ttctgcaatc gctgtttgga tcgtctgtgc tagaatacag
    12001 ctgatggcta tacttttgac atacgtagat tttcctgatg cattggaacc cgtgactaat
    12061 gtattcttct gccagttgac cggattcact accgcttctt ttaacaatgg atgatagata
    12121 tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc aagtgatggg catataagta
    12181 cggtaattca atattgcagc ggctacttct aattctccca gcaattccca cagcttgatg
    12241 gcttcagttg aatgcttttc taatttagtg agaacgaaat gataggagat aaatggcagc
    12301 atgaacatga tgttgacata ttcaaaaatc aactctgctt cactcccact cttcatacga
    12361 aatgaaaaac ctaatttagg gatgtctctt aatggtttag tttgctgttt cagctcttct
    12421 tgaagtggtg tcggcagttt tgctagctga ttggcggctg aaatcgttga aaccaaataa
    12481 ctcatgctat tcagctctgt ttctagtgtt tgtttcttca ctagataata gaccgtattg
    12541 aatactaacc caccgattgc caaatagatt cctagtactt gtcctaaaag aagcaaaccc
    12601 aaacccacca gcggaagcaa gccaagaaaa ataaataaac ctacgcgtcc aatcttttta
    12661 ctttttccat ccgctagata ttgttttgca aaattatgat ctttttttcc taaacgagcg
    12721 aattgatatt ggattttttc tctcagttga ggattttcct gataaaaagc aatcagtttt
    12781 tctaactgct gatcttcacc aaaatcaaag ctgcgtaaac gttgatacaa cgcttctgaa
    12841 cctacactag aataggtact gttgattcct tcgaatactt cgaacatatc caggtcatac
    12901 caagtaagat catcgatttc gctatcccat gaacggaact tcttctccgt gagccatgct
    12961 tccttcaagc tttcttcttt atcaaatctc ggttgataag gaattttttc ccatttgcta
    13021 cgaactgttt cctttaattt caaacgtgaa aaaatgtcta aagcaactaa taaaataaca
    13081 gctgcaataa taattaaaac gataaattct ctttgaccca tatccctctc cgttccttta
    13141 tttgttagct ttagtatagc actgttgcgc tatttgaaaa gagggtaaaa aaacaccttt
    13201 caaaataaaa ggtgtttttt tcttgtatat tacatattta tctctcattt atcaaaattg
    13261 acaaataatc atagggagga attgttgtta attcttgttt attctccagt gcattgacaa
    13321 ctgtcccaag ggaaaggata gcctcaggta ctattttttc ttgttctgtt ggattagcta
    13381 agcgaagaac aaatccttgt tcatgaaaat aagaaggata aatcgatgat acaattaagt
    13441 cttcaggtaa ttcaatcaat gaaaatttac gaggaacatc aagttcattt tgtaatggcc
    13501 aaatcttgtt atctaacctg tgaataaata cattcagttt ctgcttttga taagaaatag
    13561 attgttctaa ccgtttttct gcctgttttg ccactttgaa ctcattaaat cttccttctt
    13621 ctacattaaa tccaaaagtg actttatact cacctatctc ttgagctagc ggtgtttcca
    13681 tcatgacatg cccttcattt gtcgtatctc ctgaagctcg cccaggtcgc caagcaagat
    13741 taggttttcc aagttcccct gttgttgaaa ataaggttat gattaattga ttttctatac
    13801 gttcatactc ttttaaacca tctgcataaa acgtaaaata atgatcattt tctgccacag
    13861 agacactctt atcgaatatt tctaaattaa caggtttctc taccatagtt tctgaccaat
    13921 tcggaggaat gacatcaggg gtattttcaa taaatccgtt ttgaatttgt gcaattgatt
    13981 tttcagattt atctaaaaca ttaattgcta agcgcatacg atgcgagtaa acagtattat
    14041 gaattttgag tgtgccttca acgatttgac tttctcccag actgaacgat atttcataag
    14101 atatttcttc catttcgggt ttttcagata aacgatcttt taaatcttta ggaagttgag
    14161 cttttccgta aacaactaat gtttcttgta aagaatcttt atatacttta gctgtttcta
    14221 attttagaga tagttctgta tctccttcta atggtgaata atcatacgta tcgccatcat
    14281 ttgctgaatc gatcaaatga acgaaatcaa gatattgtct atctccaact atcaaattca
    14341 attgcccatc tttaaaagaa agtttatatt tattgttact tatctcagtt ttgtctatct
    14401 cgattgctgt ttctaatttt tcattatttt tctcaaattc aacaacagtg tatcctagtg
    14461 ctggcacatt tacatttatt tttatgtcaa gttcataata agctggttca tcaaaatatt
    14521 cgaaacctgt agcaatcatc cgataagcat ttttccgttc tggaaaataa acttcttttt
    14581 caatgatatt atagggacta cctttgaagt gtattttctt tgaatcagct actacatgca
    14641 cgattttcga tccactataa ttctcagtcg ttgtgttgaa aacaaggact tcattcgatt
    14701 ttagctttaa tgcatcagcc atccgtttta caataaggtt ttcgattccg tcagctatct
    14761 cattagcttc tttgattcga tggaaaatat cttccgcaac attatctgaa acacagccac
    14821 caatactatc atgagcctgg ttttgcaaaa tctttttcca taaccgacga agtaggcctt
    14881 tactgatatg gataccattt ctttctgcaa tcagcatgag cggctcaact cgttttagaa
    14941 ttttttgttc caatttaaaa ttttctattt tcatttttgt tcttactgag ccaatagaac
    15001 gatgaacacg agagtaaacc ggctgacgaa gttcaccttt atattcaggt aattcttttt
    15061 tgtttctgat aacttctaca taagaaggat aatctgaaat aatattgtgg aatttactgt
    15121 atttattcaa cttctctacg acttgatcaa aatttaaaat cattgacttt tgatctacac
    15181 ccgaaggtac taaaatatct ttgttttttc cttgcatatg aagcatttca actgcttcat
    15241 ccaacttttc ttttgcaaat tcaggaatat ctttaattgc gtctaaggtc atcattccag
    15301 tcatataacc aaatggaaaa ttcatggcat aaacaaattt atctcccaat cctttccaga
    15361 taaaataagg agatggaacc atcgtttcaa agtttaatcc tcgccaacac atgaaattat
    15421 cgatttctac ttgatgcaaa agtgtaggca aattcgcatt gaacccaaac gtgtcaggca
    15481 aataccctac catcattgga tctccatatt taaggatcgt atcactgatt ccaatcatta
    15541 agtttcttaa aattgattca gcatccacta ataaggcatc tgtttgtgca taccacggtc
    15601 ccacgaatag ttgtcctgtt ttcatcagtc gttggataac cttaagcttt tgcggattga
    15661 tctccaaata ctcatcgacg attgaagttt gtccatctaa acaaaagttt actttagggt
    15721 tcctttccaa ttcatctaga acttcagtaa agacctgatc agacaatacg atggcatctt
    15781 catttgtaaa ataccattct ctgtcccagt gcgtgtgttg aattatatgt gcatttacca
    15841 tatcagattc tcctactttc cttcttctac tgaatttact tgttgttctt ttttatactt
    15901 gatggatttt agtgcattga ccattaacgc tgtaaccact cccccaataa caattgatac
    15961 taaatataaa ggtatattcg taacaaaagg aattgccata aaggttgaaa gcattgtttc
    16021 ttgagtaaca ccaaaaaatc catttagacc acatgctact gcagtgccca ccacgatgct
    16081 tggtattact cgtaaaggat ctttcacggc aaacgggatc gtcccttcag taatccccac
    16141 aaggcccata atcatagctg tctttgcatt atctcgttcc gattcatcat aaagtttttt
    16201 gtttaaaatt gttgctaaaa acattccaaa tggcggaata gcaattccta ataacatggc
    16261 tgtactaggt ccaaagcttc cgcttgaaaa agaagcaacc ccaaaagcat atgcgatttt
    16321 attgataggt cctcccatat caaacgccat cattcctcct aaaatagccg acatcaaagc
    16381 agcatttgct cctgacatgc cttctagcca attagtcagt cctgttgtca ttgcttgaat
    16441 aggcgtagct agtacatacc ataatatgat tccaacaact aatgtagtaa caaaaggaat
    16501 cagcataatt gattttagac taataacttg catcggtact tttacttttt tcaatgcatt
    16561 agctaaatag ccacaagcta atccagcaac cattcctcca ataaatccca taccaagatt
    16621 ttgacacatc atcccagtga taaaagctgg tgctagaccg ggacggtcag caattgaata
    16681 tgcgatatag gctgaaatca tcggtacgat aaactgtaat cccgtctgac caatcattcg
    16741 caggactccc cagaatgtat ccgtctggtc ccaaacacct gtgccaccaa aaatgactgc
    16801 aatagaagtt agtaaccctg ctgctacgac aagcgggatc atataggaaa ccccactttg
    16861 gaaatgattt agaatttctc ttccttgttt cttgaattcc attagtaact cccccacgat
    16921 atatttttta tttgttaatt aatgcaattg cttcatcaat cgtctcttca ccatctttta
    16981 ctacctctga ggtccctact gttaaaatcg gtttaccgtc aaagcgacct cgttctgaaa
    17041 catttgtatc tactgcaaat atgacaacat ctgcctcgga aacatccgaa attgaaagtt
    17101 tgttttctat ccccatagcc ccctgtgttt ctactttaat atctaaccct ttcttcttcg
    17161 caaacttttc taaattagct tttgccatat aagtatgggc aatccctgcg atacatgctg
    17221 ttactgctac tatttttgtc atatgaaatt cctcctttta aaatgataga gcttttacca
    17281 attctttttt atttctcgaa tcttttattc ttttaacaaa ctcgtcatcc atcaatcttc
    17341 cagataattc tgctaaaata cgtaaatgat tagtaccttt atcagtgtct tttattgcta
    17401 acaaaaagac gagatcaact ggttgatcat ctaatgcgcc ccattcaaca gaccgcttta
    17461 acttggctac agccactgtt gattcaatta ctgctaggct tttgccatga ggaatggcga
    17521 tatttttccc aattcctgtg gtcatatggt tttctctgtc aaatacgtct ttttgaaatt
    17581 gtttgtggtc agacacaaac ccattagtct ctaacaattt cgataatttt tcgactacac
    17641 tttttttatc agatgcattt aaatctatca ctatattatt tatattgatt ttttcactga
    17701 tcatttataa gccttctttc aatatataaa ctaatttttc aaacgacgaa ctcgttttta
    17761 aatttgctaa taactcatga ttcgaagcga tttcattgaa tgtttcataa attttattca
    17821 attcaggttt tactttttca ttaagtgcaa aaaagaaaac agaatgaaca agtgtcccat
    17881 cccaatcaat tccttctgga ttaacgtaga cacagattac tggctttttt ataaaatcta
    17941 cttcgatatg cggcatagca aaatcattca tcgctgttga agatagtttt tctcttttga
    18001 ttgctccctc tagtatccct tctttcgcgt aattttcttt taccaatttt ttggtaatta
    18061 tatttaaaac gttcacttga ttatccttta tttggtgggt aaaaaagcaa aatcgtttat
    18121 ctaataaatt tagaaaagca tcttctgttt gttcatttaa ctgtagatac tgttttactt
    18181 ttctgacatc tgcccctaac atcaaaggac taacaacaat aacaggaaaa tttgtatttt
    18241 caatcggaat cgtgcttata acaagttcat cagtgacttt tctatcttgt agttcattga
    18301 tgcttagtac atcttctatc tcaaccatat ctgcaaaacc gttatttatt ctttgctcta
    18361 ataattttga tgttccatat ccgctactac aaactaatat cactttcgtt ttgtcaggtt
    18421 tgtaacgatc caaaagtgat tgaatatgga gtgttacgta agcgatttca tcttcattaa
    18481 aatgtataca gaatctctct tcgatttcct ctaataaatc tacacttatc ataaatgacc
    18541 ttttaaaaga atatttgatt ttgtccgtat aagggttata gatatttact cccaatttga
    18601 gtcttttaac tgctgcattg agatgtaata ctagattatt gatcaattct tcatcaggat
    18661 aaaattcatt taatttaatt cttactaact cttgtagctc aacacccata tctaaatagg
    18721 aaagcttcgt ttgatctaag tcgtgattca tagcattttt ttcaatcgaa gtaatgtgaa
    18781 tgtcaacata agcttgttca tctattggca caataattga aaatgctttt tcaatcattt
    18841 tgattaaaat aagtgtattc tttgagattt cttcttcaga attattacta cgtgataaga
    18901 aattgcttgt ttcaatcctt tctatcgcaa tagcaaggtg gatagctaaa gtttgaaatt
    18961 cataatctgt aaatttcaat tgtgtcttat tcatgtatcg attaagcaat tcggctactt
    19021 ttttcaatat gtccttattt aagaaattat tcgcttcacc aatgaaattg atattcaagt
    19081 ctaagagatc tgttttactg ttagatgctg taaagccacc ccaataatag ttcacaactt
    19141 ctgacatcaa ccttctttta cttttttcat cagctttgat gcgcacgcca tttcgatctg
    19201 aaactaagga catcccatgc ttgtttagtc tttttctaac atctttcatt gtgctttcta
    19261 ccgtcgacct gctcacgtat aattcgtcag caagattttg aatctttaga gaatcattag
    19321 tattcaataa tttagcaaac acataagtta gacggtcctc cggagagtct gcgatattga
    19381 ttttatgact ggataataaa tttcgaagtg tttctttttt tccaataagc gaaacgcctt
    19441 ttttaggttg aaccaacatt tgtacatctg gcattttttg gattatttct ttcaaaatta
    19501 aaatatcttt gctaattgtt ttcgtagata catataattg ttctgataat tctttgtaag
    19561 ttgtgtgctc attttcaatc aaaaagtata gtattttcat ttgccgttcg ctaaattcga
    19621 tgcccattat cttcacctcc tgaatacagc ttaagtgtac aattattatc tctattttca
    19681 tacagaagaa agttcttgat taatgtagaa gtttctaata ttcttgatta tttcaaatca
    19741 ataaaaaagc ctgcaatatt tatatcacag gcttttttct ctattaaata tatataagat
    19801 aatacatttt tctactagtt ctcactaatc ttgtataatt aaaccctccg caacttgacg
    19861 gagatggaga agatgccccc gtctaatcta gcggtcatct ctcctcccat tccttccacc
    19921 aatttttttg acaggtaaag cccgattccg ctagattccg tatttgtgcg gctggtattt
    19981 tcggtataaa aacgctggaa gactttttca acagaggttt gcgttcctgg tttgatagga
    20041 ttggccactg ttaatcgaat gaatggatcc tcttttgaaa atgtcacctt tgcttgtcca
    20101 tctgcataac gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa catgagcgga
    20161 tctgctaata cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat atttttctca
    20221 gaaaattgtt cataaaaaga gaaaaatact tcttccacct ctttagaaaa agaaagcgta
    20281 tggagatcca tttctttttg cttcgtctct attcgagcaa gatcataaaa ctgatccgtc
    20341 atactgagta atcttgcaac agattgtttg atgatcgtta aatacttctc ccgtttctcc
    20401 gtatcctcta cctgttccag taattgtaca taaccgttaa tagcagtcaa aggagttctg
    20461 agatcatgag aaaggccgac gatcgatagt ctgatgtttt cttccatttc ttgaaatgct
    20521 tgtccttttt gttcattttc atccaaccat gtattcagtg catctactag gtccattaga
    20581 ttcttttctc gaaattcgac tgaaagacga ccaccatatt cttgtcgttc agaaagagtg
    20641 gcgattttct tagttaatct cttgatttcc ctttgcagga agaaaataaa acttcccata
    20701 aagataacaa cgagaagcaa cagccattta atcaaatcta aaccccactc cccacaccgt
    20761 atgaatatac acagcatgtg cctttgctag cttccctcga agattggaaa tatgcatatt
    20821 cactgttttc tcactatcaa aatatggttc ttgccaaact gtttcataaa gatttgctcg
    20881 cgaaaatact tttttcggat gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt
    20941 caaatggaca gccacaccat tcacatttac atccctcgac tctttgtcca gtaagatttc
    21001 cttgtaatag atgcgttgcg acagatcttt tacttggaca gaagtctgat gacgaagttg
    21061 gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt ttcgtcacat agtcatccgc
    21121 tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa gaacgatgat
    21181 cggaacattc gatgtctgtc ggatttctgc taacaatgct tcacctgaaa gccctggaag
    21241 catgagatct agaaggatca aatcgacagg atatgtgctt aacaataatt ttccttccgt
    21301 acctgaataa gcatcaagaa gtttgtatct ctgttcaaga acctcctcca gtaaggaatg
    21361 aacagcttca tcgtcttcga taatcaaaat agtttccagc aggaccacct acttaatgtc
    21421 agatttttgg aaaataaaca ttcctagagc tgatgatacc acgattgtta ctagactgac
    21481 cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg tatacatgag
    21541 ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc
    21601 gggaatgaag gccgttccca tgatataaag aaaagaaaga agcaaactga cactggtact
    21661 tctagtgatg aatgtcaaag ccactggaac agataatgtc cctaatgtca gcagaagcaa
    21721 tacaggaaat acttttagaa cataatttcc atagtttttg attccctcaa cttcaccaaa
    21781 tccgaaagca agtgtgtaag caagcatggc tagtcccgtg atcaaaacca cgccaagtgt
    21841 tgtgatcaaa agcgtaacga gaagtttcga cacataataa gtcattcgat tccgtcctaa
    21901 agataacgta ttgcgaatcg taccgttttg aaattcttcc ccaatataaa agcacgcaaa
    21961 tgctgcgaaa gccagaagaa tccatgcagc cattttttct accattaaaa tccctgttgc
    22021 accgttgatg actgtatccg ttgcttggtt taattcattg ccgtattttg tatgcatata
    22081 ccattcgttc caagaataaa aaagcggaag tacgaccatc agtcctagtg agacccacat
    22141 tttacggctc ttccatactt ttattttttc agcagttaat agattaaaca tgtggattgc
    22201 ctccagttaa ttgtaagaaa taatcttcca gtttttgccc aactaggtga ataccatcca
    22261 caggaacttt tgcttctacc aatgcttgat tgatggctgc tacttggtca attccttcaa
    22321 aaagctggat aacacggcta gattgtacaa aataatccct ataaccaaga gagtcgagaa
    22381 ccgttactgc ttgttccgtc gccgaagtgt caatttttat aaattgacga gattcttgtg
    22441 ccaattcttc tttcgataat tctttgatca agtgtccttc atgcaaaata ccatagtgag
    22501 tggcgatctg tgacaattca tctagcaagt ggctagaaag caagacggtg atgccccgtt
    22561 cttttaccaa acgttggatg atttctcgca tttctgccat ccctgctgga tccatcccat
    22621 tcgtcggttc atccaaaatc aaaaattctg gattcgtgat cagtgcattt gcaatagcta
    22681 aacgctggcg catacctaaa gagaaattct ttgctttctt tttccctgta ttttccaatc
    22741 gcatcatttt cagcaggtct tcgatttctc tatccgacac accgccgttc gctgcttgga
    22801 tacgtaggtt atctcttgca gttaattctg gataaagagc aggggtttca atcgattgac
    22861 ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc gaaccttcat
    22921 caatcgagat caaacccata atagttctca taaaggtaga cttacccgct ccattttccc
    22981 caatcaatcc atagatcatt ccacgtttga tttcgattga tactttatct aatgctttgg
    23041 cagcaccgta ttttttggtt ataccagttg ctttcaagac cgtttcattt ccaaaatcca
    23101 tacttatcct tctttcttat tgatgagtga agtatagttg acgggtataa agaaagcagc
    23161 cagaaatgta taaagaaagt gtaaattttc caattgaatg tcaaaaaaaa caatcggaca
    23221 atacgtgtct ttcctattat caggcagttt gttatacgct tgtatcagtt tcctgattct
    23281 ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac tgaaaatttt tgcttctttc
    23341 agatcaagct tgagtaattt taccagaccg gttataagga taactatagc aagactccct
    23401 cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg tcttccaatc aaagagcgca
    23461 ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat cgttgacata
    23521 agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata aaaagcaaaa
    23581 gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt tgggatttac
    23641 aattagctac caaataagag gttatgacaa aagacttttc acaatccctt cttccgaata
    23701 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa acatgagaag
    23761 caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcagaa
    23821 gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt atagatcgaa
    23881 tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa aaaggcactt
    23941 tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc agcattgctt
    24001 cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta acgttttgag
    24061 aactgtgaag ctttacgggc tttcttaaga cctggttttt tcttcgtatc attgaataag
    24121 tttgaaaaac cttgatttat aaaggttttc ccgtaaatat attttattat atttgattaa
    24181 atttgaaatg tttgtgttct aaaatgtgtt ctaatttaaa ggcagtatat ttcgtatttt
    24241 agattaaa
//
LOCUS       DAYYBG010000035        23755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000035 DAYYBG010000000
VERSION     DAYYBG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.035"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            380..1819
                     /locus_tag="ACHAUN_001364"
     CDS             380..1819
                     /locus_tag="ACHAUN_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8460073.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2114..2332
                     /locus_tag="ACHAUN_001365"
                     /pseudo
     CDS             2114..2332
                     /locus_tag="ACHAUN_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2581..2796
                     /locus_tag="ACHAUN_001366"
     CDS             2581..2796
                     /locus_tag="ACHAUN_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460074.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3013..3269)
                     /locus_tag="ACHAUN_001367"
                     /pseudo
     CDS             complement(3013..3269)
                     /locus_tag="ACHAUN_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3456..4367)
                     /locus_tag="ACHAUN_001368"
     CDS             complement(3456..4367)
                     /locus_tag="ACHAUN_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8460075.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4386..5390)
                     /locus_tag="ACHAUN_001369"
     CDS             complement(4386..5390)
                     /locus_tag="ACHAUN_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8460076.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5387..7510)
                     /locus_tag="ACHAUN_001370"
     CDS             complement(5387..7510)
                     /locus_tag="ACHAUN_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8460077.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7515..9962)
                     /locus_tag="ACHAUN_001371"
     CDS             complement(7515..9962)
                     /locus_tag="ACHAUN_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8460078.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9949..10338)
                     /locus_tag="ACHAUN_001372"
     CDS             complement(9949..10338)
                     /locus_tag="ACHAUN_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8460079.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10398..10901)
                     /locus_tag="ACHAUN_001373"
     CDS             complement(10398..10901)
                     /locus_tag="ACHAUN_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8460080.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10914..11138)
                     /locus_tag="ACHAUN_001374"
     CDS             complement(10914..11138)
                     /locus_tag="ACHAUN_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460081.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11242..13152)
                     /gene="ltrA"
                     /locus_tag="ACHAUN_001375"
     CDS             complement(11242..13152)
                     /gene="ltrA"
                     /locus_tag="ACHAUN_001375"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8460082.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(13898..14035)
                     /locus_tag="ACHAUN_001376"
     CDS             complement(13898..14035)
                     /locus_tag="ACHAUN_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8460083.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(14032..15216)
                     /gene="mobT"
                     /locus_tag="ACHAUN_001377"
     CDS             complement(14032..15216)
                     /gene="mobT"
                     /locus_tag="ACHAUN_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8460084.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(15475..15765)
                     /locus_tag="ACHAUN_001378"
     CDS             complement(15475..15765)
                     /locus_tag="ACHAUN_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460085.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(15755..16897)
                     /gene="dcm"
                     /locus_tag="ACHAUN_001379"
                     /pseudo
     CDS             complement(15755..16897)
                     /gene="dcm"
                     /locus_tag="ACHAUN_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(16973..17137)
                     /locus_tag="ACHAUN_001380"
     CDS             complement(16973..17137)
                     /locus_tag="ACHAUN_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460086.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(17130..17804)
                     /locus_tag="ACHAUN_001381"
     CDS             complement(17130..17804)
                     /locus_tag="ACHAUN_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8460087.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(17882..19222)
                     /locus_tag="ACHAUN_001382"
     CDS             complement(17882..19222)
                     /locus_tag="ACHAUN_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8460088.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(19305..19640)
                     /locus_tag="ACHAUN_001383"
     CDS             complement(19305..19640)
                     /locus_tag="ACHAUN_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8460089.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(19650..20024)
                     /locus_tag="ACHAUN_001384"
     CDS             complement(19650..20024)
                     /locus_tag="ACHAUN_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8460090.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(20037..20351)
                     /locus_tag="ACHAUN_001385"
     CDS             complement(20037..20351)
                     /locus_tag="ACHAUN_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8460091.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(20464..23691)
                     /gene="fss3"
                     /locus_tag="ACHAUN_001386"
     CDS             complement(20464..23691)
                     /gene="fss3"
                     /locus_tag="ACHAUN_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8460092.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         6637 a         5140 c         4099 g         7879 t
ORIGIN      
        1 tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa
       61 taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg
      121 tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg
      181 ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc
      241 tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta
      301 gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca
      361 ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg
      421 ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat
      481 tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga
      541 atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat
      601 tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg
      661 atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca
      721 agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc
      781 catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat
      841 tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg
      901 gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga
      961 tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat
     1021 tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc
     1081 ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa
     1141 agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt
     1201 gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta
     1261 tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc
     1321 tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga
     1381 gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt
     1441 gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa
     1501 tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt
     1561 gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc
     1621 gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa
     1681 ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag
     1741 ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg
     1801 tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg
     1861 tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca
     1921 agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg
     1981 cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta
     2041 ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg
     2101 taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag
     2161 tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg
     2221 gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt
     2281 gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg
     2341 gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca
     2401 agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt
     2461 tgatttaggt tccttttcta attataataa gatgaccact acagtaacct actgaaatat
     2521 ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt
     2581 atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga ttttacttcc
     2641 aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt
     2701 gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga atttgctatg
     2761 aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga attacttgcc
     2821 acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg aatagagcat
     2881 acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc tatccccgta
     2941 gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat ttttgaccct
     3001 tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt taattcggtt
     3061 ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca ttcatctaag
     3121 gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc tgacattaca
     3181 tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc aagaagaaaa
     3241 actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat atagactgat
     3301 tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc gagttaccga
     3361 atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata agttacacta
     3421 cttataactt ctaataaaat tagattgagt cattattatt taacatcaac gattttccag
     3481 tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt tgatacatta
     3541 tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag ataaactgga
     3601 ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc attttcaaca
     3661 taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa
     3721 tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac ttgttttgct
     3781 tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac taaatgattc
     3841 ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg cgtctttttt
     3901 ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga gtgtttttca
     3961 acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata
     4021 tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact cattctcttt
     4081 tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa
     4141 ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc atgagttgta
     4201 tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca agacaataaa
     4261 agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg ttcttttttc
     4321 ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct ttttcctctt
     4381 ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt ttccgttaga
     4441 ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc gtaaatgccc
     4501 acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa atcacctggt
     4561 ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc cacccgagga
     4621 agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc aaaccccgtt
     4681 tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc ctcatcaaaa
     4741 atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt cactaataaa
     4801 cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat cgcgattggg
     4861 ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc agctaaagca
     4921 aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc atagttatag
     4981 gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac caaagacgca
     5041 aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg tggtaatccg
     5101 gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac ttgaataatc
     5161 gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt ttccaccatt
     5221 gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt gccagaacta
     5281 ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat acccataaaa
     5341 gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat tttttctcac
     5401 ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg gtctgctttg
     5461 gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct gattggcttt
     5521 tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga ttcagatttg
     5581 ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga ggttgagctg
     5641 ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct tcactcagta
     5701 tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca
     5761 ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct tttttcgact
     5821 tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt acctgatcct
     5881 ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg ccgatttttc
     5941 tcccagccaa attcattcgg gattgtttct tattatttga aactggattc tccttagtta
     6001 agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg
     6061 atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact gctgcaccac
     6121 caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt cgattcatta
     6181 acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta ctctggaggt
     6241 tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc acaatttgca
     6301 gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc gtggagatgc
     6361 taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta ttgaataaac
     6421 gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat aagaaactaa
     6481 ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata atccctgtta
     6541 gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc acggtcccta
     6601 aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca atctctgcct
     6661 tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat agaatcttgt
     6721 tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt agtaaccaag
     6781 gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta tcttttcctt
     6841 ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct tcactaacat
     6901 ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg aacgaacccg
     6961 atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg gtttctcgtt
     7021 ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat aatagaaacc
     7081 caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat gtttggatat
     7141 tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca
     7201 cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta gtaatggcat
     7261 aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc aaccaatccc
     7321 aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg gaatatagat
     7381 ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac
     7441 tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt
     7501 tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc aaaggcatgg
     7561 aagagttctt caaagactgg atgtacctga accacgccta cccgaccata caaatcctgc
     7621 atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc atcttctcga
     7681 tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact gcgaaacgca
     7741 aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact attttgcgtg
     7801 acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt cgataatgtt
     7861 ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat tttgaagatt
     7921 tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag catggaaaca
     7981 ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg taaaaccaaa
     8041 tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt aatcgaacca
     8101 tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg atccgcgata
     8161 ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa taatccccgt
     8221 tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt ctccccatct
     8281 cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc agaatccttc
     8341 tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt actagttaag
     8401 ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc accccgttcc
     8461 ccttttggat cgacaatcac agcttgtcca ccaaaaagga cggcgtaata aactaagaga
     8521 ttgttactaa aggactttcc gccaccgagt gaaccgagga atgccgctgc tagcgcattg
     8581 gtaactgaat ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac attgcgcccc
     8641 gtatctacgt tataaccaaa gtaaatgcct tccaactccc ctaacatctg agtggcgcca
     8701 aaaccaagtc cggcgagaaa atcagacgta acatactgga tataatcatt catgtatcgc
     8761 ttactagaag gtaagaattc ttcatgtagc cccatcatat cgccaaaagg ccggactagt
     8821 tttacattgg tatcatcata aaaatctagc acttcatcac accggcgctt tagttcatct
     8881 actgattcgg cacttactcg aaccacatag ctcagtttgt acatggattc tttggactga
     8941 tccaaggtag tttccaattc atctacggaa tccaatgcat ctaagacatt ggaatttgtt
     9001 tcattgtcag attgataggc atggttgtcc aagtctttta attccttttt cttattgcga
     9061 acggtagcaa gtgccttttt gttcgtcaca atctccacat tcatactagt gtcaattggg
     9121 aatgtaaatt gttgttgctg gtaatagaaa agttctgagg agggaaactc catttccccc
     9181 acaattgtat taattgttaa gtacgctaca tatgattcat gattctcatg ttccatgcat
     9241 aagtaccggg gcttttcttc aatcaaacaa cgactgggac gtaataagtc gtaacgtttc
     9301 accaaagttt ccgacttcag ctttttcttt ggtagctgaa attcatattc ttcaaatggt
     9361 gtaccctttt cgccatagat atgttcaatc aaatacatca gatcactcgg tgtgactcgt
     9421 ctgactttaa atcttcgagc caatttactt tccattagtt tttcaagctt ggtatatcgg
     9481 cgaatttctt cattggataa ggaaacaaaa tcgcccatca aatgatgatt cactccatag
     9541 acaaattctt gaaagccgga gaagaaagat ttcttgagat tctttagatt gacctcttca
     9601 tctgtcgcaa tcagtttgaa gccaataaaa aagcgataat caatctgact atcgccaatc
     9661 atggatacta atgcctctgt ctgtaaatca attcgttgtt tcgctacttc tgttaatcgc
     9721 ccggtaattt ccttttttga tcgttcttgc gtggcacgaa tactactttc tgtggcaatt
     9781 tgtaaggcat gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa gttatcgtgg
     9841 acttgatatt tctgttcagg tgacaaaaag gagtaattgt aaggtattaa ctcatagtag
     9901 gcaaaacatt ctccctcttg attaaagaca agattatttt caatatactt aatcgggtac
     9961 atagttcaca ctccttacca gagtaatggg ttcgtcccat cgtttcttct gttctttgac
    10021 tttctttcct gcgaaagtga ccttaaatcg taagtgatag ctaataaaag atcgtaaaaa
    10081 tcccaatggt ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa ctgccaccgg
    10141 tatacctaag tattttaaaa aggccccctc tatcatagat agtggaggca catgggcaaa
    10201 tacgatgaca aacagcaagg ataaaacaaa ccaagccatc tggttaaaag tcacaggaaa
    10261 aggcagttga aagtcattaa ttgcataaat gaccttttca actgcccaaa tactggtgta
    10321 gctttttatt ttcttcatca aatcattcct ttaaaaagaa ggatagttta gaaatcaact
    10381 acccttcaat aattttgtta atatggatac tcaaaaacac ctcgactcgt taccaaataa
    10441 ttgcctccaa tctccaagtc tctaccacac gcatcaaaat caatatacgt cttcaagtgt
    10501 tctggtactt ctccaaaatc accagcttct acaatcaaga aatatgctac atcactcata
    10561 tcatcgcaat ctggataata gataatatca tccacatgct ctaccatctc ctcaaaagaa
    10621 ttgaagaaag catgttgaat ctcagaagca acttcaccaa ctggcgttcc ttcaagttcc
    10681 tccgctaagc cacacaaccg attaatttcc tcaattggcg tatactcatc aatctcaaag
    10741 ggaagttcat aatcatgaat tgcatattcc tcgtattcgt catttaaccc aattcgttct
    10801 ttcacttcat caaaatcgac aggtggggta aaccacgccc ccaccaattc accttcgtta
    10861 tatttcccga ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc cccttacgca
    10921 ccgacaattc ggttaaacaa actgagtaag acatccttca ccccagaagc attaaagacc
    10981 aatccgaccg caatcaaagc aatcaccaaa aagccaatca gtttggaaaa ctcccgctta
    11041 aatcctaaaa acaaaccaat gaccacaatt gccagtaaga ctaaggattg ggcattggtt
    11101 aaaaaccatt gatatatgga taggtaagtt cctgatatta taaatatctc aaacttttcc
    11161 ccccattcac acggagcgtg cgactttcac cgcactccgc gttccatcgt gtttatattg
    11221 aaaagtattt atgtagtaga cctatatcaa gtttaattct tgattgccag ctaatactct
    11281 atatttattt agtcttatga gctggacttt atttagcttc aatagagcta tatatttttg
    11341 tattgtttct tctgttttag cgtgtattag acggtgtacc gcctctttta ccaaaataag
    11401 atttttataa tcgtcatttc caccagctcc agttggtaca atatgatgac aatgtatttc
    11461 attagcttgt tgaaattctt ccagagttat agcacatttt ccatgttgag cagaaaatag
    11521 cgaaattcga ttgtctgcgt attctattgt gcgattttcc aaagataatt tcattaactg
    11581 gtacatcagt aatttatcaa cacttagatt ggtgtggata aattttcttc cctctagagt
    11641 gtagcaattc gctaaataca cctttgccat tggttttctg tgttggatat atccaacagg
    11701 gaaaataggt tcgccctcgc cagcaacata ccgaagcatt ttagatttac catatcttgt
    11761 tgcctccttt gtcgtaagtt gtctgcctgt ttttctcaat ctgccccttt tgtttgtacc
    11821 taatctgtta gtgaagatag ccattacagc tctattcagt ttcatacaat ccagatttac
    11881 attcgttgca atctgataat aattttgcat acctaatacc attgaattat aaagtcttgt
    11941 ttcttctaat tccgttttcc cttttgaggg agaagcaatt ttctttgcct gtgataccag
    12001 cttgtctttt tggtttttga attgtttatc acttatatgt gatttaacaa ctaacttctt
    12061 tcttctcggt atcattttaa tcttgaaacc aagaaagtcg gagtatcttt ttctaacatt
    12121 tacaattctt gttttttctt gtgatacttc caattttaat cgttgttcaa tccattgagt
    12181 gattgctatt ttggcttttt cagcactttc cttgtagcgg cagaaaactc taaaatcatc
    12241 tgcataacga accatataca tttcctttaa ctttgttttc ttcatttctt tgtaagcgtt
    12301 gcttttacat gggctaccgc ttttattgat atgaaccaca tagttttttg taacaggatt
    12361 ttcctgccat tggctttcta cccaatggtc taactcatta agaacaatgt ttgctagtag
    12421 tggggatatt attccccctt gcggagttcc tttctctgga tatgttattg taccgttagt
    12481 cattttgatt ggagctttta atatcctttt taaaatgaat atcaagtgct tgtcccgtat
    12541 tcccatagcc catatctgtt ttatcagctt ggaatggttc acgttgtcaa agaaaccctt
    12601 aatatcaaat tcaatcacat aatgtagctg gctgatttgt aggcggttat aggttgcttt
    12661 tattgcattt tcaactgacc tattaggtct aaaaccatag ctgttttcac taaatttagc
    12721 ttcgcagatt ggttctaata cttgttttat acattgttgt ataagcctgt cccacataca
    12781 aggaatacct agaggtctgg ttttaccatt tggttttggt atttcttttc gcctcactgg
    12841 cttaggtcga tacccatgtg gactgcctgc aacaatgtat cttacttttt tgacaactgt
    12901 ttctgctggc aatttgccaa tatctttaat tattgtaccg tctgttcctg ctgttttact
    12961 gcctgcattt gctttgatat ttcgatatgc cagtaatata ttttctctcg ataggataat
    13021 atccataaga tgagtgaaaa tatctccatt cttacttttg gcatataaat catcaaaggt
    13081 gttttgcatt tcgtaatatt cagcgtgtct taaatcttct acacacaatg tttcattttt
    13141 atttttaggc ataaggcttc actcctttac ttgaaattga gtgagctttt ttatcttact
    13201 cgtaatcctt attgaaacaa cataatactt ttctaatata tacacgactt gagggcattc
    13261 ctccattctc attacaagaa tttcttaggt tcgccctctg cttttcagca ttagttcaac
    13321 tgcttattgt tcttcgtcag tttattctct tgatgagaac ccaatacctt tcctcgttcc
    13381 gataatctta tctatacata tatatcctta ggtcactagc tataagcctg ctggcttgat
    13441 accgcctgta acgttatatg gcatttcata acctatattc ttactttgcc acaccagcta
    13501 cgatttctcg tacctttgca tttctacaaa gttcaaattt agacccgtac attcgctgtt
    13561 tcgtcagttc cttatagaac attctaacca tagatatttt atagaccccc gacctgttgg
    13621 atacgctacc cacctctgga tagttttcga tagaagatat atgttcatca actattacgg
    13681 tatctttcag ccgtctttac cgagcttcaa acattctgag tttcctcata caatgcttgt
    13741 cggaggctca gggcaggcgt ttcagagcgt tacttcatca ttccacctgt cagattatca
    13801 gttctcctaa taaccaaact ttttcgttct tttattagtg ccttagcttt tcactaagga
    13861 acgagtcgca cataaatttt gtccgaaatt catcttgtca ctttcctttc ttttccattg
    13921 ctcgatccgc tgctgcactt gcattcatca agcacatcac agtaacacca agcatactgc
    13981 caccaaatac caaaaaaata tcttttacaa ttcccatgac gattcctctt ttcatataat
    14041 caaattttca attgccatac tttgttgctc caaaatcttt ttatgtcggt ctgataagcg
    14101 tgcattctgt accatatcct ggatataggt tgtccgattc aatcgatcca actccttagc
    14161 cattttccac gtaggggcga cttgtcggcc tagccaattc aaggttcgtt caatggtata
    14221 cggttttggc tcggaagtta attgaatctc tttccgattc cgtccaataa aacgccccca
    14281 ttgttcattc aagggccagt tggttcgcct tttaccttct actttatccg caaatcgcaa
    14341 gtaacgattg ataatggaaa acgtggtgct ctcaatgcta cgacctttta acaaatcttg
    14401 aatcgcatga aaagcacggt catttttcag tcgaatctca aaacgatttt tcgtctcagt
    14461 ttggtctaat ggaatcccta acttgatata ttgttcatag tctttttcat acaaacagaa
    14521 ataaacctca ctcttgagac tgccgatata taaggtattg cccatgccgt ccttttcatc
    14581 agcctttaaa agttccccgg aacgataact tttaaaggta cgaaacaagg agatacattc
    14641 ttccttctga cattttttcg ttagctcagg tatatccaat agccccactc gatcattgat
    14701 tgccaagtcc aagcgtttca tcacgccacc ggcctttaga caatcctcga aaaagtcgta
    14761 ccagctacgc ttttgagcga gtaaaaaagt ttcaaattgt cggcaacctc gcccctttaa
    14821 ttctaaaagc acacctttat cttcttcatg agacaacatc accacaatat cacccatgac
    14881 atactgttct tgataggagt aaaaggcata atcttcatgg agcatatact caaatttcaa
    14941 atggagaatt tcttgaataa tttttcgtgc atccgttgtg ggaaagcgaa tccttacata
    15001 gtcaaacagt aacgtcaaag gcaaatcggg attaaagctt tccagttggt caaaaatttt
    15061 cctcatggtt tttatagatg cggttacgcc accattttct atctgattta agtacggccg
    15121 actaagtcca caagccgtag ccaatttatt ttgtgaaacg ccgtattcgt tgcgtcggtc
    15181 ttttaatagc cgataatcta aattccgttg tgccaagttc tcacctccaa attcaaggtg
    15241 ttaacttttt caaatgagtt aacacctata caaattactt actatctttg atacaacaag
    15301 cattcttaat ggttgcttac ttattttttg tgtttttgta cccccctgtt agatacgggg
    15361 gtgagacatc atggtcggct aacgccgtcc atgaacaggc acagcaccac ggaggatttc
    15421 tgcttcgcga atcctccgcg gtcagcgcct aacagtcagt tgtgttggtt taggttactc
    15481 atctgtaata ccatttactt ccaactctgc tttcatttct aacaagtatt gacgaatatc
    15541 tggttctttt gcttggtccg caatccgctc aaattctgca attacttgat ttatttcaat
    15601 tcgagcatcc gtctcctcat tcattaaata tggatcgcca gcgactttga caccattctt
    15661 gcgaatatca aattttaacc gtcgcaattg ttcttctaat tctgggtctg actgaatctt
    15721 tccaatcacc caataaattt ctccaatcaa ctcatcataa accatgtaca tccaactctt
    15781 ttaatcttct ggcaatatca aaaatgaccg gaactgttac agagtttccg gcttgcttat
    15841 acaactgact atctgaattg acttgagaat ctcgatcaaa agcccaatcg ggaaagccct
    15901 gaagtcgcca acactctcta ggtgttagct tccgaatttg atactgatcc gtgacgatcg
    15961 cttgttggtc tcctgttagt aaggtatgaa cactctgttt tcccagacga cctcggcgac
    16021 ttgtggaatt aggaaaagcc agattcacac tgtcttctgg cttcacctct tgatattctg
    16081 tttcattttt tcgaatcatg atttttgtac caccttgttt cgttgacaag gtgggggcgg
    16141 ttccagtaac ttcataaact cgattacctt gttcaaactt tcccggtaat agtcctgcta
    16201 cagagattcc atcccttgct ggcagtgcga ttttgattcc ttcatcacct gttaaggttg
    16261 gcgctacgcc atctgaacca tagacttgtc cacccattcc tcgattggaa ggattgatat
    16321 ttaccaaatt tctgatgttt tgatcagatg ttgaagacgg tccaatgata ggaaatactt
    16381 gttcggtaca tgcgtctcga gaatgtccga taaggaatat ccgctcccgg ctttggggaa
    16441 tgtaggcctt tgagttaagc acctaccact ccacgtcata ccccaatcca tccagcgttc
    16501 ggaggatcgt ctcgaacgta gcccctcctt cgtgattgag caatcctcgg acgttctcaa
    16561 ggaataaaag cttaggtcgg agaagagtgg tgaaccgagc gatttcaaag aataaagtac
    16621 ctcgagtatc ggaaaatctt tgccgctttc ccgcaattga aaaagcttgg cacggaaatc
    16681 cgccgcaaag gatgtccact ggtccgaggg attgaataaa ctcatctgat acacttgtga
    16741 tgtcatgcat ttccacctcc ttacttgtat catgaattgc tttataacta cgtctagcaa
    16801 atttatcaat ttcacaaaaa ccgatacaat gatggctcgc ctgttccata cctaaacgaa
    16861 agccaccaat accggcgaat aaatctaaaa atctcataca ttacctcatt cattgcaaag
    16921 aaaaaagcca agaacatttt gctcttggct catacattat taatttgtcc tactattttt
    16981 tatttaatcc aactaatttc cgttgttttt cattggcctg atcggctaca tatttaatca
    17041 ccgttttgaa ctcttttgca gtagcatttt ccgataaatc cgcaatgctc ttattgtatt
    17101 tctttacaat tttcgttact tgttctttct cagtcatgta attgaacctc caaatctttc
    17161 ctactatacc ctagtggtaa aaataattct agattatccg tagcataatg aacttcggaa
    17221 atattattcg tataatcttt tattatgata tgaagcaata catctggatg tttactcttt
    17281 acttttgcaa tgttctctct tgatttgaaa aaagcgttaa taaatgcctt tttcggcaca
    17341 tgacgtccct ctgcaatttc acgttcttgt gtaaatttcc aagcgatttt agggtcttga
    17401 taaacataga aaatcgtcac tcgaaaatgt tttttcaacg cccgcgagat atttttttca
    17461 gcacttaata ttgcaaaagt tgcatccaga ataaaagaat agccatgatc taagacatat
    17521 ctaaatgcct gctctaccaa ccaagaagat gctttttgaa agagactaga gttttttcca
    17581 ttataatcag gaaactgggt gcgaaagtaa tccgcatcaa taatcacatg gttatcataa
    17641 ttctcagcta atccaatggc aacctctgat tttccagccc caggactccc tgccataaaa
    17701 actgcgtctc gtccaacttc gggggatttc cctttgataa tataagaaat aaacttatcc
    17761 ttatttgctt ttgcatactc taaataagaa tcattacttt ccatattaat tatccccttt
    17821 cttcctacta tataaaagta ttatacgtta cttgaagtat aaagtcagta ttgaagatca
    17881 ctcaatcatt catcttttct aatttactaa ttgaatctaa gaaatcatac ccttttggta
    17941 caagtggcgt ataaaactca ctaatcacac tgccacctgt atccacataa cctcgacctt
    18001 tgatgtgctt cataaagaag tttttttcga cctccccaaa catcatggaa tatccgagtt
    18061 cactcatacg acccaaagcc acgcgaaagt tgaattgatc gcggattccg tctcctaaat
    18121 atttcgcatc cggcctttgg caagccaaaa tcagaaagaa accagattga cgacccaaca
    18181 tcacgatttg tttgagctta ttcaaaatca ccgcactttc tttcgtcgtt aacatctcca
    18241 tataagccac gtattcatca aagattaaaa agttgggtgg aagtccaaga taggcataat
    18301 tctctcctgt tttgtagtta gacatttctt tcattgcctt gctacgagcc atcatacgtt
    18361 catagaaatc ttccacacaa gcagaaattt cttccttttg tgaataaaca tgaggcatca
    18421 ccgtacctaa atcagctaaa tctgcatttt tgggatcgag gataaacaat tctgcatctg
    18481 acttcaatag tgcttcgata atggtgagga ggaaataggt cttccctcca cccgtgccac
    18541 cagcgattaa catatggggt aaagaatcat aagcccatac ttgatttttc atcaatcgca
    18601 gagtcccatt ttctgccact acttcgtcta tcccaattcg attggcgatc atatcataca
    18661 gtaaagtata ctccacatag gagtctttga gttccttttc tactaactca caatacagtc
    18721 cgctttctaa cttcttttcc aattttaaaa gctgatcttg gtatttccca agtgaaattt
    18781 gaacgcgaat agacagtaat ccatctttca ttcgataaca gattttgggg aagtaactga
    18841 tagtctctct ggttcggcta ctattcaaat ctttgaaaaa gccttcactt tgagtttgtt
    18901 tcacttcata ccaatggttc tctagaatca tccgagctag cttttgtcga tgccacaagc
    18961 ttcgccaatg atcaagaaaa aagtgaatat aaagcaatat catagctcct gaaaaagcga
    19021 gacttccaag cagacatact aaatctttta tagaccaaac gatttcaata gtagaaagtg
    19081 atagcttaga aatatcaaat tgtctaaact cttgaaaata aaagacgctc atccaaccga
    19141 tgaacagagc gagtagccag ccaagaacaa agtgataaac caagtgctga tctcctgcgc
    19201 gtatgcgatg tcctttgtac attttgatgg tttccacctc caatcgaaaa agcccaatag
    19261 gatttcatta ggcaatttca aaaagttatg ctagtcattt actgctacga ggaggggagt
    19321 tgtttcgttt caaccaattg atttaaaaag ccttcaatag catccggcat ctccacacca
    19381 atttcttcca aaatggattc tgcctgccga cataagtttt catttacttc gacaactata
    19441 aagaaagact cttcttctcc taaccagtct agtacatcag agtcttcttc actgtcgcta
    19501 ttttctccaa gtaaaccttg atgtataatc agaaacattt tttcatcaga ggttaatgct
    19561 ttaacgagtt gttcctcagt cagctcgcct tttccatatt gatcgagaca atctaaaata
    19621 tgttcaacat taatttccat aatcttcacc tatttctttc tttcattttg gttcgtagga
    19681 tttccttgat tcggctgccg cttcaagact aaatcatccg ccttaatgaa ccaattaacg
    19741 gtagttcctt ctcgaaaaac ataattggca acggtatcca cgattggatt gaccaactct
    19801 acctctgcat cataagcaaa ctcgcgtaag ggtacattag ctgggatact aacttgaatc
    19861 attcgccctt gtgcagagga ttttaaatta taggttcgtt cttttacctc attaccaatt
    19921 gttccatcct cattttgctt ccgtacttct cggcgtaatg cggaaaaacg caacttccca
    19981 aatgttaaac ctgaatctac gacaatacct tctgttaatc tcatcttctt ttcctcctat
    20041 gctttcacta aatcatcaac atacaacacg taatcagtaa acccacgatt ctcaatctca
    20101 tagcctttgg caaccaaatg gggattcacg gcttttagcg gttggtcttg atcaaaatcc
    20161 ttttcaccgg cttccgcagc tactaccacc tcaatatcat cggcccgttg cttactggaa
    20221 tacaaatgat aactgcgacc aatcaccgta gtattccgtc catatccttc tgttataatt
    20281 tctccttcac cggcataact taactttcca aaggtttcag ccatatctgg cacgacaaat
    20341 ttcaattcca tcttcttttc ctcttttcat tgttagtttt ggatacaaaa atagcgcgac
    20401 tactttttta ggaggcggtc gcgcacgttg gttcttagga tttacttgta tcatctcatc
    20461 accttagttc cggcgattcc agaaataata gcccgccgtc gcaaaaatca aagtaaaacc
    20521 aatgaacagt aaaacattgg aatttttctc cccggtttga gggaaggtct tcactgtggg
    20581 actagattct tgagagccac ttggaacagt tggtgtttcc ggttgtttcg gcgtacttag
    20641 tatggttgga acttcttttg gtgttaaggt ttgagatttt tctttcaaat cttcattgtg
    20701 ttttccattg atattcccat ctttatcgta attgatttcc gcaaaagtaa acttggttcc
    20761 ttctggatat tttccggtat ctactttttt cgcaagcaca gttttggtga attctttatc
    20821 atttacttca taatcaatct tgccagattt ccaaatttct ttgtttgtac catccggtaa
    20881 taaagcatac agaattgttt cgaaagcttc ttttgagcca tccagtacat catgagtaat
    20941 cgacacatca tcaaacatat ccaccacatc accatgagta aaggtctgag aaccgttttc
    21001 taggtgggcc tttgtttgga tagaaacatg gtgttccact gtacaactga cagtttgggc
    21061 ttggttattc aagctaacat ccttcgctac tggcttatcg cctgcttcaa aagcttcctt
    21121 attttcatac acatagttga ataagacaat agttttattt aaaacttgtt cggcggttaa
    21181 tttatgctga attttccaag ttcctgtttt ctctttgttc gccaacgttg cgcttgtttc
    21241 tgcaatcacc actggtttgg ctttttcatc tttttcttgg gcagcttttg tcgcatccac
    21301 atcaatggct tgcgctacca cataccagtc ttctttgatt tcatgtagat tgtaactcaa
    21361 tttatcaact agctcggtag aatccagaga agttaattct ttgtttccgt ctttccaagt
    21421 cgcaagagaa gttaaactat cttcttcctc aggcaaatca tagagcatta aacggttcaa
    21481 actaacagaa aatgctttgt tcgtcagctt ctcgtaagga acggtcaccg ttttaattgg
    21541 ttgtttttgg tcttgttcgg taatggtaaa gacatactca ctcttcacaa agtcttcttt
    21601 gttttcctta aaagtagaac ggatttctaa tggcgtaatc ttttgaaaac cttctggggc
    21661 ttctacttct tcaagtaaat aatccccata aggaagattt tcaaacttgc cataaccatc
    21721 aaaccctaat tgctcgttat aagctgtggt cgcttcatct tcggcacctg tgatttcatt
    21781 ggtcccttcc aatggcgaca ctttaaaggt cagatcattg aagccagttt cggtagtgcc
    21841 cgcagctgat ccagcaaatt taaagaaatc aaagccaaag cgaatcactt gttcttttgc
    21901 cgtaacattt cgggtaatca cggcattttt ttcgctatca tccacctttt tgatggatac
    21961 aggatacttt gtttcatcaa ggctatatcc ttcgggcgct tttgtttctt gccaataata
    22021 ctcgttgata gctaaatgtt taacggcaac ttggttcttt tcgtctaaag tcaaagtgac
    22081 cgtttcatca gaacctttcg ttcccttcgc taactctggt ttaaaagcct cactccattt
    22141 aacagctttt ccatccttag cagtaaacaa ggtgtattca gcaccctcaa agactgcctt
    22201 accttgtgct ttatccccag tatctttgtc ttctttcgtc aaagtggttt cgccagtgac
    22261 ttcttgattt tgccctttta cgttactggt aacaggggct acggtttgat tggcatactt
    22321 caattcgact tttacaggtt tgaacgtatt cacgaaccca tgactagctt tagtttcggt
    22381 cacataatag gttcccaatt ccaacgcatt agcaatctct tttggtgttt ccgcatgacc
    22441 gttttcatcc gtggtcattt cttgaacgat ttcaccggtt ggactgtctt tgcgaatggc
    22501 aaacgtatta ccagctaagg aataattgtc attccaaaga tcactccctg tttcaactcc
    22561 gcttttatcc agaatgattt gccctttttc ccgtgcgttt tttgaagtca cgtaaatggt
    22621 ttcgcctgct ttaatggtgg tagtcatggg ggtggtatca atcgtataag gcgccggtac
    22681 tgatttttcg gtaatggtta cttttgtacc atgaggaatc tcatccaatg tagcaatgcc
    22741 ttctttgtca gtagtcacgt cttttgcagg taaagttttt ccaaagtcta aatggaaaac
    22801 cgttcctggt acaacatcac ccgattcttt atcgactttt ttgatcttta aagaaccctc
    22861 cgtttccaca tcaattttga ttgtgtaggt attaggttta tcaatcgccc cggccattaa
    22921 agtctgttgg ccagcctttt tataggcgac aggtgttccg gtaccagcag atttcttaag
    22981 cgtcagcaca ccactttctt ttgaattggc atttggggtg attatcaact gattaccatt
    23041 cacacgataa tcaatgtttg cggcattttc cacaacctca tcgaactctg acaaattcag
    23101 attattcgta tccgtcacag tagtcgattg ccctaatact gttttagccg tactattatg
    23161 gaaggttggt ttcttctgat aatcgtcaat ggcttgattt atttttgcct caatggctgc
    23221 gatatttacg gcactaccat ctgatcgttt tattgagtga agggtatacc cattaacttc
    23281 ttgccagatc atcttctgtg ccacaatatg agtatctgca tctgtaccta cttttttcca
    23341 taaaacggaa actaatttgg ctttatcgga catatctggt aacgggtttt tctcatagcc
    23401 aggagtgaac tcattgtaaa tcgggactcc tggttcaata cagaaaatat cttgtttaaa
    23461 gccgttgtac actgcataat gcggaacagt gacttctgac attggacgac catcactaaa
    23521 agttccttca acagcatatt gatgtgctaa atctagttct acggttaccg tttgtggatg
    23581 ggtaatttca tcagcaaaag taatcgtagg agcaattgct ccaacaattg cttgaacagc
    23641 caaactcaaa atagttaata cacataattt ctttttttgt tttttataca tgaaatgttc
    23701 tcctttcaaa ttaagcacaa aaaaataaga tgctcgcttg aacatctttg tttat
//
LOCUS       DAYYBG010000036        23645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000036 DAYYBG010000000
VERSION     DAYYBG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.036"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHAUN_001387"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHAUN_001387"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8460093.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACHAUN_001388"
     CDS             complement(1174..1869)
                     /locus_tag="ACHAUN_001388"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8460094.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACHAUN_001389"
     CDS             complement(1882..2598)
                     /locus_tag="ACHAUN_001389"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8460095.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACHAUN_001390"
     CDS             2928..3536
                     /locus_tag="ACHAUN_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8460096.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACHAUN_001391"
     CDS             3861..4184
                     /locus_tag="ACHAUN_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8460097.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACHAUN_001392"
     CDS             4366..5736
                     /locus_tag="ACHAUN_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8460098.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACHAUN_001393"
     CDS             complement(5866..7167)
                     /locus_tag="ACHAUN_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8460099.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACHAUN_001394"
     CDS             complement(7521..9512)
                     /locus_tag="ACHAUN_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8460100.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACHAUN_001395"
     CDS             complement(9656..9922)
                     /locus_tag="ACHAUN_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8460101.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHAUN_001396"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHAUN_001396"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8460102.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHAUN_001397"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHAUN_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8460103.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACHAUN_001398"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACHAUN_001398"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8460104.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACHAUN_001399"
     CDS             complement(14082..14603)
                     /locus_tag="ACHAUN_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8460105.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACHAUN_001400"
     CDS             complement(14606..15448)
                     /locus_tag="ACHAUN_001400"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8460106.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACHAUN_001401"
     CDS             complement(15441..16166)
                     /locus_tag="ACHAUN_001401"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8460107.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACHAUN_001402"
     CDS             16342..16638
                     /locus_tag="ACHAUN_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460108.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACHAUN_001403"
     CDS             17206..18798
                     /locus_tag="ACHAUN_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8460109.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACHAUN_001404"
     CDS             18811..19551
                     /locus_tag="ACHAUN_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8460110.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="ACHAUN_001405"
     CDS             19719..21068
                     /locus_tag="ACHAUN_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8460111.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="ACHAUN_001406"
     CDS             complement(21196..21786)
                     /locus_tag="ACHAUN_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8460112.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21933..23285
                     /gene="brnQ"
                     /locus_tag="ACHAUN_001407"
     CDS             21933..23285
                     /gene="brnQ"
                     /locus_tag="ACHAUN_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8460113.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6771 a         4761 c         4503 g         7610 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtatctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caatcaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacaaactt ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcca tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgaacaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaacagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatta ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact cttttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagtt aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccgtcg ttttatatag gaaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact
    21961 atctttttat aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc
    22021 ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg
    22081 ttaccggtat cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag
    22141 gtgtatatga attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc
    22201 tttacttagt tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga
    22261 ttggtttagc accatttatc gatcctagca gacagacgct atatctagca attttcagta
    22321 ttgtattttt tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg
    22381 ttggaaagtt cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt
    22441 ttttgaatcc actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg
    22501 cttttttcac agggtttact caaggatata acacattaga tgcgcttgct gctttagcat
    22561 ttgggatcat cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg
    22621 ctaaagacac gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt
    22681 tgctcgctta tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga
    22741 tcacgctggc tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat
    22801 ttatcgttat attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa
    22861 cattcacaga tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc
    22921 ttccttgttt agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt
    22981 tgatgttcat ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt
    23041 ttaagcagcg tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt
    23101 ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt
    23161 tatatgttgc ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag
    23221 cagttatcgg ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag
    23281 attgataaaa gaggttgtga aagagacgtt cagccttatt gccgataggc tgtctcttga
    23341 acactgttta ttgagaggtc gtgaaaaagc cgctcagttt cgagtattaa gaaatatttt
    23401 ttgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt
    23461 gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa
    23521 gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac
    23581 aacatttctt ttataatata gattttagac atatatatga tataaaaata gttttcgcaa
    23641 aaaaa
//
LOCUS       DAYYBG010000037        23584 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000037 DAYYBG010000000
VERSION     DAYYBG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.037"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..875
                     /gene="purR"
                     /locus_tag="ACHAUN_001408"
     CDS             57..875
                     /gene="purR"
                     /locus_tag="ACHAUN_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8460114.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1378..2751
                     /gene="glmU"
                     /locus_tag="ACHAUN_001409"
     CDS             1378..2751
                     /gene="glmU"
                     /locus_tag="ACHAUN_001409"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8460115.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2845..3816
                     /locus_tag="ACHAUN_001410"
     CDS             2845..3816
                     /locus_tag="ACHAUN_001410"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8460116.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3822..3924
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3990..4559
                     /gene="thiT"
                     /locus_tag="ACHAUN_001411"
     CDS             3990..4559
                     /gene="thiT"
                     /locus_tag="ACHAUN_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8460117.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4640..5413)
                     /locus_tag="ACHAUN_001412"
     CDS             complement(4640..5413)
                     /locus_tag="ACHAUN_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460118.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5680..7380
                     /locus_tag="ACHAUN_001413"
     CDS             5680..7380
                     /locus_tag="ACHAUN_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8460119.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7350..7568
                     /locus_tag="ACHAUN_001414"
     CDS             7350..7568
                     /locus_tag="ACHAUN_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8460120.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7588..8478
                     /locus_tag="ACHAUN_001415"
     CDS             7588..8478
                     /locus_tag="ACHAUN_001415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8460121.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8496..8780
                     /locus_tag="ACHAUN_001416"
     CDS             8496..8780
                     /locus_tag="ACHAUN_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460122.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9267..9881
                     /gene="gmk"
                     /locus_tag="ACHAUN_001417"
     CDS             9267..9881
                     /gene="gmk"
                     /locus_tag="ACHAUN_001417"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8460123.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9886..10191
                     /gene="rpoZ"
                     /locus_tag="ACHAUN_001418"
     CDS             9886..10191
                     /gene="rpoZ"
                     /locus_tag="ACHAUN_001418"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8460124.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10444..12852
                     /gene="priA"
                     /locus_tag="ACHAUN_001419"
     CDS             10444..12852
                     /gene="priA"
                     /locus_tag="ACHAUN_001419"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8460125.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12867..13358
                     /gene="def"
                     /locus_tag="ACHAUN_001420"
     CDS             12867..13358
                     /gene="def"
                     /locus_tag="ACHAUN_001420"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8460126.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13351..14289
                     /gene="fmt"
                     /locus_tag="ACHAUN_001421"
     CDS             13351..14289
                     /gene="fmt"
                     /locus_tag="ACHAUN_001421"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8460127.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14289..15647
                     /gene="rsmB"
                     /locus_tag="ACHAUN_001422"
     CDS             14289..15647
                     /gene="rsmB"
                     /locus_tag="ACHAUN_001422"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8460128.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15660..16400
                     /locus_tag="ACHAUN_001423"
     CDS             15660..16400
                     /locus_tag="ACHAUN_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8460129.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16397..18466
                     /gene="pknB"
                     /locus_tag="ACHAUN_001424"
     CDS             16397..18466
                     /gene="pknB"
                     /locus_tag="ACHAUN_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8460130.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18631..19530
                     /gene="rsgA"
                     /locus_tag="ACHAUN_001425"
     CDS             18631..19530
                     /gene="rsgA"
                     /locus_tag="ACHAUN_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8460131.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19543..20193
                     /gene="rpe"
                     /locus_tag="ACHAUN_001426"
     CDS             19543..20193
                     /gene="rpe"
                     /locus_tag="ACHAUN_001426"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8460132.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20194..20832
                     /locus_tag="ACHAUN_001427"
     CDS             20194..20832
                     /locus_tag="ACHAUN_001427"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8460133.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20987..21841
                     /gene="mreC"
                     /locus_tag="ACHAUN_001428"
     CDS             20987..21841
                     /gene="mreC"
                     /locus_tag="ACHAUN_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8460134.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21845..22354
                     /gene="mreD"
                     /locus_tag="ACHAUN_001429"
     CDS             21845..22354
                     /gene="mreD"
                     /locus_tag="ACHAUN_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8460135.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            22609..>23584
                     /locus_tag="ACHAUN_001430"
     CDS             22609..>23584
                     /locus_tag="ACHAUN_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8460136.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT         7941 a         4248 c         5069 g         6326 t
ORIGIN      
        1 gaagaaaaac ttatataatg taaaacgata aaatttctta aaaggagttg gaataggtga
       61 aagtacgtcg cagtgaacgt ctagttgaca tgacacagta tttattgaat cacccgcatg
      121 aattgatttc cttaacaagc tttgccgaac gttatgaatc tgctaaatct tctatcagtg
      181 aagatttggg aatcatcaag aaaacattta aagaacgagg gtatggaacc ttggaaacaa
      241 tcccgggtgc tgcaggtggt gtattgttca tccctgaaat ttcatatgaa gaggcgaaaa
      301 aatatattga atctctagca gaacgtctat cagaacaaga ccgtctgctt ccaggaggat
      361 atgtttatct ctctgatttg ttaggagaac ctgaactgct gcgtcaagtt ggaagaatca
      421 ttgcttctaa ataccttggt gaaaaaatcg atgctgtaat gactgtagcc acaaaaggcg
      481 tacctattgc ccaagctgtg tcctattatc tgaatgcgcc attcgttatt gtacgccggg
      541 actcaaaaat aacagaagga tcaacagtaa gtgtcaacta tgtgtctgga tcttctgaaa
      601 gaattgaaaa gatggaactg tctaagagaa gtttgaaacg tggttcacgt gtccttgtag
      661 tggatgattt catgaaaggc ggcggaacag tcaacggaat gaagagcttg attgaagaat
      721 tcgaatcaga acttgttggc atcactgtat ttgccgaatc aaaattcaac ggtcatcgcg
      781 ccatcgatga ttatacgtca ctgctttatg tcaaagatgt agatactcaa acgaaaaata
      841 ttacggttgt gccaggaaat tatttttcag aataagaggt tgtgaaaaag ccgcttagtt
      901 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca ttttttcata aatttggctt
      961 aataccgaga aactggcttt tgaacaccgt ttatctagag gttgtgaaat cagcgtcctg
     1021 tcctgagtat tagctcaata aacggaaaaa ttgctcctca tattttgccg tttattgagc
     1081 taataccgaa ggtcagctga ttgaaaaacg tttaatgaag aggttgtaag cctaccgttt
     1141 aactctaagt cacaagaaac gatttcaaaa aacagctttt catgttttat gagtagtgtg
     1201 acttgtgacc gaggagttgg caggagagca ccggttacca aatagatagg cccgttatgg
     1261 tctatctatt tttttatcca gaaaacgctg ggataaaaga aaatcacttg ttttgttgaa
     1321 aaaatgggta tactgggata aactgaaatc agcaaactat atttaggagt gacgcagttg
     1381 gacgcacgtt atgcaattat tttggcggca gggaaaggta cccgcatgaa gtcaaaatta
     1441 tataaagttt tacatcccgt ttctggtaaa cctatggtag aacatatcat taatcgggtc
     1501 agtgaaacga aaccagatga agtgattacg atcgttggac acggagcaga acaagttaaa
     1561 gcacaactag gagaacgcag taaatatgcg ttacaagcag aacaattggg aacaggacat
     1621 gctgttttgc aagctgcttc tttcttggaa ggaaaaaaag gaacaacgtt agttatcagt
     1681 ggcgatacac cattattgac tactgaaacg ctgaacaatc tttttgaata tcaccaagga
     1741 aaaaatgcaa gtgcaacgat cttgacggca caagcagaaa acccaacagg atatggacga
     1801 atcatccgtg accatattgg gatcgtagaa aaaatcgtgg aacaaaaaga tgcaacacca
     1861 gaagaagctc ttgtacaaga aatcaataca ggaacttact gttttgacaa cgaagctttg
     1921 ttcgatgcgt taagcaaagt tggaacaaac aatgctcaag gtgaatacta tctaacagat
     1981 atcatcgaaa tcttaaaaga agaaggccat acagttgctg cttatcaaac ggatgatttt
     2041 gaagaatcaa tgggagtcaa tgatcggatc gcgttagcta aagcaaatga gatcatgaga
     2101 aagcgaatta accagatgca tatggtcaat ggcgtaagct ttgtcgattc agcaacgaca
     2161 tacatcgatg caggcgtaga aatcggtcca gacacattga tcgaagcagg tgtacaaatc
     2221 caaggaaata cagtcattgg ttctgattgt gtcatcgggt cacattcaaa aatcgtagac
     2281 agtaggattg aggatcacgt cgtcattgaa aactcagtca ttgaaagcag ccatgtcaag
     2341 aaacatgcag atgtgggacc ttatgcacat ttacgaccaa aagctgaaat tggcgaaaac
     2401 gtccatatcg gaaactttgt ggaagtgaaa aatgctcaaa tcggcaaagg aactaaagtc
     2461 ggtcacttga cttatgttgg tgatgctacg ctgggagaag aaatcaatgt cggatgtggt
     2521 gttgtcttcg tcaactatga tggaaagaac aaacatcata cgactgtcgg cgaccacagc
     2581 tttattggtt ctagcacgaa tatcatcgga cctgtcgagg tagctaaaaa cagttcgatt
     2641 gctgccgggt caacaattac agacaatatt ccagaatatg cgctggcaat tgcgagagca
     2701 agacaagtca ataaagaagg atatgcaaaa aaactccctt atctaaacta agacttttgc
     2761 aaaatcactt gatttattta aaaaatatga ctactattta ttaggtagaa ttctagagtt
     2821 gtgaaaaaga aagtggaggt ccccatgtca aaacattact ttgacccaag attaaaaatt
     2881 tttgcgttaa attccaatcg cccattagca gagaaaattg cagcagcagt cggcgtcgag
     2941 ttaggaaaat catctgttac tcaatttagt gatggagaaa ttcaagtaaa cattgaagaa
     3001 agtattcgtg gatcgcatgt ctatgttatt caatcaacga gcagcccagt caatgataac
     3061 ttgatggagt tgctgatcat gattgacgca ctgaaacgag caagtgcaaa aacaatcaac
     3121 gtagtcatgc cttattatgg ctatgcgcgt caagaccgta aagcacgtgc acgggaacca
     3181 atcacagcga aattagtagc aaacatgatt caaaaagctg gtgcaacaag aatgctgact
     3241 ttggaccttc atgcagtcca aatccaaggt ttctttgata ttccagtcga tcatttgatg
     3301 ggtgcgccac ttattgccaa ttatttctta gaaaaaggaa tcaaaggcga tgatgttgtc
     3361 gttgtttcac cagaccatgg tggtgtcaca cgggcacgta aattagcgga atttttgaaa
     3421 tctccgattg cgatcataga caaacgccgt ccaaaagcaa atgttgcaga agtaatgaat
     3481 atcattggac aagtcaaagg caaaacttgt gtactgatcg atgatatgat cgatacagca
     3541 ggtacgatca cattggctgc caatgctttg aaagaagctg gtgcaactag tgtgtatgct
     3601 tcatgtacac atccagtatt atccggccct gctttacaaa gaatagaaga ttcagctatc
     3661 gagcgtctag ttgttacaga ctcgatctat cttccagaag atcgtaaaat cgacaaaatc
     3721 gatgaagtaa gtgttggcgg actaatggga gatgcaatca aacgtatcca tgaaaacaaa
     3781 ccagttagtc cattgtttga aacaaaaaat aaataataaa taaactcaca aaggggagtc
     3841 caattgggct gagattgaat ctatttctaa acccttcgta cctgtatcgg ttatgcgagc
     3901 gtaggaattg tgaataaaca gctgtttgac tattttcgaa agctgcttcc tcctttgtga
     3961 cttttttatt caaaggagga agttttttta tgggaaaaaa tagagtttgg gtagaaggaa
     4021 cgattgttgc agcgttagcg atggtgttgt cgttgattcc gatccagatc ggcagcagtt
     4081 tttctatttc attagggcag attccattga ctctttttgc gttaagaaga gggacaaagg
     4141 cagggatgct ggcaggtttc atttggggca tcctacattt tccgttggga caagtttatt
     4201 atttaagtgt cgttcaagtg ttgattgagt acccgattgc ctatactttt gcaggcgcag
     4261 cagggctcat ggcttcaaat gtccaaaaag gctatgtaga cgataatacg aaacaaatta
     4321 gaaaaagcat cgtgtttggt gcattgattg gcgcactttc cagatatttt tggcatttta
     4381 ttgcaggagt ggttttctgg ggagcgtatg cactatgggg gatgaatcct tggctctttt
     4441 ctttcgtaat gaatggtgcc agcgggttga gtacagctat tgtaacgatc atcgttttat
     4501 tagggatcca acaaacgact ccttcgattt ttgtgccaga taaacgacag gagctgtaaa
     4561 taaaaaagaa gcaagaaact tgagacagaa tcatgtccta gtttcttgtt tcctttttat
     4621 tttttgataa gaaatagatc tacttcaatg gcagataaga aagatcggca tcttctacca
     4681 attgccagtg tcgatcggtg acagagtaga ttaaagtagc gatgatttcc tcttctcgat
     4741 accgggtgcc ttttaatttc caaatataat cttcaatcac atagattggg atacgatatt
     4801 taccatcgat caaaccaact tgatcaaaag aaaaaatgat cccttcctgc atcaaattac
     4861 gcatttcttt caataattcc acactgattt tttcaacagg tgtttctttt ttgaaaaaag
     4921 atttttttac attgatctta tctgacaaga tcgaacttaa tttactagca aaaatcctat
     4981 aaaattcatc taaatagcga taataaatcc gattcagctg atcattaaga tcgaagtata
     5041 caaaattatt ctgtagttta taaaaaaacg gcgaatgaag atgtgttttc atatgaccaa
     5101 aatataataa ttctgaaatt tctaaaggtg tcaattcttt cagcatcgtt aaatcagtaa
     5161 agtcgatcca cttcaattca tcgaatccac gcctgttacg agaagaggaa aagaaacgct
     5221 gaatattttc tgtaccacgg attactttca gccctgtatg ggattcatat tctccagcag
     5281 aagcagatgg atcaagcaag agcaagtttt gaggaaaatg cacgatactg tttgcaaagt
     5341 cagtagcagt cagccctttt gttaaaacag cattacttgt ggtatcaata tgtacataaa
     5401 ctgactcagt catcatgttc ctcctcttat ttcctcttaa tctcttttat tttacaacat
     5461 ttgcattaaa aaagcttgtt tcttttattt taaaattcca ttacaaaatc acgttttcat
     5521 tgaaaacact ctcattttca attaattttt gtgatttttg tattttttta tttaaaggag
     5581 gttttatgaa ttgttttctt atggaatcta caaagtcgaa tacgagactc atgacctttt
     5641 tatgacgtac tggtatactt gaagaaaaag gagagcgcga tggagataaa aaaagtttac
     5701 ttttcacaga aaatgtcgct gtattcgcct atccagcaag caaaacaacc ccttccattt
     5761 tttcaaagtc atcaagaaag tagagacgct ttttggcgtt ctgcaaaaaa gcaatgtgct
     5821 tttaaagcag tatcgaaaaa atataccgtc tattttacta ttgctaaaag tagaggagat
     5881 gttattttta ataatctgtt gattgaaggt gaaacttttg aatggaaaaa attttttcgg
     5941 tatatggaag cttgcgctcg tttttttatt aaagaaaact gttgtttttt atttcaatct
     6001 cctctatcag aagaatggtt gcgtattttt cataaaaacg gctatcaagg aaccacacaa
     6061 ttaaataaaa agctggtcta tcatacagca cttgttttag gtggaggagg agcgcacggt
     6121 gcttatcaga ttggtgtgtg gcaggcgctg aaggaacatg atatcaactt tgagattatc
     6181 acaggtacct cggtaggtgc gttgaacggc gcattgattt tacaaggaga tatgaaaaaa
     6241 gctgtgaatt tatggaaaaa gctgtcgaca agacaagtgc ttgcgttacc agaaatggct
     6301 gcagttgaag accttcgtga acgcttctat cgagaaagac gccaaatgac gaaaacagca
     6361 ctaattgaag gcggggtttc ttctgctcca ttggaaaaga tggtcaaaga caatttggat
     6421 ttgtccttac tcaaacataa tcccaaaata cgtttatata cggtgtctac taaactgcca
     6481 gaactagcgg aagtagtgac tgatatccaa cagacaaaaa cagaagaaat accagattgg
     6541 atactcgcct ctgcttcatt ttatcccgct atggcttatc gaaagatcgg aaaaaataaa
     6601 tatgcagacg gtgggtatcg caacaaaata cctattgata ttgcgattaa caaaggagca
     6661 acagaggcat ttgtcgtaga tgtgcaaggg ccaggcccag cgaaaaaaat tcgtatgcca
     6721 gacattttta tccattggaa atgtcaaaca ttgtggacac taggcagctt tctgcttttt
     6781 gatagccaaa ggaaccaatt aaatttacaa ttaggctatc tggaaatgaa aaaacgatta
     6841 ggctattact atgggaactg gtatacattt cattctgtta aacctgcaag aacatactgg
     6901 cgaggatttt tgtcttactt aacaaaagaa attcagttag aattatcttt tttcaaaagc
     6961 ataaaatttt ggcaaaaact gcgtaattta tataagaaca gggtcgtacc agagacatgc
     7021 ggattggcga tgctagaact tttagcaaaa aaacattttt tactgcccaa tgaaatctat
     7081 caagtagaca tgatgattca aatgatctgc caacaagata taaaaagtat gccggatgat
     7141 ttattggcac aaattggtca attaagtact gaagaatggc gaaggtatcg aaaataccag
     7201 caaaaaatac aaaatgagcg cacgaaaact gtttattttg attatctgct acaaggaaaa
     7261 cgcaaggatc gtttgcagca tgaattgctt aagcaaccag ttgacacatt actgatccta
     7321 tatttatatt atctcaagga ggaacaacca tggcacaaga attttcatat gaaatcgtag
     7381 aagaaatcgc tatattgtca gaaaacaaca aggggtggcg taaagaactg aacctagtca
     7441 gctggaatgg tcgcccgcca aaattcgatc ttcgagattg ggcgccagac catgaaaaaa
     7501 tggggaaagg cctgacattg acgaacgaag agtttgaaca actgcaaaaa gcaatagaaa
     7561 atatgtaatg aggaaggacc ctgtcgaatg aaaagtatga ctggttttgg caaagccatc
     7621 cgcgaaacaa aagcgtatca gttagatatt gaaataaaaa gtgtcaacca gcgttttcta
     7681 gatgttcaaa tacgaagtcc taaaatcctg aactttgttg aaaacgattt tcgtcagctt
     7741 attaaacagc agttaagccg cggtcgaatc gaagtatttg tgaatctagc ttatattggc
     7801 aaaaacgaaa aacagatcgt catcaactgg gaattgattg attcacttat cactcaattg
     7861 acagacggaa ttcaacaacg atacggtagt caaaatcaag tgtcaattag tcggatggcg
     7921 gaacgcttcg tgctggacga aaacttcgta acggtagaag aaaaaccgga agagagcatg
     7981 gaagaattgg cacagttagc tctagataca ttgaaaagcg cacttgattc tattgaggcg
     8041 agtcgagaaa aagaaggtca agctttagct ttagtgttga aaaagaatac tgaagaattc
     8101 aaagaagtac taaaaaaact cgatagtttt gtagaaatct atgaaaagga atttcaaata
     8161 aggtatcaaa aaaagctgga ggaatttctt ggagcgaaag tcgatgagca acggttgttg
     8221 acggagctag ccatcttgtt ggaacgcgga gatattcacg aggaactgga tcgtctggcg
     8281 atccatgtac aaaaactaga tgaacttatc gaagcacaat gtcctgtagg tcgggaatta
     8341 gatttcctga tccaagaaat gaatcgggaa atcaacacga tcggttccaa atcaaatgca
     8401 atcgaaatta aaaatcaagt cgtccagtct aagacgatct tagaaaaaat cagagaacaa
     8461 gtacaaaata ttgaatgacg aaagaggtag agataatgaa atattatgac gtaacgttcc
     8521 acgaattaag tggaaaaact gtggttaaac gggatatccc ttctgaaaaa aacggctttg
     8581 acgtatggaa agatgcctgt gcagactata atgagaacga actgtttatt ttgatcaacg
     8641 acggtgctta cgtgacgatg aatcggaaat ttattgttcg aattgacaca gaagaggtag
     8701 aagatccgac agaaaaagca cgttctcgaa aagatgagat catgggtgta gtgaatactt
     8761 tgtccaatat gggcttttag caaggatgct gaacaaagaa atacaataaa taaacagaat
     8821 tggatttctt cgcttagctt ctttgctcta ggaatacatt taaaggttgt gacaaaattc
     8881 gtgtcacaat ctttttttat tttgaataag tgatgttcaa aaaccatttt ctcggtatta
     8941 agccaaattt atgcaaaaat gtgagaaact attttcacaa aatttttctt catactcgaa
     9001 actaatcggc ttcttcacga cctcttatac acggcgttca aaagcttgca tctgcgataa
     9061 taaccccaac caaacgaaaa acatgagaag ctgtttatgt ttggctgttc ttattacttg
     9121 atgcaggaca gcttttttac gacctcttgg tacagggtag cttttttacc aatttctagc
     9181 tataattaaa gataaccttg aaagttgcag aaaaaaagtg caaaataaac gataagtaca
     9241 aaaagactag ttgaaaggac gtcgtcatgt cagagcgtgg attattaatc gtgttgtctg
     9301 gtccatccgg tgtaggaaaa ggcacagtac gaaaagcaat ttttgagagt gaggataatg
     9361 attttcagta ttcgatctca atgactaccc gtaaaatgcg tgaaggggag gtcgaaggcg
     9421 ttgattattt cttccgaagc aaagaagagt ttgaatcaat gatcgaagct ggagagatgc
     9481 tggaatacgc agaatatgtc gggaattatt atggaactcc tttgtcatat gtcaacaaga
     9541 cattggatga aggaaaagat gttttcttgg agattgaagt ccaaggagca aaacaagtca
     9601 aagaaaaagt gcccgatggt gtattcatct ttttgactcc gccagattta gcggaattaa
     9661 aatcacggat cgtaggccga ggaacagatg cagatgaagt aatcgaaaaa cgaatgaaag
     9721 ttgcaaaaga agaaatcgaa atgatggctc tttacgacta tgctgttgta aatgatgaag
     9781 tgccgctagc agttgaacga atcaaaaaca ttattgccag tgaacatttt cgagtagatc
     9841 gcgtaatcgg taaatatatt aaaatgttaa aggagatgta gtcttatgat gttaaaacct
     9901 tcaattgact cactattaga tcgtgtaaat tcaaaatatt ctctagtgat tttagcaagt
     9961 aaacgcgcgc atgaattgga tgcaagtgca cagcctactc tagattcttt tgattcagta
    10021 aaaagcgtcg gtcaagcttt agaagaaatc gatgcaggaa atgtagtcaa tgatccacat
    10081 cctgaattaa aacgtgaacg tttgaagatg gaagaagagg aacgtcaagc acaaaaagag
    10141 cgtgaacaac acgaattgga atcacgtatc cgtgaagagc aaaaaatgta ataaagaaaa
    10201 atagagcgaa catctaaaaa gaaaagcaaa cccagctgtt tccttttcaa acattcgcca
    10261 cttattgtct ggtagaaaag gctgtgtctg aactttttga cacagttctt ttttttctta
    10321 tctgctgtta attttttgtt aggatttggc agaagataag acaaatccat cgatctctag
    10381 taaaataggc aaggaacttt ttgtcgcttg ataagaaaag acaaaaaaag gaggaaacga
    10441 accatgacat taacagctga agtcattgtg gacgtaccta ctatgcagac cgatcagcca
    10501 ttcacttatt tggttccctc tgaaatggaa acagctttgc aagtcgggat gcgagtagaa
    10561 gtcccttttg gtaatggcaa tcgtcatgtc caaggatttg ttatggctat ccaaaaatca
    10621 gaagaatcag ctaatccatc tttgaaggca gttattcgtt tgctagacct tgctccggtg
    10681 gtcaatgaag aattactttc attagctgat tatatgaaaa aaattactta cgcattcaaa
    10741 atcacttgtc tacaaacgat gcttccgagt gtgatgaaag cagaatatga taaattgatc
    10801 tatcctttgg cagacacacc agaagttcag gtattgtttg atcagcgaga agtgatttcc
    10861 tggaaggaag cagaagaaga aggcagcctt tctcaattga ttcgctggcg ccaagagcaa
    10921 ttagtagata tcaaatatga agtccataca cgaaataaag taaaaacgat tcgtttggtc
    10981 cgttctttgt taactgaaaa gcaaatcgaa gaagaatggg caaaattacg gcaaaatgcc
    11041 aagaaacaaa aagaacttct tttgtgttta tctgaactga gccaagaaga accaattgct
    11101 tatttcaaaa ataaaggaat cagtactgct gtcttgaatc aaggaaaaga aaagggctgg
    11161 ctggagttta tagaatcaga acgttatcgc gacccttata aagaccgagt atttgaccaa
    11221 acgacggcat tggaactgaa tgcagaacaa aaaaatgctg tcgaacaaat catcacagca
    11281 ggtcagcagc aaaaagatca ggtctttttg ctggagggaa tcactggcag cgggaaaaca
    11341 gaagtatatc ttcaggcaat cgctgatgtt ttgtcagaaa ataaaacggc tattatgtta
    11401 gtgcctgaaa tcgcattgac tccgcaaatg gtcgagcgat tcaaaggacg cttcggagaa
    11461 tcagttgcgg tcttacatag cggactttcc caaggtgaaa aatatgatga atggcgcaag
    11521 attgagcggg aagaagcgca agtagtagta ggcgcacgat ctgctatttt tgccccgctg
    11581 aaaaatatcg ggctgatcat tatcgatgaa gaacatgaat caagttacaa acaagatgag
    11641 acaccgcgtt atcatgcgag agatctagca atttggcgca gtaaatatca tcattgccca
    11701 atagttctag gaagcgcgac gccctcttta gaatcaagag cacgagctca aaaaggcgtt
    11761 tatcagttat tgcagttaaa ccaccgagcc aaagcagctg cacagcttcc agctattgag
    11821 atcgtagata tgcgtgaaga gtttcagaat catcggacct ctactttttc tgctaatttg
    11881 caggaaaaaa tacaaaatcg attagacaaa aaagaacaaa ctgtcctact gctcaatcgt
    11941 cgaggatatt cgtcttttgt gatgtgtagg gactgtggat ttgttcttcc atgtcctaat
    12001 tgcgatatct ctcttacact gcatatggat acacgttcaa tgcgctgcca ttattgcgga
    12061 catgaagaac ctattcccaa tcgctgtcct aattgtggcg gcaataaaat ccgctactat
    12121 ggaacaggta ctcaaaaagt agaagaggaa ctccgagaac tttatccgca agctaggatt
    12181 ttgcgtatgg atgtagatac cactcgaaga aaaggggcac acgaacagat tttacaaaaa
    12241 ttcggtgcaa aagaagcaga tatattatta gggacacaaa tgattgctaa aggtttggac
    12301 tttccagaag tgacattagt tggggtgttg aatgctgata cctccctgaa tttaccggat
    12361 tttcgttcta gtgagcatac attccaattg cttacccaag tttcaggacg agcagggcga
    12421 gcggaaaaag caggcgaagt cgtgatccaa acattcaatc ctcaacatta cgccatagaa
    12481 ttggctaaaa aacaaaatta tgagcagttt tatcagcagg aaatgcatgt acggcatcgt
    12541 ggaggctatc ctccttatta ctttactgtg aaaataaccg ccagccatcc tgaagaacaa
    12601 gtagcggcaa agaaaatctt tcagattgcc aatcagctaa aagaagtgtt aagccctcaa
    12661 agcttgctat tagggccaac gcccagcatg attttgagag tgaaaaaccg atattattat
    12721 caactaatca tcaaatataa acatgaaccg aatcttcctc atgttttgga tgaaattttg
    12781 aatggaagcc agaaagaaca gcgacaaggt ctttttgtgg caattgacaa cgaaccatta
    12841 aactttattt aagaaagggc caaattatgc gctatcctat attgatccac cctaatgata
    12901 aattaaaaag aactgctcag ccaatcgatg taatcacgga cgagacaatc gctttattag
    12961 acaatttata tgagacaatg attgcgaatg acggaatagg gatcgctgct cctcaagtag
    13021 gacaaaacaa gagaattgct gtgatcgaag tagatgaagg cgagaaattt gaactgatta
    13081 acccagaaat catcgaagca aaaggtgaaa gccttgacgt agaaggttgt ctcagtattc
    13141 cgcatgtcta tggaacggtc aagcgagcag atgaagtgac cgtgcgttat tatgatcgag
    13201 acggagaaga gatagaagtg acggcttttg gctatttagc ccgtgccttc cagcatgaaa
    13261 tcgatcactt agacggtatc ttgttcattg agaaaatgat acagcagatc ccggaagaag
    13321 aacttgaaga atatatggag gaacatcttg atgactaagc tagtatttat gggcacacct
    13381 gctttttctg tgccgattct tgaagggctg atagaatcag gttacgagat ccaggcagtg
    13441 gtaacacagc ccgaccgtcc agttgggcgg aaacgagtaa ttacgccgac accggtaaaa
    13501 aaagcggctc tgaagcacgg tatccgtgtg ctgcagccag agaaaatctc cggttcgcca
    13561 gaaatggaag aaatcattga attggtgcct gatgtgatcg tcactgcggc atttggccaa
    13621 ttcttgcctg aaaagttatt gcaagtacca aaattagggg caatcaatgt ccatgcttct
    13681 ttacttccta aatatcgggg aggtgcgcct gttcattatg ccatcatgaa tggggagaaa
    13741 gaaactggtg taacgatcat ggaaatgatc aaaaaaatgg atgcaggcgg aatctatgcg
    13801 caagaaagca taccaatcac taaacaagat gatgtaggga cgatgtttga aaagcttagc
    13861 cttttaggaa gaaagctatt gctagaaaca ttgcccaata ttttggatgg acaaaaacca
    13921 gtacctcaag acgagtcaga agcaactttt tcgccaaaca tcacacggga acaagaagca
    13981 atcgattggc ataaaactgc agaagaaatt gacaaccaag tacgcggaat gcgtccttgg
    14041 ccaattgcat tcacgaccta tgagggcata cgctggaaat tattatcggt tgaagtattg
    14101 gcagaaacta cagacaaagc acctggtacg atcatccgga aagacaaaaa aaatctatgg
    14161 attgcttgcg gtaaacagac cgtcttagcc atcaatgaat tacagccagc tggtaaagga
    14221 aaacaggccg tacatgattt ccttaacgga agcggtcagc aagttcagat tggtcagcag
    14281 gtgaaataat gaaaaaaatc cctaaaaaaa tgaaaaaatc agtacgatat atggcattac
    14341 tgacattaga gcgagtgaac aagggtggcg cctactcgaa tcttttgttg aatgagatga
    14401 tcaaacaaag cgagctaaat caaaaagatg tgggtttatt tacagaattg atctatggta
    14461 cgattagcca tcagcgacta ttggaatttt acgttgcacc attgatcgct aaagcaaaaa
    14521 aagtggatga ctgggtcaag atgcttcttt atctttctgt ataccaatta gaatttttag
    14581 ataaagtccc tgcacatgct attttaaacg aggcggtcga aatcgcaaaa gtaaaaggaa
    14641 atcctgggac agggaaattt gtaaatggtg tcttgaggaa ttatcagcgt caaggagcac
    14701 cagatctgca aggcattgct gatcctatcg aaagactttc tgtcaaaatc agcctgcctc
    14761 tatggatgac gaaacgactg gtggatcaaa tcggatacga agaaactgaa aagctcggtt
    14821 tatcgctata tcagcctagc catgctagtg cccgagtaga tacacgccga ctttccagag
    14881 atcaggccat cactgttttg caagaagaag agatcacagc tgaaaaaagc caagtcagtc
    14941 catacggtat agtagcgaaa aaagggcatt tagcttctag ctcattgttc cacgatggcg
    15001 tcatgacgat ccaagatgaa agttcgatgc ttgtagcgcc gtctatgcaa atcgagcctg
    15061 aacatcatgt cttagatgcc tgtgcagcac ccggaggtaa gacgacgcat attgcttctt
    15121 ttttagatcc gtcatgcggt ggagaagtgg tcgctttaga tgtacacgaa cacaaagtca
    15181 aactcattga agataatgca gcacgtctag gtgtctcttc tgctgtctac gctcaaaaga
    15241 tggatgctag agaagtcagt aaacagttcg aagcagagca gtttgatcgg attttagtag
    15301 acgcgccttg ttcaggtctt gggctgcttc ggcgaaaacc agatattcgt tataaaaaac
    15361 agccggatga attagcgaat ttaccaaaaa tccaattgga aattttagaa agctgcgcac
    15421 caactttaaa gtcttccggt attctgactt atagcacttg tacgatctta aaagaagaaa
    15481 accaagacgt tgtttctgct ttcttggaaa gacaccctga ttttgagagg attgatgttt
    15541 tgaccaatcc tatgctacaa tcatcaatcg aagagaaaat gttgactttg tatccgcatc
    15601 agttttacac agatggattt ttcatttgtt gtttgcgcaa aaagtaatga ggtgaaaaaa
    15661 tgcagattga atatcaatca gatgtaggaa gacgacgtaa cacaaatcaa gactatgcca
    15721 gtgtatttac cagccgttca ggtctcaagc ttgctgtatt agcagacggg atgggtggtc
    15781 atcgtgccgg agacattgcc agtcagatgg cggtaacgaa tctaggcgaa gcttgggaaa
    15841 aacaagaagc tgttgacgat gaaaaaatcg cccaatggtt cattcaatcg attcaagaag
    15901 aaaatatgct gatttatcag cgtggacaag aacagccgga atataacggt atgggaacaa
    15961 caatcgtagc ggccgctctg tcagatgact ggttcacgat tgccaatgtt ggggatagca
    16021 gagcttattt gattcgtgaa gataaaatca tgcaattgac ggaggatcat tctttagtca
    16081 atgaactagt caaatcagga gaaatcagcg aagaaatggc agtgaaccat ccaaggaaaa
    16141 atattttaac acgttctgtc ggcatgcctg gaacagtcga agtagatgtt tctactcatc
    16201 tttggcaact aaaagatcga ctgctgttat gttcagatgg cttgacgaac atgctgtctg
    16261 aagaagaaat aggaacgatc atcaatcaag aaggcccatt gtctcataaa gtaactgagc
    16321 tgattgatcg tgccaatgaa gcaggcggag cggataatat caccgttctt cttatcgaat
    16381 ataaggagga cagtgtatga tcgagttggg caaaaaatta aatggccgtt atcaaatcac
    16441 tggaaatatc ggtagcggtg gtatggcaaa tgtatttttg gcgcacgatt taatcttgga
    16501 tcgtgatgta gcagttaaag tcttacgatt tgactttcaa aatgaccaga ctgcgattcg
    16561 acgatttcag agagaagctt tagcagcaac agaactcgtt catccaaaca ttgttagtgt
    16621 ttacgatgtt ggtgaagaag acaacatgca gtatctggtg atggaatacg tcaaaggaat
    16681 ggacttgaaa cgatatatcc aaacacatta tcctgttcct tatgaaacag cagtaaatat
    16741 tatgcagcag atcctttctg ctatttcttt agctcataac catcaaatca tccaccgaga
    16801 tttgaaaccc caaaacgtac tgatcgataa tgaaggtgtt gtaaaaatca cggatttcgg
    16861 gattgctatc gctttatctg aaacatcgat cacgcagacg aatacgatgc taggatctgt
    16921 tcattatctg tcccctgagc aggccagagg aagtatggca acgaagcagt cagatattta
    16981 tgctttaggg atcatccttt atgagatgtt gacgggtagc gtaccgtttg atggtgaatc
    17041 tgctgtaaca attgctttaa aacattttca agatgatctt ccatctataa aagcacttga
    17101 tccaaatgtg ccacaagcac tggaaaacgt gattttacgg gcaacggcta aagaacctgc
    17161 agaccgttat aagtcagccg aggaaatgtc tgatgatctt tctacagtat tatcaccagc
    17221 tcgtgcaaac gaagaaaaat ggcagcctca tgtgatggat aatgaaacaa aagttatcac
    17281 accccttacg gaagatgcac caatgcctga ttcattcaaa tctatgcctc tgccaaaaga
    17341 caagccagaa aacgaaacct taccagagga ggaagaagtt ctgcctgatc ctaaaaagaa
    17401 aggacagaaa aaatggtggc tgatcctatt agctattctt gctgttttag gaatcggaac
    17461 agctgtattc tttgcttctg gcgggcgagg agaagttgtt gttcctgatg tttctgatct
    17521 ttctgaagct tctgctagag aaacactcgc aaaagcgaaa ttagaagtag caacaaaaac
    17581 agaagaaatt gcagatgaca aaatcgaaga aggtaatgta gtgaagacag acccggcagc
    17641 gggaacgaca gtcaagcaaa aacgcgaagt cactctgtat attagtagcg gcacgaaaaa
    17701 aatcaaatta gatgattaca gtggtaaaag ttatgaagat gccgaagcaa gtttgaaaga
    17761 attagggttt tctgctgact tgattcaaaa agaagaggaa ttcagttcag aggtagaaag
    17821 cggaatgatc atttcgcaat ctccatcaga agatacagaa gtcgatccta aaacagacac
    17881 gattactttc acagtcagca aaggaccaga aacagtgact ttgagtagtc atgtaggaga
    17941 aaactacgaa actgttcgta attggcttgt tacacaagga ttcaatatca ataatattag
    18001 tgctacttat gactatagcg acactgtacc atcaggttcg atcatcagtc aggatccagc
    18061 ctcgggtgaa gttgtagcag aagaaactta catcaatttt gtcgtaagcc aaggaacaga
    18121 gcctaaattg aatgatatct ccggctatac aaaaagcgaa gcacaaagtt atttagctag
    18181 tgtcggtgca gaatatatcg ggcatgagac ttccgaatat tcaaacacgg tggataaaga
    18241 taaagtgatt cgtacagtac caggggcagg aacgacaatt accaaaggaa tggttgtgaa
    18301 cgtaatttac tctaaaggac cagatccagc gtcttctagt tctgaaaaag actcttcaga
    18361 cagttctagc agctcaagta gctccagtag cacaagcaac tcttcaaaag aagaatcttc
    18421 cagctccaca agttcatcag ctagcacctc agaaagttca aaataagtgc tgaataaaat
    18481 atatcagaag ggaatgcgac attgatttgt tgcattctct tttttgtgtt tctatctctt
    18541 ttgatcaatt ttcaagccat atactttgag aagtttgcat ggaataacac ctactttcct
    18601 cttcgtatgg taaaataaac aaaaacggag gtggcactta tcaaaggaca aatcagaaaa
    18661 gcaatcagcg gtttttacta tgtatacgca aacggagaga cctatcaaac aagaggaaga
    18721 ggaaatttcc ggaatcggaa aatcacgccg cttgtcggag atcaggtgat ctttgagagt
    18781 gaaaatcaaa cagacggcta tcttctggaa atcttgccga gaaaaaatca attagttcgc
    18841 ccaccagtca caaatgttga tcttggaatc attgtcacaa gtatcgtaga gcctgcattt
    18901 tcttataatc tacttgaccg ctttctagtt accttggaat acgagtcgat cgaaccgatt
    18961 atttttttga caaagaccga cttgtcagac gacccagaaa aaatcgaaga gatcaagcaa
    19021 acctatacta aaataggcta tccggtgatt gtacccaatc atacgggtga tacgacagaa
    19081 ctgatccgtt acttcccaga acggctatcg gtctttatgg gtcagtctgg agcaggaaaa
    19141 tcgacgctac tgaatcaaat atcaccggaa ctgaatttag caacaggaga gatttctgat
    19201 gcacttggaa gaggacggca tacaacacgt catgtggaac tgattcctat ctatggcggg
    19261 ttagtagcag atacaccagg atttagctcg attgactttt tgacaattga agcaggagaa
    19321 ctgccgagac aatttcctga atttgtagaa gcttctgtcc attgcaaatt tagagagtgc
    19381 atgcatgcta aagaacctgg ctgcgaagta aaacatagag tagaaacagg tggtatcgca
    19441 cagacacgtt acgacaatta tttgcaattc ttacaagaaa tcgaaaaaag aaaaccaatg
    19501 tatgcaaaaa aagaaggtaa acgaaaatag gaggaaacaa gaatgaagat agcaccatcc
    19561 attttgagtg cagattttgc caatttacaa agagacattg agttagtgga aaaaggaggc
    19621 gcagattaca tccacgtaga tgtaatggat ggccgatttg tcccaaatat cacattaggg
    19681 ccaaatattg ttcaggcaat ccgtcctgtt actaaattac cgttagacgt ccatctgatg
    19741 atcgttgatc cagaaaacta cattccagca tttgcaaaag ctggagcaga tatcattact
    19801 gtccacgtcg aagcaactcc tcatattcat cgggcacttc aaatgacaaa aagcttagga
    19861 gttaaagctg gagtagttat caatccagga acaccagtct ccatgatcaa acatgttttg
    19921 cctttggctg atcaagtact agtcatgact gtcaatcctg ggttcggcgg acaaagcttt
    19981 attgaagaaa cagtagaaaa aattacggaa ctcgctgatc tacgtagaga gaacaattgg
    20041 acttatgcaa tcgaagtaga tggcggaatc gttccagaaa cagcaaaaat ctgtaaagat
    20101 gcaggggcgg atgtcttcgt tgcaggatca tatatctatg gagccgaaaa tccagtcgga
    20161 caaatcgaac gattaaaaga ggcattagac taaatgaaaa ttttattagc tgctggaggt
    20221 aatccgcaaa actggccgca gtctctgctg tccaaatacg atgcctatgt cgggatagac
    20281 cgtggtgcgc tgtacctgct tcaagcaggc tacccagtag atctggcagt gggtgatttt
    20341 gattcattgt cgaacgaaga gcagaaggaa gtatttgcaa aagccaaaga gtatacccgt
    20401 tcccctgcag aaaaagacga tacagataca cagttaggta tcttaaaaac atttgaacgt
    20461 tacccgcaag cggaagtcac tttgattgga gctacgggcg gaagattgga tcatctttta
    20521 gccaatctct ggttaggtat ggaacctcgc ttcaaaccct ttgcccatca gcttattatc
    20581 agagattgtc aaaattattt gtcttattat ttgcctggag agcatatagt aagacagata
    20641 ccagatatga agtatttagc ttattgctgc ttgactcccg tctcgaatct tacattaaaa
    20701 ggcagcaagt atgtattgga aaataaagaa gtagaatatc cgacatctta tgcaagtaac
    20761 gaatttcttt cagataccgc agaattttct ttttcagatg gtatgattgc agttattcaa
    20821 agcaaagatt gatccataaa caaataaaaa aatttgtctt tcaggctaaa aatgacagaa
    20881 tctattattt gctaaaaaaa agcttaagag gagcaactta ccttttgttt cacggcattt
    20941 agtgataaaa ttgaacacaa gagtctaatt aattcgaggt gtctttgtga agaagttcaa
    21001 tcctaataaa aatattatta ttaccttggt tattgttatc atcgtggtga cgatactaag
    21061 tgtgaccgct gcgcagcgag cgaatgaagg aaagcccaat ttcttccaat ccatcgttaa
    21121 tgacagtgtt gcaatggttg ataaaacaat ctctgcccca gtacggtggg tacgaaatgg
    21181 agtagattct gtccataatc ttttttcaac ttatgccgaa aatgagcggc taaaagaaaa
    21241 aatcgatagc tatgatgacg tttcacagca aaacaagaat tacgagagag aaatcgaagc
    21301 gttgaaaaaa gaacttgagt tgaatcagac gctgactagt tacgaaaaag taacggcaaa
    21361 tgtcattact cgttcgcctg acagttggca ggacatgctg atcgtagaca agggttctaa
    21421 agacggtatc gaagcaaata tggcagtcat gtcgcaaaaa ggattgattg gccgagtgat
    21481 cgaggtcaat acggcttcgt ctaaaatcga attactgtca tcctctaatg aaagctccaa
    21541 tcattttcca gtacgggtat cttcggctaa tggcgaagcg tttggtttgc ttaaaaacta
    21601 tgatgaaaag ctccatgcct tagtggtgac ccaattaact ggtgatacgg atatcaaaga
    21661 aggggatgtt gtccagacat ccggtcttgg agggaattct ccagctaact tgccgatcgg
    21721 tacggttatt aaaacgaaac cagatagtta tgggctggat cgggaagttt atgtgaaacc
    21781 ttatgcagaa atgtatgacg tgtcagttgt gacgattgtc caacggctag cggaggatta
    21841 gtccatgttg aagaaagaaa caatgaagta ctatctgcca atcgttttgt tctttttgat
    21901 gttgatagat ggtcatttaa caagaatgct aggggagtgg tcgaaaggca cctatatgtc
    21961 aaatgcccac tttctgatat tggcattatt atgttgcagt atggcgtttg aaaaacgtta
    22021 tttactgatt accacgattg ttctcggggc tatctatgac gcttactata ttggcgttat
    22081 cggtatctat gcagtagctc tccctttaat tgtatggttg atgtatgtaa tgaaagacgt
    22141 tatccatgtc aacatcttta ctgaattttt cagtatgatc atctttgtca cgggttatga
    22201 attgtttacg atggtggtcc agttgatttt taaattagca gtagtaaata acacgtattt
    22261 tattacaagg tttttaggac ctacactgct gttgaacatg attatatttg tattattcat
    22321 ttttcccttt aagaaattat tcagtgatga ataatttctt tttttatttt taggtcgaaa
    22381 gcgaaaaaga atcgttgtta tcttaagaaa tgctagactt aacgcggctt tttcatgatc
    22441 attttctgaa acgaacagtt ttgtcatttt taaaagttgt tttgaaacat ttttgtaaca
    22501 taactattat atcgttgaca cactgctatg gtacaatttt tatatcgtta agaaagactt
    22561 aaatatattt agatatttat taaattaagc cagtcggagg aatgaagagt gaaaaagagt
    22621 ttaatatcag cagtaatggt atgttcaatg accttgactg ccgtagcatc accgatcgct
    22681 gccgcggcgg acgattttga ttctcagata caacagcaag atcaaaagat tgcagacttg
    22741 aaaaatcaac aagcagatgc acaatcacaa atcgatgcct tagaaagcca agtttctgaa
    22801 atcaacacac aagcacaaga cctattagca aaacaagata cattgcgtca agaatctgca
    22861 caattagtaa aagacattgc agatttacaa gaacgtattg aaaaacgtga agatacaatc
    22921 caaaaacaag cacgcgaagc acaagtaagc aacacaagct caaactacat cgatgctgta
    22981 ttgaatgctg attcattagc agatgctatt ggacgcgttc aagcaatgac aacgatggta
    23041 aaagcaaaca acgacttgat ggaacaacaa aaacaagata aaaaagctgt agaagacaaa
    23101 aaagctgaaa acgatgctaa actaaaagaa ttagctgaaa accaagctgc tttggaatct
    23161 caaaaaggcg atcttttatc taaacaagct gatttgaacg tactgaaaac ttcattagct
    23221 gctgaacaag caactgctga agacaagaaa gctgacttga accgtcaaaa agcagaagct
    23281 gaagcagagc aagctcgtat tcgtgaacaa caacgtctag cagaacaagc tcgtcaacaa
    23341 gctgcacaag aaaaagctga aaaagaagca cgcgaacaag cagaggctga agcacaagct
    23401 actcaagctt catctactgc acaaagctca gcaacagaag aatcatcagc tacacaatct
    23461 tcaatgactg aagaatcatc atcagctaca caatcttcag caacagaaga atcaacaaca
    23521 cctgaatctt caacagaaga atcaacagcg cctgaatctt cagcaacaga agaatcaaca
    23581 acag
//
LOCUS       DAYYBG010000038        23455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000038 DAYYBG010000000
VERSION     DAYYBG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.038"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1685
                     /locus_tag="ACHAUN_001431"
     CDS             24..1685
                     /locus_tag="ACHAUN_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8460137.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1755..2102)
                     /locus_tag="ACHAUN_001432"
     CDS             complement(1755..2102)
                     /locus_tag="ACHAUN_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8460138.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2186..3127
                     /gene="opp3b"
                     /locus_tag="ACHAUN_001433"
     CDS             2186..3127
                     /gene="opp3b"
                     /locus_tag="ACHAUN_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8460139.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3128..4171
                     /locus_tag="ACHAUN_001434"
     CDS             3128..4171
                     /locus_tag="ACHAUN_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8460140.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4177..5247
                     /locus_tag="ACHAUN_001435"
     CDS             4177..5247
                     /locus_tag="ACHAUN_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460141.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5237..6190
                     /locus_tag="ACHAUN_001436"
     CDS             5237..6190
                     /locus_tag="ACHAUN_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460142.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6202..7212
                     /locus_tag="ACHAUN_001437"
     CDS             6202..7212
                     /locus_tag="ACHAUN_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8460143.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7497..8318)
                     /locus_tag="ACHAUN_001438"
     CDS             complement(7497..8318)
                     /locus_tag="ACHAUN_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8460144.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8415..8693)
                     /locus_tag="ACHAUN_001439"
     CDS             complement(8415..8693)
                     /locus_tag="ACHAUN_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460145.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8961..10970
                     /gene="metG"
                     /locus_tag="ACHAUN_001440"
     CDS             8961..10970
                     /gene="metG"
                     /locus_tag="ACHAUN_001440"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8460146.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11449..12036)
                     /locus_tag="ACHAUN_001441"
     CDS             complement(11449..12036)
                     /locus_tag="ACHAUN_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460147.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12211..13533
                     /locus_tag="ACHAUN_001442"
     CDS             12211..13533
                     /locus_tag="ACHAUN_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8460148.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13688..14464
                     /locus_tag="ACHAUN_001443"
     CDS             13688..14464
                     /locus_tag="ACHAUN_001443"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8460149.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14457..15026
                     /gene="rnmV"
                     /locus_tag="ACHAUN_001444"
     CDS             14457..15026
                     /gene="rnmV"
                     /locus_tag="ACHAUN_001444"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8460150.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15019..15903
                     /gene="rsmA"
                     /locus_tag="ACHAUN_001445"
     CDS             15019..15903
                     /gene="rsmA"
                     /locus_tag="ACHAUN_001445"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8460151.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16354..16983
                     /locus_tag="ACHAUN_001446"
     CDS             16354..16983
                     /locus_tag="ACHAUN_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460152.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17107..18216)
                     /locus_tag="ACHAUN_001447"
     CDS             complement(17107..18216)
                     /locus_tag="ACHAUN_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460153.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18425..18847
                     /gene="mgsA"
                     /locus_tag="ACHAUN_001448"
     CDS             18425..18847
                     /gene="mgsA"
                     /locus_tag="ACHAUN_001448"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8460154.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19034..19132
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19170..19706
                     /locus_tag="ACHAUN_001449"
     CDS             19170..19706
                     /locus_tag="ACHAUN_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8460155.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19746..21509)
                     /locus_tag="ACHAUN_001450"
     CDS             complement(19746..21509)
                     /locus_tag="ACHAUN_001450"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460156.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21512..23227)
                     /locus_tag="ACHAUN_001451"
     CDS             complement(21512..23227)
                     /locus_tag="ACHAUN_001451"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460157.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7552 a         4297 c         4774 g         6832 t
ORIGIN      
        1 attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat
       61 gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa
      121 aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta
      181 cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg
      241 aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg
      301 cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt
      361 caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc
      421 cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta
      481 cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag
      541 aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt
      601 tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata
      661 aagctgttta taatggacca ttcacattag aagattttga tggaccagga acagatacag
      721 aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa
      781 tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag
      841 cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata
      901 cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac
      961 cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag
     1021 taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg
     1081 taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg
     1141 acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt
     1201 tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc
     1261 aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct
     1321 ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg
     1381 ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc
     1441 gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg
     1501 ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg
     1561 atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca
     1621 aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag
     1681 aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc
     1741 gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt
     1801 agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag
     1861 gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa
     1921 tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat
     1981 gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt
     2041 tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc
     2101 atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga
     2161 agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt
     2221 ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt
     2281 gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct
     2341 aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa
     2401 tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt
     2461 gttagctgga agaatcggac catccttgca gttagggtta caggcaatta tctttggaac
     2521 agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac
     2581 atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt
     2641 attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt
     2701 tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg
     2761 gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc
     2821 aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc
     2881 attgatgact ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga
     2941 aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga
     3001 ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt
     3061 gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg
     3121 aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga
     3181 atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt
     3241 gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat
     3301 ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga
     3361 tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat
     3421 cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc
     3481 aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt
     3541 aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact
     3601 agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga
     3661 taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat
     3721 tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac
     3781 aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga
     3841 atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat
     3901 tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat
     3961 tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt
     4021 aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat
     4081 tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg
     4141 tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa
     4201 gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat
     4261 tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca
     4321 gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa
     4381 atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt
     4441 ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc
     4501 attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga
     4561 caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc
     4621 aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca
     4681 ctggtatgtt atccggaaat actgattgct gatgaaccta caacagcgct tgatgtgact
     4741 attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt
     4801 atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg
     4861 tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat
     4921 ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg
     4981 tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat
     5041 ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta
     5101 tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg
     5161 ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt
     5221 caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt
     5281 caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga
     5341 aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac
     5401 gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac
     5461 gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca
     5521 agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat
     5581 tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt
     5641 gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg
     5701 gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg
     5761 tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca
     5821 agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt
     5881 taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac
     5941 aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat
     6001 tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc
     6061 agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac
     6121 tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt
     6181 tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa
     6241 gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg
     6301 ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc
     6361 gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat
     6421 gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca
     6481 aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg
     6541 atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat
     6601 cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa
     6661 gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa
     6721 tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta
     6781 actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat
     6841 gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag
     6901 aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat
     6961 cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt
     7021 ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa
     7081 ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca
     7141 gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt
     7201 atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa
     7261 aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg
     7321 acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt
     7381 ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa
     7441 gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat
     7501 tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt
     7561 tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt
     7621 ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct
     7681 ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat
     7741 gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca
     7801 gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt gttactatga
     7861 cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct
     7921 ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta
     7981 ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt
     8041 gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat
     8101 gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct
     8161 tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct
     8221 ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa
     8281 tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta
     8341 aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa
     8401 atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa
     8461 agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata
     8521 acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa
     8581 tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt
     8641 gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt
     8701 ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa
     8761 gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc
     8821 ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa
     8881 aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc
     8941 gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct
     9001 attatccaag cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa
     9061 ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac
     9121 atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag
     9181 ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat
     9241 tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat
     9301 tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag
     9361 aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg
     9421 gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc
     9481 gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc
     9541 agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc
     9601 ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg
     9661 tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga tatggctccg
     9721 aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa
     9781 tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac
     9841 ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa
     9901 aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt
     9961 attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg
    10021 tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg
    10081 ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat
    10141 ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg
    10201 aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca
    10261 aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat
    10321 tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc
    10381 caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa
    10441 tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag
    10501 gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa
    10561 tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac
    10621 ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag
    10681 tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac
    10741 tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg
    10801 atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga
    10861 tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac
    10921 aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag
    10981 ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt
    11041 aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa
    11101 taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa
    11161 taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga
    11221 ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta
    11281 ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt
    11341 tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc
    11401 atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt
    11461 atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa
    11521 tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt
    11581 tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg
    11641 attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata
    11701 gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa
    11761 aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat
    11821 cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag
    11881 caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa
    11941 ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata
    12001 atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa
    12061 ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc
    12121 actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat
    12181 accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat
    12241 ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa
    12301 actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg
    12361 ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg
    12421 gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta
    12481 ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc
    12541 tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca
    12601 gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct
    12661 gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga
    12721 ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt
    12781 acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg
    12841 ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca
    12901 attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg
    12961 aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac
    13021 gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac
    13081 ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa
    13141 gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat
    13201 gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac
    13261 tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt
    13321 gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat
    13381 ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg
    13441 acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat
    13501 tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt
    13561 acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat
    13621 tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga
    13681 gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat
    13741 tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt
    13801 cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa atatatacag
    13861 tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt
    13921 acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca
    13981 ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc
    14041 aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca
    14101 aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga
    14161 ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt
    14221 cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt
    14281 acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga
    14341 accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga
    14401 agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg
    14461 ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc
    14521 gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc
    14581 tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt
    14641 tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt
    14701 ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa
    14761 gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg
    14821 ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag
    14881 caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac
    14941 aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga
    15001 aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa
    15061 gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact
    15121 gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg
    15181 atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa
    15241 gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc
    15301 tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt
    15361 agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt
    15421 acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc
    15481 atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt
    15541 tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa
    15601 acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat
    15661 ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt
    15721 cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag
    15781 acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg
    15841 gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag
    15901 taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac
    15961 agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca
    16021 agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa
    16081 ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg
    16141 caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc
    16201 ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg
    16261 atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat
    16321 ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta
    16381 acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc
    16441 atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt
    16501 tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca
    16561 acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct
    16621 tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat
    16681 tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta
    16741 gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat
    16801 ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat
    16861 aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg
    16921 gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat
    16981 tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga
    17041 tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg
    17101 ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct
    17161 gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt
    17221 tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc
    17281 ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc
    17341 actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc
    17401 aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat
    17461 aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc
    17521 aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa
    17581 tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt
    17641 aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc
    17701 tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc
    17761 ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg
    17821 ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt
    17881 gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt
    17941 catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc
    18001 cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc
    18061 cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa
    18121 ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata
    18181 ttttttatat acgtttttca gagccatttc taccataata cgttccacac tttctttttt
    18241 agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga
    18301 acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata
    18361 agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc
    18421 tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc
    18481 aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca
    18541 gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg
    18601 ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg
    18661 agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga
    18721 tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact
    18781 ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc
    18841 attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa
    18901 aagctaaaca aactgataat atgcgatata atacgaacgt attggataat ataaacaata
    18961 atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag
    19021 atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg
    19081 cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga
    19141 tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga
    19201 ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg
    19261 agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt
    19321 ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct
    19381 tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct
    19441 acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta
    19501 gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg
    19561 tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac
    19621 cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa
    19681 gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct
    19741 tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga
    19801 agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg
    19861 tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc
    19921 attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct
    19981 tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg
    20041 cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc
    20101 aaacgttcta caaattcata catataagag gcttttaggg catcattgat ttctgcgtca
    20161 cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta
    20221 tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca
    20281 tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca
    20341 agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca
    20401 gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca
    20461 aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc
    20521 ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc
    20581 agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc
    20641 gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg
    20701 ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc
    20761 aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca
    20821 aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt
    20881 cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt
    20941 tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg
    21001 accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc
    21061 actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca
    21121 tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat
    21181 cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga
    21241 ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat
    21301 agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg
    21361 atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt
    21421 atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc
    21481 gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt
    21541 cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac
    21601 cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa
    21661 taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat
    21721 aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc
    21781 ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt
    21841 ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag
    21901 cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag
    21961 tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca
    22021 gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg acatcataga
    22081 aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa
    22141 ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag
    22201 tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag
    22261 tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc
    22321 ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa
    22381 gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat
    22441 ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg
    22501 tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat
    22561 ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac
    22621 gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt
    22681 tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca
    22741 ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc
    22801 cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa
    22861 gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact
    22921 cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc
    22981 cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag
    23041 acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc
    23101 cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa
    23161 atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact
    23221 gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac
    23281 attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt
    23341 attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa
    23401 aacatgccag tctcttcatt cataggaacg tctattttac ttaatttttc tataa
//
LOCUS       DAYYBG010000039        23263 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000039 DAYYBG010000000
VERSION     DAYYBG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.039"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            338..502
                     /locus_tag="ACHAUN_001452"
     CDS             338..502
                     /locus_tag="ACHAUN_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460158.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            499..729
                     /locus_tag="ACHAUN_001453"
     CDS             499..729
                     /locus_tag="ACHAUN_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460159.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            914..1459
                     /locus_tag="ACHAUN_001454"
     CDS             914..1459
                     /locus_tag="ACHAUN_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8460160.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1556..1777
                     /locus_tag="ACHAUN_001455"
     CDS             1556..1777
                     /locus_tag="ACHAUN_001455"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8460161.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1934..2098)
                     /locus_tag="ACHAUN_001456"
     CDS             complement(1934..2098)
                     /locus_tag="ACHAUN_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM8460162.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2200..2721
                     /locus_tag="ACHAUN_001457"
     CDS             2200..2721
                     /locus_tag="ACHAUN_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8460163.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2708..3148
                     /locus_tag="ACHAUN_001458"
                     /pseudo
     CDS             2708..3148
                     /locus_tag="ACHAUN_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3188..3346)
                     /locus_tag="ACHAUN_001459"
     CDS             complement(3188..3346)
                     /locus_tag="ACHAUN_001459"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM8460164.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3439..4200
                     /locus_tag="ACHAUN_001460"
     CDS             3439..4200
                     /locus_tag="ACHAUN_001460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8460165.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4387..5739
                     /locus_tag="ACHAUN_001461"
                     /pseudo
     CDS             4387..5739
                     /locus_tag="ACHAUN_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5755..6975
                     /locus_tag="ACHAUN_001462"
     CDS             5755..6975
                     /locus_tag="ACHAUN_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8460166.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            7010..7687
                     /locus_tag="ACHAUN_001463"
     CDS             7010..7687
                     /locus_tag="ACHAUN_001463"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8460167.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7684..8877
                     /locus_tag="ACHAUN_001464"
     CDS             7684..8877
                     /locus_tag="ACHAUN_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8460168.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8892..9218
                     /locus_tag="ACHAUN_001465"
     CDS             8892..9218
                     /locus_tag="ACHAUN_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8460169.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9222..9557
                     /locus_tag="ACHAUN_001466"
     CDS             9222..9557
                     /locus_tag="ACHAUN_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8460170.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9554..9949
                     /locus_tag="ACHAUN_001467"
     CDS             9554..9949
                     /locus_tag="ACHAUN_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8460171.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9946..10317
                     /locus_tag="ACHAUN_001468"
     CDS             9946..10317
                     /locus_tag="ACHAUN_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460172.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10333..10893
                     /locus_tag="ACHAUN_001469"
     CDS             10333..10893
                     /locus_tag="ACHAUN_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8460173.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            11018..11329
                     /locus_tag="ACHAUN_001470"
     CDS             11018..11329
                     /locus_tag="ACHAUN_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460174.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11353..11544
                     /locus_tag="ACHAUN_001471"
     CDS             11353..11544
                     /locus_tag="ACHAUN_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460175.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11544..15392
                     /locus_tag="ACHAUN_001472"
     CDS             11544..15392
                     /locus_tag="ACHAUN_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8460176.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            15393..16265
                     /locus_tag="ACHAUN_001473"
     CDS             15393..16265
                     /locus_tag="ACHAUN_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8460177.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16262..17311
                     /locus_tag="ACHAUN_001474"
     CDS             16262..17311
                     /locus_tag="ACHAUN_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460178.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17315..18139
                     /locus_tag="ACHAUN_001475"
     CDS             17315..18139
                     /locus_tag="ACHAUN_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460179.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18157..20217
                     /locus_tag="ACHAUN_001476"
     CDS             18157..20217
                     /locus_tag="ACHAUN_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8460180.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20234..20383
                     /locus_tag="ACHAUN_001477"
     CDS             20234..20383
                     /locus_tag="ACHAUN_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460181.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            20380..20826
                     /locus_tag="ACHAUN_001478"
     CDS             20380..20826
                     /locus_tag="ACHAUN_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460182.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20828..20965
                     /locus_tag="ACHAUN_001479"
     CDS             20828..20965
                     /locus_tag="ACHAUN_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8460183.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            21000..21242
                     /locus_tag="ACHAUN_001480"
     CDS             21000..21242
                     /locus_tag="ACHAUN_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFM8460184.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21258..21455
                     /locus_tag="ACHAUN_001481"
     CDS             21258..21455
                     /locus_tag="ACHAUN_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8460185.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21466..22482
                     /locus_tag="ACHAUN_001482"
     CDS             21466..22482
                     /locus_tag="ACHAUN_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8460186.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            22597..>23263
                     /locus_tag="ACHAUN_001483"
     CDS             22597..>23263
                     /locus_tag="ACHAUN_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460187.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYG"
BASE COUNT         8095 a         3934 c         5143 g         6091 t
ORIGIN      
        1 attccattaa taatttcaat ttttgcgctt tgtcttaatg tctatatgat tggatttaaa
       61 aatgggcaaa ataaaagata gtagtaacta agaaagaaat ttttatattg tcactgtggc
      121 ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacta tgcaatgttc
      181 gattcattgc cagtgacttt ggcaaccgag gcatcggcgg tttaaaaata taggggtgcg
      241 caatttcgta cgcgttttgt gcatcgtgta ggttgctatt acatattaga tcactcgttg
      301 agtggtcttt ttattttaca taaaggaggt aacaacgatg tatagaccac aatacttaga
      361 acagaagtat gaagtaatca ctgtgcaaaa cggtaacggt gagatagtac gaaagtatag
      421 aagaccaata aagagcgata catataaacg aaaggaaaac aatgaagtta tttcttttcg
      481 cagaaggaga aaagccaaat gagaaactac tggtatgtat cgctaactaa tgaatatcct
      541 cgaaccattg atgattgttc agtgcgtgtt gtgcgttctg tacaaatcaa agggaagtac
      601 tctattgtcg aaatgctaag agaagctaca ccaaacgaag tggataaatg caagctgata
      661 tattgcggtc atggctattg gaaagacgag tatatccaac aaaacattga gaggtggata
      721 gatagatgag ttacctcgaa catttgaaac gttgctacat gcattctaag aataaacttc
      781 ctgacagcta cactgtagat gatgttgcta tacatgtatt gaagactgag agccatagca
      841 gtcctgatgg tagcagtaag gagcagacgc tcgcgtggtt caagttcttt aagtggatta
      901 aggaggaaga gtaatgcaca tgcacatagg agaaaagata tataaattga gacggcttaa
      961 taacgaaaca atgaacgact tagttaaaca aactggagtg tcaaaatcat ggttatctga
     1021 tattgagtct ggaaaaaagc aacgcgttga ttttaataag atatgtgcaa tagccaaaca
     1081 ttataaagtt agcttagaat actttcggga tgattatttg gatagtgatg aacacgagga
     1141 tattattcct ttgacaggcg aaacaagagt taaatcagtg aaatcgcttg aacaaagcct
     1201 aaagtatctg aataacaaac aagaacctaa ccttctagag atcaaactaa aagatactga
     1261 ctcagtacca gaggtttggt acaagggtga gaggttggat gaattgccta aaggattagt
     1321 agatgtctcg tatcattgga agactgatga ttttactaat gatgatagag gagcgaacga
     1381 catcacgatt caatactttt ctagctttaa tgataagtat ccagataaga aaacaatcgg
     1441 acacaagaga gatatgtaaa tgaaagaagt tagacctaga gacgaaatag acaaactata
     1501 caagaccaaa cgatggcgag acctaaggca agtagtaata gctagggact tcggcatgtg
     1561 ccaagagtgc aagcgtcgag ggcggaacac aaggggcacg atcatccatc acatagtcga
     1621 ggcgagggaa gacctgtcac tgttctggtc cgtagataac cttgaatgta tctgtgtagc
     1681 ttgtcacaac agagagcatc cagagagatc aggcgggaag aagaaaccaa aacctaaatc
     1741 acatatcgtt aaaatgtatt caactcctga aagataagtt tgcagcgaaa tgaaggtagc
     1801 ccccctactc taaaagatta aagagtaagg cttgagaaga acggtgctgt ccttccttcg
     1861 taaaaagacc gcttttcaag ttttttggag aaaaaggaaa aagccgacca atttaagccg
     1921 gctttggacg aagctatttc ttagtccatt tgtttccttt ttgagaagta gggggtaatc
     1981 ggtcgcctgg atcaattttt acttcttgtc cgccttggac atttccacca cgaggaccca
     2041 cttctttgta ggttcctttt ggtttgttgt cttcgccggg tttatagagt tctcccatag
     2101 gaatccctcc ttaacaaatt tcggcacagc actgccgata acctaattat aaggattgtg
     2161 ataacgattt caatctatct tttgaaagaa ggtgatatta tgccgcaacc agcgaagagt
     2221 gcaaaattac aattattaaa cggaaaccca aataagaaga ataccgaaga actccgcaag
     2281 cgagcggccg cagaagacaa attaaaaatg gctactgaca aaatcaaacc gccgttatgg
     2341 ctagattcgc taggaaagga tacctttgag tttatcgccg atgaattgct gtctgtggat
     2401 ttaatcagta atccggacgt ccatacaatg gctctatact ccaattggta ttcgcaatac
     2461 gtttctttag aaaaacagct tcgaaaacta caacgagagt acaagttgaa ctatgcgctt
     2521 gcgaaaaagg aggcagaggc gagaggtgag ccgtttaatg aacctaatga attaattggt
     2581 aatccgctct ctcggcagat ggatacagcg tctcggaatc tccgttcttt tggcgctgat
     2641 ttaggactat caccagcagc cagagctaag ttagctatta agatggctga tgatggtggt
     2701 gatgacgatg acgacttcta atattttgga tatgtcttac acagaacgtg tggactattg
     2761 gcaaagctat cttgaggagc aagcttcttg gggtggcttt ttaaaatgtc catatccgga
     2821 attgttaact acttggtatg cggaacgatt aatcgatgga agcataccag ccagcaaaga
     2881 aaatattcaa gctgctaaac ggcatatgcg tgatttgcag cgccaaggaa cagatgattt
     2941 tccttggatc tttgacgaag aaaaaggtca ccggcctatt agatatatcg aaaaaaaatg
     3001 taaaccaact gaaggcgact ttggttcgtt tgttttgcaa ccttggcagc atttcataat
     3061 tggatccatg tacggatggg tacatcgtga tacaggagag cgtcgcttcc gcgaggctct
     3121 tatttttgtt ggacgtaaaa acggtaagtt ttgccgttta aaaatcgggc aaaatcggtg
     3181 aaaaccttta tttttggctt tcttttagta ataattcgat agctttatca agcaatttag
     3241 acataggaac catagtttct tccgacattt tttttaatcc ttcgtataaa tctttatcaa
     3301 ttgcgttaga aattctaatt ctgtttttta gtccgtattt gtccatgtgt gacactcctt
     3361 tttattttac tataacatcc gattccaccg cttgcaacta ccatcaattg atggtagtat
     3421 ataaaagagg tgagtagatt gaaaaaatac attatttaca aacacttaaa taaaataaat
     3481 ggcaaaattt atatcggtgt cacaaatgat attggcagaa ggtggaggag cggtgggatt
     3541 gaatataaac caccaaagaa cgaaacccaa caccacagaa gtttttggaa tgcaatacaa
     3601 aaatacgggt gggaaaattt tgatcatttg attattgaag aagacttaac gatgaaagaa
     3661 gcttttgaga aagaaaagtt ttatattgaa ctttatgact caactaataa gaaaaaaggt
     3721 tataacatcg caaaaggcgg aaatggcgga ataatatata aagttcaccc caaaggaatg
     3781 ctagggaaaa aacaatctaa agaattttct tcaaatcaca gtaaatgggc aaagaaccat
     3841 aagaataatt gtatgacaaa cggtgatgtt gtatggggtg ttactcacga acacccaaaa
     3901 ggcatgttgg gaaagcacca cagcaaagaa tcaattatga aaaagaaagc atatagtggt
     3961 gaaaatgctg taacctcaaa accaattgtc gcaattgaac gggatggaac taaaagagaa
     4021 tttcacagcg ctaagctttg catggcttac tattcgatta gtacatgtgt tttttaccga
     4081 cttttaaaag atggcgcccc ttatgtgata aatcctaaag cgaactacag aaataaagaa
     4141 aaaatattag ctatcgaagg aattatgttc aaacataaag aagataccga ggtaagttag
     4201 tagattgcga aaggttacta actaccgtaa cgcgtaggag ttgaataaat ataatgctcc
     4261 caagagtgtc cgacagcaga taatttgctg aaaatgtacg ctaaactggg ccagaactga
     4321 ctgaccgatg aaaatgaggg tgacctccag agtgcgagat aaaaagctcg cagataataa
     4381 caatcgaaaa catcactaat ttcaggcctt tccacataca tggtcgctta tgatgatgaa
     4441 caaggcgcca acgtttacgt attggcaaat gctcgtgatc aagcaagctt gttgtttgat
     4501 aaggccgcag aaatggtcaa acaatcgccg gcgctcttta agaaatttgg taagcctaaa
     4561 cgatcaagta ttaattatgc tcccgccttt tctaaaatgg aaccacgcgc ctcagatagc
     4621 cggaaattgg atgggctaaa cactcacttt ggtatttttg acgagatcca cgagtttacg
     4681 aattacaatc tgatcaacgt tatcaagaaa tcaagaggaa ccagaaaaca gcctctgata
     4741 gtttatatca caactgctgg atatgtatta gatggtccgt tgatgtctta ttttgagcaa
     4801 ggtgtggact gtttggaaca tttggaagat gacatcgatg aacggacttt ctattatctg
     4861 gcaaaacttg acagtgcgga agaggctgat gacccaagat tatggatcaa agccaatccg
     4921 aatatttgtc taatgaattt tgttggcatg ctagatgact atgttaagga taaaaaagat
     4981 ccaaaagaat atgctgactg gattaccaag caatttaact tgttttccga tatcgatgag
     5041 ctgtcatttg tcgatatgcc taccattaaa cgaaacaata aaaccatcga tattgaaacg
     5101 ctcaaaggta agaagtgtgt cggtggtttt gacttgtccg aaacggaaga ctttaccgca
     5161 gccgttttag aatttccgct tgaaacaggc gaggtattca ttttgcaaca cacatggatc
     5221 ccacaagcta gatttgatcg agataacaat caagagcgta tcaaagcgtg ggagaaggtg
     5281 ggagatctaa cgattattcc tggtgattac gtcaattatg aatacgtctt aaattggttt
     5341 gtagaaaatt cgaaaatcta tgacattgta aaaatcaatt atgacaaggc caaggcgcta
     5401 cgattaaata aagaactaga aaatgcagga tttgaaacca acgagattcg gcaagggttt
     5461 ctatcattag gtgggccaat gcaaaacttc aaggaaatgc tattggacgg taaggtgatt
     5521 ttcaacaatt ccaagcttta ccgatggtat ctatccaacg tcaagctggt gatggatcgc
     5581 aactcaaact ggatgccgtc taagcagtcc aagagtagaa aaatagatgg ttttgcagca
     5641 agtttgaaca gccacgccga agtgttgaat atgttggtta atcctgtcgg aaccgggaaa
     5701 gtaacctatt actcgatttc cgatttaatg aatatgtaag aaaggtgtgg aggaatgagt
     5761 attttagatc gtttgcgttc ttttggccga gcgaagccga aagcgagcaa acaagagtat
     5821 tttttgaatg acccgggatt gataccgtat ttagtcggaa aagatgaaat atcagaaggg
     5881 attttttccg taattagccg tgtatcgaac gtttttgcgt ctctccctct caaaatgata
     5941 gatgtggagt ttggccaacc ggacgactgt cctgcataca acttgttgag cgaaggccct
     6001 cgatatttta caaagtttga ttttttccgg gacgtggaag ttttgagaaa ctaccaaggg
     6061 aatgcgtatg tgcagatttt ccgaaatatc aatggagaag tagcagatat ggcgttagta
     6121 aaacctggtg cttgccatcc agtgattgat atggatagcg gggagcttta ctaccaagta
     6181 actgcgactg acaaaggcag ttacaagcaa gttatctatg tacattacat ggaaatgctt
     6241 cactttaaac aaccgaggtt tggcggcttg gaaggtacag accccacaaa agtattaacg
     6301 aataccctcg gatatgatcg agaagtccga aaaatctctt taagtcagct taaaggaagt
     6361 aatgaagggc taaaagttaa gtttgctagc aatatggatg aagaagctaa aaaagctaca
     6421 gttaaaaaca ttgctgattt ttatcgacaa aacggtggac tacttgtgga agaaaacggt
     6481 gtagaaatcg aacgtttaca acgagagctg gtagacagca agcttttaga tactgataaa
     6541 gtatctcgct ccagaatcgc tatggtctac aatgtgccgg agcatttcat cgggaataac
     6601 cagtcgagtt actcctctca ggaacagctc aatatggaat ttttgacgta caatctagta
     6661 ccgaccgtta atcaatatga agcggaacta aataagaaaa cactatcgag aaccgaaaaa
     6721 gcaaaaggtt atcgatataa gtttaatgtc gcaagtttgc taagagctga cactcaggca
     6781 aggggacaat tctatcaaat catgcgacga ggcggagcat actcagcgaa tgatgtccga
     6841 agatttgagg acttgcaacc gataaataaa accggtatgg atgattacca tatttccgga
     6901 gacctatatc caatcgatat ggatccaaca ttaagaaaaa caacctcgtc taaaagcgta
     6961 gccgaaaacg gttaggtttt tttagtttgc accgaaggga ggtggaaaaa tgaaaaaagt
     7021 gacgttaagc ggcgatgtcg tggataacga taccgcgtgg ctttatgact ggtttgggat
     7081 caattgtatc tcaccaggga aaatttctgc cgctcttaca gaagcagcgg gggatgaagt
     7141 agaacttgat atctcatcga acggtgggga tgtcctagcg gcaagcgaaa tatataccgc
     7201 tatccgtgcc tatccgggaa aggtatccgg aaatgttgtg agtattgcag caagcgctgc
     7261 aagtgtaatc gcttgcgctt gcgaaccgct tagaatctca cctacggcac acatcatgat
     7321 tcataacgca tgggtgacca ctagtggcaa cgctgaggaa ttaaaagcca atgcagaaat
     7381 gttaagcagt gtggatgagt ctattgttaa tgcttacgag atcaaaacag gactcgatcg
     7441 gaaaaaactt gctgatttaa tggcgaaaga tacttggtta aatgctcaaa cagcagtagc
     7501 agaagggttt gcagatgaaa ttatgtttgc agaagcacca gtaacggtac tcaatgcctc
     7561 tcaaccggtt attccaaaaa acgcagtaac taagttgaaa aatttaatac tcaaagcgga
     7621 agcaccgcaa gaagaaacac tcttacagaa aaaactaaaa gccttaaatg gagggaaaaa
     7681 cgaatgaatt tagaacaatt aaaaaatgcg tgggtcgagg cgggaagtaa agtctctgac
     7741 ttaaatgcac aactcaatgc agcattggtt gacgatgaaa aaacagaaga agatgtagta
     7801 agtttgcaag cacaagtaaa agcagcacgg gctaaacggg acggattgaa agagcaagtg
     7861 gcaaatatgg aagccgaaca agtcttaaac gtcaaaaaag aaccattaga taaaaaagat
     7921 gaaaacttga aaaacaagtt tatcaaagac tttaaagcga tggtcaatgg tgatcctgct
     7981 attatggcta ctttgacatc taatacggat gaatctggta atgctatcgg attgactatt
     8041 cctgtagatg tgcaaacgac tattcatact ttggttcgtc ggtttgactc tttgcaagaa
     8101 tacgtaaacg ttgaaaaagt gaccactacc agcggttctc gcgtttatga aaaatggtct
     8161 gatattacac cgttgactgc tttggatact gaagacggtg aaatcccagc aaatgatgat
     8221 cctgcacttt acttgatcaa atacttgatc aaacgctatg caggtatttc cacagtaact
     8281 aacagcttgc taaaagatac tgccgaaaac attttggcat ggttgtctaa atggatcgcg
     8341 aaaaaagtag ttgttactcg caatacaaaa atcttggcag ctattgatgg aatcaaagcg
     8401 gcacaaaaga aagatgttac agatgttgat ggaattaaag atatcgtaaa cgtccaactt
     8461 gatccagcta tcgaagctac atctatgttt attacaaacc aagatggctt caatgtttta
     8521 gataaagtga aacgtgctga tggatcttac ttgttacaaa aagacgtagc ttctgcaact
     8581 ggatatacct tcttaggtaa accaatcaag aaaattgctt ctcgtttctt gccaaacaaa
     8641 ggtacgcaag ctactcctaa atatccactg tacattggtg atctgaaaga agccgttaca
     8701 ttgtatgatc gcgaaaacat gagcttgctg acaacgaata ttggtggtgg agcttttgaa
     8761 acagacacca ctaaagtacg cgtcatcgat cgcttcgatg tgcaactagt tgatgatgaa
     8821 gcggttgttt tggctacttt tacaactatt gcgaacgaga caccggcgaa agtttaagga
     8881 gctgattcct tatgattctt gatcctaaaa tggatttagg cgaaatcaaa aacgcactaa
     8941 agattgatac cgatgatgac gatgtggaag taagccgtgc ggcacaagct gcaattgcat
     9001 acattagagg ggctatcggg aatgataagc cctcttttta taagcaagaa aatgaaacgg
     9061 ttgatctgat taatttagct atcctgcaat tagcagatca ttattacaac gctcgatcag
     9121 caaccgtgag tgggaacttg cgagagtacg atttaggttt tacaagccta atcttgcaac
     9181 tcaaagcaag ttatttgctt tttgtggagg aggagtagcg tatgcccctt atccaaacag
     9241 gaaatttaaa tcaacgcatc aagtttgtcc gagatacgac ggttaaggat gaggacgggc
     9301 aagttgtccc gacttctaca accattctta cttgctgggc aagtgtgcag acacaacgcc
     9361 tgaacgatat taagacgtcg attggtacgg ctttggaagg aacactgacg ttcattatcc
     9421 gctaccaaca aaaatcagag ctgaccaatg atatgaaagt gcgttggaat ggaaaaacgt
     9481 ttgaaatcat tacgattacg aaaggcgagt ttgcgaagga cttcacgaca atcattgcaa
     9541 aagaggtttc aaaatgagtg tagaagtcga tgcaaccgaa gtgtacaaag cgcttaggga
     9601 agtaaaagca aacgttcaac gagtggaaag cccagcactt agaaaagctg gggagtacgc
     9661 tcaagaagag ttacgacaaa acacacctta ctgggatgga acgaagtcaa acggtaaacg
     9721 tggttcgtat atgcaagaac atgctaagaa ccatgtggtt acaagctcga taaaaaacgg
     9781 attggtagaa gtcggctatg acaaagatgt ttcttggcgg atgcacttta tcgagttcgg
     9841 aacaatcaaa caacgtccaa aaggtttcgt acaaaaaaca caaaagcaaa tcgaaaaaca
     9901 agtaacacaa atcattgctg acgaagtaaa aaggaggcta ggactttgaa aacggcagta
     9961 tcacaagtct attcaattct gaatagcaat gaaaaaacaa agaacattga tttttacacc
    10021 aatagtgttc cggaatcagc tcaaacagta cctagccttc cagttggcag aattacagag
    10081 atatccggca actatgaaga ttttgcaagc aacaatcctt tgaccattca atttaacgta
    10141 caggtagatg tatgggtgtc aaccatgaaa gaggttgatg ccttttattt tgcccttgat
    10201 gaggttatga gggggaatgg ttggcaatgc gcatacacgg aacaaacaga tgacgaggac
    10261 ttggaaggtg caaagcggat tatcaaacga tatgtagcaa atatttcact aaattaaaag
    10321 gagagaaaat agatggcaac agtaggattc gagagcgtca tttttggcgt aaaaacaggt
    10381 gcaggcggca ctctaaaaga attagtagca gataagtcga aaggcggagc gatcgaagct
    10441 aaaattactg gattaggcgc aacttctaac acaacatacg cttcaaacgt accgttcttc
    10501 attgcaagta aaggggtttc gtcgccaaaa gttacgcttg acgtggcaga cttaatggat
    10561 aacggcattt acagcgaaat cattggtgct aaaaccgtgg atggtgcaaa tgtaattggt
    10621 tcagaaactg aagcgcctta cgtgtcggta gtcatggtta cagcgaacaa agaaggaaaa
    10681 cgcttgttca tgggattgac aaaaggaaaa ttcagtcatc cagatatcga catgaaaaca
    10741 gctgaagaca aaggggtaga attgcaaacc gattccatcg aaggggaatt catttctgat
    10801 gaacgtggct atgtatactt aacagccgta gaatcagaag gaatgacctt acaaaaattc
    10861 aaggacttgg taaataacaa agagggggag tagttaaccc tgcatctaca ccaacgacag
    10921 atacagggac accaaaagaa ccagaaccaa aaattgatac acaaggttag ccatttttgg
    10981 ctagccttat tttttgtaaa aacaaggagg aaaacaaatg attgaattgc aattgaaact
    11041 tgacggaaag aaaaaaacat tcaaacaaca agatatttcc gcacgtgcaa tgcgtgagtg
    11101 tatcaaattt tacgagaaag cggaaaaagc agacctaact gatttagaag cgattgattc
    11161 aatgattgca attacagcag atattttcca agatccagca gttacatttg atgctatttt
    11221 agacggtttg actgcgagcg agttagtacc ggcattagaa agtgtttttg aacaaataaa
    11281 tgaactggga aacaatgaaa aaaagcagac ggcgagcaaa aagaaataag tttttctgaa
    11341 gctaggaaag caatggatca aatctacaaa gatttaatcg aagcaggttg gacgatgaga
    11401 gatgtggacg aagccgacta tcattatttg ttacaccttt ttggagaagt ggagagtggc
    11461 gaagaatatg tagatggtgc tgatttcatc aaacaatttt tatcggctga agacttagta
    11521 aaacttgagg aaggaggtaa ataatggcag gaaaaggaca accggcagga aatatcaagc
    11581 tagggattag tttagatagc actagttttg gtaacacgct ggacgaaatc aatgcgaaag
    11641 tcaaacaagc tgagtcgaat atgcgtgcca atctaaaggc ttatgattca gcaggacgtt
    11701 catacgaagc acttagtcaa aagacgaaag acttgtctac ggttatggaa gggcaaaacg
    11761 ccaaagtaag agaattaaca aagcgccgtg atgaagcgat tagcaagtat ggcgaggaat
    11821 cgaaacaagt tgctaacctt aacacacaga taaacaatgc taccgcaaaa tataatgctt
    11881 acagtcgcca gttgaacgac acaaaaaaag aattggtgta ttccaaaaca gccgtcaatg
    11941 atttatctaa tgaaatcaaa gaaaatgaac gacaaatgaa cgccgaagta aaagcgttga
    12001 aagccgctgg tgatgaatct ggtgcttttg aagcaaaaca aaaagggcta gccaaacaaa
    12061 cggaattatc cgagaaagct atcgaagaac agcgcaaagt tgtgaaactg atggctgatg
    12121 agtttggcga ttcagcaaat gaaaccgaag atgcaaaaag ggcattagaa aagttagaac
    12181 gacaaagcca aatatctagc aggcaattag aagcactcaa aagctccagc gatcaatcag
    12241 gaaaagaaat agaagatttt ggcgacaagt ccacaaggtc agctaggaaa ctggacggac
    12301 taaaagacaa attaagctcg ctaaaaagcg cattttcgtt tggtgcagtt gctggattag
    12361 cgcataacgc tattagcagt gtagtaagtg gcgtgcaagg tttggttggc gaagcagtaa
    12421 acgcatcgga ttcattgatg aagttttcca aaaccatgga gtttgctaac tttgggaagt
    12481 cacagataga aagctcgaaa aaagaaatga aagactacgc cgataagacg gtttatggtt
    12541 tagaagaaat tctgaacaca accgcacaat tggcatctaa tgggattcct aactatacag
    12601 aactaaccaa ggcggcaggt aacttgaatg ccgttgcagg cggttctagt gatacattca
    12661 aatccgttgc catgatgcta acgcagacgg caggagctgg gaaactaaca actgaaaact
    12721 ggaatcaatt agcagatgcg ataccgggtg cttcaggatt gttacaagac gctatgttga
    12781 aaaacggagc ttatacagga aacttccgtg atgcaatggc gcaaggtcaa atcacttccg
    12841 acgagttcaa ccaagcaatt gtacagttag gtatgaatga cggagcagtt aaggcagcca
    12901 cttctacaga cacattgagc ggttcttggg agcagatgaa atccactgta ataaatgggc
    12961 tacaaagtat tatagaaaaa ataggcgttg aaaatatcac tggtttcatc aacagagtaa
    13021 caaaagggat tgaaaattct attcctaaaa ttactcaatt tataggttgg ttgagagata
    13081 ttggaacgtg gatcgttgaa aatagagagc cactaacatg gattgtcgga atcataggcg
    13141 gaattacatt agcagtaaaa gcattgaacg tagcaagtat gttgctggca attactggcg
    13201 gaacattggc agcccctttt gtggcgattg gtgtagcatt aggcgcacta gcaggtgctt
    13261 tggtgatagc ttatacaaaa tctgaaacat ttagaaatat agtcaatgcg gcttttacag
    13321 ctgtgaaaaa cgtagttatg agcgttatca ataatttggt ggaatactac aaaatgttgt
    13381 ggagcgtgtt gcagtggctt tgggaaaaaa taaaagaatg ggcttcatgg attggcaata
    13441 aattcattga aatgaagaac agcgttgtga atacggttca gaacctatgg aatggcgtga
    13501 aaaacttctt cagcaatggc gttggagata cgtggaataa ggtagtcggt tgggtaaaaa
    13561 acattttcaa caaagcaact gaattgaaga acaacgtttc tgatgtaatc ggtaacctgt
    13621 ggaatggtat caaagacaca ttccgtagag gtatcgatac ggtattcaat tggttttcag
    13681 aactaccaac gaagatgaag aatgccatta ttggcggtaa aaacgccatt gttgatgcgt
    13741 tcaaaagtat tttcaacgca gcacttaaag cgataggtaa accagttaac gcaatcatcc
    13801 atggagcttc atgggtacta gaaaaattgg gcgcagaacc tctacaagaa tgggatgtac
    13861 cacaatacgc tacaggtaca ccagcaggtg ggcacccaat caatggtcca atgatggtta
    13921 atgatggacg tggagcagaa acagttatca caccagatgg tagagccttc attcctaaag
    13981 gacgcaatgt tgttctaaac gcaccaaaag gaacgcatgt cttgacagca gaagagaccg
    14041 cgcaacttca aggttctaaa gctcctaagt atcgttacaa aaaaggtact aacttctttg
    14101 gtaacatgtg ggatagtgtg aaaaaggttg ctggtaatgt aggtcacaca attaaaaacg
    14161 tagtaggtga cgtgtgggac tttatttcag acccgggagc gttagctaga aaagtacttg
    14221 gggggttaga tgtattaggt ggattgacaa agtatccatt agaagtaggt aaaggcatcc
    14281 tatctaaagc aacaagtgca ctgactgaaa aaatcactgg gttgttctca tctggtaact
    14341 tagatacctc cgtaggaaca aatggcgtct ataaatattt ggctgatgtt gctaagtctg
    14401 tgatgaagaa attcccagga tttgtggcaa ctagtgggta tagaccaggt gacccttatt
    14461 cacatggtaa acgtaatgcc attgatattg cattaccagg cgtcacagga ggctcaccta
    14521 gatacacaga agcagcaaac tacgcttttg acaaattcgc ttccaaaatt ggttacgtaa
    14581 tcactaatgg gaaagttcgt gaccgttcag gacaatcagg tcaaccagca actggtgcat
    14641 gggagccatg gcccgatgga gatcactatg atcatgtgca tttaaacggt gtgaaagacc
    14701 cacaaaacac tcaaatttca ggagatagcg tgggaggcag tggggtagaa agatggcgca
    14761 atgtagcaat tagagcgttg aaaatgaccg gtcaatacag tactgcaaac ttaaatgcat
    14821 tactaaatca aatgcgtaca gagtcaaatg gtaatcctaa tgcagttaac aattgggata
    14881 ttaacgccaa aaatggaaca ccatcaaaag ggttgctcca agtgattgac ccaacattca
    14941 gacagtatgc aatgccagga ttcaacagca atatttttga cccactatct aacatcttag
    15001 cttcaatcag atacgcacta tcaagatatg gctcactaac aaatgcctat cgtggagttg
    15061 gttacgcaaa cggtggaatt gtaaaccaac atcaaattgc ggaaatcgca gaaggaaaca
    15121 agccagaaat tattattccg ttagataagg ctaaacgatc aagagcgatg caattattgt
    15181 ctatcgcaat ggacaaacta ggagtttctc ctaaaactta tggaaatgcg acaactgttt
    15241 ccagtgatta cgaaatccta gaagccattg aaaaacaggc ggcaacaacg aatcagctgt
    15301 tatcgttatt gctggcattt ttcaaaggaa atagccgaac tgatagagat ttagctttag
    15361 atattcagaa gatcttggtt aggaggatgt agatgcgaac tgtattactg agaaatagaa
    15421 taaacgaaga aattgatttg tccacagaag actattttgc tactgggttg agtaatatgg
    15481 ggtttgaagt aaaaaaagaa catgtgggac agtgggggaa tttcagagaa agcagtgaga
    15541 gcgttgaaat atctgaattt cagtcatctg taatcatttc tgtgcatggg tttcgagaaa
    15601 aagaactgta caattcactt gtgcagttcc tatcggaagg tccgtttgaa ctggaatttg
    15661 cttttgacgg cgaaacaatg gtaagaagat gcagtctaaa atctttaagt aagactgaaa
    15721 tcgatccgaa aacatcgtta ctaacagaca ctttagaact atatttcaca tcaaactggt
    15781 attccgtaaa acgcgaaaaa ttaattcagc gaccgaatgt agtgaaaaca cgtggtaagg
    15841 tttttccgta taaaagatct tatatctaca ctcaaaactt gtgggagaaa aaaggtgtat
    15901 tcaaattcaa taataattct gtatacctaa cgaatagcaa ggaacggatg tccccgctaa
    15961 aaattcgtgt gattgggaag tgttcaaatc cgtattggga agtaatccaa aactcacaaa
    16021 tcatcgcaac ggatggatac ttcatagata tgactgaaac acagactcta gaagtatcaa
    16081 gcctttttga agatacaacg gcgattttaa aagatattgc aggggtagaa tcttctgtct
    16141 accaacaaca ggattataca aaaactaatt ttgttcaggc cccaactgga gaattcagca
    16201 ttgtgtttca tgttgggggg gcagacgtag agattgagtt atacgaggag cgtgatctgt
    16261 tttgatttta gcagtcacgc tttttcatcg tgatttaatg ctttataacg aacatttatt
    16321 ctctaacagt tttgaatttg gtgtggacga gatcaacgaa gaggctagta gctttacgat
    16381 ggataagtac gttcctgtaa aaacaggcga ttttctacta gcaaaatata ttcctagtgg
    16441 aaaatttgca tattttggtg taattacgtc gcaggaagac gaaaaaatca gttgtaaaag
    16501 cttacttagt ttagctgata gtgagatacc gactgcacgt gtgtcaggag ataactacga
    16561 agagcatatc cggcggttga ttgaatacta tttgctgaat gatccaacga aacaactaaa
    16621 agatatttta gacgtcaaag cagaaagtgc gacctctcat tcgtatcaag ctaccgatac
    16681 gaataaacac aagttaagtg cgtatattct caatggtttc aaaaaataca acgtaaaatg
    16741 gtatttcaag ggaattcaaa acagaaaaat ttatacaggc atacgtgctg taaatgaatc
    16801 aatttacatt aaagacaatt cttctgaatt tagcgattgg gatgtgtttg ttcaggcgcc
    16861 gggcgctgga aacgagaaca agctattaat cgttgataaa gcaatgaaag atatagagaa
    16921 accgataata ctgtcaacat ggtatttgga cgaagaaaac aatttgacac aagatggatc
    16981 gaaagaaaat atcacgaaac caactgtcaa tttggttaat atctacgatc aaaccgcaga
    17041 agataaagca tcctacgaag atgtggcaaa atcagagttg aaaggcaata cgtattcgca
    17101 tgaaatcaaa gtgaacgttg taagaaatgc aaaaaatttg aatgtcgaaa cgattgaaac
    17161 agggatgttt gccacgattt cttataaagg aaagatatac aagtcggttt tgacagcttg
    17221 gcgaatatca agtgataagg agtttgtgga attgactttc ggaaacataa gaagtcgttt
    17281 tatggattat tttgaagata atgggggata aaaaatggtt agcaatgtgg atggatatca
    17341 atttgaaaac gtgaaagtaa gcgcagaaaa tgatgctaga ctttatcacg ttttatataa
    17401 tcggaaaaat caggttattg atggttacga tcagtctatg aatttatctt caagcggatt
    17461 aacagtaaaa gttgctgcag gagcagcgat tattcaaggt cgtatggtcg ttgttcgaca
    17521 agaagaaagt ataacagttc cagcaaactc aagtggttat atagcattaa cagtggattt
    17581 gacacaagaa gttatacctg gatctattct tccagaatcg gaagaatatg aatggactaa
    17641 taatcaagtc aagctagaat ttataacaaa agttataaaa ggtaatttaa acaatggtga
    17701 taaggtctat aatttaccac tatgctcagt taattctact ggatcaactg tttcaatctc
    17761 aaagatatcg gatagttacg agctgactct ctctaaagga gaaattttgt ggagggggac
    17821 tgcgttaatg catgatactc aaactgtcca accttcaaaa aaaatttggc agacagtttc
    17881 aggttttttg ttagtatggc tcccatacga aaacgggcaa gcaattgagg atagatatgt
    17941 aactacgcct ttttataagg agcgtgtaac ttttactaat gttttaggag aaattgtttc
    18001 aggatttgat gactatcaca aaaagtggtt tagtaaacga ataaactata attcgaatac
    18061 taatatattt acaggtgcag caagcaatgc aagtggagat aacgcaaata tggtgcttag
    18121 gtatatagtt tctttttagt taggaggtat ggaagagtgg caaatttaga aattaaatta
    18181 tctgcaaata agagacagcc ttatctacgt caccgtgttg ttggtagagt tggtgatggg
    18241 gggctcacaa caatcaatgt acaacttctt gaggaagatg aaattacacc ttttgtaatt
    18301 aatctaaacg gtactttgaa atttgtgggc gaagtttcaa acggtaacta taccgaggga
    18361 gaaccagaaa taatcgattc gactaatggg ttaattagtt acacgttcac taagtcaaat
    18421 ttcagcacga gtcatcaatt caaacaagca tattttgaat atgtagatcc taacggcaaa
    18481 aaagtaactt ttcagaactt catcatagac gtgttagaac gagtggatat taattcggag
    18541 caagcgaaat actatatttc ttcattggaa aaattacaga gtgaaatgca aaccactttc
    18601 aatcagttca ttagtgataa acaggtccaa tacgatcaaa tctactcgaa atataacgaa
    18661 ttagtaagat tgataaatga atcagataag caagtaaatg atcgtattga tcaaaccaat
    18721 cagcaaatcg gcgatctcgg caagctgaaa aagatgtaca gtaacagcat cgactttggg
    18781 ggctatgatt attcggggaa tccgaatttg ttaagtaagc tatcatacga cttaattgaa
    18841 aatcaaaata cttcagctgg aacactttct aagggtgaaa actcgtttaa atataataag
    18901 atatcagctg aagtggaagg tggagtagag ttatattata aacgaagagg tatagctaac
    18961 tggttaccct ctaataaaac gcttgtaatg actgttaagc ttagagctgg agcggactat
    19021 agtccagttg acggaaaact tatactgatt agatataggt atgttgacag tggaactggc
    19081 aagattgttt tagacttacc tattaacagt aattcgataa ctcaggaatg gaaagagttt
    19141 agtattactg gaactactcc aacatttagc ccacaagcat accatccttg gatacaattt
    19201 agggctcaag atggggtact tggggaaata gaaatgagct atgacatcaa aatcgaagaa
    19261 ggctctacag ctacaccata tcagcctaac ttacttgatg actcttactg gctaggtaaa
    19321 acaccgttgg gtgagaattt gataacgaat gccaagttcc caattataac acgaagcaat
    19381 cctatttctg gttttgatat ttcagaagaa ttaataatag gtgaaactta tacggtatct
    19441 ttaaaaggga ctaagccagc tactaaagaa ttccacttat actacggaga agcaaattac
    19501 cagcagttgc agtatcaggc aactttactt cctgtggaag gattaacaga tgtgtggagt
    19561 gccactttta cagccagaaa tactagtgaa acgacaaatt tacttagagt agcgttatgg
    19621 caaaaaccta attctgcaac atatgatact gttcaaattg actggctcaa gattgaaaaa
    19681 ggcgacacac gcacaccaaa tatcagtcag tttaaatact ttggtgaagg cttgaaagac
    19741 agtaacaatc cgaacgacta cagctgggat gtcacaccag agtatactga aaaaagcttg
    19801 aataatacgg ttagtctgac tgaaccgcaa tccatcgaag gattgaaaaa tttcgaagat
    19861 ggtattcaat caaaaggaaa atctgtattg acatcagacg acaacaaata tgaagtcgta
    19921 accttaacag ttacaaacgg gaataccgga tcagcaaagc tttatcgtga aggaaaaacc
    19981 gtcactattt atttttttgc gttgaatggg aaaagcagtg gtggaaatga ttcaacgata
    20041 ctaacaattc cagaaggcta tcggccacca attagttttg agcaactggt tggctcgata
    20101 gaccgttcta ctttgaacag tgctcagtta tctattggtg cagatggagc cattaaatgg
    20161 cgaagaaact caagttatgg atcggattat acctttgcaa ttacttacac gatttagaaa
    20221 ggcgtgaatc gatatgaagg cagcatatag accaattgaa ccttacggat tcgagcaaat
    20281 cattgtgaat gatgaagaac atttaccgga agaatgcaca gaagtcgaac caccgattcc
    20341 aaattggaaa ccgaaattca attactggga gggaaataaa tgaaaaacat ttggaaatat
    20401 ggccgtactg gcggagagta tgcaggaaaa gtattggacg acatgcttgt atccgttcct
    20461 tacacagatc agccaccgct cgaaggggtt cgttcagatg gcgaaccgct aacgattgct
    20521 gatcagatgt ttgatcctaa attgaaccaa tggattattt tagcgaacgc gttagatcac
    20581 aacgatttaa acaatctcaa agcgatgtac gaggctcttg aacatgaaaa cggcaaccta
    20641 aaacagctaa atgccaaact catgctaagc gatgtagcga ttaaacagga aaatactgca
    20701 ttgaaagaaa aagctgacag tttagcacaa atcaattcaa aaatgatgct tgcttcgtta
    20761 caaaatagca aagacatttc agaaattaaa gagcaactaa atccagcttc aaagggaggt
    20821 gagtagtatg tttagtttta gcgatgtgaa aatgatgtat gattggggct gttttactga
    20881 cgatcaagtt cgtctattcg ttccactatg tatcacagat gaagaagcag atagaattat
    20941 tagcaaagaa gagagcgcat cttaagtgat gcgttttttt gttggaaagt tggtggaaca
    21001 tgaaagaaga agcgctccaa gacgttgtgg agagattagt aagaattgaa acaaaattag
    21061 acaactacga atcacttaga gaaaaagctg atagtgcaaa agatttggca gacaaagcct
    21121 actcagtagc gctaaacaac gcagaagata tcaaagaaat gaaagccaat aataaatggt
    21181 cgtggggtta catgatcggt ttaggcatta cgatcattgg ctatttcttg actaaattgt
    21241 aaaggagtta agaagaaatg attttacccg ataagtatta tcaagtcatt aaatggacgg
    21301 ttttaacagt attaccagct gcatctgttt tagtagccac gttaggaaaa gcgtatggat
    21361 ggaatggaac agatatgaca gtactcacta tcaatgcagt agcaacattt ttaggcgtta
    21421 tcactggtgt gtctgcatat aacttaaaaa aataggagga aaaaaatgaa gaagaaaatc
    21481 attttatcat tgagcctaat aatggctctt tttttattgc caattaacgg gttcgcctat
    21541 acgatcaaca atgaatttaa tttaggtgta aatgaaggta gctcacaagt agcaaataac
    21601 caatacatcc tattgcatga aacagcaaac gaaactgcga caggacgaaa tgaagcgcag
    21661 tacatgaaac gttcttggta caatgcctat acagcgtata ttgttggcga tggtgggatc
    21721 gtttaccaag ttggacaacc tggttatgta caatatggcg ctggttcata tgctaatgca
    21781 aatagtcctg tgcaaatcga actgcaacat acgcacgata aagtaacgtt tgaaaagaac
    21841 tacaaggcat atgttgaatt agcgagagat tccgctaata aatacggtat tccacttaca
    21901 ttagacacac cgtataatca accaggaatc aaatcacatt tatgggtaac tcaaaacatt
    21961 tggggcgatc acactgatcc gtatggatac ttatcagaaa tgggtgtaag taaggaaaaa
    22021 ctggcttacg atttagctca tggatttaca gacgaaaacc caacgacttc tgagaacaag
    22081 cctgtcattg atccaacccg agcaggtgca gcaaatccaa ctttatcaga tggaacgaac
    22141 cattctcaca ttgatcagtt tggagaaatc gaaaatgcga acttacacgt cgctggatgg
    22201 cacatcgcta actataaata cgagtatatt ttcattatgg actacaatac tgggaaagaa
    22261 ttagctcgag taagagctga tggaatttat agaccggatg taaatcaagc ttataatact
    22321 tctggaaacg ttggctatca tgtatctttt aacatgcgca atttccctaa taagaaagta
    22381 tacgtcatga tgcgggcaac gaatgatcca gaaggaaaca ctaaaggcgg tgcgcaagat
    22441 ttccatgaca aacgttggta tttaaatatt cctaaacgat aaaaatagct cctcgttgag
    22501 gagcagtaca tagaattgaa aattaacgtt aaatcaaaaa aatatttaca agatgtttat
    22561 ttaagtagat aataaaagaa aaaagcaggt gaaattgtga cagcagaaat tggtattatg
    22621 aataaaagtg gaatcgtgtt agcttcagat tcagcatcta cgattggaga tagcaaagta
    22681 tataatactg caaaaaaatt atttactttg gattccatgc attctgtagg tatcatgata
    22741 tatggtaatg ctgaatttaa tggtattcct tgggaaataa tcattactca atataagaaa
    22801 agcattggta gttctgtttt taatactttg gaagaatacg cggataattt tatagaattt
    22861 gtcaaaacag catcttttat tagaagtgaa cagaccgagc aagaacaaat gattggggtt
    22921 tttcaaaaaa taatttcagg tttatttgag agtatagaag tagatattaa ctttttaata
    22981 agccaaggta cacaaataga caaagatgtc ctagtgaagt tattacaaac aaaaatgaat
    23041 accaatttgt ctcaacagtc ccagacattc attttagata ttgaaaaggc gttatttctt
    23101 agtaactatg gagaaatatt aaaagatatt cttaatagta tttctacaat ggaaggtgtt
    23161 tcagaagcta tatcagagga aattcaaagt tacgtttatg aaattataat tagagacgat
    23221 gtttattctt cccctacagg aatagttata gcaggatatg gaa
//
LOCUS       DAYYBG010000040        23105 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000040 DAYYBG010000000
VERSION     DAYYBG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.040"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1184
                     /locus_tag="ACHAUN_001484"
     CDS             <1..1184
                     /locus_tag="ACHAUN_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM8460188.1"
                     /translation="QAQFIKGRLDDSPSHCEHCGSIRIIRNGSYTTRTQMLKVKEKLT
                     ILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFV
                     SDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTNQLFTIL
                     EDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHI
                     NRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPI
                     SQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRDTEGFQETLKTISSTLPQSFKKKFT
                     IFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFE
                     N"
     gene            complement(1299..2795)
                     /gene="lysS"
                     /locus_tag="ACHAUN_001485"
     CDS             complement(1299..2795)
                     /gene="lysS"
                     /locus_tag="ACHAUN_001485"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8460189.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2906..3910)
                     /gene="dusB"
                     /locus_tag="ACHAUN_001486"
     CDS             complement(2906..3910)
                     /gene="dusB"
                     /locus_tag="ACHAUN_001486"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8460190.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3911..4798)
                     /gene="hslO"
                     /locus_tag="ACHAUN_001487"
     CDS             complement(3911..4798)
                     /gene="hslO"
                     /locus_tag="ACHAUN_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8460191.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(5015..7126)
                     /gene="ftsH"
                     /locus_tag="ACHAUN_001488"
     CDS             complement(5015..7126)
                     /gene="ftsH"
                     /locus_tag="ACHAUN_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8460192.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(7216..7761)
                     /gene="hpt"
                     /locus_tag="ACHAUN_001489"
     CDS             complement(7216..7761)
                     /gene="hpt"
                     /locus_tag="ACHAUN_001489"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8460193.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7761..9206)
                     /gene="tilS"
                     /locus_tag="ACHAUN_001490"
     CDS             complement(7761..9206)
                     /gene="tilS"
                     /locus_tag="ACHAUN_001490"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8460194.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(9326..9796)
                     /locus_tag="ACHAUN_001491"
     CDS             complement(9326..9796)
                     /locus_tag="ACHAUN_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8460195.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9842..10267)
                     /locus_tag="ACHAUN_001492"
     CDS             complement(9842..10267)
                     /locus_tag="ACHAUN_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8460196.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(10334..10603)
                     /locus_tag="ACHAUN_001493"
     CDS             complement(10334..10603)
                     /locus_tag="ACHAUN_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8460197.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(10614..12209)
                     /locus_tag="ACHAUN_001494"
     CDS             complement(10614..12209)
                     /locus_tag="ACHAUN_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8460198.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(12224..15745)
                     /gene="mfd"
                     /locus_tag="ACHAUN_001495"
     CDS             complement(12224..15745)
                     /gene="mfd"
                     /locus_tag="ACHAUN_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8460199.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(15762..16322)
                     /gene="pth"
                     /locus_tag="ACHAUN_001496"
     CDS             complement(15762..16322)
                     /gene="pth"
                     /locus_tag="ACHAUN_001496"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8460200.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            16675..17649
                     /locus_tag="ACHAUN_001497"
     CDS             16675..17649
                     /locus_tag="ACHAUN_001497"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8460201.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17819..19219
                     /locus_tag="ACHAUN_001498"
     CDS             17819..19219
                     /locus_tag="ACHAUN_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8460202.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(19297..20019)
                     /locus_tag="ACHAUN_001499"
     CDS             complement(19297..20019)
                     /locus_tag="ACHAUN_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8460203.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(20098..20604)
                     /locus_tag="ACHAUN_001500"
     CDS             complement(20098..20604)
                     /locus_tag="ACHAUN_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8460204.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            20727..22526
                     /locus_tag="ACHAUN_001501"
     CDS             20727..22526
                     /locus_tag="ACHAUN_001501"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8460205.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(22594..>23105)
                     /locus_tag="ACHAUN_001502"
     CDS             complement(22594..>23105)
                     /locus_tag="ACHAUN_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8460206.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6441 a         5068 c         4213 g         7383 t
ORIGIN      
        1 ttcaagcgca gtttattaaa gggagattgg atgactcacc ttcccattgt gaacactgtg
       61 gatcgattcg tattatccgc aacggttcct atacgacacg cactcagatg ttgaaagtca
      121 aagaaaagtt gactatttta gaattaaaac gtacccgttt tctctgccat gattgtggac
      181 ggaccttttc tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga
      241 agcaggcgat tctcatggaa ctgtacgaaa atcaatcacg taaactaatc gctaagaagt
      301 acttcgtctc ggatgggacg gtgacacgga tcttgcgtga agcaacgaag cattaccagc
      361 cacgtatgaa cttccttccg acggtgttgt gtatggatga gttcaaatca atgaagtctg
      421 tttctggttc catgagtttt atttgtgttg atgggacaac caatcagcta tttacgatcc
      481 ttgaagatcg cagactttac aaactaactc aatacttcct gcgctttcct agaaaggccc
      541 gtttaaaggt caagtaccta gtgatggaca tgaatgccag ctattgtcag ttgcttaaaa
      601 cagtctttcc aaatgcggag atcgtaactg accgctttca tattgtccaa cacatcaatc
      661 gcagcttcaa tcaattacgt gtccagatca tgaatcgttt tcgaacctca cactctgaaa
      721 atcagaagaa gtaccgtcgg ctcaagcgat attggaaact cttactcaaa gattccacga
      781 cattggagcc actcaaacgg cattatcatc gactgtttaa acgaccaatt agtcaaacag
      841 aaatcgttga tgagctgtta agctacaacg aagaattgcg aactgcgtat catttttgtc
      901 aattattacg ctattatttc gttaaacgag atacggaagg ctttcaagaa acccttaaaa
      961 caatttcatc tacgttacct cagtcgttta aaaagaaatt cacgatcttc catcgctacc
     1021 aatcgggtat ttcaaatgcc tttaaggtgc cccactcaaa cggcgtcact gaggggctga
     1081 ataacaagat caagctgatt aaacgaattg cgtttggtta tcgcaacttt tataactttc
     1141 gggcacggat ctatttacaa caaggtctga tctttgaaaa ctaaaaaaat atgcggcgca
     1201 cagaacgcgc cgcatatcgg actaagtttt attcaaattt gtcttcgcca acacaatttg
     1261 acgaagagcc aggaattcct tagacagctt ttattcgatt atctcattgt tgggaatagc
     1321 aaaacatccc gaattgattg tgcatcagtt agcaacatta ctaatctgtc gatcccgatg
     1381 cctagaccac cagttggcgg cataccgtat tctaaggctt ccaagaagtc ttcgtctaca
     1441 ccgtgagctt cgtcatttcc tagttcacgt tctttttctt gcgcttcgaa tctttctctt
     1501 tgatcaattg gatcgttcag ttctgtaaag gcattagcaa attcgcgccc cactatgaat
     1561 agttcgaatc gatctgtgaa tcgaggatct tctggatttt tcttcgctaa aggcgaaact
     1621 tcgactggat gtccgtatac aaacgtcggt tgtgtcaatg tttcttcaac aaatgtttca
     1681 aagaattcat tgataacatg tccgacaccc atagcttcgg ttacttctac attgtgtttt
     1741 tcggctaaag ctaatgcttc ttccaatgtc atttcttgcc agaagtctac accagtttgt
     1801 tctttgattg catctaccat atggatgcgt ttaaagtcac tttctaagtc gacagcttgt
     1861 ccatcatatg tgatttttgc tgttcctagt actttttcag ctgcgttacg gataatacct
     1921 tctgtcagat tcattacatc attgaaatca gtatatgctg tgtatgcttc taacatcgta
     1981 aattctggat tatgtgtagt atcaattcct tcattacgga aaacgcggcc gatttcataa
     2041 actttttcca ttccgccaac aattagtcgt tttaaatgaa gttctagcgc aatacgcaaa
     2101 tataaatcaa tgtccagtgc attatgatgc gtgataaatg ggcgtgcggc agctccacct
     2161 gcctcattgt gcaatactgg tgtttctact tcaatatagc cattgccatc taaatagcga
     2221 cggatttcgc tgataatttg gcttcgtttc ataaatcggt caaagctttc gcgattactg
     2281 attaaatcca agtagcgttg tctgtagcgt tgttcaatat tcgttaagcc atggtattta
     2341 tctggcagtg gacgaagtgc ttttgacaaa atcgtgattt ctgaagcttt gatagaaact
     2401 tcgcctgtgt ctgttttcat gatttgtcca gttacaccaa agaaatctcc aaggtctgca
     2461 tgtttgaata cttcataggc ctcatcacct acttggtctt ttcttacata aatttgaatt
     2521 tggccttcac gatcttgtag atgcgcaaat ccagctttcc cttttcctcg gcgagtcatc
     2581 atacgaccag ctacactagc ggtcaatccc atttctgcta gttcttcttt tgttcttgga
     2641 tcaaatagtt catgcaattc ttctgagttg tgcgtgcgtt caaatcgttt tccaaatgga
     2701 tcgattccat cttcacgcaa ttgttccatt ttttgacgac gtacaagcat ttgatcattt
     2761 aaatcttcta cttgatgttg ttgttcctgt gtcacaaaag ttcctccatt ctcatgttaa
     2821 tctctttcct ataatgccaa tgaaacacaa aaaaagcaag ccgaacttgg aatttcggct
     2881 tgaaaattca cgaaaaactt agaaatcaca acgtttgttt caatagtact ttttccgctt
     2941 tttctacaaa ttgatccagc aggtcattga tttcttgctg tttttctgtc tgattgattg
     3001 ctactttcac tttcgctgct cgaggaatcc cttttaaata ataagccgca tgtgttctga
     3061 attctctaca agctactttt tctcctttga gatctgctaa gcgttgtaaa tgaagtttag
     3121 ctgtagcaat tttttcggct ggtgttggtt caggaatcag ttcccctgtt tcgagataat
     3181 gctgtgtgcg gtgaatcatc catggattgc ccagcgctgc acgaccaatc atcacaccgt
     3241 ctgcaccgac atagtcaagc atccattttg catcttctgg cgttttgaca tccccgttcc
     3301 ccataaatgg aatcgtcaaa tgttttttca cttctttcaa tacttcccag tccgcttttc
     3361 cttcatacat ctgaacccgt gtacgtccat gcatagcgat ggcacttgct ccagctgatt
     3421 gtgctgctaa tgcattttct acagcaaaga cgtgctcttc atcccaacca atacgcatct
     3481 tgattgttac tgggatattt actgctgaag aaacagcttg gaccatttcg tatactttct
     3541 caggatctaa aagccatttt gctcctgctt ctgctttgat tactttattt actggacagc
     3601 ccatattgat atctattata tcggcagctg tgttttcttc gacaaattga gcagcttcaa
     3661 ctagactatc tttatttccg ccaaatatct gtaaacttaa tggatgttct gtttcttcaa
     3721 tgtggagcat ctctaaagtt tttttgtttc tgaaatgaat accttggtca ctgatcattt
     3781 cacatacgac taagcccgca ccaaattctt tgacagtgac tcgaaatgct gaatttgtta
     3841 ttccagccat cggtgcaaca acgacacgat tagggatttc cacattgcca attttccatg
     3901 tgtgttccac ttaaatccct cctaaggctt ctttcttcaa tccatctaat tcttcttcgc
     3961 tatacttgta tttgttatta cagaaagaac aaaccgcttc tgcaccgtga tcttcgtcta
     4021 tcattgcttg gatttcttct gcacctaacg cgataattgc ttctgcaaat ttttcttttg
     4081 aacagtcaca tttgaattga acaggcattt tttccaaaat gttcaattct tctgtaccaa
     4141 gcaatccttt caatatttct tctggtgtct gtccttcatc taagagggta gagattcttg
     4201 gtgtcgtttt taattgttct tcgatcttgc tgattgtttc ttcatcggca cctggcatga
     4261 tttggatcat gaatcctccc gccgtcttga tgctgtcgtc agtattgacc aatacactca
     4321 gtccaacagc tgaagggatt tgttctgaga tagcaagata gtaagtaaaa tcttcagcga
     4381 tttctccaga aacaatcggc gtttgcccag aaaaaggttc ttttaatcct aagtcttttg
     4441 tgacagtaaa cattccttgt gttccaactg ctccccgcac atcgatttta ccttcactat
     4501 ttagaggaag actgagatgc ggatttttga tgtaaccttt cacatctccc tttccatttg
     4561 catctactac aattcctgtt gctggaccat cgccttggat tcgaacagtt agtttgtctt
     4621 ctcctttgag cgtcgcacca agtagcagac taccgaccaa tgtccgtccc aaagcagctg
     4681 aggaggtgtg ccaagtatca tgacgtcttt gtgcctccgc tacggtattt gttgcttgaa
     4741 cggcgtatgc tcggacgaat ccgtcgtgag caagtgcctt aaccaaataa tctttcattt
     4801 gttatgtact cctttttccg tttttaaact catagtgaat catactagca actactgctt
     4861 ttttcaactt tggaatgctt cttttaaaaa taattcagaa tcatctagaa aacaggacat
     4921 caccctattt acaacttaaa taaagaaatc tcccgaaaac cggtttttgt tccggttttc
     4981 gggagtcata tcagatcaat tgacctatat ttttttattt gtattgatcg tctgaatgat
     5041 tatcttctga ttgttgagaa tctgaatcat gttctgcttt attttcctca ttcagttcat
     5101 gttttgcttc ttcaaaatca cgtttttctt ccgcttgttt ttcagcgtct ttttcttcca
     5161 aagcacgttt tgcttcttca aaagttttcg cttctttttc gcttggataa tcatttgact
     5221 cagtaccttc aggcatcaca ccgtgttcaa acaatgactt gattgctttg gcatccaatg
     5281 tttcgaattc caaaagtttt tcagcaatca gtttatgctg tgcacgatga gcttcgatga
     5341 tttctcgagc tttatcatga gcttccatca agatttttcg tacttcttga tcaatctcga
     5401 aagcgacttg ttccgagtaa gcttttgttt gaccatagtc gcggccaaca aacacttgat
     5461 ggtttccttc atattgaaca ggacctaaac gatcactcat tccatattct gttaccatgc
     5521 ttcgtgctag agctgtcgct tgctcaaagt cattggaagc accagttgat tggacattga
     5581 agatgatttc ttcagctgta cgtccaccaa gtaagcctac gatttgttca aacatatctt
     5641 ctttcgtcat caagaactga tcttcttttg gtaaagcgat catatatccg ccggcgcgtc
     5701 cgcgagggat gatcgttact ttgtggacga cccgagcgcg actcaatact aatccgacaa
     5761 tcgtatgacc agcttcgtga taagcaacca tttcgcgttc ttttttgctg attacgcgat
     5821 cttttttagc tggacctgca atcacgcgat cttccgcttc atcgatatca gaagcatcga
     5881 ttttcttttt atttcttcta gcagctacta acgctgcttc attcaagaca ttttctaaat
     5941 ctgctccggc aaaacctggt gtctgttgag ccacaacttt caagtcaacg tcatctgcca
     6001 tcggtttatt ccgtgcatgg actcgcaaga ttgcttcacg accttttaca tctggacgtc
     6061 caactaaaat ctggcggtca aaacggcctg gacgtagtaa cgctgggtct agtacatcag
     6121 aacggttggt tgcagcaatg acgattactc cttcatttcc gtcgaagcca tccatttcta
     6181 caagcaattg gttcaatgtt tgttcgcgtt catcatgtcc gccgcccata ccggcaccac
     6241 gttgacgacc taccgcatca atttcatcaa tgaaaatgat agctggtgcg tttttcttcg
     6301 ctgtttcaaa caaatcacga acacgacttg ctccaacacc gacaaacatt tctacgaaat
     6361 cagaacccga gatcgagtaa aacggtactc cagcttctcc ggcaacagct tttgctaata
     6421 aggttttacc tgttcctgga ggtccttcta aaagtacacc tgccgggata cgggcaccta
     6481 attcaacaaa tcggcgagga tctttcagaa attcaacaac ttcaactagt tcttgttttt
     6541 cttcttccgc acccgctaca tcagaaaaac gtacgcgatt agcttttttg tctgcttctt
     6601 tggcttttga tttaccaaag ttcatgacac ggccgccacc gccaccgccg ccgccttgtt
     6661 gtcccatcat catgtaaaag aaaaagatga tgattacgat tggcaagaag ctaagcaata
     6721 gggaaaccca tataccgctg cttgattctt cttttactgt tgtagcaact ttgttttctt
     6781 ttgccagttc tgttacttgg ctcaatgttg tatcatttgg caaaatgatt gttgtgaaat
     6841 gggtagtagt cgtatctgtt gaacctaaga tagctaaccc accgctgtta gcgacggtct
     6901 gctgttcttt gtattcacca gtgatcttat atacaccatt tgcaggctgc acttcaaatt
     6961 ctttgatctt gcctgcttgt agttgttctg tgaattttga gtactcgata tctgacgatc
     7021 gctggccgtt attaccaaaa atgaaataaa cgaccattac catcgctaag attacgagga
     7081 cataatacaa ggcatttttc atcatgccgt tgttcttttt gttcatagtt gccctcccta
     7141 agcgtcaatt taatttaaca gttttttatc aatcctgacc attctattgt accataattt
     7201 aattgagata cagcattaat ttgattgata aacactaggt ttcaatacac cgatgtacgg
     7261 aaggttacga tatgcttctg cataatccaa gccataaccg accacaaatt catttggtac
     7321 gttgaagcca acataatctg cttcgatatt tactactctt ccttcaggtt tgtcaagtag
     7381 tgtaacaatt ttgactgatt ttgctttgcg gtatttgaaa agatccacta aataagcaag
     7441 tgttcttccg ctgtcaatga tatcttcaac gatcaacaga tcgcgacctt ctacattcgt
     7501 atctaagtct ttgacgattt ttacttcccc tgacgagact gttgcattcc cgtagcttga
     7561 aacatccatg aaatccaatt ccaaatacgt atcgatagaa cgaacgatat ctgccatgaa
     7621 tggtaccgct ccttttaaga cacctactac taggggattg gtgttttggt aaatttctgt
     7681 taactctttg cctagttctt tgcaacgaac ttgtatctct tcttgtgaga tcaaaattct
     7741 ctcaatatcc ttttccaaca tcaggttctc cttccaattg cttctttttg atatttgtaa
     7801 agaagtatgt agtgtatttt atcagtttct tcacgaatac tcaaatatga aaaggcaaaa
     7861 ggtatcaagc tatatatatt tcttttggca tctgtaatga tccaagcatg ctgcctttgt
     7921 gaattaggta ctttctgatc gataaaatat ctagatactt tttttgtgag attctctgtt
     7981 aatttaatgc ggtctcctgc ttttcttttt tcgacataca gttgcacatc agcaggaatc
     8041 caaatgtcat gagaaaatag agaccatgaa tctgttgctt tgatgtcccc tgcattttca
     8101 ggtttgacca gaccaatcca ttctgtttca ttcaaatata ttccttgttc tggaatcagc
     8161 aacaagcgct ggtcttctgt ctggctagtt gcttgttttt cttttttaga gagtacaaca
     8221 aaatgatact ctcttttcag tatccaatta ttgccaatac tgagttgcca ttggcttttt
     8281 ttctgttgcc actggtctaa aatcgactcc atttgttgtt ctttcaaaat aattcctgtt
     8341 tttgagaatg ctttgtcgaa aaaggagtaa aaaaaatagt atcgttcagc atctgtcatt
     8401 tcttcaatca tttctgcaga aaatcgaaat tgttcctccc actgttcgac ttccttttcg
     8461 atcaactgtc ccatatattt ttgaatgact tggtctgccc attggatctg ttgcgaaaat
     8521 tgctggaaat gtctcaacgc ctgtgtattt tcttgtttca attgaggaac taccaaatga
     8581 cgaagccgat ttctctggat atctaacaat tggtttgtct gatcctcaaa gtaaacaagc
     8641 tgcgattccg ctgcataagt atataggtct tctttgctaa aactcagcaa cggacgaatc
     8701 agtctgcccg tagcaaatgg ttgtacttct ttgattccag aaaaagtatt taactgtccc
     8761 ccacgaacga ttttcattaa caccgtctcg atttgatcat ctccgtgatg agcagtcatc
     8821 aacgtgtcat attgtccttc ttccatcacg cgagtgaatt cttgataacg aaagttacga
     8881 gcttctgttt ctatggcgga tttggctgga tcttcccaaa tacttacata taaagggaga
     8941 ttcttctctt cacaatatga gcgaagatag gcagcttctt ctgctgattc ttctcttaat
     9001 tggtgattga tgtggacgat acctaaagaa aaaccgattt ggcgttgcac ttgtaaaagt
     9061 aaatggagca aaaccatcga atcaacacca cctgataccg ctgctaaaat tctcgatcct
     9121 tgttgccaga atccttgttc attcccgtgc cgaataaatg actgctgtaa tagcggtgct
     9181 tttttcccgc ctttttcaac gttcatcctt tccacaccct ttcttcctaa aaaaaagaga
     9241 gcgggaaaac gtctaaaacg acaattaacc tgccctctgt tgatactatg acccaaatag
     9301 tcgcactatc attatttgcg gctttttagc tgcgacgtcc gccacggcca ccgcgctttc
     9361 cttctgtgtt tcgcttcaat gaagataagc ggtcatcgct atctttcaaa aatgaactca
     9421 tcaatgaatc gaagtcttct ttttgagctg aaggttgact gcgagaaaaa gattttttct
     9481 gtgctggacg atttcggttg tcacggggat ctttttgttg atatttttta ggacggcttt
     9541 cttctccaga ttcagcaggt tgttcctgcg ctttccggat agatagtccg atttttccat
     9601 catcatttac tgaaataact ttaactgtta cttcatctcc tacacttaaa acatcatgga
     9661 tgtcttttac atagccattt gacacttcgc taatatgaac tagacccgtt tttccagcac
     9721 ctagatcaat aaacgcacca aaatttgtga ttcctgatac ttttccttgc agttttgctc
     9781 ctacctcgat tgacataaaa aaaatgttcc tcctaattat ttcccttttt tatattcctg
     9841 cttattttgc agttgtagaa ctagttgatt gatctgtttc atttgtcgag ctgtctgtat
     9901 ttgattctaa tataggataa actttttcac cgtcttttga gtaaaagaat ttgcttctgg
     9961 caagtttagc tacatactca tcatctttca gcaatgaaac atcttggtgc agctgatcaa
    10021 cgtctgcgtt gacttctttc atttcagtat ttgcttcgac tctcaattcg ttcaattgat
    10081 gcatcctttg catgtcatgg aaaatctgca cacctattat cgcgaaaatg accaaagcac
    10141 caacaaaaat tgcagccagt ctgcgtcttc gaaaaatcag ttgacgttgt tgttgctgaa
    10201 attcagcata ctttttcttt gcatattccg tatctagcgc agcgatttta ttgctttttt
    10261 tcgtcacttg gattcgctcc aatctttttg attgtctttt tcattatacc aatatcgtga
    10321 aagcttgtct aatctatttc tctagtgttt ctgtgcgatt ttcagaaata atttcgtaca
    10381 ttttttgtgc atcttctttt ttggttgatt catgtaattc cgtaattttc acttccaacg
    10441 ttttgttgcc aaactggact ttcaaaatat cgcctacttt gacatcactg gaagatttcg
    10501 ccaatttacc gttgatttga attcttcctt tatcagctac ttcttttgct actgatcgtc
    10561 ttttgatgat tcgggaaatt tttaaaaatt tatctaatcg catatcttat tcctcacttt
    10621 ttaatttttt tatttaatcg caggattttc ttaccaaaag gcaacattag ccattctcgt
    10681 actgtaaaca gacgaagacg aatcactgca taaatgaaca cagaagcccc gatcccaact
    10741 cctatcacgc tgacaaaaaa agcagtggaa cgatgagtta caggaccaaa tactatattc
    10801 aatacaccat aatacagcag cagtactact gtcatacaaa gaaggctgaa catcaacttc
    10861 cgtccaaaat gccgttcttt ccaaaaagca tttagctctt ttgtttcaat tcgaacaaaa
    10921 taccaaagcg tcacacctaa tcctaaaagg gtagacaggc ttgcacctgc tgttcctaat
    10981 aaaccggtca gaaaagaagt agtcaatcct tttaccaata gtccccatcc tgcgcctttt
    11041 aaagaagggc gaaacgagtt cttgctttga gcaatacttt gataagcttg gatgactgca
    11101 gtgaaagcaa tggaaaaaac aaataataca agcgctgcat ttcccgcata atctttaaac
    11161 aacgcataat tgatataagg gagcagcaag gctaatccca aagacgcagc taatgctaaa
    11221 gcggtcgtca atctaagata gattttggct gtctgtaaaa actgacgata gatccggttg
    11281 gtcaaatgac gagtcaacgc aggcaggaat gtcgcactta atgcagtggc aatcactaaa
    11341 cctaattgta ctaaaggctg acctcgatca tagacccctt ttgctatctt cgcaccgtat
    11401 tctgacagtc cgtaaacttc caaagcattt tttacaaaga aagagtcgat cagctggaaa
    11461 aagataagca atccactata aatcgaaacc agtccgcctt ctaccagaaa acgccggatc
    11521 aagggctttg acggctgttt gaacaatgga aaccgtcgga agcttaacgc tcctccatga
    11581 atcttttgtt catagtagtg aaggatccca tatgcgcata aaccgccaaa aacagctcca
    11641 ctcattgctg ccgtccctgt ttggtacacc gtccatccaa gctttctaaa agcaaacgca
    11701 ctacagacaa tcacaaaaac acgaacgaac tgctcaacta cttgagaaac agcagttggt
    11761 tccatcaaga atcttccttg gaaattccct cgataaaaag atagaccagg catcagcaaa
    11821 aaggtaaagg aaactacttg gatcagcggt ttcaattgta tgtctcccat catccaagca
    11881 atccagctgc taaatgcaaa gacaaacaac cacaaaaaga cacctgtcca aaatactaag
    11941 ggataaagat tttgcaaagc ttgctttcgc tcgaaaggat tctttctttc tgcaacgtac
    12001 tttgatatga actgcggaag accggataaa gctaaagtca ttgctatccc ataaattgga
    12061 taaacttgct gataaacata aaaaccttcg tcgccgacta agttttggag gggaattcgg
    12121 taaagggcac ttaatacttt tgctatgaaa gaagcaatcg tcaatacaaa tgctccttgc
    12181 atgacgcgcc gcatttcttt atgatccaaa tcattgctcc ttcctacgct gacagatatt
    12241 tttgctcacg caacgctttg acaaactttt ggatttcctg aagccatact gcttcttcca
    12301 tttggttggg gatcgtcaag cgtatcacca tctgttcttt ttcaacgcct agactggctt
    12361 ttagttttgt cgctgtcagt gcttcgaata actgttccac actatagcgt tttgttccaa
    12421 ctttacttag agtaaagact atatgcgcct cctgtttcct tatcgtttct aggagcgctc
    12481 gatcgccatc cattttgatc tgcccaattg tcaagagatg tgctacttct tctggatatt
    12541 ctccaaaacg gtcgagcaga tcatcttcta gttcatctag catttcttgc gaatcaagtt
    12601 cacgaattcg tttatagatc tcgattttct gccgttgatc tgccacgtat gtttcaggta
    12661 gatacgcatc gacaccgagg tcgatctcaa cagtggtctt ctccacttga ctattctttc
    12721 cttgtttacg agaaacagct tcgcttagca tttgagaata catatcgaaa ccgactgcat
    12781 caataaatcc atgctgctga gcacctaaga gattacctgc accgcgaatg gaaagatccc
    12841 gcatcgcaat cttgaatcca gatcccagtt ctgtaaaatc tttgattgcc tgaagacgtt
    12901 tttcactgac ttcgttaagg atcttctgct gctcatacat aaagtaagca tatgcgacac
    12961 ggctgcttct tcctacccgc cctcgcaatt ggtataacgt ggataatccc atataatcag
    13021 cgttctcaac gaacaacgta tttgcgttcg ggatatctac tcctgtttca atgatggttg
    13081 tagtgacaag aacatcatat tgaccttcaa taaattcaaa aagcgtattt tctaattgcg
    13141 cctctgtcat ttgcccatga gcataaccga tccgtgcttc cggaacgagt tcttggattt
    13201 cttctacttt ttgttcgatc gtttctaccc gattatataa atagaatact tgtccacctc
    13261 gtcccatttc tcgatggatt gcttcgcgaa tagccccagg atttttttcc ataacatacg
    13321 tttggatcgg ataacgattc gcaggcggtg tttcgattac tgaaagatcc cgcacgccta
    13381 acatcgacat atgcaaagtt ctcggaatcg gtgttgcagt cagagtcaaa acatccactt
    13441 gtgcacgtaa ctgtttcaat cgttccttat gttttacacc aaaacgttgt tcttcgtcaa
    13501 tgatcagtag acccaaatcg ctaaattcaa tatcttttga caaaattcgg tgggttccta
    13561 caacaatatc aacttgacct gtacgcagtt gttcgatggt ttctttttgt tgctttcttg
    13621 ttctgaaacg acttaatagc ccgatattta cggggaaacc ttcaaatctt tccagcatcg
    13681 tttcatagtg ctgctgtgcc aagatcgttg ttggaactaa aaaggctact tgtttgcttt
    13741 cctttatcgc tttaaaggcc gctcgaagcg ctacttctgt tttcccataa ccaacatcac
    13801 ctacgagtag tcgatccatt ggtttttctt tttccatatc ccgtttgatc tccgctgcac
    13861 ttctcaactg gtcatcagtt tcagagtagg ggaaggcatt ttcgaattct ttttggtagg
    13921 cgtcatcagg tccaaaagca tatccttttt ctgactctct tgcggcatat aatttgatta
    13981 gatcgtcagc gatatcctcg atttttgaag agacttttcg cttggttttc gtccattcac
    14041 ttccgcctag cttattgatg cgaggcgctt tggcttcaga ggcgacatat ttttggatca
    14101 gattcagttg agtaacagga ataaacagtt tgtcgtcgtt ttgatacaaa atagtcatat
    14161 agtcttggtg aacgccatct acttctagcg tttccatccc aatgtatttc ccgataccat
    14221 gattggcatg gacaacgtaa tctcctgctt ttaactcatt atagcttttc aatcgctctg
    14281 cgtttgttac tgtttgacgt ctcgtttgtt ttttcgtcgt tttttggaaa atttcttttt
    14341 ctgtgatagc tactattttt tcttgaggaa gttcaaaacc agcctgcaaa gctccttcaa
    14401 ctaattgtac ctgtcctctg atcaatgcat ttttttctgt ttctactact aatatgtctt
    14461 catctcgaag catttgttct gctttgcgta ttctgtcctt tgtcggaata aagatcacaa
    14521 cagtctgttt ttgcttgcgc caacggtcca tttctgtttt cagcaatggc atttgtccga
    14581 agaactgttg catcgtccga tattgaaaat tatggattgc ttggaaacga agatttccca
    14641 tccctttttg gaagagtgag aaaaaggttg tggtgaactt ttccttctgc acgatttttt
    14701 gtatatctgc tgaaaatgtc tgctcagaaa acacccgcaa ttcgctgatt ttttgagtct
    14761 gccattctgc cgcttccctt tcgatttccc gatttgtttc catgattcgc tgataatcat
    14821 caacgactag taaactattt gaagggaaat aatccaagat cgtcacagat tcttggtaaa
    14881 gaagatctga gtagaatttt gcttcttctg tcgggacacc tgcttgccag cttgaagtca
    14941 gctgaccgaa gtaatcttgc aaaaactcgc ggtctgcttc ttctgtggtc gcagcaagcc
    15001 gtttttctag cattttttgc atttgctcta ttccatgttc tagatcctct ggtccgtaga
    15061 cttgttcact cgttggggaa acccagattt cttccgtttt ttcgattgag cgttgtgttt
    15121 ctgcttcaaa ataccgcata gaatctatct ctacatcaaa tagttctaca cgaacaggat
    15181 aggttgtcgt taacgggtaa atatcgataa tgcttcctcg aatactgaat tctcccggtt
    15241 ttgcgaccat ctcttctcgt acatagccca tcaaagtcaa ttggcgagga agattttcca
    15301 cgtctatctc atcaccaata tgccaattca attgcgcctg tagccaagtt ttcttattcg
    15361 gcagtcgttt gtgcaaagat gcgactggca acaaatagat acctgctttt ttttcttgaa
    15421 gtgcgttaag cgttgcaact ctctcgcctc gcgcttctgg agaagaaaag gccatttcag
    15481 cagaaagcac ttcatccact ggaaataaat ggatctgatc ttgcggaatc acatgttgca
    15541 gatcttctgc taactgattc gcataataca aatttggcat cagaataatc atgtttttat
    15601 ttgttttctt aaacgctcga gcaaatagaa gtgttttcgc tgagcccgct aatcctgtga
    15661 ttagttgtct aggcattttt tcttctaaat tgctgaccca ttcttttact tgcggatctt
    15721 ttccgactaa ttgaataata tccattcctt ttcctccttc ctcaattgaa tcgattcatc
    15781 gcatccacga acgaatgccc ttctatgaga tattcgactg ctttaacaga ttgacacatg
    15841 ctttcttcta tcaaaggctg attttctttg ctgaaaggac taagcacatg ctggactact
    15901 gtttttttcc cttctggcct gccgattccc accttgattc gatcaaagac attcgtattc
    15961 aaatgggaaa taatactttt gataccgtta tgcccgccgg cacttccctt ttgacggagc
    16021 cggatttttc ccactgccag atccaaatca tcgtagatta cgactaattc ctctggataa
    16081 atcccaaaat aagtcattag tgggccgact gcacgaccag attcattcat gaatgtttgt
    16141 ggtttgacta aaaggatctt ttctccatta tgaaaaaatt cggcaacttc tgcttcgaac
    16201 ggattttttt tgaaagtagc ttgatgctcg cgtgcaattt tatccacgac cataaagccg
    16261 atattgtgtt ttgtatattg gtatttggta cctgggtttc ctaacccgac gatcattttc
    16321 atttttgttt tccacctaat tctaaaaagc agcacaaaag gctggactgc taattagcgg
    16381 tccagtccat cgagcttttt tcttttttac acttgtcaat gcatagtata acacaaaaca
    16441 aggcgtttcg gctaaaagag aatcgttaat aaatgataaa gaaattaaaa atttcttcaa
    16501 agtttaatga aataattgat atataaaaat agtgaaataa cgcacatgta agcttttaca
    16561 tataatacaa ccttgttatc tgttatattt tatagccaaa tagcgtgaca tggggcttga
    16621 gaaatgttag cataagcatg ggaaaaaaca aatcaatgga aggattgata ctaaatgact
    16681 gcagctgcag aaaaaaaaga tcatcaaaaa gttattcttg ttggagacgg cgccgtaggt
    16741 tctagctatg cctttgctct agttactcaa aatatcgctc aagaagtagg tattgtggat
    16801 atcaatactg ctaaaactga aggagacgca attgacttgt ctcacgcttt ggcatttact
    16861 tcacctaaaa aaatctattc tgctacttac gcagacgcac atgatgctga cttagttgtt
    16921 attacagctg gcgcaccaca aaaaccaggt gaaactcgtt tagacttagt aaacaaaaac
    16981 ctaaaaatca accgcgatgt tgtcacacaa atcgttgaat caggatttaa cggtatcttt
    17041 ttagttgccg ctaacccagt agatattttg acttactcaa cttggaaatt ctcaggtttc
    17101 cctaaagaac gcgtaatcgg ttcaggtacc tctcttgact cagctcgttt ccgtcaagca
    17161 cttgcggaat tagttgacgt tgatgcacgt aacgtccatg cttacatctt aggtgaacac
    17221 ggagattcag aattcccagt ttggtcacat gcaaatgttg ccggcttgca aatctatgaa
    17281 tgggtaaaaa acaacccaga aatcgatgaa gaagctatgg ttaacctatt ctttggcgta
    17341 cgtgacgctg cttacacaat tatcgagaaa aaaggcgcta ctttctatgg tatcgcagta
    17401 gcattagcac gtatcactcg tgccatctta gatgatgaaa atgctgttct tcctttatca
    17461 gtattcatga atggcgaata tggattgaac gacatctaca ttggtgcacc tgctgttatc
    17521 aaccgtcaag gtatccaaaa agtaatcgaa attcctttaa gtgacagcga gcaagaccgt
    17581 atggctgctt ctgctaagca attaaaagaa attctagatg aagcttttgc taaactagac
    17641 gctgaataat ctttgaacat gacggaaaac atctattcgg tgttttccgt ttttttattt
    17701 tatcaccctt atggtttcta aattttttca taaagtagag ataaccttcg tcttttcctc
    17761 taaattatgc tatcatgaga ccgttccacc ctaaactttt acttaaggag ttgtcgatat
    17821 gacaagatct tcgcatcgaa aaaaatcgaa acatgttgct ttgctttcag ttcttggtgt
    17881 tctcttagtg ctgatcagct tttataccta tcgcagtaca tactacacaa agcatttttt
    17941 gccgaataca gaaataaatg gaataaatgt cagcaatcta acagtcgaaa aagcaaatga
    18001 aaaattaaaa gaagcttatt ctgataaaaa actggctatt aaagaagatg gaaaagtctg
    18061 gcaggaagta ccaaaatccg agctcggcta caaagatgat ttcacttcgg agctttctcc
    18121 gatcctaaat gaacaaaacg gatggacttg gggaatgact tatgtctcag cggcagaaaa
    18181 acaagaaata gatcctgtca gtcaaaacca ccaaaaacta gacactaccg ttcagacatt
    18241 gacaactaag ctgactgatt tgaacaaaga ccggacacct actactgatg caacgatcga
    18301 aaaagcaggt gactctttcg ttatcaagcc tgaagtcaat ggaaatacga tcgacgtcga
    18361 tgctgcggtc aaacaattaa aaagtgcggt aaactctggt aaagatacaa ttgaattgac
    18421 tgaattcaaa gaaaaaccaa aagtaacatc tgaagacagc tcactgaaag aacaactcgc
    18481 atccatgaat gctatcgcaa atgtaaaagc tacttacagt ataaacggtg agactttcca
    18541 gatcccatca agtgatatta tgagttggct gacctacaat gatggaaaag tcgatttaga
    18601 tacagaacaa gtacgtcaat atgtaacaga tttaggcaca aagtataaca caagtacgaa
    18661 tgacacaaaa tttaaaagta caaaaagagg agaagttacg gtcccagttg gcacttacag
    18721 ttggacgatc caaacagaca gtgagacaga agcattgaaa aaagcgattc tggcaggcca
    18781 agattttact cgttcaccca ttgtgcaagg tggcacaacc gctgatcatc cattgatcga
    18841 agatacgtat atcgaagttg atctggagaa ccagcatatg tggtattaca aagacggaaa
    18901 agtggccctt gaaacagata tcgtttccgg aaagccaact acacctacac ctgctggagt
    18961 cttctatgtt tggaataaag aagaagatgc aactttaaaa ggaacaaatg acgatggaac
    19021 accttacgag agtccggtaa actactggat gccgattgac tggacaggtg ttggtatcca
    19081 cgattcagac tggcaacctg aatacggtgg agacttatgg aaaacccgcg gttcacacgg
    19141 ctgcatcaac accccaccaa gtgtaatgaa ggaattattc gggatggtcg aaaaaggaac
    19201 gcctgtattg gtcttctaac aaaaataata tataaaaaga aagcacccgt acgattggta
    19261 caggtgcttt ctttttatta tctaacaatg tgctacttac atttcagcag cagtaaagac
    19321 ttctgaaaca tcatcatcgt cttctagctt atctactaga cgttccaatt gctctttttg
    19381 ttcctcattt aaatcaatcg tcgtttgtgg aatcatcgtc aattctgctt gcgccaaagt
    19441 gaactctttt tccaactcat ctcgtacagc agcaaaatct tctggtgcag tataaatctc
    19501 gaatacttca ggagaagttt gcagatcttc tgcccccgcc tctaatacat cctcaaacat
    19561 cgtctcttca tcaacagtca acccatcgcg ttcaatagcg atatatcctt tgcggtcaaa
    19621 caagtaactg actgaacccg tttctcccag tgatccaccg tttcgagtaa aagctactcg
    19681 gacatttgtc gctgtccgat tacggttatc tgtcaatgca tggactaaga ttcctactcc
    19741 acccggacca tatccttcat acgtgatttc atcgtaattc gtttcatcta cggcactcgt
    19801 agccttttta atcgcccgtt ccacattgtc gttaggcata tttgcagctt ttgctttgtc
    19861 cacaacaagc cgcaaagctg gattcataga tggatcagga ccgcctgctt tagctgccat
    19921 ataaatttca cgagaaagtt tctgaaagac tttccctcgt tttgcatctt gggcattttt
    19981 tcttccttgg atattatgcc atttactatg tcctgacatg atttttcctc ccttgttctt
    20041 ttgagaatac acttctattc tagtactatt tcacaaaaaa acaaagccac agccaatcta
    20101 taaacgcttc ttgaatgccg caaaaagcac acataatatc acagcagtca gagcaccgat
    20161 cgaatccaac atgacatctt ggaacatagg tgttcgtcca ccggtcatca tctgatggaa
    20221 ttcgtccaaa cctgcgtacc ctgttgctga aagccaagca aggatcgctg tcagccacca
    20281 aatttttagc ttggggaaaa cacctaaaaa caagctgcct cccaaaataa aataggtacc
    20341 gaaatgtgcg cccttcctta aaaagaactc taaaaatttg gcgtagccca gatggttgat
    20401 actcaccata cttccaccat aattaaaacg tatacccgct aatgtgtctt taaatggctg
    20461 attaggcagc cagctttcta atcgcgaaac ttgtgattgc tgtccgtaag tttgagaaga
    20521 actgtaaaat aagattataa cgactatcag tgcgattact aaataaatat ttccgttttt
    20581 catttgtttt tttgaagaat tcataagtca cctctgtatt ttatcttaga ctttttttga
    20641 agaaacaaca atcattttca tagagacttc ttccataaaa aagtgctata cttaattttg
    20701 tattacaata tgaagggagt tttttcatga gttattcatt aaattggaat ctagattcta
    20761 ttttttccgg aggcagtcat tctgatgctt tgaaccaacg gatgaagcaa ttggaagacc
    20821 aaatgaacga gtattatcac cgcgtaacaa aatggtcacc tagttctgac aatgcagagc
    20881 agttaaacgc tattcttcaa ttgcaagaaa cgattacgaa tggttttacc caatgcagca
    20941 gctatattac tgcattgctt tctgccaatg tcaatgattc agatgcaaag gtcttatcag
    21001 gcaagcttta cgcaatgctg cctcgtctgc aatcagccga aactgtattg tcaaagaaat
    21061 ttgcagaaat atctgacaat gattggaatc agatgctttc tcaatcttcg tttgaaacaa
    21121 ttgcttttcg tttaaacgag attcgtcgtg atggctcaca attattatca gaagctgaag
    21181 aaaacattat aaatacgctt tctttagatg gtctaaatgc ttggagcagt cattatgata
    21241 cgattgtagc cagcatttcg attcccttcg aacaagatgg acaagtagtc gaattatctg
    21301 ctggacaagc cttcaataag atgatgggcg atccagatcc aaaagtcaga gaaacacttt
    21361 tcgcagcatg ggaagaagcc tggaaagaaa aggcacccct cttcacggat actttgaatc
    21421 atttagatgg ttttagactt tctgactatg aacttcatgg tgtcacagat tttctacaaa
    21481 aaccattaga gtacaatcgg ttgaagaaag aaacattgtc tgtcatgtgg gatacaatcc
    21541 agaaaaacaa acaacccctt gttaattact tgactagaaa agctaatctt ttcggtaaag
    21601 aaaaaatgga atggcaagat caagatgcac ctattatttt aggcgatttg aaagaaaaaa
    21661 cctttagttt tgacgaagca gcagcattta ttatcgaaaa ctttaacaaa ttcagtccaa
    21721 aaatggctaa atttgcccaa tctgcttttg aaaagagctg gatcgaagca gaagatcgtc
    21781 ctgggaaacg tcctggtggt tattgtactg agttaccaga aacaaaagaa tcaaggatct
    21841 ttatgaccta cagtaattct gttaatgagg ttgcgacact tgctcatgaa ctaggacatg
    21901 ctttccatag cagcacgatg tgggatttac cttctttaaa tcgcgaatat gccatgaatg
    21961 tcgcagaaac agcaagtaca ttcgcagaac tgatcgtagc cgatgctaca ttaaaagtcg
    22021 caaaaacaaa agaagaaaaa atcaatttgc tagatacaaa actgcaaaat gctttagcta
    22081 tgttcatgaa tatccattcc cgattcattt ttgaaaatcg attctatgaa gcacggcaag
    22141 aaggattagt ctcagaagat aagatcacag agatgatggt tgaggcgcaa aaagaaggct
    22201 atcaaggagc tttagctact taccatccat atttctgggc tgcaaaattg catttcttta
    22261 ttgatgacgt accgttttat aacttcccat acacatttgg ctacctcttt agtttgggca
    22321 tctatgctta cgccaaccaa aagggagcaa attttgaaga agaatatatc catttactac
    22381 gcgatactgc ctcaatgacg acggaagatc ttgcgaagaa acatttgaat gtcgatctta
    22441 cccaaccaga tttttggcaa gcaggtatcg acatggtgac tgaagatatc caaacgttca
    22501 tggaattgac agaagaattt atctgatttc tattttatca caataaaaaa agagttagtc
    22561 cagaatgggc taactctttt tgattaatca ttcttacatg ctgacagcaa agtcaggggc
    22621 aaaccattga actgaaccaa cttttacgct ttcgcctggt tgaggagcat ggatatattg
    22681 tcctccgcct aaagcaatcg ctacgtggta agttccgcct tgtgaccccc agaataacaa
    22741 gtctccagct tttgcttgag aaactgaaat ttttgttcca gctgattctt gaggaactgt
    22801 ccaaccaccg atgtcacgac cagttacttg catgtaaaca tagcgtgtga accctgagca
    22861 gtcaaagcca cttggatctt taccgcccca aacatatgga gtaccaatat atttgtaagc
    22921 ttctgctacg attgctgcac cattcacact tccagaagga gctggagcag gtgttacagg
    22981 ttttggttgt tcaggtgttg gtgttggcgt tggagccgga gtactacttc ctgtgccgtt
    23041 tcctgtatca acactttgat ctgttgatgg tgttgtaggc gcaggtgttg ttgattcttc
    23101 tgttg
//
LOCUS       DAYYBG010000041        22788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000041 DAYYBG010000000
VERSION     DAYYBG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.041"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACHAUN_001503"
     CDS             complement(11..1942)
                     /locus_tag="ACHAUN_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8460207.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACHAUN_001504"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACHAUN_001504"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8460208.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACHAUN_001505"
     CDS             complement(2871..3623)
                     /locus_tag="ACHAUN_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8460209.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACHAUN_001506"
     CDS             complement(3782..3991)
                     /locus_tag="ACHAUN_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460210.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACHAUN_001507"
     CDS             complement(4002..5330)
                     /locus_tag="ACHAUN_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460211.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACHAUN_001508"
     CDS             5466..5621
                     /locus_tag="ACHAUN_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8460212.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACHAUN_001509"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACHAUN_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8460213.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACHAUN_001510"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACHAUN_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8460214.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACHAUN_001511"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACHAUN_001511"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8460215.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACHAUN_001512"
     CDS             complement(9305..10237)
                     /locus_tag="ACHAUN_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460216.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACHAUN_001513"
     CDS             complement(10394..11053)
                     /locus_tag="ACHAUN_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8460217.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACHAUN_001514"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACHAUN_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8460218.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACHAUN_001515"
     CDS             complement(12094..12384)
                     /locus_tag="ACHAUN_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460219.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACHAUN_001516"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACHAUN_001516"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8460220.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACHAUN_001517"
     CDS             complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACHAUN_001517"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8460221.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="ACHAUN_001518"
     CDS             complement(16049..17218)
                     /locus_tag="ACHAUN_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8460222.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACHAUN_001519"
     CDS             complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACHAUN_001519"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8460223.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACHAUN_001520"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACHAUN_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8460224.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="ACHAUN_001521"
     CDS             22182..22703
                     /locus_tag="ACHAUN_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8460225.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6353 a         4948 c         4055 g         7432 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata aggagcgtgg gacaaaagta aagaacactt
    22741 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactc
//
LOCUS       DAYYBG010000042        22383 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000042 DAYYBG010000000
VERSION     DAYYBG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.042"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(237..689)
                     /locus_tag="ACHAUN_001522"
     CDS             complement(237..689)
                     /locus_tag="ACHAUN_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8460226.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(702..1112)
                     /locus_tag="ACHAUN_001523"
     CDS             complement(702..1112)
                     /locus_tag="ACHAUN_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8460227.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1387..1599
                     /locus_tag="ACHAUN_001524"
     CDS             1387..1599
                     /locus_tag="ACHAUN_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8460228.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1602..1814
                     /locus_tag="ACHAUN_001525"
     CDS             1602..1814
                     /locus_tag="ACHAUN_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460229.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1954..2109
                     /locus_tag="ACHAUN_001526"
     CDS             1954..2109
                     /locus_tag="ACHAUN_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460230.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2106..2330)
                     /locus_tag="ACHAUN_001527"
     CDS             complement(2106..2330)
                     /locus_tag="ACHAUN_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8460231.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2408..2596
                     /locus_tag="ACHAUN_001528"
     CDS             2408..2596
                     /locus_tag="ACHAUN_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460232.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2593..2766
                     /locus_tag="ACHAUN_001529"
     CDS             2593..2766
                     /locus_tag="ACHAUN_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460233.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2735..2863
                     /locus_tag="ACHAUN_001530"
     CDS             2735..2863
                     /locus_tag="ACHAUN_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460234.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2922..3257
                     /locus_tag="ACHAUN_001531"
     CDS             2922..3257
                     /locus_tag="ACHAUN_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460235.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3490..4431
                     /locus_tag="ACHAUN_001532"
     CDS             3490..4431
                     /locus_tag="ACHAUN_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8460236.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4433..5323
                     /locus_tag="ACHAUN_001533"
     CDS             4433..5323
                     /locus_tag="ACHAUN_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8460237.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5366..6166
                     /locus_tag="ACHAUN_001534"
     CDS             5366..6166
                     /locus_tag="ACHAUN_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8460238.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6181..7032
                     /locus_tag="ACHAUN_001535"
     CDS             6181..7032
                     /locus_tag="ACHAUN_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8460239.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7098..7232
                     /locus_tag="ACHAUN_001536"
     CDS             7098..7232
                     /locus_tag="ACHAUN_001536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460240.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            7233..7694
                     /locus_tag="ACHAUN_001537"
     CDS             7233..7694
                     /locus_tag="ACHAUN_001537"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8460241.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7720..8172
                     /locus_tag="ACHAUN_001538"
     CDS             7720..8172
                     /locus_tag="ACHAUN_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460242.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8278..8472
                     /locus_tag="ACHAUN_001539"
     CDS             8278..8472
                     /locus_tag="ACHAUN_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460243.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8503..8796
                     /locus_tag="ACHAUN_001540"
     CDS             8503..8796
                     /locus_tag="ACHAUN_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460244.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8727..9266
                     /locus_tag="ACHAUN_001541"
     CDS             8727..9266
                     /locus_tag="ACHAUN_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8460245.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9341..9808
                     /locus_tag="ACHAUN_001542"
     CDS             9341..9808
                     /locus_tag="ACHAUN_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFM8460246.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10555..10785
                     /locus_tag="ACHAUN_001543"
     CDS             10555..10785
                     /locus_tag="ACHAUN_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460247.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10944..11777
                     /gene="terS"
                     /locus_tag="ACHAUN_001544"
     CDS             10944..11777
                     /gene="terS"
                     /locus_tag="ACHAUN_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8460248.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11770..13185
                     /locus_tag="ACHAUN_001545"
     CDS             11770..13185
                     /locus_tag="ACHAUN_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8460249.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13197..14726
                     /locus_tag="ACHAUN_001546"
     CDS             13197..14726
                     /locus_tag="ACHAUN_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8460250.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14644..15696
                     /locus_tag="ACHAUN_001547"
     CDS             14644..15696
                     /locus_tag="ACHAUN_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8460251.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15698..16015
                     /locus_tag="ACHAUN_001548"
     CDS             15698..16015
                     /locus_tag="ACHAUN_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460252.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16142..16765
                     /locus_tag="ACHAUN_001549"
     CDS             16142..16765
                     /locus_tag="ACHAUN_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8460253.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16780..17670
                     /locus_tag="ACHAUN_001550"
     CDS             16780..17670
                     /locus_tag="ACHAUN_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8460254.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17693..17908
                     /locus_tag="ACHAUN_001551"
     CDS             17693..17908
                     /locus_tag="ACHAUN_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460255.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17920..18261
                     /locus_tag="ACHAUN_001552"
     CDS             17920..18261
                     /locus_tag="ACHAUN_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460256.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18261..18632
                     /locus_tag="ACHAUN_001553"
     CDS             18261..18632
                     /locus_tag="ACHAUN_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460257.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18625..19020
                     /locus_tag="ACHAUN_001554"
     CDS             18625..19020
                     /locus_tag="ACHAUN_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8460258.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19022..19399
                     /locus_tag="ACHAUN_001555"
     CDS             19022..19399
                     /locus_tag="ACHAUN_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8460259.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19400..20008
                     /locus_tag="ACHAUN_001556"
     CDS             19400..20008
                     /locus_tag="ACHAUN_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8460260.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20008..20349
                     /locus_tag="ACHAUN_001557"
     CDS             20008..20349
                     /locus_tag="ACHAUN_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460261.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20591..>22383
                     /locus_tag="ACHAUN_001558"
     CDS             20591..>22383
                     /locus_tag="ACHAUN_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8460262.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKE"
BASE COUNT         8031 a         3293 c         4595 g         6464 t
ORIGIN      
        1 aatataaatc tttctctatc atgtaattta tttagttttt catcattttt ttgttctaat
       61 tcataagctt ctttttttat tgtgttagct ttcgacagtt tagtgattcc tataattaat
      121 aaaataagtc caatcaataa taacattcaa aactcctcat ttctttgata taattattgt
      181 agagtttact cagaaatgag gaaagagtcc atgtgaaagc atgggctttt ttatttttaa
      241 tgttgtaata ctaaatgttc aagattatga ggaagcccta aatattcaat aatttggaac
      301 tgactcatat cagataaatc ttgttcatct attgttctag ttaacatttc aattgcaaac
      361 atattagctt cagcttctat accataaatc atacaactaa ggttgtttgc tctaaaagct
      421 gctgtgcaaa tacctttatg gagtctaacg tgggctattt catgccataa tacaaatctt
      481 ttcatagaag gtgataggtt ttgattaatt aatatcacat aacatctttt attgtgtatc
      541 gtttgtccta atgttttatt ccctaaatca gtttcaatta aatctatatc agaatatttg
      601 attattttat atggattaat cgcatcagat ccaaactcta caaataattt ttctatttct
      661 tctaatatcc agtatttgtt taaatgcata ccaaagccca cctattgacg atatttttta
      721 ggagtgaatt ttttctttgc tttaactttg gttaaggcaa gtgcaagttg aatggcatcg
      781 tataattttt ccttatcttc atcgtctaaa ggttcaccat aataatttgt ttcagcatct
      841 gaattcaagc cttcaagtaa tttttcagct tgtaaagcaa tatcttgttt atctttttct
      901 gttaaatcat aataatggcg tttatcagta cgtcctaaaa gataatcagt tgatacatta
      961 aagtaatctg caactttttg tattttatct ccagatggag tacttttatt ccatctagac
     1021 atgcttccat ttccgaaatt taactttctt tcaagttctg ccaacgtcat tgaacgttct
     1081 tttaataaga tttttatacg gtctaatagc ataggatatg cattcctttc aaaataccga
     1141 caaaaaaagt atttttctgc aaattatcta tttacaaata gagaatttgc agttatacta
     1201 gttttgtaag caagatattt acggcataca aagactaaaa gcaataaact aatagtctcc
     1261 ccagacatca gtaagctcgc tttttgtgcg ctgtattttg tatgcttaca taatagcata
     1321 tttgcagttt ttgtcaaact tattagacaa aaatattaga aaatattcga taagggggta
     1381 tgaagcatga ctttaaaaca gcaagtatta attgctttag taaagaaagg ttggagtcaa
     1441 agagagttag ctcgacgaat gggtatatca attacttatt tacgagatat cttcatcgaa
     1501 aaaagaaaac ctaaggaacg tttaaaacaa atcgaagaac ttttggatat caagttagaa
     1561 gtagattcta atgatcagcc atcagaacag gaggcgtaaa aatggtacaa cgtttgagca
     1621 aagcggagtt taaagagaaa tataagtatt ctgaaagtac ttatcaaaga agaatgaagg
     1681 aatttaaaaa atcacgtttt agtgaggggt ataacgcagt tacttccaaa gaaataacaa
     1741 ttgatgtcga tttatatgaa cagtttagaa catggcgttc taaaaatcga tttagagtta
     1801 gagctgtttg ttaatagcta aaaaatctaa attcaccact tctgaaaaga ggagttgctg
     1861 ctctcctttc aagtttgcca aagtccacgt tttttcattt tggcaactcc tcttcttgga
     1921 ggtggtgacg gcgagagacg aaggagggat gacatgaagg aagaatcaaa aatagggatt
     1981 ttgaatgaga acaattacac aagattaatt attgaaacag aagaagggaa aaaagtagca
     2041 gaagttacat tgaccgatgc aactcctgct gacggttatc gtattcgatt aactcctaaa
     2101 tatgattaac cttttggtgg gtgtggatcg tttccgtgac tatcacggct ttgaattttt
     2161 ccatctctgc catgaataaa tagctcagat ccttgatttt tagaaatctc tctagcgatt
     2221 tttactgctt ctgattttgt attagtatgt gcagtcgctt tagaatttcc agcacctttg
     2281 acattccaac caccattgga attaggtaca acatgttgat tttttcccat agttttcacc
     2341 tccatttcta tagaaatatt ttatcaaata gaaatggaga aattcaataa acgaaaggag
     2401 aagtgatatg gcatatacct tgcaacaaga acatcaaatt ctcggtttga ttaaacaacg
     2461 caggaaacaa ttacaagatg accgtgcagc gcttagaaaa gccgatgagc tatcagatag
     2521 gcaagctgaa ctaattgctt ctgaacttga ggatttgaga atgctagaaa taaaaaatag
     2581 ggagattaga ttatgaagaa gacagacaca ctttttatag gattcatttt ggggttatta
     2641 gtgattgtag cgcaccaaag tattatcggg ggaagcttgt tcgcagcatt gatggtttta
     2701 atcaatctgc ttgattcaaa agaaaggagc aactatggca cgagaagaag cgctaaaaat
     2761 cggtaaagtg attgctgata atcggtgggg aaatagccgc cctattattt taagcaagca
     2821 acatatcgaa aagcaaaaag catggcaaca aataaaaaag tgactctgcc agccagcaaa
     2881 gagtcacata caaatttata aaactaggag aattatatca catgaataag aaaattgaaa
     2941 atcttatcga agaattgaaa cgtgaatgcc aaaagcaagg tgtttctatt atttgtacag
     3001 cccaaaaaga aggggaactt aaaagtctcg tttatggtga gacaacagaa attttacttt
     3061 gtcttgctat gcaagaagaa catttagatg aaaatctccc attgtcagca catattatgc
     3121 gtagaattgc tgtggatgct tacgaacaag cgaaaaatga agaagaaaat caaccttcaa
     3181 atcatacttt tgttattaat aacaaggaag atttagctga tgtgatgact cgtattctca
     3241 agggggaatt tcaatgatga atgaaaaaac aattgatcta ctcattattt tatttttcac
     3301 gttagtgatt gtagcgaaaa gttatgtaat ctttggcttg ttattcgcaa ttaccatgct
     3361 tgtgctttgc gtgttagcaa gcagaaaaga ggttaaacat gaacgagtta gcaaatcttg
     3421 acaactattt aactgatcct gattatgcta aaccaccata tgaagcacca attgatgagg
     3481 aggatgaaga tgagtaaatc taccttagaa atgagccatc aagaatggct tgaagaccgt
     3541 aagaaaggca tcggaggttc ggatgttgga acaattttag gattgaataa atggaaatct
     3601 ccttatcaac tatggctaga aaaaacagga caagtagtac ttgaagaatc aggaagtgag
     3661 cccgcttatt ggggtaatgt tttagaagaa gtggttgcta aagagtttca ggaacgtaca
     3721 ggcaaaaaag tacgcagaag aaaccaagta tttgaacatc cattacatcc atttttaaga
     3781 gcaaatattg atcgggaagt agtgggagaa aatgccattc tggaatgcaa aacagccaat
     3841 caatttctcg gtaaagaatg ggaaggcgaa gaggtaccgc ttagctatct ctgtcaagtt
     3901 caacattaca tgaacgttct aaacaaagac tattgttaca tcgctgtctt aatcggtggt
     3961 caaaaattta tctggaagcg gattgaacga gatcaagagc tgatcgatac aatcactgaa
     4021 caattagtag agttttggga aacaaacgtt cttggaggta tcgagccaat tattgatggt
     4081 agtcaagcga cagctgattt cttgaaagag aagtatgcag atgttgaaga cgttcaaacg
     4141 acgttaccaa ttcatttcga tgaactagtt gaacagaaaa acgaactcaa acggactaag
     4201 aaagaaattg aatcagctat ccgtcaagta gacaacgaga ttatcagcga gctaggtaaa
     4261 cgtgaagcca gtatcggtat cactcaaaaa aacatcatca gttggaaatt tgtccgtacg
     4321 agacgtatga actcgaagaa actagcagag aaatatccag atgtcgcaaa tgatgaagag
     4381 atttataacg ttactgaatc aagaaggcta accgaaaggg agatcaaata atatggcaac
     4441 aaatgaatcg ttaaaaaatc aattggcagc aaagccacag aaacaagttg caccaggtca
     4501 attaggactt aaagctttga tgaatacacc aacaatgaga aagaagttcg aagaggtgct
     4561 tcatgacaat gctaatgctt ttatgtcgaa tgttatgact cttgtatcta atgacagtta
     4621 tcttgcagat agtgaaccga tgtctatcat gagtggtgcg ttaactgctg caacattaaa
     4681 tcttgggcta gataagaatt taggttatgc atatttagtt ccattcaata gtaaaaacaa
     4741 gcaaacagga aaatgggaaa agaaagctca attcatgctt ggctataaag gatatatcca
     4801 attagcccaa cgatcaggta aatacaaagc attaaatgtg atcgaagttt acgaaggaga
     4861 actaaaaagc tggaaccgac tgacagaaga gtttgagttt gatccaaatg gtagaacgtc
     4921 tgatgaagtc attggatatg ttggctattt cgagttactg aatggattca aaaaaactgt
     4981 ctattggacc aaacaagaaa tcgaagctca tcggattgct aacaataaag atcgcgataa
     5041 gacaaagtta agtggtgtgt gggcatctga ttacaatgca atggcacgaa aaactgtttt
     5101 gagaaatctt ctttctaaat ggggaatctt gtccatcgaa atgcaagaag ccaccacatc
     5161 ggatgagaga gtccaaaggg ttcaagaaga cggcagcatt attgctgaaa cagaagttga
     5221 agaagatatt cctgaaagaa aagaagcaga ggttatatct gaagaaaatg aagatgtaca
     5281 aactggatta tttgatgcat ctaatccgcc gttaaacaaa taatgaggga gctttctccc
     5341 ttgcttttct agaggagaaa tacgaatgaa cacaggatat gtcaaattac atcgaaaagt
     5401 gatgaattca ttcgtttgga ccaatcctta tatgtataaa ttatggaact tgtgtttgat
     5461 gaaagcgagt catgagaatc gcaagatact ttttaatgga aaagagatac aagtgaacag
     5521 cggagaattc gtcacagggc gcgatgctat cacatctgag atgaacgaag gcgtgaaacg
     5581 tgaacatcaa gtgaacagcg gttctgtatg gagatggcta aaacaatttg aaaaaaacgg
     5641 aatgttgaac atcaaatcaa ctacgaaata cagcgttata tcaataaaaa actggtcttt
     5701 gtaccaaagc agtgaacaac aaatgaacat caaacgcaca acaagtgaac aacaagtgca
     5761 cacaaacaag aatgaaaaga attataagaa tgaaaagaat aataaaacat catcgtcgca
     5821 accacgcaaa aagcgtgttt acgacaccga ctcagtttac tacattctcg cggaggagtt
     5881 attcaaacag atttgtcaga atcaggaaat caaaaagccg aatctgcaaa gttgggcaga
     5941 tgacattcgg aaaatgatcg agatcgacaa gcggaccgaa aaccaggtac ggggaatgat
     6001 tgaatggagt cagcacaatg tgttttgggc atcgaatatt ttatctgcta aaaaattgcg
     6061 agaaaaatac gacacaatgg cagcgcaagc aaatcgtgat tataaaacaa aacaaactaa
     6121 aacgcttgaa tacgagaaat ttagcacaga tgagttgcct atttgagagg aggcgtaggc
     6181 atggaaactg ttggggaaat catggaaaag ctgatacaga aagtgcttgt ccaacgtggc
     6241 gaatgtcctg aatgcggaca gcctttgtat ggatggcgta cgaagaaccc tgatggctca
     6301 gaacgttgta agccaacctg tatgcaatgt gggtataaag cattgcgagt tcaggaagac
     6361 ttacaaacag aacgaattta caacgagagc ctgaaagcaa gagcaatcaa ttttttcaaa
     6421 ggtggttctg ttgtgcctaa tcaagcgttg tttgattgca cattgcagaa ttatcaaatt
     6481 gtcgatcaag aaacaagaca agcggttgaa gtaaccaaac gctttgttaa ttcagtcttg
     6541 ttaggaaatc caagtcacct tgttttaact ggaaaacaag gaacaggtaa aagtcaccta
     6601 gcgatggcag cggcttggga agtcttgaag cgatcaaact acgataaaaa aatcttattt
     6661 atcgggttac aggaaatgct ggatcaaatc aaattttcgt acaacaatcc tgaactcaga
     6721 aaaacgattg agggatcgtt gattgcagat atcaaaacag cggatttagt catcattgat
     6781 gatatcggtt cagaactagg aaaagatgca tcagatagtc gagcgtttgg cataaacacg
     6841 ctaaattcgt tcttggatgc acgacagaac ctagcaacga ttatcacaac gaacttgctt
     6901 ggtgaagaac tgaaaaaagc ttacggtacg agaacaatat caagaatgtt tgtcaactct
     6961 gatggattta cgatggtatt ttctcaaaca gcagacaagc gcataaaacc agtcaaagga
     7021 gcgatcgcat gaatttgact aaacaagaaa aaagaatctg tcgtcagata atgttgcaaa
     7081 ttataggtga tcgtgacatg gacgaattat ctcaacaaga gagtcgaatg gttcggaaga
     7141 taagtaagct aattatttat cgggatacag aaagatttct cactgtttta ggtaacgtct
     7201 acgagaatcc agagttattg gaggtagagt aattgccaaa aatattagat gcatgttgcg
     7261 gtagtagatt gttctggttt gataagaaca attctgacgt aacatttatg gattgtcgtc
     7321 agcagtatga agaattatca acaggtcatg tgatcaatgt tgatccagat gtggtcgcag
     7381 actttcgcga tatgcctttt gaagatgatg tgttcgatat ggttgtattt gacccgccac
     7441 acttaagaca tgtcggcgaa aactcatggc ttgccaaaaa atatggaaaa ttagacgaac
     7501 tttggccaga agacatcagg caaggttttg cagagtgtat gcgagtttta cgaccttcag
     7561 gaacattaat ttttaagtgg aacgaggaac agataccact ttctgatgtg ttagaggcta
     7621 ttggagaaca gccattgttt ggtaataaac gaagcaaaac acattggctt gtgtttatga
     7681 aaaaattcag ctaacggagg aaacaaagga ggaaaagtaa tgaagattgt attaaataaa
     7741 tgttttggtg gctttgggtt gagccatgaa gcaaaaatgg aaatttttaa aagaaaaaat
     7801 attgaagtat tcccatacat caataacttt agctatgact cagatgatga atacacgaga
     7861 tacacaggcc aaaagctagg ttcgatggat tttatctact atttcaaaaa agacccgaaa
     7921 atcgacaaag ttacaggaat ttatagcgaa attgaccgac tgtacggaat tgctgatgat
     7981 tccagttttt caagtgattc aaacagaggt gataaggacc tcgttgctgt tgtcgaaaaa
     8041 ctaggaagtg aggccagtgg accatatgca agtcttaaag tcgttgatat acccgatggg
     8101 gctgagtggg aaataagtga ttatgacggc gttgaaactg cacattatgg ttttcaaaca
     8161 ggtagtattt agtccactaa tgacaactaa agaggaggca gaaggatgag tgaagatgaa
     8221 tactttgaac agtagtctat taacgacaga gatttttaac tggaaagggg tgtagtcatg
     8281 gacgcatcgg aagtagtaaa taaaattaat agtctcaaag aggttttcgg agacgtcgaa
     8341 gtagtgataa acgcacaagg ctatgacaac gaatttttta agcagattac cgacattagc
     8401 attcagcaag gaatggaaga cgaagacgga aactttatcg atgaagtagc cattttagtg
     8461 acttgcgagt aagtaattga cagctaaaga ggaggaagcg gaatgaaact aaaagacgga
     8521 ttttactcca gcggccacgg catcggcggt ttaatgctag atatgccgac aaagaatcct
     8581 aaaacacgta agaaaccaaa attcaaagtc ggtgacatgg ttcgctgcga agcagaagga
     8641 ttcatctatc catttcgtgg atatgtagaa cacgtctata atcactcagc aatcattcgc
     8701 attgaaaaca cgatggaatg tgataagtgg ttagcgaaaa gcaaagagaa tttagcagtg
     8761 gtgagattgg tggacatgga agttataaaa tcttaatttt gcttttttac aactatccta
     8821 caacaatgag cgttattcca caactggaag gagaagaggc aattgtgaac attttagaga
     8881 atatagatat taagcaaaca aggaaaaatg ctagacgatt actaaaaaga tacagaaatt
     8941 tagaacgttt ggtcggacca gtgaaaatag acttttccgt gatgactgtt accaaaaatt
     9001 tgaaattcac aattgacagt caaaacgaag aaatcattga agcgataagt actcgagatt
     9061 cggttatcga agcattaaca cggctaagta gaatccattt ccaggtactt tattatagtt
     9121 attgttttcc taataagatg tcaatgtatc aaataggaga aaaattagga tattccgata
     9181 gaactatcga gagaatgaag gcagtagctt tggttgaatt tgctgaggcg tataaatcag
     9241 gagaactcat ttcacgtaca aaataaaaaa agtcggattc ctccgactat gaataatatt
     9301 tccgacataa gtattatatc ataattgggg gaatcagagg atggtacttt tcgatgtaaa
     9361 gaaatatgag acaccaagcg cgaaagatgt tgatatggaa cgcacaaaac ataatgtcgc
     9421 tgtgtttctt tcagcatatc tatcagctag atgtagagta gggcaacatc gagaaccaaa
     9481 agtgacagca tcttattcct tggttccacc ttctacagct gatcatatat ttgaagcaga
     9541 aagaatgctg atcgataaag aagaagcaca ggaagaattt gagtatctgc ataaattgtt
     9601 tgttcgaggc tattcagcga tacagcatcc gcataagcct gatgtgactg aaaggcgcaa
     9661 gaagatattc tatgatcgtt atatcaatgg tctgcccatt tatgtaactg ctcaaaggaa
     9721 taatactagc gaagaatcgg ttaaagtaga atcaaacaga attatcatcc aatttgcttc
     9781 atcgttagaa ctggttgctt tcaagtagcc agtttttaca ctttttatac ccttttatta
     9841 ccaatttggt ttccatttta tacctttttt ataccaatca cttacctatt caacgttgta
     9901 ttatgatagt gtcgaaaaat taggaaacag gatcgacaaa ataaaatgta agggaggaaa
     9961 tctccctcat cgtttaatta agcttcgata ggcagcaacg gaaatattaa gaataaggat
    10021 gtgaatttca actccttcta aattgttctt attatctatc atccgttgct gtctattgtt
    10081 tttttaatta ttcacacgat aactaaaggt aagtgaagag aaatgattcc attaataatt
    10141 tcaatttttg cgctttgtct taatgtatat atgattggat ttaaaaatgg gcaaaataaa
    10201 aaatagtagc agccaagaat aattttatag tgtcactgtg gcggaaaggg tagacgctaa
    10261 gcatgtgtgc taggtcaatg cttcggcaac catgcaaggt tcgattcctt gccagtgact
    10321 ttggcaaccg aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg tacgcgtttt
    10381 gtgcatcgtg taggttgcta ttacataact ggttaggtta gattgagatt tgggattcgg
    10441 tacaaatgaa tcgtcaaatg tctcaagcac aggatcggaa acgtccctgc ctgtgcatta
    10501 catattagat cactctttga gtggtctttt tctttacata aaggaggcta cataatgaga
    10561 aactactggt atgtatcgct aactaatgaa tatcctcgaa ccattgatga ttgttcagtg
    10621 cgtgttgtgc gttctgtaca aatcaaaggg aagtactcta ttgtcgaaat ggggagagaa
    10681 gcagaaccat gtgagattga tgcgtgcaag ctcgtttatt gcggtcatgg tttctatgat
    10741 gaaccaaaca ttcaaaataa tattaacaag aatttgaggg attagaatgc aagaaatggc
    10801 ttaaaaatct ccaatcagga caaatcaagt tagacaaagt ttccgattta aagatattga
    10861 ttgaagctga tttaatgcta aaagatattg aaaattagaa aacaaaactc aacctaaaca
    10921 gaatgtgagg tggtgtgtat tgaatggcaa gacaacgtga tccaagacgt gatgaagcca
    10981 aaagaatttg gttagaatcc aacggagaaa agcagttaaa ggaaattgca tctgaattaa
    11041 atgtttcaga ttctcaggtt agaaaatgga aatcgcaaga caaatggagc gctgaattga
    11101 ttagtaacgt taccaatggc aaaagtaacg ttactaatca aggtggcgct cctattggta
    11161 atcaaaatgc taaaggtaac aaaggaaata gccgagcttc tccgccagtg ggtaataaaa
    11221 acgctttgaa aacaggtgag tatgaaacaa tattttttga cacgttaacc gataatgaga
    11281 aggacatcta ttctagtttg aatgatgatc cttcttttgc tttgtccgaa gaaatacgac
    11341 ttcttaaaat aagacaactt cgaatgatga aaagaatcca acaagctgaa gctggactaa
    11401 atgatgaaga agtcgaacga ttgcagcagc taagaaagat taaaactccg attgaaaaaa
    11461 atggtaaaaa gctagaaatc aagcgtgagg ttatgcaaga tgtgcagatt agcagaaaaa
    11521 aacatcgcaa aattgatgat attctttcaa ttgaagattc attgactcgg attagcaacc
    11581 agttagctaa agccatcaag caaatgaatg aactttatat gaatgaatac agaactgatt
    11641 taattaaagc tcagactgat aagatccaag ctgagacaaa tgaaattggc ggaaataatt
    11701 caggtgaaga aatagaagaa tggaaacagg cagttttaaa tgccgcaaac aaacgggcgg
    11761 tgaaagaaaa tgaataatga atttattcct tttgctgata ttggttctgc cattgattat
    11821 tactatgata aaccagtagc tttttgccaa gatattttgc atttgaatcc tgatgaatgg
    11881 caagaaaatg ttttaaatga tttagctgaa ttttcaaagg tttctgttcg ttctggtcaa
    11941 ggagttggaa aaacagcatt agaagcagga gcaatacttt ggttcttaac gtgtcgaccc
    12001 tacgctaaag taatagcaac agctccgaca atgaagcaac tttacgatgt actttgggca
    12061 gaggtagcta aatggttaaa tgatagcttg atcaaaaact tactgaagtg gacaaagacc
    12121 aaaatttata tggttggtga ttcagagcgt tggtttgcta cggctagaac agcgactaaa
    12181 ccagaaagta tgcaaggttt tcacgaggac catatgttga ttgtggtaga tgaagcttct
    12241 ggtgtgtctg atccaattat ggaagctatt cttggtacgc tatcaggttt tgataataag
    12301 ctgttgatgt gtggaaaccc caataatatt gaaggtgtct tttatgattc ccataattca
    12361 gaccgtgata aatacagagt tcataaagta tcaagctatg atagtaaacg tacaaacaaa
    12421 gacaatatag aaatgattct taaaaaatat ggaaaagaaa gtgatgttgc tcgtgtccgt
    12481 atttttggag aatttccgaa aggtgcgttg gattcattta tcagtcttga aacagttgaa
    12541 ttggctacag aaaaacaaat tagtgattct ttagtcaata aaacaaaggt tgctcatatt
    12601 ggtgtcgacg tagctcgata tggtgatgat tctacgattc tctttcctag aattgctacc
    12661 agggcattgg agtatgagaa gtattcaaaa cgtagcacca tggaaacaac aggatatgtc
    12721 atcaacatgg ccaagaatct aatgagtcaa tatccgagta ttgataaagt gatgattaaa
    12781 gtcgatgaca ctggtgtcgg aggtggtgta accgaccgcc tagaagaact tatagaagac
    12841 aaacattatc cttttgaggt gtttggagtg aataacggtt caacatcaga agacgatttt
    12901 tacgataatt taggtactca actatgggga aacatcaagg aaatgttaga agaaaatatg
    12961 acagcaaatc ttaacggaga acagcctgtt attgaattgc cttctgatag ttcgttaatc
    13021 aaagaattaa gtactcgcaa attcaaaatg acaagtagaa gtcgtatacg tttagaaagt
    13081 aaagatgata tgaaaaagcg aaatattggt agtcccgata ttgctgacgc actggcttta
    13141 gcgttttatg agccaccaag tcactatcaa tttattcaat tttaggaggt gagcttttga
    13201 ttagtacagt attatcttta gagagatata agaagctacg tgttaaatat gcaacacaaa
    13261 ttgaagatgg aatgtttgat ccgaatggtt ttatagaaga tatgaaacca ttttttgctg
    13321 atcgcgaaag aaaatatcta gcttatacta gcgaaaagaa tgaaatagat aatagaccaa
    13381 agcctaacac tgatattgta aaagttaata ataaacttca tgctggtatg tattcaattg
    13441 tcgtagacca agcagtcaat cattttactg gtatacctat caaatgggat tacgatgtat
    13501 cggaacaaaa aagaacactc attcaaagat taaaagataa attcttaaaa aatgatatag
    13561 aacttccgac agttccagaa gcttttaaca aattaacaag caatcttgat tccatgagat
    13621 tcgcaatgct tgattctgaa actgcaactt ttcaaggagc ttgtggagta gctttccgat
    13681 tgctagagcc tgtggaagaa gatgatggtt ggaagttaag agcaagtaat attgaacctt
    13741 ggagagcaga aagatatgga aatgccggaa tctatatcaa agaaaagtat gactcttacc
    13801 agaaaaaatt ttttcaagaa atgaaagtta tgacaagaaa taaaattctg acatatgctt
    13861 gctatggtga tttgaatttt gttacaagcg gaacgttcaa aaaaattgat gagaccgata
    13921 accctttggg gacgattacc ttggcagaat ttaaaaataa tacgaatcgt tattgtgatt
    13981 ttgaagtagc tgaggaaatt ggagatgcaa ttgatcgtgc tttgtctgat caacaaaacg
    14041 aaatcgaaca atttaagctt gcgtacatgc ttgttactgg cacaacaatg agcaaaggca
    14101 ctgctaaaga aatgatgaat cagctaggga ttatcaactt aaaagcccct actgcaaaag
    14161 ctgaatatgt cacaaaaaat ttagcaaaag atttcaatga atatcatatg gatctattga
    14221 aaaaacagtt ttacaccatt tgtaaagcaa tcgattttaa cgatgaggtg tttaaatcaa
    14281 acagttctgg agaagctcgc aagtggcaaa tcattagcct agaagcgaaa acaaatacta
    14341 aagagcaata ttttagagaa ggattgaaag aatgcgcaga aacaattgct gcttttctta
    14401 agtttcatga caaagtagaa attgagcctg aaaaaattat ttttactttc tctagatctt
    14461 tgccaactga tttaagttat ttagctgaag ctttacctaa acttgcacca tacgtatcca
    14521 aacgaactat tcagagccaa attccatttg taacagatgt tgattatgaa aatgaaatga
    14581 tggaattgga aagtggaagt gcttatccag atagtgaata caattttggc ggaggtggca
    14641 atagtgacga tagaaacgaa gtattggacc aaacgtcgag aactggagga tcaagcaagg
    14701 ctcaaacaag aaaatcagac acttaaaaaa ttaactagtg tatttcctga agcactgaaa
    14761 gagatacaag caaaactatt atcacaagct gacttacaca atatcactta tccagaaatg
    14821 atggagtttt acagtacaag taatcagaaa aaatatcgtg aatatgtgga aaaaaattat
    14881 aagtcattaa aaatgtatga tgcaaaatac aaagagttta tcgatgaatt ttttccacca
    14941 tttgactatg caaaagtcaa tcgcttatta caaatacgat cagatgtatt taaaattctt
    15001 gcagaatatg cgatggatgc agatgtgaat caatattttt ctgatcgctt agaggaaatt
    15061 cttcaaagaa catattcttc taatgctaat gtttttgttc aacttttaaa cgttgatata
    15121 ccaaattatt taccagaaaa tgaaattaaa cattatttga actatccatg gtcaggaaag
    15181 acattttcaa gaagactttg gggaaatatt tcatcgcttg aacagaaact ctctaatgct
    15241 attgtaaaaa gtgttgctag tggagaaggc gttatacacg cattaaacac catgagatta
    15301 gactcagaaa tttgtgacat gtttaagtta gaagaatcaa agtataacaa agcgatagaa
    15361 aatctcgttc gaacggagta tgcaaaattt gcacaagatg gtattgaaaa atcatattta
    15421 gaaacaggta ttgaggaata caacgtattg actgcaaaag atgagagagt ttgccggatt
    15481 tgtggaggaa aggcaagtaa gaatccctat aaactgaaag atgctgtcat aggtgaaaat
    15541 cgagcacctt tccatagtcg ttgtagatgt acggatgttc ctaatttacc aaaattagga
    15601 aaggatattg atgaagaata tgaccgttta tttggcgatt tattagatga gtttgcacat
    15661 gattcttttg gaattaattt gaaacggagg aagtagaatg aaagattttt ttgaagcagt
    15721 actaacaatt aatgtaaatg ctgatattgc agaagcctac aaaacagcta ttgagtctga
    15781 gaaccatcct aatggcttga gagaccattg gaatggcaat tatgcctacg tggttattgg
    15841 cgatcaaact gttaattatc aagataatac tccagttgat aagaataccg ttaatttaac
    15901 gattcaatta ttatctcatt cattaccaaa tttaaaagaa acagttgatt ggtatgaaaa
    15961 gatgggatgt attgttgtta gaactgacta caaagaagga aagtctagta attaggcttt
    16021 tttattttgt ccgaaatgac gttaaactag cgcaatactg ggcttggttg aatggtgggg
    16081 cgcaactatt aaaactcaaa gcaatgcggg gcgtgaaaac gaatcgtggg gcgaaaggag
    16141 aatgagcatg aaacacaaat cattaatgcc aatgaatttg caatactttg ccgaaggtga
    16201 cgatcaaaag ttttcttttg atgacttcaa atcttttgtg gaatccaatg aagaagctca
    16261 aaaatttatt cagtcacaat ctcaaagtgt tgccgacaaa caattggaag cttggaaaca
    16321 aaataactta gataaaatta aacaggatac catcaaggaa tatgaggaat ctaagaaaaa
    16381 caagtcacct gagcaaattc aactggaaaa actacaagct gaatttgaag cagaaaaagc
    16441 gttgcgtgtg acaagtgata ataaagcatt tgttgcagaa caaattgctg gattagaact
    16501 agatggagag ttaaaagagt ctatttctca atttatgcta aataatcttg ttagttcgga
    16561 tacagatttc actaagaatg ctgttgaagg ttttacaagt gttttgaatg caattaatga
    16621 gaaacatgca gatgcattaa aagaactaca aatgaagtct gcatttggtg gaactcaaca
    16681 atcgaataac caggttcagc agaacaatga aacattaaca aatccagaag aacaattagg
    16741 acaaattctt caacaattta actaggagag tgaaaaatta tgaaaaaaac atctttaaat
    16801 aatttagagt atttggatat ttcaccagcg attaatgcta tgcaagtacc aaatacacct
    16861 tttttaagct atttacttgg tgctggaaaa acagagccag caaactcgac agaaattaaa
    16921 tggcgtgaat atgatatcaa caacgatgat tcttctgaaa aacttgaggg cggagaatat
    16981 ccagatgctg aatcaggtcg aacttggttt aacaactata ctgaaatttt tagaaaatca
    17041 acctctgtat ctggtacatt agatgctatt aatgtgaatg gagtcggaaa tgaattaact
    17101 aatcaagtag cactacgtgg tatggaaatg aaaattgatt tgaaccgaaa attgattact
    17161 ggtgtaaaag ctgatgaaaa tggttctaaa ggtcgtcgaa tgaatgggat tttgaacttg
    17221 atcaattcag caaataaggc agaaacagcc actgcgggtg cagtaacaag aaaagatatc
    17281 gatactttat ttaaattgat gtatgaaaaa ggttatatgg gagaaaaact atgcttgatt
    17341 tctccagata tgcaggagtt aatgactgat gagttagatg gaaaatcaac aaaaattgtt
    17401 cagttcgggg aaagagtaac ttttggattg caaattggaa atatcgtgtc taattacggt
    17461 acaggtattg ctctactaga accatcattg ccaaaaggaa caattgccgc aatcgatact
    17521 aattatgtga aactacgtcc attacgtgaa tggagagcag aagaacttgc aaaaacaacg
    17581 gattctagac gtatcggtct tgtaggtgaa tactctcttg aatacaatgc ttcaaactct
    17641 ggggcaattt taaatttaaa gtctgaataa aagggagtta gtactccctt ttttgatagg
    17701 aggaattaca gtggtaaaaa aagatgaaac taaaaaagat gaagtcgtga aatatagagt
    17761 aggtaaaact aaaaattttg ttgggtttgt tcatcctaaa actcgtagat ttatcacagc
    17821 agattcaaat aacgaattta tcatttctat agatgataaa gaagcaattg caattttgga
    17881 agatgcaatt gatgttaatg aaatttagga agtgatctga tggatgaatc gctaaaaacg
    17941 gaaatcattg agtctacaaa agaagatttt ccagatttga gtgaagaacg cataactaat
    18001 ttattagaaa taattttgct agaaattgaa tcatacaaca cttgtaaaaa tgatatttca
    18061 tgggaaaagt taaaaagcgt gattagtgaa gtgttgtata aaataataaa aaatgaatca
    18121 gaaaaaacag tatcttcagt tagacgtggc gatacaacga ttagttatgc ttcaacaaca
    18181 aatgatgtta gtgagttact tctaggctat ggtgatttga tacgaagagt tattggttgt
    18241 ggaggattgg agttttttta atgaatgaag cagatatttt agaaactact tatgaagata
    18301 gttgtattat tgaaagactg acggacattg aagattctaa tacaaatatt actattcaag
    18361 attacaaaaa agtatatgat aatcctattt cttgcgctct ttcacaaggt caaatcgatg
    18421 gactagcagt catagaagat ggagagatgg taaatgtttc aactgacaca tataaattat
    18481 ttgttcatcc taagattaaa ctaaaaaaag gagatcgaat aacaataact caaaaggcca
    18541 gtggcttaat tttttctcta tttgctacta agccttttta ctatcctagt cattgtgaag
    18601 tgaatttgat aggaagtgag aaaaatgggt aatctcaaat atgaatcaaa tgcagaaaag
    18661 atcattgaga attttaaaaa tatgactgta attgctcaaa aagaaggaat atccttcgtt
    18721 aacgattcaa tgaataaagt cgttagttta gctaaaccat taactcctgt aaaatcaggt
    18781 aatttaaggc gaggatatcg tgtagtaaaa gctagaaaac tatcaagcgg tcgaattgtt
    18841 ggagaagtta taaacaatga acattatttt aaatatgtag aagaaggtca taggactaaa
    18901 aatggtggct ttgtcaaagg gaaattcatg ttaactcgtg caacgaatct tgcaaatatg
    18961 tcttatattc ctcgaagatt taaacaaatg gcaataaaaa tcgttaagaa aggaaagtaa
    19021 catgaaagat gaaatcattg ctgcaatcag tagcaaatta aaagaaatct atcctgatgg
    19081 gacaatatat cttgattcag ttatgcagtc aactaaagat ttttatttcg ttttatcagt
    19141 aatggaatct ggaactgaaa atgtaggaat tgatattcaa aatgtttctt tcttaattga
    19201 tattgcattg attgataata accctaatag aaatttaatt aatgaattag tctcacgttg
    19261 tgggactttt tttaatacga ttacaatcga tgagcaaata ttatttccaa aagcctattt
    19321 acctgatgaa gcagatggtg ttcaacatat tcgttttaca ttagaatttc cacaatatat
    19381 tgaatggagt gaaagataaa tgggagaaaa aagaagtaaa accggaatta tttctgtcga
    19441 aaaacctact tggtttcctt tggaagatga gacagggaat tttccggtat atggtactgc
    19501 gttgccaatg ggaacagcag taagtatcaa accaacagct aattatgaaa caacacaaga
    19561 ttatggggat tcagtggttc aagatcaatt tacagctttt ggtggtgcag aagttgaact
    19621 ggaagcaaat ggatatactc ataaagtttt atctacaatt actggaggga aaattgttaa
    19681 aggtggtgcc ttgcgttctg gagaagatat tgcgcaagat ggtgcgtttg catatagacg
    19741 taaaaaatcg aatgggaaat accgttatac ggtcttttat aaaggacagt ttgctctaga
    19801 ttcagatgaa acatcaacga ttgaaggaag taaagtaagt tttactcatc caacttggaa
    19861 gggttcattt gtagatgtac caggacttgg atatatgtat tcagttgatg aagatgatga
    19921 aggcgtggat aaagcaatga tcggaaattg gtttacaaaa gtagcaattc caattgaaga
    19981 agcagaactt tcaggaggta cagaataatg tcaaaatatc aaacaacgat taaattaacg
    20041 aaaaaagacg aagaaggtaa atatgagcaa gtacaattta aatctgctga atttttacca
    20101 ggaacagttg tagaagatgc tgcaggagtt atggaagaaa tgcaaacagc gactgataag
    20161 caatcagtta aaaaagcttt aagtcgtgct tattcattta ttgcagatac tttatttgag
    20221 ggacaattta ctggtgaaga ttattgcaaa ggaattgatg ctcgtgagat tgcctcattg
    20281 acaggaaaat tattgaagtc tgttactgca ggttttgatg aaacttatac agagacgaaa
    20341 aaaaagtaag tgaggcgctc aagtcacctt catttaagta ttcgattact taccgagaat
    20401 tagatataaa gacgcaatta ctcgaagcag gttggacgtt accagaaatt gaacgtactg
    20461 acttggatga gttaatgcgt ctttttgctt ttagagatgc ggtaaaagag catgaagatg
    20521 ttgagtacta tgataatttc actcaatttt aggaggtgat actttgaata acgatgacct
    20581 aattctgaag atgatactag atgaatctgg ttttactgcc ggtatgaata atgctgtcaa
    20641 aaagttgaac tcttttgatg aaacaatcga aagaaaaagt agaaatagtg gcagttcatt
    20701 aggtagtatt tggaaaatat ttgctggaag ttttctagct agcggagtaa ctagaatcgt
    20761 aggagctggc tttgatctaa tcaagggttc cataagtgga gcggttgatc gagtagatac
    20821 gatgaataac gctctacgaa atttccaaaa tatgggattt agtaattcag aaattatgaa
    20881 gaatatcgga aagaatgggc ttttatctca aggtattcaa ggacttccta ctgctttgaa
    20941 tgatgcgata agtcatgttc aacttcttgc ttcttctaca ggtgatttaa ctcgttcaac
    21001 tcaaatattt aaagctttaa atgatggaat tcttggtttc ggtggttcaa ctgatcaagt
    21061 taatgaagct gttattcaat tatctcaaag tttctcaaac ggaaaagtag acgcacaaac
    21121 ttggaactca atgatcaacg ctcaacttgg acctactctt tctgctattg ctaaaaagat
    21181 gggaattaca atgggagatc tgaaagaagg tttatctcaa ggtaagattt ctgttgaaga
    21241 attccaaaat caattaatag aaatggatac caaaggtggc ggaggactta aatcattaag
    21301 tcaaatcgct aaagattcta ctaagggaat taaaacctct atacaaaatg caaaaacagc
    21361 tgttacacgt ggtgttggtg aggtaataga aggattaaat aaagcattag tagattcaga
    21421 tttaggtgga tttaagggaa ttattgataa agtgggcagt tcaatggaat cgttcttaaa
    21481 agtaattgct gcaaatattc ctacagcagt atcattttta agtaatttat ttaacgaaat
    21541 tcaaaaattt ggttctgctt tgaaattcat gatgcccttt cttgttcctg cagctaccgc
    21601 ttttggagcg ttcatgtttc aacttaaagg tataccagca ataataaaaa gcttcaataa
    21661 ttttaagaat gccataattg gcgttggaaa ttcattaaag attatggggg caatagctgc
    21721 tgctaatcca tttgttttaa ttattggtgc agtcgttggt gcaatagctg ttttcggtta
    21781 ctttatggca accaatgaag agcttagaaa caaggttata tctatttgga atgatgtgaa
    21841 agattccgta attggtgtat taaaaaatat aaaggactgg ggaattgata cttggaattc
    21901 tgctaaagaa atggcatcaa atgcagttga gggtgtcaaa gatgcttggt caggaacaaa
    21961 ggaatggttt tctaacacct ggaaaggtat taaggatggg gccacaggtt tatttgataa
    22021 aacagtagag acttcaagaa atgcagtcga tagtgtaaaa aatgcatggt ctaatatgaa
    22081 gaaatggttt tctgatactt ggcaaagtat aaaagattca gccagagaaa aatggaatgg
    22141 aatcaaaggt tctattatgt cagttgctgg tccattgatt acaggtataa agaatgcatt
    22201 tttacatgtt actttttatc tagagacctt atggaataat ttagttgaga ttggtaaaaa
    22261 cgtctttgag attttaaaaa atgttatact tgcgccggtt ttgtttatta cctccatgat
    22321 ttctggtgga tgggaagaaa caaaaaataa tatgattgct gtttggaata atattaaaga
    22381 aag
//
LOCUS       DAYYBG010000043        22159 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000043 DAYYBG010000000
VERSION     DAYYBG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.043"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            229..2064
                     /gene="tdc"
                     /locus_tag="ACHAUN_001559"
     CDS             229..2064
                     /gene="tdc"
                     /locus_tag="ACHAUN_001559"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8460263.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2369..3847
                     /locus_tag="ACHAUN_001560"
     CDS             2369..3847
                     /locus_tag="ACHAUN_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8460264.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3813..5144
                     /locus_tag="ACHAUN_001561"
     CDS             3813..5144
                     /locus_tag="ACHAUN_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8460265.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5258..7810
                     /locus_tag="ACHAUN_001562"
     CDS             5258..7810
                     /locus_tag="ACHAUN_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8460266.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7817..9256)
                     /locus_tag="ACHAUN_001563"
     CDS             complement(7817..9256)
                     /locus_tag="ACHAUN_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8460267.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9231..9413
                     /locus_tag="ACHAUN_001564"
     CDS             9231..9413
                     /locus_tag="ACHAUN_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460268.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9602..9964
                     /locus_tag="ACHAUN_001565"
     CDS             9602..9964
                     /locus_tag="ACHAUN_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8460269.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10151..11008)
                     /locus_tag="ACHAUN_001566"
     CDS             complement(10151..11008)
                     /locus_tag="ACHAUN_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8460270.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11128..12039
                     /locus_tag="ACHAUN_001567"
     CDS             11128..12039
                     /locus_tag="ACHAUN_001567"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8460271.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12046..13692
                     /gene="mdcA"
                     /locus_tag="ACHAUN_001568"
     CDS             12046..13692
                     /gene="mdcA"
                     /locus_tag="ACHAUN_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM8460272.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13794..14093
                     /locus_tag="ACHAUN_001569"
     CDS             13794..14093
                     /locus_tag="ACHAUN_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8460273.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14083..15759
                     /gene="mdcD"
                     /locus_tag="ACHAUN_001570"
     CDS             14083..15759
                     /gene="mdcD"
                     /locus_tag="ACHAUN_001570"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8460274.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15763..16356
                     /locus_tag="ACHAUN_001571"
     CDS             15763..16356
                     /locus_tag="ACHAUN_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8460275.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16380..17456
                     /locus_tag="ACHAUN_001572"
     CDS             16380..17456
                     /locus_tag="ACHAUN_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8460276.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17481..18350
                     /gene="mdcB"
                     /locus_tag="ACHAUN_001573"
     CDS             17481..18350
                     /gene="mdcB"
                     /locus_tag="ACHAUN_001573"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8460277.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18461..19225
                     /locus_tag="ACHAUN_001574"
     CDS             18461..19225
                     /locus_tag="ACHAUN_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8460278.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19714..21807
                     /locus_tag="ACHAUN_001575"
     CDS             19714..21807
                     /locus_tag="ACHAUN_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8460279.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21832..22134
                     /locus_tag="ACHAUN_001576"
     CDS             21832..22134
                     /locus_tag="ACHAUN_001576"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8460280.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3785 c         4721 g         6476 t
ORIGIN      
        1 tttttttagg ttatctattt tagaagactg atttattgaa aaattgttcg cttttttgcg
       61 ttgaattatc agaaaaatcc gactttattt atttttgttt gtaaaaatct acgtggattt
      121 gatgttataa aaaaaaaatt ctcatttttg gcgctatagt gaagaggtgt tacagataaa
      181 cacatgtatc tgcaagacat tgaccaacaa aatatggagg gaagaaaaat gaaagatatg
      241 gatatcaagg ccgtctttat aggcgacaaa gctgaaaatg gaccagttta taaaatgttg
      301 ttgaacaaga tggtagatga acatcttgga tggagagaaa attatattcc ttcagatatg
      361 cctgcaatta gcgaaggaga taagctcact ccagattatc tagccacccg cgaccatatg
      421 atagaggtct tggatgaagt cagtcagcga ttacgcgcag gatcgatccc atggcattca
      481 gcaggccgtt actggggaca aatgaatgct gaaacattga tgcctgcttt attagcttac
      541 aactatgcga tgctgtggaa cccaaataat gttgcattgg aatcttctat ggcaacttcg
      601 caaatggaag cagaagtagg acaggatttt gcttcattat tcaatatgac agacggatgg
      661 ggacacattg cagcagatgg ttctattgct aatcttgaag gattatggta cgcccgttgc
      721 atcaaatcga ttcctttagc tgtcaaggaa gtgctgcctg aaaaagtgaa aaaaatgtct
      781 gagtgggaac ttttgaattt atctgttgaa gaaattttgg aaatgaccga aagctttacg
      841 gacgaagaaa tggatgaggt caaagcagct tcttcccgta gcggaaaaaa tatccaaaga
      901 ttagggaaat ggctggtccc tcaaacaaaa cactattctt ggatgaaggc gttagatatt
      961 tgcggagtcg gcttagatca gatggttgct atcccagttc aagaggatta tcgaatggat
     1021 attaatgctt tggaaaaaac aattcgtgaa ttagctggac aaaaaatccc aattctaggt
     1081 gtggttgctg tcgtaggaac gacagaagaa ggtcaagtag acagtgtaga taaaatcgtc
     1141 caattaagag aaaggttaaa agatgaaggg atctatttct atttacatgt tgatgccgca
     1201 tatggcggct atgcgcgttc actgtttcta aacgaagcag gagaatttgt gccgtatgct
     1261 tccttagctg aattctttga agaacatcat gttttccacc actgcgtaac gattgacaaa
     1321 gaggtatatg aagggttcag agctatttca gaagcggatt ctgtcacgat agatcctcat
     1381 aagatgggct atgtccctta tgctgctgga ggaatcgtaa tcaaacataa aaatatgaga
     1441 aatatcattt cttatttcgc gccatatgtg tttgaaaaat cagtaaaggc tccagatatg
     1501 ttaggagctt atattttgga aggatcaaaa gctggagcaa ccgccgccgc agtctggaca
     1561 gcacatcgtg tattgccatt gaatgtgacc ggatatggtc aattgattgg ggcttcaatc
     1621 gaggcagctc aaagattcag agaatttctc gatcatctga cttttactgt taaaggaaaa
     1681 acaatcgaag tctatccatt gaatcatcct gattttaata tggtcaattg ggtattcaaa
     1741 gaacaaggct gtacagattt gaacgctatc aatgaattaa atgaaaaaat gttcgatcgg
     1801 tcttcttata tggatggcga tgtttatggt gaacggttta ttacttctca tactacattt
     1861 acacaagaag attacggtga ttctccgatt cgttttgttg aaagaatggg tctaacgaaa
     1921 gaagaatgga aaaaagaaca gaaaatcact ctgctacgtg cggctatcat gactccttat
     1981 ttgaatgatg atcgaatttt caatttttat acaaaaaaaa tcgccaaagc aatggaaaag
     2041 aaactaaacg aaatcatcca atagaactga tttttgtgtg aaagaataac aagagacata
     2101 tggagaacag acaattattt gcttcatcaa aacaattgta tttccccctg tttttcatat
     2161 attcctcgcc aagatattct tttttataaa acgttctata agttggatcg ttcgtttcat
     2221 gtatcaaacc cagttcacta agatcgttca atggatgttt cggtgatact gggttttgta
     2281 ctgaatcgat gaaaccttgt catttctgtt cttgaacaaa aataaaagaa aacaaaaaca
     2341 tatgtcgcta ttgaaagaag gaataaagat gaaagatact ggttcatcaa agcaaatcac
     2401 gaccatgacc tttatcggaa tgacctgcgc ccttgtagca agtgtacgaa atattccaga
     2461 tgtagcagca acgagctgga cgatgatttt ttacatgctg gttgcagtat tattgtacgc
     2521 ttttccaatc tcattgatct ctggtgaatt tgctggaatg tttccccaaa aaggtggtcc
     2581 agaactttgg gtatcgaatg ctctaggaaa aaaatggggg ttcgttgtct catggctgtt
     2641 atgggtccaa atgtttcctg ggatggtgat ggtcgcttca gcactcgctc cgttgtttgg
     2701 taatattatc gataatgtgc cgcttggttt gaacagcaaa tttacattag ttgtcatttt
     2761 agtcgtttat tggatcatca cttttttgaa tttgaaattt gatatggcaa aaatcggcgg
     2821 aaaagtcggt gtttggttag gattgtacgt gcctttgacg atgatgctct tattagggtt
     2881 tgctgcgtgg atcaaagttg ggatcgtacc aaccggaaca ttaggcagtt tttcatggga
     2941 caaattaata ccagatacta cgactgcatc ctcatttgtt tattttgcgc cgatcatgtt
     3001 tatttttact ggtatcgaaa tgtcctctgt gtatatcact cgattggaaa atccagttaa
     3061 aacgtatatt cgcggagtat ttgccgcgtt aatttttata tttttcgtca atattttgaa
     3121 tgctttagtt gtagcaaatg tcgtaccaaa aggacaaatg gaattgaata atatcgcgca
     3181 atctatttcg atttattgcc aaattcttgg tttgccacat cttattgtga atctgtttag
     3241 tttacttgtc ttcattggtg tagcagtcca attatctgct tgggcctcag gaccagcaaa
     3301 gacagtgaca gaaagtgcta gaaaaggtgc gtatccacca aaattcaatt tttggaagac
     3361 gaatcaattt gacgtatcca aatccgttat tttgactcag tctgtcatta tctcaatctt
     3421 tgcacttttt tatttgctga ttcctggcgt caatcaagca tttttgatgc tcgtaaattc
     3481 aactactgtt atctactgta tcgtctatgt catcatgggg atcgcagttt tgagattgcg
     3541 ttatacacat gcaaagttga atcgaccatt tagaattgga aaaaaagaga aaaagagcaa
     3601 tcttggagtt tggactgtcg ttattgtttt atttgcggca atcggatttt ctgtcggact
     3661 gacgatgaaa gctggaacat ggatcaatct aattgcagtc acagccatca gtgtcatttt
     3721 gtttgtagta ccactctgta ttgaaaaaat aaagaaacct tcttgggaac aggaagtcgc
     3781 catgctgctt gaaaaagaaa aaggtgaact tgatggaaaa accggcaatg aaacggactc
     3841 taactaatcg aaatatccag ttaatcgcat taggtggcgc aattggaaca ggattatttt
     3901 taggttcagc aaaggcaatt gcatcagcag ggccagctgt tattctcgtt tatttgatat
     3961 gcggtttggc tatttatggt ttgatgcggg ccatgggtga tttattttta gcaaatagta
     4021 catttaactc tatcagtgat ttcgttgact attatttagg caaaaagtgg gcattttttg
     4081 tcaattggac ttattggctt tgctggcttg gagctggact tgcggaatta acagcaatag
     4141 gattgtatat ccatttctgg tttgtacatg taccagcttt gataagcgga ggtagcgcac
     4201 taatgatctt aatggcagtc aaccttgttg cagtcaagtg gtttggtgaa ttggaatctt
     4261 ttttctcaat catcaagatc ttggggattt tcctttttat cttattgggt ataggattga
     4321 gtgtctcaga ttttcatacc cagtctatcg gaatcaaaga aaattttgga cagatcgatg
     4381 aattactacc gtttggcttt gctggaatgg tagtctcatt tcctatggtt ttattcagtt
     4441 ttgctggaat tgaaatgatc ggcttaaccg ttggcgagac aagtgatccg caaaagaatt
     4501 tgaaaaaagc aatcaactgt ctgccttggc gtattctctt tttctatata ggaacgattt
     4561 tgtcgatttt atttgttgtt ccatggcatt gtttagattt aaaagaaagt cctttagtga
     4621 ctatgctgca agtgatccat tgccaaggcg gagcagcaat tgtcaatcta atgattttgg
     4681 ttgcttcttt gtcctcagca aatagtgcag tctacagtac tagcaggatt ttatatcaaa
     4741 cagcaaaaga aaaaagtttg ccccattggt tttgtgcgct ttctaagaaa ggcgtacctt
     4801 tacacggtat tttaattaca ggaatgttat tttttgccgg tctttgtcta catttttttg
     4861 tagctgatag tcgattgttt tttcagcttt tggcaggcat gacaacgagt tgttttctat
     4921 ttgtgtggtc ttcgatcttg tgtgcacatg ttcaatttat taaagaaaag aaacggaaca
     4981 ctacacaaag atataaagat cttgctttgt tggtgttttt tacttcaatt gctttcagct
     5041 tgttattcga aagcacgaca cgctttattc cttttttatt ggttatctgg tttgtattct
     5101 taaattttgt atataaaaga ataagcaata gaaatatcag ttaggcccag atacattttg
     5161 caaaacgaat tatttcagac ccccctgaaa taatcgatac gcctgaaaaa acttccctgc
     5221 gtataataac agtatcaatt aaacaggagg ggataggatg aactggtaca gattgacggt
     5281 agaagatgta ttgaaaaact atcaaacatc aacagaaggc ttgtcgcaag aagaacgaca
     5341 gaaacgcctg actgatcaag gatataatga aataaaagca aggcagcaga caaaacgatg
     5401 gaaaaaaata gcaaagcatt ttactgatct gctgatgatc gtgttgatca ttgcctcttt
     5461 tttgaaattt gcttctagtg aatatattga aggaagtatt attttattcg tagtgatcgt
     5521 caatggactc gttagttact ggcaagaaag aaaagcggaa gaatcattga atggtttgaa
     5581 gcagctgatg ggacaagaag cgatagtact atcaaacggg atgcaagaaa aaatccctgc
     5641 gaaagaactg gtaaaaggag atgtcattgt cttacgtgcc ggagatgtcg ttccagcaga
     5701 tatccgatta atcgaagcac atgatttatt agtggaagaa tcgattttga cgggagaatc
     5761 agaagcatct gaaaaaagtc atgccgtttt gacagaagaa gaatcgatcg gcgaccagaa
     5821 aaatatgggt ttttcaggaa cgcttgtaca atctggttct gcagtcggtg tcgttgtaga
     5881 aacaggaaac acgacagaaa tcggtaaaat caatcaagca ttgcaatcgg tcgaacaaca
     5941 aacgacgcct ctgattaaaa aaatcaaccg attaaacaaa caaatttttc aaggcattct
     6001 ttgtttgagc ctattcttag ttatttttac gacatttcga tatggtatgg attggcatat
     6061 ccttctttcg gctacgattg ctttggtggt gtcaatggta cccgaaggat tgccagctgt
     6121 tttgaccatg attctatccg ttggagtaca tgaaatggca aaagaaaagg cgatcatcaa
     6181 aggacttcct tcggtagaaa cattaggttc tatgacagtg atctgttcag ataagacagg
     6241 aacattgaca aaaaatgaaa tgacagttgt ggatgtagct gcaaaagaag aagcctgcgt
     6301 gttgtccatt atgaaaaact gtcaggaatt aaaaacaaaa gaagaacaga aaacagaaaa
     6361 tttaagtggc aatccgacag aagttgcgct gattcgacac acagaaacag ccaatctacc
     6421 tttgaaaaaa atagaagcaa agattccgtt cagttctgaa tacaaatata tggcaacgat
     6481 gcatgcggag gaaaaaggag cggtgattta cgtcaaaggt gcaccagaag tattatttgc
     6541 taaatcaact ctttcttctg cagaacaaga agcatggagt cagacagctg cagaatttgc
     6601 gagaaaaggt cagcgtgttt taggatttgc ttataaaaaa gtagattcga aacaggaact
     6661 cacgcatgag actttaaccc aattaacttt tgcagggatc gccggattga tcgatccgcc
     6721 aaaagaaagt gcagtcaaag ctgtaaagga atgccaacaa gcagggatct cagtgaaaat
     6781 gattacaggg gatcacaaag acacagcaaa agcgattgcg gaacaaattg gcttaaaaca
     6841 tactgccaaa gttttagaag ggatcgattt ggatcttatg tctgatgaag agttgattca
     6901 gcaagtgcca atagtcgatg tctttgctcg aacgacacca gaacataaac tgcgaattgt
     6961 taaagctttg cagaaaaatg gagaaatcgt cggaatgacc ggagacggcg taaatgatgc
     7021 acctgctttg aaaagatccg atgtaggaat tgctatggga atcaaaggta gtgaagtaag
     7081 caaacaggca gctgatatgg ttttaggaga tgataatttc catacgattg caaaagccgt
     7141 aaaagagggc cggcggattt tggataattt gcaaaaaacc atcaatttct tcttgccaac
     7201 tgcattagct caaggattga tcttgatttg ggcacttatg ctgaatcgcc cgctaccttt
     7261 gtccccagta cagattttat gggtcaatat ggtaacgaca atcactttat cttatgcact
     7321 agggttcgag tcgccagatc cagaaatcat gaagcgccca ccaagagatc caaagcaagg
     7381 aatcctatct ggttatcatt tgtttcggat catctatgtc tccctactga tcatggttcc
     7441 tgcctattta ttggctatgc agtttgaagg gcaaagctta cagcaaacca ttcttctgca
     7501 aaatatcgtc ttagctcaag ctgtttacat gatcaattgc agagagttgt ccaagccgtc
     7561 attgaaccaa ggtcttttgc aaaacaaagc gctttttctt tcattaggta tccttgcttt
     7621 attgcaaggg atggttattt ttctaccagt cggccagcag ctgatcggta cagtaagttt
     7681 aacgatgaca caacagttat tgattggagc aaatgtgatt cttctattct tagttgtaga
     7741 aatagaaaaa gggatcatga acaaactgtt tcgcaaaaaa gaaaaaacag ctagagaata
     7801 cagtcgctag ccgttactat aaaaaagttt tgaaagtgag ttcagtcaaa cgattgagct
     7861 catttttcgt tggaatagaa gaaatgtata caatagggat atctagagaa tctagaggga
     7921 aattggaaat caagatatct gttttttcaa aagaaatagt ggaaagatcg gttagttcga
     7981 taggtaagaa gcagacacgt tttcctaagt aatcttttaa ttcctgctgc aagaacgcct
     8041 tccatgaagg ttctccttgg aaaataaaat agacattgaa caaattcggt ttgatagaaa
     8101 aaatcgtttg ttggatcagt agagacaaga gttggagaaa gtaatttggt tcattgattt
     8161 ctatttcttt ttttagatcg ttcagtaaat tctctttctg ttgatacaag tgtgcatatt
     8221 gctgatcata tgtttctaaa aaacgatggt attgacggtc caatgcttca atcaatatcg
     8281 gcgattcatg atattttaat atgaggagag ccaagttatc agtcagagat gtttttttta
     8341 gttgatcaag agacagctct gaagctagtt cctctacaaa cgtttcgagg acctctaaaa
     8401 atggagaatg atgcaggcag cgagcggttt cttgtccata gttattgttg taattccaac
     8461 tttccagaaa gcagaaagaa gcaaaagaaa gctcagtgtc tttaagataa ataccttccg
     8521 ttaaatacaa ttttttcaat gcagagtcat acagggagga gagtgtgctg ttggtatgga
     8581 tagaagaatc taatcggcaa ttcgctttta tacggatcgt attgataaag aaccaacatg
     8641 ttccgaaaat ctcttctgtt tcaaccgcta gttcagtttg actgaggttt cttaaaaatt
     8701 gaaaataatg ggattcatgg atcgaataat cctcgaaaaa atagttgtta tgagtgaaag
     8761 gaatcagcaa tcgatgataa aaagtacgaa tcgcacgctc ttttccaaga atacaagctg
     8821 tatatttcgt caaatcgatc tgcaagtcgt aacttgctaa acgctgattg attttagtca
     8881 catgtctttt caatgtccca taagaaagcc catggtcggc tagaaaatca gggacagcaa
     8941 ctttttttgt aaaaagcaga tttttcagca gttcgacact gactgattgt ttgaaaaaaa
     9001 tctcccaaag gtcatttgtt aataaatttt gctggttttg caaactgatt cctgcttgat
     9061 cgctgatgat gtcccagcct tcgggcagct gagagcgaat agattgaagg tcagaaaaaa
     9121 tcgttcgttc ggttgtccct atgatttcag ctaattcatt tacggttatc tttgtatgat
     9181 tcagcaattg ttctactaaa gaaacatgac gtcgaatatc tttttcggta atgatttttt
     9241 tcattagtga atacatcaat cagactccct tctattctaa gaatacggag aaaataaacg
     9301 gtggtcaagt ttcaactgat ttttcaatca ttgtttattt ttctggagca tttactgatg
     9361 ataagaattt catcatgaac ttgaaaaatg ataccactaa aatcaaaaaa taggtgggca
     9421 aacaataata aacattttaa tagtagatgg tagcaagacc aacacaggcg gattgaacat
     9481 ttaagagttg ataaaaaaga aaaatatcga agtatttgat tagcaactac ttgtattcat
     9541 ttttctattc ttacgtggta tctttgaata aagcgaatca ataagaaaga gatggaagca
     9601 gatgctaacg aaaaaagaac taatcgatca catcaatgaa acatacaatt tgtctccaga
     9661 atatattttt aaaaaatttc ctgattattg cgtattcaga catcaaaaaa gcaaaaaatg
     9721 gtttggggtc ctgatgtctg ttccagaaga aaaaatctat gatgatgcaa gtgaccatag
     9781 gatcatcaat attcttgatt tgaaggtcga tcctgaactt ggcggaattt tgcgaggaaa
     9841 agaggggtat tttccagcct atcacatgaa taaagaacat tggatctcta ttgatctacg
     9901 taaaatagaa aagctagatg aactagcaga tttgattgat ggaagtttta aatcaacgca
     9961 atgaatgtct aggacaatag agaagaggtt gtgacagaaa tgtttagctt caagaaataa
    10021 gaaggaattt acgaaaattg cttctcaaat ttttgggaaa tttcggctta ttttctaaga
    10081 aattacttct ggaacaccgt ttgttcgaaa aaagagctgc gacaagaatt ttgtcccagc
    10141 tctttttttt ttattttcct aaataaaatt cccgcatcgt ttttgcaatc attttgatca
    10201 aaggatgatc ttctgtgaag ctagaagata aataaattgt tcgtgtcaag ggcaaatctt
    10261 tgatttcccg caagacgaaa ggatcatttt tatcgtaaga ccaagaaact tttgggaaaa
    10321 agctaacagc taatccttgc ttgacaatag aacgtaatgt cgcaggatca tctgtcgtcg
    10381 aactgatcgt caattcgata cttttcgctt caagtgctgc atccagtgta tgacggaagg
    10441 cgttgctctt tgacagcatc aagatggggg tgcgttggat atcttctaat tgcagatgtt
    10501 cttttttagc aagaggatgt ttcttaggaa tggcgacata aacaggttct ttcatgatag
    10561 gaatattggt catgccaggc ttcatctccg aagtgatgta cagtacagga tcggtaatat
    10621 catctggtaa atgatgttgc atgatcgtta attggatatc aggatgcttt tccataaaaa
    10681 ttcggaccaa gttcggaata gaaacagagg ctacctctaa atacaatatg acggaagtaa
    10741 cgatcttctc attgaccgaa gacaataatt tgattccttc ttctaatgtg tctaatgtct
    10801 tgactgcata tggataaaat tcttctgcat aaacggtcgt ctcaatgttt ctgccgctat
    10861 ggtaaaacaa agggacacct aattcgtttt ccaaatgttt gatcgcacgg ctcaaggcag
    10921 gttgagaaat aaataattct ttggctgctt gggtaacatt ttttgtttcg aatacttttt
    10981 taaaatactg cagatgaagc aatttcataa ttatccatcc catccatacc tttttgttat
    11041 ggttttttct ttttttggca ttttttatta tagcagaaat tgactaagat agcagtggaa
    11101 aaagtaagtg ctgaggaggc gaggctcatg agtacaggtt tcttatttcc tggacaaggc
    11161 agtcagaagt tagggatgtt agcggatatc cctgaaatgt acatggaacg tttagaaaat
    11221 gtgacggggt atcggttagt tgataaagaa gaaaactatc aagatacggt ttttatccag
    11281 ctagcattac tgacaaaagc agtattttat ttagatgaaa tggaaaaaaa cggtattcag
    11341 cctgatattg cagcaggaca ttcgattggt gctttttgtg cagctgttgc ttgtcgatct
    11401 ttgagatttg agaatgcagc ggaactagtc tatcaccgcg cagtcttaat gaaagaagct
    11461 tatccaagcg gttatgcaat gggcgtagta gtaggtgtga cgagaagcga agctgaggaa
    11521 attgtagcaa aaacatttga tcctgaccat ccagtttatc tgtcaaatga aaattgtccg
    11581 atgcaacata cgttatctgg gagtatcgaa ggtttatccg ttacgttggc agaagccaaa
    11641 cggcaccaag cgcaaacagc gaagctgctg aaagtccctg tgccatctca ctgtctcttg
    11701 atgaatgata cggtcaagaa attcaagccg tatatgcagt ctgtccatct atctgatgct
    11761 agatgtttgt atataaaaaa tacggatgga agagccactc aggacgcaga agagattcgt
    11821 aaagatttgt tggaaaacct agctttccct gtgcagtgga accaaatgat ggacttggca
    11881 aaagaattag gaatggatct gacgattgaa tttccccccg gcaatacttt gacaaagctg
    11941 attcatacaa aatttggtgt agataaaggg atccgtacga tcaatctaga ccaacatggt
    12001 attgatgacg ccacgtttct atatcaaaaa tggagatgac taattatgga aaaaccaaac
    12061 cgcaactggg cttctaaacg cgaaaaaaaa gcgcagcgat tggaaaaaat cagcaacttg
    12121 ctcaatggca agtatgtaga aactgaaaaa atcgtagaag taatggaaaa actgattgct
    12181 cccggcgaca aagttgtatt agaaggcgac aatcaaaaac aagcaagttt tctttcagaa
    12241 agtcttgaaa aagtagatgc agaaaaagtg aatgatctgc atttgatcat gtctagtatc
    12301 tcacgaccag aacatttgaa tatttttgaa aaaggaatcg caagcaagat cgatttctct
    12361 tatgctggtg cacaaagctt gcgtgtcgcc caaatgattg aagacggcac aatgaaatta
    12421 ggtgatatcc atacttattt ggaactttat ggacgcttat tcatcgattt gattccaaat
    12481 gttgttttag tagctgcaga taaagcagat aaagacggca acttgtacac aggtttcaat
    12541 acggaagaaa caccaacaat catcgaagct gcagctttca aagatggtat cgtgatcgtt
    12601 caagttaacg aagttgtcga ttctttacca cgtgtagata tccctggtga ctgggtagac
    12661 gttgtcgtga aagcagataa accttatcaa ttagaagctt tattcacacg agatcctcaa
    12721 aacatcacag aattgcaaat tctaatggca atgatggcaa tcaaaggaat ctacgcagaa
    12781 cacggcgtac aatctttgaa tcacggtatt ggattcaata cagcagctat cgaattgtta
    12841 ctgccaactt atggtgaatc actagggtta aaagggaaaa tcgcgaagaa ctgggtattg
    12901 aacccacatc caacgatgat tccagccatc gaatcaggtt gggttgaaag catccatagt
    12961 tttggtggcg aagttggtat ggaaaaatat attgctgctc gcccagatgt cttttttgtc
    13021 ggtaaagacg gcacgatgcg cagtaaccga acattatcac aagctgcagg acaatatgcc
    13081 atcgatatgt ttatcggttc aaccttgcaa ttagattacg aaggaaactc ttctaccgta
    13141 acaaaaggac gtttatctgg ttttggcggt gcgcctaaca tgggacataa tcctggcggt
    13201 cgtcgtcatt ctacaccagc atggcaatca cttcgcgaca atgatgatcc attagggaaa
    13261 ggccaaaaat tagttgttca aatggttgaa acatatggtt caaataaaaa accagtcttt
    13321 gtcgaagaat tagatgcact agctgttcaa aaacaagcag gcttagccaa tgcaccagtg
    13381 atgatttata gcgaagatac tacacatatc gtgacagaag aaggaattgc ttatttgtac
    13441 aaagcaaaaa caaaagaaga acgacaagca gcaattgcag caattggcgg tgttacacct
    13501 cttggaatga ctagcacgaa agaatcacta gatagcttgc gtgcagccgg catcgtgaaa
    13561 ttaccagaag atctaggcat caaacgtagt gaagcgaaac gttcattatt agctgcacaa
    13621 accatcgatg atttgatcga atggtcagat ggcctgtatg aaccaccaat gaaattcaga
    13681 acttggtcat aaaattggcg agaaaaacct tttgtcgcca tttacccaga tggaagaaga
    13741 aagacgaata agcagaacgt gtatgctctg ttttacaaaa aggggagaga tccatggaac
    13801 aattagctta taaatatcca acgaaacagc cgatcaataa gaaaatccat gtaggagtgg
    13861 tcggttcagg agatttggaa atcttgatgt ttccaacaga aaaaacagaa tctactgtaa
    13921 aaatttgtac aggaagcgat ggcttcggcg aagtatggaa caatgtatta acgagatttt
    13981 ttgatcgtta tccaatttca gcagatatcg tgatcaatga cttcggtgct acaccaggag
    14041 ttgtgtacct acgattgacg caagcaatgg aggaactttc agatgaaaaa tagttttgta
    14101 gaattaaacg ccagagaacg agtgaacgct ctacttgaca atggacgtga attgattgat
    14161 ccattcgatc aaatcatcgc gccaagttta ccagcacaag gaatcgtccc tgaaagtgat
    14221 gacgggatcg ttgtcagccg tggcacgatc aatggcaaac aagcagtagt aatcgctatg
    14281 gaaggaaaat tccaaggtgg cggtatcggt gaagtttccg gtgcaaaaat cgttgctgca
    14341 cttgaacatg tgttagaaga aaataaaaaa ggaaatgaga tctttccagt cattgtacta
    14401 gatacaggtg gtgttcgttt gcaagaagca aactacggac tgttatctat ttcagaaatc
    14461 ggaaatgtaa tcattgctct aaaacaatat gtaccagtca tcggtttgat tccaggacgt
    14521 gtaggatcat ttggcggtat gtcgattact tcagcattaa tgagttattt aatcgcaaca
    14581 aaaaaagcga gagtcggttt gaatggacca gaagttattg aacaagaagc aggcgttcga
    14641 gaatttgatt ctagtgacaa agacttgatt tggaatacat taggatctcg tcaacgggta
    14701 gaatcaaaaa tcattgatga attagtgact gatagtgtag aagcaatcaa agctgctgtc
    14761 atcgcagcaa tggacgaacg aaaagacacg catcgcagtg aaagctcagc attttattta
    14821 tcattacttg attctttaga tctttcaaaa ccaatggcca ttgacgatta caatgaagca
    14881 attgctaacc atcaagcaaa atcaattgac ttaccagaag tagcagaagg aaatgaacca
    14941 gctgcagcaa gtgttggtta cgattggttt gcggcactaa ctggcatgaa aaatccaact
    15001 tcttctatta gtactgtgta ttatggtaaa tcaagtaaat tcatagaaga agccatcgtg
    15061 acatcaatcg tacctgatcc taaaaacccg atgtaccgtg tacgaaacgg tgaagttggt
    15121 ttagttgaag gtttccgcat gggacaaatt ttggatcacg tttacgaaga agataaagac
    15181 aaaaaagtga aacggccaat catcgttgtg atcgatgtac caagccaagc atacggctat
    15241 aacgaagaat tgatcggtat ccatgtggct ttggctaata gtgctgctgc ttatgcaaaa
    15301 ctccgtcaag ctggacatcc agttattgga ttgattgcag gaaatgctat ttcaggtgca
    15361 tttttagcac atggattaca atcaagccgc ttgattgctt taaacaataa agaaattacc
    15421 gtccaagcga tgtctaaaga aagtgctgca cgtgtgacaa aacgttcggt tgctgaaatc
    15481 gaagcagctg ctgaaaaagt tccagctatc gcttacgata tcaataatta tacaaaacta
    15541 ggagcgatct atcatttcct agaagatatc accgaccaag ctgcttcaga acaaaatgtc
    15601 agcaaagtca tcgaagcaat caatgaagcc attcaagatg tccgcaatac aaatgacacg
    15661 atgctgacaa atcgatacac aaacgaaaca gccaaaacat tcggacgtgt cgaaacaaat
    15721 aaagtccgtg ccaaaatgga cgaagaatgg gatgcataaa aaatgattca agcacatgat
    15781 attgtccttt ttgataaaca tgaaatagaa gggttcacct tgcctgaatg ggtcgttcta
    15841 actgaaaaga tgttcggaac agttcgtcgt gcgtcgacgg atcacgcaga aatactaccg
    15901 attggtttac gtggacctgc ccgcaatcag agatggtcaa cagaaatctc aaaacatgcc
    15961 gtcaaacaag tcattcatcc gtgggacatc atcgaacaag aatcgtatag acaagagaaa
    16021 atcatggatt atcctgttta tcagcaattt catgctgcta gaaacatatt atcaggatgt
    16081 aaatggggaa tcggcggcag cttaggattt gaattatcga ctgggattcc ggcagtaaaa
    16141 gaaaccagtg attttgattt gctgctgtac gcaaattcac cgattgaatt gccaattcag
    16201 gtaattcaat cgcatcctgc tttttttgag caattcgata cccaagtagt gactcggcaa
    16261 ggcggttttt cactaaaaga gtatttgaga gagccagaga aaaaaattct attgaaaaca
    16321 gtcgaaggac ctaaattaac caaagaactt tggtaataaa gaaaaagaga gagaaagaga
    16381 tgtttgaaaa caaaaaaatc aaaatcaacg taagtgactt ccagaaagta tcagctgtaa
    16441 cggctgttat gatgcaaacg atattgagct ttgctttggc aaacagccac agcgcaacaa
    16501 atacagcttt tcttggtacc ttttacgtca tcgttaaata cacagcacca atgtttattt
    16561 ttgcaattgt ttataatatg gtaaaaacaa gtcagcattt gtcttattgg gaatttttga
    16621 aagaaaagtt ttttgaacta gtcgttccgt atcttctatg gacgacggct tatctttggc
    16681 tatttccagc agtccaacaa caaactccgt atacggatac tgcaagcttt ttactgaaat
    16741 atgtcacagg tgatggagcg cctcatttat ggtataccgt tatgatgctg cagatccaat
    16801 tgttcatgcc gttttttgtt tggctcggtt acaaagtatt tgctaataaa aaatatgtat
    16861 ggccagtatt gattggttca acgattttat atgtcgcatg gtatctgttc tatcaaacac
    16921 aagtattgac tgggccttat catgacagct ggtacttatt agatcggttt gttgctagtt
    16981 tcatgattta tggggtagcc gcatttgttt atcacgaaaa agtctatcaa tatttggaca
    17041 gagtacgtta tcttttctta cctgttgcct tagttatcgc gttcttttct gtccgttcat
    17101 tgctcgcaca tccaggagac ttgagctttg cgaatgcacc ttacttgaat acgatccaaa
    17161 gtttatacag tttagtcatc atctttgctg tcttcatagg cgcttccaaa atgattgtaa
    17221 acgattcgcc aaaattgcca ttgttcaaat ggttgtctgt ttatgcttat cgtacgtatt
    17281 tagccaatgt atttgttttc caagtactgt tattattgtt taaagatagt tggctgcagt
    17341 taccttcagg agtcatgatt cttgtagctt atctaatgac ggcaagttgt gcatttgctt
    17401 taagctggtt acttcatatc atttgggtag ctatcaaaaa aggtttcagc aagtaatagt
    17461 aggaagaagg agtgagtgtc atgataaatg acgaacgaaa aaaagcagtg actcagcaac
    17521 tcgctgcgtt ggtagaggaa gcattgatcg cagaagtaac attaagtcca aaacctggat
    17581 tagtggatgc actggatgcg ggtgcacatt ccgatatgga ctatgcttta tttcttaagt
    17641 ctgctaagac gctaactcct ttttttgaag aaatggctca aatagcatgg catcatgcta
    17701 ttgatcagga gttaagagaa agaattgctg agattgggcg aaaagcggaa aaggcaatgc
    17761 tgattgctac aaatggcatc aatacccata aaggagcgat ttgggcaatg gggctattga
    17821 tcagtgtcat tgcacaaaaa atcagtatgg aacagccttt ttctttatcg aaaatacttg
    17881 atgatgtcag caagcttgct gcttttcctg atacaaaata tcgtcataaa aaaaccactc
    17941 atggtcatag ggtgaagcaa aaatatggcg taaatggtgc atatgaagaa gcgatattag
    18001 ggtatccgca tatcaggctt gcaattcaag cagctaagga gctagataat gattcatatg
    18061 tacagaaaca aatgcatatg ttgttagttt tgatgagttc gctagatgac acatgcgtat
    18121 tgcatcgcag tgatcagaaa acgttagtgg aaatgcaaaa acttgctcat gctgctagtc
    18181 aaagtgcttt accaaatcgt ccatttacag cactgttgca cttttgtcag gaaaaccaaa
    18241 tctctcctgg aggaagcgca gatctcttgt ctgcaagctt gttcatgctg aagatagagg
    18301 aattatggac tgccgacaca tacatttgtc cgatgttact caatgactaa ataagacaaa
    18361 attgctaaaa aaacagttta tgcatctggt tattgactct tccttttagc aaggctatca
    18421 taaaaatagt tttttgtaat aaaagataac gaggaagaca atgaaaaaaa tcaattattt
    18481 agcaatcatt ttgttagtgg gcctatgttt gtttgctctg ccgacccaag gattcgctgt
    18541 cgcgggtggt catggtggag gtacatctag cggtcgaagt tcgggcagag gttcatttgg
    18601 tggtcgttca tatgggggca gttattttgg cagtcgttct tacggcagag attcttatgg
    18661 gtattatggt ccaggtacag gtccttctag ttatgagcaa acgggatttc ttgcattagc
    18721 tgttttgtca gtaggagggg tttctgcttt ttctatcgtc aagaataaaa gacaaaagaa
    18781 aaggctgaaa gcggagatat tgtcccgcat gtcaggaacg gctaaagaaa agaaagcaca
    18841 gtttctggaa atccaaaaga tattcatgaa gatccaatcc gcttgggaag aagaaactcc
    18901 tgaaaaagcc agaagctgtt atacagacaa gctgtttgcc gaacatcaga aagttctgca
    18961 aaaaaacaaa gcagacggtg taagaaatca tactaaaaaa ataactattc aggaagtagg
    19021 aaattatcga cagatccacg aaaatagttt tgccatccga attgatttca gatgctgtga
    19081 ttattcagtc gatcgaaaag atgggcgatt gatctcagga agtaagacac aacgacagta
    19141 tttttctcag atatggtatt ttgatttttc tgagaaagaa aaaagatggc aagccgattt
    19201 tattcagccg attatgctgg attgacagga aatatttatg aacggagttg tgacaaaagc
    19261 cttgtcgcag ctcctttttc tgaataaacg gtgtgccaga gatagcctat cggcaataag
    19321 gcacgatttt gtaaaatatg ggaagcgatt tttcaaaatc gcttcttatc gctcaaggct
    19381 gaacatctct gtcacaacct ctccactaaa tggtgtttaa aagcttgcat ctgcgataat
    19441 aagaacaacc aaacgaaaaa taggaagcac tattttagtt tagttgttct tattacttga
    19501 tgcaggacag ctttttcaca acctcttttt tgtatagatg ggcaaaattt ttgtaataat
    19561 aggcgtatgt ctttttcgtt gaggagatga aaaagcatat ggcttatttt ttgtttgctt
    19621 tgcttacttt ctttccactt caagtggcag aaatatctac tttttattat gtaagcggaa
    19681 ccatatactt gaaatcagaa ggagatcatt tctatgaaaa caagaacaat tgaaatactt
    19741 cacagaatca tgacagccca atatccagta aaagcaaaac atctagcaag tgagttcaat
    19801 gtcagtcagc ggacgatccg tcaggaagtt cttgaagcga atatctggtt gagcagtaaa
    19861 aagcttccgg agatccgtac aattcgcaac aaaggttttt tgttgaagct ggaaaaggaa
    19921 aagcaagctc gattagaaac tgaaataaaa gatgtgaaag acggcttttt gaatcggcgc
    19981 gaacgtattt ttgatctagt tttagccgtt gcttacagcc gaatcccgat ccatctaaac
    20041 aaaaaagaag aagaatttca aatctctaaa agtacattag atgaagatat gagacggtta
    20101 cgagcaacac tggtcagtta tgggatagag atcgtcagtt atggaaagca aggactggtt
    20161 tataaaggtt cagaacgatc tatccgtacg atgatctatg attttatcaa tcagaatctg
    20221 ggaatgaccg acttctttat gaatcaagat gcacaattaa caccggctca aaaaatcttt
    20281 ctccattatt ttcctttggt ggaaatcaaa aaaatacagg agatctattc caataagttg
    20341 ttgaaacaag aagatgatat ttataaaaaa caaatcctgc tttttacatt gatctggatc
    20401 cagcgaatca ggaaacatga atttatctct gccattaatt ggaaaaatac agaaataaaa
    20461 gacagtagct tttctgcatt tattgaccac atgatcgagg aatttcaatt ggacaatgtc
    20521 ccgcaagtag aaagaaatta tcttcgtttc actattgaga cgttcaatgc tagagatatc
    20581 tccaattcaa tcgagtgggt acaagctcaa ttactaacga tccaattgat ccaatttgta
    20641 gagaacgaga cacacgtacc tttccatcta aaggaagaag ctttattcga aaatctctat
    20701 aagcacatgg ctgcactgat tgtaagaatc aagaatcata tccaagtcat gaatccattg
    20761 aaagaaaaca tccgaacaaa ttatttccca atctaccgcg cagtcactca ttttgttcct
    20821 acgatcgaag aagtaattgg tggcaagata ctggaggacg agatagcttt tcttgtcatc
    20881 cacttttcga cgatcgccag tgtaatcaag cgagacttga attatgttta taaatcagtc
    20941 gttgtctgta accatgggat ggctactgga aatctgttag cagagaatct gaaagaaaag
    21001 tttcctcaga tcgagattac tgcagtttta agttcaaaag aaatcgaatt agtagataaa
    21061 ttggatgtca atctggtttt cagcaccttt caattgaatt atcgaaataa gccgctttta
    21121 gttgtcgatc cgattttaac tgagacgaat atcttggtta tagaagactt tttgaaaaga
    21181 aatgaagaac tacagcggtt agagcctaac agtcatgaat cgacggagtt attcactcaa
    21241 ataatagatg tcatgaaaga aagtggtggc cgagtagatc gaatcatcta tggcaagtta
    21301 gaaaaattat tcgaaacaaa caatttagaa atcaacaaaa gggagatcca gcctatgtta
    21361 aaagatattt tgacggacag caatatcatg atcaaagaaa aagccactac ttgggaggaa
    21421 tcgattgaac tagctgctag accattggtt aaagaaaaca ttgtcgaaga gcgctatgtc
    21481 gaagcgatga ttcgtgcagt gaatgattac ggaccgtata tcgtgattgg caaacacatg
    21541 gcactagccc atgcccgacc agaagatggg gtcaataaat taggagtcag cgtagctacg
    21601 attgagcaac caatcgattt cggtaatcct gaaatggatc cagtaaagat tattttttgt
    21661 ttggcagcag tagactccta ctcgcatctg aacatcatga aagaactaat tgaattgatc
    21721 aacaacgaag agaagttgga tcgattgatt gaatgcacaa atatcgaaga tttcaaacaa
    21781 ctgctttttt cagtagttga gaaataataa atgatacggg ggagaacaaa gatgaacaaa
    21841 ttgaacattt tattcgtatg cggtgcaggt ttaggaagta gttttgcagc acagatgtct
    21901 gctgaggatg ttttgaacaa attaggagta gaagccaaac tggaccattg tgatatttct
    21961 tccgcagttt ccatgaaacc agatgtgatc atcacagcag aaaattttcg ttcgcagttc
    22021 gaaaagttcg cagttgatcc taaaacggcg atgatctatt tgaaaaatat tgtttcaaaa
    22081 aatgaaattg aagaaaaaat caccccagtt ctgcaagata agggagttat ctaaatacaa
    22141 aaagaaaaga gggtaaaaa
//
LOCUS       DAYYBG010000044        21977 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000044 DAYYBG010000000
VERSION     DAYYBG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.044"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1211
                     /locus_tag="ACHAUN_001577"
     CDS             207..1211
                     /locus_tag="ACHAUN_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460281.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1212..2156
                     /locus_tag="ACHAUN_001578"
     CDS             1212..2156
                     /locus_tag="ACHAUN_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460282.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2156..3118
                     /gene="opp4B"
                     /locus_tag="ACHAUN_001579"
     CDS             2156..3118
                     /gene="opp4B"
                     /locus_tag="ACHAUN_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8460283.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3145..4059
                     /locus_tag="ACHAUN_001580"
     CDS             3145..4059
                     /locus_tag="ACHAUN_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8460284.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4085..5866
                     /locus_tag="ACHAUN_001581"
     CDS             4085..5866
                     /locus_tag="ACHAUN_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8460285.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6214..6900
                     /gene="rnc"
                     /locus_tag="ACHAUN_001582"
     CDS             6214..6900
                     /gene="rnc"
                     /locus_tag="ACHAUN_001582"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8460286.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6920..10501
                     /gene="smc"
                     /locus_tag="ACHAUN_001583"
     CDS             6920..10501
                     /gene="smc"
                     /locus_tag="ACHAUN_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8460287.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10510..11319
                     /locus_tag="ACHAUN_001584"
     CDS             10510..11319
                     /locus_tag="ACHAUN_001584"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8460288.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11330..12328
                     /gene="ftsY"
                     /locus_tag="ACHAUN_001585"
     CDS             11330..12328
                     /gene="ftsY"
                     /locus_tag="ACHAUN_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8460289.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12515..14782
                     /gene="gshAB"
                     /locus_tag="ACHAUN_001586"
     CDS             12515..14782
                     /gene="gshAB"
                     /locus_tag="ACHAUN_001586"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8460290.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14871..15323)
                     /locus_tag="ACHAUN_001587"
     CDS             complement(14871..15323)
                     /locus_tag="ACHAUN_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8460291.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15473..16423
                     /locus_tag="ACHAUN_001588"
     CDS             15473..16423
                     /locus_tag="ACHAUN_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8460292.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16416..17375
                     /locus_tag="ACHAUN_001589"
     CDS             16416..17375
                     /locus_tag="ACHAUN_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8460293.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17372..18127
                     /locus_tag="ACHAUN_001590"
     CDS             17372..18127
                     /locus_tag="ACHAUN_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460294.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18150..19103
                     /locus_tag="ACHAUN_001591"
     CDS             18150..19103
                     /locus_tag="ACHAUN_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8460295.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19747..20043)
                     /locus_tag="ACHAUN_001592"
     CDS             complement(19747..20043)
                     /locus_tag="ACHAUN_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8460296.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20398..20754
                     /locus_tag="ACHAUN_001593"
     CDS             20398..20754
                     /locus_tag="ACHAUN_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8460297.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20764..21663
                     /locus_tag="ACHAUN_001594"
     CDS             20764..21663
                     /locus_tag="ACHAUN_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8460298.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         7272 a         3872 c         4675 g         6158 t
ORIGIN      
        1 atctttttga cacgtaaaag tcatttttct gataattctg gcgataaaca gaaattatgt
       61 tgcaaagtaa agcggttttc tatataatta ttaataattc attacacaaa gataagaata
      121 acctttgtta taaaacgatt tattctgtgt aatgtccttt tttattaaga aaaacttcca
      181 tttaggaagc agagaggggt attcgattgt ccgataacca attattagaa gttcagaacc
      241 tacatacagg attccgactc aaagatgact attacgatgc agtagatgac gtttcattta
      301 ctttagacaa aaacgagatt ttagcaatcg ttggggaatc tgggtgcggg aaaagtacct
      361 tggctactac gattatggga ttacttgatc ctaataacac aaaaattaca ggtgagattt
      421 tatataacga tctgaattta atcgacctga acgaaacgct ctataataaa atccgcggaa
      481 atgatatcgg aatgattttc caagatccgt tgtctgcatt aaatcctctc atgagaatcg
      541 aagaccagat caaagaagga ctttcctatc acacaaagat gaatgcagaa cagcgtcaag
      601 ctcgtgcact agaattgcta gaacaagtag ggattcctaa tcctgcgaga gttggacgcc
      661 aatatcccca tgaactatca ggcggtatga gacaacgtgt catcatcgca atcgcaatcg
      721 cctgcaaacc tccaatcgtc attgcagatg aaccgacgac agccctagat gtaaccatcc
      781 aagcacagat tcttgattta ctgaaagacc ttcaggaaga aacaaaaaca gggatcatct
      841 tgatcactca tgacttagga gtcgtagcag aaatggctga tcgagtggca gtgatgtatg
      901 gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt tgatcatcct aagcatcctt
      961 atacacgctc tttactgaac tctataccac aagaaggaaa tcatgatgca gaactccatg
     1021 tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg taaaggttgt cggtttgcac
     1081 cacgtattcc atggatacca gcaagcgatc atgaagaaga gcctatcctt catgaagtcg
     1141 caccgaacca cttggtacgt tgctcctgct ataagcattt ccactttaga gacgaaaaag
     1201 gagaggtgta gatgacagaa ttaatcacaa tcaaagacct gaaagttcat taccctattc
     1261 gcagtggatt cttcaatcga gtaacggatc atgtatatgc tgtagatggt gtggatttta
     1321 ttatcgaaaa aggaaagaca tatggtttag tgggagagtc tggctctgga aaatctacta
     1381 cgggaaaagc cgttgtggga ttagaaaagg taacagccgg acaaatcatg tatgaaggaa
     1441 aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta taacaaggat gtgcagatga
     1501 tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg agtgttggat atcattgctg
     1561 aacccatccg aaattttgaa cggttaagcg atcaagaaga aaagaaaaag gtaaaaggac
     1621 tgctagatat cgttgggatg ccggaagatg cgctatataa atatcctcat gaattttcag
     1681 gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc taccaatcca aaactgatcg
     1741 tagccgatga acctgtatct gctctggatc tatctgttca agcgcaagta ttgaatttca
     1801 tgaaacgaat ccagcaagaa tacggcctca gctatttatt catttcacat gatttaggtg
     1861 tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa aggtcgattt gtagaaatcg
     1921 gtacaagaga agacatttat aatgacccgc gacatatcta tacgaaacgt ctgctttcag
     1981 ctattccgca aatcgatgta gaaaataggg aaagccacaa agaaaatcga cgaagagtag
     2041 aacaagagta tatccaaaac caaaaagaat actatgatgc gactggtcga gtctacgatt
     2101 tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga tggaggtgca agctaatgtg
     2161 gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa gttattattt taagtgttct
     2221 gatcttctta ttagcccaag cgatgccggg ggatccattt accggtttga tcaatccaaa
     2281 tcaggatccg gcagtgatcg aacagatgcg acaagcagct ggcttgaatg acccatggta
     2341 tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc gattttggtc aaagtttctt
     2401 atacaaacgc tctgttgctg acttgatcgg cgaacggatc atcaacactt tatatctatc
     2461 gatcttgaca gttgtcctta cttatttgat tgctgtgcct ctggggatgc ttgctggccg
     2521 gtatcaaaat tcaatcttgg ataagattgt cgtaatctac aacttcttta gttttgctgt
     2581 tcctttattt atcttcggct tgatcatgct atttgttttt ggttatcgtt taggctggtt
     2641 cccaacaagt ggatcacaaa cactaggttt gcaaggtgga tttatcactc aatggataga
     2701 ccgtttgaaa tatattctgt taccatcaat tacccaagca tttttggcga ctgccgtcac
     2761 aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg ctcgattttg tcagaacagc
     2821 ccgttccaaa ggggtaccga cgaataaagt attcagtcga catattttcc gaaatgcagc
     2881 gttgccgatg gcttctcaaa tggggtatga aatcacttcg ttgattgctg gttctgtagt
     2941 tatcgaaaag atttttggtt atccgggagt cggaaaacta tttattgatt ccatcggcca
     3001 acgggactat accgtgatca catccttagt attgatttta ggaatcgcga cgctagtggg
     3061 gacattactt tcagatatca tcatgagtat cgttgacccg cgtattcgga ttcaataaaa
     3121 ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca atacagcagt tcaagaaagt
     3181 attcctccta tggggatacg aatgattgct cgagaattta aaaaagataa aattgctatt
     3241 ttttcattag gtttgctagt ggttttatta ttagtgatct ttatcggtgc gatgctgacc
     3301 gatcaagaca aagtgatgta tgttagtatc ttcgacaaat acgctgcacc aggaacagtt
     3361 tcgacacaag gacaaaagtt cattctggga gcagatgaag gcggacgtga tgtattagga
     3421 caattgatca taggtgctag aaactctgtt acgatcggat ttgcgatcac gatccttact
     3481 tcgatcatcg gggttggatt agggatcatc tctggctact acggagggat cattgacaac
     3541 atcttgatgc ggatcgttga ttttatcatg attctaccga tcatgctgat cattatcgtc
     3601 ttcgtatcga ttatttccag atacaatgtc tggtccttta tattcatcat gagtgcattt
     3661 tattgggtag ctaaagcccg attattccga agcaagacat tatctgaagc aagaagagat
     3721 tatgtcagtg cctcaaaaac aatgggaacc aatgatttta aaatcatgtt ccgcgaaatc
     3781 atgccgaatc taagttcatt aatcattact aatttgacga tgaactttgc ggcaaacatc
     3841 ggtatcgaga caactttgac tttcttaggt ttcggcttgc cggcaaatgt acctagttta
     3901 ggtacattga tcggttatgc aagtaacgga gaagtcttag tgaatcggca atggatctgg
     3961 ttacccgcat caatccttat tttagttttg atgttgagta tcaattatgt tggacaagca
     4021 ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt atagaagcga agggggaaat
     4081 aggaatgaag aaaacgattt ttggatttgc agcgctgatg tctgctgtcg ttcttgcagc
     4141 atgcgggggc ggaggtacag atacctctgg aaacaactct ggaggcaacg gcaataacaa
     4201 agaggaatta acttttccaa ttgcaacaaa aaataatgat gatgcaatcg aagacggcga
     4261 attagaagct gcggtcgcaa cggacagtca gtttaaagga ttgttccagt gggagtttta
     4321 ccaagattca tatgatgcag cttttatggc tccttctcat gaaccgctat ttgcaaacga
     4381 tgacgatttc agaatcacag acgacggagc tgcaacgctt gatctagatg aagatacgaa
     4441 aaaagcgaca atcaaagtaa aagacaacgt caaatggtca gatggtgaag atgtaacggc
     4501 ggacgatgtg attttccctt acgaaatcat tgggaataaa gattacactg gtatccgtta
     4561 tgacgacact ttccgtaaca tcgtaggtat ggaagaatat catgatggaa gtgccgacac
     4621 aatctccgga atcaaaaaaa tagacgacaa aacagttgaa atcgaatata aagaagtaaa
     4681 cccaagcatg cttcaatcgg gtggtggtat ctggtcatat gcagcaccaa aacatacgtt
     4741 agaaggtgta gcaatcaaag atatggaatc aagcgatcaa gtacgtaaaa atccagtgac
     4801 ttttggccct tattacatga gtaatatcgt tgcaggagaa tcagtagaat acttgccaaa
     4861 cgagcactac tggaatggta cgcctaaatt gaaaaagatc acgatgaaat ctgtaccgat
     4921 ggcaagtgca actgaagcat tgaactctaa attatatgat atgattttcc aaatgccaac
     4981 agatacttac gatacctata aagaaattgc tggctataca aacttaggcc gtgaacaaac
     5041 ttcttatact tatctaggat tcaagttagg taaatggaat gcagatacaa agagtgtgga
     5101 atacaatcca gattcaaaaa tggctgataa atcattacgt caagcaatgg gctatgcatt
     5161 agataataat gcagtaggtg aacgtttcta tgcaggtttg agaagcaatg cgacttcatt
     5221 gattcctcca gtattccagt cataccatga tgatgagatg aaaggattca cacaagatct
     5281 agacaaagcg aaaaaactat tagatgatgc tggctacaaa gatgtggatg acgatggttt
     5341 aagagaagac aaagatggaa ataaactaac aatcaacttt gcttcgatgt ccggtgggga
     5401 aacagcgcaa ccattggcag attactatat ccaacaatgg aaagaaattg gtttggatgt
     5461 ccaatacaca acaggtcgtc tgatcgaatt caattctttc tatgataaat tagaaaacga
     5521 tgatcctgaa attgatgttt accaagctgc ttggaataca ggaactgatc caaatccaag
     5581 tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt tatgaatcag aagaaaatac
     5641 aaaattgatc aacgatatca gttcaagcaa agcatttgat gaagagtacc aaaaagaagc
     5701 atatcaaaaa tggcaacaat atgcatttga agaagcattt gcgattccaa cgttgttccg
     5761 taatgaagta ttgccagtca acgaccgtgt caaagactgg gattggtctt atgatgtacc
     5821 aaacccatgg gcaaatgttt ctgttacagc agattcacgt tcataatcac taaaaatgac
     5881 cgaaaaaggg cgatgtcctt tttcggtttt ttgatatttt tctgtttatt tctctaatat
     5941 tcaggtcaag gcgctgattt catgacttct ccaataaacg gtgttcaaag tccagtttct
     6001 cggtattaag tcaaatttat gcaaaaatat ggaaagccat tttcacaaaa ttttttcttc
     6061 atactcgaaa ctaaacgaat ttttcacaac ttctcgtcaa tcactaggat tattaaagct
     6121 ttacttacct tttgaccgaa ccatttcaaa tttgacaaaa aaccagtata ataaggaagt
     6181 atcgaaaaaa tatccataaa aggaatgtgg aaaatggaca accagctgac aaaagaacta
     6241 aaagaaaact ataacatcgt ttttcacgat ctaaatttgc ttgaacaagc ttttacccat
     6301 tcatcctatg tgaatgagca tcgcaatctg caattatcgg ataatgagcg acttgaattt
     6361 ttaggagatg ccgttttaga actgatggtt tctcagtatt tattccgttt atatcctcat
     6421 ttgccagaag gaaaattgac aaaaacacga gctgcgatcg ttcgcgaaga cagcttgtcg
     6481 aaatttgcca aagaatgtca ctttgatcaa tatgttttgt tgggcaaggg agaagagaat
     6541 tcaggaggac gtacgcgacc agcattgctt tgcgacctat tcgaagcttt tttaggcgcg
     6601 ttatatcttg accagggatt cgacacgacc catgcattca tcgaaaaagt gatttttcct
     6661 aaagtcaaag caggtgcttt ttcacatgag atggatcata aaacaaaact acaagaagtc
     6721 ttgcaaaaat caggtgatgt atcaatcgaa taccgattga tcaatgaaga aggtccagct
     6781 catgaacgag tattttggat cgaagtttat gtagatgatc aattgattgg cactggacaa
     6841 gggaaatcga aaaaattagc agaacaagct gcggcggaaa atgcattggc agcactgtaa
     6901 aagtaagtga gggaaagctt tgtatttgaa acgaattgaa atagccggtt ttaaatcatt
     6961 tgcagacaag acagtcattg attttgaaaa tagcgtcaca gcagtggttg gaccaaatgg
     7021 cagcggaaaa agcaatatta cagaggctat ccgctgggta ttaggagaac aatctgctaa
     7081 aagtctgcga ggcggaaaaa tgccggatat catctttgcc ggttccgata cccgtaagca
     7141 gctgaatatt gcagaagtca cagtgatttt agacaatagt gatcattatc tgccattgga
     7201 gtacaatgag atcagtgtaa cccgccgtta tcgacggact ggggaaagcg agtttttcat
     7261 caataaacaa agctgcagat taaaagatat ccaagaattg ttcttagatt ctggattagg
     7321 aaaagaatcg ttctcaatca tttctcaagg aaaagtagaa gcgattttca gtagtaaacc
     7381 ggaagaccgt cgtgggatct ttgaagaagc agctggtgtc ttgaaataca agcagcggaa
     7441 aaagaaagca gaacagaagc tttttgaaac agaagacaac ctcagccgcg tccaagacat
     7501 catccacgaa ttggaagaac agttaactcc tttggccgcc cagagtgagg cagcaaagga
     7561 gtttttacgg ctaaaagaaa cactgactca aaccgatgtc tctttgatgg ttgctgaaat
     7621 caagacagca aaaaaagatt gggacaacaa acaagcacag cttgcgaaat tcaatctaga
     7681 attaggcaaa ctatctgagt cgatccaaga acaagaatcc attttggcga agcagcgtaa
     7741 agaaaatgcg caagccgatc gtctgatcga aaaaaatcag caagtattat tagatctttc
     7801 ggaaaaactg aagcagacag aaggacaaaa agatgttttg caggagcgga caaagcatac
     7861 acaaaaaagc tcgcaagaat atcaaacttc attagcagaa gcacaaaaaa aggttaaaca
     7921 ttttgaaaag cttcaagaaa gcttgatgaa agcagcagca gaaaaagaaa cagagatcca
     7981 aaaagctgaa gcaaatctca taaaaacaca gcaagaatta gaaaaatacc aaaaatcaac
     8041 gaaagaatta ttggcagaat taagagatca atatgttgat ttgatgcaag aacaagcagc
     8101 tgtaggaaac gaactgaagt atctagaacg tcaatatata caagaaacag caaaaagcaa
     8161 gcagacgtta gccaagcaat cggaagtaga agcatcagta gaccgattga tcttacaaaa
     8221 agaagaattg acacaaaagc aggcgcagct aaaatcatct ttaactgaaa caaaagagaa
     8281 gttagaaatg atccaacaaa acgggaaaaa gttccaagaa gcattagcaa aagaacagcc
     8341 taaaatgtat caattaatga atcaagtcca gcagctacgt gctcgtaaga aaagccttca
     8401 agaaatccaa gaaaactatt ttggttttta ccaaggggtt cgattggtct tgcagcataa
     8461 acagcagctt tcgggtattg tcggagcagt ggctgaactg atcgatgttc ctgcagattt
     8521 cacgttagct atcgaagcag ctttaggtgg ggcagcccag catgtcatcg ttgaaaatga
     8581 aaaagatgct cggcaagcca tcacgtattt gaaacagcaa agaggaggaa gagcaacatt
     8641 ccttccactg acgacaatca aaccaagaca attaccggct catatattga cacaagcagc
     8701 agctgtagaa gggtttatag ggattgccag tgaacaagtc agttatcccg atcaaatcca
     8761 aactgtggtt caaaacctgc taggaacgat ccttttagcc aaagacttaa ctagtgccaa
     8821 tgcaatcgcg caaacgatcc gctatcaata tcgagtggtt tccttagaag gtgatgtgat
     8881 gaatgccgga ggatccatga caggtggagc gaataaacga ggaaaccaag gcagtttgtt
     8941 tgtacaaaat caagaattga aacagttgac tagtgaattt gaagaagcag ataaacaact
     9001 ccaagcacaa gaaaaaaaag ttcaggagct gcaacaagaa acagctcgtt tagccgaaga
     9061 acaagaagtt ttgcgtacac gaggcgaaca attacgtttt gaagaacaag aagcgacaaa
     9121 tcagctacaa aacatcataa atgaacttga acgttttgaa aaagaaaagc aaatctcaac
     9181 ttttgagaca cgtgaactgc agcagtttat cgaagattac gaaaagcaaa cgaacgaatt
     9241 gaaagacaaa caaacagatc ttgaatcgca acgacaaaaa atcgacgaag aaatcaaatc
     9301 gttaagtcaa gaaagcgacc agatggaagc cagaagagca caagtacaaa gtcaaaaagc
     9361 acaagaacaa gcagatcttg ctgtcttgaa agagcagttt aatcatctgc agatccagct
     9421 ccgcggtgcc cgagtccaaa aagcagaggc aaccgaacgt caagaagcga tagaaaagca
     9481 gttggcgaca ttgacagcag acttttctga tcatgaagtc acagaagaaa gtttggaaaa
     9541 acaaattaac gaattatctg ctcagcgaga aacattaaaa gccgagttgg caaaagccaa
     9601 agagcagcga gatagaaggc aaaaggagat cgataagctt gaagcagtct tggcagagcg
     9661 taatcgtgag caaaaagcac gactgtcgga acaatccaaa ttagaagtcc aaaaagaccg
     9721 agctgaaatg attttagata atcatttgac ctatctgcag tcagaatatc agctgacatt
     9781 tgagaaagct tcccaagact atcaagaaac aaccgatatc gaagacagta gaacaaaggt
     9841 ttcatcctta aaagaacaga ttgaaaaact gggaccagta aacttaaatt caatcgaaca
     9901 atacgaacaa gtcagtgaaa gacatacatt cttagcaaca cagcgagatg atctgttagc
     9961 agcgaaaaac caattgtttg aaacgatgga tgagatggat gatgaagtgc ggacaagatt
    10021 caaagaagta tttgaagcta tccgtcaaga attcaaaatt gtctttccaa atatgtttgg
    10081 cggcggtaaa gctgaattag tactgacgga accaactgat ttgctgaaaa caggtattga
    10141 gattgaagta caacctcctg gcaaaaaact gcaaagtcta agtttgcttt ctggaggaga
    10201 acgagcactt acagccatcg cgttgctctt ttccattatc cgtgtctgtc ctgttccgtt
    10261 ttgtatctta gatgaggtag aagcagcgtt agatgaagcc aacgtgaaac gttttggtcg
    10321 ttacttgagc gatttccaag atgacacaca atttatcgta gtcacacacc gcaaaggaac
    10381 gatggtcgca gcagatgtac tgtacggagt gacgatgcag gaatccggtg tatcaaaaat
    10441 cgtttctgta cggatggaag atatcaatga agaggcgaaa atcaacgaaa cgaaagtgta
    10501 ggaacgaaaa tgatcaaatt agtagcaatc gatttggacg gtacattatt aaatgaacaa
    10561 aagctgattt cagatgagaa taaacaagca ttagcacaag caaaacagca aggggtcaag
    10621 atcgttctat gtacaggacg ccccttagct gcgatggcgc attatctgca agaattagga
    10681 ctggtagatg aaggtgacta cagcatcact ttcaacggcg gtttggtaca aaaaaatgat
    10741 actggtgaaa tcatggaaaa gaaagtgatg gaagtatcag atatccaacg cttatacaaa
    10801 ctggctcagc aattggatct gccactagat gttttgtctg acaatgtggt tttacagctg
    10861 cctagtgccc caaggaagca atccttgtac aatgtgttga ataagctgct gcaattccaa
    10921 ccagcggata taagcgatat tacagatgaa tttgtgctta ataaggcagt aatcgcttat
    10981 gaccaagaag aattagatcc taaaatcaaa gaaataccag cagaattcca tgaaatgtat
    11041 gagatcatca aaacaagaag tatgcttttg gaatttatgc caaaaggtgt tacaaaagct
    11101 tatggtattt ctttattggc aaaagatctt ggtttgaaac aagaagaaat catggcgatc
    11161 ggtgatgagg aaaatgattt accgatgatc caatatgctg gactaggcgt agcgatggag
    11221 aatgctgtgc ctttcgtcaa agaagcagca gatttcgtga cagccagcaa tgaagaaaat
    11281 ggcgtagcca aagtgatcca aaaatttgta ttagagtagg gggagtcaaa tgggattttt
    11341 tgataaaatc aagcgtgctt ttactggcga agagaaaaca gaagaagttg aagtacagga
    11401 gaagtacgac aagggtttag aaaagacgcg taaaacattt ggtgaacgga tgaatgagtt
    11461 gtttgccaat tttcgaacag tcgatgaaga cttttttgaa gaattagaag aaacacttat
    11521 cggtgcagat gtaggttttg aaacagcgat acgaatcacc gaagcattac gtcaagaagt
    11581 gaaactcaga aatgcgaaaa aatcggcaga agtccaaaat gcgatcatcg agaaacttgt
    11641 ggatctctat gaagcggaag gaatcaatga agtaaatgaa ctaaatatcc aagaagacgg
    11701 actaacagtt attttgtttg tcggtgttaa tggtgtaggg aaaacaacaa gtattggtaa
    11761 acttgcccat cagttcaaaa acgaaggtaa aaaagtattg atggcagcag cagatacctt
    11821 ccgcgctgga gcaatcgacc agttagttgt ctggggcgaa cgcgctggtg ttgaagttgt
    11881 tcgtggcaat gcaggaggcg atccagcagc tgtcgtattc gatgctttgg aaagagcaaa
    11941 gacagagcag gcagatgttt tactagttga tactgcaggc cgactgcaaa ataaagtgaa
    12001 cttgatgaac gaattagaaa aaataaaacg agtaatccag cgggaattac cagaagcacc
    12061 ccaagaaaca cttcttgtat tagatgcaac cactggtcaa aacgcgatgg ttcaagcaaa
    12121 acaattcaaa gaaacaacag atgtgaccgg tcttgttttg acaaaattag atggtacagc
    12181 aaagggcggt atcgtacttg ctatccgtaa tgaattgcat ttaccagtga aattggtcgg
    12241 tcttggtgaa ggaatcgatg acttggagcc gtttgatcca aatgattttg tcgttggatt
    12301 gttcaaagga ttactaaaag aagaataacc catgtctaaa aagcaggagc tgtgacaaaa
    12361 gttgcagctc cttgtttgaa tgattgtttt taacatagct gtgtttttga tgccttcatt
    12421 tttatactta tttgtaagcg gttctcaaac aatttcgttc tttttcttag actttttgat
    12481 tataattaga ttcatatgaa ttggaggtca cttaatgatg aattttaagc aattattatt
    12541 gcatgtcaat gcgcgccctt ttatcgacca agctcgattt ggtatagaac gggaaggaca
    12601 gcgggttgat cttgcaggaa atctagcaaa aaccgatcat ccagcaatct ttggcgatcg
    12661 atcctatcac ccctatatcc aaacagattt tagcgaaaca caaacagaga tgatcacccc
    12721 tgttaccgat tctattcccg aattatttca gtatctcgct gctgtttatg atgtgactgc
    12781 tcgttctata ccgaaagaag agatgatctg gccattaagt atgccacctg ccttaccgga
    12841 aaaagacgaa gagatcatta ttgcaaaatt aaaaaatttc gaagatgtct tgtatcgacg
    12901 ttatttagca aaagaatacg ggaagcgaaa acagatggta agcggtattc atttcaattt
    12961 tgaatttggc gatgagttgc taagaacatt gttcagccat caggaagaat ttcaagattt
    13021 ttccgaattc aaaacagaac tttatttgaa aacagcgaga aactttatgc gctatcgctg
    13081 gatgatcact tatttattcg gtgcttcacc aatgagtgag aagaactact ttttagatga
    13141 atcacatccg caagaacctg ttcgcagtat tcgcaacagt gcgctgggct atacgaatca
    13201 tccaaatgtg aaagtttcct atgcttctat gaaacagtat ttagcagata tcgagcgaat
    13261 gatcgaagaa gggaaacttt cggaggagaa agaattttat acaccgcttc gtttccgagg
    13321 cggaaagaaa gtcgcagatt tagcaacaac aggtgttcgt tatattgaat tgcggaatat
    13381 cgatttgaat ccttatgcaa gattgggtat caatccagag caagttcggt tcttacaatt
    13441 attcctgatg tacatgttat ggacagaaga aaaagaagac tgtgaccaat gggtagcaga
    13501 aggtacgact cgaaataaca aagtagcact agaacaacca tcagatcaaa cagaatttca
    13561 tcaagaaggt agagaaattc ttgaaggaat gaagcaaatg ctggttgaat tggattggtt
    13621 ggattctctt tatttagtag aggaagcatt gactcaaatg gatcatcctg aacaaacgtt
    13681 agcagcgaaa ctctatcaag aagcacagct atcgagtcag caagaagttg ctgtggcatt
    13741 gggacatcaa tattataaag aaagccatga acgtccctat cagttggccg gttttcgcga
    13801 aatggaactt tctactcaga tatttatgtt cgatgccatc caaaaaggtg tccaagtcaa
    13861 agtattagat gagtcggatc aatttttgcg tctgcaattc caagaccatg tagaatatgt
    13921 gaaaaatgca aacatgacaa gtaaggacag ctatatcgtc ccacttatca tggaaaacaa
    13981 aacagtaacg aaaaaagtct taaaagaagc tgggttccgg gtaccaggag gcgcagaatt
    14041 ttcatccatg gaagaagcgg taaaagctta tccgagattt gccgagcagg catttgtgat
    14101 caaacctaaa tcaaccaatt acgggttagg tatcacgatt ttcaaggaag gagctagtct
    14161 ggaagattat caagcaggtt tagccattgc ttttcgtgaa gacagttcgg ttttagtaga
    14221 agaatttatg ccgggaacgg aatatcgttt ctttgtgatc gacggagaag tccaagccat
    14281 catgttgcga gtccctgcta atgttattgg tgacagtatc cgaacagtga aagaacttgt
    14341 tgaagaaaaa aatagtgacc ctttgcgtgg aacgaatcat cgtgcacctt tagaattgat
    14401 ccaattaggt gaattagaac aattgatgtt gaaagaacaa gggttgacga tcgaatcagt
    14461 tccccaagcc aatcaaattg tttatctaag ggagaactca aatatcagta caggtggtga
    14521 ttcgatcgat atgactgatg aattttctga agcttacaaa aaaatcgccg tttctgcggt
    14581 agaagcacta ggagctaaga ttagtggtat cgatttgatt ataccagata aagaaatcga
    14641 tcctacaact gataaaaaag catatgggat catcgaagca aatttcaatc cagccatgca
    14701 tatgcatgtt tatccttttg caggaaaagg cagaagattg acgatgaatg tattgaaatt
    14761 gttatatcct gaagtatttt aaaagaaaaa gcttttgaca gattgtccta tttgatgtca
    14821 atctgatcaa aagctttttt tgcatgtaaa aatttatgtg ttttttcgtt ttaaaataaa
    14881 cgatcccaga agcttttttt cttttttggt ttttcttttt cagctggcga agagttttct
    14941 tctgggtatt ttttttcgat atcaatcaca ggttcattta ctgcttcttt tgctgttaag
    15001 agcaatccga attcctcagg atttgcagtg acgtaatcgt tgactacagt gaaacggcta
    15061 ttgtttttcg ttgctagctg gatatattgg ttttggacag aaaggggtag agccccattc
    15121 aagagtatgg aagcatctgg atgttttttg agttctttta aaaagttttc tttgtttttt
    15181 ggttggatca tttcagcaac agtcatacta agatagcagc gttccctgaa tgtccctaaa
    15241 tatttatgct gttcttcagg gttcactaaa ggtgtaccat aaagtccctt atccaaatgg
    15301 ctttttacat catcttgtgc cataaaaaat accctccttc ttctctttgt ctttcttacg
    15361 attatagcat attcccaata atacctaaaa catatgaaaa agcggttgct tttgttcaac
    15421 gattcactta aaataagaat gaaaatcatt ctcaattaga aaggtggatt ggatgcgcta
    15481 cggacgacta gtagcattat tagttatttt aagtagtatt tcattgttca tcggagtatc
    15541 gaatgtacaa ttgtcagaac tgctccagtt agatgggcaa agcttacaaa tcttgctagt
    15601 gactcgtttg ccacgaacga tttgtttgat ccttgtggga gcgaccagta gtatttgtgg
    15661 gttgatcatg caacatttga cccaaaataa atttgtttca ccaacgactg cgggcacgat
    15721 ggatagtgcg cgtttaggta ttttagtggc tatgattttc ctacctggtg catccttgat
    15781 tgtccgatcc ttgacagcat tttgctttgc ttttgcagga acattgcttt ttttatccct
    15841 tacgcgattg ttcctccaga aaaatcaatt gatgcttccg ttgattggag tcatgttagg
    15901 gaatatcatt ggttcagttg ctactttttt tgcttatcaa ttccagttgg tacaaaatat
    15961 gacttcatgg cttcaaggga attttgcgac tgtgatacga ggaaattatg agttattgta
    16021 tgctgttgtt ccattgttgc tcttggtttg gctatttgct taccgattta cgatcattgg
    16081 tctgggcgaa tcgtttgcag taaatctggg aatagattac cagaagatgc agtttttagg
    16141 cattggctta gtatcattgg ctagtgcagt catgttgatc atggtaggaa gcattccttt
    16201 tatcggagtg atcattccga atcttgtctc tagaatatat ggagatcaag tgcacaaaac
    16261 aattggcgta actgctttag caggtagttt gttcttagta ttttgcgatc ttgtcgctcg
    16321 cgtgttgatt tttccttatg aaataccagt cagtgtgatc gttggaatca tcggtggggc
    16381 tatcttcctt tatcttctga taaggggacg acgatatgct taaaaaaaag ccatctattt
    16441 ttattggtat gcttgttctt gttttatttg ttacaggaat gtacttgacg atccatacat
    16501 atggaaactg gcattttgca tgtgcattga ggagtaagaa aatttttgca tttatccttg
    16561 tctcgctagc tgtcagcagt gccacaatca gctttcagac aattacgcat aaccagtttt
    16621 tgactccttc agtattggga cttgagtcgt tatatgtcct tgtccaaacg gtccttttct
    16681 tctttatcgg aggagtgacg atgcttcagc agacaggtac agtttttttc ttattgaacg
    16741 ttgggctgat ggtcgggctg agtctattgt tatttttctt tcttttgaaa aaaatgagag
    16801 cgaatctttt tttattactg atgtcaggga tgattttagg tacgcttttt ggtagtgtca
    16861 gtaccttttt acaagtcatc atggatccga atgaatatga tttgcttcaa ggcagattat
    16921 ttgcaagctt tggcaatatc cagagtcagt atcttttgcc tgcttctttg atcattatcg
    16981 gctgctggta cctgttattt cgatatcgac atgtgctaga cgttttccat ctcggaaatg
    17041 aacaagcagt aaacctcggg atagacacag atcgttttga atttttgatt ttatgttgtg
    17101 tcagtctttt gacagggaca tcaacggctc ttgtgggtcc agttactttt ttagggttta
    17161 ttgtcgcaaa tatcagttat cgtgtctttt ctacttatcg tcatggtcag ctatttatcg
    17221 gaagctttct ggtagctatg ctgtttctga caggaggaca gctcgcagta gagcagattt
    17281 tcggctggaa tacaacagtg agtgtcgtcg ttgaatttgt cggaggaatt tattttgtca
    17341 caaaattgat tcgcgaaagg aagagtatcc tatgattgat ttacaaaagg tcaataaact
    17401 gtaccatgga aaatcagtgg ttaaagatgt acagttaacg attcctgaaa agaagttgac
    17461 tgcttttatc ggtccaaatg gtgctggaaa gtcaacggtg ttgtcgatga tcagtcgttt
    17521 gataccaaaa gatacaggtg aaatctattt agaccataac gaagtaaagg cttggaaatc
    17581 caatgaatta gccaaaaaat tatcgatcct acgacaatcg aatgaaataa atgtccaaat
    17641 caccgtgaaa gaattagtag aatttggacg atttccttat acgaaaggaa aattgacgac
    17701 aaaagacgag gaaatcgttc aggaatcact ggaacacatt gggttagaag aaatagcgga
    17761 tcaatcgatt gacactcttt caggcggtca actccaacgt gcgtatattg caatggtgct
    17821 agcgcaagac acggattata ttttactgga tgaacctttg aacaatctag atatgaattt
    17881 tgcggttcaa atcatgcaga ttttaaaaaa tttagtacaa gaaatcggga aaacaattgt
    17941 tattgttctt catgatatca actttgctgc aagttatgca gacgaaattg ttgcaatgaa
    18001 agatggacag ttatttacgc aaggaacgac ccaagaaatg ataaaaaaag atgtattgga
    18061 tgaattatac gaaatggaca tacggatttg tgagattgaa ggccgcagat tctgtcttta
    18121 tttctaaaac taacattagg agagaagaga tgaaaaaatt agtagtaggt atcttgattc
    18181 tagcatcatt tggtttagca gcatgtggaa atagcaacaa caccagtcag gcagacacaa
    18241 aaaaaagcag cacacaaact gaaactacat tgacgataac agatagtaat ggagaccaaa
    18301 tcgaggtgcc aaataatcca gaaaaagtcg tcgtattcga caatggttct ttagatacga
    18361 tggatgctct tggtgttgga gataaggtag ttggagcagc tacaagcagc cttcctgagt
    18421 atctttcatc ctataaaaaa gtagaatcag caggtggaat aaaagaacca gacttagaaa
    18481 aaattaatca gcttcagcca gatctaatca tcatttctgg ccgacaaaga gattttcaaa
    18541 aagatctaag tcaaattgct ccgaccattt tcctttcttt agatgcgaaa aatccatggg
    18601 aatctttcca gcaaaatgtc actgctttag gggagatctt tggtaagcaa gaagaagcta
    18661 aaactcaatt agaggaatta tctagtgcaa tcgatcagac gaaaaagaaa gcagaagcta
    18721 ctgacaaaaa agcactagtc acacttgtga atgaagggca attatcagcc tacggatctg
    18781 gttctagatt cggtttcatc catgatttat ttggctttga acaagcagat gatcaaatcg
    18841 aagcatccac tcacggtcaa agtgtttctt atgaatatgt gctggaaaaa aatccggata
    18901 tcttatttgt agtagacaga acaaaagcta ttggtggaga cgacagcaaa gatgatattt
    18961 ctgccaatga actggtagca caaacgaatg ctggtaaaaa tcagcagatc atctcccttg
    19021 aaccagatgt ctggtattta agcgggggcg gtttagaatc aatgaaactg atgatcgaag
    19081 atgtgaatca agcatttaaa tagcataaat aacagaggat taaactctca tactatgtaa
    19141 caataatgtt tcttacattc ttaaatagca agagcctatc ctcaaagaag ttatcaaaaa
    19201 ccatccgtaa aatcgtattg atgattttac ggatggtttt tatttatttg agaaatcttt
    19261 tttcaacgtg aattcattat ctacatcata cgggttgatt ctagaatggg gagtggcaaa
    19321 aaatagtgtg atccattttt tcagcatcaa gatcgcctcc tttgatttga atcaaaggta
    19381 caaagcgaaa cttagataaa acttaaattt agatattttt attttgtacc gatctcttct
    19441 ttaaacgatg ttccatctgc tgcttctcgg caataagtcc actagactag aaaatatgag
    19501 aagctatttt ctgtccagcg ttcttattac tcaaaacaaa tcgcagttgt cacaatctct
    19561 tctaaacgat gttccatctg ctgcttctcg gcaataagcc cactagacta gaaaatatga
    19621 gaagctattt tctgtccagc gttcttatta ctcaaagcaa atcgcagttg tcacaatctc
    19681 ttctaaacaa tgttcaatca gctgaccttc ggtattagct caaagcactg atttcacaac
    19741 ctctatttag gcatgtgtct cagcaagcgg ttgatagttg atgatctgca tttttcgata
    19801 aaaatcggcg ggaagcttac tccaccgttt tagtaattga tagaaataac tctcttctgc
    19861 tttgatttta tactcgctta atatcaatcc aaaactattc gttttttttg aagctaattt
    19921 tttataaata tccaatgctt tggttttatc ttcattgaca aagagtgttt ttacttcaat
    19981 ataagaaccc tcgatatttt gtgtttcttc gataaaatag atgtttttta tatattcctt
    20041 catagtgatt cctccaaaga agtcttttat ttgataagtt tactctaccg aagagttgtt
    20101 aataatccgt gaacaaaaag taattctttt tccaatagtt gttaaaggtt ttttattttt
    20161 tatagtattt tttgttattt aagaaaggaa taaggttaaa caaataacag gttgcttttt
    20221 gggtcttttt ccgttcaaga aactgaacga agaaaaggga aagttgtctc ttttgtgata
    20281 agagaaatca taataaacga atgacttact ttccaataaa ttgagaacag tcagaaccag
    20341 acaaattagt tgcatctgat gaataaagtg attattctat gacatgaggt gatacagatg
    20401 aaattagaag tgacaaaaga agcacaagaa gtattgaaga ataaattaga agcagacgat
    20461 caacttttat tagatatcga aaacggtgac ggtccatttg cagaaagcca agtcagttgc
    20521 caattggata cggcatttcg attgatcatt gtcaaaaaag atacacaaac agatctaagt
    20581 ctttattctg tagtggctga tacaccagtt ggaccaatca aaatcaaaga cagtgcaata
    20641 ctttacatgg atgatccatc cacacttgct ttagaaccaa cttataatag tttacaatta
    20701 aaaggaccaa gcggcttgag aaaaggcaac ttgcaagttg ttcgaaaaga ctagagggat
    20761 aaaatgggaa aattggcgat catcagcgac ctacatgtgg acatcaataa attcggcgaa
    20821 tcagagctga tgcagctttg gcaagttttg caaaagcaca agatcactcg gcttcatcta
    20881 gccggagata cggctaataa agtagaccgt tgcctagctg ttgtcgaatt tttcgtcaat
    20941 aaaggattat ctacgacatt tcatttcggc aatcacgagt tggcagatgt gcccaatgaa
    21001 cagatgatgg accattttcc agacccacat tttttaaatg aacggtatat tgctttaaat
    21061 gagcagaccg ttcttttagg gatgaatggc tggtacgact atcagttttc cgaattgaaa
    21121 gattacgagg caatcgtacg aatgaagcgt ttgtattggt atgatcggtt catccagcgt
    21181 gggaaaacag atctcgaggt caatgcagaa atgcttgcag ctgcaaaaaa tttgctagac
    21241 cagctggaag caaaaaaact tgaggtgatt ttatccactc atttcgttcc taaacgggca
    21301 tttatcatgt atcaatctgc tccttatgag cgttggaaca aattaaatgc atttttagga
    21361 tcagaatcat tcggagaatt acttgaccgt tattcaaata tcaaacaagt agtttttggc
    21421 catacgcatc gtcgctttga agataaatcc atcggtcaca cgatttacag ctgcagacct
    21481 tttggttatt attatgaatg gcagctgacc cgagattttg tcttgaaaga acagttgcta
    21541 gacagctatg atcctatgaa attacgtgtc ctcttgcgtg cccatcaccc agcttttcag
    21601 gcctatcaaa cacaacattt gcaagaggaa tttgaacaag cgatgacaat cgttccgtat
    21661 tgagacagca ttagtaatgt gtaaaagaaa ataccttgta aaacataagg ataagtaaga
    21721 gcagtagatc catttatcgt ggatatgctg ctttttttgc ggaaaataat cagcgaatgt
    21781 ttatttgatc ggatgatttg taactcgcag agtactagat gcaagcgtgt attctttttt
    21841 tgaacgataa tcacaaaaaa aaatataata tacatttttt atttaattat aattaatatt
    21901 gattgaagtc taggcagtca agtcgagagt gtaaaatatt ttgtgtaaat gaaaaaatcc
    21961 atacaaaaaa ggaagtc
//
LOCUS       DAYYBG010000045        21973 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000045 DAYYBG010000000
VERSION     DAYYBG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.045"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1432)
                     /locus_tag="ACHAUN_001595"
     CDS             complement(41..1432)
                     /locus_tag="ACHAUN_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8460299.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1689..5078
                     /locus_tag="ACHAUN_001596"
     CDS             1689..5078
                     /locus_tag="ACHAUN_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8460300.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            5081..6502
                     /locus_tag="ACHAUN_001597"
     CDS             5081..6502
                     /locus_tag="ACHAUN_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8460301.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6499..8376
                     /locus_tag="ACHAUN_001598"
     CDS             6499..8376
                     /locus_tag="ACHAUN_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8460302.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8468..9310
                     /locus_tag="ACHAUN_001599"
     CDS             8468..9310
                     /locus_tag="ACHAUN_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8460303.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9462..10337)
                     /locus_tag="ACHAUN_001600"
     CDS             complement(9462..10337)
                     /locus_tag="ACHAUN_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8460304.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10544..11574
                     /locus_tag="ACHAUN_001601"
                     /pseudo
     CDS             10544..11574
                     /locus_tag="ACHAUN_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11663..11968
                     /locus_tag="ACHAUN_001602"
     CDS             11663..11968
                     /locus_tag="ACHAUN_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8460305.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            12147..14162
                     /locus_tag="ACHAUN_001603"
     CDS             12147..14162
                     /locus_tag="ACHAUN_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8460306.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            14164..14448
                     /locus_tag="ACHAUN_001604"
     CDS             14164..14448
                     /locus_tag="ACHAUN_001604"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8460307.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14474..15847
                     /locus_tag="ACHAUN_001605"
     CDS             14474..15847
                     /locus_tag="ACHAUN_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8460308.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15930..17945)
                     /locus_tag="ACHAUN_001606"
     CDS             complement(15930..17945)
                     /locus_tag="ACHAUN_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8460309.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17967..19181)
                     /locus_tag="ACHAUN_001607"
     CDS             complement(17967..19181)
                     /locus_tag="ACHAUN_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8460310.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19401..20345
                     /locus_tag="ACHAUN_001608"
     CDS             19401..20345
                     /locus_tag="ACHAUN_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8460311.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20641..21585
                     /locus_tag="ACHAUN_001609"
     CDS             20641..21585
                     /locus_tag="ACHAUN_001609"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8460312.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7478 a         4007 c         4137 g         6351 t
ORIGIN      
        1 ttttatcagg gtggtttata catagctgtt ttttgattat tcataactcg aataagcgga
       61 tgagttcttc ttcatttgcg aatgacacac tatcatcaat caatatatga ggaacatggg
      121 cattcaaaat gggttgattc gtaacgatca gatcatactc gagaagattg tatttcgaca
      181 gctgatcttc agtaatagca gttgtctcag agtgaatttt atgcacagca agaatatcac
      241 tgatatattc ttcaatatat tgactatgtc gaagacttaa gtcgctgata atcagtattt
      301 ttttatcaga agtataatgg tgaatttcag gattaccgat aagcatccaa aagatatagc
      361 tagataaata ataaatcaga ttctgattca gtagagtgga ataaaattga agttctgcac
      421 gaaatccagc atatagacgt atctggcggt gttgcatctg ctgtgcaaag taagttgcac
      481 ggatcgtcaa atgagtcgta tcgtatggga aaatacggga gaaaaaataa atttgcgtta
      541 ataattgaac aacatattct tcgttttttt ctttgcaatc agcggatgaa aacgaacagt
      601 aggacatatt ttttcgtatc tgctcactga tttcattttt ttctttctcc gaaaattctg
      661 aaaaaatcgt attgacaaaa tagttcacta cttttatata gctgctgtac ttcaattttc
      721 ctaagagatt tttcaagatt aagaagtctt ttttatcgat atttatcaac tgtacataag
      781 agtctccagc tttccattca aatggagttc cttgggattc tctcatgaga tgcacaacaa
      841 aatacataat aaagaaagat tgctcatatg ggctttcttc ctgaaacaaa ttcatctgac
      901 aattattctt gaccataatt tctttgatct tctttttttc gattggaaat ggcaacgtgc
      961 gtcttccgta gtaacttaaa aagaaaaagg taacaaagat acgcaactgg agctcacttt
     1021 taaggctagt taactgaaaa ccttggtcaa cgatcacttt taataaatag ctttctaaaa
     1081 tttgattgca ttgtttcaac ttacgtgtaa aagaagaccg gctgatggaa attttttcac
     1141 aataatactc aatcgtttta cccggattga caataatctc atttaataaa cgactgtttg
     1201 tagaactttg cgacatttcc atcagaacat ttaaataagc tggtacattc aaatgttcaa
     1261 gacgataccc caatttttgt gaagattgaa tcgttacatc cgttccccag cggattttga
     1321 tctcttcgat ataccttaca aatgtagatt ctgaactacc aattcgtttg gcaagttctt
     1381 ttttagtcat ccacttgtca gaaatggaca gaattcgaat gattttttcc atctttctat
     1441 agtttaattc caacaacctg atcatatgtt tttctccctc aacccttttc tttcaatgaa
     1501 acgctcatta atcgaaggaa tcaacttcat aaatgaaacc aattggtatg aaaattggtg
     1561 tgattccctt tagcgccctt cgtataataa ttatcatatt aaaataaata aaattatgta
     1621 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
     1681 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
     1741 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
     1801 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
     1861 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
     1921 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
     1981 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
     2041 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
     2101 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
     2161 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
     2221 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
     2281 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
     2341 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
     2401 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
     2461 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
     2521 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
     2581 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     2641 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     2701 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     2761 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     2821 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     2881 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     2941 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     3001 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     3061 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     3121 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     3181 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     3241 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     3301 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     3361 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     3421 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     3481 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     3541 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     3601 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     3661 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     3721 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     3781 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     3841 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     3901 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     3961 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     4021 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     4081 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     4141 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     4201 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     4261 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     4321 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     4381 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     4441 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     4501 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     4561 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     4621 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     4681 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     4741 gacaagaaac agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     4801 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     4861 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     4921 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     4981 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     5041 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     5101 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     5161 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     5221 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     5281 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     5341 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     5401 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     5461 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     5521 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     5581 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     5641 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     5701 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     5761 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     5821 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     5881 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     5941 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     6001 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     6061 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     6121 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     6181 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     6241 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     6301 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     6361 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     6421 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     6481 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     6541 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     6601 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     6661 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     6721 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     6781 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     6841 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     6901 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     6961 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     7021 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     7081 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     7141 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     7201 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     7261 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     7321 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     7381 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     7441 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     7501 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     7561 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     7621 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     7681 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     7741 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     7801 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     7861 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     7921 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     7981 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     8041 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     8101 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     8161 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     8221 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     8281 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     8341 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     8401 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     8461 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     8521 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     8581 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     8641 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     8701 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     8761 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     8821 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     8881 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     8941 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     9001 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     9061 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     9121 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     9181 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     9241 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     9301 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     9361 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     9421 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     9481 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     9541 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     9601 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     9661 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     9721 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     9781 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     9841 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     9901 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     9961 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
    10021 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
    10081 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
    10141 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
    10201 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
    10261 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
    10321 ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa cacttggttc
    10381 cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca aaccgaatga
    10441 taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact ttctgcgctt
    10501 ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta caatcgctta
    10561 tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa aacttacgaa
    10621 caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa cattcgaaga
    10681 catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc aattagttgt
    10741 catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg aacatggtaa
    10801 gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag agttatttgc
    10861 attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt tcgattctga
    10921 cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt tagaagtaga
    10981 aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat tttctactgc
    11041 gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt cttatttcgg
    11101 cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac gaatgaatga
    11161 ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat ccagttattt
    11221 ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aagggatgtt cgtgaaacaa
    11281 acaaaagatc gacaagaaga acatctgaaa atgttttaca tgcctgatca tgaagacttt
    11341 ggtatcgatt tgcccgaaca ttatggtgta ttgacctata tagatgaaca agggagttat
    11401 catgaagaaa aagtaatttc cgaggttggt gattacagcc gtgtctacga agggatttac
    11461 gagacattag ttaatggcgc agaaaaagtc atcaaagatg aagaaacaat cttacaaatg
    11521 aaaattttgg aagaaggaat caaaagcatt caagaatcag aaggaaaaat atgacatatt
    11581 tgtaagaatc gggtttgttt tttgacgcat aaactgtctt attgtacgat gaaaaagtat
    11641 agaactacta ggaggaacca acatgacaaa ttatgacggt gaaaaacaca aacgattaag
    11701 aggttcaatg gcccctaaaa cagaagaaga agtggcttta gcaggaacta attctggtga
    11761 aacagaagac aaagaagaat cattagctgg cacaaactct ggtgaaacag aaaataaaga
    11821 aacagcttta tctggttcaa attctgtaga agaggaaaca aagcctaatg agcccaatca
    11881 ctttgaaaaa gatatcgaaa aagcggttga agatgtcaaa gaaaaatttg aagaactgac
    11941 ctcaaaaaat aaacatgatc atcattgatc ggacgaaatc aattcataaa agcaggagac
    12001 atttggtcac ctgctttttt tctgtatcca ttcgatagaa atcgttcggt acagtcgtaa
    12061 aaaaaatcca actgtaagtt gtaccaaaca cccctcgatt taaaagcgct accatactag
    12121 aatataagta ggaggtgaac agcgctgtga actacaaaat tagttatctc ctgaaaacac
    12181 caagagaaat cagtctagaa aaagcagcag agatcatcga agtaaataca agcgaggcga
    12241 tcaaacaatt aactcctttt gccgaatatg tggatgatac ccgtatccgt ataaagaaat
    12301 tccgagcaat cgactgggca gaaaaatttt tggatattac tcgatttgaa gctgtgtata
    12361 ccgaacaaga gcgaagagat cttctttatt tgatggttta ttcgactgca acgtctgttt
    12421 ctgtttttca ttttcaagat tttcttcagg tttctaaagg gacagtcctg tcagatatca
    12481 aaaagataag aaatgaatta gaagtggaag attgtcagct gtcgtatact aggaaaactg
    12541 gttttaggct gctagggtca gaagcaatat taagaagaga agcaaaaaat cgcactgctc
    12601 gtttgatgca gtcacttgct ggaagatttg ggctcacgta tttaaccgca accctttcca
    12661 ttgattatta cgccaaagtt agagatttgc tagataaaac gatttcacta tcagatcttc
    12721 gattcgcacc aaatcgaatg gatgaattag cttattattt gagtttgaca accattcgaa
    12781 ttggaaaaac atccatggaa ctggtaaaag atgctgaact gttggcttct cttgcagtca
    12841 ctgacattag tcgacaagta ttgactggcc ttggctattc cgaacatcaa aattcggaga
    12901 ttatattttt ctctctttgc ttagcatcga tcgttgaagg agatgtccag gagcctgcac
    12961 ttgatttttt gctggaatgt tcttcagaag tcatccatcg catggaattc ctgatggccg
    13021 tagaatttgc ttcgtttcgt gaattattga tgaatgtctt ttatcacttg gtccctgcct
    13081 attttcgtat ttcctattct ttttatttac ctaatgtgat gatcgatcaa attaagcatc
    13141 aatatgcttc catctacgaa atgacaagaa aagcattact tccgttagaa agacgaatag
    13201 ggaagagcat ccctgaagaa gagattggtt ttttcactat actatttggc ggagagatca
    13261 gaaaagcgga cgcagaagaa agaaatagaa aaataagagc cgtgatcgtc tgtccaagtg
    13321 gcattagctc ttcattgatc ttaaaaagtg aacttcagca attattcccg atgatctttt
    13381 ttacagagac taattcagct gaccgcttac acgaggttga cgaaacttct tacgatatta
    13441 ttttttctac tgtacctctc acacttactg ataagaaaaa gtctctctat gttctgcatc
    13501 caatcatgac tgacttggaa aaaaatcagc tgatcaatca agtacaaaga gattggctga
    13561 taccaggatt ttcaatgcct tctgcccaag aattgttgtc ggcgctactg ccttatatcg
    13621 aattaaaaga aggcgtagac gaagaaaagc tctatcaagt attaaatcgt aaaatgaaca
    13681 aacttttaga aaagaaggag gatcaacgtc ctatgctatc tgaattatta acagaagaaa
    13741 tgatccaatt atcggatcaa ccaaaaaact ggcaagaagc aatcacattt gccgcacagc
    13801 cgctattatc agaaaataaa atagaagaaa gttatatcaa agctatgatc gagcgcgtgg
    13861 agcagtatgg tgcgtttatc catattggcg atcacatcgc attacctcat gctcgtccag
    13921 aagatggcgt aaacgaagta gggatgtctt tgctgaaact tcaacaacct gtggatctgc
    13981 ttgataatcc agcccatccc attagtttgt tcatctgttt agctgctatc gataatgagg
    14041 cacatcttcg ggctttggca aatttaacaa agctactttc taataaacaa aatcttcaag
    14101 atttattaca cgcatcaaca aaagaagaaa tattagcgat cataagaaaa ggagacgaat
    14161 gatatgaaaa tttttgcagt atgtcaaagt ggattaggaa caagttttat ggtagagatg
    14221 aacatcaaac aagcacttgc agatcttgga gcagatgtgg acgagtttga agtaagtcat
    14281 acggatgttg gaagtgtatc tagcgatatg gctgattatt tcttcattga gcattctttg
    14341 aaagatacgc taacttctat acctgatgaa aagctcgtac ctttgcaatc gatcattgac
    14401 agcgaagaag taaaagaaaa agtaacgaag atcttgtcaa aagaataaaa atgattcaag
    14461 ggaggaacta tcaatggata aattgttgaa tttgtttgtg tcaatcgtca gtcagccctc
    14521 catcctcgtt tctttgattg cactgcttgg tctcgttctt cagaagaaga aattttcaga
    14581 agtgatccaa ggaactatca aaacatttgt cggattccta gtactgatcg gcggtgctgg
    14641 tttaatcagt aattcactaa ccccttttgc ttcgatgttc caaacagctc tgcatactca
    14701 aggagtagtt cctagcaatg aagcagtagt ggcgattgct ttgcaacaat acggtacacc
    14761 tactgctttg atcatgctag tagggatgat cgtgaatatt ttacttgcta gattcacaag
    14821 atataaattc atttttctta caggtcaagc aatgctttat gtttcttgtc taacagcagt
    14881 cattttagta agcgcggggt ttactgcaaa tttgtggatg atcttattag gcggggtctt
    14941 cgaaggaaca ctattaacta ttacgcctgc tctttgtcaa ccgtttatga agaacatcac
    15001 tggaaacaat caagttgcgt tagggcatac cggaaatatc ggttatgctt ctagtggatg
    15061 gatcggaaag ttttttggaa acaaagaccg ttctacagag gacataaaga ttcctaaaag
    15121 tttcagcttt ttgcgagatt ctactgtatc gatcatgctc ttgatgtcga tcgtatatgt
    15181 cgcacttgct ttgttagctg gtcctggatt tgttgaaaga gaactaagca atggagacaa
    15241 cgctgtaatc tttgcactga tccaagctgg aacattcaca gctgggtttg ttatcgtcct
    15301 tcaaggggtt cgaatggtgt tggcagaaat cgttccagca ttccaaggga ttgctaaaaa
    15361 actcgtaccc gattctaaac cagcattaga tgttcctatc gtttttccat atgcacctaa
    15421 tgctgtatta attggctttt ttgttagctt ctttgtagga acactatcca tgtttgctat
    15481 gataggcttg caaactacag ttatcattcc tggggtagta ggacatttct tctgcggagc
    15541 tgcttctggt gtcttcggca actcgactgg cggtagaaga ggggcggtat taggttctgc
    15601 tttcaatagc ttattgatta gttggctgcc attatttatc cttccagtat taggagatct
    15661 taaaatcgct tcttctacat ttgccgatac tgattatcta gtaccaggac ttgtacttgg
    15721 gaaactagga gaatacggtc aaatagcctt gacagttgga atcatcgctt tctttttact
    15781 tgtcgtatta tctagctttc tttttgccgg aaaaagaaca aacattgttt ctaaagaaca
    15841 gaattaagca aacttcaaaa taaaaattgc ttggacaata tcagaagaag atttaaagtc
    15901 ttctgatatg tcgcaagcag ttttttgttt tatgcattta tttttgaata gcttgttaag
    15961 acaagtggct gttttgctag aagaggataa cttttaccat tcattttcac tgttccgcct
    16021 gttctatcag aagtaatcac gatcttatct tttgtttttt caagcgaaat ctttccatct
    16081 ttgataggta gcgtacaatt cagtgtttca aatgaatcta gattaggttg tatcataaat
    16141 tcttcataac ctggctttgt tggatacaaa ccgataaaat agcgtcctaa aagatagatt
    16201 gggcttgctc cccatgcatg gcataagctt tttccataag gatctccata catactgtac
    16261 ttttcatttc ctgtttgagt agggtcgtat tcttcccaga aagttaccgc atcatgagat
    16321 aacatccctc cccaataatc aagaatcgtt tggtagacga tattcgtctg tccgagtttg
    16381 cataaagcat cttgttcata gaatttgaaa tatggagtag taatctgtgt gatagtgtca
    16441 ttcaataaga cattctttaa gatcgattgc tgttggtctt tgttagcaaa atcgaataaa
    16501 acagcgaaaa tattcgcatg acgcgtcaca tttcttctcc cagattcata gcaatcgata
    16561 aaagcgccaa gttcttcatc ccaaaagtag tcaaataggt tttgtttcaa tagttgatat
    16621 ttttcttgat agagatgagt atgataattt tcgttctctc catatgtttc agctaagata
    16681 tctccgcaag tgatcatgga atgataggct ttcagtaata agacttgttc tgcagcaagc
    16741 gttccttctt tatccatttc agaccaatca ataaatatcc aatcttttgc tctcccataa
    16801 ataaacccta attcattcgt ttgctccatc aagtatctca ttaatgattg catcttaggg
    16861 tagacgatgc gtaaaaattc atagtctttc gtcatcatat agtggttttc gataccaatc
    16921 aaccacaaga tcgaatagtc aacgatagta ttcatatgct gcttgatttc attttgtcct
    16981 cttaatgcta atatcgttcg tttattgatt tcctcgtcaa aaaaaagata ttgattgatc
    17041 atgaatgatt gatacgcatc tccagaccaa atccagcgat ctcgtttgat accgtcgata
    17101 aaaaacaaat cactacataa cttgaaggct tcttctgata ctgaccagat ctgattcagt
    17161 aaagaagaat cgcttgaaaa agcagcttgt ttcgaaagag ataaaaattc atgaaaaact
    17221 tgaagttgaa ccattccagt agaaacctct ggtataaaca aatagcgaaa agcgcgttta
    17281 cgagctttca tttttcttgt cattcctatt tgcttatagt agcaataatc gatatccata
    17341 gcttctacat cggactctcc ataacataga gtgatagatt cgatatggtc atccagagat
    17401 attagttcta cacagccatt cactgctcgc ccaaaatcga ataagattcc tccgccaatc
    17461 tccgtttcag tgactggatg gacgcattct ttttgataat agatttgatt tggatctttt
    17521 gcgatatcga cataaagcgg actccagcca gctggatatt cttcaataaa attacttgct
    17581 gtccagccaa tatctgattt tacttcgtct ccctcgataa acatcgctgg tagccctgat
    17641 gtatgtccta caaagatacg aatttttgct ttaccagctg gacaagtcag ccattttcca
    17701 catggatgtt tttgtccatt aatttctaca tacccaactc cttggatcgt cactttaaac
    17761 atggaaggtt gatcaagaaa gtaatatctc ttaaacttga cgttccgatg gcaatcatcc
    17821 atgtaccagt acgctggcca gtcaaaacct cgttcttccc gttggaaatt ttgtagcagt
    17881 ccatggcgga tctcaaagtc tccaggatac caaagccatc tatctttatg tttttcattt
    17941 gtcattttac acgacctcca tcaaatttat tttgttgtca attgttcttt tatttcttca
    18001 tctactctta tcactgtctt tctattcgtc ctgtgtacta ctagaaggct aataagcgcg
    18061 ctaatcaaca gcaagatact gccattgacg aaaacagcaa gtgttttact cccaagagaa
    18121 gcaaaagaga gaaagagcaa accatatggt gcaatgaaag tacctaaatt agcgccaacc
    18181 aataagattg aagtattgaa actctccgcg tacttgggcg cttggatact tacttggttg
    18241 aataagtaag gaatgaataa cgaaaaagaa attcctgtta ggatcgcacc aaacccaatc
    18301 acatacaggt ttatcacagt ggcaatcaat acaaagccga ttcccatcag tagtaaagaa
    18361 agtgtaagca tatagcgatg tgcttttgtc tgaagccagc caaaacaaac tccggcagcc
    18421 atacttgcca attgcatgat tgttaagata ttcgctgcat tagtttcgtt tcctagagaa
    18481 taagtcgtca ggactgtggc taatttaatc gatacagcat tatataccac gatgaccagc
    18541 aagagaaaaa taccataact aatcatttta ggattcgttt tgattacttg tcttgttggg
    18601 gctgtgccat gcgattgaag ttcagttgtt tcaggtgtag gaacaaacaa aacaaacaag
    18661 acaagaatag gtaagctgat ggcgtagact aaaaaggtat attgccaacc gaactgtact
    18721 agctgtcctg caacaaatgt cattaaagct gcaccaaatc cttgaaatgc actttgaaaa
    18781 ccaatcatag aggctcgttc atctccgtga taaaaggcag caatcatgct gactgccaaa
    18841 gaattgatca atccaaagcc aatacctagt ccagctcttg aagctaagac aatactgtaa
    18901 ctttgtgaga agagggggag gataccacaa atcgatgcaa tcactaatcc gatgatcaca
    18961 gtttgttttt gtccgatttt cctagcaata aaactactaa tcaaaacaaa aatagtgacc
    19021 ataaaagagg gagcagtcgt taataattca acagcagata aagaacgcgt ggcataagta
    19081 gctgacatac tgggaattgc agacgtgact gcaccagcag aagtcaaaat aagagagact
    19141 gacagcagag aaaatttcat catcttttta ttcataagca tgttcctttc atttttgtaa
    19201 gcgcttatca aaaatatagt atgaagtggt tattctctca atagcggaat cacaggaagt
    19261 tcatgtccca accatagaaa gtctttatga aaccaaaaat agctttgcat ataatagcag
    19321 tacagcgtga actaataagc gaaagaaacc gcttacaaaa aggagggagt caattggaac
    19381 tagctgaatt agacattttt ttgcgtaacg aagaatcaat cgaaactctg cagcgtgaac
    19441 aaggaatgaa tatcaatgat ttaaacttag cttaccatct tcagacaccg ccaaaaatcc
    19501 accgagggga tctatttcaa cagggaaata ttcatatcag taaacaccgt cgatatgcgg
    19561 aagttcccga gcatacacat gattttgttg aaatcaatta tatgtatagc ggtacatgtt
    19621 tacaaattat taatggagaa aggatagaga tggaagaaag tgacgtggtg atcattgata
    19681 aagaagctac tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat
    19741 taaaagaaga aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac
    19801 tttcttcttt tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt
    19861 ttcgcggcgg aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt
    19921 ttgatacata cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg
    19981 aattgactca tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg
    20041 agatgcttag agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa
    20101 cagcagctca ttttggctac aatccggtct atcttagtaa taaattgaaa atgtattttg
    20161 atgcttcctt tcaagaactg gttctaaaga aaaaattcga ctttgcgttg gaactgattc
    20221 aagaaacgag tttttcgttg gaagaaatcg ctaatcgtat cggatataaa caaacgactt
    20281 ctttattcaa attattcaag aagtattcaa catgtactcc caatgaatgg agaaataaac
    20341 attaagaaga aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct
    20401 gtcttattat atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt
    20461 tgtattatca ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga
    20521 attcgtttcc atcaaaaatt ataataacaa ctattataat taacatgttg ataaaatgaa
    20581 taaacaagac tatacttttc atgaaagtaa caaatagtta tgaaaaggag ctgtttattc
    20641 atgaaaaaaa caagtcgtaa agtagtaatc gtcggtacag gttttgttgg aacaagtatc
    20701 gcttatgcaa tgatcaacca aggaatctct aatgaattag tattgatcga tgtgaatcaa
    20761 gaaaaagcag aaggggaagc attggacctt ttagatggta tggcttgggg cgatgaaaat
    20821 gtggctgtat ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt tgttatcaca
    20881 gctggtatca accaaaaacc tggtcaatct cgtttagatt tagttaaaac aaatgcgtca
    20941 atcatgcgcc aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat tatcgttgtt
    21001 gcttcaaacc cagtagatat tttgacatat atcgcttgga acgaatctgg tctgccaaca
    21061 tcacgtgtta tcggtactgg aacaacattg gatacaactc gtttccgtaa agaaatcgca
    21121 ttgaaattaa aagttgaccc acgcagcgtc cacggctata tcttaggtga acatggtgat
    21181 tcagaagttg ctgcatggtc acatacaact gtcggtggta aaccagtgtt tgaaattgta
    21241 gaaaaagacc atcgtattgc ccaagatgaa ttagatgtga ttgctgataa agtccgtaat
    21301 gctgcttatg agatcattga tcgtaaaaaa gcaacttact atggtattgg tatgagtact
    21361 gcacgtatcg taaaagctat cttgaataac gaacaagctg tattgcctgt atctgcttat
    21421 ttaactggtg aatacgacga aaaagatatc tttacaggtg tgccatcaat cgttgacgaa
    21481 aatggtgttc gtgaagttgt tgaattatca atcaacgaag aagaaaaagc aatgttcagc
    21541 aaatcaacaa gtgctttaag agaagtattg aacactgtat tataagaaac aatgaatatg
    21601 aactaaaaat cctaaaactg cttaacagct gaaatagctg ttaagcagtt ttttattgat
    21661 atctaatagg taagcaaata aatagaatgg ttaaaattaa tcggtccgaa aatgtatccg
    21721 tacatatgaa catatgaaat ttaatctgtt gattacaata agcgaatcgt tatcaatgtt
    21781 tatctgtggt taattaaaaa atccttccgc aacggcaaat acaatgattc atatctaatt
    21841 cgttcatatt tgattaactg aataaagaaa ctggttttta tcattcaatt aaacgacaaa
    21901 aaaagtaaga aaaactgcaa aattttttta taattttgca taagcagatt ttgatggaaa
    21961 aatgacgatt ttt
//
LOCUS       DAYYBG010000046        20362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000046 DAYYBG010000000
VERSION     DAYYBG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.047"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACHAUN_001610"
     CDS             291..1430
                     /locus_tag="ACHAUN_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8460313.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACHAUN_001611"
     CDS             complement(1497..2072)
                     /locus_tag="ACHAUN_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8460314.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACHAUN_001612"
     CDS             complement(2077..3051)
                     /locus_tag="ACHAUN_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM8460315.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACHAUN_001613"
     CDS             3334..4302
                     /locus_tag="ACHAUN_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8460316.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACHAUN_001614"
     CDS             4415..4693
                     /locus_tag="ACHAUN_001614"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8460317.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACHAUN_001615"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACHAUN_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8460318.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACHAUN_001616"
     CDS             5150..5905
                     /locus_tag="ACHAUN_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8460319.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACHAUN_001617"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACHAUN_001617"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8460320.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACHAUN_001618"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACHAUN_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8460321.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACHAUN_001619"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACHAUN_001619"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8460322.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACHAUN_001620"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACHAUN_001620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8460323.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACHAUN_001621"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACHAUN_001621"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8460324.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACHAUN_001622"
     CDS             10642..11184
                     /locus_tag="ACHAUN_001622"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8460325.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRMQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACHAUN_001623"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACHAUN_001623"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8460326.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACHAUN_001624"
     CDS             12777..13679
                     /locus_tag="ACHAUN_001624"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8460327.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACHAUN_001625"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACHAUN_001625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8460328.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACHAUN_001626"
     CDS             15122..15544
                     /locus_tag="ACHAUN_001626"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8460329.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACHAUN_001627"
     CDS             16013..16243
                     /locus_tag="ACHAUN_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8460330.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACHAUN_001628"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACHAUN_001628"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8460331.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACHAUN_001629"
     CDS             17654..17830
                     /locus_tag="ACHAUN_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8460332.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACHAUN_001630"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACHAUN_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8460333.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACHAUN_001631"
     CDS             18325..18708
                     /locus_tag="ACHAUN_001631"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8460334.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACHAUN_001632"
     CDS             18877..19761
                     /locus_tag="ACHAUN_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8460335.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACHAUN_001633"
     CDS             19875..20111
                     /locus_tag="ACHAUN_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8460336.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACHAUN_001634"
     tRNA            20199..20279
                     /locus_tag="ACHAUN_001634"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACHAUN_001635"
     tRNA            20286..20357
                     /locus_tag="ACHAUN_001635"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3566 c         4425 g         5611 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcatgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat ac
//
LOCUS       DAYYBG010000047        19694 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000047 DAYYBG010000000
VERSION     DAYYBG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.048"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACHAUN_001636"
     CDS             complement(<1..210)
                     /locus_tag="ACHAUN_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8460337.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="ACHAUN_001637"
     CDS             complement(216..446)
                     /locus_tag="ACHAUN_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460338.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="ACHAUN_001638"
     CDS             1063..1725
                     /locus_tag="ACHAUN_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460339.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="ACHAUN_001639"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="ACHAUN_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8460340.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="ACHAUN_001640"
     CDS             5145..5459
                     /locus_tag="ACHAUN_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8460341.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="ACHAUN_001641"
     CDS             5472..5846
                     /locus_tag="ACHAUN_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8460342.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="ACHAUN_001642"
     CDS             5856..6200
                     /locus_tag="ACHAUN_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8460343.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="ACHAUN_001643"
     CDS             6271..7623
                     /locus_tag="ACHAUN_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8460344.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="ACHAUN_001644"
     CDS             7683..8825
                     /locus_tag="ACHAUN_001644"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM8460345.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="ACHAUN_001645"
     CDS             8850..9104
                     /locus_tag="ACHAUN_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460346.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /gene="mobT"
                     /locus_tag="ACHAUN_001646"
     CDS             9360..10544
                     /gene="mobT"
                     /locus_tag="ACHAUN_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8460347.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="ACHAUN_001647"
     CDS             10541..10678
                     /locus_tag="ACHAUN_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8460348.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="ACHAUN_001648"
     CDS             10683..10904
                     /locus_tag="ACHAUN_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460349.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="ACHAUN_001649"
     CDS             10917..11417
                     /locus_tag="ACHAUN_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8460350.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="ACHAUN_001650"
     CDS             11514..13361
                     /locus_tag="ACHAUN_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8460351.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="ACHAUN_001651"
     CDS             13364..14260
                     /locus_tag="ACHAUN_001651"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8460352.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="ACHAUN_001652"
     CDS             14310..14699
                     /locus_tag="ACHAUN_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8460353.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16185
                     /locus_tag="ACHAUN_001653"
                     /pseudo
     CDS             14686..16185
                     /locus_tag="ACHAUN_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="ACHAUN_001654"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="ACHAUN_001654"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8460354.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18872..19694
                     /locus_tag="ACHAUN_001655"
                     /pseudo
     CDS             18872..19694
                     /locus_tag="ACHAUN_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DAYYBG010000048        19599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000048 DAYYBG010000000
VERSION     DAYYBG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.049"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..449
                     /locus_tag="ACHAUN_001656"
     CDS             132..449
                     /locus_tag="ACHAUN_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8460355.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(653..1018)
                     /locus_tag="ACHAUN_001657"
     CDS             complement(653..1018)
                     /locus_tag="ACHAUN_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460356.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1226..1708
                     /locus_tag="ACHAUN_001658"
     CDS             1226..1708
                     /locus_tag="ACHAUN_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460357.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1905..2795
                     /locus_tag="ACHAUN_001659"
     CDS             1905..2795
                     /locus_tag="ACHAUN_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8460358.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3120..5591
                     /locus_tag="ACHAUN_001660"
     CDS             3120..5591
                     /locus_tag="ACHAUN_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8460359.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5694..7430)
                     /locus_tag="ACHAUN_001661"
     CDS             complement(5694..7430)
                     /locus_tag="ACHAUN_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8460360.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7427..8131)
                     /locus_tag="ACHAUN_001662"
     CDS             complement(7427..8131)
                     /locus_tag="ACHAUN_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8460361.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8262..8765
                     /locus_tag="ACHAUN_001663"
     CDS             8262..8765
                     /locus_tag="ACHAUN_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8460362.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8725..9063
                     /locus_tag="ACHAUN_001664"
     CDS             8725..9063
                     /locus_tag="ACHAUN_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8460363.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9084..10502
                     /gene="ffh"
                     /locus_tag="ACHAUN_001665"
     CDS             9084..10502
                     /gene="ffh"
                     /locus_tag="ACHAUN_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8460364.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10614..11192
                     /locus_tag="ACHAUN_001666"
     CDS             10614..11192
                     /locus_tag="ACHAUN_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8460365.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11196..11717
                     /locus_tag="ACHAUN_001667"
     CDS             11196..11717
                     /locus_tag="ACHAUN_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8460366.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11796..12350)
                     /locus_tag="ACHAUN_001668"
     CDS             complement(11796..12350)
                     /locus_tag="ACHAUN_001668"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8460367.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12603..12878
                     /gene="rpsP"
                     /locus_tag="ACHAUN_001669"
     CDS             12603..12878
                     /gene="rpsP"
                     /locus_tag="ACHAUN_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8460368.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12890..13141
                     /locus_tag="ACHAUN_001670"
     CDS             12890..13141
                     /locus_tag="ACHAUN_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8460369.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13266..14057
                     /locus_tag="ACHAUN_001671"
     CDS             13266..14057
                     /locus_tag="ACHAUN_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460370.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14227..14748
                     /gene="rimM"
                     /locus_tag="ACHAUN_001672"
     CDS             14227..14748
                     /gene="rimM"
                     /locus_tag="ACHAUN_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8460371.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14738..15499
                     /gene="trmD"
                     /locus_tag="ACHAUN_001673"
     CDS             14738..15499
                     /gene="trmD"
                     /locus_tag="ACHAUN_001673"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8460372.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15635..15982
                     /gene="rplS"
                     /locus_tag="ACHAUN_001674"
     CDS             15635..15982
                     /gene="rplS"
                     /locus_tag="ACHAUN_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8460373.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(16114..17250)
                     /locus_tag="ACHAUN_001675"
     CDS             complement(16114..17250)
                     /locus_tag="ACHAUN_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8460374.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(17366..17932)
                     /locus_tag="ACHAUN_001676"
     CDS             complement(17366..17932)
                     /locus_tag="ACHAUN_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFM8460375.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(18068..18721)
                     /locus_tag="ACHAUN_001677"
     CDS             complement(18068..18721)
                     /locus_tag="ACHAUN_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460376.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(18771..19199)
                     /locus_tag="ACHAUN_001678"
     CDS             complement(18771..19199)
                     /locus_tag="ACHAUN_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8460377.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(19204..19578)
                     /locus_tag="ACHAUN_001679"
     CDS             complement(19204..19578)
                     /locus_tag="ACHAUN_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8460378.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
BASE COUNT         6325 a         3510 c         3842 g         5922 t
ORIGIN      
        1 gtaactatgc actgtaacga taatgctgct aaaatatctt tttacgaaaa acatcacaag
       61 gaaaaactta actatggtat tatctaaaca taattttttc ggtatttaat atttaattag
      121 gagggaatat tttgcttttt tatagtaatt ttatcttaat agtagcgata ttattacttc
      181 tgaatatatg gatttttgat aggagtagaa atgcaagtat aggttttaga actaaacgaa
      241 gtctgtcttc taaaaaaaat tgggtatact cacaaactat tttttatgga ggaattgttt
      301 taattagttt actttcttca acactttatt ctttgaatat tatagatgtt tccacctcaa
      361 attcaatatc tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt
      421 ttggagagaa gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatagtgat
      481 attaaaaatc agaaaagata gaaaaagcag gagatagata aaatgctgat atttcagcat
      541 tttttaagag tataaaagag aaaaaaagta ataaatgtat acatgatata agaaaaacat
      601 atatgtataa aaagaagaaa gtagcttctc tttgtacata tatggtttgt ttttatggat
      661 gccgagcttt tttataagat aaggctaccg cccataacga cccaaggatt tgtggaacta
      721 agtaaatacc tgcaagaagc aagatcagct gggtcggttt taaaggatga tagaagtaac
      781 gccattcagt aaaattggcg caaaataaat aggaagtgac agtagatata atattggcaa
      841 tgaataaaga tataatacgc ctgttccttt gaaaataata tccagcaaaa agagataaaa
      901 atatcaatgt gatgaacccg cttacggaat gataacgaaa atcaagcagt aaggcgaaat
      961 gcacataagg aacaaaagat aaaagcaacg gaaggttttt ccagcaagtt tttatcatct
     1021 ttcatcacca cctaacaagt gttattattt tgttagtttt attgtaaatt gaatcctgca
     1081 ataatcaatt actttttcat tatttctaga ttaaatgaat aaactctcat gccattatct
     1141 ctaaaaaaaa agtttttccc cctaattttt ttagatagaa aattgtatag tatgatgaga
     1201 gaaaaagtag tttgggggaa cgttaatgag aagaaaagca gtaatcgatc ggctttattt
     1261 atttttttgg ttttttccta tcgttttgct accttttaca tgggtaaatg ctgttgtaca
     1321 aggtgcggag atattaggcc tgatccaagg aagcggattt gtggtttcta ggaattttcc
     1381 agctttgact gttttctatt tagtgataag tgtaggtctc ttattaccaa aatctctgat
     1441 caatcactac aattggcagc tgctttactt ttcttgctta ttagtattta catctatgtt
     1501 ttctttgctg cctcgcttga ttatcggccc taatggtttg agcgagagtg gattgaagga
     1561 ttcagcttat tcctccatgt atttcttgac gatcaaacca tggttctatt tagtaattgc
     1621 ctcatttatt cttgcaacat ttttttactt ctggacagaa atcaggataa aaagtgaacg
     1681 atctgtatct ataaaaaata actattgatg gtggatcatc agtaaaaaag catatgagct
     1741 gcgtaaaatg catgtttctc atatgctttt tttattatta tgtaaagaat gcgaaaaacg
     1801 tttacatgat ttttacataa cttaactata tttttacagc aaccatacga gggatttaca
     1861 gtcgactgat aaactttagt tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt
     1921 ttactctagg tatgatggga attcttttag ccggatgcgg ctcaggatca ggaaatacag
     1981 atggctctgc cacacaaaat aattcaagtg actcaaatga acaagtaaaa atcgttgcgg
     2041 taggttcgac agcattacag ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa
     2101 atccaaatta tcaaatctct gtacaaggag gaggaagcgg aactggtttg agccaagtcg
     2161 aagctggagc agttactatc ggaaactctg atgtatttgc agaagaaaga gacggagttg
     2221 atgcttcaaa attagtggat cataaagtag cagtggtcgg gatggcgcca atcgtcaata
     2281 aagatacaga tgtcaaagat atcaccaaac aggaattgat tgatatcttt acaggaaaaa
     2341 tcactaactg gaaggaagtt ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa
     2401 acggcagcgg tacccgcgcg acattcgaaa aatggggact ggatggtgct acccctgtgc
     2461 agtcccaaga acaagattca tcaggaacag ttcggcagct ggtaagtcaa acaccaggag
     2521 caatcagcta tttagctttc tcatatctcg atgattccac acaagcacta agtatcgacg
     2581 gtgtagaacc aaaagaagag aatgtagcag ataacagctg gggaatctgg tcatatgaac
     2641 atatgtacac gaacggaaaa ccttcccctg aagtccaaaa attcttagac tacatgatga
     2701 cagaagaaat ccaagaagga cctgtcaaag aattgggcta tttaccaatc acaatgatgg
     2761 aagtagaacg tgatcacgaa ggaaatatca aataactagc agataaacat gccacggtag
     2821 tcgttactat taaggaaaaa tcaactatgt ttcccacata tacatttttc ctttaattgt
     2881 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattaa
     2941 acaccgttta ttcgaaaaaa agagctacga catagaactg tcgtagctct ttttttgcta
     3001 tatgacagga atgaaaggtg tttgcaagct taatgtgagg aacactttcc atatctgttc
     3061 tatactggat aggagaagtt tggaaatatc cagaaaacga aatcaatcga ggtagccaca
     3121 tgaagaataa atggataaat cgaagtaaaa aaaagaaagt aacacaaaga tcatcagata
     3181 aagtcatccg tctttttcca tcgtataaag aaggattaac ggcagatcaa gtcaaggaac
     3241 gaatagaaaa aggtgcagca aataattcag tcgatcccac attcaaaaca aatcagcaaa
     3301 tcgtattgga aaacatattt acgtatttca atttgatctt tcttatctta gccattttgc
     3361 tttgtttagt agaatcctat aagaacttga cttttttacc agttatcata gccaatacag
     3421 gaatcgccat ttttcaggaa attcgatcca aaaagatctt ggacgaacta aatgttttaa
     3481 atacagcaaa agtcaatgtc atacgggacg ggatagaaca aacaatagat atagaagacc
     3541 ttgtaataga tgacattgtt attttagaga ctggacatca aattccagca gatgcagagg
     3601 tcgtggaagg aaaactgcaa gttaatgagg ctttattgac aggcgaagca gatgaaataa
     3661 ccaaagaaat aggcgatcca ttaatgtccg gcagtttcat tgtttccgga aaagcttacg
     3721 cccgattaga caaagttgga gcagattcgt atatttccca gctgacagtc aaagccaaga
     3781 caatgggaga aggagaacag tcggagatga ttgcttctct taatcaattg atcaaatgga
     3841 ttgggatcat cattattcct atcggtatcc tgcttttttc tcaaagttac ttttttaatg
     3901 gaaacacgat caaagaaagt gtcgttgcaa tggaagcagc attgatcggt atgatacctg
     3961 aaggactgta cttgctgacg acgattgcat tagcattaag tgcgactaaa ctagcaaagc
     4021 aaagagttct tttacacaat atgaaaagca ttgagacatt ggcaagggta aatgtccttt
     4081 gtgtagataa aacaggaacg atcacagaaa ataaaatgtc tgttcaaaaa gtgatcgtct
     4141 ctaaaaaaca agaaataatc agtcagtcag atcaattgga agcatgcatt gctgactatg
     4201 caaaagcgat ggcaggagac aatgcaacaa tggaagctgt taaagcatat tttacaaaaa
     4261 ccactgatca atcctatcac catgttattc ctttttcttc tgtccaaaaa ttcagcagtg
     4321 tagcgttggc agacaaggtg tatgttttag gagcgcccga aatggttttg cgcgatcaat
     4381 tgccggaata tgcggaagag tttgtttctt tcgctgaaaa aggctatcgc gtgttggtct
     4441 ttggtgtgta cgatgggatt cttgaagaac cagtattgac agaagcagtc acaccattag
     4501 gctatattct gattgctaat cctatcagaa aagaagcaag agccacattt gattacttca
     4561 aaaaacagca agtagccatc aaagtgattt ctggagataa tccgctcacc gtatccaatg
     4621 tagctgtcca agcagggatt tcacatgctg atcagtacgt tgatgcatct aaattatcag
     4681 aagaagaata tgaagcagct gtcgaaaaat atacagtttt tggacgcgtc aagccagaac
     4741 aaaagaaaat cttcgttcaa ttgataaagc gtaacaatac agtagctatg acaggtgatg
     4801 gcgtgaatga tattctagcc atgaaagaag cagactgcag tatagcgatg gcttccggaa
     4861 atgaagccgc catgcaggcc tcacaagtgg tcttacttga ttctgacttt tcacgcatgc
     4921 cagaagttgt tgcagaagga cggcgcgttg tgaacaatat tgagcgatca gccagcttgt
     4981 ttttagtgaa gaatattttc tcctttttgc tctcactgtt ttcagtgatc tttgctctga
     5041 cctatccttt agaaccatca caaatcacat tgattagttt gtttactatc ggacttccat
     5101 ctttcttgct tgctctagaa gaaaacaaaa agcgaatcag agggaaattc atcatgaatg
     5161 tgatggaaaa agctgtgcca ggtggactaa cagatatgat cgtagtcggt gcgttggtga
     5221 tctgcggggt gactttgaat ctaaataaaa cagatgtttc tacagcatct accatgcttt
     5281 tgattgcagt agggttcctc gttctctata aaatctgttc accgctgaat aaattcagaa
     5341 gtcagatcat tcttttctgt gctagcggta actttttttc ggtcatcttt ttgcataagc
     5401 tgttttcaat caccggtatc tcagctgtat cacttttgct gttgagcatt ctatttttct
     5461 cggcagattc gatttttagg catttgacca cattggtaga atatttgttt gaacgtaaaa
     5521 atgaaaaaga gcaaaagccg tttagtcgcg tagctaaaaa atttctatcg atatttaaac
     5581 gagaaaattg acgaaaagta tctcaaaaaa gagtaaaaaa gaacaaccac tcgtgtatct
     5641 ttcgaagcag gatgagtcat ccaactttaa aacgcacgag cagttgctct tttttacatt
     5701 ttcggaatgg taacggtgaa agtcgaacct gttccaagat gactgtctac agtaatcgta
     5761 ccattaagga gttgcacgta gtcttttaca atcgctaacc ctaatccggt cccgcctgaa
     5821 tgtcggcttc gcgccttgtc tacgcggtaa aaacgctcaa agatccgttc ctgatcctca
     5881 atgtcgatac ctataccaaa gtcttggaca gaaaacgata gatcatctgt tgcctgatag
     5941 cggataataa tctcgctgtc gctttttgaa tattggacag cattttcaat cagatttttg
     6001 ataattggat aaaggatatc tgttttagta gtaaatggtt cgttctttcc aatcaatcga
     6061 atcgtcaacc gtttcttttc gatgattggc tgataatttt gaacgatttg ctggaaataa
     6121 ggttctgtat aaattgtctg gatctcgtaa gaattctcgc catcttttga cagctggatg
     6181 atttcccgaa tcagtttatc taaccggatc gcatcttttt gcatgatctc aagaaaagaa
     6241 gtcagagttt gcgggtcatc ttttgcacca tccagtaaag tctcggtaaa accaatcagt
     6301 gaagtcacag gagtttttaa ttcgtgagag acgttgccaa cgaaatcctt ttgtagcttt
     6361 tctaaacgtc gcactttcgt taagtcatac gccaccccta gaatctgtcc ggtaccttct
     6421 gaattagtaa agtagcgcaa actcaagtct aatgtctgcc gaccttctgt aagggtgatc
     6481 tcctgatgga tcagcggatg ctcaggagtc acttgatgaa tcagctgaat gagttttggc
     6541 tcttgaatga cttctgtgta ccgctgttca ggttgatagc ttaccacgtt taaatgtagc
     6601 tgcatctttg gattaagcag tagtaaacga gaatcagcat catcaatcag gaatacgcca
     6661 atcatcagtt cgttcagtaa cgtatataat tgctcttctg tggtagtaca cgcccgatac
     6721 atcttactca tttgttcgct caacgcatta atcgtctgat acaattcttc ccattgatcg
     6781 ggtgtttgca tgattacttc cgcctgatta ggattttgca ccattttttt tagtacgggc
     6841 agcacggttt tcaatggctg gtttttttga tagatcagat aatagatcat tcctgcaaga
     6901 acaaggaaga aagacagaaa gaagaaaaag acccaacggc gaaaactctc agttctcggt
     6961 aaaaaaccgc ttgtcggttc tgcgatacgt aaaatggctt ctaactggcc actttttttg
     7021 actggtaatg cgacatataa aagatcctca ttcaaagtag tacttttgcg caaagcgcta
     7081 cctaggttcc caccattcaa tacggctttg atttcgggac gggagtttcg ctgttcattc
     7141 aatgtttcat tgtttgtgtc aaataagatc tttccagtag catccattaa agtaatccgt
     7201 tcgttcgact gatggacgaa ttcttccaaa gcagttttgt tttgcgctgc ttggaggttt
     7261 tcgacatcta attgccggat cagcagttgt gctttttttg tcaggtagct ttcttgctgt
     7321 tccacgatct gctgttgaaa ataatgggaa atcaattgcc agccaccaaa aaacaaacta
     7381 actaaaagaa taagaaagaa gagacgttca taaatttttt tccatttcat cgttttggtt
     7441 cctgaaactt atagccaaat cctctaatag tcaccaagta ctttggatgt tttggatctc
     7501 gctcgatttt ttctcgcaaa tgactaacgt gtacatctac gattcggctt tgtcctgcga
     7561 agtcaaaatt ccaaatgcgg tctagcaatg tgtctcggtc gatcacacgg tcttttcgtt
     7621 tcatgaaata aacaagtaat tcaaattctt tcggtgttag ttcaatcaga tggtcgtcta
     7681 cggtcacttg ataattggtc aaatcagcag tgatttgtcc aatcgataga tattcaggct
     7741 cagcttcatc ttttgattgc ttacgaggtt caatccgtct gaatattgct ttcatacggg
     7801 ctaatacttc gcgaggacta aatggtttgg tcaaatagtc gtcagcacct atttctaggc
     7861 caatgatccg atccacttgg tcatctttag ccgtcagcat caaaatcggt gtttcatttt
     7921 tttcttgacg cagccgctga gtgatctcca tgccatccat cgttggaagc atcacatcta
     7981 aaatgatgaa atcaaatgat tgttctaatg ctaattctaa tccttcgcct ccatctgtgg
     8041 cactggttac cttgtagcct tctttttcca aattgaacgt caagagcgtt acaatagaag
     8101 gttcatcatc aacaactagt atttttttca ttcaaatctc ctttaaatag gtttcattct
     8161 gtttatattt tagcatagaa tcagtacaaa gggctatttt acaaaagaaa taaatggtat
     8221 aataggaagg cactttgctg aatagaaagg aagagcagtc aatgattgga atcagtgcat
     8281 gtttaggcgg tctggcctgt cgctatgatg gacagaaaaa agaagtacca gaattgatgg
     8341 aactagttga aactggagaa gctgtcatgg tctgtccaga agtgatcggc ggattaccaa
     8401 ttcctagaga tcccgctgaa atcattggtg gaaatggatt cgatgtgtgg aaagatcaag
     8461 caaaagtatg gacgacaagc ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca
     8521 aagcctatca aaaattgcag gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc
     8581 cttcatgcgg aacccattcg atttatgacg gaagtttttc cggaataaaa aaaaatggtg
     8641 ccggagtggc tactgcttat tttattcagc aaaaaatgac tgtgctctct gatgaagaat
     8701 ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat gaacgcgctc
     8761 tttgaatttt attcgacctt attgacagaa aaacaaatga actacatgga attatattat
     8821 gcagatgatt tttcattagg tgagatcgcg gaagaatacg aagtaagccg acaagcagta
     8881 tatgacaata tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa actccatctt
     8941 ttttctaatt atattgttcg agaacaggta ttggaaaata tgactgcata tattacagat
     9001 aagtacccgg aagataaaaa actacttgaa tatgtacagc aaattcaagc aatcgaagaa
     9061 taaataaaag gaatgaagaa aatatggcat ttgaaagctt aactgaacgt ctccagcagg
     9121 cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa gaaatgatgc
     9181 gggaaatccg ccttgcctta ctagaagccg atgttaactt gcaagtagtc aaagatttta
     9241 ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct ggaaagttta tcacctgcac
     9301 aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac acttggttct gaaacagcag
     9361 agttaaataa atcaccaaaa ataccaactg ttatcatgat ggctggtctt caaggggcag
     9421 gtaaaacgac ctttactggt aaattagcca attatttaaa gaaaaatgaa aatgctcgtc
     9481 cgctcctaat cgcaggggac gtatatcgac cagctgcgat cgaccaattg aaagtattag
     9541 gtcaacagct agacgttcct gtatttgata tgggaactga agtcagcccg gtagagatcg
     9601 tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga ctatgtattg atcgatacgg
     9661 caggacgtct gcatatcgat gaaacattga tggatgagtt gaaacaaatc aaagaattga
     9721 cgcaaccaaa tgagattttg cttgttgtcg atgccatgac tggtcaagat gccgtcaacg
     9781 tagctgatag cttcaaccag caattaggta ttacaggggt cgtcatcact aaacttgatg
     9841 gggatactcg aggcggggca gcactatcga tccgctcagt gacaggcgca ccaatcaaat
     9901 tcatcggttc tggtgaaaaa ttgactgact tagaagtatt ccacccagat cgtatggcta
     9961 gccgtatcct tggtatgggg gatatgctga cattgattga aaaagcgcag caagactacg
    10021 acgagaaaaa agcggaagaa cttgcacaga aaatgcggga aaattcattc gatttcaatg
    10081 actttattga acagcttgat caagtgatgg gtatgggccc actggaagat ctaatcaaaa
    10141 tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat ccaaaagatg
    10201 ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc agaaagagca aatcctgatc
    10261 tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc tggaaacagt gttgttgaag
    10321 tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa atgtccaaag
    10381 gggatatgaa catcccaggt atggaccaaa tgtttggttc aggcgtcaaa ggaaaactag
    10441 gcaaaatggc gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa aaacggaaat
    10501 aagatgaaaa aaacagcttc ggtacagact gaaaatgtct actgccggag cttttctatc
    10561 agcaattttt attctttact aatacaatta ctctaaacga agaggtggca cagatgagat
    10621 gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc tggggtgttc
    10681 ctgaatatga tgaccaaaag ctgttccgaa aattgatgct tgatattaat caagccggat
    10741 tgagctggca gacgatttta aataaaagtg aagcattcga tgaagcatat gatcgattcg
    10801 atatcaaaaa agtcgcaaat tatggagaaa caaaaatcag tgaactaatg gaaaacaaag
    10861 gaattattcg taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa gcagtcttga
    10921 aaattcaaga agaaatcggc agtttttcag agtatctttg gtcgttttct aatggaaaaa
    10981 tcgttgattc ttcttatcat tcacaagaag aagtcccaac aacaagtcct ctttcagatg
    11041 aattagcaaa agatctaaag aaaagaggat ttaaatttat cggaagcaca acaatctatg
    11101 cttttttaga agctgtcggg atcatcaatg atcaccttga cagttgtttc cgcaaagaag
    11161 aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg aaaaatcata ttttatggtg
    11221 ctataagttt agatggctat ttggcaacga aaacggatga tcttcaatgg ctttttgaca
    11281 ctccaacagg taagcaaact acttatgaag ctttttatga aacaatcgat acgaccatca
    11341 tgggcagaaa aacttatcag gaagcaaaaa aatatttgga gaccgaagca atttatccag
    11401 agaaaaagaa tttcgttttt tccacccacg cacaattaca attgccagat gctgaggtcg
    11461 taaacgaaga tcctgttgct tttctaaaaa atctacaaat gacagaagga aaagatatct
    11521 gggttgtggg tggcggtcga ttgatcaaac cgctattgga acatcaaatg attgatgaat
    11581 ggtatatcca gattacccct gtattattag gggacgggat tcctttattc caaaaaggaa
    11641 catatgaagc gcgttttgaa atgctagaca cgacacgatt cggtgaattt atcgaattgc
    11701 attatgtacg aaagtagaag taaataaaat aaaaaaggca actatatgca gaaatgtcct
    11761 gtacatagtt gcctttttta ttaccgttat gctttttact ctgttaattt gttagaaaaa
    11821 tctagatacg catctaaaat cgtttggaag aaacttaatg ttccttcaac gatattacca
    11881 ttttcatcaa ttagattcgc aacgtttccg atatatgctt ccggttgctg caaggttggc
    11941 acattcaaaa agacaagtga ttggcgtaga tggtggtttg cgccaaagcc gccgatccca
    12001 cctggtgatg cagtaacgac taaacctggt ttatgatccc aaacactgtg tccataaggt
    12061 cttgagccta catctaaagc atttttcaat actgctggca cagaacggtt gtattcagga
    12121 gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac gaaaacgagt ccattcagca
    12181 ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg gcagatcatc gatttttaca
    12241 aatacacttt cgtacccttc aggaagcaat ttcgcaaaag cttctgctac ttttttgttg
    12301 taagaatctt ttcttaaact accaataaaa aatccaattt tttttgtcat acgatttttc
    12361 ctccttcaaa ctatctataa taatcttaca aaagataagt aactggagca aataaaaaca
    12421 cttactgaca aaagctacat agaagataga ttacttattt ttaacaaaaa agaagtctaa
    12481 aatcttctat tttctaaaaa aaatcgaatt taatcaagct gtaaagaaaa aaagctttac
    12541 agggtaaaaa aaaactgtta cactacatct tgtgaataaa acaatggagg tggaaataaa
    12601 taatggcagt taaaatccgt ttaaaacgta tgggttctaa aaagagtccg ttttaccgta
    12661 tcgtcgtagc tgattcacgt tctcctcgtg atggacgttt catcgaaact gtaggtactt
    12721 acaatccttt gaaagaccct gcagaagtag ttttaaaaga agacttagtt cttgactggt
    12781 tatcaaaagg tgcacaacct tctgacactg tacgtaacat cctttcaaaa gaaggcgtta
    12841 tgcaaaaaca ccacgaagct aaattctcaa agaaataagg tgaccggata tgaaagattt
    12901 aagcgactta gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc aagtcaagtt
    12961 agacgtcgta gagtcgaaag acttttacga gtacaatcta acagtggctc ctgaagatat
    13021 tggccgtatc attggaaaac aagggcgtgt tgctaaagca atccgcacaa tcgtttacgg
    13081 tgttcggatc aatgcaccga aaaaagtgcg tttgaatatc atcgatgata aggaaaagta
    13141 aataaacgtg ctgtactcga attttcgagt gcagcttttt tgtttttttt attaattgaa
    13201 gaaatgatta tttggcaaaa caagcaagaa agcgtatcct tataggagga gggagtgaac
    13261 agccaatgag aaagaaaatg ttatttattc catttgctct attgttaagc gcttgtagtt
    13321 cggttactca agaatcagac aatacacatc tatcaagcag tatatctgaa actgttacca
    13381 gtccagaaaa aagtagtacc aaaacggctt ccaccactga acaaacaatg gctacctcaa
    13441 ataatgagaa aaagactgct ttagaccagt tgaaagaaca acagccaaat gttcccatgc
    13501 ctctagatgt accagtctct tcaggctatc tcaatatcgc agcgacacat acaaagcaag
    13561 gttattctat tttatattac agaacagatc gtcccctcgg gttgaatgct gacgaattga
    13621 atcaagaaac gcctattgca acctatctat atcaatatgg gtttgcttct agtcaagaaa
    13681 caatccaagt tttgcagcca tttgagatcg acacaaatgg tcagcaagtg gacttaggtt
    13741 ctcgaatcac agggtatcag caaggtgcag caggttcaag ttttcttgaa tggcaagaag
    13801 gaaactggcg tattcgaatc cgtgggaata atatagaagg ccaagatccc ttactattag
    13861 ctaaagaaat cgtagcttat ttggaagaaa acagtctacc agcacctgaa caatttggta
    13921 aaattactgt tgatatgggt gatacgacga atcgagccgt agaagtaagc tggcaagaac
    13981 cgaaaaatgc ctatacgatc actcatcaag atcctatgtc tgcgttgaag atggcagttt
    14041 ctatgaagag gttgtgaaat cagcgcgctg atctgagccc atcatttaac tccaagtcac
    14101 aggaagtaat caaaaatagc acgccgcatt tttgcataat tttggcttaa taccttaaaa
    14161 gaaaactaga attgcctctt cacacatgct aaaataaaac agacaaaaaa gaaatgggga
    14221 tataagttga ctgaatactt gaatgtagga aaaatcgtaa acacacaagg tatcaaaggc
    14281 gaagtccgtg ttatctcaac aacggatttc ccagaagaac gttacaaaaa aggaacggtg
    14341 ctaacactat tccaagacgg aaaagcacct gtggaattaa cagtcaaaag tcatcgcaaa
    14401 cataaaaatt ttgacttatt gagttttgaa ggacatcctt ctatcaatga tgtggaaaaa
    14461 tatcgggatg gcatattaaa agtgtcaaaa gacaactctg tagatctagc tgaaaatgaa
    14521 ttctattacc atgagatcat cggtctgcat gtattagaag atggaactga gttaggtaaa
    14581 gtaaaagaaa tcttgtcacc aggtgccaat gatgtttggg tcgttcaacg tccgaaaaaa
    14641 gctgatatat tgcttccata tatcgcttct gtcgtcaaag aagtcgattt agaagctgga
    14701 gtggtccgtg tagaaattcc agaaggactg attgacgatg aagattgatg tattgacttt
    14761 atttccaaga atgtttgaag gtcctatggg ggaatccatc atcggtaagg ctgtagataa
    14821 aggattatta gatatcaata tctctaactt ccgcgattat tcagataaca aacatcagac
    14881 agtagatgat tatccatacg gcggtggagc aggtatgctg ctcaaagtcc agcccatcta
    14941 tgataacatc caagccattg aaaaagctgc accggatgtc aaaaaaagag taattcttct
    15001 tgacccagct ggtaaacgat tcgatcagaa aatggcagaa gaattttcac aagaagaaca
    15061 cttagtattc atttgtggtc attatgaggg atatgatgaa cgtatccgca cattagtgac
    15121 agatgaagtt tccttaggag attatgtcct tactggggga gaattaggtg cgatggtcat
    15181 gatcgatgca acagttcggc ttttgccaga tgtgttaggg aatcagactt cagctcaaac
    15241 ggattcttat tcaaccggtt tattggaaca tccccaatat actcgcccgg cagaatataa
    15301 agggatgaaa gtaccagaag tattgacaaa tggaaatcat aaattgattg aagaatggca
    15361 attaaaagaa tcactaagaa ggacctatca acgtcggcct gatcttttgg aaacacttga
    15421 atggacacct caaatgacga aatttttaga agaaatcaag aaagaagaac aagaaaaaac
    15481 agcagatgaa gccaagtgac tttttctaaa atcttcttta cagtaggttg ataatatgtt
    15541 agtattttat ggtgagtgca aaagcactta actattacga tattccgctg tgagaaaagc
    15601 tcataagaat atttggaata aggagaagaa aaccatgaat ccattaatcg aagaattaac
    15661 aaaagaacaa ttacgttctg acatcccagc gttccgccct ggtgacactg tgcgtgttca
    15721 cgcaaaagtt gttgaaggta ctcgtgaacg tatccagtta tttgaaggtg ttgttatcaa
    15781 acgccgcggt gctggaatca gcgaaactta tacagtacgt aaagtttcta acggagtggg
    15841 cgttgaacgt acattcccat tgcatacacc acgtgttgca aaaatcgaag tagttcgcta
    15901 cggtaaagta cgtcgtgcaa aattgtacta cctacgtgca ttacacggaa aagctgctcg
    15961 tatcaaagaa atccgtcgtt aatcatcgtt atcagacaga gaaagaaccc ttggtattca
    16021 agggttcttt ttttattatc tgttttttta tattaacgat ttctatttgg tacatttttg
    16081 attaataaaa aggggcaaaa aaggggcaac aagttacagt ttttctatat cttctttcaa
    16141 tttttctttt actccttttg tcacatgcaa ataaattttt tcagtgatat cactattttc
    16201 gtgaccaacg cgatcctgaa tggcatacag aggagttcct agttccgcta attttgaaat
    16261 atgggtatgt ctaaaaatat gtgaactaag ttttttatca atcttcatgt cagctttatg
    16321 acttctcaaa taggtgttaa ttgctgttag ttggaaagga gttcctttcg ttgtttgaaa
    16381 tagaaattga ccatttggat ttaattctag aagctcattg taaatagcta tcgctttttt
    16441 tggtaaatca atttctcgca ttccggcagc tgtttttgta gaatcagatt ttttcatatc
    16501 agctattgaa cgttctctat acatcatcgt cccatttata actactgatg cattattatt
    16561 ggtaatgtgt acatcatctt tacttaatgc gattgcttca ccagacctca tcccagtcaa
    16621 atatagccac tggaaaagga gagaatatct tttattgtgt gaagttgtga aatctactag
    16681 tctattatat tcatcatctt ctaaaaattt atctttaatt tttatcgttt ttgactctct
    16741 tttataatca ataactactt catcgatagg atttttttct acataacctt ttttcatagc
    16801 atatgaaaaa agaagattca atttggattt aattacactg acgtacttat ttgacaagtc
    16861 atctttatat atcatgtctt caaaaatatt attcaaatct attgtattaa taccagaaac
    16921 gatgtaagtc tcaggtatct tcttctttat agtgtttaat atattattcg taggataata
    16981 tgtagattcc ttaacttgtc ttttgtatat aactaaccat tcttcaacaa gctcatgaaa
    17041 tgttagatca ggcttatgaa gtgttttttc gtttaatttt atatcaattt ttttatttaa
    17101 ctctagcaat gccactttct gagtttctcg agatttattt ttataagtaa tgctcacttt
    17161 ttttcttttt ctagtttttg gatcaatata tcgctcatta tatttatata ctttttttcc
    17221 gtttttatca gttttagttt cgatccacat tgttttcatc tcctatttgt tgctataata
    17281 ggaatagata agtaagccga ttatagcagg tttatttttc atcacgtcca caaactttgg
    17341 cgagggaggg gcgtgttttt gttagttatt gtggtaggtt atctaaagcg tattgtgctt
    17401 gctcatttgt aaaaccttca aatataagtt gatcgtacaa ttgcgaatct gacattgatg
    17461 cccaatcatc gtaactttcc gctttagcca aagcttgttc gttgtaatcg acatcaacat
    17521 tagccagcgc ataatcgatt gcatcacttg gatattcttc aaactctagt tgttcacgca
    17581 aaccttgttc agacatacca gcccaaccta gatagctttc agctttgctc aatgcatttc
    17641 tgtattcacg aggaacactt tcttcttgag taacttcttc tatagtagtt tcagtagttt
    17701 cagtttcttc ttcaacgact gtagattctg ctaccgattc tgcagaagac acttctgtca
    17761 cgctttcttt agttgtttca gcgacggtat ttttagcatc atcactgcct ccttgcgaac
    17821 ctatagcaat aaacaccaca acaaccgcta acaaccaaaa ccatacccgt ttataaaacg
    17881 gtttcttaac tttatacatt ttcccatctt gacccataac tttttttgcc atttaaatat
    17941 ccctcgcttc ttgttataat atatttgtga tctcagaaat gaggtatgag tccgtgttgc
    18001 agcacgggct tttttcttta taactttttt agagattata ggcaaaatag tagggcataa
    18061 aaatgtatta ttgaattccg tatttagaaa atcctaattg aacttcaccg gaagtctttt
    18121 gctgtgttgt acgcaatgct tcttcttcag acattccatt ctgtactttc catgcaacag
    18181 gcgacatccc gtatttgtta acaaaatcag taagtgataa agtgtcagcg tcttgctgag
    18241 cgcttgtttg ttggtcttct ggattctgtt gagatgctgc ttgttgttct ttctgatctt
    18301 gactgataat attgccagca tcatctgtag tcaatccatt ttcataaagg gccacgccga
    18361 aagcttccca ctctttgttg gaccaatttg cacgatcagc tggagttgac tgtaaagtgc
    18421 gttgtttcat ctgttcatat gtttcttctt gaggtgcggt ttggattgta ctctgactgg
    18481 agcttataac tgttgggcta ggttccgccg tagcttggtt ggagcttgta actgttgaac
    18541 tagaatctgt tttagatgta gatttgctag tagatgaact ggtttcagtt gtttcttttg
    18601 ttttacttat ttttgtttct tggttagaag tggtatctgt tgattcagct tttttattat
    18661 ttgaacaagc tgaaagtagc agagcagtac ttaacaacaa cataacgcta acttttttca
    18721 ttttataatt cctctttctc gttgtaatat gtgtgctaac acgggctttt ttatataaga
    18781 aaacgataag cgctttctgg aagcccgtaa agattcttta attcctcgat ttttttagga
    18841 tattgatcat tatcttcttt ataaagagaa acaatgagat tagcagcaaa gcaattagct
    18901 tcgctttcag atttgcttct agatgttctt gttgatacat aatagctgga taagccacga
    18961 tgaaaaatag cgtgacctaa ttcgtgagcg caaatgtaga atctttcctc agagtctcgc
    19021 agttcatcat ttaaaaagat tatcgcacga tctctaattt cttgaaactg ccccttggga
    19081 ttctcgataa aaggaacgta ttgaatttta atgcccatct tttcacaaat ataaaaagga
    19141 ttagcggact ggtatttccg cttcaactcc tcgactaaat taatcgtatc catctccata
    19201 agctcacatc tttttgcctt tttctttgtc ttctttcaca atatcccaga aagtcgctat
    19261 caggatatct tttacgcgct gtatttgttc gggtgtcaat gtttccccac cataagacat
    19321 attaacattt gagtctagta gtttatcaag ttcaaccact tcctcttttg tagcccactt
    19381 gggaacatta ttatttccca ataaataatc agttgtgaca ccaaaataat cagcaacttt
    19441 ctttaagttc tcagattttg gcgaggcttt atcccatctt cttatttgtc cattagagat
    19501 gcccacctgt ctttctactt ctgctatagt cacatgcttt tcgtctgcta attctttaat
    19561 cttagtaact aaactcatta ttatcaacct ttcaaagct
//
LOCUS       DAYYBG010000049        19457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000049 DAYYBG010000000
VERSION     DAYYBG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.050"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACHAUN_001680"
     CDS             133..3660
                     /locus_tag="ACHAUN_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8460379.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACHAUN_001681"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACHAUN_001681"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8460380.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACHAUN_001682"
     CDS             7448..7789
                     /locus_tag="ACHAUN_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8460381.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACHAUN_001683"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACHAUN_001683"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8460382.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACHAUN_001684"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACHAUN_001684"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8460383.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHAUN_001685"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHAUN_001685"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8460384.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACHAUN_001686"
     CDS             13000..13737
                     /locus_tag="ACHAUN_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8460385.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACHAUN_001687"
     CDS             13751..14566
                     /locus_tag="ACHAUN_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8460386.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACHAUN_001688"
     CDS             14579..15226
                     /locus_tag="ACHAUN_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8460387.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACHAUN_001689"
     CDS             15242..15898
                     /locus_tag="ACHAUN_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8460388.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACHAUN_001690"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACHAUN_001690"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8460389.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACHAUN_001691"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACHAUN_001691"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8460390.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACHAUN_001692"
     CDS             18240..18758
                     /locus_tag="ACHAUN_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8460391.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHAUN_001693"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHAUN_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8460392.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagc
//
LOCUS       DAYYBG010000050        19078 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000050 DAYYBG010000000
VERSION     DAYYBG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.051"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACHAUN_001694"
     CDS             6..245
                     /locus_tag="ACHAUN_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460393.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACHAUN_001695"
     CDS             312..521
                     /locus_tag="ACHAUN_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460394.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACHAUN_001696"
     CDS             581..1252
                     /locus_tag="ACHAUN_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8460395.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACHAUN_001697"
     CDS             1305..3281
                     /locus_tag="ACHAUN_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8460396.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACHAUN_001698"
     CDS             3294..4049
                     /locus_tag="ACHAUN_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8460397.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACHAUN_001699"
     CDS             4046..4870
                     /locus_tag="ACHAUN_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8460398.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACHAUN_001700"
     CDS             4880..5128
                     /locus_tag="ACHAUN_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460399.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACHAUN_001701"
     CDS             5171..7270
                     /locus_tag="ACHAUN_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFM8460400.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACHAUN_001702"
     CDS             complement(7308..9365)
                     /locus_tag="ACHAUN_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFM8460401.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACHAUN_001703"
     CDS             complement(9479..9691)
                     /locus_tag="ACHAUN_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460402.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACHAUN_001704"
     CDS             9878..10345
                     /locus_tag="ACHAUN_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460403.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACHAUN_001705"
     CDS             10342..10584
                     /locus_tag="ACHAUN_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFM8460404.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACHAUN_001706"
     CDS             10602..10904
                     /locus_tag="ACHAUN_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460405.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACHAUN_001707"
     CDS             10976..13030
                     /locus_tag="ACHAUN_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460406.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACHAUN_001708"
     CDS             13030..15642
                     /locus_tag="ACHAUN_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8460407.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACHAUN_001709"
     CDS             15639..18170
                     /locus_tag="ACHAUN_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8460408.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="ACHAUN_001710"
     CDS             18222..18554
                     /locus_tag="ACHAUN_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460409.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..>19078
                     /locus_tag="ACHAUN_001711"
     CDS             18555..>19078
                     /locus_tag="ACHAUN_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460410.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILG"
BASE COUNT         6854 a         2966 c         3803 g         5455 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgatccta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    13141 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    13201 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    13261 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    13321 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    13381 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagg
//
LOCUS       DAYYBG010000051        18706 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000051 DAYYBG010000000
VERSION     DAYYBG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.052"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..1003)
                     /locus_tag="ACHAUN_001712"
     CDS             complement(92..1003)
                     /locus_tag="ACHAUN_001712"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8460411.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1380..2018)
                     /locus_tag="ACHAUN_001713"
     CDS             complement(1380..2018)
                     /locus_tag="ACHAUN_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460412.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2022..4004)
                     /locus_tag="ACHAUN_001714"
                     /pseudo
     CDS             complement(2022..4004)
                     /locus_tag="ACHAUN_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4184..4849
                     /locus_tag="ACHAUN_001715"
     CDS             4184..4849
                     /locus_tag="ACHAUN_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8460413.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5246..7279)
                     /gene="brxL"
                     /locus_tag="ACHAUN_001716"
     CDS             complement(5246..7279)
                     /gene="brxL"
                     /locus_tag="ACHAUN_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFM8460414.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7397..9919)
                     /gene="pglZ"
                     /locus_tag="ACHAUN_001717"
     CDS             complement(7397..9919)
                     /gene="pglZ"
                     /locus_tag="ACHAUN_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFM8460415.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10035..13667)
                     /gene="pglX"
                     /locus_tag="ACHAUN_001718"
     CDS             complement(10035..13667)
                     /gene="pglX"
                     /locus_tag="ACHAUN_001718"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFM8460416.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13731..17402)
                     /gene="brxC"
                     /locus_tag="ACHAUN_001719"
     CDS             complement(13731..17402)
                     /gene="brxC"
                     /locus_tag="ACHAUN_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFM8460417.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17427..18002)
                     /locus_tag="ACHAUN_001720"
     CDS             complement(17427..18002)
                     /locus_tag="ACHAUN_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFM8460418.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(18002..18601)
                     /locus_tag="ACHAUN_001721"
     CDS             complement(18002..18601)
                     /locus_tag="ACHAUN_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFM8460419.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5768 a         3566 c         2618 g         6754 t
ORIGIN      
        1 taattaaact actgttgatt tagttctaat aaaaagattt agatgaacag ttttattgat
       61 ttaaatagtt catatttttt atttataccg cttatttact acttgctgta ctagaatctg
      121 aagtgaatgt actcaaaaca ttttcaaagt ctttgtcttt gatttttaca ttagcgtctt
      181 tcaatacttc gccaattact ttattctgga aggtactgtc acttaatttt gtgtctgttg
      241 caatttcttt caattctttt ttgtatttat ccatgtcgtt gcctttgttt tggtttttca
      301 ccattttaac cacataatat tccgtagtat aagttgaagc attggtagaa gtgatcacat
      361 cagaaacttg accatctttc aacttaaagg ctgcttcttt gacttctgcc ggaaccgttg
      421 tagtagtgga atcaaatttc acttttccgc catcttcttt tgtcgtagta tcagttgact
      481 tgtctttggc taatttagag aaatcgtctc ccttatcagc agctttcttc acatcttttg
      541 catcatcttc acttgctact tggataattt gagcttccac ttctggatgg aaagattccc
      601 aagccgtttt taaatcttca tcggtcaatt tgatatgttt ctttagacct tcttgaaagg
      661 ctagactttg tttgatactg tctttgaatg tttgttctgt gtaacctgca gatttcaatt
      721 gtgaatcaaa tgaatcaccc aattgtttct ttgtttgatc atattgggaa tcgatttctt
      781 tggttgatac gtcatctcca tatttatctt caaatacttg ataaacgatc aactgttgca
      841 cagcggaatc tgctccttgc atttttttca agctatccat tacttcttgt tcgctgattg
      901 taccgccttt ataactaacg gctgtttttt caccggcatt cgtacagcct gacaataagc
      961 cagtgcctag tacgatggca agtgctaaaa atcttttttt catttatcaa ttctcctact
     1021 tttcgttttt tgcgaattgc taaaaacaaa ggtgttcaag atgaaaatca tcaagaacac
     1081 ctttactaat ctgctcgata cgtcctgaat agatataagg ctacacaatc cagaaagttc
     1141 gctcagcaga ataaatgtca agtagcacat cacaccttaa tatactactt cttttgttac
     1201 cgtttttcaa gtctatcttg cgttccgtat ttaacgtaac taattcttag aacaaatgtt
     1261 atcgattttg tttttcttct tccgctgttg ggatattcaa atcagacact tttatgttcg
     1321 attttccaga catttttttg ttgtttttat tagaaaaagt ttgttttttc atttgttttc
     1381 taaagcatga tcttcttgtt tcctattttg gtcagatatg gatcatgcgt gaccacgata
     1441 actaacgtac cttgggaagc ctgttgcgat aagatttcaa cgacttgctc cttatttcgt
     1501 ttatctaatg atccagttgg ttcatcggct aaaatgattt tgctattctt caataagact
     1561 ctcgcacagg cgattctttg cttctcacca ccagataatg tgtagatagg cgtacttagc
     1621 ttcttgttta tatttacttt tttcaatgcc tcatactttg ctgttcgctt atctgaaaca
     1681 tttaaattcg aaaacttcaa tccaatatct aaattaaaat cgattgtttc atattcaatc
     1741 aacccaaaat cttgaaaaag aaaagagagg gcatttcttt ttatcaagcg cttctctctt
     1801 ccagttaaat gttttgtttc ttgattaaat aaataaaatt gccctgaact gaattgatca
     1861 ttcaatccta atatattcaa taaagtactt ttccctgctc cgctctctcc aactacagtc
     1921 acaaaatccc cagcatccgc agtgaaacga tccaaatgaa gaatcgtttt gtttcgaatg
     1981 atttttttcg caccttttat cactatcaac ggctccaatt gttaacctcc attcatcagc
     2041 aataactgac ttttcttttc tgtagacaaa ataaaaacaa ctgaaagaat aaaatcaatg
     2101 aagctaaaga aaaaaatatt aaaaagatat ttaggctctc tttctataag aaaaaggaag
     2161 attacacttg tcgttcccca tttgatgagg ttcatcatga agaaatcttt atacgtttca
     2221 aagagcgaat aaccacttga acgaaagata gcgattttcc gacaattgta gacaaaatat
     2281 atgcataaca tgacatagct caacaagaag aaagaaaaga aagtgagtaa aaatatctgt
     2341 atcaagtaat gaaatgatct gcttaacact ttgacagcta tctcttcagt ctgagtatac
     2401 ggaaatgata actgcaagtt atcacgtaac tgactttctt caatcagctc aaataacctt
     2461 tctttcgaca aagagcctat cttgatcttt aacggtggat agatttctcc gctgagaata
     2521 tttctttccc aatacgtact gtttttcaat gtcagtattt caacaaccgg ataatgttcg
     2581 atccagggga tagaaggaat aaatgtataa atgggttgat tgttttttat aaatagaaat
     2641 ttcggttctt tttcactgat ttcttgataa tattctgtta tttgcgccag gtcatttttt
     2701 aaatctgtat gtctatatcg ttcagggatc aatataacag ggtgcgtttc tttttcggtt
     2761 atttggatca accttccaga gacatcaatc aaaggaaatt tcttaagata attcagattt
     2821 atttttatcc ctctgccaaa ctgttcattt tcttcattca agtaatctga tacattgact
     2881 aatatacttc cgtttttatt caaagtttca tataattcct gatcggctgc tgatgcaaaa
     2941 cgattggaat aaataaaatc cacgtggttt ttcccgatgg ttactggata aaatacatga
     3001 tagtcctcac cagcgatttc ttttggtgtt tcatgggaaa cagctaactg tgaagaagaa
     3061 aaaacgatag ttgctaattc gacattggca acggtcaaaa gaagagaaaa caacaatcca
     3121 aatgtaaaaa gaatgtattg gatatagttg aacgcttctc ttaacgaagc gattttttca
     3181 actaataaaa gagaaatcaa agagataccc tatattccgc tgaacaagaa aacgtacatc
     3241 tggaacaaat aaagaaattg ctccttataa tacataaaaa taaaaccacc aatgattcct
     3301 gcaattatga atgtaataat gattttcttt ttccccaata aacaccacaa tacttgatgt
     3361 aatgaaaagc cattattcaa caataaactg atttctttat tcatcaataa gaaaaaggat
     3421 gataacaaaa ggaataaaag aatcaatcca atagttaaat acactcgata attatatacg
     3481 tctgtacttg aaataaccga atacgaatag ctattttcaa agtcttcaaa aatattagtg
     3541 gcataatcag tgtgaaatgc ttgattgaat cgttccacaa atagtaaaat gaaccgttca
     3601 taggcttctt tttctctcgt catcaagaaa aattcacctt caaaatcttt tattttgatc
     3661 gattcttcaa tggacgttac gcgaaaggag gtattagaat agggtacctt ctcgtgagaa
     3721 gatttgacat ccccttgata atacaatgtc atttcaagct ttggctgtat cttgaagttc
     3781 caagatgtat ctacttcatt ggattcattt actgtttttt tcatataaga aagctcagtt
     3841 tctgatgaga cattttcaaa taccatgatt agctgctcat attcttcttg cccaataaaa
     3901 tgttgtggca atcgaatcgg agtataagta tctgtttgat ggaaaaattc atttgtccga
     3961 tagagtacct gtttatttat aataaccaag gcaaaaccac tcatgatacc ggtcactatg
     4021 attgtgagta aaactgtgaa cttttttttg attatcacca ctctccctta cttaaatgat
     4081 tctttgataa aatcataccg tttttcatca aaaaaacgta tctatcaaat gtataggtca
     4141 tgtagaataa atctatatac tgtagaatcg aaaggagaag aagatgaaca tcggagaaaa
     4201 aatacgaact taccgcagaa attgtaagct tacgcaaaaa gaattaggcg aaaaaatcgg
     4261 tgtttccgat aaaacaatct ctagttggga aaattctcgt acaatgccag accttgaaat
     4321 gctgtcactt ttacatcaga ctttaggtct gccgattgat tttccttcta ttgaatcagc
     4381 taccgaatca atagaaccaa cattgccgga tgaacctctt gttatcaaga aatcttattc
     4441 atcaaaacaa cccttctcac tcaaaaagac actatctttc tttctgctcg tcttttgttt
     4501 ggtctatctt tttcttatca attatcaata taaaaatgaa acggttgatc gattcaatcc
     4561 ttttttgaaa ccacaaatcg gctataccac actccctgta ggatacgtgg aaaattccat
     4621 cgaacattgg gtatgtaatg atatctttgt tcatggtaca tggctgtctt ttcacgctgg
     4681 agaggtagaa aaaggtaaga atcatgctgt aatcaaacac aaaggaaaac acgtcatgaa
     4741 cgttcgcctg ctcacttggg aagccattcc ctcagattac caagaaatcc tcatccaaga
     4801 ctatgacgat gccttaaaaa gaagccctca ggagccagat aggcattaag taggagatga
     4861 acgtagagat agaaaaaatc aatgcacatg atcttatcaa aaaaaaacag gggtacaaat
     4921 taattgaaaa agctttgaga tgaaagttcc taaataaaaa agttcagaat gataggtaaa
     4981 aaaatgacta actattctag tattaggcta tttgtattat tcttctgtta tttttatatc
     5041 aaaaaatcca gaaataacta aatataggta tttctggctt atagtataat atttacgcca
     5101 cagcccccaa tgattctttt tggaatcatg atcaaggacg gacatacaca aaaatatttt
     5161 gcccattcga tccgaataga ttaacgacta atctagtaag taatatagat tggtacgtag
     5221 tcaaaccagc aaatggaaaa gttgtctatt ccaccccaag ggctttaaag acagcatctt
     5281 ccggcgtttg ataaaaaatc aaattaaagg tacccattaa ttctgggggt acagttccaa
     5341 tatcagcagc gcttatcata ggtacaagta cttttttcgc tccggcatct tttgctactt
     5401 gtaaacagtt agctaaatta tctaccttaa tcatagaacc accaattgta atatccccta
     5461 aaataactaa acttggtaat acaggtttat ttaaggaaac tgagcataaa gctattatag
     5521 ttgataacga taatgttttt gtcataccaa tgccattcat atcttgaaca tgaacgacaa
     5581 aatcttttgt cgtcaaacta atagactgac taacacttcc ataattagct cttaaatatc
     5641 ggtatgccga atccatcgct tctcttgctc cagcatctga attgatccca gtacgttcaa
     5701 actttccatt tcccgaagtc atttgcgttt ccaatttata ggtacctaat tgcccagttt
     5761 tgccataaga taccgtgtac aaatgccctg gcttccctaa cccttctgga atcaaagttc
     5821 ctccaccttg ttcaggaaca gaaacatact cttctcgctc ttcttctaaa tcaatgtatg
     5881 aaaaattgac atcataaaat tccattccac caattttctt taattgttct tttacacgac
     5941 gacgcatttc aagagcaaat cttagtattt cttcgacatc acttttttca aatctaccat
     6001 caggatacaa cagtttcatc atcccagata caatccgacg aactgcaatc gtatcccgct
     6061 gatttaattg agaaccaagt ttgaaatatt tatcaaaact atcgccaaaa ctaacttttc
     6121 gaagctcacg catgacttca gaaaaataat ctgtaatgaa gccatagtca tttgtaaaaa
     6181 aatctggtcg atatttagga atttcccaac ctggtaaata acaatgcatc cgatccaaaa
     6241 aggctgaatc cgtccccata acctccggaa atggctcaaa taaattcgaa gttttcaata
     6301 ttacatcaac gctttgatta atatttccta caaaagccat tgatgctgaa gcattctttt
     6361 cttctttgcc tctagcaaaa gatcctgaag ccatgtaatc tttcatgatt tgaacgccat
     6421 cattatcctt aaatttaatc ccagcaactt cgtcaaaagc aacacagtcc cacataccta
     6481 caagtcccat tgtacgacta gccatattat agaaaaggtt tgctactgtt gtttgtccac
     6541 ctgaaacaag gattgagttt ggtgaaattt ctttatatac atgcgattta cctgtaccac
     6601 gtggtcctag ttcacagaga ttaaaattat tttcaattaa tggaataagt cttgctaaat
     6661 gaagccattt taccctttta ttaaattgtg taggttccat accaatggac cgtagaagta
     6721 agtcaatcca ttcatttgta gtaaactcct tgcgagccat cttgtaagcc tcaatgtcta
     6781 aaccaggcat ttggattgga tcaacctttt ttgcaataaa agggctattc caacgatcat
     6841 tctcatcata ccaatattcc atcgaaacaa tacaccagat ccccccacct aataatcgat
     6901 caaatttttt cggatactct tcactaattt gaacattttt taatcccagg ttagagaatt
     6961 ctgcttcata acaatctttc ttttcattta aatgtacagt tactttatca ataactgtat
     7021 aaccacccaa ttcacgaatt tttgctttta ctttttctgc ttcatcagga cgcacatagt
     7081 ttcgagcaag aatattttta acattttcca cacctttttc aaccaatgtt tcatccaatg
     7141 acgaagcata cataccaagt aaatattcca atacataaac aggtacattc gcaccttctt
     7201 taattttctt tgttaaatct tttctgacaa tctttccggg aaagaattca gataacttta
     7261 aaatttcctg ttgttccact agaattcctc catgtgttca gtcattataa tagcaattaa
     7321 agtatatcat acttaaccag taggcttgat aatacaaaag aaaaagtatc cacaaaaaat
     7381 tgtggatacc tcgtttttat atatcaaaat caaatgattc tgcaaaagga ttatctataa
     7441 tgaattttat tcgctcggtc aaatcatttt tttgattttt tatcactaaa taataagtat
     7501 cttcaactgt atatcgacga tttacaaatt caaaaacaaa tttagtaaac cgctgatttg
     7561 catcttctgt ttttgaatcc gcaacataga tttcttcatt tgagatacgt tcaccatatt
     7621 catcttcaaa ataaagccag aaagatgcgg ggtcataaac atcactaatt gcattttttt
     7681 gatagaattc taacgtggtt gataatcctg taattctgcg gttgctagtc attaattcaa
     7741 ttgctactgg ttcctttgca ttgctacctt tggctaccgt aatttctaga acagggacta
     7801 gcatttcttg aatagatgac cctccatgaa cgtagctttg tcccccgcca gctttcttaa
     7861 aaatacttga agtaatcggg acacaaatgt ttctagtgtc atcatttcct aaagaatctc
     7921 ccaaagagta atttttcgta ccaattattt cgtattcttc ttcactaaga ataaaacgac
     7981 gttctacacg atctttttca tttctagatg gattttcaat cttatctact tcttcaacgt
     8041 ggcttctttt ataaataaat ccatgatcag ccgtaactat aaaacgatag atactgccat
     8101 cattatgcaa gcgattaata agtcgggaaa tttcgcttat cgctaaatca caggcctcaa
     8161 aaactcgatt ttcattcgag tgatcacctg ttctatccac ttgatcgtga taaatataaa
     8221 tgactgattt gccattaaat acttctcgta cctcagcctt attcatctta accacatcat
     8281 cataatttaa agctacagca tttgcatctc gttgctggag aatcgcattt cgattggata
     8341 aaccattcgt ttttttccca tcaatcagta cttctgttcc ttcattgtaa gttagttgat
     8401 tgtttggtaa taaggcggct ttaccgaatt cagtaactga aggtaataca gaaagaaagc
     8461 tagccatgtt tgttgaatac ttcttttctc taactaactt acgttctaaa ttcatcccta
     8521 actcatatcg aaaagcatct gaaataataa ctacagtttt aacagagcgt ttcattacag
     8581 cttgattata aaattttcga atggatggac gattcttaaa ttcaaaagtt tgattccaaa
     8641 tttgtcctga ttgatcaaga aacttcgaat aatttgcttc aactaatcga tacaattggc
     8701 catatttttg cttttcttct atatgatcac tattccaaat aaatttccga taatgcgtat
     8761 caacccaata catttccttc tcatattgag ttagcaaatt atccatgttc atcggcaagc
     8821 acaatctgtt tttatccaca agataataag catgtttcaa taattgatac tcataaatat
     8881 aatccgatgc gaaatgcatt ttttcgcgct gctttaaact atcaaaaatt gtttgtcctg
     8941 caattttcgt tgtagtgtca gaagccttta atcgttctgc ataaaacatt aatatttttg
     9001 catgaatctc agaaaatacg tctgcttcaa ttagatcatc aatttctata ctgtctaata
     9061 atccatcacc atcaatagcc tgatatactt ttttagatag ctgttcaaag ctttccatgt
     9121 aacgtgtatc attcataaac tgatccatga aagtaataat gtttgctttc aaatgtagta
     9181 tttcaaatcc tttgaacttc ttcggcatct cttgatcaat ttgataataa aaatagttca
     9241 aataaagaca catcgtcagc ttttccaatg ttggctggct atccgtaaag ccaaatttca
     9301 tttgaataaa ttcccaaaat ttagctgtta actgatactt atcaagttcc cttaaaggat
     9361 tctccctact ttctgtaaag ctttttagaa ctacacgcag taaatcattt aatcgaatac
     9421 ttttcgattt caacaaaaca gccattatca ttaattcaat cgtttctgat ttattaattg
     9481 tcatatgcgt actaaattca ccaaaacttc tcatgcgatc ctttgcatta aagaaattac
     9541 gatactgttt aactgtctga tgaaatctaa gtggaatagc caattcattc atgataatcg
     9601 ctaagcggtc tgcactaaat tcctctgaat aattcaatac actcaacaaa tgattatctt
     9661 catctttatc atcccaacga ggaaatggcg catagattaa atagttagtt tcgggatctt
     9721 catattccaa taaacgctta gttttaaatt gtgcgtcctt ttctaatcga tagatttttg
     9781 cattttgaat gaccaatgag tcaatttctt caagaaaatc ctgatttgaa tcaaaccaaa
     9841 aaatgatttt cctacatcca taattaggaa aacgttgatt taatttttct tcaatttgtt
     9901 gcgtatttat atctgtcatc aatctcacct aatcgttctc tttctatacc ccttatattg
     9961 acataatttg gataatttcg cacgcaattt aagaataaaa aagaaagttg ttagaaaaca
    10021 actttcttag ataactattt ttttggttct ttgacccctt ttgcaaaaat ttggaatttc
    10081 tcgccggttt taggatcacg ctgcaactta tcataattga caattacacc atcatctaag
    10141 tctaattcaa ttcgttttaa tgctaagtga cccaacttct cgtcatactc acgaacttct
    10201 tttaattgct tctgaatctt tacaatttct tttgtcattg catttctttc ttttgggtta
    10261 tcacttgttg aaatttgttg ctgacgcaat tcgattcgat tgtcatacgc tttttgtagt
    10321 tcgtgtaggt attcagtacg tacttttcct aattgatctg gtgtatagcg atgcaagtac
    10381 attaacgctt taaagccatt ttgttttcct gagtccaatt gccaataaat tggtcgattg
    10441 gagtatgttt ttaaatggtc tttgtaaaaa tcttgtacga agtaacgacg aataatttct
    10501 cgacttgtcc ctttgccacc taaagtgtca gcgataaact ggagattttc ttcaagggtt
    10561 tctttaccaa aagtaatctc aacgaaatca atgaaacgat tgacaatgtc aatttggcta
    10621 tccttgaaat actcatcatc ggtgatgagt aaaacattat cagtatcagg tttaaatttt
    10681 tgatagcggg aaggatccca ttcgccgcca gcgaagatga gaccatcttc gtctagggag
    10741 taacgaccga agatacaacc tattgaataa gaaataaaat ccttaatttc cctcgcctta
    10801 tcaggtaacc ttgcagagat atattcatct tgaacctctt ttgttaattc tttttcaaga
    10861 gaataatgat taatgaatat gtcgttcagt tcctcttcat tcgattttag agtattaaat
    10921 tgattaacca actgcctact tagacagtta aatgcctctg agagatcttt tttaaacata
    10981 taacttaaca aaatgtttct tctaaactcg tatgacgttt caagagcatt atagtctttt
    11041 ctagctatat cgatattttc atccactttt tctcttattt tttttaattt tctctcagat
    11101 gtaactttaa gaaaaggtag ttttcccatt ggtccttcat ggtaatccaa ggtgggagaa
    11161 ataaaagtta aaaagtgatt aactaattta gagtttacat agccaaatat ttcttcaaag
    11221 tcagttatat cctttgagaa cagaacagat cctttagttt caaaaattgc ctgattctgc
    11281 aatctaaaag atacttgtcc agatgaaagt gtcgaccaag taattccctt tttaaaatag
    11341 taattaagat tttgtggtct agattttaac ttcccatttt cattaacata attttttatt
    11401 tcatctccat cattctccca gttaaccacg tattcattgt ttccatacca ttttctaaaa
    11461 cgaccacctt tagttgtagg aaaccatttt tttctagact ttttagcgtc ctctaatgtt
    11521 ttagcatcaa actttatttt gttaaattct acttcaaacc aataccgcaa aaatttattg
    11581 ttatttccgg ttgccaaccc ttgttttgca tctgcaacat cgttagttaa ttttccttga
    11641 gcaaaatcag tgattacgtt atcgcttgcc cagtaagcaa tcggcattcc tgggattttt
    11701 ttgaagttgt cttgattagt actataaaaa taatcgcatt caccatgctg atgaattgct
    11761 tctaacgttt taattttttg aacttccatt ccacctttaa aatctgataa ttttatatag
    11821 cttccatctt gttcatttga attttttatc gtaaacgtac aaattggaac ggttgcttct
    11881 tcaaaagctg aatactccat ttgaatcaaa ctagaaatgt ttctttcatt aattaaaaat
    11941 gagcgtaact tttcatagct agaaatgaac atccaaacaa atggcgtcat aaaggaagca
    12001 tatccattat caatcagcat ttggctattc aatttaataa acacagaaaa tagatctttt
    12061 ttgacatcag aatagttatc atttacatat ttttttaatt tcttatccat tttattaaga
    12121 taaggcggat tagtgactac cgctacatat ttatcaacta gcatttcagc cacgtcaaga
    12181 atgtgcaagt atttttcttg tatttccttc atcccataaa gaatatccat attcgtaaat
    12241 tcgttgaatt ttgatatata ctctcttact tttcctaagt attctttagt tattgcctga
    12301 ttatttttat cgactaactt aatgattgat ccaagttctt tcccattttc aaattgatca
    12361 aaaattaact ctttgtattc attccagcgt tcactatact tatcattgtt caattcacta
    12421 atacgctctt gatactcgtc tgtcacttta ggagaatcaa taaactctct taaattcggc
    12481 gatagacttt ttttcaaaaa aagacgatcg tattgacgcc ctttcatcat aattgcaaaa
    12541 taagacaatt gatacgctct tttatcgatt tcaagaccat ataaattgta ttcaacgatt
    12601 gcacgtgcag catcacgctc agaatatcct aacgtaagat aaatttccat caatacatcg
    12661 aaggcgtaaa ctaaaatatg cccagacccc attgcgttat cgataatttt tagttcttct
    12721 ggttttaaat tacttacaga tgttgcttgt tcaaaagcat atttatctgg aattaagtat
    12781 ttcaacttat ttcttaattc actattggga taatgatcta accaatactt tcctaaacta
    12841 ttgtcaacca tataacgaac gacccaatct gtggtaaata gttgagttgc tgccggaata
    12901 tcttgttttt tgacaatccc tttataaata ttcaccactt gattatgtgg ctcactatta
    12961 tagtactgat acaaccaccc aataatctct acctgtccac cagcttgaac atagaaatca
    13021 tcctctggaa tacttaaaag atcagcgatt acaccatctt ttttaatata agaagctgta
    13081 aacaaaagtt ctgtataatc ttctacttct tcaaatagct caggtaagta atcattcaat
    13141 tgattacaga tttttataaa aatcaattga tacagcttgt ccattgcttc tgctgaatta
    13201 tctgttttcc aagttctgac ttgttgctgt tcattactag ataattcttc aaagatatcg
    13261 gcatcataaa tatctgtaat aatatctggc tcaattttcc cacttatttc actagataat
    13321 acacgatacg tgtcaggtaa ataatcattt acctccataa accgaatggc aatcaatcgg
    13381 ttaaaccacg tataagcaac ctcttcgatt aactcttgaa aagcttctga ataagaagtg
    13441 acagaagcac gttgttttag ttctgcaatc aatttgtttc tttgcgctat ttcttttcct
    13501 tgaattctta ttcctgattt ttcgatttct tgtaaattgc tatcaatttt cttcacatct
    13561 tcaatttttg attcatcaat tcctaaacgt tttgctttag tcattacttc atcaattaat
    13621 tttctacggg cattgaccgc gaaattcttt atcgctttct tatccatttt tctttccctt
    13681 ctatatgtaa cctttgaaaa gacaggcacc tttttatgcc tgtcttttca ctagaattga
    13741 atcgtgattt ctgtattttc ttgaagttgt tccttcagtt tttgattaat ttcagctgtt
    13801 aactcatcaa tatctgctgc tgacttaata gttttaacat gaacgttaag tgttctcatt
    13861 ggtactgttt tctgttgaat agtaggtttt actatcgcta tttcttcagc ttttgctgca
    13921 gagtctattt catctttttc agtttcacga atcaagtttg ctgcttcaat ttgtttacgt
    13981 gcttgttctt cttgacgtct gcggttttct tcattatcga aacgttgatt aaagatcaaa
    14041 tactgttctg ctacgtttaa tgataagaaa acaagatcat ctatagtatt cgcttgttca
    14101 atttttgctt tcagttcttg aaattcttgg tcaactattg gtacaaattg atccttaaaa
    14161 tcttttcctt ctaataattt aaatgttgct tgtttttctt ccgttaattt atttaatatt
    14221 tcctgacttt ccaactctaa cagttgatta tattgttcaa taaattcttg aatcaactca
    14281 tttagtttca tcatatctga ataaggatca gcctttttca aaatggtttt catctcatga
    14341 atcaattgat caatttcttg gctagaagtt tgacgccgac ttttttcgat taatctaatt
    14401 ttatcttgtg cattctccca gatttttttc tgagtactat tttgcttgtg gaaattttta
    14461 attttatcat atgtttcagc ccagtcgaat aattcatcgc gatatttttt gattgtataa
    14521 aaaaagtctt caacatctga cgtttgaaca attacttcaa gatatttttt tatttgctca
    14581 tattcataag cactagggta ggtataagtg ttttgtaaat caaaataaga gcgtaaattg
    14641 tctaattgac cttttaaata tttcttgaaa ttttccattg tatgttcatt gttattttca
    14701 tcaacaatat tacgactttg aaacagttct ctaccgattt cgtcaaccaa tttttttgct
    14761 tttggcgaaa tcaccgcgct tggttcaatt tttatctttt cactttctcg tcgtttttga
    14821 ataatcatta caatactatt tggatcatcc atccgtgaaa tttgtacatt gttataaagt
    14881 aaatcaattt taccagacat gaaaagttta gcgatgcacc attccacatc aagttctgtg
    14941 aatccatacg gttgttttgt ttcaaaacga tcacgaattg ttttcatcgt aatcgttagg
    15001 tgatttctct tttgatcatt gataaaattg aaaatttcct gaactgcttg ctcatttgat
    15061 ggatcttcaa taatttcatt tttgctttct ttcactaaat ataagatatc ttctttttct
    15121 ttaggcgcat caatttggtt taatttatgg tatacttctt cagccatttt ctgaagtgct
    15181 gattggaatt ttgctttaaa gtcggagcct gtcagttgaa tttctgcacc gttatagaaa
    15241 aattttgcag atttcaacag ttcatttaat tgatcgttga tacttgcact tagttcctgt
    15301 aattcaccag atttttccga acgaatcgcg tcaaagttct ttactacact cgaataagtc
    15361 gttgttaaat aaagattcat tctagcatac attgctatat catctttaaa ttctttatct
    15421 gatggcaatt ctacgaataa taagttacgg gaactcttaa gcgcaagttc tgcctcttga
    15481 cctgctaaat cagagtatgg tgaaataaaa ttaactgtca tttcagcatt tccagcttgc
    15541 ttgtatggac gttgatctaa cacttgtgca taagtaaacg tataccggtt tttaaattta
    15601 tttccacctg acatgcctaa tttttcatag gcaaaactat ttcggtctag aacgccgtca
    15661 aaaattcgtt tagatagttc tacgatcaaa tcactatctg taatattttg atttttaatc
    15721 tcacgagtaa tttcctgttc ttcatcagtt tggaaaatat aactatctaa gttcttccgc
    15781 acaaagtttt ccttaattaa aaggtccaat gcttcttcta ctcgtttttt agtttccagg
    15841 cggtcttcgt tgatatttct tatcatcaag ctcgtaatat tttcaacatt tggtttgatg
    15901 gcttgatcaa catatttaat catgaataaa attcgtaaga catttacgtt aaagttatct
    15961 atatcagttg gtctttgaat atgagaatta tttgtagctt gtgtaatcac tcggctaatt
    16021 gctgtatcaa tccaacgttc taacgagtaa taaaactgat ctaaagaaac caaactggtt
    16081 gtatcatttt ccttatttcg ttcagcggct tctttaaaca ttgctagtaa tgaacgttca
    16141 ccagacgata agtttgatcc ttgatagctg tgattacgaa tctcggttag aatttgcccc
    16201 aataaattga attgataggg aataaatgga taaacttctg cgtaattttc agcatcttgg
    16261 aagagcttta attcaggact atcaacaaag agtaatttat ttttcaattc tgtttgaaca
    16321 gtttcaaatt gtaaggctaa agaatcattg gcaaactctt ttttcttcaa aatacgttct
    16381 ttaattactt catcggcatt tgctgaagat aaactcacag ttgtggcaaa acgatccttg
    16441 atcttagaaa aatcattatc agaattgtta tcaaagtttg ttacgtgatc aatcgcttcc
    16501 tgacttgtca ctaataccca agccttacct tcacaaattc gacccaaatc ttccacaacg
    16561 gtttgtaact gcaaagtcaa ctcacgatta ccgccgatat attgtccgac ctcatcgact
    16621 agaaaggcaa tatgatggtc tgcttctttg gaatcaagat agcttttaac tagatttgca
    16681 aagtcatcaa tgcttattaa ataatttcct gatgcttttt ctaaaaaatt atcaatagcg
    16741 gcttcactca taaaatcgat tttaagtgcc gcttctttga tatcatcttg gatccaatca
    16801 aactcatggc gctcttcttg ccattttgcc ccattaatct cttcaaaagc agctttgaat
    16861 gcatcaaact tttcttcttt aattaaccga cgttcaaaat ctgctagatg tggaatcgtg
    16921 cttaatcctt gcatttcgtt aaataccttt aaaaaaacat tcacgattcc gtttttgtct
    16981 tttttaccac ctgcatcact ttttgaatca atattaaaca gaataacatc tgtatttatt
    17041 tgtgagcacc gttccatcgt tgccaaagtc agtggatctt tgattttccc atcttctttg
    17101 aagtaatcaa tggctttctt gtcatttatt tctcgatttg cgagcaagta agaaataatt
    17161 tttaagaaat gagatttacc tgaaccaaag aacccctgaa tccaaacacc cattttatct
    17221 gttggtgtat tgatactccg ttggtaggca tcaaaaattg ttttaaagtg tttttgtagt
    17281 tctttcgtaa ccacgtattc ttctaattct tgttgaacat tttcatcctt tgcttgaccg
    17341 accttaataa ctccttgaat atttcggtca attggttttg caaaaatatc tttaattagc
    17401 atttaatctc ctaccttcca tcccaactaa attctaaatg ctcgataata attatcatct
    17461 ttattggtat taagaattgt caaacttaat tcatcatatt cccctggata catcatcaca
    17521 actggtacgc gatcaaatac ctgatgcatt ttatttaaaa tagagtgcgc tctaattaat
    17581 ggatataatt cgccaattcc tgtcaggaac acaactgatt tttctggtgt atgggcatcc
    17641 acatattcaa aaaatacatt ttcttcctca ggtaaatcca ttttcaataa acgaccaatg
    17701 ctttccacta atttttgtaa accacgtcgt tcttccatct taatcgcttt ttcagtgaaa
    17761 ccgcgttttt ctagatactc taacataatc acatagagat tataaacttt cacttcataa
    17821 ttatcaccaa cagatgttcc agatatatca ttaatataat ctcttacaat taactcgtct
    17881 ttcggatcgt agtcaaagat ataatagcct acttcattac ctaaaccacg attcaataaa
    17941 aaatcttctt tttgaattac ttcattcaac ttttccaaat gctcttgaat cgataatgac
    18001 attataaaat tcctcccagt gctagtacaa tatctttacg attcttgtca actaaccatt
    18061 gaatcaatcg tttatctaat aatatcggag tcattcgtcc atattctttt tcccctctga
    18121 ctaatcctgt ttcaattaga ttcgtttgat aagcggcaat aagccgttta atcgtataat
    18181 ctttccattt tgaaatatct tctgaacttc tccgcttctc tgtaaaaaaa gaacggtaat
    18241 caactggtgt taattgcaaa tttcctttac gtagattttc agcatatacc tcagtcataa
    18301 attcattaaa aagatcatca atcataagga tggaaatcaa attcactaac ttttgattat
    18361 ctatatttaa ctgattgaaa agttgcaatc cagcttcatc cagttgacta actctccttt
    18421 tcacgttctg atagatttct ttttgacggt tactgctcac tgcaaaaaaa atattttcat
    18481 gttcattttt ctctttaatt tctatatctg tatatccttc tagtcgtaat tgaatatata
    18541 acttgaactc tgaaaaccaa aagcgtttag aaactagacc agcagaatat tttttattca
    18601 atagattgcc tcctaatcat ctataattca ataagtaaac aagtgttttt tgctcatcgt
    18661 tagcattcat tttttagtat ctactataca atacatattt attata
//
LOCUS       DAYYBG010000052        18700 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000052 DAYYBG010000000
VERSION     DAYYBG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.053"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..1448
                     /locus_tag="ACHAUN_001722"
     CDS             21..1448
                     /locus_tag="ACHAUN_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8460420.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="ACHAUN_001723"
     CDS             complement(1540..1905)
                     /locus_tag="ACHAUN_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8460421.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="ACHAUN_001724"
     CDS             complement(1902..2267)
                     /locus_tag="ACHAUN_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8460422.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="ACHAUN_001725"
     CDS             complement(2459..2707)
                     /locus_tag="ACHAUN_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460423.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="ACHAUN_001726"
     CDS             2706..2834
                     /locus_tag="ACHAUN_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8460424.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="ACHAUN_001727"
     CDS             2891..4585
                     /locus_tag="ACHAUN_001727"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8460425.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4652..4858
                     /locus_tag="ACHAUN_001728"
     CDS             4652..4858
                     /locus_tag="ACHAUN_001728"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460426.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5236..6369
                     /locus_tag="ACHAUN_001729"
     CDS             5236..6369
                     /locus_tag="ACHAUN_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8460427.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6400..6720
                     /locus_tag="ACHAUN_001730"
     CDS             6400..6720
                     /locus_tag="ACHAUN_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8460428.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6749..7213)
                     /locus_tag="ACHAUN_001731"
     CDS             complement(6749..7213)
                     /locus_tag="ACHAUN_001731"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8460429.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7345..8598)
                     /locus_tag="ACHAUN_001732"
     CDS             complement(7345..8598)
                     /locus_tag="ACHAUN_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8460430.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8627..8944)
                     /locus_tag="ACHAUN_001733"
     CDS             complement(8627..8944)
                     /locus_tag="ACHAUN_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8460431.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8970..9290)
                     /locus_tag="ACHAUN_001734"
     CDS             complement(8970..9290)
                     /locus_tag="ACHAUN_001734"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8460432.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9754..11169)
                     /locus_tag="ACHAUN_001735"
     CDS             complement(9754..11169)
                     /locus_tag="ACHAUN_001735"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8460433.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11186..12502)
                     /locus_tag="ACHAUN_001736"
     CDS             complement(11186..12502)
                     /locus_tag="ACHAUN_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8460434.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12652..13350)
                     /locus_tag="ACHAUN_001737"
     CDS             complement(12652..13350)
                     /locus_tag="ACHAUN_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8460435.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13639..14628)
                     /locus_tag="ACHAUN_001738"
     CDS             complement(13639..14628)
                     /locus_tag="ACHAUN_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8460436.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="ACHAUN_001739"
     CDS             complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="ACHAUN_001739"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8460437.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15716..17350)
                     /locus_tag="ACHAUN_001740"
     CDS             complement(15716..17350)
                     /locus_tag="ACHAUN_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8460438.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17337..>18700)
                     /locus_tag="ACHAUN_001741"
     CDS             complement(17337..>18700)
                     /locus_tag="ACHAUN_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460439.1"
                     /translation="YYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTI
                     IPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQI
                     KNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSII
                     QALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQ
                     KTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAK
                     ENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESW
                     EKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVN
                     AGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKN
                     ENK"
BASE COUNT         5902 a         3692 c         3132 g         5974 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
     4741 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
     4801 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
     4861 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
     4921 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
     4981 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
     5041 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
     5101 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
     5161 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
     5221 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
     5281 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
     5341 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
     5401 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
     5461 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
     5521 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
     5581 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
     5641 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
     5701 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
     5761 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
     5821 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
     5881 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
     5941 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
     6001 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
     6061 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
     6121 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
     6181 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
     6241 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
     6301 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
     6361 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
     6421 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
     6481 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
     6541 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
     6601 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
     6661 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
     6721 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
     6781 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
     6841 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
     6901 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
     6961 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
     7021 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
     7081 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
     7141 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
     7201 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
     7261 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
     7321 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
     7381 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
     7441 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
     7501 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
     7561 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
     7621 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
     7681 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
     7741 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
     7801 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
     7861 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
     7921 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
     7981 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
     8041 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
     8101 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
     8161 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
     8221 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
     8281 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
     8341 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
     8401 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
     8461 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
     8521 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
     8581 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
     8641 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
     8701 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
     8761 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
     8821 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
     8881 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
     8941 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
     9001 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
     9061 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
     9121 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
     9181 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
     9241 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
     9301 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
     9361 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
     9421 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
     9481 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
     9541 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
     9601 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
     9661 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
     9721 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
     9781 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
     9841 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
     9901 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
     9961 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    10021 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    10081 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    10141 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    10201 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    10261 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    10321 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    10381 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    10441 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    10501 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    10561 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    10621 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    10681 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    10741 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    10801 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    10861 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    10921 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    10981 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    11041 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    11101 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    11161 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    11221 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    11281 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    11341 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    11401 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    11461 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    11521 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    11581 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    11641 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    11701 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    11761 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    11821 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    11881 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    11941 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    12001 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    12061 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    12121 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    12181 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    12241 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    12301 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    12361 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    12421 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    12481 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    12541 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    12601 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    12661 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    12721 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    12781 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    12841 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    12901 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    12961 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    13021 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    13081 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    13141 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    13201 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    13261 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    13321 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    13381 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    13441 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    13501 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    13561 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    13621 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    13681 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    13741 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    13801 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    13861 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    13921 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    13981 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    14041 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    14101 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    14161 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    14221 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    14281 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    14341 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    14401 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    14461 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    14521 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    14581 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    14641 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    14701 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    14761 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    14821 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    14881 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    14941 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    15001 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    15061 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    15121 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    15181 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    15241 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    15301 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    15361 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    15421 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    15481 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    15541 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    15601 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat
    15661 ccttatcgac agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc
    15721 ttttatggaa aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag
    15781 aaataatagt cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc
    15841 cgcttatttg taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt
    15901 ctacattcgt tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag
    15961 ttactctata tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc
    16021 caatctttgc ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat
    16081 aatcatataa aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta
    16141 caacaatatg tcccggctga gaaattattt tttgattgaa atttttttta ctagaaacag
    16201 ttaaagtgat taataacaaa acagtcataa ataaataaaa tagttctctt gtcttattgt
    16261 atcttctatt tttgaaaaat atcatcgtta atatcagaga agagaatatt atttgcaagc
    16321 ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt attttgcaga agataccatg
    16381 gcaccaaact agttgtagct agccaagtca aacacgtacc ataataaatc tttttttcta
    16441 tgtttgttaa ttttttaaat aaaatcaggg gaatacataa agataacaga ccaactagtc
    16501 ccattgagta agtagttaag tcgttgttca tagcttcagt cagactatcg aacacattag
    16561 atgcatgagc atataaattt gtcacgctag gtttgttgat tttttggtag agcatttgtt
    16621 caaacatcgg ataccaaaaa gcactcgtca gaaccaatgt cattcctgcc gcactaaata
    16681 attttttaaa tatagcaata aaatctgcat tcttgaaacc tttagaaacc aaacgaataa
    16741 aaataaaaat gataattaat gctgtactca ttaatgttga tagtacatgg ctgtacacta
    16801 gcaaactcat actcaatgct agctttacag acggatactt ttcttctttt ccagagatga
    16861 tttgataagc ataaaataat acagggataa caagtgttat tgcgatagct tcggcaattg
    16921 cggaacgata gtagatgtct actgtccgat aagcagagaa aatatagaga caactaaata
    16981 aaaatgccgc ttttttttgt tttaaaaacc gctcgccata atgataagct acgagtgttg
    17041 tacacaagtt caataaccaa acgtaaataa tatagcttac aatcaaattt tttgtcatcc
    17101 aatataacat tacagctgga aaaaaagtca gaaagggata aaaataattt acaccatatc
    17161 cagtataatt gaatgtctca taattaatcg gactggtaaa aatagtcgat aaccccttga
    17221 ttcgagataa gtgaaaagaa agatctccag tggtataaat actctgtgta tttttgaaaa
    17281 agatgaaaga atatatacta ctaactataa ttaatccact caatattact acttttttat
    17341 ttattttcat tcttcttgtt tatctccttt ttagtcacat atcgagaatc tttcaactta
    17401 ttaggtgcaa atcgtttaaa acttgaatac accactaaac aataagtcag aatactgatc
    17461 cataaagcca tgagagcagt tggagtatat tttgttgtaa tttctatctt attttctcca
    17521 gcatttacat ccacattcac taagccatgt tttttataag agtcatttac gactttaccg
    17581 tttactttta cctgtgtacc taaataagca tatactggta ctgtcacttt ttgctttttg
    17641 ttgcttgcca ctgtgtaagt aatgttatta tctgcagctt tatacttccc tttttcccac
    17701 gattctccta cttttacttc atgtttttct aagtgaggcc tttcttttat cgcttctgtt
    17761 ggggcgtaat cgtataatcc tcccatatct gatgttgtat aacgtgggag tgtttcttca
    17821 ttgataacaa tatgaccgtg ttttgattgg ataacttttg cgtaattttc ttttgctgct
    17881 atagacgtga cgagtagaaa caccacagta gcgccaatga ttaaccatga atatttttta
    17941 ttcatagtct ttttcataaa aacaagcgtt atgatcattg aagaaaaaaa gatttggata
    18001 cctaagatac gccaaggaaa ctggagtaag cttatcggtg ttttttgaaa caaaaaccat
    18061 ggaaataaat tagttacgca tacccatgta gaaactacgc caacatagat gattttttct
    18121 tttttctcca tttctttcag tatgaataaa ggaaaaatca acgcaaccaa tcctaataac
    18181 cccatagtgt atgttgtaat atcgttattt aacgcttgaa tgatactatc tgaaatagaa
    18241 agtgcttcta gctgcaaatc tgttttgaag ggactgtgta tttcttgata attcatctgc
    18301 tgcaacatag gataccaaaa atagctagtt aatgcacaag tcaaaaaaac agcctttact
    18361 aaattcacga ccacaaattt tatatcgttt tttatttgct tgcttaaaaa caaacgaact
    18421 ataatcatac aagcaatcaa tgcactacac aatagtgtag ttaagacatg agtatacaca
    18481 agtagactta tactgacaga taaagcgatg actttttttt ctctattgaa tatcaatcca
    18541 taagcataat ataaaacagc tgggataatc gtaagagcga ttgcttctgc aattgcggat
    18601 cgataataga tatcaactgt tcgataagtt gcaaaagtat ataaacaact aaataaaaaa
    18661 gcagcatcta tctttttaaa aaacttttct ccataataaa
//
LOCUS       DAYYBG010000053        18530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000053 DAYYBG010000000
VERSION     DAYYBG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.054"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..772
                     /locus_tag="ACHAUN_001742"
     CDS             125..772
                     /locus_tag="ACHAUN_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8460440.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    901..1117
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1165..3810
                     /locus_tag="ACHAUN_001743"
     CDS             1165..3810
                     /locus_tag="ACHAUN_001743"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8460441.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4120..5433
                     /locus_tag="ACHAUN_001744"
     CDS             4120..5433
                     /locus_tag="ACHAUN_001744"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8460442.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5423..6076
                     /locus_tag="ACHAUN_001745"
     CDS             5423..6076
                     /locus_tag="ACHAUN_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8460443.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6131..6823
                     /gene="radC"
                     /locus_tag="ACHAUN_001746"
     CDS             6131..6823
                     /gene="radC"
                     /locus_tag="ACHAUN_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8460444.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6973..7173
                     /locus_tag="ACHAUN_001747"
     CDS             6973..7173
                     /locus_tag="ACHAUN_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8460445.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7427..9106)
                     /locus_tag="ACHAUN_001748"
     CDS             complement(7427..9106)
                     /locus_tag="ACHAUN_001748"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8460446.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9108..9320)
                     /locus_tag="ACHAUN_001749"
     CDS             complement(9108..9320)
                     /locus_tag="ACHAUN_001749"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8460447.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9713..11443
                     /gene="efrA"
                     /locus_tag="ACHAUN_001750"
     CDS             9713..11443
                     /gene="efrA"
                     /locus_tag="ACHAUN_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8460448.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11440..13200
                     /gene="efrB"
                     /locus_tag="ACHAUN_001751"
     CDS             11440..13200
                     /gene="efrB"
                     /locus_tag="ACHAUN_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8460449.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13295..13492
                     /locus_tag="ACHAUN_001752"
     CDS             13295..13492
                     /locus_tag="ACHAUN_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460450.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13645..14130
                     /locus_tag="ACHAUN_001753"
     CDS             13645..14130
                     /locus_tag="ACHAUN_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8460451.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14234..14827)
                     /gene="clpP"
                     /locus_tag="ACHAUN_001754"
     CDS             complement(14234..14827)
                     /gene="clpP"
                     /locus_tag="ACHAUN_001754"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8460452.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15007..15534
                     /locus_tag="ACHAUN_001755"
     CDS             15007..15534
                     /locus_tag="ACHAUN_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460453.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15625..16362
                     /locus_tag="ACHAUN_001756"
     CDS             15625..16362
                     /locus_tag="ACHAUN_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8460454.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16366..17283
                     /gene="miaA"
                     /locus_tag="ACHAUN_001757"
     CDS             16366..17283
                     /gene="miaA"
                     /locus_tag="ACHAUN_001757"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8460455.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17285..18514
                     /gene="hflX"
                     /locus_tag="ACHAUN_001758"
     CDS             17285..18514
                     /gene="hflX"
                     /locus_tag="ACHAUN_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8460456.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5991 a         3204 c         4027 g         5308 t
ORIGIN      
        1 agatgcaagc ttttgaacac cgttttattt gtctcacacc cttttccttc ctacttgtaa
       61 ccttgctccc gtgttagaaa catgttacac taaatatgtg aaattattaa caaggagcgt
      121 cgttatgcga gaaaaaacag atacactgcc aaaagcaact gcgaaaagat tacctttata
      181 tttacgctac ttgaagatgc tggatgactc aggtataagt agaatcaaat caaatgagtt
      241 cagcgaaatc acccaaattc cttctgcaac gatccgcaga gacttctcgc aattaggcga
      301 gctaggcaga agcggatatg gctatgatgt cccttttctt atcgatgtat tcaataatat
      361 ccttaatact aaagaagaaa aacgaatcgc attagtagga tatggaaatc ttggcaaagc
      421 attgaaacac aacaatttta gaagaaatga aaatctaaat atcgtgtgcg tctttgataa
      481 tgatccagca ctgatcaatc gagtaattga tggagaaatg atctatccga ttgatcgttt
      541 cgcggagata gccaaagcaa aaaatgtgac agtagcaatc tctacggtac caagtaaata
      601 ttcacaatct gctattgatg aaattgtcaa aggaaatgta accgctatac ttaattttgc
      661 gcctgacaga gtaactgtcc cagcttatgt caatgttcaa tacattgatt tgacaaccga
      721 gttgcagacg cttatttact ttgatgagaa ttactcagaa gttttttctt aggaaaattt
      781 gagtaggctt attgatcttg cgagaactcg tcgtaattct ttcaaaaatt ttttagtact
      841 aaatgaagtg gttttttcac gaccacttgt tgactaaaaa aaaaaaccat tgtagaatga
      901 agcataacgc tgaacaagag gagtaactgg cacattcatt cttagagaga gaactatctg
      961 ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg agtttctgtc ttgaatggag
     1021 tagagataga cgagtcgtga tcgttaactc acttgagagg caataatgat tgattattgc
     1081 aatttaggtg gtaccgcgta aatcatacgt cctagaatac gaaagtattt taggacgttt
     1141 tttagtttaa aggaggaaaa cagcatgacg gaagaaaata acctatcaac aaaatataat
     1201 ccgcaagaag ttgaagctgg acgttatcag cattggttag aacaagattt gtttaaaccg
     1261 agcggagata aaaaagcaaa accttattca atcgtaatcc cgcccccaaa tgttactgga
     1321 aaacttcatt tagggcatgc ttgggatacg accttgcaag atatgctgat tcgtcaaaag
     1381 agaatgcaag gatatgatac tttatggctg cctggtatgg accatgcagg aatcgcgaca
     1441 caagcaaaag tagaagcgaa gcttagagag caagggattt ctcgttatga tctaggtcgt
     1501 gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt atgcgagcca catccgcgaa
     1561 cagtgggcta aaatgggctt atctttagat tacagtaggg aacgttttac attagatgat
     1621 ggattatctg aagcagtacg taaagttttt gttaccttat acgaaaaagg tttgatttat
     1681 cgtggtgaat atatcatcaa ctgggatcct caagcacgaa cagctctatc tgatatcgaa
     1741 gttattcata aagatatcga aggcgccttt tatcatatga gctatgaact agcagatgga
     1801 tcaggcgtag tagaaattgc gacaacccgt ccagaaacga tgttgggaga tacggcgatt
     1861 gctgttcatc cagaagacga gcgttatcaa gcgctcatcg gcaaaaaagt gattctacct
     1921 ttagtcaata aacaaatccc aatcattgca gatgaatatg tagatatgga attcgggaca
     1981 ggtgtagtaa aaatcactcc agcacatgat ccaaacgact ttgaggtagg taatcgtcat
     2041 gatcttccac gcgtaaatgt tatggatgat gatggtacga tgaatgaact agcaggaaaa
     2101 tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc
     2161 ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg gtcattccga gcggacaggt
     2221 gtcgttgttg aacctcggct gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa
     2281 aaagccattg ataaccaaga aacagatcaa gcagtggaat tcttccctcc aagattcaat
     2341 caaacgtttt tacgttggat ggaaaatgtc catgattggg taatctcacg acaattatgg
     2401 tggggacatc aaattcctgc atggtatcac aaagaaacag gtgaaatgta tgtagggatg
     2461 gaagcaccag cggatagtga aaactgggta caagatcctg atgttttaga cacttggttt
     2521 agttcagctt tgtggccttt ctcaacaatg ggctggccag atacggaaag cgaagattat
     2581 aagcgatact tccctactag cactttagta accggttatg acatcatttt cttctgggta
     2641 agcagaatga ttttccaaag cttggaattt actggcgaac gtccatttga gaatgtcttg
     2701 attcatggat tgattcgtga cgaacaaggg cggaagatga gtaaatcatt aggtaatgga
     2761 atcgatccga tggaagtaat cgaaaagtat ggagcagatg ctctccgctg gttcttatcg
     2821 aatggttctg caccaggaca agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg
     2881 aactttatca acaaaatctg gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg
     2941 accgtagaag agatcgattt atctggagaa aaatcagtgg ctgaccggtg gatccttact
     3001 cgtttgaatg aaacgatcga acgagtaact gagctatttg atcgttttga atttggtgaa
     3061 gcaggtcgac aactgtataa ctttatctgg gatgattttt gtgactggta catcgaaatg
     3121 agtaaagaag tactttatgg agatgatgct gtttcaaaac aaaccacccg tagcatctta
     3181 gtccatacac ttgatcagat cctacgttta ttgcatccaa tcatgccatt cgttacagaa
     3241 gaaatatggg aacatattcc tcatcaggga aattctctag tagtggcaga atatcctgtt
     3301 gtacgtccag agtttgatga tgaaacagct tctaaaggga tggaagtatt gaaagaattg
     3361 attcgagccg ttcgaaatat tcgttcagaa gtgaatacac cattgtctaa accaattacg
     3421 ttgctaatta aaacaaacga tgctaaaatc gatcaatttt tagttgaaaa tacaagctat
     3481 atcgaacgct tctgtaatcc cgaagagttg acgatatcaa gtgagatcac tgcgccagat
     3541 ctagcgatgt cagctgttct gacaggtgca gaaatcttct tgccgttagc aggattgatc
     3601 aatattgaag aagaaatcaa acgattagaa aaagaactgg aaaagtggac aagcgaagta
     3661 aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta
     3721 gtagaggcag agcgtgcaaa agaaaaagat tatttagaga agcaaacggc tgtcaaagaa
     3781 cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa agagagtgtg aaatcagcta
     3841 gctattacta aaagctagct gattgaacat cgttacagga aagagtttga gcctcactct
     3901 tagcttcgat tcataaggag atgtgacata aattttgtcg catctctttt ttagtctact
     3961 cttattatgt aggagaagga gggaggtgga tcatgtatca agagtcaata aagtagttga
     4021 taaaatcgtt cctctctctg tttcttttct ctttgtcata cttttatttt tcttatgtgt
     4081 tctatttgtt ataataaaag agaaatgggg gaattgttga tgacgattga agaagcaata
     4141 gaatggatcc atagtcgatt accatttggt tcacgtcctg gccttgaccg agtcgaagct
     4201 ttattagaag tggtagggca tccagagaga aaagtaaaaa cgatccatat cgctggaaca
     4261 aacggaaaag gttctactgt agcttatttg agaagcctat tagaagaggc tgggctgaca
     4321 gtaggtacat ttacttcacc ttatatcgaa acgttcaatg aacggatcgc aatcaatggc
     4381 aaaagcattc cagacaatga tttgatccag cttgtaaaaa aatatcagcc actgatcaaa
     4441 caacttgatc aatttgaagc agtagctggt attactgaat tcgagacatt aacagcaatg
     4501 gcatttgatt attttttgat gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc
     4561 ggcttattgg attctaccaa tgtagctgta ccaatgctga cgggaataac aacgattgga
     4621 cttgatcata ccgaaatact tggagataca attgaagaaa tcgctgcaca aaaagctgga
     4681 atcatcaaac aaggcgttcc agtagtaaca ggaaatattg ccccagaagc ttttactgtc
     4741 atccaacaaa cagctaaagc aaaaaatgca ccgctttatg tatacgggga aaattataaa
     4801 ataaactatc tgcatcctga tgagatgtgg ggagaagtat ttgaattttt taatgaaata
     4861 ggaaagatcc ctcaattaac cactccgctt ttagggcaac atcaagctga aaatgcagga
     4921 gtagccatcg aattgtattt tgtttactgt caattggaaa agctgccttt ccgagaaaaa
     4981 gatgttttga agggattgaa gaaatctcga tggccaggaa gaatggagcg tttgagtaac
     5041 gagccattag ttgttttaga tggtgcccat aatacgcatg caatgaaccg gcttgttgag
     5101 aatttgaaaa aagaattcaa aggatatcaa atcaacattt tgttttccgc cttacgaact
     5161 aaaaatgtta gagaaatgct agaacgattg cagcaagtac caaatgtcca tatttatttg
     5221 acgacctttg aacatccaaa agccatcgaa ttaagtgaag tatcagattt agcaagtgag
     5281 agagtatcaa tcgtttcatt gtggcaattc ggattagcag atatattgga aaagatgaca
     5341 aatgaagacg tattagttgt gaccggttca ctttatttca tttcagaagt tcgccattta
     5401 ttaatacaat taggaggaga tcatgaagta taatgcagtg atttttgaca tggatggttt
     5461 gctgttcgac acagagttag tgtattacga agccacacaa gtagtagcag acaagatggg
     5521 ttttccttat gataaagacc tgtatttaaa attcttaggt gtttcagatg aagaagtatg
     5581 ggcgaattat catatgattt tctcggaatt tggcaaaaca agtgtacaaa gattcataga
     5641 tgattcatac caggaaacgc ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg
     5701 tgtgttggaa ctcctctctt ttttagaaga acatcagatt cccaaagttg tcgcttccag
     5761 taaccaacga gcagtcattg aacttttatt gaagaagaac aatctgtttg aacgatttga
     5821 gacgattgtt tctgcagaaa atgttcaacg agcaaaacca gacccagaaa ttttcttgtt
     5881 agctaatcag aagctaggta cagcgaagaa agaaacattg attttagaag attcacaaaa
     5941 tggtatttta gcagcttctg cggctgagat cccggtagtc atggtaccag atctgcttcc
     6001 accatcccaa gaattgatca aaaaaacagc tggggttgtt tcttctttac atgaaatccc
     6061 tgcattgtta aaataaaaat ccatctcttt tcgtatctta ttttagataa gatagaaagg
     6121 agatgttttt ttgggaaaac gattgatcaa agaagtacct actagctctt taccaaggga
     6181 acgtatggaa atttatggcg cggaagcttt atcggatcag gagcttttgg caatcttact
     6241 gcgtaccggt caacaccctt atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt
     6301 tggaggactg gcctctttac gacaggctac tttgcatgag ttagaagaga ttcgagggat
     6361 aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac
     6421 ggatcatgaa agagcggctc caccggttcg taccagctac gaattggccc agcaattgat
     6481 tctggaaatg aaagatcaca aacaagaaca tttggtttgt atttatttgg acacgaagaa
     6541 tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag tcgctcatcc
     6601 tagagaaatt ttccattatg ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa
     6661 tcatccttct ggtaatgtta ttccatcgca gcaggatatg aattttacca aaaggataca
     6721 aaagtgtgga gaaatgatgg ggatcactgt actggaccac ttgatcatcg gacgcaaacg
     6781 atattttagt ttgagagaag aaggaatgat ggaagagaag taagttgctt tcttgcaaaa
     6841 aaaatgaagc aagtgtacaa ttatattgta attttgtttg ggaatagaag ttgaaacatc
     6901 tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa attagtgctt ttggcacgag
     6961 gaggatatta ttatggaaaa cggaacagta aaatggttca acgctgaaaa aggatttgga
     7021 tttatctcac gtgaagatgg aagcgacgta ttcgttcact tctcagctat ccaaggtgac
     7081 ggattcaaaa cattagaaga aggtcaagca gtgactttcg acgttgaaga atcagaccgc
     7141 ggcccacaag ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa attgatggta
     7201 gacatcaatt tttacgagag aacaaagcga gtaacaaaaa tacctgccac aagagacaat
     7261 ttctcttgtg gcaggtattt ttttgtatga aaaagtaaat aaatttaaga ggctatgaaa
     7321 gagccacctt gtatcaagga ataagaacaa acaaaaatgg cttctcatgt ttttcatttg
     7381 ttttctctta tattcaggac aaggtgctga tttcataacc tcttatttat tcttcatctg
     7441 gtgtcattac cattggcaaa atcattggat gtctttctgt acgttcgaat aagaaaggct
     7501 gaagcgcgcc agtcatcgct tctttcaatt tatattctga acagtcaggt ccattcaaac
     7561 attctcttaa agcgtggaat aagattcgct gagcttcatt gatcatttct cctgattctc
     7621 gcatgtagac aaatccacga gagagaatat ctggaccagc caagatttct tttttcttga
     7681 tatccactgt cgcgacagct aatacaagac cttcttctga aagcaggtga cggtcacgta
     7741 aaacgacatt accaatatca ccgatcccgc taccatcgat atacacatct ccagccacaa
     7801 aatgcccagc aggacgcgca gaatcgctag ttaacgctaa aacatcgcca ttttccatga
     7861 tgaagcagtt ttctgcagga acacctacat cttgtgctag agaagcatgt actttcaaca
     7921 tgcggaattc tccgtggaca ggaataaaat attttggctt catcaaacga agcattaatt
     7981 tttgttcttc ttgtccaccg tgaccggaag tatggatgtt attgattttt ccgtgaatca
     8041 cttctgcacc agcttcagac agcaaattga tcaaatgatt gacacttgtt gtattaccag
     8101 gaataggtga acttgaaaag atcactgtat cgtctggctg gatttggatt tgacggtgag
     8161 taccattcgc gatgcggctc aacgcggcca tcgcttctcc ttgagaacct gtacatagga
     8221 tcatcgtttc attggcaggc aagcgattga tttcgtgtgc atctacaaat gtgccgtctg
     8281 gaactttgat atatcctaaa cgttgaccgt tgacgatggc attttccatg cttcgtccga
     8341 aaacagcgat tttacgacca gttgccacag cggcatctgc tgcttgctgc aaacggaaga
     8401 tgttagaagc aaagctggca aaaatgatac gtccttcgat ttttgaaaag atcttcaaaa
     8461 tagaatgccc aatcgttttt tctgatttag taaatgttgg aatctcagca tttgtactat
     8521 cagataaaag gcacaagact ccttcttccc cgatacgagc cattttatgc aagtttgctg
     8581 gttcgccaac aggagtgaaa tcgaatttga agtctccagt cgctacgata tttccagaag
     8641 gtgttttcac cacgatccct aatgcatctg ggatactatg ggtcgttctg aaaaagctga
     8701 cagaagtctt tctaaaacgg atgatcgtat cttcattgat ctcatggagc tctgcatctc
     8761 gaagcaagcc atgctcatct aatttgtttg tgatcaacgc taatgcgaga ggaccagcat
     8821 agataggaat atttgcttgt ttcagcaagt agggtattcc gccgatgtgg tcttcgtgcc
     8881 cgtgagtaat caccaaggct tttactttgt gtaagttttg tatgatgtag ctgtagtcgg
     8941 gaatgacata gtcaattcca agtaaatcgt cttccgggaa tttgatcccg gcatcaatca
     9001 aaatgatttc atcttggaat tgtaccccat acgtattttt cccgatttca cctaatccac
     9061 caatagcaaa gacgcctgtc tcgttatttt tgatggaaag tttcatatta aaactccact
     9121 aatgagaaat cagcattttc tttttcgtac tctagatgac ttccttctag aagttgaacg
     9181 aattcaatat tgtatggggt attttcttcc acacgttggc gagcgtctac atcgcttttt
     9241 gcctcaatgt aaagagattt tgtttcttcc cgtttagggt tgcgtacttt tgtttcttga
     9301 taataaactt tatagatcat tgttttgtct cctttatctg cgtaaagcag tgatttagct
     9361 agttgatttt tttagacaac acaaaacgag gggacaagcc acctggtttt tcatatgctt
     9421 tgactagaat actggattcg agtcaaagag acaagcctcg tttttgctta tctgttcgga
     9481 tattttttca accgtacctt ctgtataaaa aatatacaac aattaattgt tattctagca
     9541 taaaacagca aataagtata gaaatcaact agattgaaag aaaaaatcaa tgtttatcat
     9601 cccgctataa tcataatgac ttttttatga gagaagtgtt ctattaatag gctcgttgtg
     9661 ctatcatttt tagtaatgaa aatgtaacaa aagattaaaa ggagatgaga aagtgaaact
     9721 aatgtggcat tataccatgc gttataagaa attcttgttt ttcaatttca tatgcgtatt
     9781 tggttttatt ttgattgaat taggacttcc tacgatttta gcacgtatga tcgatgtagg
     9841 aatccgcaat gatgattttg attatgtgaa gcaacaaggg atatttatga tcgttattac
     9901 tgtaatcggt attattttga atatcttgtt aggttatttt ggttcgagga ttacgactaa
     9961 tatcgtagca gatattcgag atgatttgtt cgaaaagatc caaagttatt cgcatcagga
    10021 atacgaaaat ctaggcgtat cttcattgat cactcgtaca acaaacgatg cttatcagat
    10081 catgttgttc ctgcaaaata ttttacgtat cggttttatg gcaccaatga tgttcattgt
    10141 cagtttgtac atggtcatgc gtacaagtgt gacactcagt ttgtatgtag taggtgcatt
    10201 acctttacta ttattggcag tcgtagcgat tgctaaggtg tctgaaccgt tatctaaaaa
    10261 gcaacaaaaa aacttagata aaatcaacag tatcttgcgg gaaaatcttt caggattgcg
    10321 tgtgattcgt gcattcgtca atgaaaagtt tgaagaaaaa cgttttgctg tagtcaatga
    10381 gaattatgct tctagttcaa aaagcttatt ccgtttgatg gctgttgcac aaccgggttt
    10441 ttatttctta tttaatatcg taatggtatt gatcatctgg agcggggcag tgcaaatcga
    10501 tgcaggcgag cttcgtgtag gagatttgat tgcgtttatc gagtatatat tccatgcctt
    10561 gttctcattc atgttatttg ctagtgtgtt catgatgtat ccaagagcag ctgtttcagc
    10621 tggacgtatc caagaagccc tagatgccaa accttcgatt gtagaagatc caaatggtat
    10681 atctgataca aaaactaaag ggtatatcga atttcaaaat gtaacatttg cttatccagg
    10741 ccattcgcaa gagccagtga tacgaaatgt cagttttaca gcctctccag gagagaccgt
    10801 tgcttttatt ggaagtaccg gtagcggaaa atctacgttg atccaattga tccctcgctt
    10861 ttatgatgtg agtgaaggaa agatcttgat cgatggagta gacgtgagag attatcggct
    10921 aagcaagttg cgagataaga ttgggtttat tccgcaaaaa gcgctgctat ttactggaac
    10981 gattgcagat aatttgcgat acgggaaaga agatgctacg caagaagaaa tggaacgtgc
    11041 agctgagatt gctcaagctt cagaattcat ttctcgaaaa ccagatggat ttaatgaaca
    11101 tttatcagaa ggtggttcca atttttcagg tggacaaaaa caacgattgg caatagccag
    11161 agccatcata cgccgtccgg aaatctatat ttttgatgac agcttttctg cattagatta
    11221 tcaaacggat gcgaagttac gtgctcgtct gaaaaaagaa acgacagaat ctactgtatt
    11281 gatcgtagca caacgtgtgg gaacgatcat gcatgcagat aagatcatcg tattaaatga
    11341 aggagaagtc gtcggtatgg ggacgcatcg ggagttgttg gaaaaatgcc cgatttatta
    11401 tgatattgcc gcttctcaat tatcaaagga ggaattagca tgaaaaaaac cttttcttcc
    11461 ttaaaacgtt tagctcgata tatcagaccg tatcgtgaga cttttctttt agtgatccta
    11521 tttacagttc tgacagtggc tttcaacgtg gctttgccat atgttacagg acttcctact
    11581 acagaaatca gccgtaatat tgccaatggc gaatcaatca atttttctta tatattcaaa
    11641 tgtctcattt ggatcacagt tgttggaata gggtattgta tttcgcaatt actttctggt
    11701 gttttgatga caaatgtcgt tcaaagtgcc atgcatgatt tgagaagaga catagaagaa
    11761 aaaatcaatc gtcttccggt ttcatatttc gataaaaacc agcaaggaaa tattttgtct
    11821 cgtgtgacga atgatgtaga tgcagtcagc ggtgctttac aacaagcttt tattggtatc
    11881 gtaaatgcga tattgggaat tacgttggct gcagcaatga tgttttatat ccagccgatg
    11941 atggcgctga tctctatgat catgattcca acatcgatct ggatctcaaa acgagtgatc
    12001 aatgcttcgc agaaatattt tcaaagcatg cagaattctc ttggtgagct aaacggctac
    12061 gtccaagaaa atatgacagg ttttagtgta ttgaaagtat atggacgcga aaaagaaaca
    12121 ttggaaggat tcaaaaaagt taatcacagc ttgaaatact ttgggtttcg tgcagcattc
    12181 atttctggct tgatgatgcc tctagttcaa ttaactgcct atgctactta tattggtatg
    12241 gctgttctag gaagctttta tgttattact ggggcgatcg ttgtcggtca actacaagca
    12301 ttcatccaat atatttggca aatcagtcag ccaatgggga acgtgacaca attgtcttca
    12361 gttttgcaaa gtgcatcagc ggcaacgaaa cgtgtattcg agattttaga tgagccagaa
    12421 gaaaaagaaa acaaagtaga tgcttcactg ccagaaacga ttttaggaga tgtgacattc
    12481 gatcatgtcg attttgcata tgatccgaag aaaccattaa tccaagattt gaattttgaa
    12541 gtgaaagctg gtcaaaccgt agctgtggtc ggtccgaccg gtgccggcaa gacgacattg
    12601 atcaatctat tgatgcgttt ttatgacgta gacaaaggag caataaggat tgatggaatc
    12661 gacaccaaat caatgagtcg cagtgatgtg cgttcgctat ttggtatggt cttgcaagat
    12721 gcatggcttt atcaaggaac gattgcagat aatatccgct tcggaaagct tgatgcgaca
    12781 gattatgaag tagttgatgc tgcgaaaaca gcaaacgtag atcattttat ccgaacgatg
    12841 ccagatggat atgatatggc catcaattct gagggagaca atgtgtcttt aggacaaaaa
    12901 cagttgctga cgattgcccg agcagtggtc tccgatccta aaatcttgat tttagatgaa
    12961 gcaacaagtt cagtagacac ccgtttagaa gcgttgattc aaaaagcaat ggatcgcgtg
    13021 atggagggac ggacaagttt cgtcatcgct caccgtttat cgacaatccg tgatgcagat
    13081 ctgattctag tgatggatca aggtcagatc attgaaaaag gaacgcatga gagtttattg
    13141 gcacaaggtg gattttatga aaaactgtat aacagccaat ttgctgagga agcagaataa
    13201 gtcatataag aataaaacag tttaaaactt attttttgtt ctctattctt gataatggta
    13261 aagtaaagtt atcaagaaaa gtgaggacac ggagatggaa cagtttttaa atgaaaaaat
    13321 tactgaagcc aaaagagaag ttgatcgttt aacgcacgtc tgtcagacga agccttttga
    13381 ttcagctgat ttttttgaaa atgagtttca aatcgaacgc ttggcagcac aaatagaagt
    13441 gtacgaatta attttaaaaa aagtaataga agaaaatata gatgtacaat aaaaacaggt
    13501 aaaacgtcag ttaccagata ctgtaaatag acatctggcc tgagcaggtt ttacctgttt
    13561 tttttcttat gaaataaata tatgctatga tacctttatt caataggatt agtttctaca
    13621 aaaaatgaaa ggagaaaaat aaatatggaa cggtttatag catcaaatgc cacagtgatt
    13681 ggtgatgtga cattgtcaga agatgtgacg atctggtatc aagctgtttt acgcggggac
    13741 agtaattgga taaaaatagg acaaagaacg aatatccaag atggtacaat catccatgtc
    13801 gatcatgatg cacctgtaga tattgcggag aatgtgacgg taggacatca gtgcatgcta
    13861 catggctgta caatcgaaaa aggggcgctg atcgggatgg ggaccacgat attgaatcat
    13921 gcggttattg gtgagaatag tttgattggt gctggatcat tagtaacaga aggaaaagtt
    13981 attcctccta atgtcttggc ttttggtcgc ccagctaaag tgatccgtcc tttgacaaaa
    14041 gaagaaatac aaaaaaacaa agaaaacatc cagcattatg tcgagatagg tcggatgcat
    14101 gcgcaaggaa aattcaagga gcaaagatag gcagtagcat ccataaaata aaaaaggatg
    14161 gtcagtttga gttgaccatc cttttttatt tggtttagat aagagagctt aaaaaagctt
    14221 cctttgaaac agattaattt aatgaagaac tgttttccat gacttcatcg atcaatccat
    14281 atgcttttgc ttgttcagct gtcatatagt tgtcacgatc tgtatctttt tcaatcactt
    14341 caagcggttg tccagtgcgt tcagctaaaa ttttgtttaa gcgttcgcgt gtttgcaaaa
    14401 tatggcgtgc cgcaatttcg atttcagttg cttgcccttg agcgccgcca agaggttgat
    14461 ggatcatgat ctcagcattt ggtaaagcaa atcgtttacc ttttgtacca gctgtcagca
    14521 agaagcttcc cattgaagca gccataccca taacaattgt ttggacatct gctttgacaa
    14581 agttcattgt gtcgtaaata gccatacctg ctgtaactga accaccagga gaattgatat
    14641 aaagatagat atctttttca gaatcttgag cgtctaagaa aagcaattgg gcgatgatcg
    14701 aattagcaag atcatctgtt acttggccgc ttaacatgat gatacggtct ttcaataaac
    14761 gtgagtagat atcgtaagct ctttcacctc gtgaagattg ttcaatgact gtaggaatta
    14821 aattcataat agagttcctc cttgatttcg tttagaaata gtgtaccata atttagtttc
    14881 taatacctaa aatgtagtat acatttttgg tcaaaaaagg tcaaataaaa aacttaggca
    14941 aattagtcgc ataaacccgc tttatggtat gataagcaga gaaacaaaac aaggaggaac
    15001 aacagattga tagttggtat tactggcctt atcggagtgc tgacaggtgc tgtagttgtc
    15061 tatatcggca tgatactgca aatgcgtcac ttggaaaata aagaacagcg tagacgtgaa
    15121 ttggaattga aagtcaaaga aatcgagacg ctgaatcaat taaataaaaa gatcaacgag
    15181 atcttgcaaa aacggacaac gctgatggaa gaatatgtca gctttgatgc atttgatgat
    15241 tgttatatca cgattgacga ttttgcgtat ctccaatcat tttcagcgca aaatagtttt
    15301 tatctgccaa attatatttt agacgagcta tttaagaata tctcccatcg aaaagtgatt
    15361 ttatctccag atgaaacaat gaagattggt ggatatgctt ataaaggcgg acgcgtgatt
    15421 ttagagaatt ttacggatag tctgactgaa atgatcaatg aaaaaaaagc tcagttgaag
    15481 actatgacgc gccagccgat tggtttcttc tcgaaagatg atgtttggag ctgatatttt
    15541 gaagcattgc cgttgcacat gagagcgtgg taatgctttc tttttttctt aatgatacaa
    15601 tagaactgaa cggagtgaag aaagatgtta caaatctatg cccatcgcgg aagcagcggc
    15661 acccacccag aaaatacgct tcctgcattt gcagaagctg tacgagtagg tgcagatggt
    15721 atcgaactag atgtccatct aagcaaagat ggttatttga tcgttatgca cgacgaagaa
    15781 gtggaccgga cgactaacgg taaagggctg attcgagaaa aaacattgga agaactcaaa
    15841 aaattgaatg cagggggcaa attcagcaaa gaatttccag cagctaaagt tcctgaattg
    15901 agagaagtgt taggactgct cctccaaaaa aattaccgag ggatgctaat gattgaaatt
    15961 aaaactgatc agtatgaata tcccggtatc gaagaaaaat tagccgaaac attaaaaagt
    16021 agagattggc cttttcaaca tgcatattgc agctttcatt tggattcttt ggaaaaaatc
    16081 gcattactgg aaccggagat acagctggac ttattgatga gtacttctga taaaaagatc
    16141 caactagcag cagaaacccc ttatatagaa ggagtacatc caaaaaatac ttggttgttc
    16201 gaacatccag tccaggcagc tgattttcca aaagcaattc gtgcatggac gattaacgat
    16261 gaagaagaaa tggccaaatg tcaagaactc aaaatagatg gaataataac tgattttccg
    16321 gagaaagctc ttcggtggaa acaatcaacg aaaagtaggt aaaatatgga aaaagtatta
    16381 gtgatcgtcg gtccgacagc agtcggtaaa acagcgctaa gtgtggaact cgcacaacta
    16441 tttggaggag agatcatcag tggtgattca ttgcaaatct ataaaaatct ggatatcggt
    16501 acagcaaaaa tcaaagaaga agagatgaat aatgttccgc atcatttgat tgatgtgatc
    16561 gaacctactc aacgttactc agccgttgat tttcagcatt caggtcgaca attgattacc
    16621 gagattgcga accgagggca cctacctatc atagctggtg gtacgggtct ctatatccaa
    16681 tcccttctat atgattatca gttaggagcg acagaagaag cgaatcctga gattagaaaa
    16741 aagtatgaaa cgttagctga aacaattggt tcccaagcat tatggaatca gttgaaagaa
    16801 aaagacccgg cagctgctga gaaaatccac tggaacaatc aacgcaaagt gataagagct
    16861 ctggaagttt tagagacgac aggacatagt atcacggcac ctaaagaaga gccaaagcaa
    16921 ttgtatgatt acctcatgat tggattagat acgaagcgta gtattcttta tgaacgaatc
    16981 aatcaaagag tcgatttgat gctagcagaa ggtttagtag cagaagcgcg taaggtgtat
    17041 gagcttggag aagtccaggc cagtcaaggt attggatata aagaattttt cccttatttt
    17101 gaagggaaag aaacattgga agaagcaaca gaacaagtca aactacattc gagaagatac
    17161 gcgaaacgtc agttgacgtg gtttcgtaac cgtttggatg cacagtggtt tgatcttata
    17221 agtgagccaa atcaaaaaag tcagattgag caaacaatca aaaaatggct ggaggcggaa
    17281 taaaatggaa gaaaaagtga tcatcgtagg ggtagaaacc gaagcaaacc agcgttactt
    17341 ttcagagtca atggaagagc tagttcagct gacgaatacc gcatcaggag aagtcgtatt
    17401 cacaattaca caaaagagac cacaagtgga tcggcaaacg attatcggta aagggaaatt
    17461 acaagaatta gttcaacaag cggatgcgca tgaagcggat ttgatcatct ttaatcatga
    17521 gctaactccg agacagagcc aactgattac cgatgctgtt ggtcttccgg taattgatcg
    17581 cgtgcagctt atcttagata tttttgcgat gcgtgctcgt tcgaaagaag ggaagctgca
    17641 ggttgaactt gctcagttag aatacctgct gcctcgttta gtagggcaag ggaaaacatt
    17701 atctcgtctt ggcggtggga tcggaacaag agggccaggt gaaacaaagt tagaaacaga
    17761 ccgccgacat atacggaata aaatttttgc tgttaagaaa gaattgaaag aagtagaagc
    17821 tcatcgagag cgcaatcgcc aaaaaagaaa aaatagcgag atttttcaga ttggacttat
    17881 tggctacacg aatgccggga agtcaactat cttgaatcta ttgacacaag cggatactta
    17941 ttcgaaagat caattgtttg caacgttaga tccattgact aagagatggc gttttgctga
    18001 aggatttgag attactgtaa cggataccgt cgggttcatc caagacctgc ctacgcaact
    18061 gatcgatgct ttccattcta cattagaaga aagtcaaaat atggatcttt tgctacatgt
    18121 agtcgatgct agttctccag atcgtatttt acaggagcag actgtcttaa aattgatgga
    18181 tgaactgaat atgaaagaaa tgccgatact gactgtttac aataaggcag accagatcga
    18241 cccagcgatg ttcacgccta cgctattccc aaatgtattg atttctactc agacgaaaga
    18301 aggtaagatg gcgttgatcg aagcaatcaa acgtcagctg atggaactaa tgacccctta
    18361 tacactttct gtgccaagca acgaaggaca gaaattaagc gagttgagaa gacaaacaat
    18421 ggttttggac gaagcgtaca tggaagaaac aaacgaatat gaagttaaag ggtttgcaaa
    18481 aaccaattca aaatggttaa gaaaaaacca ataaaatttt atttttttaa
//
LOCUS       DAYYBG010000054        18458 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000054 DAYYBG010000000
VERSION     DAYYBG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.055"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1502)
                     /locus_tag="ACHAUN_001759"
     CDS             complement(183..1502)
                     /locus_tag="ACHAUN_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8460457.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(1659..2045)
                     /locus_tag="ACHAUN_001760"
                     /pseudo
     CDS             complement(1659..2045)
                     /locus_tag="ACHAUN_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2230..2628)
                     /locus_tag="ACHAUN_001761"
     CDS             complement(2230..2628)
                     /locus_tag="ACHAUN_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8460458.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(2625..3398)
                     /locus_tag="ACHAUN_001762"
     CDS             complement(2625..3398)
                     /locus_tag="ACHAUN_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8460459.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(3395..4078)
                     /locus_tag="ACHAUN_001763"
     CDS             complement(3395..4078)
                     /locus_tag="ACHAUN_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8460460.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(4245..5132)
                     /locus_tag="ACHAUN_001764"
     CDS             complement(4245..5132)
                     /locus_tag="ACHAUN_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8460461.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(5129..5998)
                     /locus_tag="ACHAUN_001765"
     CDS             complement(5129..5998)
                     /locus_tag="ACHAUN_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8460462.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(6135..7421)
                     /locus_tag="ACHAUN_001766"
     CDS             complement(6135..7421)
                     /locus_tag="ACHAUN_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8460463.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            7694..8272
                     /locus_tag="ACHAUN_001767"
     CDS             7694..8272
                     /locus_tag="ACHAUN_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8460464.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            8272..9201
                     /locus_tag="ACHAUN_001768"
     CDS             8272..9201
                     /locus_tag="ACHAUN_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8460465.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            9198..10934
                     /locus_tag="ACHAUN_001769"
     CDS             9198..10934
                     /locus_tag="ACHAUN_001769"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8460466.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(10997..11833)
                     /gene="pheA"
                     /locus_tag="ACHAUN_001770"
     CDS             complement(10997..11833)
                     /gene="pheA"
                     /locus_tag="ACHAUN_001770"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8460467.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(11830..12339)
                     /locus_tag="ACHAUN_001771"
     CDS             complement(11830..12339)
                     /locus_tag="ACHAUN_001771"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8460468.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(12332..13627)
                     /gene="aroA"
                     /locus_tag="ACHAUN_001772"
     CDS             complement(12332..13627)
                     /gene="aroA"
                     /locus_tag="ACHAUN_001772"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8460469.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(13640..14731)
                     /locus_tag="ACHAUN_001773"
     CDS             complement(13640..14731)
                     /locus_tag="ACHAUN_001773"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8460470.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(14748..15914)
                     /gene="aroC"
                     /locus_tag="ACHAUN_001774"
     CDS             complement(14748..15914)
                     /gene="aroC"
                     /locus_tag="ACHAUN_001774"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8460471.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(15916..16980)
                     /gene="aroB"
                     /locus_tag="ACHAUN_001775"
     CDS             complement(15916..16980)
                     /gene="aroB"
                     /locus_tag="ACHAUN_001775"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8460472.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(17013..18035)
                     /gene="aroF"
                     /locus_tag="ACHAUN_001776"
     CDS             complement(17013..18035)
                     /gene="aroF"
                     /locus_tag="ACHAUN_001776"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8460473.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         5351 a         4010 c         3300 g         5797 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaattaaa agtatgaatt attcaggtta gaatcttttg ctgacgtcta
     1681 aaaatagaaa aagaattttc gtcatctatt tgataacgca tttgtttttg cagttctgct
     1741 gcagtcagat ggatcttccc atcgtttgta tcgatcatta aaccttctgg aaacagttca
     1801 accactgctc gatcccaaga ttcaagagaa agctcgatat aatcttttac tggtaattcc
     1861 aattctacct taaaaaagtg caaaacagcc gacagccctt ctgcttgata gatttctcct
     1921 aatgttagcc cctctagatg gaactctggt gaaaaatgca gcaatgtttt attttcctgc
     1981 aattgaacat atagaaatgt agatcgctca atcagagtat cttttaaaac aactaaaaaa
     2041 ttcatttttc tccccctttt ttcatcattt ttaaaatagc atattttgaa aagaaagtct
     2101 gatgcttaga gtagaatttc ataaaaattt actatttcat aagtattcac gagaaaaata
     2161 caaacaaaaa agcggaaata acgctcatcc gccttcttgt ttgtgtttat ttcctcttca
     2221 aacgcttgtc tatgattcag acttttctct atcgttattt tcatctaaaa gttcttgcat
     2281 tccgataatt tctcgatatt gatgaatctt agtgatacca atgactttgt ctacatcaat
     2341 actaaaacaa ataccattac ctggaatatc gatgttgatg ccttgaccaa tcgcatccat
     2401 tactttattc gtcaaactat cgggtactaa tgtcaacaca atatcttttt ctggcgaaat
     2461 gtccaaacct aaaaatttcc ctgaatcatg tacaccagaa cctcttccat gtaagatcgt
     2521 tccgcctgct gcacctgctt cttttgaaaa gtcgataaca tcttcgccca ttcctccgtc
     2581 aacgatcgtc acaatcatgt gaagattcaa tgctacaaga tctttcattt gctttccccc
     2641 aatttttgca atctttcttt ttcgattctg tctttttcat cttttcgttt ataaatatat
     2701 cctagtacta aagtggataa tattggtgtc aatgcaatca ttgcaaccac accaaacccg
     2761 tcaatgatcg gatctcttcc ttcaatttgt gcagcgattg ccacgctcat tgataaaata
     2821 aatgtcgaac acatcgggcc cgttgctact ccaccgttat caaaagccat tgctaaaaag
     2881 atcttgtcca cttttcgtcc taaaataaat gctagcccgt atcctgggat caagaaataa
     2941 taaagagaga atccgacgag caaacgccac atagacaatc ctactgctgc gccgatccca
     3001 attgaaatta ccatcagcat gattttcgat cggattctcc cctcgctcaa ttcttcaacc
     3061 tgtttgatca ttacatgaat cgccggctca gcaaacccta ctaagaaccc cattaaaaag
     3121 ccaaggggaa ccagtatata attgtaaggc aatcccgcaa tagcactgcc taaatgctgt
     3181 cctaccggta cataagccat attgactccg tgcaggaaaa ggatcaaccc aatagtcgtt
     3241 atcaagaaac ctttcataat gtcaaagaaa cgctttttgc gcatacgaaa actaatgata
     3301 ttcattaaaa caaataggat tacgataggt gccactgcca aaatgacttc catggtaacg
     3361 atttcgattc catctatcac ttgctgccag aaactcattg gaagatcacc cccaaaatca
     3421 tcacggcaag aatcggccct aacgaagcaa taccgaccat tccaaaactg tcattttcat
     3481 ttttcttttg tttgatcata ctagtcatcc ctccggcaag ggccaagata aatggaacag
     3541 ttactggacc agtagtcact ccccccgaat caaaagcaat cggcataaat tcggaactgg
     3601 tgaaaaatga ggcgatcaaa acgcctatgt agcctatagc catcaattga taataggata
     3661 gcttgaatac gacccgaagc agcgcaaagg ctagaaatac tccagtgccg acgctgacga
     3721 tcccaatcag caacactctg ccaatctctc cgtcagatat atcatatact tgttgtccca
     3781 aaacttgcac ggaaggttca gcaatcgtga tcacgatccc gatagcaaat cccaaactga
     3841 ccaaaacggt caaacttttc tttttgatca tatatttccc tactagatct ccgacttcca
     3901 tcatagaata atctgctcca aacaaaaaga gcgtcattcc gatcatcatg attgccgcac
     3961 caaccagaaa tgaagcgagg tcttctcctt ctaacggtgc aaaaacaaac gttaaaatga
     4021 caatcaggac agtcatcggc aatatagcag caatcacttc tttaaaattt tttagcatgt
     4081 atcctcctcc tttactcttt aagtttacct taaaatgaaa gagttttcga gcacaaactt
     4141 aaaacgttat catttaattt ttctcaacat aaaataacac acgtcccaaa agtatctttt
     4201 tacttggaaa aatgactttg aaggaacgcg cttattgtgt ttttttattt tacagagccg
     4261 atcattcctt gaacgaattg cttttgcaaa acaaaaaata cgatggcagt tggtaaagta
     4321 gcgatgacta atccagccat gatcattcca tagtctggtg tataggaagc acccatagca
     4381 gataaaacaa gcggaaccgt ccgtttatca ggagattgca aagctactaa gggccataaa
     4441 taattgttcc agctactcat gaatgtgatg attgcagccg cggcataggt atttttagcc
     4501 gtcggcatat acactctaaa aaaaatactt agttctttta gaccgtctaa tcgagcggct
     4561 tctactagat cttttggaaa agatttaaca ttttgtcgaa agaagaagat caaaaaagca
     4621 gtgctaacag atggcaggat gacaaccaag aaactgttga ttcccaaaac cgttccgttc
     4681 aattgtgaaa acattcgata taaagggatc atcaatgccg caaaaggaac catcatcgat
     4741 agcagtagaa tattgaaaat taggtctttt cgtttacttt gataaatctc aaatccatat
     4801 cctgccattg acgaaatcag caatgcaaac acagtggtca ccaaagcgat catcgctgaa
     4861 ttccaaaggg aacgagtaaa attcagctca ttggaaaaca aattttgaaa attgatgatc
     4921 aagttactgc caatctttat ttttcccgat gtgatatcca cgggtgtatt cgtcattccc
     4981 aaaaccatcc agacaaaagg gaaaatggaa ataaatgata ggatgcttaa tgctgtatac
     5041 tttaatactg acccaattgt cgaacgaagt tctaatgctt gttttttgac gttggggcgt
     5101 tcgattgttt ctttttctac catatttttc atcgactttc tcctcctact ttcgtttgga
     5161 ttatcgacaa tatcacaata aaggaaacaa taacaaagga aactgctgcc gcatatccaa
     5221 aattaggcgt gaatttataa ctcaaattat agatgtattg agatagggtg gtcgttgcat
     5281 ttgctggtcc cccgccagta atattttgta cttcatcaaa aagctgcaat gtcccgatcg
     5341 tcgaagtaat ggaagtaaac aaaatgattg gttttaaatt tgggatcgtc actttgataa
     5401 actgctgcca tttagaagca ccgtcaatag atgctgcttc ataaatctcc gaatcaatat
     5461 tttgcatgcc tgaaaggaaa aaaatcatat tgtatccagt ccaacgccaa gtgatcgaaa
     5521 gaatgatcag aactctcgcc caaattggat gagtcaacca gccaatcggt gtgtcaatca
     5581 taccaatgcc caacaacaag ttgtttacaa ggccagattg agaaaataga cttttcatga
     5641 tcagagaata actgaccatc gaagtgatac atggaagaaa gatagcggtt cgaaacaacc
     5701 ctttatattt caacttttta tcatttaaaa ggttggaaat aaaaagtgct agaaacaaca
     5761 tgatcggtac ttgtacaagt aaatacagga atgtattaaa aaaagctttt ttaaaagttg
     5821 catctgtcaa catccgctga taattaccta acccgttaaa tgtcatattg acagggggac
     5881 cactttgaaa agacgtgatc aaagccaaaa ccatcggaat aaaaaccatg attgatatca
     5941 aaatgactgg caagattaaa aaagcatgcc cgtttttgaa taaatctttc tgcttcattc
     6001 ttttccctcc aattctatca ttgattatct acatgaaaag acaagaaaga caatatctgt
     6061 aaggcagacg tgatcttttt tgtcttttcg tttgtctgat gaaaaagcga agaaattcac
     6121 ttttttacca ccgatcaatt agccaattgt gcttctactt gtttttgtgt atctgctagt
     6181 accttgtctg tctcttctcc attgatgatt cgatgaagag cttcagctac gactgcttca
     6241 attgcatagg tctcatgtcc gtaattcact tctggtattt cagatgtcca tgttgaaaaa
     6301 tcgtcaaaga tcttttgacc actgtaaaat tcagaagctt cttgataagc ttcttgttca
     6361 ttcgcgctaa gcatcgtaga tactagtccg atttctttcg caagagtgcc catcagttct
     6421 tcattcgaag caaacgttgc ttctaggaaa tctttcgctg aatcttctcc agctactcct
     6481 ttgatcacat accagccagc gccgccaagg ttagaagctt gcgctttttg cagattttct
     6541 ggaagtgcag gtattggtgc aatcttccat tttccagatt ggttttctgc tccttggatc
     6601 gttgaagaat accaagcgcc agttgggcta gaagctactg ctcctgattg tatcgcagtt
     6661 actccagcat tccagtcaga ggtttgttcg actagatctt ctttcaaaag agtggcaaac
     6721 aattcaagac catactttaa agatgcattg tcttggatcg tgactgtctc cccatcttca
     6781 gatgtatacc attctcctgc ctgttgcata atcatcctta ctcgaccaag gtcagaagga
     6841 ttgacttcag cgattttctt tcccgtcttt tctttgacat ctcgcgctac ttgaatatac
     6901 tcgtcccact tcagattctc catatcctct tctgtataac ctgcttctgc catcagatcc
     6961 gtacgataga agtttgctgt tacgcccgaa tcgaaaggaa caccgtatat tttatctcct
     7021 actttgttta ccgcaaattt atacggtgca aaatgatctt cctttacaat agcagacaaa
     7081 ttagcgaatg catctggata agaagtcaaa tagccttgta tcctataatc ttcaatcaag
     7141 acgatattgg gcaatccttc tgtattcccg ctcgctaaag cggtgttcag tttctgaacg
     7201 atatcatcct gagacatcgt aacaacttct atctctgttt cttcgttctc ataaactttt
     7261 tttgcttcat tcactgcttt gatattgaat gtctcatccc acgcccagac agtgactttt
     7321 tcttctttgt ctgctgtact actagcactt ccgcatcctg ttaagaaacc tgatgctgcc
     7381 gctaaacaaa cgactccagc aattaacttt ctgatgttca tttttatccg ctccttttcc
     7441 atccattagt tgtttacgtt tgctacattc atcataccaa aatttgaaaa cggtttcttt
     7501 attattcaga tacttcaaaa atttctccta aaataataaa aaaatacaaa tcaactatga
     7561 caaaaggaat aatcttagca tctagaccaa agatcgaaat ggtgaggttt ctttaaaata
     7621 aaaatatcct tgcattttct cctagaaata agtattttta aattacaaga ctattcttaa
     7681 ggcgggtaaa aaaatgccaa tacgcgtaat tcttatcgat gatgaacaga cgattttaga
     7741 tggaatgaaa cagttgatca attggtctga gcacgggttt caattaatag ggactttttc
     7801 cagtccgctg aaagcgcttg atttttcttt ggtacatgca atcgatatcg tggtcacaga
     7861 tgtaatgatg cctgaattaa atggaatcga attatcgcgg ctattaaaac agcaacaacc
     7921 tgaaacacag atccttatcc tctctagcta tgatgaattt gatatggtta aagattcttt
     7981 taaagaaggc gtcgctgatt atcttttaaa gcctcgattg agtcctgatt tttttctaaa
     8041 ttccttaact gccgtttcac aaaaaatcaa aaaaaagccc aacaaaagcc caatcaccaa
     8101 tcgttatgag cagatctgcg agaatctttc acagcagatt gctggacaaa ctcgtccatt
     8161 gcttgaccct cttctttttg aacacacctt gtttttctgt tatataccga aaaacggcca
     8221 ttcagttcaa aacaaggctc taaacaaaaa cagtcgatca cccattcatt gatggatagc
     8281 gacgattcgt ttactattta tcctgtttct accttttcgg aagaatccgg ctatgtaatc
     8341 aatactgatt ccaaagatac acttaggaag tttgttcaaa agtttcgtca agaagttttt
     8401 ccaggtgatt tttttgttgt ttctgattct tttgcaatcc aagatatcaa tgaaatattc
     8461 caagagatca gaaattgcac caaaggacaa cgtttctatc ataagggaaa gaaccttgct
     8521 tatcagcatg aacttcttcc gttattaacc aaccacgagc agcaagcaag tagttattta
     8581 acaaaaatcg ttcataaaga ctacgcttta gctgtcaaag aacttgagaa tttcttcacg
     8641 acgatattgg aacagcctat tgatccagct tatttgaaac atgaggcaat caatcgtttc
     8701 tatgcattac tcaacgcttt ggctgacgag catgttggga atgaagaact ttctttgctc
     8761 aagatcgccc ttccttcttt attagccgat gctgagtatt tcgaagattt ttctattctt
     8821 gtcaaagaaa cacttgagaa actcacccac ttttctgcaa atgcaaaaaa agacaatttc
     8881 gatatcttga ctttgatcta cgagttcgtc caggaaaatt atgagaaaga aatcaattta
     8941 caacttttat ccgaaaaata tcatttttca tatagctatc tctcagccat ctttactgaa
     9001 aaatttggta tcaacttttc aaaatatcta aaaaaagtcc gtatcagtaa agccaagctc
     9061 tttttgacgg aaacacggct tactttgacg gaaatctgtg agaaagtcgg ctataccgaa
     9121 ctgggttatt tctccagagt ttttaaagaa gagacaggaa tgacaccttc tcaataccgt
     9181 aaaggaactt ttgtaaaatg agaaaaaaaa tcaaagagca tgaactcttt gccaaacttc
     9241 tgttcatttt gttcatcggt ctttttctgc aagccatcgt catttctttg tttatttatc
     9301 atcgatcaag ggatgcctat atccagctgt tcaatcaatc gaatgatgtg gtactaaaaa
     9361 agattcaatc tgaatttgaa agtttgaatg atacaatcga gaatacatta gccgcttttg
     9421 attctaatcc agctgtcaaa agctattttt ccaatgatcc agcccaacat atggaacagc
     9481 ttcagcaact aagaacaatc caaaaaatga atgactctct ttcaaaaatc catccaatga
     9541 tcgattacga tgtattgatc tttggagaaa atggaagaac ttttgtcgga aacgacatgc
     9601 tcactgccgt atctgccgat tctttttttc aatctgcgat tgctcagaga gtgaatgaac
     9661 gtgctgctga tacacaaatg ctttttgcac accacggatt gactcttcgc gataagaaag
     9721 cacccagcgt cttctttgtc aaaaaactga aaaatacatt gaatcacgtt tacggatatg
     9781 ctgttttatc gatctcatcc aagcaactag cgaatctttt tcaatctgta gtaaatcctg
     9841 agatatcgaa gatctccttc atgaacgaag aacagacgat catcgcatca aatgaagaaa
     9901 atacaattgg gagccgatca atgattttca aaaaactaac agtaggtgaa acaacgattt
     9961 caaatggaca gcagttgacc cgtcttcctc tgtaccgcca ggattgtaca ttactcagtc
    10021 aagtcgatct tcattcttta tctaaccaaa tgggcgtgat ttttccgatt attttgtata
    10081 atatcgtgac acttgctttt gctggctcat tggtatttat gtatttggat cgacacacaa
    10141 aatcgatcta ccgcttgatt gattcgctaa aacggctaga ggaagatggc ttaaaagcta
    10201 ctgttcctgt acaaggaagc catgaagtcc aattgctagg gaatacgatc aatcaattgc
    10261 taagtaatgc tcagcatcat tatgaacaga cgatccaaga cgaaaagaag aaacgttcgc
    10321 ttgaaatcca aactatgcaa gcacagatac agccccattt tatttataat acgctgacta
    10381 gcttgaaatt tttgatttgg cagaaaaaaa cagatgaagc agttcaagga atcgacagtt
    10441 tcattacttt actaagaagt acgattggga aaaaagaaga agtgattcca gtcaaggaag
    10501 aaatcaaaag cgtacaaagt tacatcgatc ttttgagtat gcgatatggc gaaagaatct
    10561 cggctaaaat aatgattcct gatgaactgc agtcattatc tatgccgaat atgatccttc
    10621 aaccaattat cgaaaacgcc tatcttcatg cttttcacaa taaaacgcaa ggattcatca
    10681 cggtgtacgg caaaacaaaa aatcagcttc ttttattcga agtgattgat aacggcgatg
    10741 gcttcgatac tgaaaatacc aaacaaaaaa atgatcattt ttcaggaatc ggcatcagcc
    10801 atgtagatga gcggatacgc ctaatgtatg gcgatgcata tggcttaacg atccaatcag
    10861 tgattgggac aggaacgact gtaacgattt gtctaccgct tcttcctaaa aatcaatcag
    10921 aatatcaaaa ataaagaata gccaagcgct gattttttta ttttaagtta aataaatcaa
    10981 tcagtattct tgactttcat ctacgcgtct cttctactgt cagcactgga taacagccca
    11041 atatttgact ttctcctccg agcatcttga tttcttggag cgcattctct actaatgtca
    11101 ttggacggtc caacaacaaa tcgatcacga aaaaatattc tcccaagcta gttttcaatg
    11161 gtcgggattc aatcttactt aagttgattt cccgccagcc aaaagctgcc agcattttat
    11221 gaagcatccc tggccgatta gcaggtagtg tcaagatgac actcattttt actggagctg
    11281 actgctggct ggtcatcttt cggtcaccga cgatccaaaa acgtgtctgg ttcaactcat
    11341 tatcttggat attctcagct aagatctcca atccaacatg ctgtgctgtt tctaatgaag
    11401 caatcgctgc tgcgtcttct ttcggatgtt ctgctacata cattgctgca gcagttgttg
    11461 attctgttgc tactaaagga acattaggat aatgcgtttc taaaaactgt tgtgattgtg
    11521 ctagtgcttg tggatgtgaa aggatttttg taatctttgt tgctggattg cctaaaagct
    11581 gctgcttgat aggaagaacg atttcacttt taacttcgac ttctggatgt ttggaaagaa
    11641 gatcgattgt gtgatgaaca gctccttcca atgaattctc tacaggaacg acagccagat
    11701 cgacttgttt gcgaaacaaa gcgcgcaaac aaacagggat ggaagcatac gcagtcaatt
    11761 gttctgtagg aaatagctga caagccgcct gataagtgaa tgaactttct ggccctagat
    11821 agctgacgtt catatgcttt tgatcctttc tataatggta tcgatgatct tttctggtgt
    11881 ttgattggtc gtgttgattc gatgtgtcgc actttcttca taccaagaaa gtcgttgtgc
    11941 caataatgct tttatttctt ctcttgtttt tccaagagct aaaggtcgaa tagacgtttc
    12001 atcttgttgg atacgatcaa ctagacagtc cgcttctgcc tctaagtaaa gaacaatcgg
    12061 ctgtgttttt aaaaaacgcc gattttctgc actttggaca atccctccac cagtcgcgat
    12121 aattttgtct tcatttgaca gctttcttaa aatatctgac tccacttttc gaaagctttt
    12181 ttcaccgtaa tagtgaaaat attcggcaat cgacaattgg atatgttcct caatctttgt
    12241 atctaaatct acatactctt tttgtaaaca ttgagctaac cctttaccga ttgttgtttt
    12301 tccagcgccc atgaaaccaa tcagcaaaat gctagccatg agcttctcct cctgacacaa
    12361 gttctgctaa atcgtcaaaa aatgccggat aagagattga aacagcttca gctccttcta
    12421 gttcgcaagg tccatcagct atcaaagcag ctacttgcag catcataccg attcgatgat
    12481 cgcctctgct ggacactttt gcgccatgca gaggtgtcgg cccatggatg atcagaccgt
    12541 ctgctgtcgc ttttatcttt gctcccattt tctgtaattc ttctgccacc gcatcaattc
    12601 tgttggtttc ttttactttt aattcttctg cgtctttgat gatcgtgatt ccttctgctt
    12661 gagtagccac taaagctaga atgggcaatt catcaatcag acgcggaatg atttctccac
    12721 caatcgttgc tttcttcaaa tgactcgtct tgacacttaa atcagccgat tggttctgtt
    12781 cattgtgatt cgtctgggta atcgctgcgc ccatctcctc caacacatct aagataccag
    12841 ttcttgttgg attgaccccg acatttttta acaacagttg gctttccgga acaagaagtc
    12901 ctgctgctag aaagaaagca gctgatgaaa tatcacctgg aacagttatc tgttgaccag
    12961 ttaatttttg cggtccggtc actatgatcg ttttatcttc cactgtgatc cttccgccaa
    13021 attgacgaat catttcttcc gtatggttac gggaacgttc tttttcaacg attacggatg
    13081 tgccttctgc ttgtaatgcc gcaaacagca aagcagattt gacttgcgca ctggctacag
    13141 gcatattgta tcggataggt gataaagagc gggttcctgt cacagtgatt ggtagatact
    13201 ggtcattttc ttctccttgc aagtcagcgc ccatctcgcg taaaggttcc atcactcgcc
    13261 ccattggacg tttagacaac gaagcatccc cgaacagagt agtcgtaaaa ggctgtccag
    13321 ccaaaatacc catcaataaa cgtatggtcg taccggagtt tcccatgtca attgattcgg
    13381 ttggttggac aaatgagtgg ctaccgcgac catgaatctt gatcacctct tcctcttctt
    13441 cgatctcgac acctaattgt tggaaggcat gtaaagtact cagacaatct tctgcgcgca
    13501 aaaaattttg tatagtcgtc gttccttcag caattgctcc aaacatgatg ctgcgatggg
    13561 aaattgattt atctgctggt atcctaacag tccctcttaa tccatgtatt tgttgtaata
    13621 attgcattta tccagcctcc tatttgatcc agcaatgata actggtatta gacataatga
    13681 ttctttttgc ttgtaacaaa tcctgttggt ttttaaaact gatttgcagt attcctaaaa
    13741 tatcttccct ggtctcttgg atcttcaaat taatcaaaga aactcccgct tttccgatca
    13801 gtccagttac ttctgcaata acgccaggtt catctggcac atcgataaat aaatcaaaaa
    13861 aggcaggaat cgcgccttgc ttatggacag gcaactggtc tcgactttct tttgcccgat
    13921 tgaaaaaaga aaagatttcc gattggttct cagtcatgat ccattctgct atttgtgtca
    13981 tttcttgttg ccaatctgcc aatcgttcca ataatatctg cttattgctc aacaaaatat
    14041 ctgtccacat gacaggatct gaggaagcga tcctagtgat atctcgaaag cctcctgctg
    14101 ccagttgttt ggctcgcgga tactcttgat tgaattgttc tgcctgattg actaagccag
    14161 ctgccagaat atgtggaaaa tgacttagca ttcctgttat acggtcatgt tctcttgctg
    14221 taagagttac atattttgct ttggttccgc aaaatagttc ttgcagtttt tcaacatctt
    14281 ttttcgtctt ttccaaaggt gtaaagatgt aataggcatt ttcaaatagt ttagcatctg
    14341 ctgcctgtat acctgattta tgagaaccag ccattggatg tccaccgaca aaccgaagat
    14401 ttttctgctc agcggatgtc acgacttgct gcttcgtact gctgacatcc gtaatgatga
    14461 cgtgatcaga caagttgatt ctttcaagga catcaagata agctaaggtt gtacggacgg
    14521 gaacggctaa taagataacg tctgcttgtt ctgcacctgt ttcaaaatca cttgcaacgt
    14581 aatcaacgat ctgctgttct actgcttgct cacgggtttt tgactgacta tcccaaccaa
    14641 ctactatcag ttctggattg gtctgacgaa tacaaagtgc catagaagca ccaatcagtc
    14701 caagccccac gataaaaaca tgttgtgcca tcttatttgc ccccttttta gtaatgtttc
    14761 aagtagtcac ggtactcctt cactgcagct ttcaactcaa caagcgaatc acttgggaat
    14821 tttgacaata gctctgtcgc taccacagtg gctacaacat tttccgcaac gacgctagct
    14881 gcaggaactg ctgtactatc cgaacgttct acacttgctt tgtaaggttc ttttgtatcg
    14941 atatcaacac tttgaagtgg cttgtacaat gtaggaatgg gtttcatgac cccgcgaatg
    15001 acaagtggtt cgccattggt cattccgcct tcgaagccgc ctagattatt ggtacgtcgg
    15061 aaatagcctc tttcttcttc ccataggatc tcatccatca cttctgagcc tggtaatgaa
    15121 ccagaagtga atccgacccc aaattctacg cctttaaagg cattgatact aaccacagct
    15181 tgtgcgattt tggcatctag cttcgtgtcc cattgtacgt agcttcctaa tccagctgga
    15241 acgcctccaa cgagcacttc tacaattcct ccgatcgtat ctccattttt ctttgtctga
    15301 tcgatcaaat cttttattct ttgctcaact gctggatcaa caactcgtac ttctgacgct
    15361 tcagaaattt ctttgatttc ttgaatcgag atatttccag gtatttctgc ttggattcca
    15421 ccaagacaaa tcacgtgtcc tgctacgatg atgtcaagtt cttccagaat tttcttggca
    15481 attgcgccga ttgctacacg catggtcgtc tctcttgcag aggaccgttc aagcacattc
    15541 cgaagatctc gatgatggta tttcataccg cctaccaaat ccgcgtgtcc tggacgcgga
    15601 cgtgcaactc gccgcagtga ttggtctttt tcaggcactt ctgctacaga catgacagtt
    15661 gtccaatttt tccaatcttt attttctacc actaaagtga caggtgaacc aattgttttg
    15721 ccgtgacgga tgccagacgt gatccgaaca cgatcttttt cgatcttcat ccttcctcct
    15781 ctgccgtaac cagcttgtct gcgagcgagt tcgtgattga tttcttcttc tgacagaggt
    15841 aatcctgctg gtagtccttc aataatggct gttaattctg gtccatggga ttctcccgca
    15901 gtcatataac gcatcttaca gtcctcctaa gtaatctttg aatgtctctg tgggaacagg
    15961 aacgattttt gcttgcccaa tcttttctag caagatgata ttcaattggc ttcctcttgc
    16021 tttcttatca tgggttatcg cgtggaataa ttcatctgga tgacttagat cgtacgtaat
    16081 cggcagatgg aatttttgaa gtatcctttc taaagcagct gtcgatccag ttggagacaa
    16141 gccgattttt tctgcatgag aagtgatggc taccattcca atagctactc cttctccatg
    16201 actgactacc ccatatcctg ctgtattttc aattgcatgg ccaattgtat gaccaaaatt
    16261 taaaataagt cgatttcctt ggtcaaagac atcttcttcc actacttttc gtttgatttc
    16321 caacgtagca gtaatgattg gaacagcatg ttccagaaga tctttttcgt ctttcagctc
    16381 gttcagctgt ttccaaagcg ttgcatctgc tatagctgct gatttgatga tttcagcgat
    16441 tccttctcgt aaacgacgta ccggcaaagt ttttagaacc tcaggatcaa tcaatacacc
    16501 agctggctgg ctaaatgttc cgacaaggtt tttcgctttg tttgtattga ctgctgtctt
    16561 cccgccaata ctagaatcta cttgtgcaag tagcgtagtc ggaatttgaa cgaaatggat
    16621 cccacgcatg aacgtcgagg ccacgaatgc tgctagatca cccaccattc ctccaccaag
    16681 tgcaagtatc ccatcgctgc gggtaaaatc attatctgct aaaaaatcgt ataactcagc
    16741 tgcttgttct aaagatttac tggtctcacc cgccggtacg attgaaagga ctggttcaaa
    16801 attagcttgt ctcaattgga acatgatttt ttctgcatat agtggagcaa cgtttgtatc
    16861 agtgatcaca gctacttttt gaggtctcca aatcgtttga agccatgatc cagcctgttc
    16921 tagtgcgcct ttttcaatat gaatatcgta tttttggtta tcaacatgta cagtaagcat
    16981 tcgtttcact tctttctttt aaaaagctgg aattaatcga gtgtctgaat cttttccatt
    17041 gcttctttca tgatcgcaac ttctttcatc atgcgtgagt aagtcttttc attcaacgat
    17101 tggggaccgt ctgaccaagc atttgcagga tcaggatgga tctctacgat catgccatct
    17161 gctccgcttg ccacacccgc acgagccatt ggtggtacaa gttcccaaat tcctgtacca
    17221 tgacttggat caacaatcac agggaaatga gttaattttt tcatcaatgg gacagcactt
    17281 aaatcaaacg tattacgtgt cgctgtttcg taagtccgaa tcccacgttc gataaaaatg
    17341 acaggagatt tgtcttcgac tgcaatgtat tctgcagcat taagccattc attgatcgta
    17401 ccggaaattc cacgtttcaa tccaacaggt ttacctgttt taccgacagc agatagcagt
    17461 ttaaaatttt gcatattacg tgcgccaatc tgtaggatat cgctgtactc agccaccata
    17521 tcgatatgcc cttcatccat cacttcagta atcactttca ttccaaattc atcagccgct
    17581 tgacggatgt atttcaatcc ttcttcttct aaaccttgga aagcatatgg agaagtacgt
    17641 ggtttgaagg caccacctcg cagaattttt gcgcccccgg ctttggccat tcgcgcacat
    17701 tctctgattt ggtctaatcc ttcgatagaa caaggaccag ccatcgtcac gaaactgccg
    17761 tcaccaattt ttactccgtc tacatccacg actgtattgc caggatggaa ttctcgagaa
    17821 gtcaatttgt atgtgtttaa aatacgcaca gcattttcta ctccttcata gcgtgtaaat
    17881 gctccttctt gtaaacttcg cgtatcacct tttagtccaa tcactactcg ttctgttcct
    17941 tgatcgattg agactttgaa tccttctttt tcgatatgat cgacgacttt ttttatttgt
    18001 tcttccgttg cttctttttt cataattact atcattgttt ttcccgcttt cttttgttta
    18061 cttatctatc ttggctgatt ttctctactt tttcaaggtg gttttacatt aattcctact
    18121 aaatgaccgt gtcctgttgt tctcattttc gctccttctt tcctacttat gacccagata
    18181 atcaacaaaa aaagatgtcc ctgtaggaaa ttgcctctac agtgaacatc ttcaacgagc
    18241 cttaaaaagc gctgcaaaaa tataaaatag ccactataga tgaatttctc tacgtggctg
    18301 taagtcgtgt ttaaaaatct ttagccatca tagatacaaa cctggtttgt ttgtacccac
    18361 gaagtcatga atacaaacct atctaaaata gctaaagtaa tacgtattca gttttgtaag
    18421 ttttctagat ttcataaaaa atcgctccaa actgatgt
//
LOCUS       DAYYBG010000055        18436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000055 DAYYBG010000000
VERSION     DAYYBG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.056"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..516
                     /locus_tag="ACHAUN_001777"
     CDS             172..516
                     /locus_tag="ACHAUN_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8460474.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ACHAUN_001778"
                     /pseudo
     CDS             517..936
                     /locus_tag="ACHAUN_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="ACHAUN_001779"
     CDS             complement(1028..1378)
                     /locus_tag="ACHAUN_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460475.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ACHAUN_001780"
     CDS             complement(1344..2675)
                     /locus_tag="ACHAUN_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8460476.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="ACHAUN_001781"
     CDS             2931..3497
                     /locus_tag="ACHAUN_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8460477.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="ACHAUN_001782"
     tRNA            3642..3729
                     /locus_tag="ACHAUN_001782"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="ACHAUN_001783"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="ACHAUN_001783"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8460478.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="ACHAUN_001784"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="ACHAUN_001784"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8460479.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="ACHAUN_001785"
     CDS             complement(7452..7898)
                     /locus_tag="ACHAUN_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8460480.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="ACHAUN_001786"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="ACHAUN_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8460481.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="ACHAUN_001787"
     CDS             8363..8881
                     /locus_tag="ACHAUN_001787"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8460482.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="ACHAUN_001788"
     CDS             complement(8958..9914)
                     /locus_tag="ACHAUN_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8460483.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="ACHAUN_001789"
     CDS             10080..10886
                     /locus_tag="ACHAUN_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460484.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="ACHAUN_001790"
     CDS             10883..11872
                     /locus_tag="ACHAUN_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8460485.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="ACHAUN_001791"
     CDS             11869..12873
                     /locus_tag="ACHAUN_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8460486.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="ACHAUN_001792"
     CDS             13005..13547
                     /locus_tag="ACHAUN_001792"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8460487.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="ACHAUN_001793"
     CDS             13567..14265
                     /locus_tag="ACHAUN_001793"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8460488.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="ACHAUN_001794"
     CDS             14286..14498
                     /locus_tag="ACHAUN_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8460489.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="ACHAUN_001795"
     CDS             14498..15460
                     /locus_tag="ACHAUN_001795"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8460490.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="ACHAUN_001796"
     CDS             15478..15873
                     /locus_tag="ACHAUN_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8460491.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="ACHAUN_001797"
     CDS             16036..16401
                     /locus_tag="ACHAUN_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8460492.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="ACHAUN_001798"
     CDS             16398..18209
                     /locus_tag="ACHAUN_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8460493.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..>18436)
                     /locus_tag="ACHAUN_001799"
     CDS             complement(18271..>18436)
                     /locus_tag="ACHAUN_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8460494.1"
                     /translation="KKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENR
                     KKAMRKRVSR"
BASE COUNT         5924 a         3120 c         3810 g         5582 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttc
//
LOCUS       DAYYBG010000056        18123 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000056 DAYYBG010000000
VERSION     DAYYBG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.057"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      40..206
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            354..911
                     /locus_tag="ACHAUN_001800"
                     /pseudo
     CDS             354..911
                     /locus_tag="ACHAUN_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            969..3011
                     /locus_tag="ACHAUN_001801"
     CDS             969..3011
                     /locus_tag="ACHAUN_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8460495.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADDKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAE
                     IKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3120..4271)
                     /locus_tag="ACHAUN_001802"
     CDS             complement(3120..4271)
                     /locus_tag="ACHAUN_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8460496.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4425..4955)
                     /locus_tag="ACHAUN_001803"
     CDS             complement(4425..4955)
                     /locus_tag="ACHAUN_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8460497.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5054..5404)
                     /locus_tag="ACHAUN_001804"
     CDS             complement(5054..5404)
                     /locus_tag="ACHAUN_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8460498.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5561..7090)
                     /gene="ahpF"
                     /locus_tag="ACHAUN_001805"
     CDS             complement(5561..7090)
                     /gene="ahpF"
                     /locus_tag="ACHAUN_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8460499.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7100..7663)
                     /gene="ahpC"
                     /locus_tag="ACHAUN_001806"
     CDS             complement(7100..7663)
                     /gene="ahpC"
                     /locus_tag="ACHAUN_001806"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8460500.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7807..8469)
                     /locus_tag="ACHAUN_001807"
     CDS             complement(7807..8469)
                     /locus_tag="ACHAUN_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8460501.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8653..10299
                     /locus_tag="ACHAUN_001808"
     CDS             8653..10299
                     /locus_tag="ACHAUN_001808"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8460502.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10290..11372
                     /gene="uxuA"
                     /locus_tag="ACHAUN_001809"
     CDS             10290..11372
                     /gene="uxuA"
                     /locus_tag="ACHAUN_001809"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8460503.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11480..13033)
                     /locus_tag="ACHAUN_001810"
                     /pseudo
     CDS             complement(11480..13033)
                     /locus_tag="ACHAUN_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13181..14322
                     /locus_tag="ACHAUN_001811"
                     /pseudo
     CDS             13181..14322
                     /locus_tag="ACHAUN_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14448..15527)
                     /locus_tag="ACHAUN_001812"
     CDS             complement(14448..15527)
                     /locus_tag="ACHAUN_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8460504.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15636..16076)
                     /locus_tag="ACHAUN_001813"
     CDS             complement(15636..16076)
                     /locus_tag="ACHAUN_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8460505.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16133..16543)
                     /locus_tag="ACHAUN_001814"
     CDS             complement(16133..16543)
                     /locus_tag="ACHAUN_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8460506.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16724..17158)
                     /locus_tag="ACHAUN_001815"
     CDS             complement(16724..17158)
                     /locus_tag="ACHAUN_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8460507.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17258..18106)
                     /locus_tag="ACHAUN_001816"
     CDS             complement(17258..18106)
                     /locus_tag="ACHAUN_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8460508.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         6218 a         3359 c         3282 g         5264 t
ORIGIN      
        1 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
       61 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
      121 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
      181 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
      241 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
      301 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
      361 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      421 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      481 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      541 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      601 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      661 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      721 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      781 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      841 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      901 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      961 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
     1021 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
     1081 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
     1141 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1201 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1261 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1321 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1381 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1441 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1501 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1561 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1621 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1681 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1741 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1801 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1861 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1921 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1981 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaata gcttttgaca
     2041 gtctaggagg aaaagctgca tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     2101 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     2161 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2221 atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac aacggcacta
     2281 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2341 gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2401 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2461 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2521 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2581 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
     2641 ttaccaacaa tggcacactt gtctatccta aattgattgc agatgataaa gagacaaaag
     2701 ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat ctgagagaag
     2761 ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt ccgttggcag
     2821 cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa gagaacagtt
     2881 tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg ttggaaaata
     2941 aagaagatga tgattcagca accaaacgag catcggaact attgcaatac ctcaaccaaa
     3001 attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt atcgtcacag
     3061 tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt ttgatttact
     3121 cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat aagtgtactt
     3181 aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg actttgctgt
     3241 ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc accgaccatc
     3301 aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc aagcaatttg
     3361 gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact aaagtcagaa
     3421 aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc atatcctaaa
     3481 gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc tatcccagca
     3541 aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga cattcctaaa
     3601 caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc tgcaaatgaa
     3661 agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata aattagctgt
     3721 tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag aaaagatagg
     3781 ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc caaaattgtt
     3841 gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga aatactgaca
     3901 gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac ttggaatgct
     3961 ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc aacgaacata
     4021 ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc taaaaacatt
     4081 aataagatca caccaatctc cgaaaagtca tggactagaa tatcaggttt gacccaattc
     4141 aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc tgggatacct
     4201 aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca taaaatacca
     4261 ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag cataacatac
     4321 attattgaaa aaataggaaa atatactcac aaaaaaacca acaatccaaa agattttttt
     4381 ggattgttgg tttttatacg gtctatttta ataactaaaa atgattaacg tatagtcatt
     4441 tttcttattc tgatgaaatt gataatctcc tggatgccca tgaaaatcaa aataaagccg
     4501 aatatttgga atagaagcat caaacttttg aatggattga acaataagac gaatccgcca
     4561 atcaacaaca gaatactact ataaacactc caacttttag cttttgtatt catgctgaag
     4621 aacaactgga acaagctgtt gatgataaca gctaatccaa gaagaatcgg caggatagaa
     4681 acgattgcag gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa aagaccactg
     4741 aacagaccga tactccaaga acctgttttc cttttaaaac ggtaagcctc aaaaagattc
     4801 aacaacccaa gcaaaacaag ataggcggtg atcagataac cgataaaatg aaaaacagca
     4861 gaagggttta gcacaatcgc aatcccagaa ataatataaa cagcagctct taataaggcg
     4921 tatttttgaa tattttgttt aatcgtctca aacatataag aaaacctcct ttgacttatt
     4981 ttaacgtgaa ctagtcattt taccaaatta aatgttacaa aatgagcgaa ggagaagaat
     5041 atctctggaa aaatcaactt ctgaggatct tttccatttt tttgcctttt gctagctcat
     5101 cgatcatttt atctaaataa cgaatttctt tcatcaagtt atcctcgatg tcttctactc
     5161 gtacaccaca aacaacacct gtaatcaagg aacgtgaagg attggggaca ggggcttccg
     5221 caaaaaattg ttcgaaagtc acttgttttt gtagttgata ttctagttct gtttgggaat
     5281 aacccgtcag ccaacaaatg atttcatcta cttcctcttt tatacgatct tttctctccg
     5341 cttttgctac atacatagga taaacgcttg caaaacttgt cgtaaaaata cgattttgcg
     5401 ccataatcag tcacctttct ttttttcaaa ttatttacta cttatagtat ataagaaaaa
     5461 gagaagaaaa agcgattatt caaaaaaaca aaaaggattg tgacaaaatt cttgtcacca
     5521 tccttttttc cgctctctga ctttttacaa tcttcatagc tcaagtgcgc atcagataat
     5581 cgaatgctcc taaagctgca gtagctcctg aacccattga aacaatgatt tgtttgtatg
     5641 gactatctgt acaatcacca gctgcgaaaa cgccggggat attcgttgcg ccatttttct
     5701 cggtcactat ttcatttctt ggattcattt cgatcgttct atctagccaa tccgtattag
     5761 ggagtaagcc gactagaatg aatactcctg aaacagctag tgtatgcatc tccatcgtac
     5821 ggcgatcgat ataggagaga gattctacct gattcgtacc gctgatttct ttcgtcgctg
     5881 catgtttgat aacagtgaca ttcgcaagag agactagttt atcctgtaag atttgatctg
     5941 cttttaattc tggtaagaat tctagaacat atacatgttt tgctaaccca gccaaatcga
     6001 ttgctgcttc aacaccagaa ttaccaccac cgataacagc aatctctttt cctttaaata
     6061 aagggccgtc acagtgtggg caataagcaa ttcctttatt tttaaactct ttttcgcctg
     6121 gtacattgat agaacgccag tgcgcacctg tcgcgatgat ggcggttttt gctttaagtg
     6181 atataccgtt agctaagcca atattaataa aatgttgctc tttatttaag gaaacggcct
     6241 gttgattcat cataatgtcg atagggtaac tacgcacatg actttctact tgctgcatca
     6301 attttggacc ttctgtgtaa gaggtaccaa tcatattttc aatccctagt gtttcaacga
     6361 cttgtccgcc gaaagaatca gcgacaagtc ctgtacggat tcctttacgt gctgcataga
     6421 tagctgcact actagctgct ggtccaccgc caatcactaa tacatcataa ggttcttttt
     6481 caaacaactc ttcttcaatc aaagggccag tgattttttc aacaatttgt tcgatcgtca
     6541 tgcgtccgct actaaattct tctccattta aaaacacagt tggcactgcc atgatttttt
     6601 tatcatcgat ttctttttgg aacatcccac cttcaatcat cgtatgagta atatttggat
     6661 ttaatacagc taatatattc aatgcctgta caacgtcggg gcaattatga caagtcaagc
     6721 taacataact gtcaaaatgc aatgtttgtg ttattttttt gattttaaga ctcaaagatt
     6781 cactgatttt cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa gaggaaaatt
     6841 catgaccgag ggggatacca gcaaaaacga ttccactttc tttgccaact ggattgatag
     6901 aaaaactagg tgtgcgcatc gaatctcttt tttcccaaga gattttagga gacatttctg
     6961 ctatttcctt cgtaaattca gctagttttt gactattttc actattatcc aaactaacag
     7021 aaaaaacaat aggagattca atcagtgcta aatattgttc gagattttgt ttagtttctt
     7081 tgtctaacat atccaccacc taaatctttc caacaagatc aaaacttggt ttcaatgttt
     7141 cgccactttc tttccatttt gccggacaaa cttcacctgg gtgggtacgt acatattgag
     7201 cagcgcgaat cttatcaatc agtgcactag cgtcacgacc gattccatct gcattgattt
     7261 ccatagcttg aacgattccg tctggatcaa taataaatgt tcctcgctga gctaatcctt
     7321 gctcttcatc caaaacatca aaaatacgag aaatctgatg agacggatca ccgatcatca
     7381 cgtattcgat tttaccgata gcatctgatg tatcatgcca tgctttgtga gtaaagtggg
     7441 tatcggtaga gcaagaaaag acttcaacac ctaaagattt caatgtactg tattgttctt
     7501 gtaagtcttc taattcagta ggacaaacaa aagtaaaatc agctggatag aagcaaagaa
     7561 tactccagtt ccccataaga tctttttcac tgacttctaa aaattctcct tgatgataag
     7621 catgtgcatt aaattcttcg atttttttcc caatcaatga catagtaaaa cctccaataa
     7681 taattattta tttataatta ttgtaattta gttggagttg caaagtcaat ggatattttg
     7741 ttatttaata aagattactc aaaaacgaag gttaattata aaaatagaag aggtcgcaag
     7801 aaaatgctac tgctggaaat actcgggata acgttgagta acagtttcct gttctttcag
     7861 catcatgtgt aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt tatcaactaa
     7921 agccttgagt aaatcataat gttgttccat gatcgtttcc catggaagcc cctgaatggt
     7981 cactcgtaac cagcggaaac gttctaaatg ggtattatgg tcatctaacc agttccaaac
     8041 ttcttttctt cctgcaattt cgaaacaagc ttcatgaaat aaattatctg cacggaaaaa
     8101 agcgcgttcg tcatttattt tgactgcttt ttcttgttct tctaaaatcg tttctagcac
     8161 tattcgtgtt tgtgtgtcca atttggaaca acattcaatc atgatttgct cttccaactg
     8221 ttctcggaca aagcgagcat tttctgcaga aacaagatta attttggaaa catacgtacc
     8281 actttgaggt atcgtataca cgagttcttg ctgacgtagt tgaatcaatg cttcgcgaat
     8341 gggagtccgg ccgatagcaa gatgttcctc cagtgccttt tcagatattt tccttccagg
     8401 ttctaagtca gcatagataa tttgtttacg aatcgcttga taagcttgtg attgcaagct
     8461 gcttttcatc tttattcctc caaaatcatt tctatgaacg tagtataaca gaagaggtct
     8521 gaaaaacaat ctggtatgtc acattaacta gtaaatttgt ataaatagta aaattttggt
     8581 tgacatagcg atttttattg cataaaattc acttgaatac aaaaactaat atgttagttt
     8641 aggaggggaa aaatgattga tttaaacaac tggaggaact ttatccctga attatctcaa
     8701 aaagaagtag aaatacctca ttttgaccgt gcatcattaa gagaaaatgg acaaaaaaat
     8761 cctgtctgga tccatttcgg tggcggaaat ctttatcgag gatttcatgc agaaatcgct
     8821 caacgtttag cggatctagg cgaattgtcc actggtgtca ttgtttgtga aacattcgat
     8881 gaagaaataa ttgaaagggc ttaccaagat tttgatgatt tgattcttga agtggtcatg
     8941 ccaagcagtg gagagttcgt caaacgtata ttagctgcga cagctgacag tctgtattgt
     9001 catccagatt gttctgaaca ctttgataag atgattcgac actttgaaaa tcctagttta
     9061 caattagtta cttttgctat tactgaaaaa ggttatcgat tgaaagatac aaataataaa
     9121 tatataggac cgattgcagt cgatataaaa aatggtccaa agaagccatc acatacaatg
     9181 agtattcttg ctgtgctact ttataaacgt tttttatctg gagcttggcc gattgcactt
     9241 gtctctacag ataatttttc acaaaacggt agtcaattac gtacttcttt gctaacgatc
     9301 gcaaaagaat ggcaaaagga aggttatgta caaaatacat ttatccaata cttatcgaat
     9361 gaagaaattg ttgcgtttcc atggacgatg attgatcgta tcaccccgaa tccttccttg
     9421 tcagttagca aaatgttggc aaaagagggc ttttcaaata tgtcgatttt ccaaaccgca
     9481 aaaggaaata atattgctcc atttgtcaat acagaggaca tccattattt aatcatggaa
     9541 gatcattttc ccaatggacg tccgaaattc gaaaaagctg gggtattctt gactgatcgt
     9601 gcaacggtag ataaagcaga tacgatgaag gtgacgactt gccttaatcc tttgcatacg
     9661 tctttagcta ttatgggttg tttactgggg tataggagca tagctgagga gatgaaagat
     9721 ccagatttat cgctgctagt ttctgaaatc gggtacaatg aaggcctccc agttgtggag
     9781 tctccaggta tcattgatcc aaagatcttt ctggaagaag taattactgt gcgcttttct
     9841 aatccaaata ttcctaatat gcctcagcga atcgccacgg atacctctca aaaggtggcg
     9901 attcgttatg gtgagacgat taaaaaatat atggaaatag aagttaaaga cccgcagaca
     9961 cttgtattca taccattgac tctcgctgct tggcttcgat accttattgg cattgatgat
    10021 caaggaaaac cattcatccc gagtccagac ccactattga cagaccttca gcataagctt
    10081 tccgatattc aactgggtac gcaagataca tcacatattc atgagaaaat ggctcctatt
    10141 ttgtcaaata aagaaatttt tggtgtagat ctttaccaag cagagcttgg cagaaaaata
    10201 gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac attacaggat
    10261 tacttacaag aacatggagg aaatttatca tggaaatgac ttttagatgg tacggatcac
    10321 aggaagaaaa gattacctta gatgaaatca gacagattcc tgcggtcaaa gggattgtag
    10381 gcacattgat ggatatacca gtaggagagg tatggcctag ggagaggatc aaagaactaa
    10441 aagaagaaat tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc aatgtgcatg
    10501 atgccattaa aattggatta ccttcccgtg agcagtatat cgaaaactat aagcagacaa
    10561 tccgaaactt ggcagaatat ggaatagaag tcatctgcta taattttatg cctgtatttg
    10621 actgggtgaa atctgacctt gactaccgct tagaagacgg ctcatccacg ttagctttta
    10681 tatctgatga tattccagat gatccaagag aaatcgtcgc gcgcatcgaa cattccagca
    10741 atgaatttgg attgccggga tgggaacctg agcgcttagc tcctatcaaa tccttgcttg
    10801 aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat tttctccaat
    10861 caatcatccc aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca gatgatccgc
    10921 catactcgat ttttggatta gctcgaatcg taaagaatcg agaagattta gattggttgt
    10981 gtcgcatagt agacagccct tataacggaa tcactctttg tacagggagt attgcagaag
    11041 atccagaaaa tgatgtatat gggattttag cagaattttg ccaaagagat cgaatcccat
    11101 ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga tttctatgag gcaccacata
    11161 aatccgaata cggttcatta gacatgtacc gcatactaaa aacaatgtat gacaacggat
    11221 ttaacggata catccgtcct gatcatggaa gaatgatctg gaatgagaca ggaagaccag
    11281 gatacggctt atatgatcga gcattaggtg ctgtttatct aaatggatta tgggaagcaa
    11341 tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa actgtaattt tattatgtaa
    11401 ataagaagga tgttcaaatt cttgatacat aaatccggag gttgttaata agcattcgga
    11461 tttatgtatc ttatttggtc taggaagtaa cttgattttc ttttttcgat tgatccatga
    11521 gtgcttcttc ttttaattca aagatatatt ctgagatcct cataatcact tttttccagt
    11581 cttctggtgt gatgattcct tttaaaatat agattttttt gatatgagaa ggcaattgtt
    11641 tcaaagggca gtcgctgata acgatgttgc agttctccat aaaatcattt tgtattttga
    11701 tcgattcatt tgaaaaaatt tgttctatct taacgatcag atctttactg atataaaaat
    11761 cacgcgtata gttcacacca atcacaattc gcggctgttt tttgtagcga tcatacaaat
    11821 gtactaggtc ttcaaccatc cattgcacat tttccttttc taaaataact ggtaacaagt
    11881 gttgattttg ttctctgaaa tccatataaa attgttcaat ggcggtttgg acttctttca
    11941 atacgctatg ttcttgataa agcatgaaag taggaagggg cgtatttggt tgattgaagt
    12001 taagaaatcc attataaagt ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt
    12061 tttcaggtat aaacaaatca aatacagtac aaagggactc caccatttct cttgaataaa
    12121 gaacatgagg aacgtttgct ttttttaaaa gtttgtattg ctccaagctt gtttcttccg
    12181 attcgatttt cgtgatagaa agtcgagcta aaatattcac taagatgcgt tgttcgcgac
    12241 aaattggcag attaggtcgc aaaatatcta cagagagatc aataaaaaag tcaatatatt
    12301 catactctgt gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac
    12361 atatttttaa tagaaaatat aatttatcta tcataccgct atttagtcgg tcaagtactt
    12421 cttttttaaa aaaagtattc atcataaaag ccactacttg cggatcttca tcataaaatt
    12481 ctgtagcagt acttgaaaag atgttccaat aaatatctaa tgctgcatat tgaatttgta
    12541 cctcttttcc agaaaaagac tttctgccat tattagaaaa ggagatagac aaaccgtact
    12601 atgctaaaaa tgcattaatc tttttcattt tcgtatacaa ataagaggca ctatagtttg
    12661 tttgctgact gaaactgata atcgaatttg tctgacagct gaacaaagaa gtcaagatct
    12721 gataactgac tgattcgttg cagtatcttc caaatagacg ataatagcag taagccggat
    12781 caattccttc aaaattcaac tttaaaaaag gtttttgctt atcaatatgc agttctgtta
    12841 gattgagacg ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa tttgattcag
    12901 acatatggaa atgatctgct aaatattgtg tagtaataaa gaaacgatgt tcagatagga
    12961 aaagaaaaat atctaatttt gtattgcttt ttttcttcaa gataaacgtt ttaatcgctt
    13021 cttgagtttg catgaaagag gctccttttt acttaaattg ttaaaacttg aaaatattac
    13081 tgtttttttc ataaatttag acaaaaaaac agtaaacata taggtagaat ctctactatc
    13141 ttatttcttg catacaaaaa aatcaaggag tgagagagtt atgagatatt caaccatctt
    13201 aatatcaaca tttgcagcct taagcttatc gcttactata aatcagacag tccacacaga
    13261 ggagacagaa acagccaaat ggacagaaac caagtgggcc aacgtcaatc gaattacctt
    13321 tcaggatgaa ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc
    13381 gaccatcaaa gcaaaaattg actattcggg agacttagca cctatactac aatcagctgt
    13441 tacatttgag aatgttgatc ccactttgtc gattggctat gacgagaaca ctttgactat
    13501 ccaacacaag caagcgatat ttaccttcac cgttacatta aatcaggact tgttaaaacc
    13561 agcgcttttt actgtcaagg tatcagatgg actaccgaac gatcatcatt taacgccgta
    13621 tagtcagcgg cagacaattg agcaagattc agccatcgac tcaggaggag atcttgttga
    13681 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
    13741 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
    13801 agagccagac aataaatcaa aacctgaaat aacccctgga gaaaaaccag atgtaactcc
    13861 agaaccagat acggattctg gaaaccaaac agtaccggaa acaaatccag acacagataa
    13921 tgaaactgaa aatccagaaa aaccagaggt agatccagaa gaaaaaccag atgtgactcc
    13981 agaaccagat acggacgcaa gagaccaagg aataccagaa aaaataaaca aaaaaacaat
    14041 tcaagaggat ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa aaataaatga
    14101 agagcagctg aataaaaaat cacgaatatt tgatagcgcc caatcagctg aaaccttaaa
    14161 aagtagcaag gatacaacct ttgctgtcct gaaacgaaaa ataaacagct accaaaatcc
    14221 ggagagtcac aaaacaaagt tatactgtgg agcggaatca ttctattaag tatagcaaca
    14281 atgctttctg caaaaagatt taaacaaaac agatctctct aacatttgaa taatgtctgt
    14341 gaacgcattt ttcaaataaa aagaaatatc cagaaaatga ccccaaaata ccagattgaa
    14401 atctgatatt tggggtcgtt catactttat ctttcttcat atttatttta agggatatct
    14461 gtttcttttg gaagatggtt cgggatcttt ttcactttca atacatctaa aaagaaggta
    14521 aagatcggaa tgccgacaat caagccccaa acgccaaaga accgttcgct aacaagtaag
    14581 accacaaatg tataaaaaat cggcagttct gttttgctgg acatcaactt cggattcagc
    14641 acataagact caattagatg gacaatcaaa atcgtaatca ggatataaac aacatctcgg
    14701 atgcctcctt gggaataagc tatgaaagat agaggaatac aagaaatgat aacacctgct
    14761 actgggatca gacttaaaat gaaaatcatg atcgctaaac tcagcagctg atgaaagcca
    14821 aatacagcca aggcgattgt agtgagcaac gtatttaaaa gagcgacaac aaattgagct
    14881 tctaatacca aaccaaatgt attgacaaac ttatcggcaa aatagtaaat atcttgaaag
    14941 aaccaagaaa attcactttt taaaaacaat cttgagaata caattgtttt tttcttttca
    15001 atcataaaaa agaaacttaa gatgaatgac atagcaaacg ccactgccat ttttcctata
    15061 tcctgtaaat agccaagcgc aatcgaagcg ccattttgaa gctgttcaaa aaaatcgttt
    15121 cgctcaatat aagtatggat atattgtaaa acaggatctg catcttttgg ctgatgctcg
    15181 taaaaatcaa taacggactg gatcatatca aatgtttgat gaataagcat cgggatatac
    15241 tttgtgaatg ccagatagat aaggtaaacg atcagtgtat aaatcatgat agctaaaaca
    15301 cctgtaggga ttcggacaaa tcgctgaatg atttgaacaa gatgaatcat caaataagta
    15361 aagataaatg tgagcaaaat ggttgtaatc atacttctaa ctaagaaaag aacaaaaacg
    15421 acaaataata aaacagtgag acggcgtatt ttttcattcg ccaaaaaacg ctgataaacg
    15481 gtcaaatcga caacctccaa ataacaaaaa ataaacacgt tattcattat atgatggaat
    15541 atgaaaataa gaaagagaaa gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag
    15601 ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg gtcttgatga
    15661 aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt
    15721 ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa cgcagcacct
    15781 tgaggagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg acgattaata
    15841 taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc cagacacaaa
    15901 tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa catcacaaga
    15961 tactgggtat aggtaagatt cagcggcttg aggagggaag tgtagtgtct aatgatttcc
    16021 ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc ttccattttt
    16081 ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa gtctattcac
    16141 taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac ggacacactt
    16201 tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg atagatcctt
    16261 caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa acttgtgcac
    16321 tgacagtaga agccccagag atattttttt gctgtttgat caattctaat gcactgttaa
    16381 aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt
    16441 taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgaact tctccagagc
    16501 gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta ccacctttaa
    16561 ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag aaatggattg
    16621 gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat agcaaacata
    16681 tacaaaaaaa tatcagagac aaaaactaat ctcatcataa acattacata tatttctcaa
    16741 aaagttgttt tctttcttca aaacttactt cacgatggac ctgatgaagc atccataaat
    16801 aaccaaaagg atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt
    16861 cttgaatctc tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga
    16921 cgatgatatt gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag
    16981 acgtgttttc atccagcata tggaatctcg tgccatacaa ggtaaaaatg acttcattat
    17041 tgcctctagg gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca
    17101 gttctagtgc tttcaaacta tcatttaata caaaatctat ttccgttcct ttatgcataa
    17161 agatctcctc tccttttcag tcgaacagtt actagattct tttattatac cagttaaaga
    17221 aaacgcattc aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa
    17281 atccgcgtcc cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt
    17341 cttctatcaa tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct
    17401 cttttgactt tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct
    17461 cagcacttaa gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt
    17521 tctttcgatt gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata
    17581 aaatagcata aagaaactct tctattccaa aaacaaacag attaagagaa acaacgaatg
    17641 cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg
    17701 gagggatcgt cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag
    17761 caaaaatcgc acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg
    17821 gaactattgc aatgattaaa ggaaaaaaca agcttccgaa aagtacttta aaaaagggtt
    17881 ttttacctaa aaatagaaga gctagtacca acataacgat attcaacact aaaataacta
    17941 tagctcggtc aaatcctaaa acagcttcca gtaatattcc tagaccgctg acacctccag
    18001 cagcaatatg atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa
    18061 aaataatctt aatgtatgtc catataaatt gtctcgtttc tttcatttat ccacttcctt
    18121 aat
//
LOCUS       DAYYBG010000057        16883 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000057 DAYYBG010000000
VERSION     DAYYBG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.058"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1203)
                     /locus_tag="ACHAUN_001817"
     CDS             complement(31..1203)
                     /locus_tag="ACHAUN_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFM8460509.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            complement(2322..2663)
                     /locus_tag="ACHAUN_001818"
     CDS             complement(2322..2663)
                     /locus_tag="ACHAUN_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8460510.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(2663..2893)
                     /locus_tag="ACHAUN_001819"
     CDS             complement(2663..2893)
                     /locus_tag="ACHAUN_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8460511.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            3180..3518
                     /locus_tag="ACHAUN_001820"
     CDS             3180..3518
                     /locus_tag="ACHAUN_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460512.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(3723..4285)
                     /locus_tag="ACHAUN_001821"
                     /pseudo
     CDS             complement(3723..4285)
                     /locus_tag="ACHAUN_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(5017..5598)
                     /locus_tag="ACHAUN_001822"
     CDS             complement(5017..5598)
                     /locus_tag="ACHAUN_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8460513.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(5781..6407)
                     /locus_tag="ACHAUN_001823"
     CDS             complement(5781..6407)
                     /locus_tag="ACHAUN_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8460514.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(6429..7760)
                     /locus_tag="ACHAUN_001824"
     CDS             complement(6429..7760)
                     /locus_tag="ACHAUN_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8460515.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(7915..8316)
                     /locus_tag="ACHAUN_001825"
     CDS             complement(7915..8316)
                     /locus_tag="ACHAUN_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8460516.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(8402..8608)
                     /locus_tag="ACHAUN_001826"
     CDS             complement(8402..8608)
                     /locus_tag="ACHAUN_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460517.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(8703..10205)
                     /locus_tag="ACHAUN_001827"
     CDS             complement(8703..10205)
                     /locus_tag="ACHAUN_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8460518.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(10223..10678)
                     /gene="argR"
                     /locus_tag="ACHAUN_001828"
     CDS             complement(10223..10678)
                     /gene="argR"
                     /locus_tag="ACHAUN_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8460519.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(10776..11393)
                     /locus_tag="ACHAUN_001829"
     CDS             complement(10776..11393)
                     /locus_tag="ACHAUN_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8460520.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            11614..12987
                     /locus_tag="ACHAUN_001830"
     CDS             11614..12987
                     /locus_tag="ACHAUN_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8460521.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            13062..14174
                     /locus_tag="ACHAUN_001831"
     CDS             13062..14174
                     /locus_tag="ACHAUN_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8460522.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(14246..15883)
                     /locus_tag="ACHAUN_001832"
     CDS             complement(14246..15883)
                     /locus_tag="ACHAUN_001832"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8460523.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(16051..16659)
                     /locus_tag="ACHAUN_001833"
     CDS             complement(16051..16659)
                     /locus_tag="ACHAUN_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8460524.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4686 a         3450 c         2953 g         5794 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc ttttatcttt tacacaatta tatggacttt atcaataacc aacagcaaaa
     1321 aatacaatga aaaacaaact tggaactaat gtatcattaa taaatgttga aaaattcagc
     1381 aactcattac tgtatgattt tagcagccca accaacatat accctataaa agctaataag
     1441 atggttagga atcctttaga atttttactc attcatagac acttccttaa ataactttta
     1501 ttatagaagg tattgtaaat aataatattg acaaaaagtg actcaaaata aaaaaaacct
     1561 tacattatac tggaatgaac tgtattgata gtgtacctaa aagctctact gacataagat
     1621 cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta gagaataaac
     1681 taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac tttggttcac
     1741 tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa
     1801 ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga tgttccagtg
     1861 atggcaaatt tctcttttca ttgtttttct tgtcgcatga tatgcctttt tgtgttattc
     1921 agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta cgtttttgag
     1981 catcgatata gtttaaaatg acattactca tatctaaaga gtttagattt tttaggacta
     2041 aaactgtcag ttgttctttt tcttctttct gttaaagtat tgtcgcgact tttcgtatac
     2101 gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa
     2161 atcacctcaa tcattcgttt aaaatgctat atattcttta gtttaagata cggaaattaa
     2221 tgccatcgac cacactagta gaatgaacga catttacttg gccttttttt aatttcgtta
     2281 tgtttagcat ataaaaatat tatagagttt acttacaatg cttaaaacat atactgtata
     2341 atctgatcaa ttttagccat gtcttgcaat ggtaagtttt ctattttttt gagttttcgt
     2401 tccttaaaat caagagattt taattgtgta ataattactt gtccttttat atctagctct
     2461 tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg aagtaattgg acatatgacc
     2521 gaaaacattg ttttacggtt aaattcataa cgagagacga ctagtcctgg tcgtcttttt
     2581 tgaatttctt ttcctacgga gggatcaaaa tcaatccata caatatcacc ttttttagga
     2641 atataaattc tttcttctga cactataaaa tttctctccc ttcaggagtt aaatcttccc
     2701 attcgtcttt ctcataatac tcggcctctt ccccaccact aaatggatct tcaatttttg
     2761 gaactaacgt aattgttcca tcatcagaat acacaacgat atattcttgg ttttctgaag
     2821 gtttctttcc gttatcagaa ggtaaagtta ccactacaga acttccttgt aatctagatt
     2881 ttgtaacaag cattatcatc actcccttaa taaaagtata ctatataaca ctaagtaacg
     2941 caaaattccc ctttgtaaac tatctagaaa attgttttgt ttaaagtata tcttattgga
     3001 tataaataga aataaattgt agccagttca ttttttcttt aactaaaaaa ataatccaag
     3061 aaacattgaa cctatggatc caaaagctac ccaaatacta tagctaaccc cccgctacct
     3121 tcaattttgc agataaaacg ctttgggaga aaatttcaaa gacaacaggc catcctatca
     3181 tgcccgatta taaacaaaaa gctggctatt ccttttgctc tgacaaattt caattgggaa
     3241 agttcacgaa actagcaggt gaagaagttt caactgttcg gatcaaagaa tgcccgcttc
     3301 aaatagaaac attggttcaa cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg
     3361 agatcgtagc tatttttgta gaagaaggga ttttacatga tccaactcat atcaatgtgg
     3421 ataaatggca acctcttatc tataaattca gagaatatac aactgccaac cagtcgttaa
     3481 ctttgaattt tagattccaa gaattcaaag agtcataatg ataaacatcg ttaggtataa
     3541 ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag ctatacatta gaaaggtcga
     3601 ctgtgaccct atagatgcag tatatgcagc ttatgagcat ttcttctgtt ctctttgttt
     3661 ttgggatctg tgctgaagtc tttttcgttt tttgccgctc tttctttgaa aaatcgatta
     3721 tgttatacct tctccattac taattcaggc atctgctgag taatgatttt tcgcgcaaaa
     3781 gaaatatctg ttacccaagg ttcgatttgt tcttcagtca agacaagcgg cattcggtca
     3841 tggattggtg cgactgtgcg gttaggttca gtagtcatta tgattgattc tgccattttt
     3901 tcatcctgat ggctacgata aaaaccgcca atatacacta ttttttcctt atttgaaaac
     3961 ataaatttgt ctttttcttc attccactca ttgggaaaac acaacgattt tgcaaaaatg
     4021 gttgatgaaa ggtctttttt tcttctactg tctctgcacg ggcattaatc atcaattgtc
     4081 cttttttaaa tccggtaaat ccccacttca tgatttccgg agtgaggatg ttttcttcct
     4141 cttttcttaa cgtcacaaca agatttgacg gaaaaacttc tccagctgct aattctgctg
     4201 cctgcttcgg ttgtttcgtc tgggcttctc gataataatt ctgaaggagc tctgactcta
     4261 gatcaaaaaa ataacgacca cacatcctaa tccctcccaa tctttttttc tatcatacac
     4321 tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt
     4381 gacaagtgac aaactctcca gtgaatggta ttctcctgcc aactcctcgg tcacaagtca
     4441 caatattcaa caaacatgag aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa
     4501 acggcaggct tacgacctct gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc
     4561 caaatttatg caaaaatgta gaaagctatt ttcacaaaat ttttcttaat actcgaaact
     4621 aagcgacttt ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat
     4681 aagcccaacc aaacgaaaaa tatgagcagc tatttttatt tggctgttct tattacttga
     4741 tgcagggcag ctttttcaca acctctccac taaacggtgt tcaatcagct aaccttcggt
     4801 attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc
     4861 aggtcaaagc gctgatttca caacctctaa tacacggtgt tccagaagta gcttcttcga
     4921 aaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
     4981 cttgaagctg accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa
     5041 caatctttct ttgatcaatt gaaaattctg cctaagtagt gatagatcct gtgtgacttt
     5101 caacaataag atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc
     5161 ttcatcggac agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca
     5221 ttgattgact aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat
     5281 aatcaaatta cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat
     5341 ccagtccaac atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc
     5401 gaacaattct gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt
     5461 ttttgaagaa aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc
     5521 tcggattgac gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat
     5581 cttttccttt gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca
     5641 gtaaattcat cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca
     5701 cagccctcac tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga
     5761 ggacaaaagc aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact
     5821 tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg gccaggagca
     5881 ccaattgcat ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg
     5941 gaatcgctac acaagtttcc gttcattcca ccaagaatta ctgtgtcgat tccatttttt
     6001 ctcaattgga gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgatg
     6061 atcgtatttt catccaaata aggtttcaat tcttcgataa aatcggctcc tgaaccttct
     6121 tccactgatt catattgact ttttctagca aacatctgct gctcaagcat catatgttct
     6181 cctgcaccgc caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag
     6241 agtttgtatc ctttttcttt tgcttgtttt accatttcaa tcatattttc gatcgtattg
     6301 tatttttcta agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg
     6361 acttgcgggt cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca
     6421 ttcgttattt attttaatac atcatttaga gcttcactca ttgtcggatg agaatagatt
     6481 tgatctctca ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg
     6541 acttcatgag aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg
     6601 actaaaactt tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt
     6661 gtcatatact tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa
     6721 cctacattgg ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc
     6781 gttgttcgtt tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag
     6841 atataagtga actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct
     6901 gtacgaaggt attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt
     6961 tctaatccaa gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct
     7021 tggattgtta cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct
     7081 gctacttgat ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct
     7141 aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa attcagaatg ggcatcaaga
     7201 acgacaactt tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca
     7261 gccccgatga tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca
     7321 atcaagtagc gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta
     7381 ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt
     7441 tgatggttag aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg
     7501 atcatatgat aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc
     7561 tctgtaaagt cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg
     7621 atgttgatac atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattca
     7681 ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata
     7741 ttttgatatt tttccatcat ttttccctcg cttctttttt acctaccgat tggtacaaaa
     7801 atatgataac cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc
     7861 agacaaaaag cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac
     7921 aatcgttact tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg
     7981 cgcatcttct gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttctaa
     8041 tactacaccg atctgatagc cgccatcatc taaattataa agagagacac gtgcttttac
     8101 tgtggactct gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca
     8161 agaagcataa cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg
     8221 aggagtgatt ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc
     8281 atggttgatc attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca
     8341 tttgctactt ggctataatt atagtgtatg acaagtaagg cgctttctac aaaaattctg
     8401 cttattcgaa taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac
     8461 tcgtgaacaa cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt
     8521 ttatatgatt gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg
     8581 ttaaaaaaat aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc
     8641 actcgattta accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg
     8701 gtttattctg ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt
     8761 actaacgcca acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc
     8821 tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat
     8881 cgaatacctt cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc
     8941 ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat
     9001 cggatttctt gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata
     9061 ccttttcttt ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca
     9121 tccagaaaat agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta
     9181 tctttcccaa gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata
     9241 taacgaactt ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg
     9301 gaattcgtca cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg
     9361 atcaaattat ttcgattgat cactgttcca gaagcactga caactgcaga acgatccaca
     9421 tctgtatcga aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt
     9481 tccatcgctt ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc
     9541 aaaaattggg aacctgttga atccgcacct aagctattca atactttttc agcaaagaat
     9601 ccaccagcac catttccggc atcaacaatg attttccaac catttagtgg tttgctttct
     9661 agtgttccaa tccccttttt aatttttgtg actaaatctt gcgcataagg tgtcaaaagg
     9721 tctgcttttt ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata
     9781 aaagcaatat cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa
     9841 taatagggaa gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat
     9901 tgagtggaca taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct
     9961 tgttctttca gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca
    10021 tgaccaatcc caatcgtcca ttttttattt ggatcggttg atttcttctt tttctttatc
    10081 cagtttgcta gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat
    10141 ccttcaactg gtaatgcgat tcctcgaata tccgatccat tttgcagcgc tttcatatct
    10201 ttcatataaa agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct
    10261 gtgcttcttc ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga
    10321 caacttcagg gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt
    10381 cagtcaatac gataaccatg aactgaacag actggatacg caaaacctcg cgtctaattg
    10441 ctgtacgcaa acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta
    10501 ctattttgtt ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg
    10561 ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc
    10621 caataggttg ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat
    10681 tagacacctc tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta
    10741 ttttttactt ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc
    10801 aaaatcgtct tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg
    10861 tgttctttag aagctctttc atagccttct tttgcgatga atgcttctaa tttctcaaac
    10921 gttgctggtt cttcggcata gggcccgacg tgtaagattt gggcacaaag accttcttcc
    10981 atctttttga atactacttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg
    11041 gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa
    11101 aaatgttctt ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct
    11161 tccagtggat agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac
    11221 atgcgtattg catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga
    11281 ttcgggtcgc cgatccctct gatgctaaag aatgattttt taggaacctc tagtatttgc
    11341 gggctttttt tcatactata gctcttgtct ttacgccatt caattttttc catttttctt
    11401 cctccgcgaa attccattta gttatttatt atcatacctc tttttgagtg acttggctaa
    11461 tcgtgttgac actatttttc agtttgtgct agaataagtg aagtaaataa atcgtacaga
    11521 taacacgttt cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct
    11581 ctttttttac gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta
    11641 gtacctgcag taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc
    11701 atgaatgtaa ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa
    11761 tgggtaacaa cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg
    11821 aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt
    11881 ataatgatcg atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa
    11941 ggtgcagcga caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc
    12001 ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca
    12061 attggcccca cttatggagg cattatgact tcacatttta catggaatca tatcttcctg
    12121 tttcttgtgc ccgttttgtt tctttctttg attatcggct ggtttgctat tcccgagatg
    12181 gcagtacaaa aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt
    12241 agtggaagtc taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc
    12301 atcggcggta ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccactg
    12361 atccgactgt ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt
    12421 tatcagtttt tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa
    12481 ggaagtagtc cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg
    12541 ctttctcctt tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct
    12601 gggctgattc tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca
    12661 gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc
    12721 aatatgatga ccaccggaat gaatgcactg aatgaagaac tacaaggaga cgggaacgca
    12781 atgttcaata ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt
    12841 atcaattacg tccagcatca tacttcccat aactatgaag tgtctactac attaggatca
    12901 aaagcagcat taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat
    12961 tatctgttct ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc atctgccgtt
    13021 tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta
    13081 tcggtggcgg gatcattggt atgaccctag ccagttactt agatacatca aaattcaatg
    13141 ttgctttatt tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc
    13201 cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt
    13261 ttttccctaa acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga
    13321 cacttttgct tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa
    13381 aagaagaagc gccagaaatt ggaaacatcg aatggctttc acctgaacag acatctcaag
    13441 cacttccgtt attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg
    13501 gaaaagcata tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc
    13561 aggcaaaagt cactttgcat aagaagagcg attcttggct catcaaggga ccgtttggag
    13621 aatacacagc tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag
    13681 aattaggcta tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat
    13741 cttataagat gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc
    13801 ctttcatgga tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt
    13861 tggctcctac tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg
    13921 atccagatca gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt
    13981 cagatttttc cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg
    14041 gtctaggctc ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct
    14101 taaatactgg tcattggcct acggaagatt atcaaaagga catctccacc tatattacga
    14161 aaaatccatc atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg
    14221 attacttctt gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt
    14281 cttttgcttc atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa
    14341 tatcaagtcg ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat
    14401 ctggtatagg taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt
    14461 gttgcacgat tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga
    14521 aatatccagt attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag
    14581 cttgatagtc ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta
    14641 gctcatctag ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg
    14701 ttgcaccaaa agacgctgct aaaactgggt cattgatctt gataattggc ggaagcgaat
    14761 catctttcat gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg
    14821 gtgtcgcata acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta
    14881 ggactttttc tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag
    14941 aaatggccgg atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg
    15001 cagtagcaaa accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag
    15061 agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa
    15121 atacagccat cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg
    15181 caataaagcc attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt
    15241 ctccaaagta acgtaatcct agcacagccg ctgtattatg gagagcatca aacaaaatga
    15301 gtccatttgg aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct
    15361 catcaaagga aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat
    15421 tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca
    15481 cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac
    15541 tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct
    15601 caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg
    15661 tcctaacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga
    15721 tcgttcctgg actccctttt gctagttgat aagctgtctt cctatcaaaa acttcatgaa
    15781 cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc
    15841 cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc
    15901 taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc
    15961 tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt
    16021 tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt
    16081 tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg
    16141 attggcagct gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta
    16201 acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc
    16261 agagcttgaa gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc
    16321 aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc
    16381 tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc
    16441 accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat
    16501 tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt
    16561 ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc
    16621 aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc
    16681 gttttttatt ctttcgacgc tattaacttt atcatttaga aatatttcat aggtaaaaga
    16741 aacatgacat ttaatgacaa aataacgaat tatttaatat ttgtaacatt taaacaaata
    16801 tttttttgat acaaaaccat aaaagccttg ttgtataagc aaagtaatcg tttttctatg
    16861 tttttttatt tttgaatgaa aaa
//
LOCUS       DAYYBG010000058        16408 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000058 DAYYBG010000000
VERSION     DAYYBG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.059"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHAUN_001834"
     CDS             complement(<1..1835)
                     /locus_tag="ACHAUN_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8460525.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHAUN_001835"
     CDS             complement(1832..2536)
                     /locus_tag="ACHAUN_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8460526.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHAUN_001836"
     CDS             complement(2548..4857)
                     /locus_tag="ACHAUN_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8460527.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHAUN_001837"
     CDS             complement(5052..5516)
                     /locus_tag="ACHAUN_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460528.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHAUN_001838"
     CDS             complement(5516..6142)
                     /locus_tag="ACHAUN_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8460529.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHAUN_001839"
     CDS             complement(6149..6514)
                     /locus_tag="ACHAUN_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8460530.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHAUN_001840"
     CDS             complement(6504..6842)
                     /locus_tag="ACHAUN_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460531.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHAUN_001841"
     CDS             complement(6832..7158)
                     /locus_tag="ACHAUN_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8460532.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACHAUN_001842"
     CDS             complement(7136..7417)
                     /locus_tag="ACHAUN_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8460533.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACHAUN_001843"
     CDS             complement(7419..8783)
                     /locus_tag="ACHAUN_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8460534.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHAUN_001844"
     CDS             complement(8796..9365)
                     /locus_tag="ACHAUN_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8460535.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHAUN_001845"
     CDS             complement(9337..10524)
                     /locus_tag="ACHAUN_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8460536.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHAUN_001846"
     CDS             complement(10588..12315)
                     /locus_tag="ACHAUN_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8460537.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHAUN_001847"
     CDS             complement(12312..12764)
                     /locus_tag="ACHAUN_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8460538.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHAUN_001848"
     CDS             complement(12876..13256)
                     /locus_tag="ACHAUN_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8460539.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHAUN_001849"
     CDS             complement(13253..13639)
                     /locus_tag="ACHAUN_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8460540.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHAUN_001850"
     CDS             complement(13641..13919)
                     /locus_tag="ACHAUN_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460541.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACHAUN_001851"
     CDS             13967..14695
                     /locus_tag="ACHAUN_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8460542.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACHAUN_001852"
     CDS             complement(14841..15317)
                     /locus_tag="ACHAUN_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8460543.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15611..15796)
                     /locus_tag="ACHAUN_001853"
     CDS             complement(15611..15796)
                     /locus_tag="ACHAUN_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460544.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACHAUN_001854"
     CDS             complement(15797..16069)
                     /locus_tag="ACHAUN_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8460545.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACHAUN_001855"
     CDS             complement(16066..16266)
                     /locus_tag="ACHAUN_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460546.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT         4605 a         3463 c         2547 g         5793 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaat
//
LOCUS       DAYYBG010000059        16079 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000059 DAYYBG010000000
VERSION     DAYYBG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.060"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACHAUN_001856"
     CDS             226..894
                     /locus_tag="ACHAUN_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8460547.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACHAUN_001857"
     CDS             complement(1094..1414)
                     /locus_tag="ACHAUN_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460548.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACHAUN_001858"
     CDS             complement(1451..2833)
                     /locus_tag="ACHAUN_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8460549.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACHAUN_001859"
     CDS             complement(2989..4176)
                     /locus_tag="ACHAUN_001859"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8460550.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACHAUN_001860"
     CDS             complement(4183..5214)
                     /locus_tag="ACHAUN_001860"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8460551.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACHAUN_001861"
     CDS             complement(5230..6684)
                     /locus_tag="ACHAUN_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8460552.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACHAUN_001862"
     CDS             6859..7764
                     /locus_tag="ACHAUN_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8460553.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACHAUN_001863"
     CDS             7855..8532
                     /locus_tag="ACHAUN_001863"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8460554.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACHAUN_001864"
     CDS             8529..8699
                     /locus_tag="ACHAUN_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460555.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACHAUN_001865"
     CDS             8813..9709
                     /locus_tag="ACHAUN_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8460556.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACHAUN_001866"
     CDS             complement(9764..11134)
                     /locus_tag="ACHAUN_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8460557.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACHAUN_001867"
     CDS             11372..12118
                     /locus_tag="ACHAUN_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8460558.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACHAUN_001868"
     CDS             12115..12954
                     /locus_tag="ACHAUN_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8460559.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACHAUN_001869"
     CDS             12955..13905
                     /locus_tag="ACHAUN_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8460560.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACHAUN_001870"
     CDS             14046..14798
                     /locus_tag="ACHAUN_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460561.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACHAUN_001871"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACHAUN_001871"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8460562.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5217 a         3003 c         3232 g         4627 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaattt
//
LOCUS       DAYYBG010000060        15743 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000060 DAYYBG010000000
VERSION     DAYYBG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.061"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACHAUN_001872"
     CDS             complement(24..722)
                     /locus_tag="ACHAUN_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8460563.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACHAUN_001873"
     CDS             complement(715..2067)
                     /locus_tag="ACHAUN_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8460564.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACHAUN_001874"
     CDS             complement(2290..3690)
                     /locus_tag="ACHAUN_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8460565.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACHAUN_001875"
     CDS             3851..4588
                     /locus_tag="ACHAUN_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8460566.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACHAUN_001876"
     CDS             4713..5192
                     /locus_tag="ACHAUN_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8460567.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACHAUN_001877"
     CDS             5189..5956
                     /locus_tag="ACHAUN_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8460568.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACHAUN_001878"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACHAUN_001878"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8460569.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACHAUN_001879"
     CDS             complement(6859..7461)
                     /locus_tag="ACHAUN_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8460570.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACHAUN_001880"
     CDS             7567..7797
                     /locus_tag="ACHAUN_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460571.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACHAUN_001881"
     CDS             7788..8036
                     /locus_tag="ACHAUN_001881"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8460572.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACHAUN_001882"
     CDS             8142..8726
                     /locus_tag="ACHAUN_001882"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8460573.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACHAUN_001883"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACHAUN_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8460574.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACHAUN_001884"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACHAUN_001884"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8460575.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACHAUN_001885"
     CDS             10627..10806
                     /locus_tag="ACHAUN_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460576.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACHAUN_001886"
     CDS             10828..11853
                     /locus_tag="ACHAUN_001886"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8460577.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACHAUN_001887"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACHAUN_001887"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8460578.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACHAUN_001888"
     CDS             13425..14372
                     /locus_tag="ACHAUN_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8460579.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACHAUN_001889"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACHAUN_001889"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8460580.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACHAUN_001890"
     CDS             complement(15274..15516)
                     /locus_tag="ACHAUN_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460581.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACHAUN_001891"
     tRNA            15637..15709
                     /locus_tag="ACHAUN_001891"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5350 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tta
//
LOCUS       DAYYBG010000061        15250 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000061 DAYYBG010000000
VERSION     DAYYBG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.062"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACHAUN_001892"
     CDS             2..1021
                     /locus_tag="ACHAUN_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8460582.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACHAUN_001893"
     CDS             1018..1785
                     /locus_tag="ACHAUN_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8460583.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACHAUN_001894"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACHAUN_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8460584.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACHAUN_001895"
     CDS             3750..4070
                     /locus_tag="ACHAUN_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8460585.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACHAUN_001896"
     CDS             4070..4420
                     /locus_tag="ACHAUN_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8460586.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACHAUN_001897"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACHAUN_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8460587.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACHAUN_001898"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACHAUN_001898"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8460588.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACHAUN_001899"
     CDS             6414..7439
                     /locus_tag="ACHAUN_001899"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8460589.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACHAUN_001900"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACHAUN_001900"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8460590.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACHAUN_001901"
     CDS             8517..8957
                     /locus_tag="ACHAUN_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8460591.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACHAUN_001902"
     CDS             8960..9562
                     /locus_tag="ACHAUN_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8460592.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACHAUN_001903"
     CDS             complement(9625..10728)
                     /locus_tag="ACHAUN_001903"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8460593.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACHAUN_001904"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACHAUN_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8460594.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACHAUN_001905"
     CDS             12294..12689
                     /locus_tag="ACHAUN_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460595.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACHAUN_001906"
     CDS             12689..12940
                     /locus_tag="ACHAUN_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460596.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACHAUN_001907"
     CDS             12983..13378
                     /locus_tag="ACHAUN_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460597.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACHAUN_001908"
     CDS             13375..14631
                     /locus_tag="ACHAUN_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFM8460598.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACHAUN_001909"
     CDS             14643..14966
                     /locus_tag="ACHAUN_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460599.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACHAUN_001910"
     CDS             14959..15225
                     /locus_tag="ACHAUN_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460600.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5238 a         2580 c         3225 g         4207 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg
//
LOCUS       DAYYBG010000062        15067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000062 DAYYBG010000000
VERSION     DAYYBG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.063"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACHAUN_001911"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACHAUN_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACHAUN_001912"
     CDS             706..1734
                     /locus_tag="ACHAUN_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8460601.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACHAUN_001913"
     CDS             1731..2489
                     /locus_tag="ACHAUN_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8460602.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACHAUN_001914"
     CDS             complement(2575..3315)
                     /locus_tag="ACHAUN_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8460603.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACHAUN_001915"
     CDS             3376..4629
                     /locus_tag="ACHAUN_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8460604.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACHAUN_001916"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACHAUN_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACHAUN_001917"
     CDS             5300..5626
                     /locus_tag="ACHAUN_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8460605.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACHAUN_001918"
     CDS             5694..6407
                     /locus_tag="ACHAUN_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8460606.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACHAUN_001919"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACHAUN_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACHAUN_001920"
     CDS             7690..9555
                     /locus_tag="ACHAUN_001920"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8460607.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACHAUN_001921"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACHAUN_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACHAUN_001922"
     CDS             11120..11821
                     /locus_tag="ACHAUN_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8460608.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACHAUN_001923"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACHAUN_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACHAUN_001924"
     CDS             complement(12857..13066)
                     /locus_tag="ACHAUN_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8460609.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13307..13882
                     /locus_tag="ACHAUN_001925"
     CDS             13307..13882
                     /locus_tag="ACHAUN_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8460610.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="ACHAUN_001926"
     CDS             13882..14322
                     /locus_tag="ACHAUN_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8460611.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="ACHAUN_001927"
     CDS             14338..15039
                     /locus_tag="ACHAUN_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8460612.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5080 a         2501 c         3179 g         4307 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaa
//
LOCUS       DAYYBG010000063        15035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000063 DAYYBG010000000
VERSION     DAYYBG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.064"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..157)
                     /locus_tag="ACHAUN_001928"
     CDS             complement(<1..157)
                     /locus_tag="ACHAUN_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8460613.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFK"
     gene            complement(163..393)
                     /locus_tag="ACHAUN_001929"
     CDS             complement(163..393)
                     /locus_tag="ACHAUN_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460614.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(829..1395)
                     /locus_tag="ACHAUN_001930"
     CDS             complement(829..1395)
                     /locus_tag="ACHAUN_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8460615.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1395..2351)
                     /locus_tag="ACHAUN_001931"
     CDS             complement(1395..2351)
                     /locus_tag="ACHAUN_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8460616.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2351..3007)
                     /locus_tag="ACHAUN_001932"
     CDS             complement(2351..3007)
                     /locus_tag="ACHAUN_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8460617.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3377..5395
                     /locus_tag="ACHAUN_001933"
     CDS             3377..5395
                     /locus_tag="ACHAUN_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8460618.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5465..5671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5822..8236
                     /gene="leuS"
                     /locus_tag="ACHAUN_001934"
     CDS             5822..8236
                     /gene="leuS"
                     /locus_tag="ACHAUN_001934"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8460619.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8584..10137
                     /locus_tag="ACHAUN_001935"
     CDS             8584..10137
                     /locus_tag="ACHAUN_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8460620.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10518..12302)
                     /locus_tag="ACHAUN_001936"
     CDS             complement(10518..12302)
                     /locus_tag="ACHAUN_001936"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460621.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12317..14038)
                     /locus_tag="ACHAUN_001937"
     CDS             complement(12317..14038)
                     /locus_tag="ACHAUN_001937"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460622.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            14138..14899
                     /locus_tag="ACHAUN_001938"
     CDS             14138..14899
                     /locus_tag="ACHAUN_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8460623.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4885 a         2776 c         2950 g         4424 t
ORIGIN      
        1 ttttaaagta gttcttatta gaatagtaga gattaattaa taagctatct tcgattgcta
       61 aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta ttcgaaggat
      121 caaatttaaa ccataattgc gtctcattaa aaagcataac cattactccg atctattttt
      181 aaacttagtc tctacctcta atatatatcg agtttttatt ttgccttcag agaatactgt
      241 ttcttttttt gcagttacag gttgtttatt ttcggaaaaa gctaatatag ctaaaattga
      301 aacatccatc tggaatttat cttttttact gctttgatca taaaagtctg catattcatc
      361 actgatattt tttctaataa attcttccat cataaaaatc acctcaaaac gattatacga
      421 acttacgttc ttgttgtaaa gcgatatttg aggggcaaaa aaggggcata atttgactga
      481 tattaagtta gctattgata tatgaatgac atacaaaaat ataaaatctt ttaaaaatgc
      541 ttatatatca atgtttttgc cattctttta tatatctgtt atatgtcaaa aaaatgtgtt
      601 caatttcaat ttgtatttga ataaagaggt tgtgaaaaag ctgtcctgca tcaagtaata
      661 agaacagcca atcaaaaaca gcttctcatg tttttgattt ggttggactt attaccgcag
      721 atataagctt ttgagcaccg tgaataaaga ggttatgaaa tcaacgctct aacctgagta
      781 ttagagaaaa aaacgcaaaa catatcattt atgttttgcg ttttttattt atcttttttt
      841 ctcaatacaa tataaaatag gcggctggtt cttttggttg ataaaccgat aattcagcac
      901 attatatgtt tcctgtggca gtttggaaca aaaagaatca accatatcca gttctttttc
      961 tccaccttca tgaccatagt agacgacaag gatcaatcgt ccgcgaggca caagtcgttt
     1021 caatatttct tccatagctg tgcgtgtagt ctctggcata gtgatgattg ctttatcact
     1081 ttttgggaga taccctagat tgaaaatccc tgctttgaga ttttgttgtt cagctatcac
     1141 ttctcctaac gtttcgtggc cttgtagaaa caatgagaca cgttcttcta actgacgctg
     1201 ttctagcctt tcacgagtag catcgattgc ttgttgctgg atatcaaaac tatagacatg
     1261 accagttttt ccgatgtgtt ctgctagaaa aaccgtatcg tggccattgc ccatcgttgc
     1321 atctacgacg tgatcccctg gttgcaagat ttcttgcagt aatgtatggc tgaaatgcaa
     1381 agcagtttgc aacatcagac gctcaccttt ttccgtacat catattttcc ttggaagcta
     1441 tctctgcgaa gaagttcttc atcaattgca ttcaagactt cccatttttt caagctccac
     1501 attggtccaa tcaaggaatc ccacggcgca tcccctgtca aacgatggat gatgatctct
     1561 tttggtatca tttccagctg gtcgcaaatg acattgacat agtcaggacg gctcattaat
     1621 tgaaggcgac cttcatgata gtcccgcaac attctcgttt tgcgcatcag atgcatcaaa
     1681 tggagcttga ttccttggat atctgaatct aaaatggtgc gtctgacatt ttctttcatc
     1741 atttctaaac tttcgcctgg taatccgttg atcaaatgcg tacaaacacg gatattatgt
     1801 ttgcgtaatt tagccacgcc atctaaataa gtttgataat catgtgctcg gttgatcagt
     1861 tttgaagttt cttcaaaagt tgtttgcaat ccaagttcta cccataaata catccgctcg
     1921 ttcagttcag caaggtaatc aactacttca tctggcagac aatccggtcg tgtaccgact
     1981 gataatccta cgacacctgg taaattgact acttgctcaa atcgttcttt gattatttca
     2041 actggcgcat gggtgtttgt aaaattttgg aaataaacga tgtattgttg gacatgaggc
     2101 catttttgat gcatcatgtc gatttctttc tggaattgga ttggaagagg gtcttcaggt
     2161 gcaacgatca tgtctcccga accagataca ctgcaaaaag tacaaccgcc atgggcaaca
     2221 gtcccatctc gattcggaca gtcaaatcct ccatcgatcg gcaccttgaa aattttcccg
     2281 ccaaactcat ttcgtaaagc gtaattccaa gaatgatatc ttttatttcc ttctgaataa
     2341 ggaaaagtca ttaaaagtcc ttctttctgt aagagttatc tatttgtttt cttttttctc
     2401 tatacactgg ataagtcagg cacagccaag aaagactgag taagtaaccg ccgatgatat
     2461 cagtaggaaa atgaacgccc agataaatgc ggcttatccc gacacttact ataataaaca
     2521 tcatcagcaa ttgaagcaat gtgcgtaagg ttcttttttg aacaaaagct ggaagcagaa
     2581 atagtaaaga accgtaaaga atcatactgc ctgctgcatg tccactaggg aaactgtaac
     2641 ttgtttcaac tactaagtgc gccaatgaag gacgctctcg catgaaaaat agtttgatca
     2701 ccgggttgat gattccagca attacaataa atccagaaac gaaccaaaat gtttctgccc
     2761 tttttttctt ataccacatc cataaagccg cacaagcaaa taaaatgatg acggtaagag
     2821 gattaccgaa tttagtcata aagagttggt agctgttcag atcaggataa gaagctctga
     2881 caatgcctgt aaaccactga tcgaaaccag aaagccacga cagatggaac tttacgatcc
     2941 agcccaaaat gaaaaacagg acaagaaaga agctaccagc cacctgaaat gatgttttat
     3001 atttcataca attcctcctt tgaagcaatg acttattata gcatttttgg tccagcgaaa
     3061 aaaacggtaa agagataaaa ccaatgtatg attcctcttt gaataaccaa tattcttata
     3121 aaaaaaataa ctgtaatcgg aaagacattt tttggtaatg caaatgaaat gttagggaaa
     3181 taaaatcagt gtttttcgaa atccttttaa acaagaacgt agacgtagga agaacttgaa
     3241 aatgaacaga agttgttcat tacggtaaat tacaatatga ttacaaaaaa caacaaagct
     3301 cttaaataac ttttaataaa tattaagaga agttataatt attaataata gataaattga
     3361 agttggagga tagaagatgg aaaacattgc aagaaaagaa cggagacgtt tagaacagac
     3421 caaacgtttt cgaaaagcaa aacgaggggc agcaatcgtg ggaactgcga tggtaggctg
     3481 ttctgtcgct gccccattag tacagccggt tcctgttcaa gcggatgaaa cacctgttca
     3541 gttcggcgca agaatcaata cagcggcatt tatcacagaa attgctactt atgctcaacc
     3601 aatcgctcaa gccaacgatc tatatgcatc agtgatgatc gcacaagcag ttgtagaaag
     3661 cggttggggc agtagtgcgt tatctcaagc accttattat aatctttttg ggataaaagg
     3721 cagctatcaa ggacaaaccg tctacatgga tacgcttgaa tttttaaata atcaatgggt
     3781 aaataaaaag gagcctttcc gacaatatcc ttcttttgct gaatctttca atgacaatgc
     3841 ctatgtactt agaaatactt catttggcaa cggttattac tatgccggta cttggaagag
     3901 caacacgaaa tcctatacag atgcgacagc ttgtttgaca gggcgttatg cgactgatcc
     3961 tggttacgca ggcaaattaa ataatatcat cgcaacttac ggattgaccc aatatgatac
     4021 accagctagc ggaaacgcag gcggtggagc gactacaggt ggcaataata ctggaagtgc
     4081 aggtagtact ggcggttcaa gcaattctgg taatacaaac tcaggttcag caacttcgac
     4141 cacttatact gtccaatctg gtgattctgt ttggggaatc tcaaataaat tcggtatcac
     4201 aatggatcaa ttgatccaat ggaacaacat ccaaaacaat ttcatttatc caggacaaaa
     4261 actgacaatc aaaggtagcc aggaaaatgg ctcttcgacg aataattccg gtaacaatac
     4321 caattcttca ggaaatgctg gtacatcaaa cggaggacaa acaactggtg cgaaatacac
     4381 tgttcaatct ggtgattctg tttggaagat ctcaaatgat cacggaatta cgatggctca
     4441 attgattgaa tggaacaata tcaagaacaa tttcgtttat ccaggacaac agctaatcgt
     4501 atcaaatggc ggttcttcta atgctggagc aaatactggc tcaaatactg ggaatacaag
     4561 tggctctagt tcgtccagct caagttcttc taatacagct tcaggaacga aatatacagt
     4621 taaagccgga gactctgttt ggagcgtctc aaataaatac ggaattacga tggctcagct
     4681 aatccaatgg aacaatatcc aaaataactt tatctaccca ggacaacaac tgactgtttc
     4741 aaacagtgga tcaacaaaca gcacgtcagg taatacaggt tcgtcaacca atacaaataa
     4801 cagcagttca gttaatactg gcagcagttc gtctcaaggc acttataccg tgaaagcagg
     4861 tgaatctgtt tggagcgtgg caaataaaaa cggcatcacg atggatcagt taatcgaatg
     4921 gaataatatc aaaaacaact tcatttatcc aggacagcaa ttgatcgtta aaggtggaac
     4981 ttcggcaaat acaaatacag gttcaactac ttctgcagcg aaaccaaata caccgaatac
     5041 gtctgctacg acaagtacat catccactgg taatacgatg tataccgtga aagcgggcga
     5101 gtcagtttgg agcgtagcga acaaacatca tatcacgatg gatcagttaa tcgaatggaa
     5161 taatatcaaa aataatttca tctatccagg acagcaagtg attgtgaaga aaggttcatc
     5221 tcaatcaaca aaccaacaat cagttactgg ttcgaaaacc tatactgtga aatctggaga
     5281 atctgtttgg agtgttgctg acagtcatgg aattacaatg gctcaactaa tcgaatggaa
     5341 caatattaag aacaacttta tttatccagg tcaaacgttg attgtagcta aataaaatag
     5401 ctgttcgaag aaataacgat tgaattccac aaaaatattt gttactataa ggctacttaa
     5461 atatgacaac acttgaaaag gaagtagtaa aaagactgaa gtttcagaga gcgcatggct
     5521 ggtgagaatg tgcacggatg cttttgaact cgcctttgag ttgcccttgc tgaatacccg
     5581 agtaagcaag cggcggtgac ggccgttaca acgttcgagg ccttttcact aaggtaaaat
     5641 taggtggtac cgcgtagatt acgtcctata aaaagagcaa tctttttata ggattttttt
     5701 atttcttttg tgaagtttga gaaaacaaag caaaaggaaa taaaaataaa cagattctgc
     5761 cggttttttc gttatattta taatagaaac aagtgttttc aatagaacag gaggacagca
     5821 aatgagttac aatcataaag aaattgagaa aaaatggcaa aaatactggg caaaaacgaa
     5881 tactttcaac acccatgatg atcctgaaaa gccaaaattt tacgcactgg atatgtttcc
     5941 ctatccatcc ggtcaagggc tacatgtagg gcatcctgaa ggttatacgg caacggatat
     6001 cctttcacgt gtgaaaagaa gtcaaggata caacgtgctg catccaatgg ggtgggatgc
     6061 attcggtctg cctgctgaac aatatgcgct agatacagga aacgatccag ctgagtttac
     6121 ccaaaaaaat atcgaaacat tccgacgcca aatcaattct ttaggattta gttatgactg
     6181 gaatcgcgaa gtgaatacaa ctgatccgga gtattataaa tggacacaat ggatctttac
     6241 gaagctttat gaaaaaggtc tagcttatga agcagaagta gcggtaaact gggtacctga
     6301 attaggaaca gttatttcaa atgaagaagt gatcgatggg aaaagtgaac gtggtggcta
     6361 tgatgtagtc agaaaaccaa tgcgccaatg gatgctgaaa atcactgctt atgctgatcg
     6421 cttattggat gatctagaat tagttgattg gccagaaagc attaaagaaa tgcaaagaaa
     6481 ttggatcggt cgttcagtag gagcaaatat tgaatttaaa gtggctggta cggataaatc
     6541 ctataccgta tttacgacac gaccagatac tctattcggt gcaacttact cggttcttgc
     6601 acctgaacta gatcttgtta gagaaattac gacccctgaa caaaaagctg ctgtagaagc
     6661 ttatatcgaa gagacagcta aaaaatcaga tctaaagaga acggatcttg caaaagaaaa
     6721 aacaggagta tttactggtg cttatgcaat caatccagtt aatggcaagg aaatcccaat
     6781 ctggatcgcc gattatgtat tatcttcata cggcacagga gccatcatgg ctgttccagc
     6841 acatgatgaa cgtgactttg aattcgctca aactttcgga ctagaaattc ttccagttat
     6901 cgaaggtgga gatgttgaaa aagctgctta cacagaagat ggtacgcaca tcaactctga
     6961 atttctagac ggcatgaaca aacaagaagc aatcgataaa atgaatgaat ggctagaaga
     7021 aaatggcgta gggaaaaaag aagtcagcta tcgcttacgt gactggctat tctcccgtca
     7081 acgttactgg ggtgaaccaa tcccaatcat ccattgggag gacggaacag tcacagctgt
     7141 cccagaagaa gagctaccat tacggttgcc taagacaaaa aacatcaagc caagcggtac
     7201 tggagaatct ccattagcaa atatcgagga gtgggtcaat gtcgttgatc ctgtaacagg
     7261 taaaaaaggc cgtcgcgaaa cgaatacaat gccacaatgg gcaggaagct catggtatta
     7321 cctgcgttat atcgaccctc ataacaaaaa agaactagca gattatgaaa agttgaagcg
     7381 ttggctacca gttgacatct acattggtgg agcagaacat gcggtacttc atttactata
     7441 tgcacgattc tggcataaat tcttatacga tatcggtgtt gttcctacaa aagaaccatt
     7501 ccaaaaacta tacaatcaag gaatgatctt aggcgaaaac aacgaaaaaa tgtcaaaatc
     7561 tcgtggtaat gtagtcaatc ctgatgatgt ggtcgaaact tacggtgcag atacactgcg
     7621 catgtacgaa atgttcatgg ggccattaga tgcttcgatt gcttggagcg aaaatggctt
     7681 agaaggaagc cgtaaattct tggatcgtgt atggcgtttg atcgttgatg agaacaataa
     7741 aatgcgtgac cgtatcacta cattgaatga tggcaagctt gataaagtgt atcatcaaac
     7801 agtgaaaaaa gtgacagaag actatgaaaa tcttcatttc aatacagcaa tctcccaatt
     7861 gatggtcttt atcaatgaag cctataaagt ggacgctctg ccttatgaat atatcgaagg
     7921 atttgttcaa ctactcgcac cgattgctcc tcacatgggt gaagaactat gggcaatcct
     7981 tggaaatgat ggtggtattt cttacgcacc atggccaaca tacgatgaag cagcacttgt
     8041 tgaagacgaa gtagaagttg ttttccaagt aaacggtaaa gttcgtgcga aaagcaatgt
     8101 tccccgagac ttaggaaaag atgagctaga aaaagcagca ttagccaatg aaatagtgca
     8161 agaatatatt gaaggcaaaa cagtcagaaa agttattgcg gtaccaaaca agttagtcaa
     8221 tatcgttgct aattaattgg ctctttgtca ataaggactg atgagttgtg caaaatcaaa
     8281 tctgggccag aatgaacttc attctgtctc agattttttg ttatttcaaa ttgattaatt
     8341 gattgatcaa aaaggttttg attttcatgt tctcaaacac gttcaatgtc tttttcttct
     8401 gaatggtctt gtaagagatc tctgtaatca tcaaattttt gtctattaat cttagtattt
     8461 tttgatgtat aaactacctg aaattgagag tacataccaa attcataaat tttttattag
     8521 tgggactagt gattgtatgg attttgacgc ataatgaatg aagtatgaga aaaggagaga
     8581 caaatggatc aagaactatt tattatcgaa gtattatcag ctaaacaaaa aattatcgag
     8641 aaagcgaaaa aaagagcgaa aagaataatg ttcaagcttt taagcttagt atttttctct
     8701 ggtttgcttt ttttcggagc tgtatcgttc ctatatcgcc atatgagtgc agtagtccgt
     8761 tcagctgaag ggatgcaaaa attaaataat ttgtatcact tttcgtgggg agttggcgga
     8821 ataacaattg ttgtgcttat tgtttttctt ctacttattt ggctccgtta ccatcaattg
     8881 tcgcgtcatt tcgatcaatg gcttagtcaa agagcacaac agtttttaca taataaacca
     8941 acttctcaag atgatgacta tctttatttt gagaagactt cgacgagtca agcttttcgg
     9001 atacaaaaaa gtgattgcca gttattgctg agatcacttg atgatttacc tatctactta
     9061 ggcgaaagca aatcaaaatt cggaatgaac acattccata tttttttact agaaaacgaa
     9121 cccagatggg aaaaaagcca gtcttcgcct ggtggatacc tgacaactaa gatgagtaca
     9181 ttagtcactg gttttttcct cattctttta gctggaacaa tctattatgg gagatacact
     9241 cagccaaaaa tgtcttctca tataaatgct gcttctcaca tgacaatgaa ctcaactaac
     9301 gatacgcata tacaaaagac aaatgaaggg ttgttagctg atcctaagca aaaggtaaat
     9361 ttgacaacac aaaagataaa tgatctcaaa cttgatacag agacgcactc tctctatatg
     9421 acaacagata gtggtgctaa ttgggtattt gtgccgattg attcgcaatg gttgcgtttt
     9481 ggcgaataca ccctgacaac ggggctgatt cctgaaggtt attggatgga caacacatat
     9541 gatatttccg ccgatttctc atggtttatc tattcttctg gggatgatgt acgtctgctg
     9601 acttcaagag ataatggtaa aacatggcaa acgtctatta tcacccaatc agttcagccg
     9661 atacgttatc gaaaagcggt attttatggc aacgatggtc tatttatctg tacatcagcc
     9721 agtgggatgt ctgcagaaac tttgtacatt tacactacta tggatggcgg aatcacttgg
     9781 ataaaaagtg gaagtacaat gatcaatcag ccagtacaaa atgtcagctt cgttacaact
     9841 tctttaggtt ttgtttcgac acgtgaaagt ttgttttata caaacaacgg aggacgctca
     9901 ttcaaagaat cagttctgtc gattccggaa aactatcaga caggggggat agatcttttt
     9961 caatcaccaa atgaagttac tcaaataagc gcgaatacgt tagagacaaa attttatttg
    10021 ttgaaaaaag gaccaatcga tgcagggaaa atgtttgcct gcctcttccg ttcaactgat
    10081 aacggggaaa catggcaatt cgttcagcaa ctatcgcaag tagaacaagg agattaaaga
    10141 agagagattg tgagcctgac gttcagcacc gagtcacaag aaacaaattc aaaaaacagc
    10201 ttctcatatt tgttgaatag tgtaactcgt gattcggagt tgacaagagc aacaagaagc
    10261 gattttgaag tatagcttct cgtattttac aagatcgtgt cttattgccg ataggttgtc
    10321 tcttgaacac cgtttattcg taaaaaggga ttgtgacaag tcttttgtca ctacttaccg
    10381 aacgaaactg aaggagctat ccaaaaagta tcattagtat tattctttgt gtttctagtg
    10441 gcaccactag tacatttaaa aaagctatcg atcatggact aatcatacat gatcgatagc
    10501 tttttagata atgtttgtta cgctatctga aattgactat tataaagact ggcataaagc
    10561 gttggtgatt ggagtagttc agaatgtgtt cctttttcga cgatatcgcc attcttcatt
    10621 acaaggatca tgtctgcatt ttcaatcgtt gacaaacggt gtgcaatcac aaaacttgtc
    10681 ctatcttttg tcacagcttc cattgcttgc tgaatcaatg cttctgttcg tgtatctacg
    10741 ctagaagttg cttcatctaa aataacgaca ggaggattcg ctagaatgac tcgagcaatt
    10801 gtcaataatt gttgctgtcc ttgagataac gtgccgtttt cgctagaaat gatcgtatca
    10861 tatccgtctg gcagtgttcg aataaagtga tcacattgtg cgatttcagc cgcttcgata
    10921 atttgttctc ttgtggcatc cattttcccg taggcgatat tatcagctac cgttccttca
    10981 aacagccagg tattttgtaa aaccatccca aaagaacgac gtagttctcc acgtgaaagc
    11041 ttggtaatat caagaccgtc gaattcgatc tttcctttat tcagttcata aaaacgcatc
    11101 agcagattga ctaaagtcgt ttttcctgca ccagttggac caacgatcgc taccgtttgt
    11161 tttggcttta ctgaaaaatc gacttgattc atcaatattt tatctgatgt gtaaccaaac
    11221 tgtacatttt tgaaagctat ttttcctttc ggttgttcta aaagtcttgc atctggaagg
    11281 tctggaattt catctgtttc atctaatatc tcaaatatcc tatcgattgc tgccattgcg
    11341 gattggatcg agttgatcac ataagaagcg gtagaaattg gttcagaaat ctgattgatg
    11401 tactgaagat aagcttgtaa gaagccgatc gtcacaccac ctgatagaac aagcatagca
    11461 ccgaataccg cacttatgat gaaggcgagt tgattaatga aacgaatggc cggatagata
    11521 gcacagttca taaattgagc acgtttaaaa gcgttgtatt gctttttatt tacttcgtgt
    11581 atagcagctt ctgcatgagc ttgctgattg aatgttttga ccactagatt accagctaag
    11641 atttcttcca ttcgattatt taaaacaccc agttcatact gattctgttc agaaatctgt
    11701 ttgtttttgt tcgcaatctt agttgtaaca attgaactgc caacgatcag caataagacg
    11761 agcaaagtaa gcttgctttc gatataaaac agcatcagac cagcagcagc tattgttacg
    11821 acagaagtaa aaaattgatt gatacctgtc aataacactt gagaaagttg attgagaccg
    11881 gtagttgtcc gactaatgat gtcacctact tgatgattgt caaaaaacga catcggtaat
    11941 gttttgaatt tttttgtgat ttcttttcgc accctcaatg tgatcttttc gcttaacgct
    12001 gccattgtcc gttcctgaat aaatgaagtg atgctgctta agactgaaca gccgatcagc
    12061 aataagacag ggaacaacaa tgcttgtttg atatcactga aagaaagctg tccaatacct
    12121 gtttttctta tttctgataa gagatcatcg atcccgattc ccatgatcaa gggcatggct
    12181 acgactaata ggttcccgaa taaactgcat agaaacaatc cgtaaaaata tttatgttca
    12241 ggtttgatca ttttccaaaa acgtatcaaa gcacgcgtgt tcaacgtttt tttcttttcc
    12301 atcgtatccc ttctttctat attccttgtg attttgcaaa ctcctgataa gtttggcaat
    12361 tagacagtag ttcttcatga gtaccttgtc caacgatttt tccttcatct aaaacaatga
    12421 tgttatctgc ttgttggatt gtactgaggc gttgagcaac aatcacgaga gttttctccg
    12481 acatgtgttt atgaagggct gctcttaatg ctgcatctgt tttatagtct aaagcggaga
    12541 aactatcatc gaatacatag atctctgctg gtttgatcaa cgctcgagca atacatatcc
    12601 gctgtttctg gccccctgaa taattagaac cgccttgagc aacaaagctg tccaatccgt
    12661 caggaagggt gtccactagt tcttctaatt gagcgatctt gatcgcttta gacaactctt
    12721 ctgttgaggc atattcattt cccatctgca agttgcttaa aattgtcccg ctgaataaga
    12781 aagccttctg agggacataa ctgatgtggg agcggatctg ttcttgagtc atttgagtaa
    12841 gaggaatacc accaagggtt acttttccta tcgtagggtc actaagtctc aagagaagtt
    12901 tcgccaaact actttttcca gatccggtcc cgccaacgat cgctgttgtt tttcctttag
    12961 gtattgtgaa atcaatatct tctagaactg gatcagagat ttcagtatat ttaaatgtga
    13021 catgttcaaa agcagctgca tttgtctgat caaaggttgg ttcagctact gttttgatcg
    13081 tctcttcctc aatttcgatc tctgcttgca gtatttcttc tatccgtcgt aatgaggcaa
    13141 acgatttagg gataatgacc aataccatag ctgcgatcat caagtaagca agtgtcagca
    13201 tagagtattc gactacagca gtcacgacac cgatttgtaa tgctccttct gctacaagat
    13261 ttccgccaaa ccacaaaata gaagcataaa cgattcccat gagcaaaaaa gcaattggag
    13321 tgacccaagc aaatattcgg ttcactttga tcatttgatt agcatagtca gcaaagctct
    13381 tattcgttcg cttttcttca tatgattgat tatcaaaggc acggatcata ttaatcccag
    13441 taaagaattc tctcaatgtg atggtgatcc gatccatttt tggctggata ctcaaagata
    13501 aaggtgcccc tcgtttcatc agtcgataaa caatccatac ataaaaaaca acagagatca
    13561 atgtaaccca tgttagtaat agagaataac gaaacatcat gagaatggtg aaaacgcaaa
    13621 taaccggtgc tggtaagatc atttggcaaa ataaaacaat catttgctgg acattatcga
    13681 catcattcgt cattcttgtc aaaagagatc cagaactaaa acgactgata tccttcattg
    13741 acagctgttg gaatttacgg aaatatttct cccgcgtttg gaatccaaac ctagcggaag
    13801 ccaatgctga aaagtaacta gaggcaactg cagcgattgt tcctagaaac gctacaagca
    13861 gcatttcaac tccaattttt tttataactg aggcttcgcc acttgctata cctgtgtcga
    13921 tcagctttgc tactaatttt ggtacaccta atgtaccggc gatttctaaa cagatcatca
    13981 caaaggtaag gagagcgagt cctttgtttt tctgtaagaa tgtttttaag atagacatat
    14041 tattcctcct tttttcctga tacttttcga ttctatacct tcaagtaact tgaaggtcaa
    14101 tagaaaaaag aataaactga aaaagaggtg agaaaaaatg aaaaaaatgt atacgattgg
    14161 ccaagtatcg gaactatttg atttgccaaa atcaacgatc cgctattggg atgaacaagg
    14221 attaatccgt tcaagtagac aagaagagaa tgattatcga ttgtttgata tcgatgatat
    14281 ttttatgctt tatgatatcg ttttttatag aaaattagat ataccgatca aacagatgaa
    14341 gaatttatat ggaaaaacat tgacggagtt atatgagaca cttgatgaaa cagaaagaag
    14401 aatccataaa gaactggtgg tgatgaaaca aaaacaaaaa gagataaggg agcaaaaaaa
    14461 acagttgaaa ttgatgatcg atacaaacga ggaagaattt cctgtagaag agatcccttt
    14521 tgactgtatg atctcaacgg agtttgaaga tattgtggaa atcaaaaaat ttctacctaa
    14581 ctattcaagc tttgggatga tgagtatgcc agcttccagt tcacagacga tgtatggatt
    14641 ttttatcgac cctagtgaag tacatttatt tactcaagac gtgatttggg agaaaaaaga
    14701 tacggctgtt tatcgtcgat tcttattgaa atccgagatg aaccatgcgg aacgtaataa
    14761 tattttagaa atcagagaaa gaatgtatga aaaaggatgg aaaacaggag aagtgatcgg
    14821 ccagtatcta ttgacgaata cggatgaaaa caatatcaga actgaatatt atcatgcatg
    14881 gatagaaatg aaaaaatagg aaatatgtgt ttaagataac tcgaaaatga tggttgtttt
    14941 agttataaag aaaaattttc tggttcgatt acgggtttaa tgtgggggat tattagtcgt
    15001 ggatagatag aattttagtt ttttagagga aatat
//
LOCUS       DAYYBG010000064        14624 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000064 DAYYBG010000000
VERSION     DAYYBG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.065"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..815
                     /locus_tag="ACHAUN_001939"
     CDS             3..815
                     /locus_tag="ACHAUN_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8460624.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="ACHAUN_001940"
     CDS             812..1612
                     /locus_tag="ACHAUN_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8460625.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="ACHAUN_001941"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="ACHAUN_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8460626.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="ACHAUN_001942"
     CDS             3109..4818
                     /locus_tag="ACHAUN_001942"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8460627.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9388
                     /locus_tag="ACHAUN_001943"
     CDS             5030..9388
                     /locus_tag="ACHAUN_001943"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8460628.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9533..10006
                     /gene="rimP"
                     /locus_tag="ACHAUN_001944"
     CDS             9533..10006
                     /gene="rimP"
                     /locus_tag="ACHAUN_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8460629.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10029..11204
                     /gene="nusA"
                     /locus_tag="ACHAUN_001945"
     CDS             10029..11204
                     /gene="nusA"
                     /locus_tag="ACHAUN_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8460630.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11226..11519
                     /locus_tag="ACHAUN_001946"
     CDS             11226..11519
                     /locus_tag="ACHAUN_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8460631.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11516..11827
                     /locus_tag="ACHAUN_001947"
     CDS             11516..11827
                     /locus_tag="ACHAUN_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8460632.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11840..14146
                     /gene="infB"
                     /locus_tag="ACHAUN_001948"
     CDS             11840..14146
                     /gene="infB"
                     /locus_tag="ACHAUN_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8460633.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14173..14520
                     /gene="rbfA"
                     /locus_tag="ACHAUN_001949"
     CDS             14173..14520
                     /gene="rbfA"
                     /locus_tag="ACHAUN_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8460634.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4982 a         2598 c         3255 g         3789 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5701 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5761 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5821 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     5881 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     5941 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6001 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6061 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6121 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6181 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6241 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6301 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6361 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6421 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6481 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6541 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6601 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6661 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6721 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6781 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6841 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     6901 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     6961 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7021 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7081 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7141 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7201 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7261 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7321 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7381 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7441 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7501 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7561 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7621 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7681 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7741 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7801 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7861 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     7921 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     7981 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8041 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8101 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8161 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8221 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8281 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8341 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8401 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8461 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8521 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8581 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8641 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8701 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8761 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8821 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     8881 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     8941 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9001 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9061 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9121 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9181 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9241 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9301 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9361 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9421 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9481 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9541 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9601 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9661 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9721 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9781 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9841 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
     9901 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
     9961 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10021 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10081 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10141 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10201 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10261 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10321 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10381 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10441 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10501 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10561 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10621 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10681 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10741 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10801 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10861 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    10921 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    10981 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11041 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11101 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11161 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11221 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11281 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11341 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11401 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11461 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11521 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11581 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11641 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11701 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11761 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11821 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    11881 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    11941 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12001 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12061 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12121 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12181 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12241 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12301 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12361 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12421 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12481 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12541 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12601 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12661 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12721 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12781 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12841 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    12901 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    12961 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13021 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13081 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13141 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13201 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13261 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13321 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13381 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13441 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13501 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13561 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13621 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13681 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13741 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13801 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13861 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    13921 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    13981 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14041 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14101 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14161 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14221 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14281 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14341 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14401 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14461 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14521 tcaaaaactc ccgcaaatcc tgtgaaatca ggctctttgt caataaggac tgatgagttg
    14581 tgcaaaatca aatctgagtc agaatgaacc acattctggc tcag
//
LOCUS       DAYYBG010000065        13808 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000065 DAYYBG010000000
VERSION     DAYYBG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.066"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHAUN_001950"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHAUN_001950"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8460635.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACHAUN_001951"
     CDS             complement(2261..2512)
                     /locus_tag="ACHAUN_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8460636.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACHAUN_001952"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACHAUN_001952"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8460637.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACHAUN_001953"
     CDS             3462..3680
                     /locus_tag="ACHAUN_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460638.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="ACHAUN_001954"
     CDS             complement(3969..4481)
                     /locus_tag="ACHAUN_001954"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8460639.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACHAUN_001955"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACHAUN_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8460640.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="ACHAUN_001956"
     CDS             complement(6934..7377)
                     /locus_tag="ACHAUN_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8460641.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="ACHAUN_001957"
     CDS             complement(7378..8166)
                     /locus_tag="ACHAUN_001957"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8460642.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACHAUN_001958"
     CDS             complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACHAUN_001958"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8460643.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="ACHAUN_001959"
     CDS             complement(9281..9868)
                     /locus_tag="ACHAUN_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8460644.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACHAUN_001960"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACHAUN_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8460645.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="ACHAUN_001961"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="ACHAUN_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8460646.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="ACHAUN_001962"
     CDS             complement(12785..13720)
                     /locus_tag="ACHAUN_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8460647.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4469 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DAYYBG010000066        13374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000066 DAYYBG010000000
VERSION     DAYYBG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.067"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..575)
                     /locus_tag="ACHAUN_001963"
     CDS             complement(<1..575)
                     /locus_tag="ACHAUN_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460648.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKG"
     gene            complement(586..1254)
                     /locus_tag="ACHAUN_001964"
     CDS             complement(586..1254)
                     /locus_tag="ACHAUN_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFM8460649.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1385..1969)
                     /locus_tag="ACHAUN_001965"
     CDS             complement(1385..1969)
                     /locus_tag="ACHAUN_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8460650.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2304..2534)
                     /locus_tag="ACHAUN_001966"
     CDS             complement(2304..2534)
                     /locus_tag="ACHAUN_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8460651.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(2549..3499)
                     /locus_tag="ACHAUN_001967"
     CDS             complement(2549..3499)
                     /locus_tag="ACHAUN_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8460652.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3496..4338)
                     /locus_tag="ACHAUN_001968"
     CDS             complement(3496..4338)
                     /locus_tag="ACHAUN_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8460653.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(4471..5196)
                     /locus_tag="ACHAUN_001969"
     CDS             complement(4471..5196)
                     /locus_tag="ACHAUN_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8460654.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(5657..5971)
                     /locus_tag="ACHAUN_001970"
     CDS             complement(5657..5971)
                     /locus_tag="ACHAUN_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8460655.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(5987..7582)
                     /locus_tag="ACHAUN_001971"
     CDS             complement(5987..7582)
                     /locus_tag="ACHAUN_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFM8460656.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(7605..8495)
                     /gene="iolE"
                     /locus_tag="ACHAUN_001972"
     CDS             complement(7605..8495)
                     /gene="iolE"
                     /locus_tag="ACHAUN_001972"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFM8460657.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(8512..9537)
                     /gene="iolG"
                     /locus_tag="ACHAUN_001973"
     CDS             complement(8512..9537)
                     /gene="iolG"
                     /locus_tag="ACHAUN_001973"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFM8460658.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(9561..10568)
                     /locus_tag="ACHAUN_001974"
     CDS             complement(9561..10568)
                     /locus_tag="ACHAUN_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8460659.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(10681..11823)
                     /locus_tag="ACHAUN_001975"
     CDS             complement(10681..11823)
                     /locus_tag="ACHAUN_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFM8460660.1"
                     /translation="MPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTGHLQKIK
                     ELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTAKVVAVL
                     AANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKMMMKDLV
                     FMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEAMKKILN
                     TLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPHGISNAM
                     LLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLSIPTIRD
                     YGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(11917..13371)
                     /locus_tag="ACHAUN_001976"
     CDS             complement(11917..13371)
                     /locus_tag="ACHAUN_001976"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFM8460661.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         4000 a         2892 c         2193 g         4289 t
ORIGIN      
        1 aatcctttat tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc tttgaagtcc
       61 aaatttgaag acggttcatc caatataatt aatttagagt ttagcgataa actacatcca
      121 atagaaattt tctgccgttc accacttgat agcgaaaaaa tgtttttatc taatatattt
      181 gaaagatcta ataattcagc taattcattg attcttttat ccatatgctc ttttgtaaat
      241 ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa gaattgtcct
      301 ctaggatctt gacttacgta gccaatattt tttgatatac tttcttgtgt taaggattgt
      361 agcgagttat tttcaaataa aatttcccca accaattttc cgtcataaag tcttggaata
      421 agtccattca ttaactttaa tagtgtggat ttcccactcc cagaagaacc agtaatcaat
      481 gtaagactac ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc
      541 ttatatgaaa aagaaacatt gttaaattca aacaattctt tcacctcaaa tcaaaatact
      601 taagaaaaaa atagtaacgg aaaacaggat gatatagtct aataagctca tgtttgtttt
      661 atagtaagaa cttcttttca caggagattc tatccccttt gttattgcat tagcggtcat
      721 ttcttcagca atcatagttg cacgtataag aacgggaact aacagaaatt ccaaatattg
      781 cagaggctga aaaaaagaca agcgaatgtt tctcattttt aaagattctt taatcattct
      841 tatttcagaa cgataagtcg gaaagaatct gatcactact gtaatactta acattatttt
      901 tttcggtaaa tgtatttttt ctaaagagga taataattca ttgctagtat agacactaac
      961 caacaaccat gagatcatta cgaccggcaa cattcgaaat acaatatata aggatgtccc
     1021 aaaaaatata gtaaaaaact ctatcttgat agcatcaata attttcatta ctaaaaacag
     1081 aactgaataa atcaagatga aataaataca tgtctttatt ttacgaaaca tcaatactaa
     1141 aactgtagca ataatgaaac accccacttg aaatatattt ccctttccta aaaaaataat
     1201 gaccggaaaa aaaatattta aagtaatcaa ggttaacgga ttgatttttg acactctttc
     1261 acctccaaaa aataagatcg cactgttatc ttaagctgat aaaaatgcac cgttattcag
     1321 ataacagtgc gatcttatca aaaagcataa ctgagaacaa ttctcaatgc aagctttttt
     1381 ttaattaact cagtaattca atccacccag gaacagaaaa ttgatacaat cttctggtta
     1441 atagcatagt cttttctttt gaatagctat gcttcaatat atcatagaat gtagcgtaga
     1501 atgagtaaat gtagaagtgg atttcttctt cattaaagac taaattttga ttgatcattc
     1561 cgatagtctt taacttatct agacaacaga ctgtcttttt tgtcacaaat gatgtaattt
     1621 tttcaacaat atcttgatat ttggttccag ctgattggaa taaaaccaaa tcgaatatac
     1681 gtttattctc aaaaatataa ttaataaatg gatctaattc attgaagttc tctctccaac
     1741 ttttctctat atctatattt tctaagttta agaggtattt ggtaaactct tcattataca
     1801 tattaataag cgcattaata tgcggctgaa taatttcttc aaataacgct tccttagaag
     1861 taaaatgttt ataaaaagca cccgtagtta cgcctgcggt caagcacagt tgccttatct
     1921 tagtttgctg aaatcctact ttttcaaaat ggcttagtcc actttctatt aatttttggt
     1981 gagttgtaat ataatctctg gtttctatgg gtacttcctc ctaataagat gaattaacta
     2041 tattatctta cacaggactt gaagtttcaa tcgttaacgt ctattactga actcattcct
     2101 tagagctaaa ctaatgagaa agagcaaaat aactgataca aaaagcaatc ctacaagaat
     2161 taatagactc atctaaatca ttcacctttt ctaaaataat ccgagaaaga tttaaaaaaa
     2221 ttatatccag ctattaggat aagtcctatc ataatcactc caatagccat tttcttatcc
     2281 tccctaaaaa taaagccttt tacttaaaac ctttttttgt aatcgtcaat aaattttttc
     2341 cctttatcag ttaattgaaa tcctatatct aaatagtcct catatctaag tttagtcagc
     2401 atctcactta cagatgctgg actaacactt agaacttttg ctaaatcatt attttttaat
     2461 ttcttttttc tttttgataa ttcctgaatt gctagtaaat aaattagtat agtagttgtt
     2521 ttaatttgta ccatacctac cctccaaatt atttcgaaag gccttcagca attttatcca
     2581 ggttccattt catcatcgca tagtaactat caccatcttg tcctttttct gcaattgaat
     2641 cagtaaaaat cgttgaatag ataggaatat tggtatcttt tgatactgtt ttcattggtc
     2701 tatcgtccac actgctttct acgaataagg agggaacttt tgttgtgcgt aatttttcaa
     2761 ctaagtgttt tatttgatct ggtgttcctt cttcttcagt gttgatttcc caaatgtaag
     2821 cagaaggcac attatacgct ttcgagaaat atttaaagca tccttcactt gtaacaatca
     2881 tttttttatc attcggaatt gaagcaaatt tagatttagc ttctttgtct agagaatcca
     2941 atttttcaat atacttatct agattttctt tatagaattt tttattatca ggatcttttt
     3001 ccgctaactg tttttcaata tttttagcgt aaataatacc gttttctaaa tttaaccaag
     3061 catggggatc ttccttccct ttctcactct gaccctctaa gtaaataaca tctatgccat
     3121 cacttgctgc aaaatagtct ttgttttcct ctttgttcgc attttttact aattttgtaa
     3181 accaagcatt tcctccagtt tccaagttaa caccgttata aaaaatcaaa tctgcttttg
     3241 aagttttttg aacatcttca ggcaaaggtt catattcgtg gggatctttc ccaataggta
     3301 cgatactgtg taaatcaatt ttatcttttg ctatattttc agtaatatct gctaaaatcg
     3361 aattagtaac tactaccttt aatttttcgt tcgaagccac tgtatctttg tctttactat
     3421 ttgttgaaca tgccccgaat ataaaaagag caatcaaaat ccccagactt cccagtactg
     3481 ctttccattt tctcatcata actttctctc cttctttctt aaaaattttt gttttggtga
     3541 gattagaaaa ctaattacaa ataagaatgc cgctgttaaa acaatacttg atcctgctgc
     3601 tatgttaaaa ctataaccaa tgaacaaacc tagcaatgat gagcacgctc cgactgtcgc
     3661 cgaaataaat atcatagttt ttaaactgtt tgcatataaa tatgctgtag cagctggtgt
     3721 aattaacatt gcaacaatta atatagttcc cacactttgc atagctgtta ctgctactaa
     3781 agttaataga atcattaata gataatgata aaaactgaca ttcatcccca ttgcttttgc
     3841 cattaatgga tcaaaagaag tgattaataa ttctttaaaa aatagaacaa ctgtaaccaa
     3901 cactaaaaca gaaacgccaa ttgtaatcca cttatcaata tcttgtacag ctaaaatatt
     3961 tccaaataag atatgaaata aatccgttga gctatttgca actcctataa ggataactcc
     4021 taatgctaaa aaagaactga aagtaatacc aattgctgta tctcctttta ttacactatt
     4081 gctttttata aaagtaataa tgatagaagc taatagacca aatacaatcg caccaataaa
     4141 aaaattaata cctaaaatat aagaaattgc tactcctggc aaaacagcat gagaaatagc
     4201 atcacccata agagacattc ctcttaaaat aataaaacaa cctactgttc cagaaacaat
     4261 ccctataact acagatgtaa ttagagcatt ctgtaaaaag tgaaagtcat acagaccatt
     4321 aataaaattt tgaagcatat tattcccctt gtatattaga gttagggttt taaccttccg
     4381 gttttgattg gataacccta tgaaccatcg ttattttaat ttggtattaa ctcatttctt
     4441 ttgagataat atatttattg gattgagagg ctaagccgtt cccccctctc caataaatat
     4501 agtatctcca taggcttttt ttaaattctc ttcattaaat acagattcta cacttccgtg
     4561 agcaattaga tttcgattca aaagaataat attatcaaaa tacttcttta ccttgcttaa
     4621 atcatgatgc acaattagca ctgtttttcc ctgatgtttc aattctttta acgtatccat
     4681 aattattcgt tcactaacta agtctatccc tacaaatggt tcatctaaaa atatgaaatc
     4741 cgcattttga gcaagacatc ttgctaaaag tacacgttga aattgtcctc cagataattc
     4801 tcctatttga tgattagaat attttcccat atcgaccttt tccaaagcct ttgaaactct
     4861 ttgccattct gcatttttaa ttctttgaaa aactttcatt ccggcatatg ttcccataga
     4921 tacacattct tttactttta ttggaaaagt aaaatcaata tcagcttttt gctctacata
     4981 tgcaatcttc accagttttt tattgatatc ttcattatta atggagacct ctccctgatg
     5041 agggataata tttaacattg ctttaagcaa tgtagatttt ccagcaccat ttggaccaat
     5101 aatacctgtt aaagcacctt cttgtatttt taaagaaatc tcgttcaatg ctaaaaaatc
     5161 gttataacaa acattaagct ttttcacttc taacataact ttctccttct aaatgataat
     5221 gattatcacg atcatatggt atacaaaaat tatattttag tcaaccctaa attttataat
     5281 ttagattttt ttctgttgtt gaagattagg atgtcaaaat tatttgaaac tactcgtata
     5341 gtaaatagta agtaattttc aaaacacgaa gaaaaacaaa aagaaaaaag gaaacgccga
     5401 agcatttcct ttcagtgatt taatgagaac aacacgattt gacttagagt ctcaaaaagt
     5461 cgaataggta cactcattta agtgtctcca ttcgactttt tggggatgag ctggctattg
     5521 aggtccgctt accctattga ataaatacaa gattgggtat ttctaataag tccgcccttc
     5581 atattggggt gatcggggcg aacatgccat cttttcatat accacctcct cgacactcaa
     5641 taatcaaaga ttctcgttaa tcttcttcga tgcttgcaat gatgttttca atattcatat
     5701 aacgaaaatc ttcttgtttg atataaacga tctgagcggt acatttttcc tttttgatga
     5761 tttcttttac tttttcctca gaacgtgcga cctgcggcgt taaaagtaaa acgtcatagt
     5821 ctttataatt cagtttttct tcaacgtttc ggatcgttgc ataatctatc tcatacgata
     5881 gatttttggc tctgatcgtt tcaagcattt ttgatacgag cagcgacgca ctgattccgg
     5941 tctcgcaaat aatcaataga tccttatgca taataccctc ctgtttttag cctgcaaagc
     6001 caattggtga aaatgccaca taagttaaaa taacaatgac taccaatgct atagcagtca
     6061 ccttggtgta tttccaaggc tcgatatcga cctttgtatc gaatgagttc aacacaaatt
     6121 ctccatcagg tttcaatttg gaaaccacca tcaaaatgat aatgtccaga acgaataatg
     6181 ccgtcaaaac ataaaggaaa tggatatttt ggaaaagcat ttttgatagt gcatacaaga
     6241 cgatatgcac ggcaatcgtc gtttttgccc caaaagcagt tgcttttttt gaataaaagg
     6301 caaagagaat gatcacgagc agcggcatgt tgtatagtcc attgaattct tgaacgaact
     6361 gatacaatcc agccggtgca aaagaaatga atggcgctac gataatggag ataatcccca
     6421 caacgaccgt tactgtttta ccagcacgag cgatttgctt atcatttgct ttcttattga
     6481 taagcggttt ataaaaatcc agtgaaaaca aggtcgatgt cgcgttcaaa gctcccgcaa
     6541 atgatgataa gatcgcgcca aagataacag cggcgaaaat accaaagatc attttaggca
     6601 atacttcact gactaaaaaa ggatacgcgt tatctggatt tgacattaaa ctatcgccaa
     6661 aaatattgcg agcgacgatc cctggaaaga caaggaataa cgcacctaag attttgaaga
     6721 cgccgactag gaaagcgccc ttttgtgcct cagctaaatt cttgccggac aatgcttttt
     6781 ggacgatcat ttgattggta caccagaaat acagattgtt aaacagcatc cctgtaaata
     6841 gtgtcggcca tggaacaaca gccgagtcga tcgatcccac agagttaagc agccatggcg
     6901 tgtttaccac aacgtgttcg atcccgccaa caaagctgcc gtcaccaagc ttcattaagc
     6961 ctaaaattgg gaccagcaga ccacaagcca gcagaccgac tccataaatg gagtcactat
     7021 tggcacttaa cgacaagccg ccgatcagca gatagaggat gccgacaata ccaattgcca
     7081 tcgctacaat tatgatcgcg acgatcggct ggacgcccag cagctcatca atatggaaga
     7141 tcttattgaa caccagcgat ccagaataaa gaaccacggg taagaaagaa gtcatatagg
     7201 tcacaataaa taagatcgaa ataattcgtt tggtaaccgt atcataacga atctcgatga
     7261 aatctgagat tgtattgatg ccgtatttaa aatattttgg caagaatatc acagccaaag
     7321 ctacgatcgc aatcgtagca gttacttccc aggccattac ttccatccct gcatgataac
     7381 tttgaccatt ttgtccgact atttgttcgg tggacaggtt cgtcatgatg atcgtgccgg
     7441 cgattggcaa agcagttaag ctccgccctc ccatgaagaa cccttctgat ccagaagtat
     7501 cgacacttct ggatctagaa taggcaaata accatacaca gatgacaatc actaggaaac
     7561 tgattataga aaaaagattc atcgtctagc tccttttcaa tagattaaag accggtgacc
     7621 tctcgaatgt agcttctggc tttgactgca tattcaaatg gattggcttt agccgggtcc
     7681 tgttctgctt caactacgat ccagccttca tagtcgctgg catggattgc tgtccagatt
     7741 ggagtgaaat caatcatccc atcaccagga accgtgaaga cgcctttctt cacgccgttt
     7801 aagaagctgt ctttttcctt ttctacttct tcagccgttt cttgacgaat atctttgaaa
     7861 tggatgtgtt tgatacgatc ttgatgacgg ttgtaaaccg cgattggatt ttcaccagaa
     7921 aagaccaaat gtcccgtatc gaacaataat ttaactttgt ctggatcagt catttccatt
     7981 aaacgatcga tttcttccgt cgtttgaacg ccggtaccca tgtgatgatg gtaaacgatc
     8041 tccatatcct tttcgtgggc caaatcccct aatttttcta atccttccgc cagttttttc
     8101 cactcttcat cagtgaaaac tggtttatca tcaaagactg gcgctgtcat aatcccttgg
     8161 atactatgac cctgctcagc tactacgatg actttagcgc ccatcgcatg taagaaatcc
     8221 cgatgtgctt tgaaggcttc ggcagtttct tcaaaaggtt tagttgtcag aaacgcactg
     8281 aaccatgcac tggcaacctt taagccgcga atattcaaat attgttccaa cacctctgga
     8341 tcttttggat atttgttgcc aatctctgtt ccggtgaacc cagcaagggc catttcactg
     8401 atacattgtt caaaggtatt ttcactacca agctcgggca tatcatcatt ggtccacgcg
     8461 attggtgcga tggctaattg aatttgtttg gtcatattct cacactcctg cttatttttt
     8521 tagttgtact aaactcttcg tatcgaatga ttcttgacaa gccaacgcga tcttagttga
     8581 ttgcaatcca tcttctgcat taaccgctgg ttgacgtttt tcttgaatac atgagacaaa
     8641 ttcttgggct tcacgaatga aggcttctct gaatcgttct gggaagtttt gtgaacatgg
     8701 acggatgatc ccttgatcgt tcatgatcgt cacaagattt ttctcaggcg cttgcgctac
     8761 ccgcagcatt cctttggttc ccatgatctc tgtttcgaca tgatagccat gggctgcgtt
     8821 tctgccggca actagtaaag ctattgtttt atcgctcagg ctcatcattg cggctccagt
     8881 ttcaagctca ccagcctgat ccaattctgg atacgccgca tttttcccaa tcgcccacac
     8941 ttgatcgact tcttggccgg taaaccagcg aaccaaatca atatcatgga tcgccatatc
     9001 taagaacaag ccgccgctgt cgttattcat ggcaaatttg acaaagcttt ccaagccttc
     9061 gctcggatcg atcccataac aacgaatcag tgtgatttcg ccgatttcgc catcttctac
     9121 cattttcttc gcatactgat acgactcatc atagcggcgc atgaagccta acatgaagac
     9181 ctgttcagga tgctcttcga tgactttttg ggtcttttta atatcttcaa cttcaaggcc
     9241 taatggtttt tcagtaaaga catgcttgcc attttccatc gccaagcgga cttggtcaca
     9301 gtgaaagcct gatggtgaaa caataaagac tgcatcgatc acggaacttt tgaccatttc
     9361 ttcatagctg gaaaaagtat gcttgacgcc taatttcttc tgtgcgaatg ctaattctgt
     9421 ttcaactaaa ctgcacgcag ccgtaagctc acaacaactg acacctgttg ctaaattttg
     9481 tgcatgtact tttcctaatc ttcctaaacc gacgatgcct actctaatgt tgttcatgaa
     9541 atacctccgt tttctgagaa ttactcattg tatagtgctg gtcgttcatc catttgaatc
     9601 attacttttg tttttgtttc acgagactca tgacaagcca tcatggtgat cgctgctaca
     9661 tacccatccc aagcggtagg accgacaacc gcatcatttt tgatcgaatc gatccacagc
     9721 ttgaattcat aatcataggc atccaaaaag cgggtctgcc aatctggtga aacaggtgtg
     9781 taattttttt cttccgattt aattcgtgta taggctgggt ccgtcattcc tacttctgcc
     9841 gtttcgaaaa ccacatcaca tttaatatcg tagccaaagc ggcaattcac aaagatttcc
     9901 aaatcaatcg ttacaccgga ttttgtttga aagatcagca gctgcggatc atgcaaatcg
     9961 gcatgggagt tgctggtttt tttcggcaag atcatttgaa cagattcata atcttcattt
    10021 agcaaccagc gaagcacatc aatctcatga atcgcggtgt tgctgatcgc catcggtgtt
    10081 gtgtagtttt catctgcgct aggatttcta tgggcacaat gcatcattaa tgcttctccg
    10141 tattgcttcg attcgattgc ttctttcagt tcaatgtagc cgcggtcata gcggcgcatg
    10201 aagccaacct gaaccaattt cttcccataa gcaacttccg catcaacgat ccggcggcag
    10261 cctgctgcat ccatcgctaa tggcttttcg cagaaaacat atttcccatg cttgattgct
    10321 tctaacacat actgctcatg ggtcgggtcc caagaagtca caacaacgac atccacatct
    10381 tcagaagcga tcaatgcttc tccggtttca aagaatttag catcgacacg tttggcaacc
    10441 tctttagcag cttttacatt gatatctgag actgctgtca cataagcccc ttgtgttttc
    10501 tgattgatcc tgtcgatatg ctcgatgccg attgcacctg ttccgataac gcctactttt
    10561 actctcaatt acttatcccc ctgttctgtt gtttaagcac acatagtatg cgcttacaaa
    10621 attattcctg tgtagctttt attcttaaaa ggtagagccc tctctttgct ccgcctttta
    10681 ctacccataa gcttgtacgt ataattcctt aatttgacta tgactaggca cctttttatt
    10741 attttgcgga cttccgctgg tcaacgcatc cgcggccatt ttgtctaagg ctgccataaa
    10801 cgcttgctga tcaataccat aatctcgaat cgttgggatc gacagctgct cacagagctc
    10861 ttggattttt ttaatcagat aatccgcagc ctcttccagc gataaatctt cggctgccgg
    10921 ataaataaat ctggcgatcg tcgctaactc agtctgaata gcatcctttg agaattcaaa
    10981 aaagaccggc aataacatcg catttgatat cccatgagga atgtgaaaaa gtgcccccac
    11041 cggtcgactc attccatgaa tcaatgtgac tgacgcattc gaaaaggcaa tccccgcctc
    11101 cgttgaagct aagctcattt gttctctggc ttcagtgtcg tcgctatttt catatgcctg
    11161 agaaagcgtg ttcaaaattt tcttcatcgc ttccagtgaa aatagttgcg ttacttcatt
    11221 ggctttgact gaaacataag attccaaagc gtgacatagc gcatcgactc ccgttgccgc
    11281 ggttactttt ggtggtacag ttttcgtaaa atcagagtca acgattgaga tctgtggcat
    11341 gaaaacgaga tccttcatca tcattttgac tgctgttttt gtattggtaa taactgccac
    11401 atcggttact tccgaaccag ttccagcagt tgttgacact gcaatcagcg gaatgcccgc
    11461 ttgcagctga tcaaaatttc ttgcatgaat cgcttcgaga cttaattgat tagcagccaa
    11521 aacggctacc accttggctg tatccagaca gctgccgcca ccaaaagcaa tgatatgatc
    11581 acattgatgc ttctgaaaaa gcttcagcgc atcataaaca ttttcttgcg tcggttcggc
    11641 ttgaatgtct gtatactccg ctaattgatc atctgttttt cccaacagct ccttgatctt
    11701 ttgaagatgc ccggtctgtt ccataaccgt atcactaatg attaacggct tgttgcctag
    11761 ttgttctgat tcttccataa gaaattggat tgttccgccg ccaattctta aattggcagg
    11821 cattttcagt tgaatattca cagaaaaaac ctctctctgg cttatagcct ataacagaat
    11881 cctcgaagct ctttaacaga gcttcgaagc aaatctttaa atctcacttt gcgcataacg
    11941 tgctgtaaca actttccgat gggtataaaa ctcaacactg tctttgccat tggcatgcaa
    12001 atcaccaaag aaagagtgct tccagcctga aaaggcaaac caagccatcg gtgcaggcac
    12061 cccaagattg acccccagca tcccagcatc aacattttct ctgaagtagc gaatcgctga
    12121 tgcgttgttg gtatacagac acgaaccatt cgcaaattca gattcattcg tcagctcaac
    12181 ggctgccatc aaatctttta cccgcacgat tgatgcgacc ggggcaaaaa tctcatcctg
    12241 ccagatcttc atatccatcg tgacattatc aaaaatcgtt ggtccaacaa aatagccttt
    12301 ttctggaatg ttttcgcgac catctagtac cagtttcgct ccttcatcga cccctttttg
    12361 gatatagtca aaggtccgct gctgtgcatc ttttcgaatc actggtccta aaaagacccc
    12421 ttcgtccaag ccattgccga tcttgatcgc ttttgccgca gccttaaatt tttcaacaaa
    12481 ctgatccgcg atttcctctt ccaaaacaat gacggaggct gccatgcagc gttcaccagc
    12541 agaaccaaaa gctccgccaa taaggtcctt gactgcccga tcaatattgg catctttcag
    12601 cacaacaaca tgatttttcg ctccagtcaa cgcttgtacc cgttttagat tttttgttcc
    12661 ttcacggtaa acgtattcac cgacattttt tgatcctaca aagctgatgc cttttaccag
    12721 tggatcttgc agcaatgtgt tgacaacatc gtgggcacca tagacaacat taaaaacacc
    12781 gctaggaaag cctgcttttt caaccagctc aacaattttc tccatcaaca atggtgtttt
    12841 ttctgaaggc ttcagcacca ccgtatttcc tgccgccaga gccatcggga agatccagaa
    12901 gggcaccatc attggaaaat taaacggtgt gatccctccg attactccga ttgggtaacg
    12961 ataattcgtg atctcaatat cagtcgccgc tgttgataaa gaatctccca tcattaaatt
    13021 ggtgattccc gctgcgtgct ccacattttc gattcctcgt tgaacttctc ccagtgactc
    13081 ggacaatgat ttcccatttt ccagcgtgat caaacgcgcc aattcacttt tatgatctgt
    13141 taaaagctga tgcagcttga acagataacg tccgcgaaca ggaacaggaa cttttttcca
    13201 agtttgaaac gcagcatcgg cagctttaac tgcttcggct gtctctgctt tgctagacaa
    13261 cggtacattc gccagcgctt cttctgtagc cggattcatt acttcttcaa actctgttga
    13321 gcgactagcg acccaacgac catcaatata atttcttaaa aatcttgtca ttta
//
LOCUS       DAYYBG010000067        13363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000067 DAYYBG010000000
VERSION     DAYYBG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.068"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..2323)
                     /gene="glyS"
                     /locus_tag="ACHAUN_001977"
     CDS             complement(248..2323)
                     /gene="glyS"
                     /locus_tag="ACHAUN_001977"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8460662.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2325..3242)
                     /gene="glyQ"
                     /locus_tag="ACHAUN_001978"
     CDS             complement(2325..3242)
                     /gene="glyQ"
                     /locus_tag="ACHAUN_001978"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8460663.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3628..4446)
                     /gene="recO"
                     /locus_tag="ACHAUN_001979"
     CDS             complement(3628..4446)
                     /gene="recO"
                     /locus_tag="ACHAUN_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8460664.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4589..5488)
                     /gene="era"
                     /locus_tag="ACHAUN_001980"
     CDS             complement(4589..5488)
                     /gene="era"
                     /locus_tag="ACHAUN_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8460665.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5503..5898)
                     /locus_tag="ACHAUN_001981"
     CDS             complement(5503..5898)
                     /locus_tag="ACHAUN_001981"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8460666.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5882..6358)
                     /gene="ybeY"
                     /locus_tag="ACHAUN_001982"
     CDS             complement(5882..6358)
                     /gene="ybeY"
                     /locus_tag="ACHAUN_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8460667.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6387..8579)
                     /locus_tag="ACHAUN_001983"
     CDS             complement(6387..8579)
                     /locus_tag="ACHAUN_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8460668.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8603..9574)
                     /locus_tag="ACHAUN_001984"
     CDS             complement(8603..9574)
                     /locus_tag="ACHAUN_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8460669.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10279..11658
                     /gene="brnQ"
                     /locus_tag="ACHAUN_001985"
     CDS             10279..11658
                     /gene="brnQ"
                     /locus_tag="ACHAUN_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8460670.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11940..13280
                     /locus_tag="ACHAUN_001986"
     CDS             11940..13280
                     /locus_tag="ACHAUN_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8460671.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         3707 a         2850 c         2451 g         4355 t
ORIGIN      
        1 tataatcata ccacatacag tttttttgac tagggaaatt cctaatttaa aagtaaataa
       61 aacagtaaca agattataat taatcgtttt catttttatt tcctcacttt tcatgagcgt
      121 tttgaaaatt cgaacaaaga ataaaataac aaaaattatt aaaatagaat taataacagc
      181 tatttataac aaaaaaaaca gagacacagc acgtttcgtg catgctctct gtttttcaaa
      241 caaaagacta ttttgtgatg atttgatcca agcttgctaa tgacaaagcc atggcagcta
      301 tttgacttag ttgtgttagt cggttcttac gtactgctgg ttcatcagcc atgaccatcg
      361 tttgatcaaa ataattttcg atcagtggac gaagagcgac aagctggtcg tagttttcgt
      421 ggatcgtttg gtcagcaaaa cttgctttca ttgcttctac tgctttgtgt aattctttct
      481 ctgcgtcgtt ttcgaacagc tcagggtcta cttctgattg tttttcaagc tgcgcttttt
      541 tcgctaagtt cacgacacgt gtcaaagctt ccattgaagg tttgaagtct tcatctgcaa
      601 gatgttgttt gaacaattgt gctgctgcaa atacttttgt cagatctttt tgttccgcat
      661 tcaaaacagc atcgatgaca tcatgacgaa tattcttcgt tgtcaataat tggcgcagac
      721 ggcctttgat aaagtcgatc acttgttgtt gcccttctga taaaccaatg ccgaaacgat
      781 ccgtatcttt actgataaca tcgctaatcg tttcttgcag aacagacaaa ggaaaggccc
      841 attttttcga ttcgatgatc cgaacgacac cgtatgcttg gcgacgtaag gcataaggat
      901 cgtttgaccc gcttggaatc atcccaactg caaagaaaga aaagatgcta tctaatttat
      961 ctgcaatcgc taaaacagaa cctactgtag actgaggtag ctctccttca ctggatatag
     1021 gcatgtaatg ctcacgaatc gctgtcgcta cagcaggttt ctcgcctttc agaagagcat
     1081 atttttcgcc catgatccct tgtagttcag gaaattcccc taccatgttg gttactagat
     1141 caaatttata gatttcagca gctcgttgca aatccatcaa ttcttcctca gatagtccta
     1201 cttgttcgcc aatgatacgg gcaatgactt gtacccgttg cattttctca tagatactac
     1261 cgattttttc atggaaagta acagttttta gtttttcaac gtattggtcg attgaagctt
     1321 tcttgtcttc attataaaag aattccgcat cttctaaacg agccgtcaat actttttgat
     1381 tgcctcgaac cacatcttcc aattttaccc gatcaccatt acgagcagaa ataaaatgag
     1441 gaagcagcat accttgatca ttccgtacct cgaaataacg ttgatgttct ttcatagaag
     1501 tgactagtac ttcttcagga acagaaagat atttttcttc gaagtcgcca gaaaagacag
     1561 ttggatattc gactaaattg ttgacttctt ctagtaaatc ttcgtctaga tcaataaccc
     1621 agtgattttt ttctgctaat tcgtttgctt gtgtaacgat catttcttgt cttttttgcg
     1681 gagacgcaat gacgaattgt tcattcaatt tttcttcata ttcttttgca tgcgcaaatg
     1741 tcacatcttc gcctaagaaa cgatgacctc gagaggctct gcctgttttg acatccaaga
     1801 tttcgaatgg gattacttcg tcatctaaca atgccacgat ccagtgaata gggcggatat
     1861 attcaaaatc gtattctgcc caatgcatcg ttacagggaa agtcaaactt ttgatcaccc
     1921 ctgctaagcc tgtcaatact tcttttgctt ccttaccatg agtgtatttc gttacataga
     1981 catattccac accttttaat tctctaaaag tgatagcgtc tgtcgtcaga ccttgtccgc
     2041 ggacaaatcc ttccgctgct ttcgtccagt ttccatctgc gtccaaagca atttttttgg
     2101 ctggtccttt gacttcttct tcactgtccg cttgtcgttc tggaatattt gttactttga
     2161 ttgctaaacg gcgcggtgtg gaaaatgttt cgatcgtatc ataagtcaat tggttttcat
     2221 ctaaaaattt tgctgttttt tcttctaatt gtttcatgct tggtgtgaca acatgggcag
     2281 gcatttcttc cagcccgatt tcaagtaata agtcttttgc catcttattc agcctccttc
     2341 acggtttctg gatttgctgt gtttttattc aacaaaggga atcctaattt ttcgcgttct
     2401 gccacaaaga ttttggctac agcacgagcc atgttgcgga tgcgagaaag ataaccggca
     2461 cgctctgtta cggatactgc accgcgggcg tctaataagt tgaatgtatg gctacatttc
     2521 aagatataat catacgccgg atgaacaaga ttttcatcga tacagcgttt agcttctttt
     2581 tcaaaacgat caaagttgct tagtagcaag tcttggtcgc ttacttcaaa agaatatttg
     2641 gaatgttcat attctggttg tttgaagatc tcgccatatt tcacgccatc agcccattct
     2701 aaatcataga cactttcaac ttcttggata taagaagcaa gacgttctaa tccataagtg
     2761 atctcagaag ttactggttt acaaggcagt ccaccaactt gctggaagta cgtgaattgt
     2821 gtgatttcca ttccgtcaag ccatacttcc caacccacac cggcacagcc catggatggg
     2881 ttctcccagt tgtcttcaac aaaacggatg tcgtgttcta atggatcgat tcctaaaagg
     2941 cgcaagcttt ctaaataaag ctcttgaata ttctcaggtg aaggtttcat caccacttgg
     3001 aattgatgat gctgatacaa acggtttggg ttttcaccat aacgtccatc agctggtcgg
     3061 cgagatggct ctacgtaagc agcattccat ggttctggtc cgattgcccg taaaaaggta
     3121 tatgggctca tcgtacctgc tcctttttct gtgtcataag cttgcatcag catgcagccg
     3181 tttgatgacc aaaatttttg taatgttaaa atcatatctt ggacggtcaa ttttttactc
     3241 atctgttttg cccctttcaa ttttaaagcg ttgctgtcaa ataagaatac aggaaagaaa
     3301 acgacaaaaa atccctatgt ctgattacag acatagggac gaataattcg cggttccacc
     3361 ctacttccga ataaagattc ggcagcttca tttatcgtac tcgcaagtgc ctcttcaaaa
     3421 atcgtttggg tccggctccc actctcccga actcgctaaa ccacgtgttt tttactgatc
     3481 ttggtcaatg tacgtattca ttttgcagtt ttcttgtttc tataatagat cctatcactg
     3541 aacgatctat ttaatctatg gttaataata gacggaactc cctgtgtttg tcaaatggtt
     3601 ttgatttttt gttgtatgtt ttatttacta ttcttctttt tcaggtgtct ctttttcttt
     3661 ttcagacatg tctttctctg acgtctttaa tacgtgtgcc caagacttca tttgattgat
     3721 gaactttttg cttttcaaat ggataccgac atattcatca tacagctggt cgatgagttc
     3781 acggattgct tgtttggttt cttctttcag atggattcca ctaattttat cataagaaat
     3841 agctgaaaac atccgaacga aataaacagc tcgaggatct gcatgataac ggtgatcgtc
     3901 catcgaccaa tgtctttcgc atatcacacc accatatttg gaagaatagt caaattttcc
     3961 ttttgtttcc ccgcatacga cgcagtgtgt ccagattggt gcaattccga agcgttgcag
     4021 tatttggact tcaaaaatat tcgtgatgat ttctgcatcg tctccttggt ccatccgttg
     4081 cagtgcttgt tgcgtaaact ggaacaaatg cgggtcgtac tgatgatctt cgattgccgc
     4141 atctactaga ttcaataaat atgtgccata gccgctcaaa aagatgtctt cttggatgtg
     4201 tcgaaacgca tggacatctt tgacactgct taaaaatgac aaaccttcac ttcgaaaatt
     4261 cccgatataa actgcttcag taaaagggag caaagccgga gctaagggct gattttgccg
     4321 atggatctct ttgatataaa acatcatttt gccagaagat tctgtaaaga ttttgactaa
     4381 tttatctttt tctttgtaat tacggctaga taaaatcaag ccttttgatt ctgcaagctg
     4441 gctcatattc tccctcctaa aaaataaatc tggaaaagca gattctgccg atccagatca
     4501 tttatgttgc gatcttccgc agcttttctc aacacgcgga tttttattca gtttatttct
     4561 atacctaatt tacaagaagc tcagaatatc agtattcgtc ttttcggtag ccgaagtctt
     4621 gcagatagac ttttttgtct cgccagtctt tttgaacttt gacccaaagt tctagaaaga
     4681 ctttgtctcc taaaagattt tcgatatctt ttcttgcttt cgttcctact tgcttcagca
     4741 tttttccgcc tttgccgata atgatccctt tttggctatc tctttcgaca ataatcgtag
     4801 cctggatatg gattttatca ttctcgtttc gcttcatcga atcaacaacg actgcgacag
     4861 aatgaggcac ttcgtctcgt gttaaaaaca agactttctc acggatcaat tcagaaacga
     4921 tgaagtattc aggatgatca gtcacttgat catctggaaa atattgtggg ccctctggca
     4981 tttcactgac taatacatcc atcaaacgtt cgacgttgtt tccttctgta gcagagattg
     5041 gaatcacttg tgcaaaaggc atctgcgttg aataatcttc gatgattccc aacaactcgt
     5101 ctggatggat tttgtcgatt ttattgataa tcagataaac aggagaatgc acgtttttta
     5161 gacgttcaat aataaaatcg tctccccgac cgcgtttttg atcagcacta atcataaata
     5221 agaccgcgtc cacttctttc aacgcactgt aagctgcttc taccataaaa tctcctaaac
     5281 gatgctttgg tttatggatt cctggtgtat caataaaaat caactgagct tctggtgtag
     5341 tgtatactcc ttggatttta tttctagttg tctgtgcctt gtcactcatg atggcaattt
     5401 tttgacccac gattcgattc aataatgtgg atttccctac atttgggcgg ccgacgattg
     5461 cgacaaaccc tgatttatgt tctggcatat atgttctcct ttttaattaa acaacaaatg
     5521 atatatttta gggacaaata aaatcaaccc aacgatgacg gtaaaacaag cggtcactaa
     5581 aactgctcca gctgccatat ctttgatttt tttcccaatc ggatgaaaat gaaaatccgt
     5641 aaacatgtcc acgacatttt caaaaacagt attgatgatc tctactatcc atacgagaaa
     5701 aacagctaaa agcagccata accattctat ccgatcaagt tggaaaatca cgcccataaa
     5761 caaagcgagg attccgaacg caacatgttt cctcatgttg cgttcttcct caaaaacagt
     5821 tttgactcct tggacggcaa attccacgga catcaggaaa tgtttgtttt tctctacgtt
     5881 tttatctttt aagtccatag gcatctaaga tctctttctg taaaccaaac atttctttct
     5941 catcctctgg tgtcatatgg tcatacccgt tgatatgcaa gaatccatga accgctaaaa
     6001 agccaagttc ccgatcatac gaatggccgt aagcttctgc ttgctctgca gcacgttcag
     6061 tagagatcat gatgtctccc agtacacgag gcaatggatt ctctccatct tcaaaaatca
     6121 ctgggatttc gtcttcgccc tcttcttcca aggcaaaact gatgacgtct gtcggtgcat
     6181 cttttccacg gtaatcacga ttgattactt ggattgctgc attgtccatg aaagtaacag
     6241 acatttctgt ttcttctggc aactctaaat atgatgctgc aaattgcagc aagtcgtcga
     6301 tttctttcat tttctcttct gagacttggt ttgtttcatc catgaaggta atatccatta
     6361 tacatcgctt ctttctattc ttcttgctat tcttcttgtt cttcttttat cttctcaact
     6421 tccgatttca ttttaggata tttgatccga gaatggaagg tactgcttaa accgctgatc
     6481 aatgattttt ctactttacg gatttctttt aacgtcaatc cactgtcgtc caattgtcca
     6541 tcggaaatac gctcttcaat aagattatgg acaaaacttt cgattttttc gctcgttgga
     6601 tggtccattg cccgtactgc cgcttcacaa ctgtcagcta tattgacgat tcctgcttca
     6661 cggctttgag gttttggtcc aggataacgg aattgctctt ccgtaacttc cgggttgcgt
     6721 tcctttgcct taacgtagaa ataacgcatc agcgtggttc catgatgctg tctacaaata
     6781 tcgatcacca tttgcggcat attataatct tccaatattt tggcaccatc tgtcacatgt
     6841 ccaaagataa tatgtttact gtcttctggc aataagaaat tgtgcggatt ttccgctcct
     6901 gaaggcaagt tctcaacgaa aaagctggcg tgtttgatct tgccgatatc atgataatag
     6961 caagctactc gagttagtaa agaacgtcca ccgatttctg ctacagcatt agcacttaga
     7021 ttggctacca tcatgctatg gtgatacgta cctggtgctt cttccagcaa ctgtttcaat
     7081 aaaggatgat tgggattgct gagttcattt aatacgatca cactatcatc attgaccatc
     7141 aattcaatat aggggtgcag acctatcgtc agcaagaatg aaaacagtgc acctgtgaat
     7201 ccgcagacta gcgttaacca agtcgttgca tccccaaagc tcattccttg gtagataatc
     7261 aaaatgacat ccatcatcac aggaaaaata atgacccaca tagcagcgga taacccttgt
     7321 tctgagatcc gtttgcgtct gacgatcgtt gccatgagac cagaaaatac ataggacatc
     7381 aaaataactg tcagaaagtt cgtgccaatc gaatcataaa aaatgaaaag tgcggatact
     7441 gcttgaaaaa taccagccat gatccctgct cttcgattca agaaaaagct taaaacaagt
     7501 ggagcaaaag ccgcggggta aaacagcgga atataagcag cttgttctgt ttggaagagt
     7561 tggaaaaatt tcattagtac gacgcttact gacattgccg tcacgtagaa aagtacgtat
     7621 tttgttcttt ctcctgcatt ttggaattga agtgaattat aaatcagtac accgatttga
     7681 agcaaaacag ctaaaaccat ggcaatgagt gggaaaatag aagtcgtttg attcgtcaat
     7741 cctagtaatt ccaatttttt catcgctgaa gcatcaattt gatttccttc gcgaacaata
     7801 atttctcctt ggaagatcat cactggttgt acagcttctc gtgcagattg ttttaattct
     7861 tctgtctttt tctcattcaa tacatcatta actacgatgc cttgatccac taagtagcga
     7921 atcatttgtt gctgctctgt tgtgacattc aaatattgaa tctgatcttc tgcttcttgt
     7981 ttaaaagctt ccaattcgct ttcacgaact tgtttactca tctgttcgtc aatgagcttc
     8041 aaactttcat ccctaactgt gtgaatttcc gtttcagtca tttcaaatat cgtcgaataa
     8101 aaattgttcg gcagtttttg gtaaaacgca acgttttctg cattcacgtt ttcaaaattt
     8161 ttcttcagtg tagcaatccg ctcttctgta gtaggtttcg ggatattttc atcttccttc
     8221 gccttatcta ccttgccatt ataagatttg ttgatggtat cattcgtctt atcgatcaat
     8281 tcgaataatt gcttgatccg attgttctgg tcatctgcca gatctttttg ataagtatat
     8341 tctggagtca ctgcttctgc tgccagtttt cttctttgtt cggtttcttc cgtgttctca
     8401 atcgttttat tcgcacggat actctcttct gctacttggc cttctctgaa atcaatggcc
     8461 ttttgtttga cactactaaa tgtaacgccg cagacaatga taaaaaacaa gaacagaagg
     8521 aaaggcaatg ctgcctttcc gagtttttct tgtagtttct gcagtgtttt attcaacatg
     8581 tgcttatcct cctaagcaaa gcttattctt gatgaagatc cgccttttca taagctttga
     8641 tgatttcggc aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga
     8701 tttttttgat ttgctttaat gttcgttctg catgaacaag accacttgtc gtcccacgag
     8761 gcaagtctat ctgacttgta tccccattga caatcatttt tgattgataa ccgagacggg
     8821 tcaagaacat tttcatctga gcgactgtcg tgttttgtgc ttcgtccaag ataacgaatg
     8881 catcatccaa tgtccgcccc cgcatgtaag ctaaaggtgc gatttcgatc acgcctcgtt
     8941 ccatcaatcg gttcgtatga tccaatccaa aaatttggta taaagcatca taaacaggcc
     9001 gcagataagg atccactttt tctttcagat ccccgggtag aaagcctagg ttttcccctg
     9061 cctccacagc gggccgcgtc aaaatgattt tttgcacttc gccttttttt agggcagcaa
     9121 ttgctaatac tacggcaaga aacgttttcc ctgttccggc tggccctaca ccaaatacga
     9181 tgtcatgttt ggctaccgat tcaacgtatt tcttttgacc gctgtttttt acccgtatag
     9241 gttttccatt acggtctttg acgatctctt cctcatacat ctcaacaaaa taatctaatg
     9301 tacctttttg ccccattttc aatgccgtca caacatctgg cgtacttacg tggattcctc
     9361 gtttgatcaa ttcctgtaat gtccgaatga ctgaggcagc caaggaaatc tgggattgct
     9421 caccaatgat ctgaatgatt tctccacgtg tatggatggt tgtttccgtt gtttcctcga
     9481 tcagttttat atgcttgtcg tgtgcaccta aaagcatgct gacatcatca gcagctgtca
     9541 atctaatctc taaagaacta gactcttccg tcaaaaatag acatcccctt tttttcgatt
     9601 cattctttcc tattttacca tactcagaag aaactatata gcaaataaga aaaggttgta
     9661 aaagagtcgc ttaaccatac gtaccaagaa acgttaaaaa atagccgttt ccattcgcta
     9721 aaggtctcgt tttgataaaa agaagctgag aatgtaagtt atatgaaagt ggagaacaaa
     9781 atatttcgtt tcattcaaca agtattctct aggtattttt cctgtttcaa aaaaagaaaa
     9841 caaaatagtt aaaatattct tgcatttcaa aaaaaacagg tgtattatta cgtcaacaaa
     9901 taaacataca gcaaagcgtc gaggaaaaca gtaggctatt caacgattta ttcccagtga
     9961 gtcatgtaag tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt
    10021 cgaaaacgta gatcattgtg gctgcctcca ttatcaggct agcggataac tttatcaaaa
    10081 agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta
    10141 gagccctttt cccaagtgga aaagggcttt tttttatttt aaggtggtgg ttttcatgga
    10201 atctgatatt agtggcatta ctaattgctg caacgtttat atcgtcaatc aaaatgataa
    10261 ttattggagg aaaaatttat ggaagaaaca aaaacttatt catggaaaca actattgatg
    10321 gtcagttcac ttatcttcgg gatgttcttc gggtcaggta atctaatctt tcctgtccac
    10381 ttaggtcaaa tggctggcgc aaactgggtc agtgctggcg taggatttgc tatctcaggt
    10441 gctcttttcc cattactagc tatcttagct gtagtagtga caaaaagtga tggtctgtat
    10501 gacttagcta aacctgtcgg aaaatggtat gctgccttgt tcttaatcct tgttcatttg
    10561 acgatcggac catttttcgg tacaccacgg acagctgcta ctgcttttga aatggctgct
    10621 aaaccatttt tgcctgaaaa atataattca ttaggtatgt ttttattctc tgctcttttc
    10681 tttttacttg cttatttcct tactgtccat ccaacacggt tggtcaaata cgtcggaaag
    10741 tatctaaact ccttgtttct tgcattatta gcaatcattt ttgtcgtttc tttattccat
    10801 ccaatgggaa atctaaatca atctgctgca gatacttatc aaacaaacgc tttaacaaat
    10861 ggtgtcctag aaggttataa cacggttgat gcagtagctt tgcttgcctt aagtgtcact
    10921 tttgtccatg cagtccgtga ccttggtttc aaagataaaa aagtcacaga attaacggca
    10981 aaagctggta gcctgagcgt tgcgctagaa gtcatgatct attttggact tgttcttctc
    11041 ggtgcgatga gtttgaataa gatgtcttta tcagaaaatg gcggaactgc actttctcag
    11101 atcactaatt tttatttagg taacttcggc gctagcttct taggagtgat ggtcacatta
    11161 ggtgtcttca ctaccgctat gggactcgtt gtttcttttg cgcaagattt ccataaactg
    11221 ttccctaaag tcagttatat gacatggctg cggttaacga ctttcgtctc ttttgtcgtt
    11281 gcaaatgctg gtttagacaa tatcatccaa tggtctttac cagtattgat gcttttgtat
    11341 ccattgtcgc ttgcgttgat cttgctttcc ttaacagcaa aatttttcca aaagacacca
    11401 tttgtctatc aagtaacgat gttgtttgct gctgttcctg ccgtattgga tatgctggca
    11461 aattcgcctg ctttagtgag tcaacaaaga gttgtagcaa gcatgctcga attttatcat
    11521 catcacgttc cttttgctgc attaggactt ggctggatgg tcccaacgtt acttggttac
    11581 gctggcagcc tactgtttta ttacgcctat cgcctatcag ggtataaaca agaagcaaat
    11641 gaactaccag aagaataacc aaatcaaaca caatttaaca taagaaatat taaacaggaa
    11701 aaaaccagct agatgaaaga atcctattct ttcgtctagc tattttttag ttctcttttt
    11761 tacgcgcaca aaaaaactgc ggacaatgat tgtccgcagt ttaaatgggc tctataatat
    11821 ctagtgttgg tgacgccttc tgttgccaat actttttctt ttttctaata aacgcaaaca
    11881 gacaccttga aatcctttag aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga
    11941 tgtcctattc agagtttacc aaagagttac tagatattct agacctaaat cttatctttc
    12001 atgaagatgc ctttcgcaaa gagcgaatca atgacgaaac atgttttgtt tttgatggca
    12061 cattgaccta tcaaccagag gaatgcttcc attgtcacta tcaaaataag caaacgatca
    12121 ttaaatgggg ctggaaaaaa gtatccatct tattgaatga tgtcagtaac tacaagacca
    12181 ttctgcggat taacaaacag cgcttcaaat gtaaacactg tggaaagact tttttagcag
    12241 aagattcagt tagtgatcgt cgctgctcca ttgctcgcag agtcaaacaa gccattcttg
    12301 agctattaag cgaacctctt tctatgtctt tgatcgcacg tatgaagcac atttctccga
    12361 ccactgtcat tcgaatactt cgcagccttc ggcctaaaac cgtttctttg aaccagccgt
    12421 taccagaagt ggtgtgtttc gatgaattca aatcggtgaa aaacgtctcg ggagccatga
    12481 gctttgtcat gatggacggc aagacccatc aactgattga tattgtggaa aatcggcaac
    12541 taaatccttt acgcgactat ttcttgcgct accctagaaa agtccgtgaa caagtacgtc
    12601 tagtggtttc agatttctat acaccctatc gcacattggt gaaagagtgc tttccgaatg
    12661 cccgaatcgt cgccgatcgc tttcatatta gccaacacat cggtcgggcc tttaccaacc
    12721 accgaattca agtaatgaag acctttaaaa aaagggatcg tcggtcgaag cacttaaaga
    12781 aatactggag actacttcaa aagaatgcgt gggaattgaa aggtcagcat cgctactggc
    12841 gtccgtcatt tcgagatcat ctgacagaag cagagatagt cgatcggcta ttatcttatg
    12901 atgactcctt aaagcgaggc tacgaagtct accaggattt tctttccgct atcagaagac
    12961 aagatgttcc tgaattcgtc gcgctcctaa aagaagacta caaggagttg ccagagcact
    13021 accaaccagt gttcacgacc ttcaagaaat atcgaacaga aatcaaacga gcgttgcgag
    13081 ttccctattc caacggccct atagaatgtt tgaacaacca tatcaaagta ctaaaacgaa
    13141 ttgcctatgg ctttagaaac tttcaaaact accaagaacg aatcttttta tatcgaggaa
    13201 aatattttaa gaaaactaag aacactaccc aactgactaa agctagaaca acaacacggc
    13261 tagacaagat agctgtctag ccgtgtgagt atctcgatta ttctattgaa tttcggctct
    13321 atactttttg atgttggtga acttcaccaa catctttttt gta
//
LOCUS       DAYYBG010000068        13356 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000068 DAYYBG010000000
VERSION     DAYYBG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.069"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..359)
                     /locus_tag="ACHAUN_001987"
     CDS             complement(153..359)
                     /locus_tag="ACHAUN_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460672.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(375..989)
                     /locus_tag="ACHAUN_001988"
     CDS             complement(375..989)
                     /locus_tag="ACHAUN_001988"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8460673.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1136..1819)
                     /locus_tag="ACHAUN_001989"
     CDS             complement(1136..1819)
                     /locus_tag="ACHAUN_001989"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8460674.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1928..2386)
                     /locus_tag="ACHAUN_001990"
     CDS             complement(1928..2386)
                     /locus_tag="ACHAUN_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8460675.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2470..4233)
                     /locus_tag="ACHAUN_001991"
     CDS             complement(2470..4233)
                     /locus_tag="ACHAUN_001991"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8460676.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4454..5914)
                     /locus_tag="ACHAUN_001992"
     CDS             complement(4454..5914)
                     /locus_tag="ACHAUN_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460677.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6122..7048)
                     /gene="glsA"
                     /locus_tag="ACHAUN_001993"
     CDS             complement(6122..7048)
                     /gene="glsA"
                     /locus_tag="ACHAUN_001993"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8460678.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(7071..8498)
                     /locus_tag="ACHAUN_001994"
     CDS             complement(7071..8498)
                     /locus_tag="ACHAUN_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8460679.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8529..9743)
                     /locus_tag="ACHAUN_001995"
     CDS             complement(8529..9743)
                     /locus_tag="ACHAUN_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8460680.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            10093..11172
                     /locus_tag="ACHAUN_001996"
     CDS             10093..11172
                     /locus_tag="ACHAUN_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8460681.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(11473..11715)
                     /locus_tag="ACHAUN_001997"
     CDS             complement(11473..11715)
                     /locus_tag="ACHAUN_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8460682.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(11747..12304)
                     /locus_tag="ACHAUN_001998"
     CDS             complement(11747..12304)
                     /locus_tag="ACHAUN_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8460683.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(12317..12505)
                     /locus_tag="ACHAUN_001999"
     CDS             complement(12317..12505)
                     /locus_tag="ACHAUN_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8460684.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(12518..13084)
                     /gene="amaP"
                     /locus_tag="ACHAUN_002000"
     CDS             complement(12518..13084)
                     /gene="amaP"
                     /locus_tag="ACHAUN_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8460685.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3898 a         2825 c         2329 g         4304 t
ORIGIN      
        1 aaatttaaca atctttaata ttttttagaa aaaaataaca aataagttga aagaatcccg
       61 ttgcaaattc tttcagttgc accaggattc tttgataatt ttttagtttt atgtgttttt
      121 ttatatgttt tttcactttt cggaataaat atttatttgt taaagtaatt aaacaatccg
      181 ctaatcgttt gatctggcat ttctatttcc ttaccatcta aagtaacagt agtaacgaca
      241 aaatggtctt gcaatggcgg aacttctaag tactgccctt gcagaaaatc attcactgtt
      301 tcatattctt ttttcatttc ttttgaatct gtcgatggtt tatagcttat ttttaacata
      361 actagtgctc ctttttattc gggacgtgca atcgtgaaaa tttctcctat tgctgcataa
      421 tcatttcctg ccaaacggct tacagcattc aattttcttg gatcgatcct tcctgtctta
      481 tcatcataga ctgtttcatt taaatgatat ttctgtactt ctagcaaaaa taaatcagct
      541 gttactttat ttttattccg tatctcgaca tgttcatata aggatgtttc aaaacgaact
      601 tttgcttctg tcaatgctgg tactgaaatg attactgaat cagttaaagt caagtcagtc
      661 aggtacagtt cgctttcctc attaggcaga ttggctgctg ttttatttac ttcttctacg
      721 ttgtcctcat caaggatatg gataacagct tctcttgtct caagaatatt ctttgctgtg
      781 tcttttactg catcttgttt tctctggatc gccaaggata agatgggtcg attcgaactg
      841 acgatattga agtaactgaa gggtgcgata ttcactatat cttttgctga tttagtcgtt
      901 actactgcta ctggtctagg gatgactgaa ccaatcagca acttgtaatt ttcacgttct
      961 gtcaattctg aaggactccg ttcaaacatg gtttcgcctt ctttctataa aattcgttaa
     1021 aaagcagatc ttgaagacaa aatgaaaagt aatgcttttt tcatcttctt agaatctgct
     1081 ttaaactatc tatcaatttt tcttttttgt caagaatctg tgccgtgcgt gttttttagt
     1141 tatttgcttt acttgcggct tcgacatatc ttttagtgat caattgcgga cgggtgataa
     1201 tagatccaac tacgactgaa aaagcaccta attcatatgc tttctctact tgttcaggag
     1261 agtcagtatg tccttccata attactggga tatcgacttg ttctatgatt tgattcaata
     1321 aatcaaaatc aggtgtctgc gtctttcgtg aatattctgt atagcctgct aaagtcgtag
     1381 acaaaagatc aaaaccatct tcttgcgctt gaagcgcttc ctcaaaagtc gaaatatctg
     1441 ccatagctaa ccgaccgtgt tcgtgaatca tttggacaag ttcagcagtt gttttttctg
     1501 tcggacgttt acgagaagtt gcatccagtg caatgatttc tgcttgcgtc tccaaaagtt
     1561 cttccacttc tttttctgtc gcagtaataa acacttctga cccatcgtaa tctcttttga
     1621 taatccctat taccggtaaa tcaacgattc gcttgatttc attgatatct tctgtcgtat
     1681 ttgctcgaat accaactgct ccgccttctt ttgcagccaa agccattctc cccataataa
     1741 aagaactgtg tagcggctcg ttttccaaag cttgacaaga aacgacgact ccgcctttga
     1801 tttgttccag tgtatccaca atatctcctc cagtaatgtt atataaaaat acagcttcat
     1861 ataaaaatgt aacccttttc tattcaggag acaataatga gctatttctt tcagcagacc
     1921 tttcttacta aaagaaaatt acttcaaatt cttcgtatac gaccagcttc tcttctgtaa
     1981 attctatgtt caacgcttct ttataagcat gtttaaaaaa ctcagcatgg actttccgcc
     2041 aataagctaa tgtccgatcc ccttctcctt ctttccaagc atgctctttc gtgacttcac
     2101 taaaaggcaa gatggtcaca tttatatttt tgatgataca aactggttca ttttttccat
     2161 tcaggaccac gtctaattga ccaacttttg gtaaaggctc attctcttgt ttatacagat
     2221 cgtaaccact tgatgtacca gtctttatct tttttacaac tagttcgctc aactcgtctg
     2281 ccattttttc tgtatcccca aatgaaaaga aacctaattt ttctgattga taactaggat
     2341 atttttcttg gaactttttg acatattctt ctacttgctg atacataaca tccacccctt
     2401 tgctttttat aatgaaaaaa ccatccagat actagtataa acaattatct ggatggtgag
     2461 cataatgaat tattgaatat tcaagacacg cagactatct tcaacgattt gttttggtgt
     2521 taaatgataa cgttcataaa gctgccctac aggaacgcta tccgtgaatt ctttctctgc
     2581 accaaaattt aatacgttca ttgaagaatc accataataa cgactgatat tttcgccaaa
     2641 tcctcctgac aaactaccat cttctaatgt gatgactaat tcgtgtgttt cttttaattt
     2701 atctaatgtt tctacgtcat tttctgtgat cgttcctgga ttgatcaacg ttgcttcgat
     2761 tccttttttt ctaaaagctt ttactgtttc ttttcctaat tcaagaaaac cgcctaaagc
     2821 taaaattgct acttttcctc cattatttac tgtttgatag gctggaatac tataatcatt
     2881 tgcgatagct gtaccatgtt ctacaccatg tgtaggaatt cggattgcta caggctgcgc
     2941 tgtttgttcg aacgcccaat ctagcataga gatcatatcc tcttttgttg ctggagcaag
     3001 atatttgatg ttcgggatac ttgataagta aggaatgtca aatgttcctt ggtgtgttcg
     3061 gtcactagct gagatcgttc cgccaccgac taaaataatc actggattct cgttgatcgc
     3121 taaatcatgt gataattgat catatgcccg ttgcaagaaa gtacttgatt ggaagacgac
     3181 tggacgtact ccctgagaag ccaaagcaga agcgaaagtg atagagtgct gttcagcgat
     3241 ccccacgtca aaataatgat ctggatgttt ttcttggaat gttttcagtc caaaggcgcc
     3301 tggtatcgct gcattgactg cggcaatagg agctgtactt gtttgcattt tcttatccaa
     3361 ataatccatg atgatcatat taaatgtttc tccagaagtt acttgctttg cctccccggt
     3421 ggacagatca aatggtacat gccaatgata tttcatttta ttatcgattg ccggctgata
     3481 accgcgacct ttttccgtat ggacgtgaag tacaatcgga tgatcaatat cttttacttc
     3541 ttcaaataaa cggatcaccg tttccagatc gtttccatta tccacgtaac gataatcaaa
     3601 tcccattgct ttaaataggt tattgctgct tttaccgttg gtttgacgaa gctcagctaa
     3661 gtttttatat aaactgccat gattctcatc aatagacatc tcattatcgt ttactaatac
     3721 gattagattg gattgtaaaa caccaccatt gttcaatcct tcaaaagcta agccaccact
     3781 tagggagccg tcaccgataa atgctaccac atttcctgat tctttcttca gatctctcgc
     3841 tttagctaaa cctgttgcta agctgataga tgtagatgtg tgccccactg taaaatagtc
     3901 atgtttactt tcatgttggt tggaatatcc gctcacttca cggtaatgtc ctttttcaaa
     3961 accatttttt cgaccagtta aaagcttatg accataagac tgatgggaaa catcccaaac
     4021 gattttatcc tttggtgaat caaatacata atgaaacgca atcgttaatt ctactacacc
     4081 tagattcgga ccaacgtgcc cacctacatc gctgtccttt tgcaagattt tttggcgcat
     4141 ctcttctgcc aactgtttca tttcctcaac agataatttt ttgacatctg cggggctatt
     4201 gattgcatcc aatacatgta atactgtact cataatacat tctctcctcc gtactatctt
     4261 cttttaatga aaatagattt gttacacttt atctattcta ctacttagag ttgactctaa
     4321 gtcaatagca ttttaaaaaa atttcagaga aagcttgtta tataagtaga tcttcgcacg
     4381 aatcaggact caaataaaaa ccttctacag acaattaaaa agctttgtag aaggtctcct
     4441 aattaaggtc tcatcaagaa atatgttttt ttaataggtg aagttcctga gcaatatgat
     4501 acaaatggat gtttgcacct tttaatgaga ttggatagat ctttgtcttt tcagtttcta
     4561 atccattttg atagctagca gcatcgcaca aaaccactct ttttttcgca catagacatt
     4621 catcaggcaa ttcttctaat gaacgaaata aaataggatt tatttcagcg atgccttctt
     4681 ccgcatacaa atgaaattgg gttcgataaa aaaattgttt gactgaactt tctgaaacaa
     4741 caaaaatttc tatttcctgt ctccccgctt gttcactgtt gatcaaagaa agacttttga
     4801 cgaattttcg aatgaaattt tcattccatc ttagttggat ttgatttttc tttttccact
     4861 cttccaatat ttctacaatt tttccgtaca gaagttgttc tttttctgat agaagataga
     4921 tcctttccgg taaaaatact tgtccatctg cccacaacgt acggacaaat tgtaaaaagg
     4981 attgcttgaa aataaaattt tctgcatcgt cgatttcagc gatactaata atctgtaaaa
     5041 tcaattcatt cagtgatagg tcattgagaa caaaattttt ataaacaact tcgaaatctt
     5101 ttcttaacag ctccatgttc tcattggtat aatttctact gttgaacaaa gaaaaaatat
     5161 aatagacatc ttcttcttgt aagtctagaa ttcctttttg ctcttccaat ccttgacaga
     5221 ctaattgaaa taacggcttc gtttctgcgc ttttcttatc ttcttctttc agtccaaggg
     5281 gaaatgcctt tcttccgatt gcgatctcta ttcctcgtat gataaaggac ttttcatctt
     5341 ccgaatatct tcttttaaaa aaatgagtct ctacataatc aatcaactcg ttacaagctt
     5401 cttctacata cggattttta ttttcatagc cttcccaatt gacataacgt actaaagcta
     5461 gcaagatgtt ccgcgagtca aattctggga taatgatttc tttatttttc aaataatcat
     5521 tttctttaaa aaaatccaat acgatttttt tgattgtata tactttgctc aaagaaataa
     5581 attcaagatc agccagttct gtactcgtga atataccatt aaaataataa cttaaaaact
     5641 ttaaaaaata agattgaccg tacagaaact gtgtgagctc agaaagagca ataccatgtt
     5701 tgaatacgag tttatactta tgcttctctc ttacttgttc ttttatacaa tattctagac
     5761 actcggctag actttctaga tcgtgaagga tcgttggtgt agtcaccctt agagaaatag
     5821 ctgtttgttc gatgtctatt tcttggaatt caaataattg ttcacacaga tacacttttc
     5881 taaatatatt tcgctcgatg taattttcca gcattcctga tacacgcctc cagtttttct
     5941 tatgatttta tttaatcata tctgtaaatc gtatttttgt ttccttttta tttagaaaag
     6001 ccctttcaaa aaccaataaa aaaagaaact gggacaaaag tcaaacaagc tcacaatttc
     6061 agcctcattg taggtagctt tgagccgttt ggacttttac cccagcctct ttcaagaatg
     6121 gttattcaaa gatgtcagcg tctagttctt tgactacatc tttcaacaat tggataccag
     6181 ctgcgctgtt accaaatgga tctagtgccg ggctgaagac acctatgccg taacgatttg
     6241 gcacagctgc catcaacccg ccgcctacac cgctttttgc aggtacacca acatggacgg
     6301 aaaactctcc tgattcatca tacaaaccag cagttgtcat aattgatttg acgattgtag
     6361 cactttcttc agtaatgatt tgtttgccat tccaaggagc aattccttta tttgccagca
     6421 cagcagctaa ttttgctatg cctgtagcgg tgacattgag ggagcattgt ctgaaataag
     6481 catcgagtat ctccggtacg tctccttcga tcatttgatt acctttcata tagtaagcaa
     6541 gagagcgatt gatatcacct gttcttgatt cagaatgata gatttcttca ttcaatgtga
     6601 tattaggatc atcacagatc tctttcataa atgacaagat tcgctcaaat ttttcatctg
     6661 catctttacc ggcaatcaac gaggttgttg caatcgctcc tgcattaaca aaaggattca
     6721 ttggatgttt gcgatgattg atttccatat tcatgataga attgaaggca aaaccggttg
     6781 gttccgtgtt gattgtttga aagacttttt ctattccgtt gtctttgatt gctaataaca
     6841 atacgggaac ttttgacatg ctttcgatgg caaagcgcac atcggcgtct cctccctcaa
     6901 tcagttgttt tgtatccaaa tcataaatcg ccatacctaa ttgtttggga tccacttcag
     6961 ctaatgctgg aatgtaatcc gccacttgtc ctgtttgata atagtggaaa ttttttccaa
     7021 tcaattgttc taattctttt acttccattt gaataaccag ctttcatttt ttatttttct
     7081 gcatgctgga atttatgacg gcatgcgtag atcgtaaatg gaataactaa gacgataaga
     7141 taagaaacaa tcagaatcgt ttcataaatc ccatgctgtc cagctgcgat actagcaggt
     7201 ggaacaaatg agatacaaaa agcaaaaagt gaagtcaaaa gacctaaaca agcaacgatt
     7261 acttttacaa cttttccgcc tggaacctga taggctcttt caagactgtc atctttaaaa
     7321 accagcttca aatagcccgc gaaaaacata aagtacaaca tcaggtagat tacaacagtc
     7381 aatgacatcg ctgcaagaaa tgaaagatta ttccccccgc cgcttaatgt aaagacggct
     7441 gcccagattg ttgcaacgat cccatttgcg atcatcaaat gagcaggaac accgtgtgtg
     7501 ttttcttttg caaaatattc aggaagtaat ccttcttctg ctgctacgtg aagtgctttt
     7561 gaaggagagg ttacccaaga agcaatttct ccgatgacac ctaaacaaac aagaatcgca
     7621 agaattttcg caatccatgc aagatttgga tcaatgtgtc ttaataggaa ttctaacgat
     7681 tgaatcaccc ctgtattaag agacaaacca tgttgaggta ctgtagtagc gacggtcaat
     7741 ccgccaaaag tatctaaaat gattgccaga ataaccaata caaacattgc taatggataa
     7801 tcctttttag ggttttccat ttcatttgca tgactggcag atgtttctac gcccatatat
     7861 gctaaaataa aggaaacaaa aacaaccagc gtaggcaatt tcgaaaaatc aggaatcaat
     7921 gttttcatag acagtatcgt ttgtagtgga gcgcctgaag cgacataatg gatccctaag
     7981 ccaaacaaga ctaatgctgg caacaagata ccagctacaa aaccgatccg agcaatcaat
     8041 gaagtgtatt tggttccttt taactgtgag agtaaaatag cccagaaaat aatcaatagg
     8101 attgccaatt tcagatattt attgttttgt atagcaccaa tacccgttgc ataggataat
     8161 gcaccagtta agaaatacag catggtcaag tagccaactg ttatctcaaa ccattggaaa
     8221 aagattgcgg caaaaccgaa tcgttttccc aatgtgcgac tgacccaagt atagacaccg
     8281 cctttttccc atcctttcac cgttgccatc tctgctgcgc acaatgccac agggataaac
     8341 catagcaaac cgcctaataa caagaaaaat actaagctga agcctgaggt cgcgaatgtg
     8401 ggatattcat ataatgaaac gaccattgag atggtcatag ccaaaaagcc aaagaacgtt
     8461 aatttttttg caggaatttt cgttttgctt tgttgcatga atcttcctct tttctatttg
     8521 aatttgtttc aattgagttt gttgccaaag aagaaaaagt ccaatccttc ttcttgttct
     8581 tcttttgtag tagtgatcgt tgtaaagtgc atcgccgatt ttaaaatcac tgttgtcagc
     8641 acataggcat acacggcagt aatgatgaca ccaatcactt gaatccctaa gaagtaccag
     8701 ctgcttggat tcgttagtaa atggctgtta gatgcaaaga tccctatcag aatcgtacct
     8761 gtgaatccgc ccatgccatg aacaccccac acatcaagtg catcatccca ttgtttcttt
     8821 ttacgaaaat caacagcagc atgacagacg atacccgcaa tgatcccaat cagcatggca
     8881 ctttttgctt ctacataacc ggcacaaggc gtaatggctg ccaatccagc aacggatcct
     8941 gttagtacgt cgacaaaatc aaagccattt ccttttactt ttgcaatgat cagccaagta
     9001 atcatcgcaa atgcaagagc gatgaatgtt gaaccaaatg cattgactgc ttgttcatct
     9061 gctcgaagtg caccaccgga attaaaaccg aaccagccaa accaaagcag tcctgtaccg
     9121 atagcagctg ccataagact gctatgctga cttctttctc ctaaaggaat ctttctttga
     9181 ccaagtgtga ggatacatgc taaagagccg aatccagctg tcgtgtggat gactgctcca
     9241 ccagcgaaat ctctaaatcc catctgatcc aaaaatccgc caccccatat ccagtggcaa
     9301 acagggaaat aaatcagcag attccaaaaa ataacaagta taatatagcc ttttaaattc
     9361 aatctccctg caaaagctcc tgtcatcaaa ggaacggtaa tcacgataaa catcagttgg
     9421 taaagaaaga acattaaaaa tggaatcgtt gcaccatgaa acatgttggg gaagcttgta
     9481 acatgtctca tcaaaaagaa atctgccggg tttccaataa caccgccgat atcccgtcca
     9541 aaggccaatc cgaatccacc aaatacccac ataatcgttg ttatcccaac ggagagaaaa
     9601 gattgagaca tgatcgttag tatattattc ttccgtgcta atccgccgta aaaaaaagct
     9661 agaccaggag tcatcaggca aaccattgct gtacataaaa tcatgaatgc tgtatctcct
     9721 gtattgatag gtaatgtatt catttttttc gcctcctttc tacgattacg agtataaaag
     9781 aaggtgtagt tgtttttttc ttctgttttt ttgaattaat acttgcattt ataaaagaca
     9841 gacaattaat aaaagcttga tgtgttgtgc tctttttcgt ttttcacaca aataaagaca
     9901 aaaaataaac ggtcttcttt ttatagaaaa ccgtttattt tcgtcttttt ttatttttaa
     9961 agatattttt tcataaggaa taaagaggcg gattttataa ttaacttagc catttggaaa
    10021 taaaaaaacg ccacgtgaat tccatgttat attgaagtta ccacaactct caatagacaa
    10081 ggatgaattc acatgacgca aaaccatgat aacacagttt cacgcaaggg aaaacaccta
    10141 acctactcag aacgttgcca aatcgccatc ctgaaaaagg aaaactattc caaccgtcag
    10201 gttgcggatg tcctgggctg cgtgccgcag accataaaca acgaaatcaa acgcgggacc
    10261 gttactcagc ttaaccgcca gaatcagaac gggaaggtct acgactatta tcatgacgtc
    10321 tacgacgccg atgccgggca ggcggcctac gagaagaacc gcttgaactg cggcagacgg
    10381 cccttatggg ccgacacgga cgccttcatt gaatgggcgg acgacatgat gctgttggaa
    10441 aaatggtccc cggacgtggt ggtgggcttt gccagaaaac acgaattgtt cgacccggcc
    10501 attatccctg ggaccaccac cctctatcag tggatcgacc gtggcatcat gaaaacgaag
    10561 aatatggacc tgctagaaaa actgtcccgc aaaccgaaat cgtcccgtca tggcaaccgc
    10621 ccgaataaac gggtgctagg gaaatccatt gaagagcgcc cggaggcggt tggtacccgg
    10681 aaaaccttcg gccattggga agtcgacacg gtcgtaggaa acaagaccaa gtcggatgcc
    10741 gtgctgctga cgttggtgga acggcagaca cgcttcgagg tcattctgaa gctggccggg
    10801 aaggatgccg aatcggtcaa ccaagcggtc cgacagctga gagaacgggc tggtgcgtac
    10861 ttcggccagc tgttcaagac ggttacctcg gacaacggct cggagttcgc gggactgcac
    10921 gatgccttgc aggatgtggc aaacatctac ttcagccacc cctatgcttc ctgggagcgc
    10981 gggacgagcg aaaaccaaca caagctgatt cgccgtttca tccccaaggg cagcccaatc
    11041 ggcaacgtga gcgaaaacca aatcttgcgg attcagcgtt ggatgaacga ttacccgcga
    11101 aagattttgg gctatgcaac gccgcatgac acgttcgtcc aggcatttag gcaggaacgg
    11161 ttgacggctt gacacgtggg cccttgacaa cgagcctccc tcggcatggc ttgtgtctgg
    11221 aaagctggcc ggggccagcc agtcgggaga ctaccatgcc tcagctcatt gtcaagggcc
    11281 cactcgatta gaaaaattag acggttggtg gtaacgagca accgggtcac catgggtggc
    11341 taacttaaac ttgaaattta cgtaaggaat aaagaatact tcatataaaa tatataaaaa
    11401 agaaccgcag cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa cataagtttt
    11461 tcttcaattt gcttataatt tacgtgcaaa gaatgaaacg actaatacta aaatgattgc
    11521 accgatcaat gatggaatga tagccattcc tgcaattgtt ggtccccaag ttcctagtaa
    11581 agcttgtcca attgctgaac ctactaaacc agcaatgatg tttgcgatga tccccattga
    11641 tgatccttta ttcgtgattg ctcctgcaat tgctccaatg ataccgccga cgattaatgc
    11701 ccaaataaat cccattttaa attcctccta actgacttct tacttcttat tgtacacgtg
    11761 gttcgttatt ttctttgact ttttgtgcac ctttgcctac agcagatttt gctttatctg
    11821 tttggcgtga agcaaattgt cctgtggatt ttgctgcttc tgttactttg tcttgtactg
    11881 tttcttgatc tttttggtat tcttcttgtg ttttgatgtc tgcaacattc acattcactt
    11941 cgataacatc taaatgtgtc atattgttta cttcagtact gatcaattct ttgatttggt
    12001 tgtaaatatc ttcgatgtct ttaccgtatt ctacgatcac atctagatcc acagctactt
    12061 gtttcttacc tacttctgta ttgatacctg ctgttacgtt atctgtgttg actaattttt
    12121 cagccatatt tgagaaaaat ccgccatcta atgtcaatag accatctaca tctttcaatg
    12181 cataaccgat gattttttgg atgactttgt cttcataagt caattcacca ttcatcatgc
    12241 ggttttctcc tttttcattt gccaaaactg atccatttgt tgtattcatg tttttgtttt
    12301 ccatctttgt ttcctcctat gatttaaaaa attgatcaac tattcctgac tgtttcaaat
    12361 aaaaaccgat tcctgcgcca attgctgtca tgattatgat caacaatgtc ttgaaaaatc
    12421 caatcgttaa ccataaaaca gctagcagta gtccaagacc cccaaagata atagggagct
    12481 tgtatgctga aaatagttct ttcattattt ggccctccta ttccactcga gagtgcttgt
    12541 agtctcttgt atcttcttgt tcttcataac cgctgtatgt gactgccact ttgaccgttt
    12601 cttttgttcc gagtacttta gttacttctt cttcgatatc ttgcatcaat acacctgttt
    12661 tccctactaa tgaagaagtt cgtttcaagt ctccgttgac atggatatgg attcgatttt
    12721 tcgttgaacg tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt aaatgggaac
    12781 gtaccaaccc ttcgatcgct tttttgtcga ttgttagttt gccatcttcc cgtttcataa
    12841 cgaaagttcc tcttgacttt ggatagaaaa gaacgactag caagatgata atagcaagaa
    12901 cggcaaatac tacagctgcc cagaaaatat attgttgcat gtagtaattc gttaaagtaa
    12961 agaagcgaaa tcgttccaat tgttcaggca taatatacgg cgcattgatc aacgcaatcg
    13021 ctagaaatac tgaaagtagc agtaaactca tgataacacc gattattttt aaccccttat
    13081 tcattttcca cctcctataa acttattttt accctgtaaa taactctacc gacaatcaca
    13141 gtatagatag cgaacagtta gcatagcatc agtaaataaa gcgctaccaa acattgctgg
    13201 atcataacgg cgttttattg ccagtctgat actgaaataa caattgctta cttattctct
    13261 atttactttt gattgctggc tatacttaaa ggatattatt caagattaaa aattgcaaag
    13321 attatgctca gttattatta caagattcta tttatt
//
LOCUS       DAYYBG010000069        13291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000069 DAYYBG010000000
VERSION     DAYYBG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.070"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..505)
                     /locus_tag="ACHAUN_002001"
     CDS             complement(<1..505)
                     /locus_tag="ACHAUN_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460686.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILIS"
     gene            471..2108
                     /locus_tag="ACHAUN_002002"
     CDS             471..2108
                     /locus_tag="ACHAUN_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460687.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="ACHAUN_002003"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="ACHAUN_002003"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8460688.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="ACHAUN_002004"
     CDS             complement(3184..3819)
                     /locus_tag="ACHAUN_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8460689.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5186)
                     /gene="sstT"
                     /locus_tag="ACHAUN_002005"
     CDS             complement(3966..5186)
                     /gene="sstT"
                     /locus_tag="ACHAUN_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8460690.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5405..6403
                     /locus_tag="ACHAUN_002006"
     CDS             5405..6403
                     /locus_tag="ACHAUN_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460691.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6599..8434
                     /locus_tag="ACHAUN_002007"
     CDS             6599..8434
                     /locus_tag="ACHAUN_002007"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8460692.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8494..9210)
                     /locus_tag="ACHAUN_002008"
     CDS             complement(8494..9210)
                     /locus_tag="ACHAUN_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8460693.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9437..9979)
                     /locus_tag="ACHAUN_002009"
     CDS             complement(9437..9979)
                     /locus_tag="ACHAUN_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8460694.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9984..10211)
                     /locus_tag="ACHAUN_002010"
     CDS             complement(9984..10211)
                     /locus_tag="ACHAUN_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8460695.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(10290..12170)
                     /locus_tag="ACHAUN_002011"
     CDS             complement(10290..12170)
                     /locus_tag="ACHAUN_002011"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8460696.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12359..12973)
                     /locus_tag="ACHAUN_002012"
     CDS             complement(12359..12973)
                     /locus_tag="ACHAUN_002012"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8460697.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            13115..>13291
                     /locus_tag="ACHAUN_002013"
     CDS             13115..>13291
                     /locus_tag="ACHAUN_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8460698.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         4176 a         2564 c         2093 g         4458 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctaaggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacatat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttg cttctctaag acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactaa caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataaacga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc gattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgatatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatgc tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacaggttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacggaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaataaaaga ctaacaaata aactgagcaa ggggttgtga
     8461 taaaagacat gtcacaaccc cttgtttatc tcattaattt gcttgtccat aaacgatttg
     8521 attaaagcca tttttcaatt cattattcgc tgtctcgttg aagtaaagat aattatcggg
     8581 attcccctga ttttgttgtg tcaccaatac ttttggctga ttttgctgaa acccgaatag
     8641 atagacgtgc tttttcagat atggttctgt ctctgcatcg gaatagacgg ctgtgatttg
     8701 atagtctgct tgacctgtcc cactttctga ccattctgca gaaacaggca ctccaccaat
     8761 agccatcttc cactccccgt tcaaaatagc ttgagggact ttaagaccat ataagtccac
     8821 actcatttga ttcgtataac ttttgtattg ttgtcccatt gtttggcccc atttggtcat
     8881 aaatgcctct aattgtgcgg atttgtctgc attccacagt gtttgttcaa cttgttcgct
     8941 gctttctgaa gaggaagatg aagcctctac agaagaactt gaagtagatg tttgagttgt
     9001 ttcagtcgtt ttggaagact ctgacgaagt agaggtatct gctgtttctg tacttgctgc
     9061 agttgacttg ctctctgtgc tagttgttgt agtagttgca actgttgctt tcgctgtttc
     9121 ttttgttgat tcttttttcg attcattccc gttggtacaa ccagctaaca aaaaagctag
     9181 accaattgtt actaaaatcc tttttttcaa ttaaaaaact cctttcgatt actcccactt
     9241 ctttattcta tcaaataagc aatgtgattt tattgatttt tcgtattttc tcatcaatct
     9301 ttcaaatctt ctaaaaactg agaagcaccc gctgatgttg tctgtaatac atcagcgggt
     9361 gcttctttta ttttaatctt cgtgattttc caatttacct cacgctttat atttgtattt
     9421 gaagaaacat agacttttag tcatcatcgt cttcctcatc caaatcttgt cggacagttt
     9481 ttccttggag tctttgtact tggtagatga tttctttatt gaaacttaaa agttcaacaa
     9541 gcaattgttg caagccatat ttttcttctt tttcagctaa tttgattgcc cgtgtgataa
     9601 agacattttg gaaatcaaaa tcttttgcaa agttttccaa cattgttgag gctggtaaat
     9661 atttgttttg accatgttcg taaatttttc cataacgcat gacttcttct aaagtagacg
     9721 gaacgatttc attttcaatc actaactcat gagagatttt attgaaccat ctcgcttctt
     9781 gatcagcata ttctgaaaaa agggtactta attcttgcga agatgtaccc aaaacataca
     9841 attgtgcttg gatcaatttc atttcttcta atttaaaatt tgctaataca tgccccacca
     9901 tggcgccagc agttggttta tgatggtcga tatctgcttg ttttgtttct tgaacaaatt
     9961 tttcttccac tatattcatt gatttacgcc tcttttactt ttgctgattg gacttcataa
    10021 cctaattttt ccacaactgc agctaattgt tcagggtgga cttcatctgg atcaaagttt
    10081 gctttgattt tgctcgcatt aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa
    10141 accgcatgtt cgattttttg catacagctt ggacaagcta gtgtatctaa ttgcataata
    10201 atttttttca tcgtagttct tccttccttt ttcttgatag tcttattcta gattacagct
    10261 tccataaaaa aattgattct tatcaagttt tacgattttt cttctttgct ttcgtgtcca
    10321 aaatacaata aacgcatcgc gttaaagata acgatcaaga tactagcttc gtgaacgaac
    10381 atcccactag ccatgtaaat aaacccggca aataagccgt tgagtaaaaa taagacgaca
    10441 ccaatggcaa tcactacatt ttcacgggta tttaaaacag ttttcttagc caagcggtag
    10501 gcatgaacca acgattcaaa attagattgc atcaagacaa cgtcactcgt ttcgatagca
    10561 acatccgttc ctgatcccat agcaatcccg atatcagccg cagccaaaga gggagaatca
    10621 ttgattccat ctccaataaa agcaacgcgc aatccttgtt cttggtattt tttcacaaat
    10681 tgaacctttt cgtctggtaa taattcagca tggacttcat caatgcctaa catatcagct
    10741 acataatcag cagtcatctg attatcacct gtcagcatca ctaactgttt tgctcctgct
    10801 tgtttcaact tagctaattg ttcagcgact tctggtcgaa tcacatctga cacacctatg
    10861 atttgagtga tttttttgtc gattgctaca ataatgaccg agctgcctgt ttgttgtaat
    10921 tgatgtaaat cttgtacttg ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg
    10981 gtgttgcccg cacaaactgt atgaccatca atctcagctg tgattccttg tcccttgatt
    11041 gtctgattat ctttgactgc taattgttga aaatcaatgt ctttctcctg agcataatgg
    11101 atcacggcgc gccccagagg atgatcggat aacgtttcta atcgagccac taaagctaag
    11161 ctgtcccctt catcctctgt atagttcttg atagtggata cagcagtagt tcctttggtc
    11221 aatgtaccag ttttatcaaa gacaaaagta tcgattttgg aaaaagtatc cattgcttca
    11281 ccgcctttga ctaagatgcc ccgtttcgct ccattaccga ttcctgctac attcgaaaca
    11341 ggcgcaccaa tgactaaagc gccaggacaa ccaagaacca aaacagtgat tgctaaacga
    11401 aaatcacgac taatcagacc aacaaccaac gcaataagta aaacggttgg agtataatac
    11461 tgagaaaatc gatcaatgaa tttttccgca tgggatttgg tatcttgtgc ttcttctact
    11521 aattcgataa ttttggcaaa ggtcgtatca tctccgacct tagttgcttc aatcgtcacg
    11581 tagccatctg ctaaaatcgt tccagagaag acttcatctc cactgatttt gacgacttgt
    11641 cgtgattcac cagtcactgc ggcttcatcc gcataccctt tcccgtcaat gactcgtccg
    11701 tccacaggaa tagctccacc agtttttact aagacgatat cgccttcttc cacgtcatcg
    11761 atttccactt cttcagtcgt tccatcttct tggacaacac tagcagtaga tggggccatc
    11821 tcggtcaaag ccttgattga ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta
    11881 ccaaacaaaa acaagaatgt aacgatagct gactccttgt attctccgat cataaaggca
    11941 ccaagcacag caatagtaac gagtaaatca attgagataa ctttgacttt cactgcctga
    12001 tacgcatgga tcataatagg tatgaccgca ataatagaag ctatggctag agcaatgtta
    12061 tagaacaaga caaaaccagt aaattttccg ataaaaccaa aaataatcaa cacagctgat
    12121 aaggcggtga tatgatttcg atgctcttgg atccactttt gtaatttcat ctttctccct
    12181 cttttccttt cattcttacg agactaagta tagatactta tccatcataa aaaattgacc
    12241 actatcaagt ttagtcaaaa aggcggtttt cttttggtag aatacactac aaaaaatagt
    12301 tagcaagaag actcactaaa agtgatcttg ccaactattg tcgtttctat cgaaattttc
    12361 attgaatgat tttcctgtac agcatacatc ggagatctat ttctttatct aagattttta
    12421 tcttgctaat tttttccttt agtctttcaa atccacgtcg ttcatagaat tgatacgtac
    12481 agccactatc tgtaaaaagg aatatttctt ttcctttctc tcttttttca aattctgtta
    12541 gcaacttggt accgatgcct tttgttttca aatcaggatt tgcagctaaa aaaatgatct
    12601 ctccatctgg cgaatgcttc tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt
    12661 aagctccttt actttcttta gcaaatatat tttgtaaaaa atcgaatatc ttaacatata
    12721 tttgctgtcc aaaagaacga tgtttctttt tttctccttt tatctccgcc agcaaaacac
    12781 ctgccaactc gtcctctgca tacaaagcaa gaatttgagt tgctcgattc aactccatat
    12841 acaaaaaata tcttccgtaa agattcagca gaagttcatt ttccatatat ctctcaaagt
    12901 tcattccttc aacagcaaaa tagatagctt ttttgtaatc tttcttttct aagggtttaa
    12961 aagttagttt catcatttcg cctcctcaac ttcaatgatt gtgttatact tacattataa
    13021 tagacacagt gtctactatc tataatcaat catctataag tttgtcgatt aagcgacagc
    13081 tttgctttga tatgtcggat aaggagatag gtaaatgaaa aagacagata gacgcgtcaa
    13141 aaaaacagaa aaagctttgg ctgaaacact atccacatta ttagtaaata aaaagattca
    13201 agcaatcact atacgcgaac taacagaaac ggcagatgta catcgttcga ctttttatac
    13261 acattataag gatatctatg atctatatga t
//
LOCUS       DAYYBG010000070        13156 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000070 DAYYBG010000000
VERSION     DAYYBG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.071"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1862
                     /gene="yfcC"
                     /locus_tag="ACHAUN_002014"
     CDS             123..1862
                     /gene="yfcC"
                     /locus_tag="ACHAUN_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8460699.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2019..3203
                     /gene="iadA"
                     /locus_tag="ACHAUN_002015"
     CDS             2019..3203
                     /gene="iadA"
                     /locus_tag="ACHAUN_002015"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8460700.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3314..3517
                     /locus_tag="ACHAUN_002016"
     CDS             3314..3517
                     /locus_tag="ACHAUN_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8460701.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3618..4280)
                     /locus_tag="ACHAUN_002017"
     CDS             complement(3618..4280)
                     /locus_tag="ACHAUN_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460702.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4293..5795)
                     /locus_tag="ACHAUN_002018"
     CDS             complement(4293..5795)
                     /locus_tag="ACHAUN_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8460703.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6343..8022
                     /locus_tag="ACHAUN_002019"
     CDS             6343..8022
                     /locus_tag="ACHAUN_002019"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8460704.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8149..8628
                     /locus_tag="ACHAUN_002020"
     CDS             8149..8628
                     /locus_tag="ACHAUN_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8460705.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8847..9527
                     /locus_tag="ACHAUN_002021"
     CDS             8847..9527
                     /locus_tag="ACHAUN_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8460706.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9535..10866
                     /locus_tag="ACHAUN_002022"
     CDS             9535..10866
                     /locus_tag="ACHAUN_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8460707.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10881..11843
                     /locus_tag="ACHAUN_002023"
     CDS             10881..11843
                     /locus_tag="ACHAUN_002023"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8460708.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11954..12292
                     /locus_tag="ACHAUN_002024"
     CDS             11954..12292
                     /locus_tag="ACHAUN_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8460709.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12371..13078
                     /locus_tag="ACHAUN_002025"
     CDS             12371..13078
                     /locus_tag="ACHAUN_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8460710.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4182 a         2321 c         2884 g         3769 t
ORIGIN      
        1 atagaaaaaa gtgattttct aataaattat tcatgtggaa atgtcatttt tactgtataa
       61 ttcatttgat tatccatttt ttattcatca aaattactga aaaaagaaag gaagaaataa
      121 atttgaagaa aacaaattct tcaggaaaac cgaaacgcga cttcactaaa ttatcgacac
      181 cccatacgta tgtcattatt tttggtgtag tcatctttgc atggatactg acttttgtcg
      241 ttccagcagg aaagttcagc acgcaagata ttgaatacaa agatgcaaat ggcgaaacaa
      301 gtactcgtac tgttttacgt caagattcct tccgttatgc ttacgagctg gataagtcgt
      361 atgtgtttga tcaattagaa gaactgcaag atcatccagc agaacgtgaa aaactggatg
      421 ttccggaaaa aggattggaa aaagtcattg ctgacggaga aaaaaatctt acgcaggaaa
      481 aactagatga aatctccttg acagatgatg tgctttacga tcagtacggc gaaaatatct
      541 atgatacttc taaaaaattg cataaaacag ccaaaatatg gggaacagat gattttggcg
      601 gctttggttt cttaaatttt gtttttgaag gactagtatc tggagataag tatggatctg
      661 ctgtagggat cgctgcattg atccttgttg ttggaggtgc ttttggtatt attatgcgta
      721 ccggagcgat cgacgcaggt atctatgcgt ttatcagtaa aactaaggga ctggagcgtt
      781 tagctttacc gctattattc tttgcttttt cattcggtgg cgctacattc gggatggcag
      841 aagaggtaat tccattctca atggtgatgg ttccatttgt cattgcgtta ggatatgact
      901 cgatcgtagc agtaaccgtg acttatgtcg catctcaagt cggaaatgcg acctcttgga
      961 tgagcccgtt tagcgtagcg gtggctcaag ggattgctgg aatccccgtg ttgtctggtg
     1021 cgacattccg attgattatg tgggtagttg ttactgcatt agccgctggt tatatgatga
     1081 tatatgcaga aaaaatccgt aaaaaaccag aaagttcgtt gacttacaaa tcagatgatt
     1141 atttccgttc tcatatcaaa aagacatctg atgaaaataa aaaattcatg cttgggcata
     1201 aattgatctt attagagatg ttagttgtcc ttgtctggat cgtttggggc gtgactcaaa
     1261 aaggctatta cattccagaa atcgcttctc aattctttgt gatgggatta gttgctggaa
     1321 tcattgctgt tcttttcaaa ttagatggaa tgaaagtcaa tgacatcgct tcttctttcc
     1381 aatctggtgc agctgattta gctggtacag caatcgtagt aggtatggcc aaagggattc
     1441 ttcttgtctt gggaggatct gatgcatctg ttccttcagc tttgaatacg attttgcatg
     1501 ggatcgggac ggcgttgacc ggtgttccag ctgtcattgg tgcttgggcg atgtacatct
     1561 tccaaagttt gttcaacttg gttgttacat caaattcagg acaagcagct ttgactatgc
     1621 caatcatggc tccactggca gatttagtag gtgtatccag acaagtagca gttcttgctt
     1681 atcagcttgg ttcaggtttt atggacgctt ttacacccgt atcagccagc ttgattggtg
     1741 tgctaggtgt tgcacggatc gaatgggcaa aatgggcaag attccaaatc aagatgcaag
     1801 gattcctgtt tgttttagga actattttca tcatgattgc cattgcaata ggtctgcagt
     1861 agtactggag acctgtaaat aaaaaagtaa tccaaacagt agataaggac tccaagaacc
     1921 gtcattttac ggtaaaatca aaggagtaaa gatcctatcg tttggattat tttttaagat
     1981 cctatcatca gttatactta aagaaagagg aaaaatgtat gaaaatcatt cgacaaattg
     2041 aagtgtttgc accagattac ttaggaaaaa tggatgtact gattgccggt gggaagattt
     2101 tggctgtaga agaacagcta gaaggcggct acgaaggagt ggaagtagaa gaactttcag
     2161 gtgagggaaa agtactaacc ccgggtttca ttgactgtca tttccatatt ttaggtggag
     2221 gaggagaagg cggttatcaa aatcgaacgc cagaagtcac attgagccaa ttgactacag
     2281 ctggtgtaac gacagttgtc ggttgtttgg gaacagacgg agaaggtaga gatatgactg
     2341 cgttgatcag caaagcgaaa ggtctggaag cagaaggaat ctccacttat gtatacgaag
     2401 gttcgtaccg attgccggta aaaacagtaa cagattctat catcaaagac tttttgacaa
     2461 ttgataaaat tatcggaatc ggagaaatcg cagtatccga tcaccgttct tctcagccaa
     2521 gttttgaaga gttcgctcgt gcagtagcag atgctcgtgt aggaggcatg ttatccggaa
     2581 aagctgggat catcaatgtc cacttaggcg gaggaaaaag aaagctagag ctattgacac
     2641 gaatcgtaga agaaacagaa attccgatca cacaattttt gcctactcat gcaaatcgca
     2701 caccagaatt atttgaagct tgcgtggcat ttgccaaacg tggtggcacg attgacttca
     2761 ctgctagcga agatcctgat ttttgggaga agacagatgg tgaagttcgc ttcagcaaag
     2821 cactgaaacg attgatcgag gaagatgttt ctttagataa tttcacgatg acctcagatg
     2881 gacaaggaag cttgccttat tttgatgaaa acaatcattt cctagggtta ggagtaggaa
     2941 gtgccaaggc attgctagta gggatcaaag aagctgtgca aaaagaaagt atccctttgg
     3001 aaatcgcctt acgagctata accagtaatc cagcacgtat attgaaattg gataaaaaag
     3061 gaaaaatcga aattggtgct gatgcagatc tgtgtatctt ggataaagaa acgcttgata
     3121 ttgatacagt tatcgctaaa ggagaaataa tggtccaaga aaaagaagtc aaagtatggg
     3181 ggactttcga aaaaagtttt taatcaacag ctctaactat cttttttaga aaacgagcgt
     3241 tctgtttgtc tgtatctgtt gttgatgtta ccctgaaggt agatcaataa acaaactaag
     3301 aggaggaagc acaatggcag atttaaaagg acgttctaaa gatatgaaag ataaagtagt
     3361 cggtgaagga aaagaagcat acggcaaagc aacagacgac aagagcaaag aaacagaagg
     3421 gaaagcacaa tcactaggtg ctgacatcaa agaaaaagct cgaaatttag gcgacgaagt
     3481 caaagacaag ttttctcgaa aagacgaaaa aaaataaaaa ccacttatta ctaattgaat
     3541 caagcaaata aaaaatccga accatatctg cgagacagat atggttcgga ttttttattt
     3601 gatcattatc ctgtttttta aacttcgatt ttttgaaatt ttttattttt tagttcaaag
     3661 acttcatcac agctttttgc tacttcaatc tcatgagtca cgatgataat acagcgattt
     3721 tgttcatggg cgatttcttg gaaaagtttg acgatatctt gtgaagttgt ttcatctaga
     3781 tttccagtag gctcatccgc tacgattagt tgatgttcac agcagatagc acgaacgata
     3841 gcgactcgct gctgctgacc tcccgaaagt ttggtcacct gtttttttgc taatatttca
     3901 tcaattccta cacgagccag tcgatctaaa gcaacttgtc gataattctc ttgcttggtc
     3961 ttagtgattt ccattgctgc tatgacgttg tctagagccg ataaataagg aagcaagttg
     4021 taagcttgga aaataatcga gacatcattt ttacgataac ttcttaaccc gattttgttc
     4081 agtgaacgat cttgatagta tacctttcct tcttttggtg catctagtcc agaaataagc
     4141 gaaagaaaag tcgtttttcc agaaccactt gctcctaaaa tagcgtacat ttttccagct
     4201 tcgaaattca gttctacatc tttataaagc gcatcttgcg gatcttgata ccaaaatccc
     4261 aattgttctg tccgtaacat caaattcctc ctttagttta ataggatttt tcttgggttt
     4321 aatcttaata tccctataga agctaaaaag acagaaaaca gactgattcc aaatgctact
     4381 ccggctaata gtcccaattg tgcaggtttg acattgattt ccaattcgct gacttgatca
     4441 gaaacagcaa atggattatt cgtggttcct tgttgaccat cgcgttgacc gccttgttct
     4501 tgtctgttac ctggcatttg tccaccgcca ggagcttgct ggttttgtgt cgtggtttct
     4561 gtctgttggt caagtagctg ctgtcccacg gcattcgcga cgacgtttcc gctaagcgaa
     4621 gcaactccta atgcaagaat catacaaata gccacttcgg taaacaattg gagaatgatt
     4681 ttacttctgg attctcctaa agatagcaag acgcctaact catgccgacg ctcccgaatg
     4741 gtaatcataa tgatcagagt aagtatgata ataccggcta cagccactag taaaacaaca
     4801 tttttagaaa aacttgccac gttgtttaaa ggttccaaca ttgactgata catggaatca
     4861 tttgattgta agctaaatgt atctgtgtcg atcagcttct cagcttcttt gagaaactca
     4921 tccattttat ctggatcaga tagagtgtaa atagcagaat ctatcgtgtt atcgtcagat
     4981 gtcccattta gttcatttgc aaaagtgtaa gaagtgtaaa gtgtattcga cggattcatg
     5041 aagttgaatt tcatacccat actgccgctt gtttctctgg attcataaat tcctttgatc
     5101 gtcatttcat aagtggtatc ttcatcttca gtactcgtga ttgtgaaagt atctccgact
     5161 gccaggtcat ttgtttctgc tagagtagaa tcaatgacta cattgtttgt gcctttgtcg
     5221 ctgtcatcta ttccttcacc atccgtgatt tttgctgttc catcagaaaa attactattt
     5281 tgtgcagttg ctgacacacc actgatttga aagtctgctt gagtcatttg cggattcccg
     5341 cccattcctc cagcaggttc accgcctgca tcagtagttt ctgaactgtc ttcacttgaa
     5401 tcagaactag tgatggcagt tattccatct tttgccatag ctgaggcgga actttcaaag
     5461 gaataactct ttacattatc taattcagct attttttccg cgtccgttaa ggaaactggt
     5521 gtaagagaaa aactgcctgg atctggacgt tcttcagtgg tagaaccgct tgaagaagtt
     5581 tgttcctgtt ttttaaatga agcttctcga ttggcactta aagtcactgt tgctccaact
     5641 tctttttgtg cttgattcgc tgcttcttct gcagcgcttc gaatcgtgag gccagctagc
     5701 acaaaaataa gaatggctgt gaacacggca aacaataaca atgaacgtcc ccattttact
     5761 tttgtacttt tccatgcacg tttcataaaa ttcatatttt gcatccctcc tagtagatgc
     5821 atttgataat agacaaccta tcttaaagct agcttaaatc ttatgctgtt tgcacaccat
     5881 caacatgagg ttctgtatat ataaaaagtc actatagtta ttgcaaaaaa tgtggtaaaa
     5941 tgaaaaaagt atgtttcaaa ccgacgaaga agaaatgaaa gaggaatagc aaaagaaatc
     6001 ctagatgcta cagagggatt tttacgtgca tagttgttct aacaattgct gatactagcg
     6061 atttagttta tttgttgtat aataatgggg aacagctgaa tacttatata agtattctgg
     6121 ctatagcgtt tcgcaaaaga tccattttgt taatgaagta tatccaaaat aaaatgccca
     6181 tccaactgtc agaacgcgga tgtgctggta attttcctgc tgattctcca taagttgaga
     6241 aatagtgatt ttgcatatgc ttcattggaa tggctcatat atttagataa agaagaaaat
     6301 cgacatcttt aaacttggat gtcataagaa gaggtgaagt ttgtgagtac cattaaaatt
     6361 atcccattag gcggcgttcg tgaaaatgga aaaaacatgt atgtcgcaga agtgggcgag
     6421 gatattttta ttttagattg tgggctaaaa tatcctgaaa atgagttgtt ggggatcgac
     6481 gtcgtaatcc ctgattttac gtatttagaa gaaaacattg atcgtgtagc tggtgtcttt
     6541 ttaacacatg gacatgcaga tgcgatcggg gcacttcctt acttgttgtc aaaaatttct
     6601 gtaccagtat tcgggacaga gttgacgatc gagttagcaa aattgaatgt cagtcgtaat
     6661 gaagcagcca aaggatttaa ggatttccat gtggtagatg agcatacaga aatagacttt
     6721 ggtgaaacag tagtgagctt tttccgtacc actcatacga taccagattc agtggggatc
     6781 aatctgaaaa caaaagaagg tagtatcgtt tatacgggag attttaaatt tgaccaaacg
     6841 gctatcccaa tgtatcaaac agattatgca cgattggcgg aaatcggcaa agaaggagtc
     6901 ttagccttac taagtgaatc ggcaaatgca gaaaatcctc agcctgtagc ttctgaagcc
     6961 caaatagcag atgaagtatt tgatacgatc aaatattggg aagggcgtat catcgtcgct
     7021 tgtgtagcaa gtaatttgca acgagtgcaa caagtattgg atgcagcaga aaaagcacaa
     7081 cgaaaagttg tcttgactgg tcaagatttc gaacgaatta ttcgcacagc gatgcgttta
     7141 gaaaaacttc aattaccaag tgaagatctg cttgttacgt tgaaagacat gaaaaactat
     7201 gatccagaag aactgatcat tttagaaact ggccgaatgg gtgaacccat caagtctctg
     7261 caaaaaatgg caaacggtac acaccgtaat gtcaagattg aagaaggaga tttggtttat
     7321 atcactacta cgccaagcat cgcgatggaa accatcgtgg cgaaaacgga agatatcatt
     7381 tatcgagctg gtggaacagt gaagttgatc tctgagaata tgcgcgtttc tggccatgcc
     7441 aatcctaatg acttgcaatt aatgatcaat ttgatgaaac caacatattt tgtgccagtt
     7501 caaggagagt atcgtgaatt agctgctcat gcagatttgg ctcatgcagt agggatgcca
     7561 tacaaaaata tctatattac tggacgcgga gacgtgctgg aatataagaa caagcgtatg
     7621 agtgttgcag gaactacaag tgcagaaaat gtaatgattg atggattagg cataggtgat
     7681 atcggaaaca ttgttttacg tgatcgaaaa atcttgtcag aagacgggat tttcgtagcg
     7741 gttgtcacta tcagtcggcg agaaaagaaa atcatttcaa aacctcaaat tacttcacgg
     7801 ggattcgttt acgtaaaagc tagccgtgat ctgatgaaag aaagcagcaa tatgattgaa
     7861 acaatcgttg agaaacattt agaaagcaat gattttgagt ggagcaaatt aaagcaagac
     7921 atccgagatc aattaggccg ttacttattt gaacaaacaa aacgccgccc agtgatcttg
     7981 ccagtcatta tggaagctac tcaacgtcga aataaaaaat aaacaaaaag agtaagatga
     8041 acctctctat agagtgctcg tcttgctctt tttttgcgaa aaacaaataa tttttgaata
     8101 cagcatccct cgctttataa taaacgatag aataaaggga gtgaatcaat gatgagtcaa
     8161 acaaaagtcg aacgttattt agaagaacaa aagatcagct atcaaccact tgattttagt
     8221 catgtggtag aagaatcttt cacagaagaa ctacaaaaaa gagggattga tccagctttg
     8281 atatgtaaaa ctctcgtagt gaaaggaaac aaaactgggg tgatcatcgc attgattcct
     8341 ttgaacgatc acttagatta caaaaaaacg agacaagtga cgaaagatcg taaagttggt
     8401 tttcctggaa tggattttgt attaatgcat acaggatatc cccacggagc aaacacccct
     8461 attggaataa aactcgcaca tcctgattat tgttttttag cagacagttc tttaaaagaa
     8521 aaagaagaag taattgtgtc aagcggtgaa atcgggagaa gccttcggat aaaagtagcc
     8581 gatttagaaa agatcgtcca tcctatttac gctgatttaa ttagttaagg gacgagaaga
     8641 gaagctaagc agagcagaga cctcaaactt gagattatat ctgcttggta cttggtgcat
     8701 cctgtattat ctgttacaat tgctttgtac atactgtact atcaatccaa tgaaaatata
     8761 tttagcagct atattttttc attgatgtgc aagtgatgaa atcgttttta ataaaaaaga
     8821 tacagagaaa acgcgaggtg taaaatatga aaattacgta tcatggacat tcctgtttaa
     8881 caattgaaat ggaaaatggc caacgcgtga tgattgaccc gtttattaca ggaaatccat
     8941 tgacagactt aactgcagaa gaagtaaaga cagattggtt gctgattact catggtcata
     9001 gtgaccacat aggagatatg attcctattg caaaagaaaa tcaagccaca attatcagta
     9061 ttgttgagat tgctgattat gcgaacagca gaggagtaga ttcattcggt atgaatatcg
     9121 gtggaaaaca tgcgtttcca ttcggcgtgg taaaattcgt acatgcacag catagttcaa
     9181 gctatgaagt agacggtatc gttcagtata tgggcgagcc tagcggaatt attattcaag
     9241 cagaaggaaa aacgatttat catgcagggg atactgctta tttcagtgat ctaggattgc
     9301 ttgcagaaga attcgatatt gatgtcgctt tcttgccgat tggcgataac tatacgatgg
     9361 ggccagaaga tgcagcaaag gctgcgcaaa caattcgtgc caagaaagta gttccaatcc
     9421 attacaatac tttccctgtc attgaacaag atcccgaatc ttttgtagag ttactggaag
     9481 atagtgaagg aaaaataatg agcgtctcgg aaacgctgga agtgtaggca gaaaatggcg
     9541 acaaaacatg atttgatttt agaatatatt gaaagtttac ctgttggcaa tcgaatttct
     9601 gtaagaagca tcgcaaaaga attgaatgtc agtgaaggaa cagcgtaccg tgcaatcaaa
     9661 gatgcagaaa atgtcggact agtttcaaca attcaacgag tagggaccat ccgtatcgaa
     9721 cgcaaattaa aaaaacatat tgaacgtttg acatttggcg aggtcgtcag gatcattgaa
     9781 ggggacgttt taggcggggc ggcaggatta gacaaggtct tgaataaatt cgtcatcggc
     9841 gcgatgacta aacatgccat ggaacgctat attacgccag gatcactaat gatcgtcggc
     9901 aaccgggcgg aagtccaaaa attagcattg gaagatggcg ccgcagtatt gatcacagga
     9961 ggattcaata cgactgctga gattgcaaag ctagctgatg atcttgaaat gccgatctta
    10021 cgcacctctt atgatacatt tacagtcgca accatgatca accgagcgtt aagcgaccaa
    10081 ttgatcaaaa aagatatcat gcttgtcagt gatatctata tgccagctga aaagactcat
    10141 tatttgcagc agacagatac gatttctgat tatcagcgtt tgtcagaaga aacgcagcat
    10201 tcgcgttttc cagtcgtcaa ccgccatcat cgtttgatgg ggattgttac ggcaaaagat
    10261 gttttaggga aatcgccaaa ccagatcatc gatcgggtga tgacgaaaga accaatcagt
    10321 gtaaagaaaa caatgagtat tgcctctgtt agccatcaaa tgatctggga tgggctagaa
    10381 gtcatgccgg tcgtttccga tgacttgaca ttggaaggct tgattacccg gcaagatgta
    10441 atgaaagcga tgcagcttgt ccagcgacaa ccgcagattg cagatacgat ttcggatcag
    10501 atatctggaa atatccatac gattgatact gaccgtgaag gaaatcgttt ggaaaaccct
    10561 aagttcaaat tcgtagtagc accacaaatg gtcaatggcg ttggaaccat ttcttttggc
    10621 gtacttagcg aaatcatttc tgatgtcgca caaaaaacaa tggtgatgaa ccaaaaacga
    10681 aatatcttga ttgaacaagt gaacctacat tatttgcggc tgatccaatt ggaaagcgaa
    10741 ttagacattc gtccacgtgt attggaaatc ggcagacgtt ccgctaaact ggatatcgaa
    10801 gtgtttattg aaaacaccat cgtttccaaa gcaatcgttg tttgtcaagt aatggaacgt
    10861 tcttagaaag caggagtgaa atgacagtac agcaagaaat agtagataca ataaaaaaat
    10921 acgataaaat cattatccac aggcatttgc gccctgatcc tgatgcatta ggatcacaag
    10981 taggattagc cgaactatta cgggaaagtt tcccagaaaa agaaatacgt caagtaggtg
    11041 aaacgattga agggcttcgc tttttagcag aaatgcaaga agttgaagat gtcttctacc
    11101 aaggagcgct agtcatcgtg actgatacag ctaattcacc cagaatcagt gatcaaagat
    11161 atcagttggc agaaaaactg atcaaaatcg accatcatcc caatgatgac ccttatggag
    11221 atcttgtgtg ggtaaataca aaagcaagta gctgtagtga gatgattgca gaatttgctt
    11281 tgatgtttcc tgaatcttta aagatgaacg ttaaggctgc acggttactt tatgcaggaa
    11341 tcgtcggaga tacagggcgc tttttgtatc cctccacgac gtcccgtaca ttagagattg
    11401 cttcgatatt gagaaattat ccatttgatg caagtgcttt gaacagagag attgaacaaa
    11461 tgccgatgaa agtcgctaaa ctttccggtt atctttatca aaatatccag gtggatgaaa
    11521 atggtgcagg aagaataatc cttccgcaag aaattcttga taaatatgga atcgatgatt
    11581 cagagacagc ttcgatcgtt tcacttcctg gagtgattga cgaagtgctg gcttggggga
    11641 tttttgttca gcagccttca gggtattacc gcgtacgttt acgttctaag gggccgatca
    11701 tcaatgaatt ggctaaacgc catcatggag gaggccatcc attagcaagt ggtgcgaatg
    11761 caaaagattt agcagaagta gaggaaatct ataaagagat ccaagatata tgccaatcgt
    11821 ataagagcag aaaaacagaa tgatacttcg ttcgaaaacg tgccttttgt agtaaaaatc
    11881 acaaatgtat cttttcaaga tcttcttttt ccgttacaat agtgctcact aaagatttta
    11941 aaggagaaag aagatgacat taccaaattg cccagaatgt ggctcagagt acacatatga
    12001 agatcgaggc ttgttgattt gcccagaatg cgggaacgaa tggaacaaag aagaacaaga
    12061 aactgtcata gaagacggat tagtggtatt agatgcaaat ggaaaccgtc tgttagaggg
    12121 cgatagcgtg acagtgatta aagacttgaa agtaaaaggt gcgtcaggtg ccattaaaca
    12181 aggaacaaaa gtcaagaaca tccgtttagt agaaggcgat cataatatcg attgcaaggt
    12241 agaaggattt ggaccgatga aactgaaatc ggaatttgtg aaaaagaatt aaagaatatt
    12301 caaggtcttt tcacattctt ttcctactac taatgataaa ataaagtaaa cgaaaaggag
    12361 tgaaacgaaa atgtatggtt atggaatgta tggtttttta gatccgacat ttcttttggt
    12421 cattcttgga ttagttattt ccggaattgc gtcagcgtac gtcaatagta ctttccgaaa
    12481 atacgatcaa gtgagaagca aaaacaatgt gactggtaca caagcagcac aatatatatt
    12541 acaaagtcaa cagataaata atgtcggtgt tcaacaaatt gccggagatc tcacagataa
    12601 ctacaattca ggtaataaga tgttgagttt gtctcaagca actgcacagt ctacttcagt
    12661 tgctgcgata ggtgtagcag ctcatgaatg cggacatgcg gtacaagatg cttcgggtta
    12721 tgtcccattg aagattcgtg cagcacttgt tccagcagct aactttggtt cgatgatttc
    12781 gttccccttg attatggtag gtgtattgct tagctggaac tctacgttaa tcaatatcgg
    12841 gattttcgct ttttctttag cattactttt ccaattggtg acattaccag tcgaatttaa
    12901 cgcgtctcgg cgagcaatcc aaattttgag tgaaggcgga ctactgacgg aagaagaagt
    12961 accgatggca agacacgtat tatttgcagc agcactgact tatgttgcag cagcgctttc
    13021 gacattcctg cagctgttgc gcttgatctt attgttcggc ggcaaccgaa gagattaaaa
    13081 taaagaagtt gtgaaatcag cgctttgatc tgagctaata ccgagagtta gctgattgaa
    13141 taccatttat ttggaa
//
LOCUS       DAYYBG010000071        13155 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000071 DAYYBG010000000
VERSION     DAYYBG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.072"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..795)
                     /locus_tag="ACHAUN_002026"
     CDS             complement(<1..795)
                     /locus_tag="ACHAUN_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HFM8460711.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYK"
     gene            complement(792..1445)
                     /locus_tag="ACHAUN_002027"
     CDS             complement(792..1445)
                     /locus_tag="ACHAUN_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8460712.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1733..2253
                     /locus_tag="ACHAUN_002028"
                     /pseudo
     CDS             1733..2253
                     /locus_tag="ACHAUN_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2251..2970)
                     /locus_tag="ACHAUN_002029"
                     /pseudo
     CDS             complement(2251..2970)
                     /locus_tag="ACHAUN_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2995..3180)
                     /locus_tag="ACHAUN_002030"
     CDS             complement(2995..3180)
                     /locus_tag="ACHAUN_002030"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8460713.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3407..4680
                     /locus_tag="ACHAUN_002031"
                     /pseudo
     CDS             3407..4680
                     /locus_tag="ACHAUN_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4787..5818)
                     /locus_tag="ACHAUN_002032"
     CDS             complement(4787..5818)
                     /locus_tag="ACHAUN_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8460714.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5825..6655)
                     /locus_tag="ACHAUN_002033"
     CDS             complement(5825..6655)
                     /locus_tag="ACHAUN_002033"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8460715.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6652..7467)
                     /locus_tag="ACHAUN_002034"
     CDS             complement(6652..7467)
                     /locus_tag="ACHAUN_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8460716.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7464..8288)
                     /locus_tag="ACHAUN_002035"
     CDS             complement(7464..8288)
                     /locus_tag="ACHAUN_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFM8460717.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8278..9378)
                     /locus_tag="ACHAUN_002036"
     CDS             complement(8278..9378)
                     /locus_tag="ACHAUN_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8460718.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9556..10341
                     /locus_tag="ACHAUN_002037"
     CDS             9556..10341
                     /locus_tag="ACHAUN_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8460719.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10374..10757
                     /locus_tag="ACHAUN_002038"
     CDS             10374..10757
                     /locus_tag="ACHAUN_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8460720.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10833..10964
                     /locus_tag="ACHAUN_002039"
     CDS             10833..10964
                     /locus_tag="ACHAUN_002039"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8460721.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10933..11169
                     /locus_tag="ACHAUN_002040"
     CDS             10933..11169
                     /locus_tag="ACHAUN_002040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8460722.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11181..11267
                     /locus_tag="ACHAUN_002041"
                     /pseudo
     CDS             11181..11267
                     /locus_tag="ACHAUN_002041"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11247..12218)
                     /locus_tag="ACHAUN_002042"
     CDS             complement(11247..12218)
                     /locus_tag="ACHAUN_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8460723.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12870..>13155
                     /locus_tag="ACHAUN_002043"
     CDS             12870..>13155
                     /locus_tag="ACHAUN_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8460724.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3959 a         2900 c         2348 g         3948 t
ORIGIN      
        1 tttatatttt ccaatattgt atttcatgtt ttccagtatt attttaagac tagcaagagg
       61 ggttttaagt tcatgggatg cacctttaaa aaagtcatat tttaactttt ctaattttaa
      121 aatttcttta tttttaaatt ccaaatcgtc aattgttctt aataaagtag aatacaaatc
      181 attaatttgt tgttttaatt ctccaacttc atcattagag ctaactttta aactcatttt
      241 tttatcaagt ttcatcattt tatcagtaac aatttttatt tcttgtatat tattttttat
      301 tgattttgca taaattaaag aaataatagc agaaaataaa attgatatta ataaagaata
      361 tggtaaaaat ttaaggctta aatctttcgc atctttttgc atatcagctg tagaaacaaa
      421 ttgtagattt gtttttttac catcattaag ctttatttct ctttcttcaa ttatcaaaga
      481 attactatca ctttctaaat taacatttat attatctttg atttgtattt cattcttatt
      541 atttttttct tttatgaata cttttatttc actactcttg gaataaaact ccaaagtttg
      601 ttctatatat tttatttctt ttccattcat attactagaa atttcatttg ccatattatg
      661 aatcttttct tttctcgtct ccaaatatgt tttaggaaag ataaaaaaaa ttaatgaatg
      721 aactaatata attattattc caagaacaga aaatatttgt atgaacattt ttggaaatat
      781 ctttaatttt ttcattttct ctccaattta tatcctacat ttcttatagt agttatacag
      841 tctaattgta atttttttct aagttctttt atatatacat ctattactcg atcataagga
      901 gtttcttcgc tatctttcca tacataatca ataatttgca ttcttgttaa cacttgtcca
      961 ttattatcca ataaacattt taatacttct agttcttttg catttacatc tatttcttca
     1021 ccatttattt tagctgtata gctattaaaa ttaaccgata gatccttata ttcaaacttc
     1081 tctaaatgtc cataattctt cttaattaaa gaatctattc tagcttttaa gaccggcaat
     1141 gaaaatggct tttctacata tccgtctact aaattagtaa atgcatcaat tttatattct
     1201 tcatcactaa atgcagtcaa gattagaatt ggtaaattgc ttttctttct aatttctttc
     1261 aacacttcta aaccatttat aaaaggtatc tgaatgtcta agataaccaa atttatatcg
     1321 ctattaaatt ttgacaaggc ttctcttcca tctttagctt gaataacagt atatccaaat
     1381 tctgaaagat attcacttat tccctctctt atcatattat catcttcaac agttaatatt
     1441 ttcatatata cctccactta agcctataca ctagatatta tacagcatat tatggctcta
     1501 taatatctag tgttggtgac gccttctgtt gccaatactt tttctttttt ttaataaatg
     1561 caaacagaca ccttgaaatc ctttagaaaa aaggcttcta taatattttg tgttgttgga
     1621 aaatcgaagg cgattttcta ctaacacttt ttcttcgttt caactcaaaa agagactcac
     1681 ttttggtaaa atttagtcga ccaaaacatc accctaccaa aggagtcgct ctatggataa
     1741 tcatactaga aaattactcg gattaacaga taaacattta ttttttgatg aagaatggct
     1801 gattgaaaaa gaatataaag gcgttcaagc gcagtttatt aaagggagat tggatgactc
     1861 accttcccat tgtgaacact gtggatcgat tcgtattatc cgcaacggtt cctatacgac
     1921 acgtactcag atgttgaaag tcaaagaaaa gttgactatt ttagaattaa aacgtacccg
     1981 ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa acggacttag ttgatgaaca
     2041 tcatcaacta acaaaggaac tgaagcaggc gattctcata gaactgtacg aaaatcaatc
     2101 acgtaatctg aaaatcagaa gaagtaccgt cggctcaagc gatattggaa actcttactc
     2161 aaagattcca cgacattgga gccgctcaaa cggcattatc atcgactgtt taaacgacca
     2221 attagtcaaa cagaaatcgt cgatgaaccg ttgtgcacaa tggtgtcctg aacgtacagc
     2281 gacaccttcc atatccagag cagtcgccac atcacgggga tggatgccat ccaaattaaa
     2341 agcaaccacg ccagtatgat gttcaggatc ttgcggtccg taaatggtga taccgccgat
     2401 cgcttgtaat ttaggtaaaa cgtaggcaac cagttcttgc tcataacgat gaatattctc
     2461 cataccaatc tcattcaagt aatcgattgc tcctgcaatg ttcggagtac cagcttcaaa
     2521 tttccatggc agctctttcc atgaactatc gtaaagattg acaaaatcta tcatttcacc
     2581 gccgaattcg actggctcca tttgttctaa caattgacgt ttgccatata atacaccgat
     2641 tcctgtcggc ccgcacattt tatgcccgct aaaggcgaaa aaatctgcat ccaacgcttg
     2701 tacatcaact gtcatatgcg gtgctgcttg ggcaccgact ggactatcct caactacaac
     2761 agcctgttca accctcagcc gtttcaagca caacaaataa ccttctggat ctggtttgcc
     2821 gacctgaaga tcatcgatcg tcacaaagga atcaattttt tccttaatca tcggatgtgc
     2881 ttcaaaaaaa tagtctagct cccgcagttc agaattgaga ctactccgat tttccagtgc
     2941 tgctgatccg ctgctcaaaa tgttcagcat ccactggtct cccaaccgct tgacttatcc
     3001 cttcataaaa ggacagttct gctgccgtcc caacaccttg ttgatagtct gcaaaaggaa
     3061 ttttatagcc gaactgctcc agtaaatagg cttcagtaaa tagtgatgaa tactctcgta
     3121 aaacatccat tcactgtcaa tcatcgtccc atcaaaatca aaaataagtg ctttggtcat
     3181 cttctttccc ccttacccaa aacaagcaaa agctgtgtcc tgctctctct acagtatcag
     3241 cataaaaact gccgtctgaa aagtcaaagg tgaaagaaat gtggcgctat aatttctagg
     3301 actgatagta aatcattcta tcagtccttt tgagcataaa aaaacaaaac atacagaagc
     3361 tccgttagaa ttgaatcacc acaacacaat caaaggagcg ttctgtatgc ctgatcttca
     3421 tcttatcaaa ctattactag gaatcaaaga tggaaacatt caaatcactc atgtcgagaa
     3481 gaaaaaaata cattcaatca acagtatcgt tgtttctgcc acgctcatta aaacgattca
     3541 gccctgtcct cagtgtaaac aggtgaatca acatgggctg atcgttaaga acggaaagaa
     3601 aaaatcgctg gtccttctca ataagtgtgc gaatcaacgg acttatcttg ctttggcaaa
     3661 gcaacggtac ctctgtcgtg gctgtcacac ctattttacg gcgaatacct acattgttga
     3721 aaaaaactgt tttatcgcca aacaagtaca ttataaaata ttggaggaat tgactgaaaa
     3781 acaagcaatg acgacgattt ctaaacattg cggcgtttct tggtcaaccg tttcgcgaac
     3841 actcgcttct ctgctcccca tgacaaacgt gaaaataaat tggttgcctc gctgttcatt
     3901 ggtagatgaa tttcgttcat tgaaaaatca agtcggtccc tattcttttt cttgtatgga
     3961 tggcgatact gggaaattac tggatattct tccttcacgt aagaagaaag atctcatttc
     4021 ttattttatg caatttgaac gacgtgcacg tttaaacgta aaaatactcg taacagatat
     4081 gaacgcctct tatgctttgc tgataaaaga gtgctttcct catgccaaac tcgttgtgga
     4141 tcgctttcac atcgtcaagc acctcattcg cagttttaag gatatttgac tatgagtcat
     4201 gaaaagcttc gaccgaaacg atccaataca agccaaacac tatcgacaag tgaaggcttt
     4261 atctcgactt ctgatcaaac gacaggacac gcttgtttat gataaatgga caaaatggcg
     4321 taattttggt tgggcctatt taaccgaaag tgaagtcgta gaacgtctac tttctacttc
     4381 tgatgaacta cgaatagctt atgcttacta tcaagaaatt cttcaagctt tttatgacaa
     4441 agaagcggac atcttctttc agcttgtaaa aacaatgccc aagtcggttc caagagaact
     4501 gcatcatata aaaaaagcgt ttataaacta tgaatccggc attcgtttag ccttagagtt
     4561 gccttactca aatggcaaaa tagaaaatct gcatacccac atcaaagcat tgaaacgaat
     4621 agcttacggg ttaggatgag aaccagaatt ttcttaatca atcatctgat cacttattaa
     4681 acataaaaaa acatctaaag cagaaaatca attctacctt agatgttctt atatcactct
     4741 atcagtccta tttgacaaag agccagaaat gtttgccgac tatacactag ctttgttgtg
     4801 caaaatgctg agtatcaatt tccacaggga ttgctagaat atctttcagc tgaatgtata
     4861 aggcatcgct aattttctct tcccgatcgt tcaaccgatc cactaaaatt tctttgccct
     4921 ccagcgtttg aaaaacatac cgttgaatgt tgcctaaaat aaatgattcc ttccatagaa
     4981 ccggaatctt aaccccctga ggaaccggtt caaattgaat cagctccggt cttaaataat
     5041 aaatgcagcg attgccctta gcggaaaact cgtgggtata ctgattcagc tcataccatt
     5101 cgaaacgatt atagttgccg ataaattccg caacaaatcg gctctgagga ttctgataca
     5161 attccttcga ggtggaaacc tgagccaccc gcccagcttc catcacaaaa atccgatcac
     5221 tgattcgcat cgcctcttct tggtcatgag tcacaaaaat catcgtcatc cctagcgatt
     5281 gctggatagc gcgcaaatcc cgctgcagct gttttctaat tcgggcatcc aaagcactta
     5341 atggctcatc cagcagcagg actttcggtt cagtgaccaa tgagcgagcc agtgccaccc
     5401 gttgtcgttg accgccagaa agaaactgga catttttatc tgcctgctcc gtcatatcca
     5461 ccaatgatag catctcaaaa accttttcct gaatcacctc aggactcagc ttctttacct
     5521 tcaaaccaaa ggctacattt tcaaaaacag tcatagttgg aaacaatgca tactgttgaa
     5581 aaacaaagcc gatattgcga tccttcgtag gcttatcggc aatattctca ttgtccagca
     5641 caatctcacc gctctttggt tgttccaatc ccgtgataca gcgcagcaac gtagactttc
     5701 cgcacccgct gggacctagt aaagtaacca gctcaccctt ttcaatctga atttcatcaa
     5761 tagctaaaat ctgtttttca tcaaaaaaca gattgagatc atgtatttct aaaaacattt
     5821 tccgtcacaa ctcctttaag ggcttctttt tcgactgctt ctcttccctc gtctgcttaa
     5881 gggacagctg ttgcttttgt cgataagtca gcataaaggt agttaaagca attcccacca
     5941 taaacagcaa atacaaaacc atcacagcgc tagccaaatg gccgttttcg ttcatccgcc
     6001 gcagcatata gatgcgtacc gtctgaaatc gaccgccaat caacaggttc gtcagcatgt
     6061 actccccaaa aaccgaagaa aaaatcatca gagaaactag ggtcacaccg atacggatgt
     6121 ttggaaacag cacctggata ataatcgtag acatcggggc gcccaaggtt tctgctgcat
     6181 ccactaattc cttcagatta attaagcgca tctggttgtt aagactaaga tagacaatcg
     6241 gcaacgccgt aataaacaat gcacctacca acactacaaa cattgggata ccagtttcag
     6301 catacgttcg aagcaaagcc gttaccaaaa tcactcctgg taaagcatac ggcagcagga
     6361 gcagcttttc cagccaacga ttcaaacgag ggaaatacaa atgaatccaa ataatggtgg
     6421 gaatcatcaa caacagaatc gtcaccaaga ccacacctgc taataaaagt gagcgaccca
     6481 ccgcagcaat aaattccctg tccgtcagca gctgttggaa ccattgcaag gtaaaatcac
     6541 taggtaaaag cgattttgac catttagtgg agctggcata caaaatcgtt ccaataatcg
     6601 gcagcaagta atacaggaca aagcagaaca aaatcggttt tattaacttt ttcacgtctg
     6661 caccaccttg cctttctttt gaataatcaa cattgccgtt accagcagga cacctaaagc
     6721 gactgccaac gcactgccta aattaggctt ggcgaacaca tctcccgaca ctaaagccgc
     6781 aattcgaatc gttaaaagat ttacattact gctggtcagg gcatacgctg tttcataggt
     6841 ccccatgcca ttagcaaata agatgacaaa ggtgctggca atcgatggtc gcaaaaaagg
     6901 caaagccacc ttgaaccaag catacgctga tgaagcgccc agcaattgtg cggactccag
     6961 ccattccttt ttcacttcct tcatgctggg atacaaaaac aagaccccta acggaatttg
     7021 gaaataggta tacgtgatgg tcaatcccca ccaagaatac aaattgaagt ccgctagaaa
     7081 tggcaaaatc attttcagca cacctacatt ccccaacaaa ataataaaag agaatgccag
     7141 cggaatccca gcaaaattag ctgccaaatt ggaaacaaag gtgattttct cctgcaattt
     7201 agtcgataga ttcattaagc ataaacataa aataaacgtc ccgattaacc caatcacact
     7261 ggagctgaca gcaatcagta cgctgttaac aaaggcctgt gtgtaatatt tgttgctgaa
     7321 aatatcactg taattggcca atgtccactg gttagttaaa tcactgtgca agctgcacag
     7381 aaccataaaa agcagcggca gcagtaaaaa tgcacagaag ataaaggagg caggaaccag
     7441 aaccaagcgt tttttcatct ctttcatccc ctcatcaatt cttgaatgtc ctgcattttc
     7501 tctgacggtg caattcctaa taaccagcaa aacaatggag ccgccagcag ctgtggtaca
     7561 gtttgttcct tcagcttttg gcgtttattt gaaatcaaat aaaaaggaac ttcacgatgt
     7621 gcagccaagc tgccgccatg cagtccgtgc gcatccatcc cgtgatccgc agtcaccacg
     7681 acttgataac ctttttgcag ccattcagga aggtaaatag ccaataatct gtccgcctga
     7741 ttaaccgctg ctgcatattc atgagaatcg gcggtaaatt tgtgaccgac atcatcaata
     7801 ttcatcgaat gtacgaccaa aaagtctggc tgatactgat tcactaaata atgggcatcc
     7861 gcaaataaat gcgaatcagg gtaagtgtcc tcactataaa agatgccatg ctgaatcagt
     7921 cgctcctcct gatgctggat gcgatcaacc aaacgattat agggggcttg attgtacaac
     7981 tcgctgtacc agtggtaagc ggcggctcct gtttttccgc cagcttcctt aactaattca
     8041 aacagcgacc actctttgga tcgctgagca tggtaattac tatagatgcc attttcatag
     8101 gctggaacgc cggtcatcag tacttcgtat aacggacgtg aattgctggg cagctcactt
     8161 tttaccttca ataagcttgc ttgctggtgc tcaactaaat gattcatgaa cccaagctgg
     8221 gcgacggctg tgtcgtagcg acagccgtct agtataacta caatcagttt gtcaagtcta
     8281 ctttgcatag gtcagtacct ccgcctgcca aagctctgga agctctacag aggtcttgtt
     8341 ccaagcttca ttgtcttcaa ctggcaccgc cgcttcataa gcatcctccg gcagtaattt
     8401 ttcagctact tctttaggca gctctacctt gcgaataggt cgcgcatagc cctttgccaa
     8461 attgatttgc ccttcatcag ataaaatata ttcccgtgct agctttgccg cattgggatg
     8521 aggggcattt ttattgatta aggttgtata cccgctaatg atggagccgt ctgttggcac
     8581 agttacctca aaacgctcgg agtcaatttg gtcacgataa tttagtccat tgaagtccca
     8641 aacaactgcc acatcgattt cacctttttc aagatttgct acgctggcat caacggtgga
     8701 caaacggcct ttttctgcca tttttttgta gtaattcaga cctggctgaa tatccttttc
     8761 gtcacctccg ttggcaatcg ccgcagccaa tacagcaaat tgtgcttggt tggcaatcgt
     8821 cacatccccc acgctaactt gatagtcacc ctcttgcagc tccttccagc tagtcggtgc
     8881 ttgcttcaca ttctctttat cggtgataaa ggaaatggtt cccgtatagc ttaacaacca
     8941 atccccatca tcatcctttg cccaatccgg aatttcctcc caatagttgg tcttataagg
     9001 taaggttaac tctttgcttt cagccaacgg accgaagctg ataccgacat caccaatatc
     9061 ggcggttcca ttttttcctt ctgattcaaa cttagccaat tcttctgcac tggacatatc
     9121 cgtatccacg tgacttaagc cgtattcttt ttcaaggtcg ttccaagtgc ctacccaatt
     9181 tgcccaagta tccggcattc ccaagctggc aatctctcct tcttccttgg ctttttctat
     9241 aatcgtatct aaagagtcgt ctgccgcagc cgattgcttc gttgcttcac ctgagccgca
     9301 gcctgtcagc aggatactta ctgttgctgc acaaaataaa ccgatagtca tgttacgaat
     9361 tttttcattc attttcattc attcttctcc cctttcgata attccattct aacaaagctt
     9421 tgtaatgaga gaatcagttt cttgaaaagg tttcatgaac aatgtaaagg ttgtatatgg
     9481 tttgtaaatt tcgacgaatt cccgttaacc ttctaaagct tttgcggtat gatagtagaa
     9541 aaaaggaggc tccctatgca gtattatgat cagcatctcc acacgtattt ttcctttgat
     9601 tcagaggaaa aatttgaaaa ctatttagcc tatcagccgg agtttttcgt ttctacggat
     9661 cactttgatt taaacaatcc atctaccgga aaagatgaca ttccggatta tccagcctat
     9721 ataaacaaac tggactcttt aaaagaaacc tattccacca aatttctgcg aggtatcgag
     9781 attggcgttg ttccaggaca agaagagcgg atcgtggatt acctcgctca gcatccttat
     9841 gatttaaagc tggtaagtat tcatcaaaat ggccgctttg attatatgga cgatgtggtt
     9901 ttaaccaaag atcccattca aactaccaag gaatattttg atcagatgtt gcaggtcttg
     9961 acggattttc cagaaggaga tattttgact cactttgatt atggattacg ccgcttcacc
    10021 ttcacagcgg aagaattcgc ggagcatttt gaggctcagc tgctaacgat tttcaaaaag
    10081 gttgttgaac gagaattggc gatggagtta aatgccaaga gcttcttgaa atacggcaac
    10141 gaagaccttt accgttatgc cgttcctttg tatcaaagtg tcggcggaaa gctctttacc
    10201 ttaggctcgg atgctcatgt tgccgaagat tatcagctgg gctttaaaaa aatgagtcag
    10261 ctgttggcag actgtcaggt agaaaagttg ctagtcattc aaggaaaaga acgccgcctg
    10321 acgccgctgc cgattttgtg attcctatat aatagaagaa agtggtgaaa aaaatgagca
    10381 acctgtggga ctacaatcaa gaggcaccca tccattattt aattgcgcga cattgggatg
    10441 cgttgaaaat tgaggcggtt tgccgatcct tgttagcagc cgttccaaaa caacaactgg
    10501 aaaatttttt ggtggctgac agcttgcagc gggaaaaagt tcaggcttat tttgcagcct
    10561 ttaaagatca gcctttggaa tatttgcatg cgcagtttca tcttttttat caagtagccg
    10621 ccccagatga ctacaacgat ttacgaggac agctgcaatt gacctttcaa gcggatgaaa
    10681 ccgcctatac cgttttattg ggaatggctc gccttggtga tcaggcgaag gtcgagtggc
    10741 ggattttcga tatttgatgg caggatcaag gcgtttcagt tgcaaacctt cctctctttc
    10801 aattagaaaa accgcagcag gaagaagttt gtgtgctggt caccgatgct ggtgaaattc
    10861 gtctccgctt atttcctgac caagctcccc tggctgtagc caactggcgc tccctagctg
    10921 agcaaggcgt ttatgacaac acacctttcg gctcgtgtca ttaaggaatt tgttattcaa
    10981 ggtggcgctt tggatggttc tggtgatgaa gctgtttcca gctatgaggg ctttttcgct
    11041 gatgaagtgc atcgcggact ttatcatttt aatggcgcct tggccttagg ggaccacggc
    11101 ccccacacca acggcaacca gttttttatt gtgcaaaaca caaaggcgca ggctgacctg
    11161 ctaatgtaaa gcaagcatac ttggagcttg gaggactacc ggagctggat ggtcgttata
    11221 ctgtttttgg acaggtatac caagggttag aaatcgtagc agcgatcgca gctcagccga
    11281 caaacagtga agatgtaccg attgagccag tgttcattcg acaaattaac ttcgaaaaaa
    11341 catcgtaaaa agacactcac tgacttcagc aaaagctcta ttcttgagct ggctgcagct
    11401 agtgagtgtc tttcatttat ccataagata aagcttggat tcaataaata aataaatgtt
    11461 ttggttaaag ctgtgaccag caaatgagga accgtgcgag ccagttgatc tgccaatgca
    11521 aacatttctt taggtgaagg cgctggcgtg tctgccaacg gatactccag tcccaatgct
    11581 tggtaaccgt gcataccata gcagtggtac ggtagtaatt ctaacggtac gaaccgatca
    11641 agatctgcca caaaacaagc aatcgtcagc agctcggtca ggttcatatt gatacctgga
    11701 accacaggaa ctcgaattcg cacttcgcag ttaggatact caattaaaaa gcgacgaata
    11761 ttcttcaaaa tttgttggtt atctaagccg gtccattgtt tgtggagctt gctggtaaac
    11821 atttttacat ccacaaacaa tgtatccaca tagggagcta ccttttggat ttcattataa
    11881 gcaccacaaa agctggtttc cagtacggta ttgattccaa tgtatttcga tttttgcaaa
    11941 atttctttag caaaatccgc ctgaaccagt gcctcgcctc cgctaatggt cactccgcca
    12001 tccgagtgaa agtaaaaaac agcatccttc tcaatctcat ccattacctc ttcagcggtc
    12061 atggtctggc caaatccttt ttcaatctgc ttgctttgtg attcgggaat cgaacaccag
    12121 gcacagcgta atgggcagcc ctttaaataa acaacggtcc gtaaaccagc accatcatgt
    12181 agagaaccct gttctattct taatactgtt ccttccatat agatttttga attacacact
    12241 agtttaaact aaaacacaca aggaaaaact taccatttaa tcataaaatt agctcatata
    12301 aatatactag gatgtaaaat agactggtca aaatctggtc aaaaaagagg actaatacag
    12361 gtagtcctct tttttgttat tttaatattc taaatcattt tttattggtt tattccctta
    12421 ttcgatgtag actcaaatca ctatatcact agatatcaaa cggcgtcata cggaatcaaa
    12481 aacatcaaat ttgacctgtt aaatggtcat tgaataacat tcaaaatcaa taaaacggag
    12541 tcagacacgg agtcaaaaac gctgagagta aaaaatatat acagccttgt aggtaaagta
    12601 ccacagggct gtatattttt tatttcagat taccttaaat gattccattt actttttatc
    12661 tcattttttc ctactagtta gtatcaaata ctctcctatt ttctcaataa actatttttg
    12721 gatacatggt atctgaaaca gctaaaacct tgtcattaca gcaaaaaatc acgttatcca
    12781 gactgtagac aaagtccatt ttatggatga tgtctacagt cttttctttt ataatagatt
    12841 tatgaaaacg aatgcaagga gtgatagcta tgatgtctaa aaacaatacg aatggtagaa
    12901 atcaatttgc gatgcttacg attgatgatc tagttcctca agatcatctc gttcgaaaga
    12961 ttgatgccgc gcttgatttt gaatttattt atccaatcgt cgaagccact tactccgatt
    13021 taggtcgccc aagtatcgat ccagtgattc tgatcaaatt ggtgtttatt cagtatttat
    13081 tcggtatccg ttccatgcga caaaccatta aggaagtcga taccaatgtc gcttaccgct
    13141 ggtttttagg ctact
//
LOCUS       DAYYBG010000072        12747 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000072 DAYYBG010000000
VERSION     DAYYBG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.073"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHAUN_002044"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHAUN_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8460725.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACHAUN_002045"
     CDS             complement(323..892)
                     /locus_tag="ACHAUN_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8460726.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACHAUN_002046"
     CDS             1078..1980
                     /locus_tag="ACHAUN_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8460727.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHAUN_002047"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHAUN_002047"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8460728.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHAUN_002048"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHAUN_002048"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8460729.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACHAUN_002049"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACHAUN_002049"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8460730.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACHAUN_002050"
     CDS             8554..8739
                     /locus_tag="ACHAUN_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8460731.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACHAUN_002051"
     CDS             complement(8801..9997)
                     /locus_tag="ACHAUN_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8460732.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACHAUN_002052"
     CDS             complement(10167..11603)
                     /locus_tag="ACHAUN_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8460733.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACHAUN_002053"
     CDS             11896..12705
                     /locus_tag="ACHAUN_002053"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8460734.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAYYBG010000073        12742 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000073 DAYYBG010000000
VERSION     DAYYBG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.074"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..544
                     /locus_tag="ACHAUN_002054"
     CDS             101..544
                     /locus_tag="ACHAUN_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8460735.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="ACHAUN_002055"
     CDS             complement(550..1734)
                     /locus_tag="ACHAUN_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8460736.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="ACHAUN_002056"
     CDS             2002..2700
                     /locus_tag="ACHAUN_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8460737.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="ACHAUN_002057"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="ACHAUN_002057"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8460738.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="ACHAUN_002058"
     CDS             complement(3510..4043)
                     /locus_tag="ACHAUN_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460739.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="ACHAUN_002059"
     CDS             complement(4340..6721)
                     /locus_tag="ACHAUN_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8460740.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="ACHAUN_002060"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="ACHAUN_002060"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8460741.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="ACHAUN_002061"
     CDS             7422..7961
                     /locus_tag="ACHAUN_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8460742.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="ACHAUN_002062"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="ACHAUN_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8460743.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="ACHAUN_002063"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHAUN_002063"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="ACHAUN_002064"
     CDS             9068..10234
                     /locus_tag="ACHAUN_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8460744.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="ACHAUN_002065"
     CDS             10280..11776
                     /locus_tag="ACHAUN_002065"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8460745.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="ACHAUN_002066"
     CDS             11857..12540
                     /locus_tag="ACHAUN_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8460746.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12549..>12742
                     /locus_tag="ACHAUN_002067"
     CDS             12549..>12742
                     /locus_tag="ACHAUN_002067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460747.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4273 a         2230 c         2630 g         3609 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggc gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta tt
//
LOCUS       DAYYBG010000074        12506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000074 DAYYBG010000000
VERSION     DAYYBG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.075"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..327)
                     /locus_tag="ACHAUN_002068"
     CDS             complement(<1..327)
                     /locus_tag="ACHAUN_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8460748.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCA"
     gene            complement(354..944)
                     /locus_tag="ACHAUN_002069"
     CDS             complement(354..944)
                     /locus_tag="ACHAUN_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFM8460749.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(1262..2323)
                     /locus_tag="ACHAUN_002070"
     CDS             complement(1262..2323)
                     /locus_tag="ACHAUN_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8460750.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(2891..3958)
                     /locus_tag="ACHAUN_002071"
     CDS             complement(2891..3958)
                     /locus_tag="ACHAUN_002071"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8460751.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(3978..4457)
                     /gene="pssE"
                     /locus_tag="ACHAUN_002072"
     CDS             complement(3978..4457)
                     /gene="pssE"
                     /locus_tag="ACHAUN_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFM8460752.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(4474..4935)
                     /gene="pssD"
                     /locus_tag="ACHAUN_002073"
     CDS             complement(4474..4935)
                     /gene="pssD"
                     /locus_tag="ACHAUN_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFM8460753.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(4949..6199)
                     /locus_tag="ACHAUN_002074"
     CDS             complement(4949..6199)
                     /locus_tag="ACHAUN_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8460754.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(6577..8028)
                     /locus_tag="ACHAUN_002075"
     CDS             complement(6577..8028)
                     /locus_tag="ACHAUN_002075"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8460755.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(8349..9113)
                     /locus_tag="ACHAUN_002076"
     CDS             complement(8349..9113)
                     /locus_tag="ACHAUN_002076"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8460756.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(9141..9839)
                     /locus_tag="ACHAUN_002077"
     CDS             complement(9141..9839)
                     /locus_tag="ACHAUN_002077"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8460757.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(9851..10630)
                     /locus_tag="ACHAUN_002078"
     CDS             complement(9851..10630)
                     /locus_tag="ACHAUN_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8460758.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(10646..11590)
                     /locus_tag="ACHAUN_002079"
     CDS             complement(10646..11590)
                     /locus_tag="ACHAUN_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8460759.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(11636..12415)
                     /locus_tag="ACHAUN_002080"
     CDS             complement(11636..12415)
                     /locus_tag="ACHAUN_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460760.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         3724 a         2557 c         1785 g         4440 t
ORIGIN      
        1 tgcacaattt atatatacta tgaacatacg agtgttattt cgtatccccc agataatcaa
       61 tcctatattt tctaaattaa taactgagct acaaatagaa actagtaaaa acaaaactat
      121 cagcatctca tttttgctag catatattcc ttttctgcgt ctaaagctaa gtgcatttaa
      181 taacacaaag atattaatta tatctcccaa atataaaatt gctcttgggg cgttaaaagt
      241 atatacaaga actatggaga ttgcaaaaaa tgccaacgtg aacaatgtcc aatctgctcc
      301 tgaccatctt tttattgaaa tacgcatcgt tattcacctt ctttcgctaa aaattattct
      361 aaaaatactt ttgttaaatt aacataccct attatttttg ccggtacacc tgcaagaatt
      421 gcaccattct ctagacaatc tttgataaca atagctcctg ccgcaacaac tacattgtta
      481 gctattttta cattaccaat aatctttgca cccacgcata cacgtacatt atttccaatt
      541 acaggacact taggatcgat tccatcattt ccaatacagt tattcccata taagtaacaa
      601 ttttttccta ttatggcgtt accattaata acaattcctt gtgtatggta aaatcttaca
      661 ccttcatcaa atacattttc accacactct ataccacatt ttcttcccaa actattcatt
      721 cttctgcaag acacaatata cataatcgca taaatgatat ttttctttct ctttgaataa
      781 taataagatg taattctcat tcgacgagta taccgccaag cgttgtagtc cggatgtgat
      841 ttcattattt ttagtaattt tgtgtgttgt cctttagatt ctatatatag attcttttcc
      901 atacgaagaa ccgcttttaa atattcttta ctgctaattt ccattcatat ctcctattaa
      961 ttcaatttat acttaacaat tttattcgga tttcttgcaa caagagcatc attcaatgct
     1021 actgaatcta caacgatgga tatgttatct tcatcgttga ttccaccgga tacaatatta
     1081 cttataaaaa tcgtaacatt ttttccaaaa gaagggatgt ccgtgatgta taatgcccat
     1141 tttccgatat ttccagttat ttaaacctaa cgaattttta caatagtaca agtatcacaa
     1201 tcattaatat ataactggaa tataaatctc caaaaattca ctatagtaca gttttttatg
     1261 attaaaatct cctatttcta cccattccgc ttaaatatgc taatgtttta accccaataa
     1321 tggattggtt tagtaaaaaa tagttaaata ttcgttgttt aattggattt tgtttatatg
     1381 ggtatgagtg aatacaggca atcaactcat cgttcataag tatcttagac aaataggagt
     1441 ttaaatcaaa ttctttatta aaactagcat atctaactgc cttttgaata caagtacgac
     1501 agcgtcccaa caaaagtctc cttgttttgt ttacaaatat ctcattttct aatatatagc
     1561 ttaaatcgcc ttcaattttt ttaactaatt caatattaca atcgaactca ttatctctaa
     1621 aattatgtgt caatgaatta ttattattac atctataata atagatacaa tctttaataa
     1681 aacttgttat ttttgaatgt tgcaaatatg aaatatggaa tatgatatct tcagatatat
     1741 aatccctttc tgacggaaac ttgatattgt gctgttgaat tatctcagat gaatacagcg
     1801 catgccaaac tgacattggc aatataaaat cgactttata actcggctca gtgccaatca
     1861 ttccaagcaa cactttttca attatagcgt tatttgtaaa tatcgtacta tcatatcttg
     1921 ctggctttga aataatatta ttatcatcat aatattcata atatccagca taacatgtgt
     1981 ctgcattact cctttttata ccatttaaca ttgcttcaat ataattagct gcaacaaaat
     2041 catcagaatc aataaaagca acatattttc cttttacatg ttcaagtcct gtatttcttg
     2101 ccaatcccaa gccagcatta tttttatgaa taatacgaat ccttgaatcc tttaatgcat
     2161 agtcttcaca tattttatta gatgaatcct tagatccatc atccacaatt ataatatcta
     2221 ggttcttata tgtttgatta cacactgatt ccaaacaact tcctacatac ttttcaacat
     2281 tatatacagg tatgattaca ctaattagat cactcgaatt catccttcta tcctttcact
     2341 cacgttttct caattagtca atgacggcat caacgatttt cttaaattct ctgaaaaaaa
     2401 tagactctga tcttgatgat gtgaatgaca tactatcttc ttctagaaaa attatttcct
     2461 ttaaattatt tattaaatca ataatatgat cctcgtaagt atgctttccc ttggaataat
     2521 gaaaatatac ttttgtatta cacaatattg caattttatt tcttaatatg attatttcac
     2581 tcattcatta ctgccacata tacacaactt ataaccaccc cctgcaaagt ttagggtaag
     2641 ttatcagata atatcttcct cttcatcatc cagaatacaa aatctattta tttatagaaa
     2701 tatcattaat gtttttatat aatgtaccgt gcttgtactc ttgctctatt acaagcacta
     2761 aaacactact attcatttga attcttacac ggataaaaat ttacatcgtc tatttcaata
     2821 ttttttctct ctattttatt cacttaataa gttcattaca taatcgaaac attttttatt
     2881 ttccttgtca tcactccttg ctcccttttt gatacagatt taaaattcta tcaaagaaat
     2941 ttccatcgtt aaatttcgtt attgccaaat ccttcgaaca acgtgaaaat tttacgtatt
     3001 tatctttatc attgattttt agtattgcag atgtcaacgc tgatacgtca ccaggttgaa
     3061 acaaatatcc attttctcca tcacgtactg ctgaagaaat atctcctaca tctgtagcaa
     3121 ttacaggcat tccataagaa atcgcctcta gaatagatat aggcaatccc tcgttatagg
     3181 acggtaaaat catcacattt gatcttctat agcactcctc ctttttttct cccacaaccc
     3241 aacctgtaaa ttcaaaatac gattgcaaat tatattcttc tatcaattct ttgagcgata
     3301 tttcttcatc acctgctcca gcaatctcaa aaataaactt tttttctgga tattgttctg
     3361 caatgttttt aactgcttct accaaatcgt gtacaccttt tcgctttatc aaaactccca
     3421 taaacagaaa tcggcacatt tttgaattcc actttatggt atattctgga actacaatag
     3481 aattttctac aacaacagtt ttacaatttg gctcaatttc tttaattatc ttcttccatt
     3541 tttctccaag tacaatcgtg gaatcagact ctcttaacag caagcgaatc tgattttgtt
     3601 tttttacaga aacactatca taccattttt taaattccga tccatgaaga tggatgatta
     3661 caggaatatt taataatcta aaccacttat tcagcacata tttcctacta aaactccctc
     3721 tgtacgacat gtgcatatga ataatattag gtttactcaa taaagagata cctataattt
     3781 tagcaaatgc atacataaaa tataaagatt taaatattgc atttccaccc ttataactcg
     3841 gtataaaagt catctcgata ttcttttttt cccaattaaa attgagcatt tgtgtaatta
     3901 tacttgtaat tccacctttt accgagctgt cattaccaat cataagtact ctttgcataa
     3961 cgctttactc cttatattta tattttttct attaatacct ctatcgaatt aataatcgtt
     4021 tccgtgttag atacatactt gttaaacatc cgaactcttg tttctttaac ggcatcttct
     4081 aaattttcta attcataaca aggatagata atattcatct catcaaattg tctaattaac
     4141 tgcagttggt gatcatcgac atgctcacca tacttagcca atcgaggaac agcaatgacc
     4201 cttttacctt tttttacggc cccaattata gcacctgtac ctccatgagt aatcaccatt
     4261 tctgcttttt gcatagtttc agtaaactca tccctattta gaaatgcttt ataggaataa
     4321 tttagtggtt tataatcact ataaccactc tgtgcaaatg tactttcgat tattactcct
     4381 ttttcaatta aatcatcaat agcttttaac aatctatcaa attgaaattt ttgtgaacct
     4441 aatgttataa atatcatttt tttctcctat tatttaatat atcccaccca aataaatagc
     4501 attaggataa aattgtagca tagtttccca ctgaacataa aattgatctg caaaatgata
     4561 catcaatttt cctgtctgag tagggctggt tacttttgca aacgattcaa tataaatcag
     4621 tttttttccc atcatttttg ttattaagca gatagggatc atagctaaaa cacctgtaca
     4681 aatcacgaga tccggttttt cctttatata tataataatt gatttgaata aattaataag
     4741 tatagtacac aaaaaaaact tttcttttct atttacttgt ctcatgtagt acgttttttc
     4801 gccttttgac tcagctgaat attctgtctg ttctgttata acaaaactct catacttctc
     4861 catcagtggt tttaacatca ttaactgttc ataatgcccc ccagaggaag ccgcaaaaca
     4921 tatctttttg ttcatattaa cctctctatc agtcacgctg aaaaatatct ctagtgtaaa
     4981 ctttttcttt aatatcatcc aagcaggtat catatcgatt ggcaataata gaatcacaca
     5041 tcgccttaaa ctgctctaaa tcgttgataa ccttacttcc gaaaaaagtc tctccatctt
     5101 tcagtgcagg ctcataaatc accacagtcg cacctttagc ttttacgcgc ttcataactc
     5161 cctggattga actctgacga aaattatcac tgttgctctt catggtcagc cggtatatac
     5221 cgattaaaac tggattttcc ctgttgacat catactgatt atattcagcg tagttgtaat
     5281 agcccgccat ctgaagaaca cgatctgcga tgaaatcctt acgggtacga ttagattcaa
     5341 cgatagcctc aatcaaattt ccaggaacat ctgcatagtt tgccaacaac tgcttggtat
     5401 cttttggcaa gcaataacct ccatatccaa aggatggatt gttgtagtga gttccaatac
     5461 gaggatcgag acaaacacca ttgattatct gctgagtatt cagccccttc atttcagcat
     5521 aagtgtctaa ttcattgaaa tagctgactc gcagtgctaa ataggtattt gcaaacagct
     5581 ttactgcttc ggcttcagtg aatcccataa aaagagtatc cacgttctcc ttaattgcac
     5641 ttttctgcag aagtgttgca aaaacatgag cagcatttaa caagcgctca tcagtcatat
     5701 cagtaccaac aataatacga gatggataca gattatcata taaagccttg ctctcgcgca
     5761 gaaactctgg actaaaaata atgttcttac tccccgttgt ctcacggact gattcagtgt
     5821 agccaacagg aattgtagac ttgatgacca tgatggtatc aggattcacg tccatcacaa
     5881 cctcaataac tgactcaaca gcagaagtat cgaaatgctg agtacgactg tcatagttag
     5941 tcggtgtagc aataacaacg aaatctgcgg atttataagc gctccttgca tccaaagtag
     6001 cagttaagtc aagttctttt tctgtaagat acttttcaat atattcatcc tgaattggag
     6061 acttccgttt attaatcagt tcaatctttt cagggataat gtctaccgcg gttacctggt
     6121 gatgctgcgc caatagtgtg gcgatactca aaccaacata accggtgccg gctactgcga
     6181 tgttgtactt tttcttcaca atatctcctc tttcctctct taagtatgga gaatgttact
     6241 cctacggcta ttcatcatgt caaaataaca cagcttagaa tgttgataaa ataattttcc
     6301 agaatgtgat aaaatctctt tcatttataa accacacatc taattttatc tattctccac
     6361 aagacatgta gagtattctt taataacaag atacgacctt actaaatact tcaaaaaatt
     6421 atcatattta attcataaat ataaatcttc attttaaatg tatgcccaaa aatttatctg
     6481 taatgcgtca tcaaaatcta gattccaaat ctttatcaat ttttatccac tcattctacc
     6541 actttagtaa tattttctat ttataacact ttcgtcctac attgatcccc ccctctgaag
     6601 tacagttttt actgtcttaa ataaaatttt aaaatctaac cccatactcc aattttttat
     6661 atattccgtg tcaagttttg ctacttcttc aaaatcagta atatcacttc taccacttac
     6721 ctgccacatt ccagtaattc ctggcttaat agcaattctt gcacgatgat gataatcata
     6781 ttcttcatac tctgatttaa ggcatggtcg aaatcctata aggctcatct ttcctgttag
     6841 aacgttgaaa aattgaggga attcatctaa agacgtggat ctaataaatt gaccgattcc
     6901 tgtctttttt gttccatctg gaagtatttt atttccgata attcttggat caaactccat
     6961 cttaaacata agattattgc tcatcttatt ttctttcatt aattctactt ttctttcttc
     7021 tgcatccata tacatacttc taaacttata catcttgaac ggtttaccat tctgacctat
     7081 acgttcttgt gcaaagaaaa ttgggcctgg tgaattaata taaataagtg gagcaataaa
     7141 gataaaaata attcccgttg caatacatcc aacaatacct gcaagaatat caattgttcg
     7201 ttttataaat gcctgttgag ttgtcataac attgatactt gttgtaagta ctgtatattc
     7261 tcctatacga ccaatagatt gttttcccaa agcactatct gatacttttg caagattcaa
     7321 atgtacagta aggcccattt cagagcatcg ttcaataaat tctttcatac acgaagtact
     7381 tttattcaaa ataataaaga cttcatctac ccatccctga cataagtaat ccgctgcatt
     7441 tttggaatca gaaacaactg tcactccctc aacatcttca ccaatcatgc ttttatctat
     7501 gataatgaca ccatttattt tatacatttt atagttatgc tctttcacat tacgaataac
     7561 tacttcagca atactactag atgtaactat aataagtgat ttgttcccct catttttcat
     7621 tttatgtttc aagtaatgtt tccataagag gcgagttata tatgtaatta caacgtataa
     7681 acctcccatc aagaaaagaa cgactctaga ataatcattt ccaccctgaa cactaatgag
     7741 atacaaactt cctaacagca ccaacataat cgcttgcttt acagaagaag caaattctat
     7801 atagtaccct ctttttaata cacctctata tgcttcaaac atgaaaataa ctaaaagatc
     7861 tgccagttca atgaagactg ccatgttttg atacattgga atcacataag gattccaatc
     7921 tacctggcgt aagacatacg atagataaaa agcaagctgg atacacaata aatctaaaat
     7981 aataaaatca atatgtttaa cccatcccct tgaacttttc ttgtacattc ctttttactc
     8041 ccctcttttt aataaaataa attgtattac ttgttttctc tcctaaatat ctttctgaag
     8101 gattattgac tcgacaaatt ggcttgtgga tattcttata tatagaacat ccgtaagcca
     8161 acttaaaact ccttaacaaa atttttttac tcatattgta gatcaaaaca agtatccaag
     8221 aaatcatatt ttatttcttt tttctgctta ctcaacaaca gctaaaagaa aaatcatcat
     8281 aaacaaattc aactaagcca acgatcccct tgtgaaatca agttttttct tgatttacga
     8341 ataaagagct aaaataaacc aaacttcttc ttttttacct cttcatagac tggataactg
     8401 atttcatccc cgttgatcag atcctttgcg acttgtttca tctgtactac tttttcctta
     8461 ccaaagtcct ttgcaatctg ctcatagcac tcttttaagt aaaaggtgcg cttagagacc
     8521 ccatgggcat cagaagcagc catctgcacg agattatgtt tgaccatcgt tttggctgtt
     8581 ttctggatgt ctttgccaaa taccccaaca taacttggcg cagtgagttg ggccaaacat
     8641 cccatatcta ggaacggaag caaatgattt gggtcttcgc gaaaatgcac gttgcgttct
     8701 ggatggacga ttactggtgt ctttcctagt tctaacaact caaaaaaaag ccgttctgca
     8761 tataacggca cttccagtgt cggaaattcg atcagaagat atgtatcatc taaatctgta
     8821 aataatattt catctcggtg gatgtcttcg atgagcgttc cggtaatacg tacttcttgt
     8881 ccttccaata acgtcaaagg caactgtctt ttctctaatt cggcttgtaa cgaagcgact
     8941 agagaaatga cttgtgattt ttcattgctg tattttccat tattgtggtg tggtgtgcat
     9001 aaaatatgcg tgatgccttg ctgaatcgct ttttcagcca ttgcaatact tgcttctaag
     9061 ttttctgccc cgtcatcgat tcctggtagg atatggcaat gtagatcgat catttctctt
     9121 gtctcctctc ttcttattcc ttaatttccg tagtaataac cagcatcttt gctatgttct
     9181 gctccgttgt acacaacacc taagactcgt gcttgtacca tctccagcag ttcttttgct
     9241 ttagtcaagg attctttacg tgacatattt tcacgtacga ccaataaagt gccatccgtt
     9301 tttaaagaca tgatctgtgc atctgttacg gctactactg gtggcatatc gaatatcacc
     9361 acgtcaaata actgtcttgc ttcggatagg atctgatcca ttcttggcga gcttaacaat
     9421 tctgatgggt tgggtggctt aggtccacta ggcaaaatcg acaagttctc gactggcgtt
     9481 cgttggatca catctgctac acttcctgat gaactaagtg ctgtacttaa tccactggca
     9541 ttgttcagct ggaatgtctt atagacgacg ggtttacgta aatctgcatc gactagtaga
     9601 actcgttgac ctgatttagc aaaaacgact gcaatgtttg ctgcagtggt ggatttccct
     9661 tctccaggac ctgatgaagt cacgacaatc gttttgattt gctgaccagc tgcggaggca
     9721 aactggatat tcgtccggat cgtacgatat tgttcggcga ttggtgaaga tgggtttgtt
     9781 aatgtgatca aactgactgc atgagtttgt gctgcttttt gtttctgtgt tcgtgccatt
     9841 tttcttcctc ctagactctt gagcggcttc tgcgtgatgg ggctgtttta tcgtttgtag
     9901 cttttggtcg tgcaggagct gttccttttt tacggatacc agcgttcaat tctttcgctg
     9961 acatttctgg aactgttcct aagattggga atcctaatgt atctgtcaca tatttttcat
    10021 ctttcactgt ccgatcgagt aattccaata agaatgccag acctacacct accatcattc
    10081 ctaacattaa gccagtcatt aaattcaatt tattatttgg cgaaacaggt gtcgtattgg
    10141 caactgcacc tgaaatgatc gagattttat caacattcat cacatctttt gcgttctctt
    10201 ggaaaacttg tgaagttgtg ttagcgatct gttgagcagc cactgcattc gtacttattg
    10261 cctgaatcga gaacatttgt gaattttctg cctgattaac tgagatggca tctttgattt
    10321 gttcagcagt cattttcaca ttgtcttctg tttctaaacg atctttgact tcattgagca
    10381 ctaaatcact gacgatcata tctttatacg tattgatcat ttgcaggttc gtattgacat
    10441 cattgacatt tgcagtgtct gattgtggca gtgtcacgat caattgtgct tgtgaactgt
    10501 attttggtgt caagatgaaa aatgttacga ttcctgagat ggccaagcca gcaaacattg
    10561 agatgatgat tattaccaag tgtttgcgta atgtttcaaa aatttctttc aagctgattg
    10621 tctcttccat tttttctcca ctttcctatt tattctctag attcatttga tcttttaatt
    10681 cttttttcac acgatcaagt tcttgatcac tgactttttg ataggagata ttatcttgca
    10741 tgaagccttc tccttgcagc tgatcttgct taatcgtacc aaatgcctcc cgataatcta
    10801 aagcaatttt tttcatttca tcaaagttca tgtctgtttt catattcgac tctagagcat
    10861 tcaaaacgga ttgatagttg ctgacaccgt ttagactcaa tacttttttc gctacgcctt
    10921 ctacgatctt tctttggcgc ccttgtctac cgtaatcacc atttgggtct tcataacgca
    10981 tacgagaata agataatgcc tgatccccgt tcaatgtaat tttaccgatt ggaaagtcat
    11041 agccatcttg tgagaaagtg aggtcattat tgacttcgat accaccaact gcatcaacga
    11101 gttcctttag tcctttcata ttgatagaga catagtgatc gattgggatg tctacatatt
    11161 tttgtacggt atccatcgcc atggcagctc cgccgaatgc gtaagcgtgg ttgatcttgt
    11221 cttttgtgtc acgacctacg atgtcgacat acgtatctcg aggaatgctg acgatcgttg
    11281 tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt atctgatctt ccttgatcga
    11341 cccgacctaa atctccagta tcaatcccca tcaatagaac agagaaagat tcttgtttgt
    11401 ccagatcaac tggtttcgtt cgttctcctt gggttcgttc cactgattcg taagttttct
    11461 caatagaatg tgagagatcc agatataatt ttgctcctac tcctacaatt gctaatacta
    11521 atacgataag aactgctaaa ataatcaaaa atattttttt ccctcttgat tttcttcttc
    11581 gtcttgccat agtttcctcc gtttgctaac cttgcttgaa tcaacgtttt aactgtcaat
    11641 agcttatatt gctatagccc atttgctgag caacagtttg tgcttttgtt gcgtatgcgg
    11701 ctcttatttt ttcattccgg atctgctcta tttgactgag cgccgtcaaa tattgatcgt
    11761 aagtagcggt agctgttact tgaccgtctt tcagcatcgt atcgaatagc tgatctagtt
    11821 gcgtgacttg tgcaatttgg gtatcagcga atcctagata ttgcataacg actgttttcc
    11881 aagcactatc cttaaaaaag ctcatatttt cacgtacatg ggcgacatct gcacttgcca
    11941 acttttcttt gatgatgaca tcatccacag cttgctgcca gtttgatacg tttttagtaa
    12001 ataatttatt gacagccgtc tggatcttca gttttgattc tgcatcctct aactgcgtta
    12061 agactgcttt cttttcttct tggatcgcag cagcttcttt aggtaattct gattccttga
    12121 tgtcataatc atctgccgaa agtttgatag aagatagttt atgtgacaaa tttgaaactg
    12181 tttcttctgt tatttcagga tttaggaaaa ttttttcttt tgtactgtac agcttatcta
    12241 cttcttttga taaagaggtc acggtgtctt tttgattggt gatggcagat tctgcgtaat
    12301 tgatccgttg atgatattgc cgctgattat tgagatggta tccaaacaag caaatcacaa
    12361 tagcgcctgt gcctaccgcc acagtagcca ataacttgac gaacggcttt tccatagatt
    12421 cactcctaaa tattgatgat agataagtaa agatgtgatt cattcacatc agaatgagcg
    12481 ctttttattt taaaagcata attaaa
//
LOCUS       DAYYBG010000075        12492 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000075 DAYYBG010000000
VERSION     DAYYBG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.076"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACHAUN_002081"
     CDS             <1..375
                     /locus_tag="ACHAUN_002081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460761.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACHAUN_002082"
     CDS             complement(324..893)
                     /locus_tag="ACHAUN_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8460762.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACHAUN_002083"
     CDS             complement(967..2256)
                     /locus_tag="ACHAUN_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460763.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACHAUN_002084"
     CDS             2514..3458
                     /locus_tag="ACHAUN_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8460764.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACHAUN_002085"
     CDS             3472..4545
                     /locus_tag="ACHAUN_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8460765.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACHAUN_002086"
     CDS             4535..6706
                     /locus_tag="ACHAUN_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFM8460766.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACHAUN_002087"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACHAUN_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8460767.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACHAUN_002088"
     CDS             complement(9009..11195)
                     /locus_tag="ACHAUN_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8460768.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACHAUN_002089"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACHAUN_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8460769.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACHAUN_002090"
     CDS             complement(11417..11857)
                     /locus_tag="ACHAUN_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8460770.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACHAUN_002091"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACHAUN_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2630 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aa
//
LOCUS       DAYYBG010000076        12428 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000076 DAYYBG010000000
VERSION     DAYYBG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.077"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..250
                     /locus_tag="ACHAUN_002092"
     CDS             65..250
                     /locus_tag="ACHAUN_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8460771.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            263..511
                     /locus_tag="ACHAUN_002093"
     CDS             263..511
                     /locus_tag="ACHAUN_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460772.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            542..712
                     /locus_tag="ACHAUN_002094"
     CDS             542..712
                     /locus_tag="ACHAUN_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460773.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(814..1281)
                     /locus_tag="ACHAUN_002095"
     CDS             complement(814..1281)
                     /locus_tag="ACHAUN_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460774.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            1440..1757
                     /locus_tag="ACHAUN_002096"
     CDS             1440..1757
                     /locus_tag="ACHAUN_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460775.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            1690..2169
                     /locus_tag="ACHAUN_002097"
     CDS             1690..2169
                     /locus_tag="ACHAUN_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFM8460776.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            2186..2362
                     /locus_tag="ACHAUN_002098"
     CDS             2186..2362
                     /locus_tag="ACHAUN_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460777.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            2619..3050
                     /locus_tag="ACHAUN_002099"
     CDS             2619..3050
                     /locus_tag="ACHAUN_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460778.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            3056..3373
                     /locus_tag="ACHAUN_002100"
     CDS             3056..3373
                     /locus_tag="ACHAUN_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8460779.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            3492..4178
                     /locus_tag="ACHAUN_002101"
     CDS             3492..4178
                     /locus_tag="ACHAUN_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8460780.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            4153..5502
                     /locus_tag="ACHAUN_002102"
     CDS             4153..5502
                     /locus_tag="ACHAUN_002102"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8460781.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            5493..5966
                     /locus_tag="ACHAUN_002103"
     CDS             5493..5966
                     /locus_tag="ACHAUN_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8460782.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            5968..6468
                     /locus_tag="ACHAUN_002104"
     CDS             5968..6468
                     /locus_tag="ACHAUN_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFM8460783.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            6483..8795
                     /locus_tag="ACHAUN_002105"
     CDS             6483..8795
                     /locus_tag="ACHAUN_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM8460784.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            9042..9359
                     /locus_tag="ACHAUN_002106"
     CDS             9042..9359
                     /locus_tag="ACHAUN_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM8460785.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            9356..9517
                     /locus_tag="ACHAUN_002107"
     CDS             9356..9517
                     /locus_tag="ACHAUN_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8460786.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            9520..9819
                     /locus_tag="ACHAUN_002108"
     CDS             9520..9819
                     /locus_tag="ACHAUN_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460787.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            9819..10133
                     /locus_tag="ACHAUN_002109"
     CDS             9819..10133
                     /locus_tag="ACHAUN_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8460788.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            10126..10656
                     /locus_tag="ACHAUN_002110"
     CDS             10126..10656
                     /locus_tag="ACHAUN_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8460789.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            10653..10922
                     /locus_tag="ACHAUN_002111"
     CDS             10653..10922
                     /locus_tag="ACHAUN_002111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460790.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            10852..11055
                     /locus_tag="ACHAUN_002112"
     CDS             10852..11055
                     /locus_tag="ACHAUN_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8460791.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            11052..11339
                     /locus_tag="ACHAUN_002113"
     CDS             11052..11339
                     /locus_tag="ACHAUN_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460792.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(11372..11623)
                     /locus_tag="ACHAUN_002114"
     CDS             complement(11372..11623)
                     /locus_tag="ACHAUN_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460793.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            11826..12239
                     /locus_tag="ACHAUN_002115"
     CDS             11826..12239
                     /locus_tag="ACHAUN_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8460794.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT         4474 a         1987 c         2742 g         3225 t
ORIGIN      
        1 aggtgtcaac gatttagcta attttttagc ttacaaatta tttgtttaga aaggagctat
       61 ttttatgtct gagaatttag acttaaaaat tcgagcggag atgagaaaaa gaagaatgac
      121 tttcaaagaa ctagctgcgc ttatcggtat ttcaggagct tatttatcag atattctaaa
      181 cggcaatcgt gatggaaaga aagcacaaca gcatatcgaa acagtgaaaa aaatattgga
      241 catccgatag ggggggtagg cgatgacaaa actaaaaaaa caagattttg taaaaaaata
      301 taattattct ccatctactt atcaacgtcg aatgtcggaa ctaaaaaata cagcaatttt
      361 ctcagcggcg tatgaacggg tcacaggaca agaagtttgg atcaatacag aattatacga
      421 taaatttttg tctttcaaat cctataacag gttacgcaca agaaaggtaa cacctaaaga
      481 atttatcgag aagcatttag ttgatttata aaaaacagaa cattttgaga ggtgaaggtt
      541 aatgggtaaa ttcaacagag cattagtatt cagcgcaccg ctaatcgtct atgctttagg
      601 gctttgggga agtagacaag cgttgatagg aacgattgtg tacatggttt ggatttttat
      661 agggctggat gaagctgagt acaaaacaaa aaagccagtc gggagggact gactaatgag
      721 taaggacgat attgtatttc tgttaattat gttttgtggg attggatcaa taataattaa
      781 tttgtatttg attattaaag aaaaattttt caattaaaat gagtctttta gctttaactt
      841 aaattttttt tgataattag ctgttcttat gaccattaca agtttttcac caggtaatat
      901 atctttcata tctaaaactt ctgaaaaagc gactgagtag ccaggagaga ttgcttgtgc
      961 caacggtata ggagataaaa ggctactttt atgaatgctt cccatatcaa aaccattggc
     1021 gtgataatgt gaacaattaa ttttcttacc gttgtaaaga tacgtacatt cgataattga
     1081 gtttggcatt tttgatgggt ttgcaattac gccagtaaga atcattcgat tacttctatc
     1141 atgcttaact aaaaaatagg agagcgtaat ttttctttta tctttaaata agttatagag
     1201 ggataaaaaa aatcctgaaa tagaaatgat taaagtaata gcgacttggt aatcgctcaa
     1261 aatttttatc aagtaattca ccaccttttt actcaaatta tatcaaaaag gagagaagaa
     1321 ataatgcaag aattagtaat tttgaaaaat aaaaaaagac acgaacgccg gcaagcatga
     1381 tcgtgtcagg gaaataaact atctttctat attttaccac agaaaggaat gaaccgtaaa
     1441 tgctaattgc aacggataca ctagaccgca tctttttaaa agacgaatac aaactgcgca
     1501 atatagatgc gtcaggaatt ttagtatttg atctttatga caatggaaaa attggtatct
     1561 atcaagcaag tgatattgaa gaaacaaacc ttgctttcga acaaattgat gattctgtgg
     1621 aattggattt agatgaggca atcctagctt ttgaacaaat tgcaaaatta ttaaaggagg
     1681 cacaaaagaa tggcaactct ttaccaactc agcgagtcat atatcaaagt cctggaactg
     1741 gcagaacaat tggatgaaga aattcttcgc gatactctcg attcgattaa tgaagcgatc
     1801 gagtataagg cagaaaactt agcaaaaata gttaaagaag tagaagggaa agctgagtta
     1861 atcgatagtg aaattaaacg tctgcaggaa cgtaagacat cgcttttgaa caatgctaag
     1921 agtatcaagc actatttaca agaggaaatg gaaaagactg gtaaaacgaa gattaaaggt
     1981 gaattattca acattggaat tcaaaataat ccagtatcgg tcaatgtaat caatgagaac
     2041 ttaattccaa aaggattctt tacccctgtg cctcccaaat tggataaaaa gcaattgaag
     2101 gaggaactga agcacggaga tattcctggt gctgaactcg tccaaacaaa aggtttgagg
     2161 ataagatgat gaaggaggag aaataatgga acacgaaaac gtaaaagacg ctttgaaagc
     2221 tgctatcgaa atcgcggaag ctaaaggaat aaaggttgac ggcaaaccag caactgtcca
     2281 tgacattcaa aatttgacga aagaacatct ttattttatc gccgacttgc ttggtttgtc
     2341 ggaattgtat ttagacaaat aaccccacac ggaaggagtg gctagggtgg ttgtattaca
     2401 agtaatagtc tgcgtaacat tcatttggtc gtgcataccg gtttgtatat atatcatgaa
     2461 gaagcttcgc cctgagtctt acaaattata cgcaatttac agcataacag tagctcttgc
     2521 atttacattg tttgcaattt ctaggcttcc gttttaatcg tgggaatgaa tttttaatga
     2581 actaaattat ctataaagca aaaaaaggag aaacaacatt gaaaaacaaa ctagcaaaaa
     2641 caacagcaat catcggacta gcactaggta gcggcgttat cggctacgca gcaagcaatg
     2701 cgtttcaaga tttggacacg atcaaagcaa atttcaacac agtcctacaa tacggtcaaa
     2761 cgaaatcgca acgtgtgtca gaacttgaat cacagctatc caacaacact cgcacacagg
     2821 agcagctgaa agccgaaatt gagcaaatca aatcggacaa gcagaaggaa attgaagcca
     2881 agcaacgaga agtcgaacaa aagcaacagg agatcgctac aaagcaacag gaagccgata
     2941 gcttgcgcca acaactatcc acagtgcaaa acgacaagga gcagttagaa cagcgcgtga
     3001 gcgagttacg gcagtatacg gatcaaaagg taggggagtt ggggaaatga aagagttgac
     3061 tatttatgac ccaaatattt catgggctaa acacacaatt aaagtttctt ttatgatttg
     3121 gggttacaaa ggatatgtta cttacaaagt gggcggtaac actaagggtt tatcattaat
     3181 agctattgat tcagatgatc tatatgatgc taatttcgag gataacccag ttaatttccg
     3241 tgaccttgat gaagattggt tctctatgga acttactaac gataaaggcg acagtacact
     3301 tgtagaagat gaattcgaca gactaggtga ttatattgtc ggtgttgaaa ttattgcgca
     3361 cgaaccagag taggagagtg aatacaaaat gattaaaaga atcactatat cagttgaaga
     3421 agatagtgga tcaacaatgc agcataccga caatcgaaaa acttttttaa ccaatagaaa
     3481 ggagaatttt gatgcagatt aagaaggcga cggatatcga acaaaccaag ggaacatatt
     3541 tgatctacgg tgctccagga aaaggtaaaa cttcaacggc taaatatttt ccagggaaaa
     3601 cactgatcct tgatattgat cggacttcaa aagtccttag aggtgagaaa aatgtcgata
     3661 tcgtatatat cgacaacgaa gatacctgga acgactgggg caacacgttg gctgatttaa
     3721 ctaccaatta tgtaggtgtt tatgacaacg tggttgtcga taatgtcagc gaattggaac
     3781 ggtgtattct gtccagttta ggcgcagaag gaaaaaacaa cggcgttcct tcgcaaggcg
     3841 attatcaata tatgcaattt agaatggtga acagcctacg ctatatgaaa aatttagaca
     3901 gcaaccttat ctggaccgca tgggaagaaa tcgatttgtg gacggattcg gatggatctt
     3961 cttacaacat cgctttgccg cagatcaatc gaaagattcg aaacaatatt ttgggattat
     4021 gtgacgttgt tggtcgattg atggtaaagg aagacgatga acgcggattt attttacaag
     4081 ccacagattc aacctatgcc aaaaatcaat tggataatag atctggctgt aagcaaagtg
     4141 agctgatttt agatggaatt gtacgactac cagaatgatt tagtcgatcg tgcaagacaa
     4201 gcctatgttg atggttacaa ggcaccatgt atcgtatcac cgtgcggttc tggaaaatca
     4261 gtcatggttg cagagatcgc taaacgaacc acacgaaaag gcaacagagt gttgttcttg
     4321 gtccatcgtc gtgaattgat tgatcagatc aaagatacgt ttcaaaagat gggcgtagat
     4381 aatcagcttg taaatttcgg catggttcaa acagtcgtac gacatcttaa aaccatcaag
     4441 aaaccacaac tgatcatcac tgacgaaaac catcacgggt tagcagcgtc ctacagaaaa
     4501 atctatgagt attattcgga cgttccgcga ttaggtttta ccgcgacacc catccggtta
     4561 aacggtagtg ggttggggga tgtgaacgac ttgctaattg aaggagtatc ggctaaatgg
     4621 ttgattgaga atcatagatt agctccttat gaatattatg cacctaaatt gatcgataca
     4681 gccgaattaa agaaagcttc gactggagat tttacaaaga aatcaatgga taaggcagtg
     4741 aagaatacta tttatggcga cgtgttgaaa cattataaaa cgttagcaga aggtgaacag
     4801 gcaatagcat actgtcacag tatcgatgca agtaaacaca ctgctgaaat attcaacgat
     4861 gctggttata aagcaacaca tatcgatgca aaaactccaa aagatgaacg tgcagatatt
     4921 atcgaagcgt tcagaaatca tgaaattaaa atcctatgca atgttgattt gatcggagaa
     4981 gggtttgatg ttcctgattg ttcgactgta attatgttga gacctactca atcgttatca
     5041 ttgtacattc agcaatcgat gagggggatg cgttaccgac cagataaaac gtcaatcatc
     5101 attgaccatg tgggtaatgt gaaccgtttt ggattgcctg atatggatcg tgaatggagt
     5161 ttagatacaa aaaagaaaac aaaaactgat agtgatttat ctatcgttca atgcgcgttt
     5221 tgttttggtg cttacgaacg gccaaaaggc gataatattt gtccgtattg cggagagttg
     5281 cagccgattg aagaacgaaa gtcagagtta gaaatcgatg aatcggctga attaatgaaa
     5341 gtcggagaaa cgaaaattac tttgaatttt gagaacaata aatattacaa catgacggaa
     5401 gatgaagcag aaagtataga ggacttatat gccatagcaa gagctaaagg atttaaacct
     5461 ggttgggctt atatggctgc taagaggaag gggtggttgt aggaatgggc ggtatttttg
     5521 gtaaaaaagc agaagatgtt caaggttcaa taataaatgg cgtaacaata ataggtgata
     5581 ctggaaaaag agatagttca ggcaatcaaa ttgtaatcgc tagaaataaa aataacgaag
     5641 tttttgaatc ttctctatct aatataaaaa gaggtgttgt taacgggaca ggcgaagaga
     5701 gtggctacgt taatttaaaa aaagctaatg aaaaatacca tgttgaagga acttacgtta
     5761 aggctttgtc tgtcggtgga acaaaaggcg ttaattggga taaacgaaga aataaatgga
     5821 aagcacgatt atatttcaaa ggagaatatg tcttacaaaa ggatttcaac accaaagaag
     5881 aagcaatcca agcacgactt gcagcagaag aaaaatattt caaacctatc ttagaaaaat
     5941 atgaaaataa aaaggagaat gattaacatg acaggattta acttagactt ttcaaaagcg
     6001 caacaaggga acgaaatcaa agacggtacg tacgaagtgg tcgtaaacaa agctgtagaa
     6061 aatgcaacta aatcaggcgc agaatttatc gatatcgatt tgatcgttcg taacgatgtg
     6121 gatcaaccgt tccaaaataa acacattttc gcaaaaatct ggaaagccaa agctaccggc
     6181 aaatataacg aaggtatgat catggcaatt gcacaagcgc tacagttgga agacggaaaa
     6241 tcatataacg ggtttgatga attgctggcg gactttgttt tgaaaaccgc ctcagtacgc
     6301 gtgaaaactg aagagtcaaa tggatataaa aacgtcaatg tgaagtcttg ggataaaacg
     6361 aacacacggg gcgtaatgaa tcaccaattc aaaaatggtg atgaaccatc cttcggaccg
     6421 gaacgttcaa gagcgacaac ggttagaaat gacaatcttc cattttaatt gaggtgtaat
     6481 caatgtatga aaaaattcca aaagagctta aaaatctcaa acaatggtgt gtttataagc
     6541 tagtttggga tgaaaagcgg cagaagcaca cgaagatccc ttataacgcc aacaacgggc
     6601 ataaagccaa atccaatgac gagagtacct ggtctgattt tcagaccgct ctcgctgcta
     6661 ttgatcacta cggaatgagt ggactggggt tctttttcaa accaccttat ttcggcattg
     6721 atatcgataa tgctgaaggt gaagttgagc gatttaaatc aggtgatata gaagaaaata
     6781 ttatctatga atttatcgaa agcatgagat cctatgctga gtattctcaa tcaggtactg
     6841 gcatccacat tattgctcgc ggagaattgc ccggcggaag acgcaggaaa ggtgacgtag
     6901 aaatgtacca gaacggccga ttcttcgtta tgactggaaa tgccgcatca aaatatttag
     6961 aaataactga gccgaatcca aaggatatca aacgcctata tgatcgctac gttggagatg
     7021 aaaaaatcat ccaattcaaa gaagaaaatc cgctgatgaa taccgttgat ttaccggttg
     7081 aagaaattat caaacgtgct gaatcgtctt cccaaggggc aagattcaaa gtctttatga
     7141 acggcggttg ggaagctttt tattcctcgc aatccgaagc ggatatggcg ttcgccaacg
     7201 acttggcttt ttggaccggt agagactttg aaaaaatgga tgagattttc agagcgtctg
     7261 caatgatccg tagcaaatat gaccaaaaaa ggggatcggc aacttacgga gaatctctac
     7321 taaataaggc aattgctgat acaaattccg tctacaaccc caaacggaaa tctgatttta
     7381 agatttttat caaagaccag gaacagccaa aagaggaaaa atactattcg tacgacgaca
     7441 ccggcaacgc ggatcggttt actgatatct acggaacgtt agtcaaatat tcgtacattg
     7501 ataaatcttg gtattactac gatgggaaag tttggctaca ggacaatacc ggcgaagttc
     7561 ggaaaatgat cgatacgaca gtagacatca tgggaaaaga accgttaacg atccctgaag
     7621 gcgcagacga tgaaaccaaa gaagctttga tgaaagcgaa agaaaaacac gtcaagcgtt
     7681 ctcgcagcaa tgccggaaaa aacgctatga tggacgaatt gaaacatcga ctatccgtat
     7741 tgccggaaga gttcgacaaa gataaaacgc tgttcaacac acaaagcggc tatctgtctt
     7801 tgcatgacgg tcttctacac gagcatgaga tagacaaaat gtttacacgt gtttcaaatg
     7861 tcgagtacac agaatcggtc gattgtccta tgtgggaaga atttattaat cagatttttg
     7921 acaatgatca agaattaatc agatatatcc agaaatgtgt cggatattcc ctgaccggat
     7981 ctaccagaga gcaatgtatg ttcatcctct acggtcatgg gtcaaatgga aagtcggtat
     8041 ttttggaaat tatttctgaa ttgatgggaa actatgcgat gactatgcag gcacaaacga
     8101 tcatggtcaa gcaaagccaa tcatcagcaa acagtgatat tgcacgattg aaaggtgcgc
     8161 ggttggtcac ttcgtcagaa ccgaatgaag gcgtccgact ggacgaaggt ctggtcaaac
     8221 aattgactgg tggtgataaa gtcacggctc ggcaccttta tggaaaagaa ttcgagttcg
     8281 aacctgaatt caagttgtgg ctggcaacga accacaagcc catcatccgt gggaccgatg
     8341 acggcatctg gcgacggtta aatttaattc cattcactgt ccaaatcccg gatcacaaaa
     8401 aggataaaaa tctgaaattt aaattgcaga cggagttgca agggatcctt aaatgggcaa
     8461 tcgatggttg cctgttgtgg cagagagagg gcttggagaa accaaagtcg gttgttgcag
     8521 cgagtcagga ttatcgaaac gaaatggatc aaataggtac atttatcgaa acgtgttgcg
     8581 aaacaggtcc aggactaaaa atatcaggcg gagaattata caaagtttat cgtgaatggg
     8641 catcagataa tggcgagcat actttcacta acacgaagtt tggcagagag atttcaaaga
     8701 aatatagcaa agaaaaagct ggtggattta tggtttataa aggaatcaca ttaaaaccaa
     8761 gaaaatacga taacgttaga gaacttttta aataaatctg gagggttctg gagggttgag
     8821 aacgaaaccc tccaaccctt agaaacgttg atatatcagt atttatatta tatttttctt
     8881 tctttctgga gggttttaaa aaaataataa agtattaaag taaaaaataa aaagtaaatt
     8941 atataaaaaa gaataggttt ttgggtctga ccctccaaac tctccaccaa aatcttccta
     9001 aatgctttga taccaacgat taaacagtct ggagggttac attgaaatca gaacatcaaa
     9061 tacaagacga gatcagaatt gccttatcaa aacatggttg cacgattttt cgatccaatg
     9121 cgggacaagt gacaacgatc gaagggcggc aattttacgg gatgccaaaa gggttcccag
     9181 atctatgtgg acatcgtaat tctgatggga aagcaattta tatagaagta aaaaatgaac
     9241 gcggtaagtt gcgtgaagat caaaaaaggt ttgctgaatt tttaaacggt caacctgtac
     9301 tatacggtgt tgcacgatca gtggaagaag cattgaaaat tgtggaggaa tcatcatgac
     9361 aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg acgatctgga
     9421 acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt tgcacataga
     9481 gaatttaaga agggaaatcc atcgattgga ggaatgagcg tggataagaa agcagcaatg
     9541 aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga aatagttgca
     9601 gaagtccaaa agctcggtaa atcaatgtgg agtgaaaaaa ccaaacggag aacaccgaga
     9661 aaaattgcaa tctggcatgg tgaccgaatt ctagtaacag gtactgctga acagttatct
     9721 gaaattactg gattaagcaa aaacattatc tgggatagag ctaaacgcga gaacgttgat
     9781 tcgaaaggtc gtcaatttaa acattgggag aagaaataat ggaggaacta atcacaaaag
     9841 tagagcagtg ggcaaaagat aagggattgg atcaagctga ttccagcaag caaatgttaa
     9901 aaacgatcga agagattggg gaagttgccg cttctctagc tagaaaagat gaatatggtt
     9961 taagagacgg aattggagac gtagtagtaa ccttgattat tttagctatg caaaatgata
    10021 tggatttgta cgagtgtctg aaccaagcat acaacgaaat caaaggacgc acaggaaaaa
    10081 tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgatgaa taaaaaagta
    10141 ttaattgata aacaagcatt gattgatgaa ttaatgaaaa tacctggtgt gggatctaat
    10201 agtgacgctt tagaaacgat taaacgtttc ccatcgtatg aaccgcagaa gccagttgtg
    10261 cctaagtttg tggcggaact actcgactat tatcgtcagt caacagatgt tgatttatta
    10321 gctttattga taactttcca tgattggtat tatcgcaaaa ctaaagacgg tgagcatgaa
    10381 gaagcaattg attggctagt tgatcatcct gaaatttata tgcgtgcatg gctgtttggc
    10441 tacgagctcg agaaagagcc gttgtattgc gtaataatca atggtagata tcttgtcaaa
    10501 gtattcagca atacaaacgt tgtcatatta gttccagcag atgaattttc acagtacgtt
    10561 actcaaaaat ttcaattaac tgaaaaacaa atcaaagcaa ttgatgaaag atactggccg
    10621 tttgctgtgc cagtggatga ggtggtagaa ggatgagcga cccatacatt actaaattag
    10681 aaaagttgtc cgaagatttt gagaagaata aaaataaact aactaaagat aaacttatag
    10741 aactggttag gttggttatc agaggagcta ttacaagaga aaaggcgcag caaaacatag
    10801 aaatttttgc tcgtagtgtc cgagatggat tagagaagaa agttaaacaa aatgaacaac
    10861 agacaccgca gaataacaaa actaagaaaa cgggaactga atgtactaaa gacaaagttt
    10921 gaaaaagaat atggaatttc agcagaagaa acatataaag tggcaagtca gtgtgttgct
    10981 gatgcgagcg gcgctattcg taagtttgga atttcgatat taagtgatga tcgtaaatgg
    11041 gaggaaaaag aatgaaacta aaagacggat tttactccag cagtcatggt atcggcggtt
    11101 taatgctaga tatgccgaca aagaacccta aaacacgtga gaaaccaaaa ttcaaagtcg
    11161 gtgacatggt tcgctgtgaa gcagaaggat tcatctatcc attttgtgga tatgtagaac
    11221 atctctataa tcactcagca atcattcgta ttgaaaacac gatggaatgt gacaagtggt
    11281 tagcgaaaag caaagagaat ttagcagtag cgagattggt ggatatggag gttatataaa
    11341 cagcgaaaaa atgtacttct aagagaaaaa tttatttttt ataaaaatag gaaagtatca
    11401 ttatgaaaaa gaccacagag ataaataaac ctaccacata aaaaatgaat atttcttgcg
    11461 attcaatata ggtatattta acaatttgta caataaaaaa tactataggt aaaaaagata
    11521 acatgaggag cttatttgtt ctagagtgtt tgacaaagta acaaaatgcg acgataccaa
    11581 tacaaacggg aatccaaaat aacagcatgc tccacatagt cactcaacca tctcctatac
    11641 gctttattat caatagataa tatcattttt cgtaataaag ataaaaaaat aagtaaggct
    11701 tctcattaaa atataaaaaa gacagccgac cactgactgc ctatataaga gtattgaaat
    11761 aaaaagctgc tgatataata aattccacaa gtttattata tcacataaag gagcggtttg
    11821 acttgatgca attgttacga gaggtagatt tcaaacagac aagatgtaat gcgagagatg
    11881 tgctgaagaa ctttcggcgt ttggagcgga tggcaggtcg ctctttgata gatattaagt
    11941 cgccgattat tacggatatg ccgaaggcgc cgaagcacgg caataaggca gaagacgcaa
    12001 tcattcagat gatggatata gaagcggaga gagacgcgat tctagcagcc ttgatggctc
    12061 ttagtctgat tagtcgtcag atactctact atagcttttg tgtgccagat agcttctcaa
    12121 actacagaat tagccgtgaa gtgggttatt cagaaagaag tatacaacgg atgaagtcgg
    12181 aagctctaat agagtttgca gaagcatata aacacggaag aataattgct tataaataat
    12241 ttggcggctt tttggcggaa tgatggcggt ttttagctat ttaccagtga tattatggta
    12301 gtgtcgaaag attagtgata ggtctaagac aaaataataa taaaaggaac atcgttttat
    12361 tattgtttca caattaagct tcgatagaca gcaacggaaa tattaagaat aaggatgtga
    12421 attttaac
//
LOCUS       DAYYBG010000077        12408 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000077 DAYYBG010000000
VERSION     DAYYBG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.078"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(374..988)
                     /locus_tag="ACHAUN_002116"
     CDS             complement(374..988)
                     /locus_tag="ACHAUN_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8460795.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1594..1971)
                     /locus_tag="ACHAUN_002117"
     CDS             complement(1594..1971)
                     /locus_tag="ACHAUN_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460796.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2271..3152)
                     /gene="galU"
                     /locus_tag="ACHAUN_002118"
     CDS             complement(2271..3152)
                     /gene="galU"
                     /locus_tag="ACHAUN_002118"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8460797.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3195..4214)
                     /locus_tag="ACHAUN_002119"
     CDS             complement(3195..4214)
                     /locus_tag="ACHAUN_002119"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8460798.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4847..5851)
                     /locus_tag="ACHAUN_002120"
     CDS             complement(4847..5851)
                     /locus_tag="ACHAUN_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8460799.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5864..6913)
                     /locus_tag="ACHAUN_002121"
     CDS             complement(5864..6913)
                     /locus_tag="ACHAUN_002121"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8460800.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6930..7751)
                     /locus_tag="ACHAUN_002122"
     CDS             complement(6930..7751)
                     /locus_tag="ACHAUN_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8460801.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7738..8517)
                     /locus_tag="ACHAUN_002123"
     CDS             complement(7738..8517)
                     /locus_tag="ACHAUN_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8460802.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8534..9004)
                     /locus_tag="ACHAUN_002124"
     CDS             complement(8534..9004)
                     /locus_tag="ACHAUN_002124"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8460803.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9016..9435)
                     /locus_tag="ACHAUN_002125"
     CDS             complement(9016..9435)
                     /locus_tag="ACHAUN_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8460804.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9516..12293)
                     /locus_tag="ACHAUN_002126"
     CDS             complement(9516..12293)
                     /locus_tag="ACHAUN_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8460805.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT         3625 a         2611 c         2031 g         4141 t
ORIGIN      
        1 ttcattgcaa taatgataac aaatgtacta taattacttt gtaaagagtt ttggggaaga
       61 ctctttcatc aacgtgtatt aagagacaag aaagaatcca tatggattct ttcttgtctc
      121 tttttccact actttttatt tttctgacac tacatattct tataactcat ttctcataac
      181 tgtgaaacaa cttataaaaa taacaaactt taaacaaaac aagcttaaaa tcaaagatga
      241 tccatcttaa aataactaaa acaaaaaaag tagtcgttgt taatcctcat taataatcgt
      301 tttctttttt tcaaacgctc catttttatg tggaacatta tttttttaag gaactttgtc
      361 aacatgtgga taactatttt ccacttgtgt cctggactag ctgttttgct gtaaaataca
      421 aaacttgctg cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc gctggatcta
      481 gatttggcaa agtcaagact gcatcatgta aattggcgtg gttgtccaaa accaaatcag
      541 caaattcaaa taatcgcaaa ccactgctat gttgcgattt gatacttcgt gacaaatcaa
      601 atccagtaac aatgattaat ggatgaccat tttctttgat ccactgagct agttcgatga
      661 ttgccgggtt tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa
      721 gctgatcaat aattgcctgt cctgcacctt ctagcgtttc atagacacct ccggctggat
      781 tttcaatcaa tatacttggt acaagatccg gggtttgatc ccaaaattca aatgctacac
      841 cacttagtgt gcggctagca tatatctgca atctaccacc ttgattaatt tgtttcgcga
      901 tccaagtgcc agcttgaagc atcagcggct cttctatcct taatttctct tccaattggt
      961 gataatagtc aaaataacat aaactcattt atctctcccg cctagctata cttgacatga
     1021 atagaaacag ctgcctaatc taaggaagta cataaaaaaa cagtagttag acagtaacac
     1081 atgtggtctt tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc caataccttg
     1141 acatagcaaa taaaacaacg tgcagaccat ggaaaatatc tacatagggg tagaattttc
     1201 accctagatt aggtacaata aattaggtcg atgcactgca ttgactatgg caaaatgatc
     1261 ggtcagggtg tatgtgtgtg actggtcatt ccctgattct tagcgccaac taagggtcag
     1321 ggagcctttt tttattcagc tgtttctatc tgaatttatt gtaagtaatc tcttatccaa
     1381 gtgcaagcaa gaaaatacaa aaaaacattg gtatgattta tatatatata aataacataa
     1441 aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata
     1501 agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt
     1561 attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact
     1621 ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg
     1681 agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc
     1741 aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac
     1801 ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat
     1861 gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag
     1921 ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac
     1981 acaccttttt ttataattaa aaaattcgcc tttttattct actatgaatc ttctggcttg
     2041 gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat
     2101 tagcataata ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga
     2161 ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc
     2221 cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct
     2281 gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa
     2341 tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat
     2401 ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca
     2461 tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt
     2521 ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca
     2581 ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca
     2641 ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct
     2701 tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa
     2761 tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg
     2821 gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt
     2881 tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg
     2941 atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg
     3001 tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg
     3061 attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca
     3121 gcggcaggaa tgactgcttt tttgaccttc acttgctgtt cctcctaaac gacgattaac
     3181 tattgatcat ctatttaacg aagagaaaat tcattctcca tttttccgtc acgtaacatg
     3241 atttcttttg ctgctttttt gatatcttct ccattataca aaacattata gattgtttga
     3301 gtaattggca tctcaacacc tagctgttga gctagctcta ctgctgcttt tgtcgttgcc
     3361 acaccctcaa cgaccattcc catgttttct agaacttctt ccagcttatg tccctgtcct
     3421 aacaagttac cggcacgcca attgcgagaa tgaacacttg tacaagtaac gatcaagtcc
     3481 cccacaccac tcaaaccgat aaatgtcaga ggattggcac ccatcgcgac accaagacgg
     3541 ctgatttctg ccaatcctcg agtcatgatt gctgcttttg catcatctcc gaatcctaat
     3601 ccatggatag cgccggcacc aattgcaatg atatttttca gtgctgcacc agtttctacg
     3661 ccaatcacat cattatttgt ataaatcctg aaataatcat tcatgaacag tttttgtaca
     3721 taggctgctg ctttttgatc aatacttgca gctgtaattg ttgtgatatc atgtactgcc
     3781 acttcctcag catggctagg accagataat acaacaattg cctgtctttt ttcttctgga
     3841 atttcttcag ccaaaacttc tgagattcgt ttatgcgtat cttgttctag tcctttactt
     3901 gcatgtataa tgattggctt agtattcatt ttaccaataa gttcctgtgc gactgaacga
     3961 atcgcttttg ttggaacaac aaataaaata gcatctgcgt tatccactgc ttctgccaat
     4021 tctttatatc cgataatcga ttctgggatc gtcaaatcag gtaaaaaatg ctgatttgta
     4081 tgatacttat tgatttcatc aatctgttca ggtacatttc cccaaatacg gacatcatgc
     4141 ccgttttctg ccaatgtttg tgctaaagca gtgccccaag atccaggacc aagaactgcg
     4201 attttttgtt tcatggatat tcctccaaat aaataattga taatttccat tatagtatac
     4261 atttcttaaa gttgggacta atttccattg tttttaagtg tttgtttact tcttttatcg
     4321 tttttacttg aaataatcaa tgatatgtta caatagtcta cattttattt ttacactcgt
     4381 tatgctttgc ttgttcctaa gaggaagcag ttttgacaat gttactacca gcattactat
     4441 taggaggtta ggaaacttct cctagtgagt attgatttaa aatacataag agacagattg
     4501 aacatttcta aatctgtcag gtcctgcaac ggacagattg tctctgttga cggttgacat
     4561 aggaactttt gttttagtca acattctgga tacaaaacgc tatgtaaact ggcgcaactc
     4621 gttgtacttg gacttttact acggatccat gcaacaacta aatagtacta ttgcataaca
     4681 aaaaatggtt ggcacccaca tgtcccatta aacagattat aagttgaact gtagataatg
     4741 caaaaaatgt gttttataac gtaattgatg ccaatatttt aaaaaaataa agggagcagt
     4801 tatcgctgct ctctttattt tttatattct ttttatccag tctatcctaa taatcaaact
     4861 gacgatagta gcggcgtatc tctaatggat ggcagttcaa ctgctccaca tgagcatcga
     4921 tgcgttgtgt cactacatgc atcaataaga aagacaaaac acgtccgcga tatttttcac
     4981 tgataccttc tactggatag tcttttgtgt ctatcaatgt ataattgctg caaatttttg
     5041 gcaaaagttt agccacacgt tcagataaca ctctctgttc atcttcgcct aaaaataatg
     5101 tgactggggt tgtttcttct acgatctcta acgtcccgtg aagaaactcg gcagcatgga
     5161 tagatttcga tggaagccag ctttgttctt cccagtaaca catggcatat gaataaacag
     5221 ctccccattg attccctgct ccaataaaat aatgcatact atcacgtcga tgtttttctg
     5281 caaatgataa gccaaatgca tctgcttttt tctctgcttc cactaaccct gttggtaaat
     5341 aacgttcgag ctgttcatag tattctgaat aagcttcgaa ttctccattc aaatacatca
     5401 atcgatctgc tgccataaaa aatttgattt gttctgttcc aggagctgga taagtgacca
     5461 caaaatcgca tagctgagac aacttactgt tcgctttatc gataaatcca atcagtgtcg
     5521 ctcctacttt cttgatttgt tctactgctt tcactacttc ttgagttgta ccggtaacag
     5581 aggacaaaat aacgatagaa tcttttgtta atcttttgtt tccagtagtg taatattctg
     5641 cagcatgctg tacaaagaca ggcaagttgc ttttcccatt catataagtg acagcttgca
     5701 tcgaggaagc atacgttcca ccgattccta gataatagac agcgtcaaat ttttccgcat
     5761 aaagtttatc gatgatttct tctacttgcg gacgtaaagc tagtgcgcct tttattgctt
     5821 tgatttgttc ttcttcattg aatttcagca tgatttccac ctcttataat ttacttttca
     5881 agactgtatc aaagtcttca aaaatctttt tgttcaagtc aataccttta gctactgcgg
     5941 ttttataagc gaatagttga aacggaacga caagcgctaa tacagacaga tattcatcac
     6001 attctattgc taatccaagt gttttctcgt ttcttgcttt ttttgtggta atggtgagag
     6061 tctctccaac gtaccgactc atatatcttt gcaacttttg cgaacgttcc ttatttacac
     6121 tttccccttc aataaagaat agcaaatgcg aagcatctgc ctccaggtat gggccgtgca
     6181 tataagcttc taactcaaat ccttgtgacg gaacgcggat tgtttcagtc aatttcgtct
     6241 ctgcttcttt agctgttccc cagtttggac catatccaat ggtgacgaaa cgctgtgcta
     6301 aacgaaataa actttgatat ccatcaaaaa attcttccgt tttttgaata atcgctggta
     6361 aatggttgat tattttgcgc aactgttgca tgtagtcttg ttctattttt ttgctgattt
     6421 tatttttact tattccaatt cctatcccta agagtagtaa ctgtaaaaca gtcgcagaat
     6481 agccttttgt aacgaagcct actgattcta ccccggcacc taaatcaata atttgtgcag
     6541 cttctaaagc aagtggactt tgcacgtcat ttgtgatagc aacaacaggt aacccttgtt
     6601 tttgtatcat ttttactgct tcaacagtag aagcactttt cccgctttgt gacacagcaa
     6661 tcacagtatc gattgaagga gaaagttttc cataattgaa aaaataaaaa ggttcttgaa
     6721 tatcgacagt aagatcacca taagattcca atgctggctt cgctgctaaa caagcgttat
     6781 aagatgaacc cgttgctaag atcagtaaat tcactgtttt tttcatatgt tctcttgtcg
     6841 aaaaatcatt ctttttcaag atcgtactta gtacttcctt ttcttcatag atataatctt
     6901 gcatcgtaaa cattttctct gctccttttt taaaaaatcc ctatccacga accaaaaatt
     6961 ccaaagaaag ctagtccgat caagatcgtt aatggtttca cttctttctt caatagataa
     7021 aacactatgc caaatgctcc aagacataag atatttggaa cgatatcatc aaaaatactt
     7081 tgaacagtca cagcattttc tcctgaaccg atttttaaag gaatattgat ggtgatcatc
     7141 gtggcagtca ttgctccaac gaccatcagt ccaataatag acgcgcctaa tgtcaaactt
     7201 tccatcatac cgtttgcctg gatctttttc aaaaaacccg ttcctaagcg atatccccaa
     7261 cctgttgcta gatagcgaaa cagaatgtgc ggaatattga aaatcaaaat gaacaagata
     7321 gggcctaaaa tatttccttg catggctaaa gaggtaccca cacctgtggc aatcaatcgc
     7381 aaagttcccc aaaagaaaga atctcctaac cctgcaagag gtcccatcaa agatgttttg
     7441 atactatcga tcgcagtcac atcaaattca ggatcatcct tattttcttc ctccattgca
     7501 gccgtgattc ctaaaatcag tgtaacgata tgtggggtcg tgttaaaaaa ctctagatgc
     7561 cgtttcaaag ctgctgccat ttcttctttc gactgatata actttttcaa aatcggaatc
     7621 attgcataaa cataagccat attcatctga cgttcataat tccaagaaaa ctccatttga
     7681 aaagaacgcc aaaatacgtg atttagctct tttttcgtta ttttgctgtt tttgatctca
     7741 gaattcttca tcttcttcta cctcccctgc agttgtttga agtggtagtt gacggacatt
     7801 cataatattg actaccacaa ctgctaaaat cgcaccaaaa atagctactc cggtcacagg
     7861 aatctctaag taagaagcta tagcaaaacc taagaaaaaa tatggtgcaa cttgtttgtt
     7921 gatcagcaaa cgagcaagca tcgcaaaacc aagagctgga atcaaccctg tagctactga
     7981 caaccccttt tgaacgaagt caggaatcat atttaataga tttccgatcg tattgcttcc
     8041 caccatataa gaaaccatta ccacaacacc aagcatcaag gaaagaccga taccggcaag
     8101 caagtgcata tgctcgactc ctttataatt tccttcttct gcataattgt ctgctttatg
     8161 gttcataatt ggaatcaaaa gccctaagta aatatttttc aatatcaatg tcaacgtagc
     8221 aatcggcaaa cctagtaaaa gcgcggtttc tgtaccggct ccagctgtaa tagcaaaagc
     8281 cgtccctaaa atcccaccgg tcacaacatc aggtggaatc gaagcaccta ctgagaacga
     8341 tccgataaat gctaactcta aagttgcgcc cataatgatc ccagttttta catcacctaa
     8401 cacgattcct gtaaacagcc ccgtcacaat cggacgagaa agcaacgatg tccctagtgc
     8461 atattctgat tgtgcgacaa atgcaacaat tcctaataag actgcttgta caatcattga
     8521 ggttcttcct ttcttacaaa acttctctta catccacttt tttatctgct ggtacttgac
     8581 ggatttctac ttctatttgt ttgtcaatta atcgtttcaa gcgttcctcc tcttctggta
     8641 aaatatttac cgcttttccg atgctgcgag taccaggttt tgccttgatc cctcctaagt
     8701 tgatggtttt gatttctgga tacgcttctg ccagcttata tgcatcctcg atcgattcca
     8761 ctacgatgaa taacttatat ttatctgtca ccccgctttt caacgcggca atcgagtcat
     8821 caattgattt gatcactagt ttgactcctt gtggcttggc taatttgatc gttgtctttc
     8881 gtatctcatt cgtaggtaca tcatcattag caatcaaaat acagtctgcg cctattgttt
     8941 gtgtccatga aaaagctact tgtccgtgta ataaccgatg gtcgactctt gtcaatacaa
     9001 tcatttttct tcctcctaaa attcgtcttc ttctattttc tgattgagcc gctcgttaca
     9061 aaactggatc attttttttg aatcagttag cacctgctgg atcaaaacag ttgtctctac
     9121 ttcagcgtcc aaccgagata ctagctcaat caatagtggc aaattcaatc cggcaacgag
     9181 atagacatga tcccgattga tatattgtaa gaattcgttg ttgacacttc cgccaaaaag
     9241 atccgtcaga acaatgattt ctttatcttt gtacctcata aaaagctgcc ttactgtttc
     9301 cgctaaatca aaattctcat cagtatagca atcgaataca gtaatagaat gatttttccc
     9361 atagatcagc tctaatgaat ctaatatccc tgaagcaaat tttccatggg aagctaagat
     9421 aatttctcta tccacttttt catctcctct tttcacttac acatactaaa aaagcaagtt
     9481 gcatgccaaa attagtggca acaacttgct tatgttcaaa attcttcttc tatcgagaga
     9541 aactctgtat cttcactcaa aatatcatag atataggcaa tctcagaatt ggggatcatg
     9601 acactataat gctcttcaat gacactaaac gcagatttaa tagcagataa cccttctttt
     9661 tgacactgct tccatttctc taatccttga taggtttcga ttggcatttg tcggatcaat
     9721 cgttcaatga gacagctcac atggacatac aacgctaatt ttttgggatt gctgatgctt
     9781 cgattcaact gcacctctaa ttctctcata aaatgatcga tttctcccat caccttctct
     9841 gtatctagaa tagtcacgga atccagtact ttttccaatg aaaaattacg aatgatgcgc
     9901 tgattgaata tttccttctc tgaagcattc atagtagtgt ccaaccattc agctagtaca
     9961 gacatctttt cccccgagat gaggtcttct aatgaaagat agtcgatatt ggtgatatca
    10021 ggatctgtgg tgccaatcat tcctacgact tcgtatatgg aaaataatgg gtcactgttt
    10081 ttcttatctt ttaattgctg atattcatat gggatgattt tcaactgaca agccgtaggt
    10141 aagctttttt ctagcagttc acttaagtgg accgccgtac cgattccagt gaagcaagta
    10201 gtaatgagtg ctttgggttt gttttcctct ggatagagca gtcgccattc tggctggatc
    10261 tgtcgaacag tctcttctgc tatctcttct aatgatacat ttttttgcag gcattcccct
    10321 actgtgattg ctaaaggtgt cgtcacatta ttcaaaatca aaagcggtac ggataactgt
    10381 tttttaaaaa actgatggat ttctttcaga gatcccatgt ctactaatat gatcaatcca
    10441 tttgaaacat cgctgttttc gcaataatca aggatttctg aagcaatctg ctgtggtgtg
    10501 acatctagtg gcatatcaaa tgattcaaat agatcctctt gtaataaacg atttactaca
    10561 tttgccatac tgctagcagt tgcataaccg tgagcagcaa tcatcgcttt tggcagtcgt
    10621 ttttcttttg accatttcgc tttttttaga tagatcgtca gtaaaatccg atccatactt
    10681 gtgtcttcaa tatctaattt gatcttactt aattccagaa ttcgttggac atagtgataa
    10741 ctagatggat aagactgaga aacttgttgt tctaattctt tgattagttg gataatatca
    10801 tgttcttccg gtaaccattt gatagtgctt cgctgataga gatagtaact caaggcatag
    10861 acactgtttc catcgaattt gatttgatag gcagtttcca tttgtcgaaa ggtctctcgg
    10921 atagattgtg tccaataaac cagcagctcg tgtttttctt gtctactggt ctcaaacagc
    10981 aaaaaatcaa ataattgaat cacttcttgc tttaattttc cttcgcaaga atatagcaga
    11041 cagttatttt tcttgaaatc aattaggatt tgctcaaaac ttttaacgat tcgctgctgt
    11101 tcgggctgtt gcttctccat taattgctct aaggtctggt cttgcgtaat atagattcct
    11161 tgctgtgctg tatttttagt aataggctgc gcaagtagtt ctttttgcaa atgatggatc
    11221 gtgacatgga tttcaggctg ttcacgctgt tcagcatatg cttttgctgc tgaaactttg
    11281 acgatatttt tcagctctcc tatgtttccg gataatttct gacttgttaa aagtgacaaa
    11341 gctggtccac tgactgacaa agggctcccc atttttcttt gttcttcgag aaaaaaagag
    11401 taaatcagtt ctagccgttc attacgacta cgctgattaa gtgcaggtag ttcgattcgg
    11461 ataggtattc ttcgaatgaa agttgttaag aaatgactag taatctcttc agtcgtagca
    11521 aaaaagagac gtgttttgat ccggcgtgac aaattaggtt ctcccatacg ataaatcaca
    11581 ccttgatcaa gataggtgaa cagtttttcc tgaccttctg gtgttaagcg atgtacctcg
    11641 tctaaaaaca agatcccacc atctgctgct tcaaaagccc cttttttatt ttcttctgca
    11701 ccagtgtacg ctcccttaac atgtccaaac aaattgctag tcaaaagttc aggattatct
    11761 gcatattgtg cacaattaaa agtgatgaat ggtgcagaat cttctagcag atcatgaagc
    11821 agacaatact gatagaccaa attaactaaa taacttttac ctgtgccact ttcgcctgta
    11881 atcaaaagtg gtaatccgcc atctggatag taaagagctg ttttgatttg ttcgatactt
    11941 tctttcaaac tggcgtcgta tccgatcata aaagagaata agtcttttgg ttgcgcgaaa
    12001 aaaggctgtt cctctttgag atcatcgata gaaaaataaa aattcgtgcg taacttaaaa
    12061 aattgttttt caaaagcttc tttgtggaaa taaatcactg gtcgagaatt gattttgact
    12121 agtagttgct catcatttaa ctggttcaaa taatgactga ctgtgtttct ttttacccca
    12181 aaatgatcag ctaacctttg tgctgtaaag acctcgttca tatgttctaa atcgaaaaag
    12241 gctgtctggt tctctaagta acataaaatt tccttcttca acaacttatt cacccctcct
    12301 ccatcataga aaacggattc attttgtcaa taaaatcttt aataaagaat tgaaaaatat
    12361 atcttattat agacagcagc ctacttatcc cttataattt ttattcta
//
LOCUS       DAYYBG010000078        12335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000078 DAYYBG010000000
VERSION     DAYYBG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.079"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACHAUN_002127"
     CDS             <1..154
                     /locus_tag="ACHAUN_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8460806.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACHAUN_002128"
     CDS             complement(208..486)
                     /locus_tag="ACHAUN_002128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460807.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACHAUN_002129"
     CDS             complement(601..1218)
                     /locus_tag="ACHAUN_002129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460808.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACHAUN_002130"
     CDS             complement(1613..2437)
                     /locus_tag="ACHAUN_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8460809.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACHAUN_002131"
     CDS             2780..3196
                     /locus_tag="ACHAUN_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8460810.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACHAUN_002132"
     CDS             3193..3663
                     /locus_tag="ACHAUN_002132"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8460811.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACHAUN_002133"
     CDS             3677..4450
                     /locus_tag="ACHAUN_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8460812.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACHAUN_002134"
     CDS             4464..5276
                     /locus_tag="ACHAUN_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8460813.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACHAUN_002135"
     CDS             5330..6295
                     /locus_tag="ACHAUN_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8460814.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACHAUN_002136"
     CDS             6431..9154
                     /locus_tag="ACHAUN_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8460815.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACHAUN_002137"
     CDS             9167..9799
                     /locus_tag="ACHAUN_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8460816.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACHAUN_002138"
     CDS             10007..10231
                     /locus_tag="ACHAUN_002138"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFM8460817.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACHAUN_002139"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACHAUN_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8460818.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACHAUN_002140"
     CDS             10556..10825
                     /locus_tag="ACHAUN_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8460819.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="ACHAUN_002141"
     CDS             complement(11004..12305)
                     /locus_tag="ACHAUN_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8460820.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4029 a         1998 c         2422 g         3886 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtg
//
LOCUS       DAYYBG010000079        12209 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000079 DAYYBG010000000
VERSION     DAYYBG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.080"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..649)
                     /gene="frr"
                     /locus_tag="ACHAUN_002142"
     CDS             complement(92..649)
                     /gene="frr"
                     /locus_tag="ACHAUN_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8460821.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(652..1374)
                     /gene="pyrH"
                     /locus_tag="ACHAUN_002143"
     CDS             complement(652..1374)
                     /gene="pyrH"
                     /locus_tag="ACHAUN_002143"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8460822.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1510..2391)
                     /gene="tsf"
                     /locus_tag="ACHAUN_002144"
     CDS             complement(1510..2391)
                     /gene="tsf"
                     /locus_tag="ACHAUN_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8460823.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="ACHAUN_002145"
     CDS             complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="ACHAUN_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8460824.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3631..4110
                     /locus_tag="ACHAUN_002146"
     CDS             3631..4110
                     /locus_tag="ACHAUN_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8460825.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4327..5418
                     /gene="phnW"
                     /locus_tag="ACHAUN_002147"
     CDS             4327..5418
                     /gene="phnW"
                     /locus_tag="ACHAUN_002147"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM8460826.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5411..5539
                     /locus_tag="ACHAUN_002148"
     CDS             5411..5539
                     /locus_tag="ACHAUN_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8460827.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5543..6088
                     /locus_tag="ACHAUN_002149"
     CDS             5543..6088
                     /locus_tag="ACHAUN_002149"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8460828.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6542..8233)
                     /gene="argS"
                     /locus_tag="ACHAUN_002150"
     CDS             complement(6542..8233)
                     /gene="argS"
                     /locus_tag="ACHAUN_002150"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8460829.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="ACHAUN_002151"
     CDS             complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="ACHAUN_002151"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8460830.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9715..10734)
                     /gene="argF"
                     /locus_tag="ACHAUN_002152"
     CDS             complement(9715..10734)
                     /gene="argF"
                     /locus_tag="ACHAUN_002152"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8460831.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="ACHAUN_002153"
     CDS             complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="ACHAUN_002153"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8460832.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3344 a         2529 c         2203 g         4133 t
ORIGIN      
        1 taattttttc tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg
       61 aatgatccgc ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat
      121 cagcagcgat cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt
      181 ctaatccgcg caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat
      241 cgatcgcatc acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca
      301 catctttagc taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga
      361 catttccatc attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat
      421 cttgaagaat gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga
      481 ttgatgccat ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca
      541 aaaggctagc gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg
      601 ctgctttttt cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc
      661 ccttacagtt gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt
      721 taaattgaag acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc
      781 catgacttgt aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc
      841 atttgcatct actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag
      901 gataacatct gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta
      961 cgggttgccg gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc
     1021 tttacggcga atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt
     1081 cggtacaccc acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa
     1141 catacccatg taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg
     1201 ccagatattt ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt
     1261 gatttcttgg atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc
     1321 taaagcttct ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat
     1381 tcctccaata ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact
     1441 ggtttttctt gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat
     1501 tcaacagaat tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt
     1561 ttcgatacct tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc
     1621 aacgtatttt tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat
     1681 ttctgctttg aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt
     1741 gccttcgttc aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg
     1801 aatttgtgat tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct
     1861 tgcaactgtt tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat
     1921 atgcaagtat ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa
     1981 gctgattttt tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt
     2041 atctgttttg attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc
     2101 tgcaatttct tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga
     2161 gtttacttca acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc
     2221 tgcaatgcgg tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac
     2281 tgctttttcc atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac
     2341 gccagtcatt tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc
     2401 ctcctgtgtg tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc
     2461 agcctttgaa acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca
     2521 acatctacga tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact
     2581 acttgatctt caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat
     2641 ttaacggcac gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg
     2701 cagtttgtat caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca
     2761 atacgttctt tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg
     2821 ataccgccta agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt
     2881 ttaggtagaa cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg
     2941 atacgttttt ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg
     3001 aagtattgtc ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta
     3061 cctacgaata atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca
     3121 tctactaatt taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag
     3181 atatatttct tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct
     3241 tctagtaatt gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt
     3301 ttcctcttct tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa
     3361 taagaatgtg gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc
     3421 gtatcgcaag agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa
     3481 aagaattcaa gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa
     3541 taattttagg tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac
     3601 atgtgaaatc tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt
     3661 aaacaagtca ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg
     3721 cattatggcg ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc
     3781 gtgaaggcac ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact
     3841 cctgagacta ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg
     3901 aaagtcgaac gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta
     3961 tttgcatctg ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc
     4021 gatacgatca tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt
     4081 caccatccaa ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg
     4141 tgttttttta ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata
     4201 catcattttt tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata
     4261 ttggatgtta acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga
     4321 aatcatatgt ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat
     4381 gctatgctga tcgatcattg tacttgggat caagaataca aagaaattac acaatggata
     4441 agaaaaaaat tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg
     4501 caaggtagca gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat
     4561 actgtactga tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt
     4621 ctgaaactga actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg
     4681 attacacagc tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag
     4741 actacttccg gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat
     4801 cttttgttta tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa
     4861 ctagggattg attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt
     4921 tcttttgtac ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg
     4981 tcacttgatc tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc
     5041 acttccccta cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa
     5101 ggaggaatcg aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa
     5161 atgacagaga tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct
     5221 tttctttacc ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa
     5281 aatggatttg tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat
     5341 attggcgaga tctatccgga agatattttg gaactgacag caattatcaa agactataag
     5401 gagaacgtta atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg
     5461 ctgtatggct cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa cccaactgac
     5521 tgacaaagaa atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct
     5581 ttccctccct tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa
     5641 agaggtggag gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca
     5701 ttctaccttg aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa
     5761 tattggttcc acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa
     5821 aaaagaaagg tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg
     5881 accgtttcct tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga
     5941 agtaatcaaa gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt
     6001 gacagtaggt gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa
     6061 attagagatg gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc
     6121 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6181 aagaatccag aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa
     6241 gtaataaaaa cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6301 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga
     6361 cttttgtcac agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta
     6421 aacggtgttc attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc
     6481 tattttctct tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt
     6541 cttacatctt atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag
     6601 tcactgcata aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg
     6661 tatgagcata gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg
     6721 acggttcgta tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa
     6781 ccacttccca gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc
     6841 ctgctttttc caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt
     6901 ctccttcaaa tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt
     6961 tcaaatcgtg gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt
     7021 ccaaatcagg gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata
     7081 cttcttctaa taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga
     7141 tcaaaccaaa tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga
     7201 cagctttcaa ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt
     7261 tgaagtcgta agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg
     7321 tcgcaccgtc tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga
     7381 tctctgcacc tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat
     7441 ccatcttgtc attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat
     7501 catagatttt attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag
     7561 cctctttatc gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg
     7621 gttctgtttc agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt
     7681 ctgctttaac agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact
     7741 gtgttcccca gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa
     7801 tgaaaccgat cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg
     7861 caatattagg agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc
     7921 cataatgttc tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat
     7981 tcatgaaaaa gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga
     8041 ttttttctgc taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg
     8101 aaaaggctgg aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct
     8161 cgacttgttc cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat
     8221 ctttattatt catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc
     8281 tctagcgacg gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc
     8341 caattgtcct aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa
     8401 cagaattctc caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat
     8461 tcgctagcca agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact
     8521 ttcttgaatt atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaaaaatcca
     8581 gatggcctaa aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa
     8641 agacttaaca aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc
     8701 gatattttct aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat
     8761 agctgcttct actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg
     8821 tttcatttct gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac
     8881 atacacattg tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa
     8941 tttttctgaa gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt
     9001 ttctacaaca ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag
     9061 cgtttcgatg acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct
     9121 ccatccacgt ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt
     9181 ggcttcttct tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc
     9241 attttcatcc acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc
     9301 cttcagtact tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc
     9361 aacacatgta tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat
     9421 caataggtta ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc
     9481 gataaatttc actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact
     9541 tgcatccgta gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat
     9601 ttcattcgtc ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt
     9661 tgtgattttc cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac
     9721 gcgagggata aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg
     9781 attttctgct tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc
     9841 catttcagtg atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg
     9901 gaaagctggc aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat
     9961 ttccatattg atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc
    10021 acccattgat acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac
    10081 atcgtcggta atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac
    10141 gatttcttct tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat
    10201 tgcacctgtg actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac
    10261 agtgatacct tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg
    10321 tgttggatgc cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag
    10381 ttcctctacc attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag
    10441 tactttagct gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag
    10501 atattcagga tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt
    10561 agatgttttt tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc
    10621 acgttttttc aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt
    10681 tgtaaagtct ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa
    10741 cattctcctt tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca
    10801 gtaagactgc cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat
    10861 gcagcgtgga ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat
    10921 gccatagcta cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc
    10981 tggagcgatt gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc
    11041 atctccatta ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt
    11101 ttcgtgtaag ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa
    11161 gacatccatc ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc
    11221 taccattgta aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc
    11281 caacactttt tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc
    11341 tttcgcatta gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc
    11401 gtctccgcct tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt
    11461 tgcaaatctt ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc
    11521 ttcaaaagtc atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt
    11581 aaagtacaag tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc
    11641 atatagactg ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc
    11701 taccatttct tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt
    11761 cgatgcaata tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc
    11821 gtcaatcgct tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa
    11881 tgtcttcgca aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa
    11941 caacagacga tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag
    12001 taaaactgtc tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg
    12061 ttgcctcctt ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa
    12121 ttttgtctca ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa
    12181 cgtttttcta tttttaagcg taatttatt
//
LOCUS       DAYYBG010000080        12171 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000080 DAYYBG010000000
VERSION     DAYYBG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.081"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..297)
                     /locus_tag="ACHAUN_002154"
     CDS             complement(133..297)
                     /locus_tag="ACHAUN_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460833.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="ACHAUN_002155"
     CDS             592..1920
                     /locus_tag="ACHAUN_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8460834.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTVVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="ACHAUN_002156"
     CDS             2122..2955
                     /locus_tag="ACHAUN_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8460835.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="ACHAUN_002157"
     CDS             2971..3708
                     /locus_tag="ACHAUN_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8460836.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="ACHAUN_002158"
     CDS             complement(3863..4831)
                     /locus_tag="ACHAUN_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8460837.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="ACHAUN_002159"
     CDS             5063..5896
                     /locus_tag="ACHAUN_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8460838.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="ACHAUN_002160"
     CDS             5930..6769
                     /locus_tag="ACHAUN_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8460839.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="ACHAUN_002161"
     CDS             6795..7355
                     /locus_tag="ACHAUN_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8460840.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="ACHAUN_002162"
     CDS             7554..9275
                     /locus_tag="ACHAUN_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8460841.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="ACHAUN_002163"
     CDS             9440..10582
                     /locus_tag="ACHAUN_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8460842.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="ACHAUN_002164"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="ACHAUN_002164"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8460843.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2042 c         2526 g         3455 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccgttgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DAYYBG010000081        12038 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000081 DAYYBG010000000
VERSION     DAYYBG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.082"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACHAUN_002165"
     CDS             140..460
                     /locus_tag="ACHAUN_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460844.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACHAUN_002166"
     CDS             912..1112
                     /locus_tag="ACHAUN_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8460845.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACHAUN_002167"
     CDS             1259..4048
                     /locus_tag="ACHAUN_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8460846.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACHAUN_002168"
     CDS             4095..4622
                     /locus_tag="ACHAUN_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8460847.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACHAUN_002169"
     CDS             4643..5833
                     /locus_tag="ACHAUN_002169"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8460848.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACHAUN_002170"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACHAUN_002170"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8460849.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACHAUN_002171"
     CDS             7966..8640
                     /locus_tag="ACHAUN_002171"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8460850.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACHAUN_002172"
     CDS             8637..8978
                     /locus_tag="ACHAUN_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460851.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACHAUN_002173"
     CDS             9008..9367
                     /locus_tag="ACHAUN_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8460852.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACHAUN_002174"
     CDS             9627..9749
                     /locus_tag="ACHAUN_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460853.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACHAUN_002175"
     CDS             9891..11975
                     /locus_tag="ACHAUN_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8460854.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4010 a         2108 c         2594 g         3326 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaa
//
LOCUS       DAYYBG010000082        11910 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000082 DAYYBG010000000
VERSION     DAYYBG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.083"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACHAUN_002176"
     CDS             132..344
                     /locus_tag="ACHAUN_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460855.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..742
                     /locus_tag="ACHAUN_002177"
     CDS             506..742
                     /locus_tag="ACHAUN_002177"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM8460856.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITGVSIKS"
     gene            800..1926
                     /locus_tag="ACHAUN_002178"
     CDS             join(800..1028,1028..1926)
                     /locus_tag="ACHAUN_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8460857.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            1971..3158
                     /locus_tag="ACHAUN_002179"
                     /pseudo
     CDS             1971..3158
                     /locus_tag="ACHAUN_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            3278..3478
                     /locus_tag="ACHAUN_002180"
     CDS             3278..3478
                     /locus_tag="ACHAUN_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8460858.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            3554..3751
                     /locus_tag="ACHAUN_002181"
     CDS             3554..3751
                     /locus_tag="ACHAUN_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460859.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(4048..5595)
                     /locus_tag="ACHAUN_002182"
     CDS             complement(4048..5595)
                     /locus_tag="ACHAUN_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFM8460860.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(5679..6482)
                     /locus_tag="ACHAUN_002183"
     CDS             complement(5679..6482)
                     /locus_tag="ACHAUN_002183"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8460861.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(6485..7267)
                     /gene="phnE"
                     /locus_tag="ACHAUN_002184"
     CDS             complement(6485..7267)
                     /gene="phnE"
                     /locus_tag="ACHAUN_002184"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8460862.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(7264..8043)
                     /gene="phnE"
                     /locus_tag="ACHAUN_002185"
     CDS             complement(7264..8043)
                     /gene="phnE"
                     /locus_tag="ACHAUN_002185"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8460863.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(8015..8806)
                     /gene="phnC"
                     /locus_tag="ACHAUN_002186"
     CDS             complement(8015..8806)
                     /gene="phnC"
                     /locus_tag="ACHAUN_002186"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFM8460864.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(8867..9796)
                     /locus_tag="ACHAUN_002187"
     CDS             complement(8867..9796)
                     /locus_tag="ACHAUN_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8460865.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            9991..10674
                     /locus_tag="ACHAUN_002188"
     CDS             9991..10674
                     /locus_tag="ACHAUN_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFM8460866.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            10835..11878
                     /locus_tag="ACHAUN_002189"
     CDS             10835..11878
                     /locus_tag="ACHAUN_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8460867.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         3770 a         2342 c         2318 g         3480 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 tggtgttagt atcaaatctt agacagcttt tcgtagagac aagaataaat taaacttatc
      781 tacgagagga tgaattttta tgacccgata tgaagaaaat tttaaacaaa tgattgttga
      841 actgaatcaa actggacgtt ctgttcgagg gttagcgaaa gaatatggct tatctgaagc
      901 aacgatttac aaatggaaga atttatattt acctgatcag tccacaggac tgactggaaa
      961 agaagtagct gaactgagaa aagaaaatgc tcgtttaaat gaggaacttg aaatcttaaa
     1021 aaaagccgca gccatattct ctcggaaaac ctaaattcgc ttgttcaatt tattgaaaaa
     1081 tggtgtaagg attacactgt ttctttgtta tgtcggttat tagaaatccc tagaagtgtc
     1141 tattattttt ataagaataa accgttgaca gctacagaaa tcagaaataa taagttgaaa
     1201 aagaagattt caacaatttt ttttacgaat aaacaacgct atggtgccac aaagatccat
     1261 caagttttat taaaagaagg gatttcagta tctcttaaac atgtccaaaa gctaatgaaa
     1321 caattaaatt taaggtcgat tgtagttaaa aaatatagac ctcaaaggtc taataaaccg
     1381 atcatttcaa aagagaatct cttaaatcag gacttttcta ctgaaacgat ctgtgagaaa
     1441 tgggcagctg acattacgta cattcctact aagaaaaatg gttggtgtta cttatcttca
     1501 atcatggatt tacacacgaa aaaaattatt agttatacat tttcaaaacg aatgactgtg
     1561 gattgtgtca ttcaaacatt gaataaagca aaaatacatt atcacattcc agaaggaatg
     1621 attctacaca ctgacttggg cagccaatac acagcaagag aagtggaaca atggcttaaa
     1681 accaacaaaa taaggcattc ttatagtaga aaaggaacac cttatgataa tgctggaatc
     1741 gaatctttcc atgcctcatt gaaaaaagaa gaagtctaca cgactagcta ctcagatttt
     1801 gaagaagcaa atcgagcgct atttagctac attgaaggat tttataaccg aaatcgaatc
     1861 catagctcga ttcactatct aaccccacag gaatttgaag aattagcaaa agaaaaaatg
     1921 gcttaaccgt ctctactaaa atgtgtccaa gatattgact caaatccatg atttacaaag
     1981 gaactcttct gtacaagcca gaagagtgtt cccattgtct ttgtgtcgtc ccctctagga
     2041 tcattcgttg ggggacgaca accgttcggt tgttactgaa tgatgcgtct gagtatcgca
     2101 cttatctgga gttgaagaaa caacgattta aatgtaagtc ttgccaaaga acattcgtag
     2161 cggatacctc tgttgctgaa aaacactgtt tcattagtca aaaagttcgt tggtctgtca
     2221 ttgctcgctt aaaagagaat acttccatga cggaaatcgc tcgtcagaaa aatatttcca
     2281 cttcttcggt ctatcgtgtc atgaagcgat tctaccgccc attgaatcct ttcaaacagg
     2341 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
     2401 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
     2461 gcttgcctta tttagaatgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
     2521 aatggattgt tattgatatg tatgcgcctt atgtttcatt ggtgaaaaag cttttcccga
     2581 atgctcagtt gatcatcgac cgtttccaca tcgtccaaca tattggcaga accttcttga
     2641 atcatcgagt gaaagaaacc acggcacggc ttaaaggaac ttcccactct caacgagggt
     2701 tagggaaaaa attgaaacgc tattggaaac tcctacaaaa agaagaagca aagttaaact
     2761 acgaaaaaag ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgtcg
     2821 atcgactgtt atctgcttgt cccaacctgc gccaaggcta tcagttatac caagacattc
     2881 tttatgcggt aaagaaaaga gatcagtcat tatttgagga ctgtttaaac agagaagtca
     2941 gcggtcttcc tgaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
     3001 tccaaaatgc gcttcactac agctattcaa atggaccatt ggaatgttta aacaaccaca
     3061 tcaaagtatt aaaacgcaat gcgtacggct ttcgcagctt ttacaatttc aaactacgga
     3121 tcatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa gagagtttct
     3181 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
     3241 gcctgtattt attactgtcg aggacaatgg catcggcatg attgaagagg aacaaaaaaa
     3301 catttgggat cgctattata aagccgatcc gtcaagtaaa aatacaaaga taggcgagtc
     3361 aggtcttgga ttatcaatcg tgaaggaact agtgcgcctg caccacggtt caattgaagt
     3421 gaccagccga aaaaatgaag gaacgacctt cctgatacaa tttccaaaag aaaaataaaa
     3481 ttaccagtaa aaaaccgtgg agagatagct gctattgatt tttgctatgt cttttctttg
     3541 agaggtatcc ggtatggtgg agtgtctgaa aaataaaaaa atggaggcca attttgtgaa
     3601 agctgttatt tctggaagta ttgttttcca agaagaatat cgagatatgg aaaaagattt
     3661 cagtaaaaaa ggcattgaat taattgatta tcctcgctca tgtccgatcc tagacaaaga
     3721 gtatccagaa atcttgacga ctttttttta aatatcgcag cagcggattt attttttgta
     3781 tttaatcaag atcggaaagg ggtattaggc tatattggtg ccgcaacttt ttcagaaatg
     3841 aagtattgtc tgatgcaaaa tcttattcat ggaaaaagga tcaaaatagt cttatttaag
     3901 gaaccatcag aggaaaattt tttgttttga tgaggtagat cgctggctaa agaatggctg
     3961 gatggaaatc agacagccta acaatttggc ataacaaagg tgtgggcaaa aaactgctca
     4021 cacctttttg aattttttat ttatatacta caaaatcact tgaaaattat gattcacttt
     4081 tgcttcgatg cgtgggtgaa tattcaaata gtcggcaatt aattcggtca tgtcgatttg
     4141 gatttctcgg acaattttct ccgcaccaaa catcgcataa tttcctccgc cgattgcccg
     4201 atattgattt gtcaccactt ccagcatttg ttgcggaaga acggggtgac ctttaaaacg
     4261 aagttcggta atccgttgtc caacaggctg tttcatatca atagtgtact gaatcccttc
     4321 atacatgtca taattgtagt actggggctt aggctgaata aaggctggat tgaatacgat
     4381 ttggttattt tctacagcta aataatttgc ggtctgctcc aacgccgctc tcaaatctgc
     4441 accatttatt tgaatcactg ccagagtatt cggataaata tagttggtga tgacgtcccg
     4501 catcgtgatc gtctcaccaa agccttttcc ttcattattg aataacgcag ttccagaaat
     4561 ctctgagccg ctggcaaata attgtacacg attaataaac tctacatagg gatgttcctc
     4621 aagacgagcc tgcatcggat cattaatacg catatttccg ttcactttac ctaagggttg
     4681 atccaaccat ttctctagtt tttcttctac cggtacgatc gctgcaagaa tttctggatc
     4741 aggctctgat ttttcaactg agtgtaaagc aacctggtga tctgtaacaa ccacttgccc
     4801 ctcttttttc tcgacggtaa gacgaatttc accgatgaac tcgccacgat agcctggttg
     4861 aacaacggga actccatgaa ccgtttcagc aattttccgg tgctgatgac cggtgaaaag
     4921 ggcgtcaatg ccttcaacct gctgcaatag ctcgtagcct tcattctctc cagttagcgc
     4981 ttctgtgtcc tcaccggtta acaagtcttt ttcaaatccg ccgtggtaac taacgattac
     5041 caagtctgct tgtttcctta atagaggaac atattctttt gctgtttcga cgatactctt
     5101 aaacgtaatt cctgctaagg tttctggctt ttcccagtga ggaatgtatt gtgtcgttaa
     5161 tcccaagata gcaattttta cacccacctt ttcgataatc ttataggcgt ttccaaagta
     5221 tggttggtca tttttgttca ccatattggc tcctaatata ggcgcttgat aattcttaat
     5281 cgcttcctcg agataatcca caccatagtt aaactcatga tttcccaaca cttgaacatc
     5341 atagctcatt tggttaatca agtctgtaag ctctttggct gaaccatatt ctttagcaag
     5401 ataataactc aaaggagacc cttggatgaa atcaccattt tcgatcgtaa ttaccggtcc
     5461 atctgctttt gcccgtaatt ccttcagttt cgacgatact ttagccgttc ctaaggccag
     5521 atcggttctg ccggaatagt ctgttggtga aacatagcca tgcatatcac ttgtctctaa
     5581 aaacgtaatg ttcattctgc gtgctccttt agctgactca aaatagaaaa accgaagcgt
     5641 attcagctgt tcggtctctt tttttgattt ataatgcctt aatccttcat ttgaagtgtt
     5701 tttaaaatgt tgcctggaat aaagttttct gttgtctggc gaacaatcct tccttgatcg
     5761 agatgataaa ggttataccc atgacgttct tcaaagcgta cgaagccatc gccttcatct
     5821 tcaccaacaa aggacatact cgatacagta aaatagggga tcccattaat ttcatcagta
     5881 aatgtgtgat gggtatgacc gtttaggata cagcgaatat cgtaaggtgc gattagcttc
     5941 aagaaacgtt cggtaccagg ccattttggt atactgcttt ggtgagacag tagatgatga
     6001 tgggtgacca agaaaatcgg ttggtctttt tcttgctcta aggctttttt cagccattga
     6061 aattgctgat cgtccactat accatctgcg tatccatagc ttgaattatc aaaggaaatc
     6121 actgtaaact caggatattt gactatctgg ttgtacgggg cagaagaggc ggcttcatcg
     6181 caccagccta cccaaaaatg ttctttgata tcgtgattgc ctagagtaac aagaatcgtc
     6241 gtttctccta gcgcattttc cagccaagtc ttcaagtatc gataatcgtc tatttcgcca
     6301 tcttcggtta aatctccgct gataatcaca aggtccaagg gtgtttgctg caggagatga
     6361 tgaatgcatt gctccagagg gataagggga ttctgcatcg tagccagcat tcccttatag
     6421 ccttcttcac aagcctcata ctctctgcga aagtgtatat ctgatatatg caatatattc
     6481 attcttatct caatctcttt cttataattc cggttatttg atctaaaata aacacggtta
     6541 cgactacgcc aatcaaaata atactcactt tgctccaaga ccgagtttga atcgcgaata
     6601 tcaatggagc accgattccg ccagcgccaa ctaagcctaa ggtagccgca ctacgaaccg
     6661 ctatttcaaa atgatttaat gctaaagaac aataaatcgg catcaaatag gggatgcgaa
     6721 caaagaaaat cgtttgccaa aaatttgctc ctaccgcatg catttcttca acaagtcgct
     6781 catccatcgc ttccatttct tctgtaaaaa gtttgccaag cattccgatc tgatggacgc
     6841 caatcgccat aacaccagca aaggggccag gtccgacgac tttcacaaaa ataattgcaa
     6901 aaactagttc aggaaaagct cgtaaaacat tacagataag cttgccaatt tttgttaccc
     6961 atggatgtgc ttgccagagg ttaaccgcac tgatcaaggt aatgggaatc gctaataccg
     7021 tagcgatcat tgtccctaaa caggcgattc caatggttaa taatagcaaa ctaatcaaat
     7081 cctcgccgct gccatcatat agaaactgcc aatcaggctg acctaaccct ctcaaaacgt
     7141 ccattcccat ggaaggagag aaggtgctta atccagagta atccagagta gcggccgaca
     7201 aaataacgag aatcactaaa gcaactaatg ataaaagggt tcttctgttc atttcacgaa
     7261 gcatcataac agggacctcc tgacacgttc acttaattta tcaacgatcg cgacagttac
     7321 taggatcgtt agaataatga ttgaaactcg ttcataattc aaaagcgcaa gtgaggaatt
     7381 tagaataacg ccaataccac cagctccgac atatccaagg acagtagaag cacgaacatt
     7441 cacctcaaac gcgtaaaaag catagctcgc aaactgactc aaaacttgtg gagcgatcga
     7501 ccaaatagcg atttgcattt tatttgctcc aacagccgca gcggcttcaa tcgggccaaa
     7561 gtcaatggtt tcaattgctt gaaacattaa ttgtgacacc ataccaaaag taaagacagc
     7621 gatagtcaac acgccagtcg cttcaccaat tccaaacaca gcaacaaaaa gcgcggctaa
     7681 aagcagggta gggatggtcc gtactaaacc taaaagaaaa cgaaagatgg tagaaagaag
     7741 acggttttct gtcactacag tggtcgctaa aaaactaaca gggatcgcta aaattaagcc
     7801 aatgaccgtt ccaagcaaag acatttgaag tgttttcacc ataggctcga ctaatttagg
     7861 taaatagcta aagtctggat gaatgaagcg tgataaaaag acagtcattt gattggagtt
     7921 ttgaaaaacc tgagaaaaat cagctgacgt gatccaggca cttccttgaa aacaaacaat
     7981 taagaacaga agaataagca ggtgtttata ccatttaaag tgaacggttt ctttcaagct
     8041 catcgatttc gcctgccttt tcttcaaaga ttgcattgcc ataaatttgc tctaagcgtt
     8101 catcggttaa gtcgtctgga cgaccatcaa agacaacctg agcagctttt aacgcgagca
     8161 cacgcgtaga aaactcacga gccagaggga ctgaatgcag attgacaatg actgttttat
     8221 ccatcgtttg attgatattt tttaggtcca tcataacctt ttgagtcgtg attggatcta
     8281 atgatgcaac gggttcatcc gctaaaataa tgggggcttg ctgcacgagt gctcgcgcaa
     8341 tggatactcg ctgttgttgc ccaccactga gctcatcact gcgtgtctgc aatttatcag
     8401 acaaaccaac tttttccagt gcttcctttg tctgctggta gtcttccttc gtaaaaagac
     8461 ctaaaatact tttccaagtg gagtaatagc ccaaacgtcc agatagcaca tttttctgaa
     8521 ccgtactacg ctttaccaga ttgaagcttt gcgagattag accaatctgg cggcgaatct
     8581 gtcgcagctc ttttttattc gcttttgtaa tagaggttcc attaatgcga atatctcctt
     8641 tggtggtttc tgccaagcga ttgatactgc gcagcagagt gctctttcca gcaccactca
     8701 accctatgat ggaaacaaat tctccatctt caattgttaa gtcaatatcc ttcaagccaa
     8761 caacgccatt gggatagacc ttcgaaacat tgtcaaaaat aatcattatc cttctccatt
     8821 tttttaagtg taagcaaaaa aaagcttgct gacacttact gatgttttat tctgctgctt
     8881 ttttcgtgta gtctctgatc gtgtcatagg cagaatcagc agcttcttca tagccctgat
     8941 gcccccacaa agccattgct tctttgccat ctttatcatc cttcatattt aagaaggtct
     9001 ctttaatttt cgcttttaaa tcttttgaca tgtcaggctg tacagcgatt gcgtcattcg
     9061 gaatatcgcc ttttgtcagg tacaggactt ttaaatcctt aaataaatca ttatctggga
     9121 aattcgaagc aaaaacattt cgcgcgcctt caaaaacaaa cgccgcatcc atttgcttgt
     9181 tcaacactgc ggtaatctca ctcggaatat cattcacagt ggtcatcttt gtatctgcag
     9241 taggatcaat cccagcatct ttcaattcag cgacaggata gatgtagccg ctagcagagt
     9301 taggacttaa ggttgcgata ttttttcctt ttaaatcagt taacttattc aatccgctat
     9361 cggcacgaac caatacttct cctttaaagg tgttcgtaag tttgtccttt aatggcaagc
     9421 cagtttcttg atcataatcg ccgagctgag aagtcaggat tgcatctgca gctccagcat
     9481 cctttgcttg aacataggct gcaggcggca taattccgac atcgacttga ccagaagaca
     9541 tcgcttcgac gatcgttgaa taatcagtag ctaacgtcac ttctacctca cgatctaact
     9601 ttttcgataa atacttagca aatggttttg cttttgcctc catactacca tcattgtttg
     9661 ttggaacgaa ttgaacaact aatttttctt tgctctcttt ttctgagctg ctacttgtat
     9721 tcccagaacc gcaggccgcc aaagtggtca ataacatgac tccgatgaac ccttttgcta
     9781 actgtttgaa ctgcattttt ctcctcctgt tggatataat ttctaatacg tctaaatcat
     9841 agcagggaat caaaatcaat gaagtgacgc acgtcctatt ttacacaact tttacataca
     9901 atttacaaac aattataaat aattattcgt attgagccga aaaacaatct aagctataat
     9961 aaagaaaaac ttcttagagg tgacgatttc atgaaaaaaa tccatgatac gaagcaatta
    10021 actgcttacg ttgagaagca caacctgcag gattatatgg attcagatat attggcaatg
    10081 gcttctttac ttttttttga aaaggaagag cacctcattc aaacgggaaa aacctcagat
    10141 tatttgtatt ttttagtgga tgggacggta atggtgtact cgtattcttc tgatacacaa
    10201 aatatttgca tcgattacgc aaagcccgct acaccactcg gcgaagcctc ttcattgtgg
    10261 gaattatcgc cgaaaagcag cgttaaagca gtgactaagt gcatctgtat cagcattccg
    10321 ctaaatcaat atcgaacaca gcttcaaaaa gatgttcgct ttttgcagaa tatttgccag
    10381 ttactaagtt atcgtttaaa ttccgggata aatttggcga actcactgac agaacctgtt
    10441 gaaactcgtt tagcaaagtt tattttgacc catcacgaaa acgaacgttt ctcatttcgg
    10501 ttaaccacct gcgcctcaat cttaaatgtc agttatcgac atcttttacg aacaatcacg
    10561 aattttcgtg aggagaatat tatcgaaaag aaaaagaatt attacttgat tcatgatatc
    10621 gaagctttag aggacttagc tgagaatata agtagtccag taaaagcaga ataacagcta
    10681 taaaaaaaca gcagtcagaa gtgattgctg gttttttgat agtggagaaa ttgggaaacg
    10741 gaaaatcttc gttgaagagt agtgttctat atattggtat gctatacttt taaaagttca
    10801 atgtgtcaag acacataaaa aggggagaaa aattttgaag aaaatacttg tttcattgtt
    10861 gtttttggtg tcattagtaa cgttagtcgg ctgtagtcag tctcaggggg agaagcaaag
    10921 tgaaaaagag aaaagtaaaa tcgctttggt tcttggattt ggcggggttg atgaccgctc
    10981 cttcaatcaa gctggctggg aaggattaga ggcttgggga aaagagaaaa agctgacagc
    11041 aaaaaaagat tttacgtata tagaaacgaa caatccagag gataacagta ccaatatcga
    11101 tcaggcaatc agaaacggct ttaccaccat tattagcttg agctacttgc aagcagagga
    11161 actggaaaat gctgctaaag ataatccaaa gttgaaattt gccttggtag atggtgagat
    11221 tactgggaaa aagaatatct catcagcaac ctttaaagat caagaaagtg cgtatttagc
    11281 aggactagct gccgcatata cgacaaaaag tgatcaagta ggctttattg gtggaatgga
    11341 agggcccgtg atccaacgtt tccaaagcgg atttgaagct ggcgttaagg caggggccac
    11401 agcactgaac aaggaaatta ctgttaacgt acaatatgct gcatcctatg attcgctaga
    11461 taaaggaaaa gctattgctg cttctatgta caaagaggga gtcgatattc tttatcaggc
    11521 ggcaggaagt actggagctg gggtgttcca agaagcaaaa gcccgcaatg aaaacgacta
    11581 tagcagaaaa gtatgggtga ttggtgtaga tcgcgatcaa gcggatgaag gggattataa
    11641 aatggagaac ggcgagacag ggaatttcac gttgacttct acaaagaaaa atgtcggtgc
    11701 agaagtcaaa gaatttgcag aaacttctca taacgataaa tttcctggcg gcaagcattt
    11761 gacctttggt ttgaaagaag aaggggtaga tctgacaaaa ggacaattat cagaggaagc
    11821 ctatcaggca gtttcagacg caaaagagaa aatcatcagt ggtgatttca gactgtagac
    11881 aaagtccatt ttatggatga tgtctacagt
//
LOCUS       DAYYBG010000083        11732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000083 DAYYBG010000000
VERSION     DAYYBG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.084"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..631)
                     /locus_tag="ACHAUN_002190"
     CDS             complement(146..631)
                     /locus_tag="ACHAUN_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8460868.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    721..1066
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1111..1752
                     /locus_tag="ACHAUN_002191"
     CDS             1111..1752
                     /locus_tag="ACHAUN_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8460869.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1771..2406
                     /locus_tag="ACHAUN_002192"
     CDS             1771..2406
                     /locus_tag="ACHAUN_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8460870.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2424..3239
                     /locus_tag="ACHAUN_002193"
     CDS             2424..3239
                     /locus_tag="ACHAUN_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8460871.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3558..4994
                     /locus_tag="ACHAUN_002194"
     CDS             3558..4994
                     /locus_tag="ACHAUN_002194"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8460872.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5183..7630
                     /locus_tag="ACHAUN_002195"
     CDS             5183..7630
                     /locus_tag="ACHAUN_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8460873.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7860..8927
                     /gene="rlmN"
                     /locus_tag="ACHAUN_002196"
     CDS             7860..8927
                     /gene="rlmN"
                     /locus_tag="ACHAUN_002196"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8460874.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8917..9123
                     /locus_tag="ACHAUN_002197"
     CDS             8917..9123
                     /locus_tag="ACHAUN_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460875.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9120..10223)
                     /locus_tag="ACHAUN_002198"
     CDS             complement(9120..10223)
                     /locus_tag="ACHAUN_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8460876.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10776..11324
                     /gene="aac(6')"
                     /locus_tag="ACHAUN_002199"
     CDS             10776..11324
                     /gene="aac(6')"
                     /locus_tag="ACHAUN_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8460877.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(11572..11691)
                     /locus_tag="ACHAUN_002200"
     CDS             complement(11572..11691)
                     /locus_tag="ACHAUN_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8460878.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         3888 a         1978 c         2522 g         3344 t
ORIGIN      
        1 ctaactatgg aagaatactc gtattagttt tcagcgttca tactagttgt tacattgttt
       61 gattggccaa aagaatttct gatatatttt gtgctaaata aagaaaaaga cgcttgaatt
      121 gggaaataag aaaactactc tatcattaca aattttcata aactggcaga tgttctaagt
      181 aaacaagtat cttttcaaag tcggaaaaat ccataatcaa cgtagtggta ttgatatttg
      241 gatgaaagcc tacggtatct tgacgatcaa tagcagcatc gatcacgact tggatttttt
      301 tctctttgtc gtgcatcaat gctaatggag tcactgtacc tgctggaacg cctaggaggt
      361 caggaagctg ttcttcagaa acaaaagata agcgcttttc ttttaattgt tcagctagtt
      421 gttttaaatc ggcgtgcttt tcatcaggca agatgacaag atagatttgt tttccttttc
      481 ttgatttcaa taatagattt tttacttgcg gaccaggtag ggaaaaaggg acatttttga
      541 ctgacgtaat cgcaggatga tcgacttttt gaaaagagat acccagtttt tttagtagtt
      601 cataagcttc ttgttcggtt gcacgactca tcgcgcatca ccctcctttt tttattagtg
      661 tatcataaaa aagagttgac aaatgaaaaa taatcatgta aattacacgt aaagttaaaa
      721 caaatgacat cgaaaagaag agtagccgtt aggtgttttt agagagtctc tggttggtgg
      781 aaagaggcag cagttaaccg tgaaacacat cttggagtcg attttctgaa gcgtatagta
      841 ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta gatgaccaga taaggctggc
      901 ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg attaacgtcc tttgatcttc
      961 ggatcaaagg actttttgtt ttcatctgaa cggcaacgac tgtgtgaggt tttttctgca
     1021 aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc ctcttggcat ttgccaagag
     1081 gattttttta ttttaaagga ggaaaagaag atggagtatt tgattgaagt tatgccgcaa
     1141 ttattagtag gagcactcac aacattgaaa ttatttttat ttaccttgat tggctcatta
     1201 ccattaggga ttgttttagc attaggattg tctttgcatt ttaagccgct acgctacttg
     1261 cttcaaacat atgtatgggt tatgcgggga acaccgctgt tgttgcaact gatttttatt
     1321 ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt tcgaagcagc actcattgcc
     1381 tttatcttaa actatgcagc ttattttgca gagatctttc gtggagggat acaatcgatt
     1441 cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa gtccctttca aacggtgacg
     1501 aaaatcgtca tcccacaagt gacaaagatt gttttacctt ctgtagggaa tgaagttatc
     1561 aacttggtca aagattcttc cttgatctat atattaggtt taggtgacgt aatgcgtgct
     1621 gggaaaatcg cgatggaacg cgacgcgact ttactgcctt tgctaggcgt ggcaatcatc
     1681 tatcttacat tgactggtat tctaacaatc ataatgaaac aagttgaagg aaaattaaat
     1741 tattaccgct aactgatagg aggaagaaat atgattgaat tgaagaaagt gaacaaagca
     1801 tttggtgaaa aaaaaataat caaagatttg aatctaatca ttccagatgg gaaaacatta
     1861 gctatcgtag gtccttccgg tggcggtaaa accacgcttt tacgtatctt ggctggctta
     1921 gaaagttctg acagcggaga attttattta gacggtgtac cgttcaaccc attcgagctt
     1981 aaaatgaaag aacaagttgt aggagtcgta ttccaagact tccagctatt tccacatctg
     2041 aatatttttg aaaacattac cttagcacca aaaatggtat tgaaacagac aaaagaagaa
     2101 tacaccaaaa aagcagaaga attagcggct tcattaggga ttagtgattt attgaatatc
     2161 tatccttatc agctttctgg cggacaaaaa cagcgggtag ctattgccag agcattggct
     2221 atgaatccac gggtattggc ttatgatgaa ccaacaagtg ctttagatcc tgaattacgc
     2281 cagcaagtcg agcgtttgat cctatcattg aaagaagatg gggtaaccca aattattgtc
     2341 acccatgatt tagtatttgc agaaaatgtc ggtgatcaat tactaaaagt ttccccaatt
     2401 cattaagaaa ggagaagata gagatgaaaa aaaccagttt tctattcgct ttgatcgcag
     2461 gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc agcagataat tgggcgacta
     2521 tcaacgaaga gaaaaaaatc gttataggtt tagacgatac atttgttcct atgggattta
     2581 gagataaaga cgggaagctc acaggatttg atattgattt agccaaagca gtctttgaac
     2641 aatatgggat tacggtagac tttcagccca ttgactggtc aatgaaggaa tttgaactga
     2701 acaatggaac gatcgatttg atatggaacg gctattccaa aacgccggca agagaaaaga
     2761 aagtgcaatt tacaaaacct tatatggaaa acgatcaagt gctgatcaca cctaaagaat
     2821 caggaatcac aaactttgga caaatgaata ataagagatt aggtgcgcaa aatgggtcat
     2881 ctggttatga tgtatttacg aaacaaccag aagtattaaa agagatagtc aaaaaccaag
     2941 atgctgtttt gtatgatagt tttaatgaag cattgatcga tctgaaaagc agacgggtcg
     3001 atggtctgct gatggataaa gtatacgcag actattattt gaaacaaaga aacgaactag
     3061 aagacttcaa cataacaaaa ggttcttatg agagtgagga ttttgcagtt ggtgcacgca
     3121 agtcagatag tacattagtg aaaaaaatca atgaagccct agatcaacta gagaaaacag
     3181 gtgaattcca aaaaatatcc gataaatggt ttggcgaaga tgttacgccg agaaattaac
     3241 aaatagggga ctgtgacaaa agacgtgtca cagtcccttt ttccgaataa acagtgttct
     3301 cctgccaact ccttgtgact cggagctgaa tggcaggctc acaacctctt ggtaaacggt
     3361 gttcaaaagc cagtttttcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt
     3421 tcacaaaatt tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa
     3481 ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat
     3541 aggaaaggaa gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt
     3601 ggggctctgc ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag
     3661 aatcagtctg ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg
     3721 atttggcagt agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag
     3781 gtctgaaagc ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag
     3841 aagtcaatca agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg
     3901 atattgaacc tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat
     3961 ataatggttg ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat
     4021 ttgaggcatt ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca
     4081 cttcattagg ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt
     4141 atgaagttaa ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt
     4201 tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca
     4261 gtaatcctga aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga
     4321 tggatgtcta tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag
     4381 gatgggcacc aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt
     4441 ttcttgggat caattactat caaacggcga cgaatgcatt caatccatta aacggagttg
     4501 gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg
     4561 gaatgtataa aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg
     4621 acccagaagg attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac
     4681 tgattacgga aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg
     4741 acgactatcg aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg
     4801 atggagcaga agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga
     4861 acggttatca aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat
     4921 ctttggaacg atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg
     4981 gagagaacct ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct
     5041 tttcttgata aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag
     5101 aaagcgtcag ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac
     5161 ttgatgaaat gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa
     5221 gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg
     5281 gtttgccttg attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt
     5341 gattgcaaac ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt
     5401 agccgtatta ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg
     5461 acgctggttt ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt
     5521 atttggcaaa ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc
     5581 cagtgatata aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat
     5641 cctttatcat tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct
     5701 attactggca ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg
     5761 tatccaatca attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc
     5821 tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag
     5881 gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc
     5941 ggtgagccag caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc
     6001 ggagcagcta agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct
     6061 agagaaacca gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga
     6121 gttgccagag gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca
     6181 agactatgaa ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga
     6241 tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg
     6301 tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca
     6361 gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc
     6421 tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat
     6481 tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc
     6541 aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca
     6601 aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa
     6661 gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt
     6721 aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca
     6781 tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt
     6841 acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg
     6901 ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc
     6961 attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt
     7021 tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct
     7081 agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc
     7141 cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg
     7201 ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc
     7261 gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt
     7321 tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac
     7381 gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt
     7441 tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg
     7501 agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag
     7561 caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa
     7621 tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg
     7681 catgatgata ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc
     7741 ttgtcttttt agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat
     7801 taaggaaatg aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt
     7861 tggaaaaaca gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa
     7921 acggcgagaa aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa
     7981 gtaatttcac agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt
     8041 ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat
     8101 atttgtttga actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg
     8161 gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa
     8221 gcgggctgct caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga
     8281 tggtccaaca ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa
     8341 tggggatcgg tgagccattt gataattacg acaatgtaat ggactttttg catatcatca
     8401 atgatgccaa aggattggca attggtgctc ggcacattac agtttccact agtggattag
     8461 cacacaaaat caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc
     8521 acgcgccgaa taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg
     8581 agaaattgat ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg
     8641 aatatatcat gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc
     8701 tattaaaaga taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg
     8761 aacatgatca atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga
     8821 agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt
     8881 gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc
     8941 gttttttcaa ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat
     9001 aagagaaaag attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc
     9061 aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt
     9121 taactgattt tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca
     9181 agaatggcct gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt
     9241 gttgccatgg ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg
     9301 gttaacgata ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg
     9361 aactgctgat ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg
     9421 tatccctcca ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa
     9481 ttatagttat tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg
     9541 acttgttcaa ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca
     9601 taagtcaaga gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag
     9661 cctagatcat cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca
     9721 atacctgccc ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg
     9781 tgtacaggtg gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg
     9841 acacataccg cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt
     9901 ttagctagtt tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca
     9961 aacaggacgt ctgctttttg gacactttca tgctggatca actgttcaat atttccataa
    10021 gttgaattac ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact
    10081 tttattttgt tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc
    10141 ccatattctt tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt
    10201 ggtaagaaaa cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa
    10261 ttttaaagag tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag
    10321 gataagtgat gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac
    10381 gaacaagtac aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct
    10441 accgattccc tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat
    10501 gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat
    10561 atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt
    10621 ttgaataaag tgtttctgta taattttttt attttataag taagataaat aatgaaagaa
    10681 tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg
    10741 actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta
    10801 ataatccagt attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat
    10861 atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag
    10921 cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca
    10981 caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta
    11041 ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt
    11101 taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata
    11161 catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa
    11221 aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata
    11281 tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga
    11341 cagaagtaat cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt
    11401 gtgaaattcc aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt
    11461 gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt gaaaatggct ttctacattt
    11521 ttgcataaat ttggcttaat accgagaaac tggcttttga acaccgttta ttcagataag
    11581 ggactgtgac aagacttttg tcacagtttc tttttgttta ttctttttta tctttgtatg
    11641 gcaataatag acaatataac gaaaattatt taatggcttt tggaagacca tggaaaacct
    11701 tgtacaaagc tattttttct gaaaaattcc tt
//
LOCUS       DAYYBG010000084        11556 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000084 DAYYBG010000000
VERSION     DAYYBG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.085"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..1482)
                     /locus_tag="ACHAUN_002201"
     CDS             complement(364..1482)
                     /locus_tag="ACHAUN_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460879.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1715..1837
                     /locus_tag="ACHAUN_002202"
     CDS             1715..1837
                     /locus_tag="ACHAUN_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460880.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1953..3260
                     /locus_tag="ACHAUN_002203"
     CDS             1953..3260
                     /locus_tag="ACHAUN_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8460881.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="ACHAUN_002204"
     CDS             3379..4578
                     /locus_tag="ACHAUN_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8460882.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="ACHAUN_002205"
     CDS             complement(4605..5873)
                     /locus_tag="ACHAUN_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8460883.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5928..5994)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6036..6682)
                     /locus_tag="ACHAUN_002206"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="ACHAUN_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="ACHAUN_002207"
     CDS             complement(7090..7593)
                     /locus_tag="ACHAUN_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8460884.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="ACHAUN_002208"
     CDS             complement(7716..8480)
                     /locus_tag="ACHAUN_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8460885.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="ACHAUN_002209"
     CDS             8588..8863
                     /locus_tag="ACHAUN_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8460886.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="ACHAUN_002210"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="ACHAUN_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8460887.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10609)
                     /locus_tag="ACHAUN_002211"
                     /pseudo
     CDS             complement(10430..10609)
                     /locus_tag="ACHAUN_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11435..>11556
                     /locus_tag="ACHAUN_002212"
     CDS             11435..>11556
                     /locus_tag="ACHAUN_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8460888.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT         3493 a         2423 c         2037 g         3603 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaataacg
    11341 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    11401 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    11461 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    11521 ccgaatggga cgtgctatac aaactattta taatgt
//
LOCUS       DAYYBG010000085        11229 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000085 DAYYBG010000000
VERSION     DAYYBG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.086"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHAUN_002213"
     CDS             136..309
                     /locus_tag="ACHAUN_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460889.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACHAUN_002214"
     CDS             949..1422
                     /locus_tag="ACHAUN_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8460890.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACHAUN_002215"
     CDS             1431..1658
                     /locus_tag="ACHAUN_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8460891.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACHAUN_002216"
     CDS             2294..2665
                     /locus_tag="ACHAUN_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460892.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ACHAUN_002217"
     CDS             complement(2921..3163)
                     /locus_tag="ACHAUN_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8460893.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ACHAUN_002218"
     CDS             complement(3195..4097)
                     /locus_tag="ACHAUN_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8460894.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ACHAUN_002219"
     CDS             complement(4110..4298)
                     /locus_tag="ACHAUN_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8460895.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACHAUN_002220"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACHAUN_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8460896.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ACHAUN_002221"
     CDS             complement(4913..5806)
                     /locus_tag="ACHAUN_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8460897.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="ACHAUN_002222"
     CDS             complement(5884..6807)
                     /locus_tag="ACHAUN_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8460898.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="ACHAUN_002223"
     CDS             complement(6856..7206)
                     /locus_tag="ACHAUN_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8460899.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ACHAUN_002224"
     CDS             complement(7239..8141)
                     /locus_tag="ACHAUN_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8460900.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ACHAUN_002225"
     CDS             complement(8134..8991)
                     /locus_tag="ACHAUN_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8460901.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="ACHAUN_002226"
     CDS             9325..10134
                     /locus_tag="ACHAUN_002226"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8460902.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="ACHAUN_002227"
     CDS             10174..10671
                     /locus_tag="ACHAUN_002227"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8460903.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="ACHAUN_002228"
     tRNA            complement(11056..11129)
                     /locus_tag="ACHAUN_002228"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
BASE COUNT         3295 a         2098 c         1879 g         3957 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttat
//
LOCUS       DAYYBG010000086        11228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000086 DAYYBG010000000
VERSION     DAYYBG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.087"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..1449
                     /gene="lysA"
                     /locus_tag="ACHAUN_002229"
     CDS             190..1449
                     /gene="lysA"
                     /locus_tag="ACHAUN_002229"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8460904.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1436..2236
                     /gene="dapF"
                     /locus_tag="ACHAUN_002230"
     CDS             1436..2236
                     /gene="dapF"
                     /locus_tag="ACHAUN_002230"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8460905.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2239..3120
                     /gene="dapA"
                     /locus_tag="ACHAUN_002231"
     CDS             2239..3120
                     /gene="dapA"
                     /locus_tag="ACHAUN_002231"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8460906.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3117..3833
                     /gene="dapB"
                     /locus_tag="ACHAUN_002232"
     CDS             3117..3833
                     /gene="dapB"
                     /locus_tag="ACHAUN_002232"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8460907.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3839..4534
                     /gene="dapD"
                     /locus_tag="ACHAUN_002233"
     CDS             3839..4534
                     /gene="dapD"
                     /locus_tag="ACHAUN_002233"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8460908.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4550..5551
                     /locus_tag="ACHAUN_002234"
     CDS             4550..5551
                     /locus_tag="ACHAUN_002234"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8460909.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5548..6909
                     /locus_tag="ACHAUN_002235"
     CDS             5548..6909
                     /locus_tag="ACHAUN_002235"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8460910.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6869..8020
                     /locus_tag="ACHAUN_002236"
     CDS             6869..8020
                     /locus_tag="ACHAUN_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8460911.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7995..9164
                     /locus_tag="ACHAUN_002237"
     CDS             7995..9164
                     /locus_tag="ACHAUN_002237"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8460912.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9339..10718
                     /locus_tag="ACHAUN_002238"
     CDS             9339..10718
                     /locus_tag="ACHAUN_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8460913.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10999..11228
                     /locus_tag="ACHAUN_002239"
                     /pseudo
     CDS             10999..11228
                     /locus_tag="ACHAUN_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3739 a         1955 c         2503 g         3031 t
ORIGIN      
        1 tataaatagc ttctaatagt tcgggtattt tgtttttata accattttta ttcttatttt
       61 taaaaaaata tttcgaaata tactagacaa ttcagaaaat tttgtttaat attattccaa
      121 taaaattcaa atctttatca aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg
      181 aatgaaaaaa tgacagaatt gataattggc ggagtcacag caagcacatt ggcagaaaag
      241 tataagacac ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat gctgtcattc
      301 aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc tttacgcctc taaagcattc
      361 caaacgattg agatgctgca tttagttcgt gaatatggat tcgggctaga tgttgtcagt
      421 ggtggggaaa tctataccgc tttgcaagct gattttccta gagatactat ctacttccat
      481 ggtaacaaca agacgccaga ggaactgatc tatgcacttg aaaatgattt gttgcatatt
      541 gttgccgata atttgatgga agttgaatta ttagcagaat taagccaacg ctatttaaaa
      601 aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag cacatactca tgaatatatc
      661 gtcactgcgc atattgattc aaaattcggc atgagctatg aaagtaaaga gtgccaacaa
      721 gcccttcagt tgataaaaga aagcagctat ctagagttag aaggatttca tgctcatatc
      781 gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt gacgaattac
      841 ttaaccgatt ttgaccaacc tctttccttg aatcttggcg gagggtttgg gattcgttat
      901 acacaaaatg atcagccgat cccagtcgaa gaggcactaa aaaaactgat tgaatatacg
      961 gaagaagctt tggaaaaacg aaaactgacc attcgtcaat tactgatcga accgggtaga
     1021 agtattgttg gagaagctgg tacaacacta tacacgattg gtttcatcaa aaagacacca
     1081 cataaaaaat attactttgt agatggcggg atgacagata atatccgtcc agctctgtat
     1141 caggcagaat acgattgtga tttagcagca aaactgtcgg aagaaaaaac agagaaagtg
     1201 acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg
     1261 aaagcagaat cgggtgatct tttagctgtt tattccacgg gagcttatgg atactcgatg
     1321 agcagcaact acaatcgagc gacacgacca gcagttgttt ttgtcaataa tggccaatca
     1381 agactgattg ttcgcagaca agcattttct gatctactaa ggggggaagt ttcctatgat
     1441 ccttcataaa tataatggct gcggaaatga ttttatctta ttagattacg cagaagacac
     1501 agattatagt caattagccg cttctttatg cacaaaagaa aaatatgata tagacggatt
     1561 gatcgccgta aaaatcgatc cgttggaaat gatctattac aacaaagatg gcagccgcgc
     1621 gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc agaacatgat
     1681 gaatgaacag gaattcaacg tagagacatt agcaggcaca agaaaaatta cggtgttgga
     1741 tccctcacct ttttattgca gcgtagatat gggagtgcca gatgattctc cggaaaaaat
     1801 caaagctaat caaacttatc caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt
     1861 aacgatcact agtttatttt taggaacgat tcataccgtt gtttttgttg aaaatgcgcg
     1921 tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat catcctttgt tcaaagaaaa
     1981 aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga
     2041 aagaggagta ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc
     2101 taaggaaact gggaaaattt cagcagatca ggtagttgtt cacttggaac aaggagactt
     2161 gatgatctct ggtgaggatg cgatacaaat ggctggccca gcagttcatg aatggacaag
     2221 aatattggag gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat
     2281 gaagagggat cagtagatta tgctggtttg gaaaaattga tccaattcca tttagatgaa
     2341 caaacagatg gattattagt tttaggtaca acaggagaat cttctacgct cacacaatct
     2401 gaggaggagc agatccttca actgacagta aagaaagtgg ctggccgtgt gccggtgatt
     2461 gcaggagctg gaacgaataa tacgaaagaa acgattgaaa aagcgaagca ttttgcttct
     2521 ttgggggcag acgcactttt ggtcatcacc ccttactaca acaaaacaag tgatgcagga
     2581 ctagctgctc attttacagc aattgcagaa gcctcaccga ttccgcttat tttgtataat
     2641 gttccttcta gaacaggcat gtcgattcca atccatgtat tagtaaattt agcagaacat
     2701 cctaatatca ttggtttgaa agaagcctca ggagatatgg cttatgtgat ggatgccgct
     2761 cggttgattg gagaagaatt cttcctttac agtggaaatg atgatttgat cctaccagta
     2821 atgagtgtag gaggaagcgg tgtaataagt gtatgggcga atatccaacc taaaatagtc
     2881 catgaattag taaaagatac gcaggacgga agatggcagc aagcaaaaga aaagcaatta
     2941 aatgcacttg aactgatcca tgccttgttc agtgagacca atcctattcc tgtcaaagca
     3001 gccatgtcat tattagactt accttccgga cctctacgcc tgcctttagt ttctctttca
     3061 aaagagaaaa agaaacaatt agctcagctt ttgttaaaag aaaggacggt tgaaaaatga
     3121 aaatcggact gattggcagt ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa
     3181 gaggagatac ggtccttctt ttacggacgg cacctaaaga agcaactacg cttgctttta
     3241 cggaactgcc agaactgctt attgattttt ctcatccaga taatttagag aaaatccttt
     3301 cgtatagctt atcatatcaa cttccgttag tcattggcac gacaggttat acacaatcac
     3361 agttccaaga aataaaagaa catgccaaac atgtacctgt gatgtatagt gctaattttt
     3421 cgttcggcat catggtgatg aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct
     3481 ggcagatcga actgattgaa aaacatcata gtcgtaaaaa agatgcacca tctggtactg
     3541 caaatatgct gcttcaaaca atccaagaag tgcaaaagct gcagcccgta tacaactggc
     3601 aagggaaaaa aagagaagaa aaccaaattg gtgtacacag tattcgtgca ggttctttac
     3661 ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat atttacgatc aaacatgaat
     3721 ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc agcggactgg ttgaaagatc
     3781 agtcaccagg ttattacaaa ttagaagatt tattaatgga caaaggggtg taaaaaacgt
     3841 ggaagctcaa gaaatcatcc gctacattca aacggcatct aaaaagacgc ctgtgaaagt
     3901 ctatctgaat gtaaccgagc caatcgaatt taaagaaagt aaagtttttg gtgaaggaag
     3961 cagcttcgtt gtttttggtg actatgagac gattgctccg gttttagaag ctgaatcaga
     4021 aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc ctttgctaga
     4081 tattaaaaaa attaatgctc gtatcgaacc aggtgctatt atcagagatc aagtgtcaat
     4141 cggcaacaat gccgtgatca tgatgggagc gattatcaat attggtgcag taatcggtga
     4201 aaacacgatg attgatatgg gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg
     4261 ccatattggt gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt
     4321 tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg attgttgaag gtgtccatat
     4381 cggaaaagat gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg cagcagaaac
     4441 tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa
     4501 tacagcattg attgctgcgt tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat
     4561 agatacctgt attgtggggg caaccggatt agtcggccaa acgatgctca aagtgttaga
     4621 agaatatgat tttccagtag gacgattgaa attattagct tcaaaacgtt cagccggtaa
     4681 agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa cttcatttga
     4741 aggatatgat ttagcgctat tttctgcagg tgcttctgta tccaaagagt ttgcgccaat
     4801 cgcacgaagt cagggactat ttgtcataga taattcctca gcatggcggg aagattcgga
     4861 tattccatta atcgttccag aggtcaatct ggaagactat acattgaatc ggctgattgc
     4921 aaatccaaat tgttcaacca ttcaagcggt acttccgctt aaagctttac aagaagcctt
     4981 tggtatccag cgagtcaatt attcgaccta tcaagcagtt tctggttccg ggcaaaaagg
     5041 cattgaagat cttcatcgta cgcaaaatga cgaggaacca gttttgtatc cttataatat
     5101 tagccaaacg gtgattccgg aaatcgatca gcctttggaa aatgggtata caaaagaaga
     5161 acagaaaatg ataaatgaga cccgtaagat tctgcatcag cctgctttgc cagtatcagc
     5221 aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag ttcaactgca
     5281 aaaagagttt acgacggaag aaattagaga gtgtctcagc cagtttccag ggattcaatt
     5341 agaagatgat ctagcagcac ataaatatcc gacgagcatt cttgctagag gaacagatat
     5401 ggtttatgtt gggaggatca gaaaagacac ttctttagag aacggattgt tgttctatac
     5461 gactgccgat aacatacgca aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt
     5521 aatgcagaaa atagcggagg tggcaagatg agtaatacgc tgaaagtagc gaaattcggc
     5581 ggaacatcga tgtctcatga agagcaattc aaaaaagtcc gtacaattgt tctttcagat
     5641 cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa
     5701 gtaacagact tactttattt gatttatgct taccttcagc atcatgttga ttggacaccc
     5761 ctttggaaaa ggatttgtga tcgtttcatt caagtaagag atgttcttgc cctgcagtta
     5821 cctattgaag atgaactagc acgcatacag aaacaactgg aagacgaaaa gataactgaa
     5881 gattatttag taagtagagg tgaatatctg actgctcaac tgatggctga gtatcttggt
     5941 taccagttta ttgacgcaaa agacctgatt ttctttgact atgatgggaa aatcgatcag
     6001 gaaaaaacag cacaggaaat cacaaaagca tttaaaagat atccgaaaat ggttgttcca
     6061 gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac
     6121 attaccggcg cgatactggc gaagatgttg caagcggaaa gttatgagaa ttggacggat
     6181 gtttcaggaa tcatgatggc tgatccgcga atcatcgatc atcctaaaat catcgaggaa
     6241 atctctttta gagaactgag agagatggct tatatggggg ctaacgttct tcatgaagca
     6301 gctgttttcc cagttcaaga ggctgatatc cccatccaaa taaaaaatac caatgatcct
     6361 ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg actgacagga
     6421 atagctggac gcaaggattt tctttccatc actttattca aacgtcatat gtccgatgaa
     6481 atcgggttta tctggaaagc aatgagcatt tttgctaaac atgagatatc aatcgaacat
     6541 attccttcag ggatcgacaa tatcggagtg atcgtctcag cagaagcgat tgccgataag
     6601 ttatttctca tcacgaaaga attaaaagaa acacttggcg tggaagaaat cgtcgtgatc
     6661 gaagacttag ctttgatctc tgtcgttggg ggtccgcata aagaattgat tggattatct
     6721 gggaaagtgt tatcgatatt gaacaaatta gaaattcgca catcgatctt atcacaagga
     6781 gcacaggagt tgaatctgat catcggtgta ccgaataatc aatatgaaac agtagtgaag
     6841 ggtatctatg aagggatggt gaatacggat gctgaaagaa tatcgaatgg cactccatca
     6901 aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa
     6961 aaacagcgag gccgttatcc atgaaatcgg cgaaacagga ctactccttt attttgataa
     7021 tcagcaagaa gaaacgattg cttttcgaac agatatcgat gcactgccag tcaaagaaga
     7081 aacgaatctc tcttttgctt ctaagcatga aggttatatg catgcatgtg gtcatgacgg
     7141 acatatggcg atgcttttag ggttgactga ctttataact aaacacattg aggatcttat
     7201 ttataatgta gtactgatct atcagccatc agaagaaatt tccggtggcg cacaaagcgt
     7261 tattcagtca ggattgttgg ataaatatga agtacaagca atatttggct ttcatctatg
     7321 gcctggacta gctaagggag aagttttctc tcgaccagga cctttgatgg cacaaagcag
     7381 tgaaacagac attatcatta aaggccagtc tgcacatatt gcttctagtg aaaaaggaat
     7441 cgatagtctg gaggcagctg tacgattttt gaagaatgtt tatgattttg accggcaatt
     7501 accggcagag tggaatcatc taatgaaatt tggccaaatc caaggtggaa cgatccgaaa
     7561 tgttttagct ggagaagtta ttgtatccgg aagtatccgt tcttacagtt cagatatgca
     7621 aaattatttg aagaggggat tagaccattt agcagaacag tttcactcta cagctactat
     7681 ttcttttcgc tataatgacg gctatccagc tgtaatcaac catagaggct tatatgcagc
     7741 actgttagat tcaaacaaac tacatgaatt agatgctcca gttcttcaag ccgaagattt
     7801 tggcatatat acgcaacaat atccatgtgt atttttcttt ttaggtgtcg gtgatacccc
     7861 tgctttacat gatccgacgt tcgattttga tatggatgtt ttagaaaatg gtgtagaatg
     7921 gtacaaaacg attcttttta cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga
     7981 ggagcgagta aataatgatg aatttatcca atcgatttag tcctaggctt tctagaatag
     8041 aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc gataccagat gtgatcaaac
     8101 tcaccctggg agaaccggat tttccaactc ctgaacatgt caagcaagca ggaatagcag
     8161 cgattgaaga agacttttct cactataccg gaatgagagg attagaggaa ctgcgggaag
     8221 cggcttgtat atttcagcaa cagcgttatg gattaactta tgatccgcaa acggaagttt
     8281 tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt ttggaagaag
     8341 gagacaaggt tcttattcca gcgcctgctt attcaggata tcagccatta gtggaactag
     8401 ctggtgctga attgatcccg atagatactt ctgacacagg gtttgtctgt cagccggaac
     8461 aatttgaacg agcatttgaa caatatggat cagctgtcaa agcagtcatt ttaaactatc
     8521 ctaacaatcc tactgggacg acattgtctg caaaacaatt gaaagcaatc gctgaggttt
     8581 taaaaaaata tccggtattt gttataagtg atgaagtata tgctgaattg acttatagtg
     8641 gaacgcatat gtccatagct acttatcttc cagaacaaac aatcgtgata agcgggttat
     8701 ccaagtcaca tgcgatgact ggttggcgag tgggctttat ttttgctcaa aaagccctta
     8761 ttgacgaact aattaaagtc catcaatatc tggtgactgc cgctacaacg atgtcgcaaa
     8821 aagctgcggc tgaagcgcta ataaatgggg tgaaagatag cgagaatatg aaagagcaat
     8881 atcaaacacg aagagactat ctggtccagc aattggcgcc tttaggattt gaagtgaccc
     8941 agccaaacgg tgcattttac ttgttttgca aactcccagc tgccattcaa actgactctt
     9001 ggcagttttg tgtagaatta gcggaacagg caaaagttgc gtgtatccca ggaattgcat
     9061 ttggcccaga aggagaaggt tatatccgga tttcttatgc tagcagcatg gaaaaattac
     9121 atgaggcctg caaacggatc aaagactttt taacagaaca gtgattcata gattttatca
     9181 aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag acgattgttt tttaaattta
     9241 gttcaaatga acgcgtttct tttgattcat aactaatgta atgttaagtt taaattgttt
     9301 agaatgattt taaaagaata ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca
     9361 ttgtaggtgc atctcatgga gggcatcaat cgatcttgga actattgaca cgatacggtg
     9421 aaaatgtaga tatcacgcta tttgaagcag gagactatat ttccttcatg tcatgtggta
     9481 tggagctgta tttagaagac aaggtaacag atgtaaatga tgtacgtaat ttcagaccag
     9541 aaaactttcc gcaatccaat gttcatattt taaataatca cgaagtaaaa acgatcgatg
     9601 ccgataaaaa aacagtaacg gttactagcg catcagataa cgaaacaaaa gaatatcctt
     9661 atgacaaatt gattttgagt tctggtgtca aaccaaacaa tctgccagta ccaggagcag
     9721 atctagaaaa tgtctacttg atgcgtggat acaattgggc aaccaaaatc aaagaaaaat
     9781 taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag
     9841 cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt gcttgatgtg atcgatagac
     9901 ctttaggcac ttatttagat gctgagatga cagatatttt agaacaacac ctaaatgaaa
     9961 acggtattga agttttgaca aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg
    10021 aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct agtcattcaa gctgccggtg
    10081 tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc
    10141 aaacaaatga atacttgcaa acgaatcttc cagatgtgta tgcagtagga gatgctacac
    10201 ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta
    10261 gagaagcacg ttatgtagta aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg
    10321 gagtcgtagg ttcctctgca ttaagtgtct ttgactatca ttttgcagca agcggattga
    10381 atagctttac agcagcaaaa gcaggagtag atgtacgagt gtcttattat gaagatacgt
    10441 tacgaccaaa atatgtacca gaagaacaag gaaatccaaa agtgtccatc caattattct
    10501 cggatccaat gacccatcaa ttgctaggag gagcagtatt atctacctat gatgttacag
    10561 gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact gactttagaa gaactagcag
    10621 aagctgactt cttcttccag ccaggctttg accgacaatg gagcatgttg aatctagccg
    10681 ctcaacaagc gttaggagaa gaaccatttg tagaataaga aaacgaaata tttgaaaaga
    10741 ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagtaaaa acagcttctt
    10801 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattt
    10861 tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc aaaagttaga ccaaaaaatc
    10921 taacttttgg aggtcggtat tttttatggt taaatatact tttgaactta aattaaagat
    10981 cgttcatgat tatttggatg gcaaaggcga ttggaccgta aaaatccaga tcaagagatt
    11041 gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt atggctgttt acggatgacc
    11101 aatgaactaa gaaaccgcgg tttctctatc aataaaaaga aagttcagcg cttgatcaag
    11161 aagctaggaa taaaagtaac ggcatacacg cgtaaatcac gtcgctacaa ttcttatcga
    11221 ggaaaagt
//
LOCUS       DAYYBG010000087        11197 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000087 DAYYBG010000000
VERSION     DAYYBG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.088"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACHAUN_002240"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACHAUN_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8460914.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACHAUN_002241"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACHAUN_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8460915.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACHAUN_002242"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACHAUN_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8460916.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACHAUN_002243"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACHAUN_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8460917.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACHAUN_002244"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACHAUN_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8460918.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACHAUN_002245"
     CDS             complement(6343..7062)
                     /locus_tag="ACHAUN_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8460919.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACHAUN_002246"
     CDS             complement(7059..8372)
                     /locus_tag="ACHAUN_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8460920.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACHAUN_002247"
     CDS             complement(8453..8773)
                     /locus_tag="ACHAUN_002247"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8460921.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACHAUN_002248"
     CDS             complement(8881..9168)
                     /locus_tag="ACHAUN_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460922.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACHAUN_002249"
     CDS             9295..9939
                     /locus_tag="ACHAUN_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8460923.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACHAUN_002250"
     CDS             complement(9998..10705)
                     /locus_tag="ACHAUN_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8460924.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACHAUN_002251"
     CDS             complement(10925..11095)
                     /locus_tag="ACHAUN_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460925.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1956 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DAYYBG010000088        11184 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000088 DAYYBG010000000
VERSION     DAYYBG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.089"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHAUN_002252"
     CDS             6..305
                     /locus_tag="ACHAUN_002252"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8460926.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHAUN_002253"
     CDS             378..1739
                     /locus_tag="ACHAUN_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8460927.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHAUN_002254"
     CDS             1761..2021
                     /locus_tag="ACHAUN_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460928.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHAUN_002255"
     CDS             2035..2787
                     /locus_tag="ACHAUN_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8460929.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHAUN_002256"
     CDS             complement(2881..3486)
                     /locus_tag="ACHAUN_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8460930.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHAUN_002257"
     CDS             complement(3539..4513)
                     /locus_tag="ACHAUN_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8460931.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHAUN_002258"
     CDS             complement(4891..5610)
                     /locus_tag="ACHAUN_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8460932.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAUN_002259"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAUN_002259"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8460933.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHAUN_002260"
     CDS             7161..8558
                     /locus_tag="ACHAUN_002260"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8460934.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHAUN_002261"
     CDS             8639..9079
                     /locus_tag="ACHAUN_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8460935.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHAUN_002262"
     CDS             9227..9553
                     /locus_tag="ACHAUN_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8460936.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHAUN_002263"
     CDS             9554..10303
                     /locus_tag="ACHAUN_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8460937.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHAUN_002264"
     CDS             complement(10383..10838)
                     /locus_tag="ACHAUN_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8460938.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3550 a         1958 c         2296 g         3380 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgtt
//
LOCUS       DAYYBG010000089        10751 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000089 DAYYBG010000000
VERSION     DAYYBG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.090"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(294..1142)
                     /locus_tag="ACHAUN_002265"
     CDS             complement(294..1142)
                     /locus_tag="ACHAUN_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8460939.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1306..2208
                     /gene="gnd"
                     /locus_tag="ACHAUN_002266"
     CDS             1306..2208
                     /gene="gnd"
                     /locus_tag="ACHAUN_002266"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8460940.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2210..3211
                     /locus_tag="ACHAUN_002267"
     CDS             2210..3211
                     /locus_tag="ACHAUN_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8460941.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3208..3492
                     /locus_tag="ACHAUN_002268"
     CDS             3208..3492
                     /locus_tag="ACHAUN_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8460942.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3525..4322
                     /locus_tag="ACHAUN_002269"
     CDS             3525..4322
                     /locus_tag="ACHAUN_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8460943.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4336..5169
                     /locus_tag="ACHAUN_002270"
     CDS             4336..5169
                     /locus_tag="ACHAUN_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8460944.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5187..6044
                     /locus_tag="ACHAUN_002271"
     CDS             5187..6044
                     /locus_tag="ACHAUN_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8460945.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6066..6575
                     /locus_tag="ACHAUN_002272"
     CDS             6066..6575
                     /locus_tag="ACHAUN_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8460946.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6612..7013
                     /locus_tag="ACHAUN_002273"
     CDS             6612..7013
                     /locus_tag="ACHAUN_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460947.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7125..7478
                     /locus_tag="ACHAUN_002274"
     CDS             7125..7478
                     /locus_tag="ACHAUN_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460948.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7512..7955
                     /locus_tag="ACHAUN_002275"
     CDS             7512..7955
                     /locus_tag="ACHAUN_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8460949.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7972..9057
                     /gene="uxuA"
                     /locus_tag="ACHAUN_002276"
     CDS             7972..9057
                     /gene="uxuA"
                     /locus_tag="ACHAUN_002276"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8460950.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9050..9763
                     /locus_tag="ACHAUN_002277"
     CDS             9050..9763
                     /locus_tag="ACHAUN_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8460951.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9970..10119)
                     /locus_tag="ACHAUN_002278"
     CDS             complement(9970..10119)
                     /locus_tag="ACHAUN_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460952.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10515..10715
                     /locus_tag="ACHAUN_002279"
     CDS             10515..10715
                     /locus_tag="ACHAUN_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8460953.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3606 a         1696 c         2318 g         3131 t
ORIGIN      
        1 aggaaatact taattcacca aaattttttt taatttttta gaaaataaga aacaaaaata
       61 ataaaaaaaa gaaaagataa attttttact actgtatact aactttgtta tcgcatctaa
      121 caagtaactc gaataatacc aatacaacct tctccaaaga tgcgatgaca tacgttctaa
      181 cccaccgact attgatgttc tgtaccaccc ttgcagaacc aaaaaagcca ctaccaaata
      241 tttggtagtg gctttttttg ttttatggat ttttcgcttt ttctgttatg gtgctattct
      301 ttgatttcag aaatagtatg tcgaacttta gcgatagaac gacgtgattc tttttcgttg
      361 taaaacatca aaatcacata tagagagtcc aaaatgctta gttgagaaat acgggaagct
      421 aaagcttcag atcggaattc tgtttcttca gaaatagaaa taaaaacgac atcgccaagt
      481 ttagcaaggg aagaattagc ttggctagta ataacgattg tttttgctcc agtttttctt
      541 acggtttcag caatatggat tgtttctttt gattgccctg tgtgagaaat caaaacagca
      601 cagtcttttt ccgtcaataa agaagcttcc atcaattgaa tatggtaatc cgtgctgtga
      661 gaagttgcga taggggaacg caaaaattta tgataagcgt cagttgctac aatctctgaa
      721 ccgccaacac cgaaaaaata gacacgttta gattcattga taatagtagc cgcttttttt
      781 agatcctttt gttgtaacaa cttttttgtg tcatttaaag ttgaaatact agactcgaac
      841 accttttgcg ccatagtgag ctcagtatct gttttctgaa tattttcgtg gatagaaata
      901 gcagatagat cattttcttg gatcaacatg gccattttaa aatctttaaa tccattataa
      961 ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa tgcccagttc acttgctaaa
     1021 ttgctaatag gactatgact tactttgctt ggattttcta aaatataatc ggcgatagcc
     1081 tgttcttttg ggctgaagtc gttgtacatt gttttgattt gtagtcttat ggagacgctc
     1141 atgagaattc ctcattttca taataagatt atcatttttt ccgttctcat taatgttaaa
     1201 gatacaagga gagtaggaaa aaatcaaaca aaaaatattt ttgtaaaatc tatttacaaa
     1261 aaaatttttt tgtataatga gggtataaga aatggaggcg ctatcatgga aattggtgta
     1321 attggtttgg gaaaaatggg attgaattta gctttaaact taaaagagca ccatcacaaa
     1381 gtacaaggac tcgacatttc aagtacagct gttagtcagg caagagaaaa tgacattcct
     1441 gcttatcaag agatctctga atttctagct acttttgagc agaaaaaaat catctggtta
     1501 atgcttcctg ctggagagat aaccgaaaac atgttaagaa aactttttcc aaaactcgga
     1561 ccaggagaca ttattattga aggcggaaac tcttattata aagattcaat ccgtcgagca
     1621 gaagagtttc agaaaaatga aatcggttac ttggattgtg ggacttctgg aggaatcgaa
     1681 ggagcacgac atcaagcctg cttaatgatt ggaggagata aagaaacttt tctatccgtg
     1741 gaacaattat tcaaagatgt agcaattgaa agaggatatc tgtatgctgg tgcatccgga
     1801 agcggacact tcttgaaaat gatccataat ggaatcgaat atggaatgat gcaagcaatc
     1861 ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt ttgacttgga acaagtcgca
     1921 cgtgtttgga atcatggttc ggtgattcgc ggctggctga tggaaatcat ggaagatcag
     1981 ttaaacgaac atcccaattt aagtaattat cggggaatcg tttctgcatc aggtgaagca
     2041 aaatggacgg ttgaaacagc gctagaaatg gctgttccag ttccagtaat tgcaactagt
     2101 ttgttcatga ggaatctttc acaggaagaa gatagttttt cagcaaaagt agtttcagca
     2161 ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg gtgagtaaaa tgccagatta
     2221 tcatatagca atcgttaatt ccagcagttt cggaagagtc tttccacacc acttggaaag
     2281 attaaagtca gttggagacg ttcatttctt tactgttgat caagagattc caggaaaaga
     2341 actagcggag ttacttaaag gattcaatat cattattgca agtgttacgc cattttttcg
     2401 acgagatttt tttgaaaaca aagatgaact gctattgatt tcccgacatg gtattggtta
     2461 caacaatatt gatttaaaag cagcaaaaga acaccagaca atcgtatcta tcatacctgc
     2521 tttagttgaa cgaaatgctg tagcagaaaa taatataact aatttgttgt ccatgcttcg
     2581 acgcacgaca caagcacaag caagagtcaa ggacgatcga tgggaagatc gggcggagtt
     2641 cgtcggtagg acgctgttta acaaaacagt tggtgtgatt ggtgcaggaa ataccggaag
     2701 cggagtaatc gaaattttgc gtaacggttt tcgctgtgat gttctagctt atgatcctta
     2761 caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa gtagaacttc aagaattact
     2821 ggaacaatca gatatcattt gtttatgtgc gaatttgaca gaagagaact atcacatgat
     2881 ttctgataaa gaagtcgatc agatgaaaga aaatgtttat ctatctaata gtgcaagagg
     2941 cgctctagtt gatgaagaag cgatgattaa tggtctgaaa agcggaaaga tcgcaggata
     3001 tgccacagat gttttagaag aagaaccggg acgtcaagat catccgtatt tagcgtttga
     3061 acaagtagtg attacgccac atatatcggc ctacacaagg gaatgtttag aagcaatggg
     3121 tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac cttcttcctg aacgttctgt
     3181 ccagacaaag agcaactata taaaaggatg atgaagaaaa tggtgaacgg tttaaaagtg
     3241 aaaacaggcc ctcaatttta tttatatgaa gaaggtggta tttcaaaagt ttctgatctc
     3301 ttgaaatcat acggcgcgaa aagagtactg gtcacccacg gaacggtatc atgggaaaag
     3361 gcgctaccta aattagtatt tttgaatgac gaaacgattc aattttttta tcatcgttat
     3421 tctggagaat gtagttatgc tgaagccaga cgaatagcta caatcatcaa aaaaatgaaa
     3481 tcgatttcgt agttggcgtt ggcggaggaa aactgaccga tctagtgggt tatagtgcca
     3541 atcttgcaag aatcgatttc ggagtcatcc ctactttagc tagtaattgt gctccatgga
     3601 cacctcttgc tgtcatgtat aaggagaatg gacaagcaga agggaaaaca gaacactttt
     3661 acagacaagc tgcttttttt ataacagatc cccaattagt gattgattcc cctgttccat
     3721 atttcattgc cgggttagca gatacactag caaaatggta tgaatctgag ctcatcttag
     3781 aacaagattt tttgcaagat gaatcatttt tgaagctagc acaagatact gcgaagatct
     3841 gcaaagaaga gattttgaat cattctgcta aagcaatcca agatatggca caaagaaaac
     3901 tgacatcgga gttcaaacag ctttcagaga ttatctttgc tgttgctggt ttagttggag
     3961 gattaggaga taaatacgca agaaatgtgg cagcacatgc tatgcatgac gctatcagta
     4021 aatatattcc ggaaagccat cgttttctac atggagaaaa agtagcgtat gggattttct
     4081 atcaactggc actagaaggt cgctgggaaa aaatagacga gttatatcct ttgtatcggg
     4141 aattagaact gccggtttct ctagaacaaa tgggattatt tccgaaagat aaacagataa
     4201 tagataaaat ggctgaattt attgattcga aagagaaagt gcatttgatt cctatagagg
     4261 taacacagaa atcactgata cagtcaatga tggaactgga aggttatatc agaaagatct
     4321 gaaggaggag gacacatgga aacattaagt gtagtacaaa gtttgttgat tgctttatgg
     4381 gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca cgttgacctt acgtttttct
     4441 cctttgatga ctggattagt cgtcgggttg atcatggggg aagttgcaca agcgatgatt
     4501 gttacagccg ctttgcaaat gatttatatg ggggtttttt ctccaggagg atcaatgcca
     4561 gctgaaccat ctattgcagc agctatcgca gtaccagtcg cattattagg ggatctgtcg
     4621 ccagaagcag cgattgccgt tgcggtgcca gtcggattgc tcggaagtta cttgtatcag
     4681 tttcgttttt tcatcaatac ttttttaggt aaatatacag acaaagctgt agaagatttg
     4741 aatacaaaag ctatggcccg atcaattatt ctttatccaa cggttgcctc atttttatta
     4801 ttcgttccgc ttgttttcat tgctttatat ttaggggcgc cattgatcgc tgatgtaatc
     4861 aaagctttag agggaacagt cgttatccac attcttgaag tagtaggtgg agggttagcg
     4921 gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa aagattatct ggtctttttc
     4981 ttccttgctt attttatgag tgttgttttg aaatctttag atattacgat ggttacttat
     5041 gccatttttg ggattattat cgctttgatc tttgtccagt cgcaaaaagg gaaacaagta
     5101 cagcctgcca caagcaataa caattcagca tcttttgatg acgaagatga ctatgatgac
     5161 ggattttaat ttaggaggaa tgggaaatgg ctgaaataat ggataatcaa acaggtgctc
     5221 ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct gagatggcat tttgcaaacg
     5281 aaattcctca ctcttttgaa cgttacttgg cgccatcact actatacgga atgatgccaa
     5341 tcttgaaaaa actttataaa gatgaagact ctttaaaagc agcatataaa cggcagttac
     5401 tgttcttcaa cactcaatta agctggggtg gcggtgtgat tactggccta atgtcttcta
     5461 tggaacagca aagagcagaa gaagagtatg aagaaaaaga gatcttgatg caggatgact
     5521 tgatgtataa cacgaaagcg ggattgatgg gggcactagc aggaatcgga gatgcaatcg
     5581 attcaggaac cgtccaatat atttttatcg caatcgcggt accttgggca caacagggaa
     5641 gtcctttagg cgcacttttt ccttttatct gctttgggtt gtatcaggtt ttactagggg
     5701 tatttttcgc aagacaagct ttcactatgg gccgtaatgc tacaggccta aagcaaaaca
     5761 ctggtgttca gacagcgatt gaaatgctat cagttttagg gttgtttatg atgggggtgc
     5821 ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt tgcgatatcc ggacgtgagt
     5881 ttgttatcca ggaaattctt gatcaaatcg tgccagggat attaccttta gctgtcgtaa
     5941 tgggtgtgta cttgttctat acgaaaaaag gattgaaagt cacacaagca ttattatggc
     6001 tgactggaat attaattgtg ctagcaggaa tcggtatttt ataaaaagaa gggaacgtga
     6061 ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg tttgatccat gggcaagtaa
     6121 tggtaacgtt atctcaaaaa agtggagtga attccatttt tgttgtagac gaagttgtag
     6181 caaaagataa atttatgaga gatctttaca aaaatgcagg aagccgtaca ggacaaaaaa
     6241 caattgtcat tacaccagag aaagctaaat tctattggga tgaatatcaa tttaaagaat
     6301 acaattgtat tttgatcgca aaaacagtcc aaacgattta cgacttagta aaacacggaa
     6361 tccccatgga agaattgaat attggaggga ttgctcaaaa aaatcctgat acagatcttt
     6421 tagtgacgaa atctgtttat ttaaataaat cagatgctga aaaattgaaa gaaatgaatg
     6481 aacaatatgg cgtaaagaat atttattttc aagccactcc atctgcacct aaaacaggat
     6541 taaaagatgt attgaacaag tttgatttgt cataaacaaa tgcttagaaa aaagataaag
     6601 agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg ggaatttcgt tatcgctata
     6661 tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt tctttcagca ttagtatcag
     6721 atatttacag tatgagaaca gatgtgacag tcattgctta cgatacgcta gcctaccgat
     6781 caaaaaatat ttatgttgga gatatcgaaa aagcagagaa agttatctgt acttattacg
     6841 atacacccgt tcatgcatta ggaagttatt tcatgtttga ttggaaagac caaaggaaaa
     6901 aaacaatcta ctctatatta ttgtccttca ttttgttgtt cagtttaggt tggtggggaa
     6961 tgatgatata taacaaaaat cctcatcacg tattcgatct gctatccgta taaacaggta
     7021 tcactgtact agcatttggg agctactttt tcttattggg aaaagcagct cgcggatggc
     7081 ctagcagaca aacttttatt cgaaatactt catccatatt aacgatgcta gaaatgatcc
     7141 gtacgattga tgatccgagt gtagcgtatg cgtttgttga tgaaggctgt tatggagaaa
     7201 agggcttaga cagcgtgaga tcgggtatga aaaaagaggc tattcttttt tatctagact
     7261 ctgttggggc agatacaccg ctacagtttt cgggaaatta tttttcaaat aaagaacagt
     7321 ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa ttatattttt agtgcaagaa
     7381 aaaagcaagc gcagttcttc tatttgacca aaacagattt acgagggaaa acatttaatt
     7441 ggcaaaatgc caatcagatt attgctcttt ttcgataagt aaaaagtgca aaacaaggag
     7501 tgagaagact aatgttggaa atcatgatcg ttacacatgg taagttgagc gaaggactga
     7561 aagatgcggc ggaagtaatt gtcgggacat cgagccatat tgaaacaatg tgcttagagt
     7621 ctggggagga catccaaaca ttcggagaga aggtcaaaga aaaattaact atgatcaaca
     7681 cagcagaacc catgctagta tttgttgatc taattagcgc aagtccgtac aatcaagcgc
     7741 tcttggcagt caatgaactg actgaagaaa agaaagcagc cacttatgtc atagggggcg
     7801 tgaatctgcc aatgttgtta gaagcaatca atcatcagat gcttcacaca ccgatagaag
     7861 aagcggtgga acaaattgtc aaacaaggaa aagataattt aactgtttgg catgcttcag
     7921 cggaagaaaa aaaattagat gaggacgatt tttagaagga aggctgaaaa gatgaagtgg
     7981 ggatttagat ggtatggtga aaaaggggat tcgatcccat taaaatatgt tcgccaaata
     8041 ccgggaataa atgggataat tggaactttg ctgactaaaa tgcctggaga tgtttgggag
     8101 atttcagaaa ttgaggagtt gaaagcatct gttgagagcc aaggactgga actgcttgga
     8161 attgaaagtg tagcgatcca cgatgcaatc aaggcaggga cagatgaacg agattactat
     8221 attgataact atatacgaac aatcaaaaac ttgtctcgat gtggggttcg attgatttgt
     8281 tatagcttca aaccaatttt tggatgggcg aaaactgatt tgtttaatga aaacaacgat
     8341 ggaagctatt ctcttttata cgatcagaaa atcgtcgatc agatggagcc aggagaaatg
     8401 tatcaattga tacacagcca gtcaaaggga ttcaaattgc cgggttggga agaagaacgg
     8461 ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa tgaccgaaga agaattattt
     8521 gaaaatctga cttatttttt agaaaaagtg attccagtgt gcgaaaaagc agatgtaaaa
     8581 atggctatcc atccagatga tccgccatgg gaactttttg gttttcctag agtgacgaaa
     8641 aatcttggcg acttgaagaa aattctaaat gcagtagatt ctccatataa tggactaacg
     8701 ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt tagttgaaat catccatgag
     8761 gtaggttcgc gcatccattt tgtccacttc agaaacgtgg agtttttagg agaaagaaag
     8821 ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg atatgtatca tattatgaaa
     8881 gcgttggtgc aagaagggtt cgacggtcct atacgccctg atcatggtag aaccgtgtgg
     8941 ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg caatgggact cacttacatg
     9001 caaggtctat atgaggcgat aagtaaggaa aaaccactgg gagagcatta tgaataaaat
     9061 caagctcttc atctttgata tggatggcct tttattagaa actggacgtt tagcttatcg
     9121 tgcttatgta aaatcagcac aaaagtatga ttatgaaatg cgtaaggaag tctattattt
     9181 actaactggc caaacagaaa tggcgataag agaacaaatg ggcattttgt acggagaaga
     9241 tgttccatat atcaaatggc gagaagcaat caatcaatat aaagaaaaaa tcgtgaaaga
     9301 agataagaga gtatatacga aaaaaggagc agaagaaatt ttatcttttg caaaagaaag
     9361 aggaatccat acaatcgtgg cttcgtctaa tacacgggta aaagtagaaa tgtatttaaa
     9421 aatggaaaat ctatatgcct tgtttgatca aatcatttca ggggatgatg taaaaaaagg
     9481 aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa atgaatattc ctccaagtga
     9541 agcacttgta tttgaagact cgattgcagg gatcgaagca gcaaggcgag cgggcatttt
     9601 gtcttttctc atcaaagatc atttgatagg gttgcccgat cataatggga agcataagtt
     9661 gaaagtagat gtaagtttat atgaatccaa taaagcagac tatgaatttt atgacctgca
     9721 tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc taataaagag gactattttc
     9781 tagtcctctt tatttattgt cctgcacgac tgagggaaca gatgaaaaag aagtaatttt
     9841 ttattttgaa gtatctacct tatttgtagt gaacaattta gaacaaacac aaaacgcatc
     9901 tactgttatc taccagtaga tgcgtttaaa gagaagaacg agaatcgtcc atatgagggt
     9961 ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg taacacctgt
    10021 cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa
    10081 ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc tttctttaaa
    10141 tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag tataccacca
    10201 attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata aaagcaaata
    10261 aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg attcgaagac
    10321 aatagaagac aatgaaattt atatttcatt gcattatcct tgtgttatta gaaaaagcat
    10381 gatatattaa atgagtaatc tattttgaaa cgtaatttga gcgatatatc tacatagata
    10441 aaactccttt tgcctatcaa atatttattg caacgaaaaa acgtacattg atacgtatca
    10501 ggaggaattt atttatgaat aacggtacag taaaatggtt caacgcagac aaaggttttg
    10561 gctttatcac tggtgaagat ggaaatgatg tatttgcaca tttttcagct atccaaggtg
    10621 acggcttcaa aactttagaa gaaggccaag cagtgtctta cgatgttgaa gatggtcaac
    10681 gtggccctca agcaacaaac attgttaaag cttaataaaa attgactttt aaccgtcttt
    10741 tcgaaagaaa a
//
LOCUS       DAYYBG010000090        10526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000090 DAYYBG010000000
VERSION     DAYYBG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.091"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..1656)
                     /locus_tag="ACHAUN_002280"
     CDS             complement(97..1656)
                     /locus_tag="ACHAUN_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8460954.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1811..4045
                     /gene="pcrA"
                     /locus_tag="ACHAUN_002281"
     CDS             1811..4045
                     /gene="pcrA"
                     /locus_tag="ACHAUN_002281"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8460955.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4062..6098
                     /gene="ligA"
                     /locus_tag="ACHAUN_002282"
     CDS             4062..6098
                     /gene="ligA"
                     /locus_tag="ACHAUN_002282"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8460956.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6204..6509
                     /gene="gatC"
                     /locus_tag="ACHAUN_002283"
     CDS             6204..6509
                     /gene="gatC"
                     /locus_tag="ACHAUN_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8460957.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6509..7981
                     /gene="gatA"
                     /locus_tag="ACHAUN_002284"
     CDS             6509..7981
                     /gene="gatA"
                     /locus_tag="ACHAUN_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8460958.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7978..9408
                     /gene="gatB"
                     /locus_tag="ACHAUN_002285"
     CDS             7978..9408
                     /gene="gatB"
                     /locus_tag="ACHAUN_002285"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8460959.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9418..10473
                     /locus_tag="ACHAUN_002286"
     CDS             9418..10473
                     /locus_tag="ACHAUN_002286"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8460960.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
BASE COUNT         3588 a         1973 c         2308 g         2657 t
ORIGIN      
        1 aggctgggtt ttcccagcct cttctttttt tatagaatca tatagaatca agtagtgtag
       61 atgaatagtt gttgtaaaat agtttttcca tgttcgttaa aatgattgct tcaaatttgt
      121 gatagaagat ttagcctcga tttcctgctt gattcttggc aagtttgctt catcagtcaa
      181 gataaccaaa atatctccta cattcatgat cgtatcaccg tgagtcaaga tttcctgatt
      241 tcctctgcgt atagagatta gcagcatatt tttaggccaa ttgaagtctc gtaccattaa
      301 tccgtctaaa gaactctctg ccgttactgg aaaatcaaaa gttgtccttg caccttttag
      361 tgaggcggtc tgagtatttc caagtagacg ttccaataat gattcgtaaa ttggattacc
      421 gccgagaagg tcattgacga tataagcaac aagagcagcc aatcctaaag gcatcagatg
      481 attaagagag ccaaccattt ctgtcactaa aataattgct gtcagcggtg cttttccaat
      541 cgctgtgaaa taaccagcca tggcaataac gacaaaatcc ttgatgtaga ttggatccat
      601 cccgaaatca accataatgt ttccataaaa gctaccgatt aaagccccca atgtcaatat
      661 cggaaggaaa atcccccctg gaagaccccc accgtaagaa atcatggaaa aaacgaatcg
      721 aagaacaaac aaaccgataa tcataatgat tggcaaggaa gcatgtccaa aagctaaaac
      781 gatttgattg cctccgccta gagtactagg ccataaataa ccaattggca aaatcagcaa
      841 gaaaggaacg aacccgtgaa aataagctgg taaaggcaat cggttgtaaa attttggcaa
      901 agccagcaag acattctgat aaataaaacc taagatgcct agaagtactc ctaagatcac
      961 taaggaacca taatatttca acggcaaaga aggaaggtca cctagatata aaacaggtct
     1021 caagccgaaa aagtaaagag agacgaagtt agcagtgatt gctgaagcaa atgatgtcag
     1081 ccaaacaagt ggagaaaagg agtgatggat ttcttctaaa acaaagagta atcctgcgat
     1141 aggagcattg aatgctgctg caagtccgct acttgcaccg ctagaaatta aaatcttttc
     1201 ttctgaagat ggactccgaa acaattgtcc agctccttgt ccaatggatg cgccaagctg
     1261 tatagaaggt ccttctctac ctagaaaaag gcccgcacca actgaaagta caccgccaat
     1321 gaactttttc cataaaacag agaaccagtt cattgataat tccccgcgaa gctgtcccac
     1381 tacttgtgga atcccgcttc ccttgatatc cggttcattt ttgaccagat aacccacgaa
     1441 aaatgctgca aggatgcagc aaatagctag gccaattaag taaatcggct gttcccgact
     1501 catttcatag atacttcgaa ctaaatcgct tagcttttcg atagataatc gaaacaggct
     1561 aacgacgatt cctgtcagca gtccaacaaa tattcctttt agaataaaat atatctgagt
     1621 cttatctaat ttcttgattt ttcctttttt ttccattcaa acgcctcgct ttcttttaat
     1681 ttagcgcgaa atcaacgaat tgacaaggaa aaatccataa aaagaaacaa atgattttga
     1741 actagctagt ttaatgaaag ccaagaaaat gctataatta acaagattga taaaagaaag
     1801 gatgttgtta atgtcatcca aagctgaatt attaaacggg atgaaccccc gtcagaaaga
     1861 agctgttctt cacactgacg gaccattact gctaatggca ggtgcaggaa gtggaaagac
     1921 gagagtgctg acacaccgca ttgcctattt gattgaggaa aaagaagtca atccttggaa
     1981 catcctagcg attactttta ccaataaggc tgcaaaagaa atgaaagaac gtgtcaatgc
     2041 gattctcgca tctggaggcg aagatgtctg ggtatcgact tttcactcga tgtgtgtgcg
     2101 tattttaaga agagatgttg attttatcgg gtataaccgg aactttacaa tcatcgattc
     2161 ttctgaacag ctgaccttga tgaaacggat actaaaagaa ctgaacattg atccgaaaaa
     2221 atatgatcct cgaagtatat taggaacaat ctctcaagca aaaaacagcc tgcagacccc
     2281 tcaggatttt gcaaaaatgc aaggcagtta ttacgaagag attgcggcaa aatgttatgc
     2341 tgcttaccaa aaagagcttc aatataatca atgtatggac tttgacgacc tgatcatgaa
     2401 tacgattcga ttatttgaag aacatcctga ttcactcacg tattatcaaa acaaattcca
     2461 ttatattcat gtagatgagt accaagatac aaatcacgca cagtatacgc tagtcaattt
     2521 attggcaggt agatttagga acctgtgcgt cgtgggagat gcagatcaaa gtatttacgg
     2581 ctggcgcggt gcagatatgc agaacatctt ggattttgaa aaagattatc cagatgctgc
     2641 agtaattttg ctagaacaaa actatcgctc cacgaaaaac atccttagcg cagcaaacca
     2701 agtcattgaa aacaatagca atcgaaaacc aaaaaacttg tggacagaaa acaaagaagg
     2761 aaacaagatc acttactacc gtgcagataa tgaacgagac gaaacccgtt ttatcgtaga
     2821 ccggatgcaa gaagagattc gaagcaatca tcggaattac ggcgactttg ccatcttgta
     2881 ccggacgaac gcacaatctc gtgtaatgga agaaactctg ttgaaagcaa atattcctta
     2941 taaaatggtt ggagggcaca agttctacga tcgtaaagaa atcaaagata ttttagcgta
     3001 tttgaatgtg ctggtaaatc ctcaagattc aatcagtttt gagcgtatcg ttaattcgcc
     3061 caaacgaggc atcggaccag gatctatcga aaaactacgc agttttgctt ctcttcatga
     3121 atggccactg cttgaagcag cgcaaaatgt ggatctggca aatatcggtg gaaaagcagg
     3181 acagcaatta ggtgcatttg gagagatgat ccaagaagtc actcagatga tcccatactt
     3241 gacagtgaca gaattgacga aagaggtttt ggatcgtagc ggatatctag aagacttaaa
     3301 aatccaaaat acattagaag cacaagcacg catcgaaaac ttggaggaat ttttaacggt
     3361 cacacaagaa ttcgacaaac aattcgaaca gcaaaatgaa gaagacgctg atgcaccaga
     3421 agaaaaacta acggtcttct taaatgatct ggcacttgtt tccgacattg acaatttaga
     3481 agaagatgct tcccaagtga cattgatgac tttgcatgca gccaaaggat tagaatttcc
     3541 agttgtattc ctgattggtc tagaagaggg ggtttttcct ttatctcgag cattgatgga
     3601 agaaagcgaa ctggaggaag aacgccgtct cgcatatgtg gggatcacgc gagcagaaga
     3661 agcgttgtat ttaacaaatg ctttctccag aacattatat ggacggacgc aatataaccg
     3721 accttctcgt tttgtagaag aaatcgatca ggagttactg gaaatcgaag gcatgcgtcc
     3781 tacgcctaaa aaaacgcctg tattcgctaa aaaaacagca tatagctata aacagccaga
     3841 gacagcggtt gttccctcta aaagtgctac tggaggggaa aataacagct ggaaacctgg
     3901 agataaagtc aaacacaaaa aatggggtct aggaacagtc gtccgagtaa gcgggacgtc
     3961 gaaggatcta gaactagatg tggctttccc aagtcaagga gtaaaacgtc ttttggctgc
     4021 ctttgcgcca attgaaaaag cttagtagtg gagggtaaat gatggaacat gaaatgacga
     4081 ttgacgaagc tgctaaacga gcagaagaac ttcgtacaag actgaaccaa tggtctcgcg
     4141 aatactacgt agaagataaa ccaacagtgg aagactatgt ttatgataaa gaatacgcag
     4201 aattagtagc aatcgaggaa caatatcctg atttgatcac gtctgattct cccactcaac
     4261 gagtaggcgg aaaagtatta gaaggttttg aaaaagtcac tcatgatatt ccgctttaca
     4321 gtttgaacga cgttttcagc aaagaagagc tgatagcttt tgatcaacgt gtacaaaaag
     4381 cagtgggacg tgtagttgac tattgctgtg aactaaaaat agatggactt tccgtttctt
     4441 tgcgttatga agatggaaat ttcgtgagag gtgcgactcg cggggatgga actgtaggag
     4501 aaaacatcac tgaaaaccta aaaactgtcc ggtcggtccc aatcaaatta aaagaaccaa
     4561 tgaacatcga agtaagaggc gaatgtttca tgccaaaacg gtcatttgtc caattgaacc
     4621 aagacagaga agcagaggga aaagatatct ttgcgaatcc tcggaacgcc gcagctggtt
     4681 ctttgcgtca gttagattct aaaatcacag cgaaaagaaa tctggacact tttttataca
     4741 cagtagctga tttcggtcct atgcaagcaa aaacccagta tgatgcgcta gaagagctag
     4801 aaaaaattgg attccacacg aatcgggaaa aacgtctatg tcattcgatt gatgaagtgt
     4861 ggtcatacat tgaagaatat catgacaaac gtgtggattt gccctatgaa atcgatggaa
     4921 tcgtcatcaa agtgaatgaa ttctcgctac aagatcaact tggttttact atcaaagcac
     4981 cgagatgggc agcagcttac aagtttccgc cagaagaagt tgaaacgcta atcgaaaaca
     5041 tcgaatggac agtcggtcga acaggggtag taacgccaac agctatcatg acaccagtcc
     5101 gagtagcagg aacgacagta agccgtgcca gcctgcataa cggtgattat attaagttga
     5161 aggatatccg cttgaaagat acagttctga tttataaagc gggagacatc atcccagaag
     5221 tttcgcaagt cgtgttagat aaacgaccta aagacagcga agaataccaa ttgcctacac
     5281 attgtccagt ttgtggaagt gaacttgtcc atttggatga agaagtagcc ttacggtgca
     5341 tcaatcctaa atgccctgct caaatgaaag aaggtttgaa ccactttgtt tcaagaaatg
     5401 cgatgaatat cgatgggttg gggccgcgcg tactagaaca gatgtatgat aaaaaactag
     5461 tagcagatgt tgctgatctt tacaaattga cagaagaaga gttattgacg ttggacaaaa
     5521 taaaagaaaa atcagcaaat aatattttga cagcaatcga caacagcaaa gataattcgg
     5581 tcgagcggtt gattttcggg ttaggaatcc gacatgtcgg agcgaaagca gcaaaaattt
     5641 tagcagaaca tttcggtgat ctagaaacat tgagtaaaag tgactatgag tcgattattg
     5701 cgcttgatac gatcggtgat atcattgccg acagtgtagt cacttatttc agtaacgaag
     5761 aagtccatga attgatgaat gaattaaaac aagcaggcgt caatttcgaa tacaaagggc
     5821 ttcgaaatgc ccaattgcaa gaagtcgagt ctccttttaa agaaaaaaca gtagtcctta
     5881 ctggtaaact aacacgattt actcgtgaag aagcaaaaga gacgatcgaa aatcttggcg
     5941 ggaaagtgac tggtagcgta tcgaaaaaaa cggatatcgt ggtagcagga gaagatgctg
     6001 gcagcaagct gaccaaagca caagaattag gaatcgaagt ttggacagaa gatcaaatgg
     6061 cagatgcatt ggctaaaagt cgctcagtag aagaatgaaa aatgagtttt ttcagacaaa
     6121 tttcaataaa caatttcaga cacggcactc ttgtgttaaa atagagagca aagtttttaa
     6181 ctcgtttaga aagaaggggt agtatggcga ttagtgaaga acaagtgaaa cacgttgcca
     6241 aattagcaaa acttgcgttc gcagatgaag aacttgcaag ttttaccgac caattaggta
     6301 aaatcatcga tatggttgaa atgctggaag aagtagatac ggaaggtgtt ccatttacgt
     6361 cgaatgtggt tgaaacagta aacgtgatgc gacaagatcg ggcgcaagct ggatggaacc
     6421 gcgatgaatt attgaaaaat gtgccggaaa cagaagacgg ctttatcaaa gtacccgcaa
     6481 tcatcgataa tggggaggct ggcgcataat gacagaatta cacaatcgtt ctctagaaga
     6541 actgcatcat atgcttgtat cgaaagaaat cactgtacaa gatttgacaa aaacaacatt
     6601 tgaacgaatc aaagaaacag agcctgaggt cgaggcattc attacattga acgaagaaaa
     6661 agcattggaa caggcaaaag ctttagatga aaaagggatt gatgaaagca acgtccttgc
     6721 tggtattcca attggtatca aggataatat cgtaacaaaa gatatcttga caaccgctgc
     6781 atctaagatc ctttacaatt ttgaaccaat ttatgatgca acggtcatgg ataaaatcta
     6841 tcaagcagat atgatccctg ttggtaagct aaacatggat gaatttgcta tgggtggaag
     6901 tacagaaact tcttatttca agaaaacaaa aaatgcttgg gacccaacaa aagtacctgg
     6961 tggctcttct ggtggttctg cagcagcagt tgcagctgga caaattccag tttctttagg
     7021 tagtgatact gggggaagta tccgccaacc agcagctttt aacggcatcg tagggatgaa
     7081 acctacttat ggccgtgtct cacgttttgg tttgattgcc tttgcttcat ctcttgacca
     7141 aatcggacca atgacccgta cggtgaaaga taatgcatta gcattgacag ctatcagcgg
     7201 ttatgacgaa aaagacggta cttcttcagg cgtttcagtt cctgattttg cagagggact
     7261 gacaggcgac atcaaaggaa tgaagattgc tttacctaaa gaatacttag gcgaaggtgt
     7321 ggcccctggc gtaaaagaag ctgtattgaa agcagcagaa acattccgtt cattaggtgc
     7381 tgttgtggaa gaagtcagct tgcctcattc aaaatacggt gtggctgttt attatatcat
     7441 tgcctcatca gaagcaagtt ccaatttgca acgattcgat ggtatccgct acggttatcg
     7501 ctctgaaaat gtgaaatcat tagatgatgt atatgtcaac tcacgttcag aaggattcgg
     7561 tgaagaagta aaacgccgta tcatgttagg tacattctcc ttaagcgcgg gttattatga
     7621 cgcttacttc aaaaaagcag gacaagtccg tacattgatc aaacgagatt ttgaaaaagt
     7681 ctttgcagat tatgacttga tcatcgggcc atcttcacca acagttgcat tcggaatcgg
     7741 tgagaacatt aacgatccaa tcacaatgta catgagtgac atcttaacga tccctgtcaa
     7801 cttggctgga cttccaggta tgtctgtacc ggcaggttta tcagaaggat taccagttgg
     7861 attgcagatc atcggtaatt attttgatga aaaaacgatg tatcaagcgg catatgcgtt
     7921 tgaacaagca acagaattcc ataaacaaca accagctatt ttaggaggga aagacgaatg
     7981 aattttgaaa cagtcattgg actagaagtc cacgtcgagt taaaaaccaa ctcaaaaatt
     8041 ttctctccag cgccagctca ttttggtgca gaaccaaaca gcaatacaaa cgtgatcgac
     8101 tggggttatc caggcgtgct gccagtaatg aataaagctg cgctggaatt cgggatgaaa
     8161 gcagctttgg cattaaactg tgaaatctct aaagatactc attttgaccg gaaaaattac
     8221 ttttacccag ataatccaaa agcatatcaa atttcccaat ttgatcaacc aatcggtcat
     8281 gacggatgga tcgaaatcga agtagaaggt aaaaagaaaa aaatccggat cgaacgtgtc
     8341 catttagaag aagatgctgg aaaaaacatg catggtattg gcggttattc ttacgttgat
     8401 ttgaaccgac aaggcacgcc tttgatcgaa atcgtttctg aagctgatat gcgttcacca
     8461 gaagaagcat acgcttatct agaagcattg cgatcaatta tccaatttac agaagtcagc
     8521 gatgtgaaaa tggaagaagg ctctatgcgc tgtgatgcca atatttctct tcgcccttat
     8581 ggacaagaag agttcggtac gaaagcagaa ttgaaaaact tgaactcttt atctttcgtg
     8641 aaaaaaggtt tagcttttga agaaaaacgt caagcaaaag ttcttctttc aggaggagaa
     8701 atccaacaag aaacacgccg ttttgacgaa acaacgaata aaacagtttt gatgcgtgtc
     8761 aaagaagggt ctagtgatta tcgttacttc ccagagcctg atatcccgaa attcgtgatc
     8821 gatgacgaat ggattcaaaa agtaaaagct agtttgcctg agatgccagc ttctcgtcgc
     8881 gaacgctata tccgtgagtt tggacttcca gagtatgacg caatggtatt gacacttaca
     8941 aaagaaatgt ctgatttttt tgaagctgtt ttggaaaatg gtgcagatgc caagcaagca
     9001 tctaactggt tgatgggtga agtttctgct tacttgaaca gtgagaaact ggaattagct
     9061 gagacaaaat tgacacctga gaacttagca ggaatgattc gtttgatcgc cgatggaacg
     9121 atcagctcaa aaatcgccaa aaaagtcttc cgtgaattga tcacaaatgg cggagaagcc
     9181 caagaagtag ttgaaaaaaa tggttgggtg caattatctg atccgaacaa actactgcca
     9241 atcattaatg aaattttgga taacaaccaa caatctgtcg atgatttcaa aaatggaaaa
     9301 gatcgtgcgg ttggcttcct agtcggtcaa atcatgaaag caactaaagg acaagcaaat
     9361 cctggtgtcg tgaataagct acttaaagat gaattagcga aacgatagag gctgaaaatg
     9421 aaaaaagcac gcgtaattta taaccccacc tcgggaaaag aattattgaa aaaaaatttg
     9481 gcggatatcc tacaggcatt agaagaagca ggatttgaag ccagcgccta tgcaactaca
     9541 ccagaaccag attcagccaa gaatgaagca tgtagagtcg cagaactagg ttttgatcta
     9601 gtagtggcag ctggtggcga tggcacaatc aacgaagtgg ttaatggaat cgctccgctt
     9661 gaacaccgtc ctaaaatggc catcatccca gctggtacaa cgaacgacta tgctcgagca
     9721 ttaaaaattc ctagagataa tattaaagca gcagcagaag tgataaaaaa acaccaaacc
     9781 gtaaaaatgg acatcggcct ttctgataac agttacttca tcaatatcgc tgcaggtggc
     9841 tatttgacag aattgactta tgaagttcca tcagaactaa agagtatttt tggttatctt
     9901 gcttatcttg cgaaaggagc agagatgctc cctcgagtaa agccaatcaa aatgcgcttg
     9961 aagtatgatg aaggggaata tatcggaaat gcttcgatgt ttttcctagg gctgacgaac
    10021 tctgttggag gattcgaaca gatcgcgcca gatgccaaat tggatgatgg gaagttttct
    10081 ttaatcattg tgaagacagc aaatatcttt gagatcttgc atattgcagc actcatgctg
    10141 aatggcggtc gacatgtcga tgatccaaga ataatctata ccaaaacaag cagtttgttc
    10201 gttgaaacag aagatgatat caatcgacca gtaatgatca acttagatgg tgaatacggc
    10261 ggggaagcac cgatgcattt ccaaaatctt catcaacaca ttgagttctt tgccaatacc
    10321 gatcagattc ctgatgaagc aatcacaggt acagatgaag aagaattaga ggttgtcagc
    10381 aaagaattcg taaaagaagt cgaacgttta acggatgaag atatcgacgg tgacggaaaa
    10441 atagcagacg aaacacctaa tcaaaagaat taatcaaaag ccggtgctga acgtagtaag
    10501 gtcagtgccg gttttagttg tgtaat
//
LOCUS       DAYYBG010000091        10496 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000091 DAYYBG010000000
VERSION     DAYYBG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.092"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..385
                     /locus_tag="ACHAUN_002287"
     CDS             236..385
                     /locus_tag="ACHAUN_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8460961.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            396..1910
                     /gene="dltA"
                     /locus_tag="ACHAUN_002288"
     CDS             396..1910
                     /gene="dltA"
                     /locus_tag="ACHAUN_002288"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8460962.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1907..3112
                     /gene="dltB"
                     /locus_tag="ACHAUN_002289"
     CDS             1907..3112
                     /gene="dltB"
                     /locus_tag="ACHAUN_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8460963.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3157..3390
                     /gene="dltC"
                     /locus_tag="ACHAUN_002290"
     CDS             3157..3390
                     /gene="dltC"
                     /locus_tag="ACHAUN_002290"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8460964.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3393..4661
                     /gene="dltD"
                     /locus_tag="ACHAUN_002291"
     CDS             3393..4661
                     /gene="dltD"
                     /locus_tag="ACHAUN_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8460965.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4802..5884
                     /locus_tag="ACHAUN_002292"
     CDS             4802..5884
                     /locus_tag="ACHAUN_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8460966.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5884..6687
                     /locus_tag="ACHAUN_002293"
     CDS             5884..6687
                     /locus_tag="ACHAUN_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8460967.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6757..7497
                     /locus_tag="ACHAUN_002294"
     CDS             6757..7497
                     /locus_tag="ACHAUN_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8460968.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7508..8512
                     /locus_tag="ACHAUN_002295"
     CDS             7508..8512
                     /locus_tag="ACHAUN_002295"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8460969.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8557..10401)
                     /locus_tag="ACHAUN_002296"
     CDS             complement(8557..10401)
                     /locus_tag="ACHAUN_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460970.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3385 a         1721 c         2112 g         3278 t
ORIGIN      
        1 ccatttattt ggaataagag gttgtgacac gatttttgtc acaacctctt tattttttat
       61 attttttaaa agtacgagaa attataaaga aaaaagcagt gatttgatgt gctattttta
      121 taaaagctat gtgacaaaac tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt
      181 tctttttgtc aaaattagtt acactttgtg tgaggttata tgaggaggga atccaatgaa
      241 caaatggcgt tctaatccaa aattgaatta ttggggaaaa ttcatcattc gaacaatttt
      301 ttatagtgca gtactgctgt tcctgattta tctgtatcac tataaaaata ttcaaggagg
      361 aacttttatc tataacgaat tttaggagga agagtatgga aatcaaaacg attattgaag
      421 caatcgatga ttggggaata aatgaacctg atcgagttgc ttatcaggcg gatgagacgc
      481 atacttttgg tgaattgaaa caagcatcag acgcacttgc ttattattta gaaaaaaagg
      541 tagaaggcga tggaccaatt gtcgtttttg gcaatttgga gtttgaaatg attgtctcat
      601 ttctaggggt tgtcaaatct ggccatgcct atttgccgat tgaagagcat acacccaagg
      661 agcgtatcct atcgatcttt cgggtggcta aaccatctat ggtcatcagt atcggggact
      721 ggatagaaga tattccttcg gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga
      781 agataccagt aggattttat cccagaaata gcgtaaaagg tacagaaaat ttttatatta
      841 tttttacttc tggtactact ggagaaccaa aaggcgtaca aatcagtcat gataatctag
      901 tcagctttgt tcaatggacg atggaagact ttgggatcaa gcctggtatg catttcttag
      961 cccaagcgcc attttctttt gatttatcgg tattttctat ttatcctgca ttagtttctg
     1021 gcggaatgct gaagccgtta aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga
     1081 cattgccagc cttgaagttg aatgtgtggg tttctactcc atcatttatg gatatttgct
     1141 tgatggaacc tacattcaat ggagaaaatg tgccggaatt ggacatgttt ttattttgcg
     1201 gagaagaatt gacgaaaaaa actgcagaaa gtctacttga tcgattccca aatgcacgga
     1261 tatataatac ttacggtcca acagaagcaa ctgtagctat ttcaagtatt cagattgacc
     1321 agcatgtact agatagttat gaccgactac cgattggcta cgtaaaaaaa gacacgaaag
     1381 tttctattgt ccaagaaggt aaacctgttt caaaaggaga aacgggtgaa atcattattg
     1441 caggaccaag cgtatcaaaa ggttacctga acaatcccga aaagacagct gctgcgttct
     1501 tttgtcaaaa tgacttagca agttaccata caggggatgc aggtagacta gatgaatatg
     1561 ggcttttatt ttatgaagga cgtatggatc agcaggtcaa attacatgga taccggattg
     1621 aactcgggga tatcgaacat tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg
     1681 taccaaaata ccaaggaaca aaagtacagc agctggttgc ttttgtcgta ttagaaaaca
     1741 aaacgaaaca gccggatttt cagttgacta aatctatcaa agaacaatta ctagaagttg
     1801 taatggatta tatgattcct cagaagttcc attatgtcga tctgctgcct cagacagtta
     1861 atggaaaaat cgatcgaaaa aaattaacag ctgaggtgaa tcctgaatga tgttacctca
     1921 tatgatccct tatgcagagc cgttttattt ttttctttta gccattgcgc ttgtaccgat
     1981 cgttctatct ttattgatat gggaaaaaag attaccagtt tatcaatcag cagtgacact
     2041 tttcttttta tttgtcagct ttggaggtaa ttattggcaa cagggagcgg cgctgattgg
     2101 gtatgttttg tggcagacga tacttgtatg gggctatttt gtttatcggc aaaggaaaaa
     2161 tcagacagga tggttttttg ccgccgttat tttagcgata attccactgt ttttaacgaa
     2221 agtgactcct ttttttgaac atggaaaagc atcgttactt ggttttttag gaatctctta
     2281 tttaaccttt aaatctgtac aaatgatcat ggaaataaga gatggcatga tcaaaacata
     2341 taagccattt cgttatatcc agtttctgct gttctttcca acaatctctt ctggaccaat
     2401 cgatcgctat aggagatttg aaaaagatca gctccatccg cctgttcgtg ataaatatgt
     2461 tgatcttttg ggaaaaggcg tccaaaatat ttttgtcggt tttttatata aattcattat
     2521 cggttattat tttggtcaag tactgcttcc tgtagtggga cgcattgcct tacagcatgg
     2581 tggtatttca tgggcattag taggttatat gtacgtgtac agtatgtatt tgttttttga
     2641 ttttgctggc tatagcttat ttgcagtcgg tacaagctac atgatgggat atcagacacc
     2701 aatcaatttc aataaacctt ttttatcatg gaatatcaaa gaattctgga atcgttggca
     2761 tatgacgctg tccttttggt tccgtgatta tgtatatatg cgattgatgt tcttcatgat
     2821 gaagaaaaaa ttttttaaaa gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt
     2881 tcttctaatg ggtatatggc atggattgac gtggtactac atcgtatatg gactgtatca
     2941 tgcttgtctg atctgtctga cagatgcatg gatccgattt aagaagaagc ataaagagca
     3001 gttgccttct aacaaattta cgcatggctt agccgtattt ttgacatttc aagcagtatg
     3061 ttttagcttc ctgatttttt caggattttt agataaactt ttttttagct aacgaatagt
     3121 gacatattaa tttatataac aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc
     3181 ttagaagaaa taacaggaac agatgaagta agaaaaaatc gtgatattga tctatttgat
     3241 gaagggttgc ttgattcttt agcatctgtt caattattag tcgagctaga cggagagctg
     3301 gatatccaag tccctgtatc tgaattcgag cgagaggatt ggtgcacacc gaataaaatc
     3361 attcaacaag caactgcatt gaagggttga gtatgaaaaa gaaacttttt gctattttcg
     3421 gcccggttgt tttagctgtt ggcttactgt ttgtgttttt tacctcctcc tatcaagtag
     3481 atatctctaa tccagaaacg attagacgtg cctcttcttc catgtcagtg aacgtgttga
     3541 aaggagatgc gataaaaaat tatgcattat ctgaaaaaca atatattcct ttttttggct
     3601 caagtgaatt aagcagaatc agtccatttc atccgtctgt tctggcagaa aaataccagc
     3661 gaaactatcg tccatttttg ctgggagcac ccgggacaca atcgttaagc caatatatga
     3721 tgatgcgttc tgcaggagat gctatgaaaa acaagaaagt cgtctttatt atttctcctc
     3781 agtggttcgt taaaaatggt gtaaaaactg attactttaa tacgtattat tctgaacttc
     3841 aaacatacga ctggttgttt tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta
     3901 gaagattatt aactttctca aaagtaaaag agaatgatac gttgacggcg attctgcaaa
     3961 cgatcaaaaa agggaagcta cctttacctg aatctttgaa tcagctgcgc agccaatgga
     4021 acatgctgaa gagagaggat gaagtattca gcaatattgg gttgaaagat cgacagcaaa
     4081 aaatcgatca tgagtcaaaa cgattaccaa aacaatacca agaaacagaa ttatccattt
     4141 tagcaaatca aattggagaa cgagaaacga cgaataatcc attcgggttg aaaaacgatt
     4201 tttatacgca tcgtatccga gcccacgagc cagagctcaa acagtcacaa aaaaattggg
     4261 attatcgttt ttcgccagaa ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa
     4321 accataacga agtattgttc atcatcccac cggttaacga gaaatggagc gattataccg
     4381 gactatctca agaaatgtta caaggatttg ccaaaaagat caaatttcaa ttaaatagcc
     4441 aaggattcaa tcgaatagct gattttgtca atcaagcggg taccaactat tttatggaag
     4501 acacgatcca tctaggctgg aaaggctggt tggctgcaga tcaacagatc agaccgtttt
     4561 tagaagaaaa tcatattact gctagtaagt atcatcttga cgatgctttt tttagcaaat
     4621 cttggcaaca tcaaatacca gataaactac aattaaaata aagaaaacag gcgagtgttg
     4681 ctcgcttgtt ttctttattt ttgaatattc tgaataaaag ataaaaatac caagtttgcc
     4741 tgaacaaaaa tcataagcaa tggtataatc acaatatgaa tatagaaatg ggggaaaaca
     4801 tttggataca aaagaagaac agctacttat tgatttaact tctgcaaaag gagtacctgg
     4861 aaatgaagaa gaggtaagag aagtattcag agaatatgcc aaaccattcg ctgatgatat
     4921 tttttatgac ggtttaggca gtgtgattgc caaacatggt gctggcggag gccccaaggt
     4981 ctttatttct ggacatatgg acgaagttgg ttttatggta acaaaaataa ccgaaaaagg
     5041 attcttggaa ttccaaactc ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt
     5101 tgaaatcaaa acacgtgaag ggaaaatcgt ccacggtgtg attggttcca aaccaccaca
     5161 tgttttaaca cctcaagtaa gaaataaacc ttatgaaata aaggatatgt tcattgatat
     5221 tggcgcatca agccaagaag aagccaaaga gtggggcata cgtcctggag atatggtcac
     5281 tccatatatt gaatacaaac gaatgaatgg cagtaaatat ttactggcaa aagcatggga
     5341 taatcgtatc gggacagctg tttctctgcg agtattagag aatctatcta aagaagcaca
     5401 cccaaatgtt ttattcgcag gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg
     5461 aacaagtaca caccttgtaa atcccgatat tgcttttgct ttagatactg gtacagcagg
     5521 agacactcca ggaatgacac caaaagaagc agattcgatt ttaggaaaag gcccacagat
     5581 tttgattttt gatgcatcta tgatcccgca taaaaaatta ttgaattttg ttattgatgt
     5641 agcagaggaa atggatattc cattccaata tacagtgatt tctggcggag gaactgatgc
     5701 aggacaaatg catttgacaa gagatggtgt tccgtctgta gccattaccg ttcctgttcg
     5761 ttatctgcat tcccatacat cagtgattca tgaagatgat tatttgaata cagtgaaact
     5821 agtcacagaa ttagtgaaac ggcttgatag tgaaacagtt gaaaaaattc ggagttatga
     5881 ataatgaata atagaatacc tatttctgaa tatcggaaaa aagcacgagt ttctttggaa
     5941 aatcaatggg gaatcaatac ttggcttgtt ttcttatcta taatatttac gagcgtcatc
     6001 cagaactttt ttgacggttt cagtcctttt ccggaagaaa gcatgcagca gcacttattg
     6061 agttttctag tcgataattt acttttattc gcattcaact atgcattgtt ttacattgcg
     6121 ctagtagttg tacggggtgg cagaccaaaa agtaaccttt tattttcagt tttccaaaga
     6181 aagtattttg gttcgatact tggcatcaat ttgatcaatt cagtcatcaa ctggctactc
     6241 aacagtttaa tctttctgcc catgttttta atcagcggag tgagtactta tatgcagctg
     6301 cttttcagtt caaatgcggt acctacgggt gtcatgccag aagattttct tgatatcagc
     6361 tttgttttat cgatgatagg gatgattttt gtttcagcac tcatcatgag tattgttggc
     6421 ggtttattcc aatttgctgc atggacaaag ttcgactatc ctgatttgtc ggttattcaa
     6481 tgtttgaaat atggctggtt tttattgaaa gatagaattg gtacgtatat tttgttgcag
     6541 ctgtctttta tcggctggta tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg
     6601 gtcattgttt atgtgaatgt gtcagttgca gaattttatg aacaagcacg gattgaaaaa
     6661 ggaggtccag acgcctattt tacttagcat gtaagcagtt tattcatcaa aaaaataact
     6721 tttgtggtag gataaatctt tgagggggaa tgtacgatga aattaactgt tttaggatgc
     6781 ttaggagcgt atccttataa aaatcaaggg acaacaggtt atttgcttca atctgacggg
     6841 ttcaatttgt tgatcgatgc aggtagtgct acgctgatca aattacaaga acaccttgat
     6901 ccacttgatc tagatgctgt gatccttagc cattatcatc atgatcatat tgctgacctt
     6961 ggtgtattac agtattactg gcaacttcat cctaagagaa atccggagac attgctttca
     7021 atttatggac acacaaaaga ttccattcac tttgaagaat tgacgttgga aaatgtctca
     7081 aaaggacatc cttattttga agctgaagaa ttaaaaattg gtccttttct gattactttt
     7141 atggaaacaa tccatccagt agtatgttac gccatgcgta tcgtagaaga aacaacgggg
     7201 aaagtattcg tatttacagg tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa
     7261 gatgctgatt tgtttctagc agatacctat ctttttgaag gaaatgaaca tcatcatgcc
     7321 catttcactt caaaagaagc tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta
     7381 cttacacacc tacctcagtt tggtgattta gaacaattga aaaatgaagc gcaaaaggca
     7441 gcaggcaaag ggattgatat agaacttgct gcagttgaca aagtgtttaa catttaggag
     7501 gaagaacatg atttttgtac caaatgaaaa ccacgaccca agaatcaatt tagcaattga
     7561 acaatatttg ctgcaagaaa tgcctttaga tgaacctatt ttgctctttt atatcaatga
     7621 accttcaatc atcatcggca gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt
     7681 ggaagaaaat ggcattcatg ttgtccgtcg cttaagtgga ggcggagctg tctaccatga
     7741 ttttggaaac ctgaacttta gttttatcat gcctgatgat ggggattcat tccgtgactt
     7801 tgcgaaagta acacagccaa tcatccaagc acttcatgaa ttgggtgttg ctggggcgga
     7861 attgaaaggc cgaaatgatc tagtcattga tggcatgaaa ttttctggca acgcgatgta
     7921 tgctaccaac ggtcggatgt ttgctcatgg aacaattatg ttcgactctg atatcaatga
     7981 agtagtcaat gcattaaaag taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat
     8041 ccgttctcgt gtaacgaata tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac
     8101 agaagaattt cgtcaagaaa tcttgttgaa aattttcggc gtagagtcag tagaagaagt
     8161 aaaaacgtac gaattgactg atgaagattg gaaaaaaatc aatcgaatct ctgaggaata
     8221 ttatcgaaat tgggattgga attacgggaa gtctccagat ttcaatttct ctcgccaaaa
     8281 acgtttttct atcggttcga ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga
     8341 agcaaaaatc tttggcgatt tcttcggact aggagatatc tctgacgtgg aaaaaaaatt
     8401 ggttggacag aaatatgata aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa
     8461 gaaatatttt ggtgcgatag aaccagaaga attgcttacc ttactttatt gaaaataaaa
     8521 aatagcggaa tccacatata tggattccgc tattttttat acaggattcg ggtaatttcg
     8581 agagagttga acagtgatcc gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt
     8641 gtaacgatcc gctacgtctt tcatcatcat taagtcaaaa cgttcctgat catctaattc
     8701 ttctgtgatg cctcgactaa ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat
     8761 gacgaaatgt tcctcttgtt cgtgtatcga caaataaatt tttcgataca gtggattttc
     8821 gatggcatga gtttctctaa aggaatgctc taaagcgtgg atcaaaagtt gagccagatc
     8881 gtcatcgttg ataggaagtt cttcgggaag atctgcttgg aaggttatct gaatattccg
     8941 tttggcagct aacttctggt aacgcgctaa gataagattt gtcacctggt ggttgcaaaa
     9001 agaacacagc atttcgaaat tttttgcttc ttctaatatc ttttccacat agtttttagc
     9061 ttttgtttgg tccgtatgca ttaaatcttg cagattctcc atctcttgga caaattccag
     9121 gcgaagtgta tttacttctt ggatatgctg tcgaagctgc tcataatgtt gctccaacaa
     9181 atcatttttg atttttaaga aatctacttg tctccgatct ttgtcggtat ttaagatgtg
     9241 atcgattaga ttgtagctga taataacgat tagaatgata atatataaaa tcgtagacca
     9301 attgatcgtt gtttgataga tacctaatgc cgtttggatg taaatcaagc aatggataat
     9361 ggctaccaag acgatccaag gtgtacaagc tacataaaaa cggttattct tatatgcttc
     9421 gccaatgcat gcaagtgaag tgactaaagt gcttataaca ctagcaatat tagcaatcgg
     9481 cattaattca ggaagttctg caggagtaaa agttgcataa agcagaacgc cgataaaaat
     9541 caaaagttgg aacgtacaaa aaattccgta ccatttttta tagtaaaaca ttcggctcca
     9601 atagtagcag atgatgaaag ctgacgttaa gataataaat aaatgagata aaaaagaatg
     9661 aacgaatggt tcaaaaagaa taccacttaa aatatcttct gatacagctt caaatcctaa
     9721 tgccaaagca aagcaactta agagaataag tgagtattca agacgttttt gtttatacat
     9781 caagtagatc gtatagaaaa aggtccctat agcgataaaa acagcgatta tcatcgtcaa
     9841 tacttgcggc actgattgtg aataaataaa actaatcaat ggtccagttg ttcctagata
     9901 aactttcggt ggcagtccag catagttctg ataaggagat gagactgtaa gggtcaattt
     9961 ttttccgata taatttgctg gtaaatctat cacagccaat gagagtccgg gattgctttc
    10021 ttcctgataa tctttccgtt ggtataaaat cttttcgcct agatatacag taatccattg
    10081 atggtttcct ttgattagta aatttgcttc gtccaacggt ttttcaagtg tcgtttccat
    10141 aatgaatgtt tcgtctttat ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt
    10201 aaaatcagga tcatcttttg tatagaaaac ccacgaatct tttatttctt tcatttgatt
    10261 gctgtccgca ggagaaaaaa taaaataaaa gaaataagca attcctgtta aaaataacaa
    10321 tgataatata atgattatgc tccactttat acgtaaataa aaagattctt ttttattata
    10381 tatgttatta aaaaaagaca taaaggtatt cctttctaaa acttgtaatg ttagggtaaa
    10441 cgttttttaa actaacgtca attatttata ataaaaaatg agataaagat gaggaa
//
LOCUS       DAYYBG010000092        10297 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000092 DAYYBG010000000
VERSION     DAYYBG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.093"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..256)
                     /gene="acpP"
                     /locus_tag="ACHAUN_002297"
     CDS             complement(14..256)
                     /gene="acpP"
                     /locus_tag="ACHAUN_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8460971.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(378..1379)
                     /gene="plsX"
                     /locus_tag="ACHAUN_002298"
     CDS             complement(378..1379)
                     /gene="plsX"
                     /locus_tag="ACHAUN_002298"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8460972.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1428..3464)
                     /gene="recG"
                     /locus_tag="ACHAUN_002299"
     CDS             complement(1428..3464)
                     /gene="recG"
                     /locus_tag="ACHAUN_002299"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8460973.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3581..5263)
                     /locus_tag="ACHAUN_002300"
     CDS             complement(3581..5263)
                     /locus_tag="ACHAUN_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8460974.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5315..5677)
                     /locus_tag="ACHAUN_002301"
     CDS             complement(5315..5677)
                     /locus_tag="ACHAUN_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8460975.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5947..6135
                     /gene="rpmB"
                     /locus_tag="ACHAUN_002302"
     CDS             5947..6135
                     /gene="rpmB"
                     /locus_tag="ACHAUN_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8460976.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6373..9042
                     /locus_tag="ACHAUN_002303"
     CDS             6373..9042
                     /locus_tag="ACHAUN_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8460977.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9497..10243)
                     /gene="truA"
                     /locus_tag="ACHAUN_002304"
     CDS             complement(9497..10243)
                     /gene="truA"
                     /locus_tag="ACHAUN_002304"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8460978.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2800 a         2224 c         1852 g         3421 t
ORIGIN      
        1 cttttatttg aaattattta agatggctgc tgatataatc tactgcgcca ccgactgttt
       61 caatttgttc agcgtcttca tctgaaattt ccgtaccgaa ttcatcttca agctctaaaa
      121 cgaattccat aatactaatt gaatcagctt ttaaatcatc tttgatgttc aattcatccg
      181 tcactttatc ggtatctatc tcaaaatgat ttgaaataat tttcgctact ttgttaaata
      241 cttcttcgcg agtcaacttt ttcacctcca agaatcatac acttgtcaat tacctacacc
      301 atggctaatt ttactggcat ttttcaggaa tgtccacctt tttttcaatg gaacggatcg
      361 cttcagatat ccaaatacta ttcttcgctt ttttcaaatt gttcgactaa ttgtccaact
      421 acatctgtct caagcattgt gtggatttgg cggatcgtat agcgaacagc atcggggcct
      481 gttgctccat gagttttaat gactggtgct ttcaagccaa acaaaacggc accgccatgt
      541 ttggaataat ccatttctgc tttcaaagaa cgtaagccgt cttttaaaag catagcgccc
      601 atttttcctt tgatcccttc atttagaata gcagatttta atagattcat catattcatt
      661 gctgttcctt caatagattt caaaacagcg ttgcctgtaa atccatctgt gaccactaca
      721 tcagccacac cattgagtag ctcacgagct tctacgtttc caataaaatt caagcttgtt
      781 tcattctgaa ggagttcata ggcttttttc gtcacttcac taccttttgt tgcttctgtt
      841 ccgttgttta agagtgcgac acgaggacgt ccgatacctc ggactttacg tgcataaaaa
      901 gaacctaaaa taccgtattg caacaaatgt tctggtttgt tttctgcgtt agcgccaaga
      961 tccagcatat cgaaaccggc accttctttc cctactactg gcaaggtaga catcaagcca
     1021 gggcgttcga tatttttgat acgtcctacg atgaacaacc ctgctgctag tagcgcgcct
     1081 gtattccctg ctgaaaatac agcatctgct tcaccgtttt tcactgcttg tgcagcaagg
     1141 accattgatg cagtcttttt tcgtcgaatt gcttttactg gctcatcatc actattgatt
     1201 ttttcgtctg tatggataat cgtgatgttt ttttcatttg ttaagtattt cttgatttct
     1261 gcttcttttc cgtaaagtaa gaattctact tctggaaaat cttgctgcgc taaagccacg
     1321 ccttctacaa ttgcttgagg tgcgtgatct ccgcccattg catctacggc aattttcatt
     1381 gtttccactc ctttttccgt catcagtata aagtatagca cagtctgcta atcgaaataa
     1441 cgattatcct gattttctat ttggaggctt tgcgctaaag gctgatactc aggaagcatc
     1501 tgccaatcct ttatttgcca aagggctgca gcctcttctc tggctacctc caaaacattt
     1561 ccatccgtca ccaagtcagc aatcgcaaat tgaggcagac cagattgacg gaagccaaat
     1621 acttcaccag gaccacgtaa ttccagatct ttttcgctta gtacaaaacc attggtggtc
     1681 tctgtcatga ttttcatccg ttccacacct agttcatttt taggattagc aactaagata
     1741 cagtaggaag catcacttcc gcgtccaaca cgtccacgaa gttggtgcag ctgtgccaat
     1801 ccaaatcgat ccgcatccat gatcagcatg acggtcgcgt tcggcacgtt tacccctact
     1861 tcaattacag tagtcgaaac taatatctgc atctggttgt ctttgaaggc ttccatgatt
     1921 acttcttttt cctgattctt cattttacca tgtaacaagc caacttcata ttttggtgca
     1981 aatagttcac gaagtttttc atagatttcc actgcatttt ttacatctaa tgcttctgat
     2041 tcttcaatca gcggacagat gacatacatt tgatgtcctc ttgccagttc ttttttcgtc
     2101 caatccagta cactgtccaa ttgaggtgtt cttacccagc gtgtttcgat cgggatacgt
     2161 ccagctggaa gttcatcaat gatagataca tccatctcac catatgctgt aatagctaat
     2221 gttctaggaa tcggagtcgc tgtcataaat aagacatctg gtcgccagcc tttttcacga
     2281 agtactttcc gctggttcac gccaaaacgg tgctgctcat ccgtaataac tagacctaaa
     2341 ttctgaaatt cgactccatc ttggatcaat gcatgtgtgc cgatcacgac gttaatttcc
     2401 ccttgctcta gtttttcaag gagctcacgt cgttcttttg ctttcgttga ccccgtaatc
     2461 aatgcgatgg ttacttcatt gggatcaaac agctgttgca agctttccat atgttgttgt
     2521 gccaagattt ctgtaggcac catcaacgca ccttggaaac ctgctgtaac ggtagcgtat
     2581 aacgcaatcg cggcaaccac tgtttttccg ctacctacat ctccctgcaa aagtcgctgc
     2641 atatgctgcg gactgcgaag atcccgacag atttcattcg ttacacgttt ttgtgcattt
     2701 gtcagttcaa aaggtagctt ttgagtaaat tgtttcaatt tttggacatc ataaaggatc
     2761 tccaaaccat tcgtttctgc tttttctgct tttttcaatc cttgaagacg catttgaaat
     2821 aaaaagaact cttcaaaaac gacacgtcgt ttggcttgat ggttttcttc gtggtctttt
     2881 ggaaaatgca tacttcttac agcagaagct cggtcaagca gtcgatattt ctccacaagg
     2941 ttgtttggca gattttcttc aatcaatgtg ccgtattctt gaaacccttt tttgattaga
     3001 tcaactaatg tcgtttgacg aattgatttg ttgacgtgat atatcggcga aaagtcatcc
     3061 attgtttgag aacctaaaat cttcatcccg ttcaaggctt tacgctttgc atcccattta
     3121 ccgtatacgg cgatttcctc cgataaaatg actttttctt ttagataagg ttgattaaaa
     3181 aaagaaacgt tgaacacatc atgttcttgc atcatacgaa attgcaatcg gcttttttta
     3241 taaccaaatc gattcatcac aggaggcgag actacgattc ctttcaaagt aaccttttct
     3301 tgatcctgga tctcgtttag atttctttcc tgaatatctt cgtagcgaaa aggataataa
     3361 gtcagtaagt cttcaatcgt ttggatgccc aagctggcaa gactagctgc tcttttttct
     3421 ccgactcctg ccagcatagt gattggatca tctaaagaac gcataaattt ttcctccctc
     3481 ctttaacaaa aaaaggactt ggtcagcgat catacctgat cactaaccga aggcctttta
     3541 tgaatgaata ctgtgttaga aagatattct gacacagaaa ttattcagca gaaaatagat
     3601 aaggatagac aggctgatct ccttggtgga tctcgatttc caaatcagga tgtttttctg
     3661 ttagtttctc agcaaattca tttgcttgtt caactgttcc ttcatcacct gccaagatcg
     3721 ttacgatctc tgtgtcctca ttgatcattt gttctagtgt acttaacgct gttgctgtga
     3781 tatctgggtt agagatcagg atttttccat cgatcatgcc caaaaagtct ccttctttga
     3841 tctctacacc gtcaattgag gtatctctga tcgcgtgagt gacttgaccg ctcacaacgc
     3901 tagacaacat ttctgtcatc gtttctttgt tttcttctaa agaagcttga tcattgaatg
     3961 aaagcatagc tgtcaatcct tgggaaatcg ttcttgaagg aacaactgct actgggatat
     4021 ctgctacttc tgctgcttga tcagctgcca tgaagatgtt cttattgttt ggcaagacaa
     4081 tcactttatc tgcgtttact tcttgaatcg ctttaacaat gtcttccgta cttggattca
     4141 ttgtttgtcc accactgata acataattag cgcctaaact ttcaaacagc tcttggacgc
     4201 ctttaccagc agcgatcgct acgataccat acgggatacg tggtatttct tttttcgcaa
     4261 actcgttcac ttcttcatcg tgttccaaaa tcgtctcatg ctgcaagcgc atgttgtcga
     4321 ctttgatttt gactaatgaa ccgaattttt gtccataatt caagacttct cctgggtttt
     4381 ctgtatgcac gtggactttg atgatctcgt catcattgac cacaagtaaa gaatctccta
     4441 taccatctaa atagttacgg aattcttcgt aatcgaatgt actatcgact gtaggtcctt
     4501 ctccgatttt taccatgatt tctgtacagt aaccaaaatg gatatcttct gtcgccattt
     4561 gaccttggac actgcgatga tgttctgcat tgaccatatc atccatttct gctggagaag
     4621 gttcatatgt ttcatccgct tggaattctc cattcaatgc acttaaaaag ccctcataaa
     4681 caaacagcag tccttgccct ccactgtcta cgacaccgac ttcttttagg actggaagca
     4741 agtctggagt tttagctaaa gcacgctttg ctccttttac aacagctgtc attacttcaa
     4801 cgacatcatc agtacttttt gctttctttt ctccagcttt cgctgcttct ctagctactg
     4861 tcaaaatcgt tccttctact ggtttcatta ctgctttata cgcagtttca actccatgag
     4921 tgaaggctgc agcaagatca gaagcattta atgagacaac atctggaatt tgcttagaaa
     4981 atccacgaaa caattgagac aaaataacgc cggaatttcc gcgcgcaccc atcagcaatc
     5041 cttttgaaag cattgctgct aattctccta ctttttctga ggttgaatct actactgcct
     5101 tagctccact agtcatagat agattcatat ttgttcccgt atcgccatct ggcacaggga
     5161 atacatttaa tgaattgacg tattctgcat ttacttgcag acgattcgca cctgcctgga
     5221 ccatttcctg gaactggctt gcacttattt ctgttacact cacctagata atcctccttc
     5281 agatcgactg ctgagttacc agccagtctc ctgcctagtc tggcagtaca cgaacacctt
     5341 gtacaaagac attaactgaa ttggcagtta cgcctaataa tgcctctaga ttgtatttca
     5401 ctttttcttg gacgttgcgg gaaacttcag agattttcgt accatagctg ataatcgtat
     5461 aaacatctac agcaatacca ttatcttctt ggcggacaac tactcctctt gagtaatttt
     5521 ctttacgcaa aatctcagta atattatctt taatttgatt tttgctggcc attcctacaa
     5581 caccgaacac atctgtcgcg gcgccaccaa caactgttgc aattacatca ttggtaattt
     5641 caatggttcc agctgacgtt tgaattttta cagccatgaa tgagcctcct taacgggtaa
     5701 aaaactccca tttttcttct atcatatttt atcatagcgc aacttgaaat aaaaggcagt
     5761 tacccaaagt actcaaaaac cttgcaaaat ttcaggtata accttgttta atttacaatt
     5821 attcttctca attgtcaatt gtatctatga aaatgtactt gcaaattccc attctatatg
     5881 atatgttagt ctggtatgag ctgaattatt ctattgctcc aaatcaaggc aaaggaggaa
     5941 attaccatgg caaaagtttg ttactttaca ggtcgcaaga caaaaagtgg aaacaaccgt
     6001 tcacacgcta tgaactcaac aaaacgtact gttaaaccta acttacaaaa agttcgcgtt
     6061 atggtcgacg gaaaacctaa aaaagtttgg gtgtcaactc gtgctttgaa atcaggaaaa
     6121 gttgaacgcg tttaattgaa tgatacttgg accgttcaga tagtgaacgg ttttttattt
     6181 aataaaaatt tttaagggtg ggacttttga ttcctaccct ttttttgcgt ttatttttgt
     6241 tatacgaaca aattatgaag ttatttattc taaattttat ttttttgtga aaactagact
     6301 ctttttaagc agagaaaaac aaggatatga taaactgaat acaacttcat agaaatttgg
     6361 tggaaaataa atatgaaaaa aaatttaaag tcattcttta gagaaaacgg acgctacgca
     6421 gcagctagtt tcctgattcc gtttttgatt ttagcacttg tttatctaag tatcggaatc
     6481 tatcctggaa gcagcagaag tattttggca agtgatgcct tttcacagtt ttctaatttt
     6541 catgccagct tccgaaatgt tcttcttgga aagcaaagta ttttttatac atggaatgct
     6601 tctctaggat tgaattattt atctttaatt tcttactatc taggcgggat cttcacgcca
     6661 ctggtgttct tttttccaaa ccagatgatg ccagatgcac tgtatgttct gactttgctt
     6721 aaggtcggct gtgccggatt ggctttttgg tttcttgcac gtacgttcaa gattcctcgc
     6781 tgggggcatg ttgcacttag cgtcagttac gcattgatct catttatcac tgcacattcg
     6841 gagatcatca tgtggctgga tgcatttttt tatcttccat tagtgatttt ggggattcat
     6901 cgtgttatgg atcttaaacg gccgaaactt ttatttatca gttatttact cttgttttta
     6961 tccagttttt atatgggttt tatgattggc gtattttctt tcttatattt tatcgcccgt
     7021 ctattgacaa actggtcaga atataaaaaa acgattgttc cttatggtat cacttcttta
     7081 ctggcaggcg gcgcttcaat gatcattgta ttgccagcag tgctggatct tcggtcaaat
     7141 ggcgaggcac tgagtcagat cactacgttg aaaacagaag cgacttctta tctggatcta
     7201 atcatgaaaa atatgattgg tgtttatgac acgacaaaat acggatcgat tccgttcgtt
     7261 tacgcaggtc tgctaccatt agtcctctgt ctgttttatt ttgccagtcg gaaagtaccg
     7321 ttgaaaaata aatttttgta cggcagtctt ttcgccatac ttatcgcaag tttttatctt
     7381 gttccattga atttgttctg gcatggtatg catgcaccga atatgttttt attccgttat
     7441 gcctatctgt tctctttctt agtgctcctg cttgcaggaa aaggatggga aaagatagaa
     7501 caagacgatc gtgggctgtt gttaggaatc attttggtcg tagctgctct ctttgcggtg
     7561 gcatggggca tcaaacctga aggaagctac acctatgtga cgatgacttc ctttgtattg
     7621 acagtggttt tcttagggat ttatgctttt gctgtgggat tttatcagca taaaaaactt
     7681 tcattcaatc atctgagtat attgttatta ttgatcatgt ctgctgaagc actggtcaat
     7741 acgaacagta tgatccatgg cattcttgat gattggaatt atgcttcccg cagtttatat
     7801 actgatcctt atcctgactt gaaagctctt gtaaatgaaa caaaaaaaga ttccgactcg
     7861 ttttaccgct tggaaaatct ggatccagta tctgcaaacg attcgatcaa ctatggttac
     7921 agtggtatca gtttgttttc ttctataaga aatcgtaatt cttcctctta cctggataaa
     7981 ttaggtttcc gttcaagagg gacaaatttg aatatccgtt ataacaataa tactttgctg
     8041 atggatgggt ttacaggaat caaatataac atttctaaaa acgaaagcac gttcagtaaa
     8101 tatggcttcc gccaagctgg tcaaagcggc gactatacac tgtatcagaa tgtcaatgca
     8161 ttgccactag catttactgc accgttgtcc gttaatgatg ttgagcagcc agtgacagat
     8221 aatttgacca gtcagacaaa cttgatcaat acattgtctg gtctaaatga acagtatttt
     8281 acattctata cacctacttt gaagaatcag ttgaatacga cgatcacaaa gacaacgact
     8341 ggcgtcactt actcagaaac agcagataac cagccaaaag agctcacttg ggaagtccat
     8401 gtgcctgcaa atacacaagc ttacttaagt ttgttcccaa ccaattttgc gcaattagaa
     8461 agttctagtg cgacgatcac tgtcaatggt acatcgcgaa aaacgcaaat caacatttct
     8521 ggtcaatact atgatcttgg ctattatcct caagagacaa cagtgacatt tactgcaagt
     8581 ttttatggga cgaaagaaat aagtttcatg gagcctaaag tcgttggtct agatgttgta
     8641 gcctatcaag ccgcaatgaa tcagatcaaa gaaaaaggtg tcgaaatgga agccactggc
     8701 agaaaagctt ctgggactgt aaataccgct gaagattcca tgttggtgac aactattcct
     8761 tatgacaaag gatggaaagc taaaattgat gggaaaccag tcaaggtcag caatttcaaa
     8821 gacgcattct tgatgataaa agttccagca ggtaaacata ctgtcacttt cagttacttg
     8881 cctgaaggat ttacgatagg tgcaatcctt tttgtcctat gtattgcatt gtttattctt
     8941 tatgtctggt accttgaccg caatcgcccc ttttatggca cgattgatcc agcaatagaa
     9001 gaacaaccac gaaacacaca cagaagaaga cgtagaaaat aaagaaacga tggaacctgt
     9061 tcagactgtc cgcaccaagg gcaatcaaaa caacgttcca tcgttttttg gttacagaaa
     9121 aaataggtaa caaaaagatg attcacttat caacgcctcg tccacaagtc acgatattca
     9181 tcaaagatga gaagctgttt tttgaaattg ctctttgtga ctcagagtta aatgacaggc
     9241 tcacgacctc tttcttaaac ggtgttcaat cagctgacct tcggtattag ctcaacaaac
     9301 ggcaaaatat gaggagctat tttcccgttt gtttctctaa tactcaggtc aaaacgctga
     9361 tttcacgacc tcttccttaa acggtgttcc agaagtagct tcttcgaaaa taagccgaaa
     9421 ttttgcaaaa ttttgagaag taattttcgt aaataccttc ttatttcttg aagctgacca
     9481 cttctatcat aacctcttat tcatatttca cttcatacaa ataaagtcct tcagggtgtg
     9541 ctgtaggtcc tgctgcatta cgatcttttt tcgcaataat atctgggata ctatcttcag
     9601 gcaatcggcc attgccaatt ttcaaaagcg ttcctactaa gatccgtatc atcttataca
     9661 agaagccatt tcccctaaat gtaaacaaca attcgtcttt tgtttcattt acctccatct
     9721 tagcctcgta gatcgtacgt attttatcct ctatcgagct tcctgatgca caaaaagagg
     9781 tgaaatcgtg agtccctaat aaatcaggaa gcgcccgctg aattttttcc aaatcaagtg
     9841 ggtaaggaaa ataacttgca taatggcgtc gaaaaggact gcgtggtttc cctatatcca
     9901 ctcgaaattg atacgtcttt tctttgacca aatatcttgc gtggaagtct tctgaaacaa
     9961 tttctacttg tcttacagca atatcctctg gtgactgggt atctagcgcg aaacgcattc
    10021 gttctaacgg acgctcctct ggataatcaa aatggattac ttgcccgatc gcatgtactc
    10081 cggcatctgt ccttcccgaa ccaaagaccg tgatttcttt tccatttgcc attttttgta
    10141 atgttttttc tacttcttct tgaacagtac gcccatttgg ctgtttttgg aatccattaa
    10201 aatttgttcc gtcgtacgca atgattgctt tgtaacgtac catgtttttc tctcctttgt
    10261 tcctaaaagg ttatctggaa taatgatttt tctataa
//
LOCUS       DAYYBG010000093        10211 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000093 DAYYBG010000000
VERSION     DAYYBG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.094"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            388..657
                     /gene="rpsN"
                     /locus_tag="ACHAUN_002305"
     CDS             388..657
                     /gene="rpsN"
                     /locus_tag="ACHAUN_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8460979.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            672..851
                     /gene="rpmF"
                     /locus_tag="ACHAUN_002306"
     CDS             672..851
                     /gene="rpmF"
                     /locus_tag="ACHAUN_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8460980.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            883..1260
                     /locus_tag="ACHAUN_002307"
     CDS             883..1260
                     /locus_tag="ACHAUN_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460981.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1281..1430
                     /gene="rpmG"
                     /locus_tag="ACHAUN_002308"
     CDS             1281..1430
                     /gene="rpmG"
                     /locus_tag="ACHAUN_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8460982.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1454..2437
                     /locus_tag="ACHAUN_002309"
     CDS             1454..2437
                     /locus_tag="ACHAUN_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8460983.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2467..2589
                     /locus_tag="ACHAUN_002310"
     CDS             2467..2589
                     /locus_tag="ACHAUN_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8460984.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2607..4130
                     /locus_tag="ACHAUN_002311"
     CDS             2607..4130
                     /locus_tag="ACHAUN_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8460985.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4154..4393
                     /locus_tag="ACHAUN_002312"
     CDS             4154..4393
                     /locus_tag="ACHAUN_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8460986.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4586..5149)
                     /locus_tag="ACHAUN_002313"
     CDS             complement(4586..5149)
                     /locus_tag="ACHAUN_002313"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8460987.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5242..5460)
                     /locus_tag="ACHAUN_002314"
     CDS             complement(5242..5460)
                     /locus_tag="ACHAUN_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8460988.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5649..6596
                     /locus_tag="ACHAUN_002315"
     CDS             5649..6596
                     /locus_tag="ACHAUN_002315"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8460989.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6563..6652
                     /locus_tag="ACHAUN_002316"
                     /pseudo
     CDS             6563..6652
                     /locus_tag="ACHAUN_002316"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6785..7192
                     /locus_tag="ACHAUN_002317"
     CDS             6785..7192
                     /locus_tag="ACHAUN_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8460990.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(7433..8773)
                     /locus_tag="ACHAUN_002318"
     CDS             complement(7433..8773)
                     /locus_tag="ACHAUN_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8460991.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(9049..9204)
                     /locus_tag="ACHAUN_002319"
     CDS             complement(9049..9204)
                     /locus_tag="ACHAUN_002319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8460992.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9211..9357)
                     /locus_tag="ACHAUN_002320"
     CDS             complement(9211..9357)
                     /locus_tag="ACHAUN_002320"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8460993.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9388..9591)
                     /locus_tag="ACHAUN_002321"
     CDS             complement(9388..9591)
                     /locus_tag="ACHAUN_002321"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8460994.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            complement(9501..9833)
                     /locus_tag="ACHAUN_002322"
     CDS             complement(9501..9833)
                     /locus_tag="ACHAUN_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8460995.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3404 a         1621 c         2009 g         3177 t
ORIGIN      
        1 tctattctta taatataagg aataatcaaa gttttaaaaa attggcgata aaccagttaa
       61 acataaaaga ttgaataaaa agttatagtt tctttcatat tgccgtttta agtagaggaa
      121 aagctactta aaacggtatt tgtgtttgat tggactaata aaagtgaatt cttatttttc
      181 agagcttaaa ctgcatattc ttttaaatga tttgattatc tctatgtacc gataaattcg
      241 ttttcaaata gttatctatt catatgagtt ttatttcatg ttatttattg ttgacaagag
      301 caactcttgt tggtaaagta tcagtgtcaa aacgtaacga ttacgatttg agatgtctaa
      361 atcaaaataa aaggagtata aaggtatatg gcaaaaaagt cgaaaattgc taaagcaaag
      421 aaacaaatgg cgatgattga aaagtacgca gataaacgtc aggaattaaa agccgcagga
      481 gatcgcaccg ctctcgctaa attgccaaga gattcaaatc cgaatcgtct gaggcttcgt
      541 gatcagacgg atggtcgtcc aagagggtat atgcgaaagt tcggtatgtc ccgtattaag
      601 tttagagaac ttgcccatca agggttgatt cctggagtaa aaaaagcaag ttggtaaaag
      661 gaggaaatat catggcagta ccagcaagaa aaacatctaa ggcgaagaaa cggttaagaa
      721 gaactcatca aactgtagta aaaccagaaa tttcatttga cgaagcgaat ggtgattata
      781 gacgtagcca tcatgtatct ttaaaagggt actataatgg aaagaaagta attaaaggag
      841 cttcaaaata gtttaatata taacttttag gagggaatca aaatggaaat tatttaccca
      901 cctttagttg aagaaggatt aaagtattat cttgaaacga cccagcaatc gctagataaa
      961 tgcacttttt atcgttcgat ggtagagaga ggaattatta ctgaaacggg tttaccgacc
     1021 caacaggcaa ttgaaaatgg attagtcaaa gattactatg aggaccaagg cttatcattt
     1081 gatgattttt taagaatcta tccaattttt gaagagtatg acgaggagtt atttcaatgt
     1141 attgatggtt attgggagat acctgttgat atgaaagaaa atttagtttc acaattggaa
     1201 tctggcgagt taacttttga agatgcacaa caaattcaag catatcttga agatcgataa
     1261 gtattgaagg aggaagattt atgcgtcaaa atattatttt ggaatgtgtc gaaactggag
     1321 aacgtctgta tctcaccagc aaaaataaac ggaataaccc tgaacggtta gaattaaaaa
     1381 agtattcgcc aaaattgcgc agacgggcaa ttttcaaaga agtgaaatag ctaagtggta
     1441 aaaaggtgac aaaatgggca taccagttac aatcgtttct gggtttttag gggctggaaa
     1501 aacaacgcta atcaatcaag ccttgcaagt ctcaccctac cccagggaag aaattattat
     1561 aattgaaaat gagtttggtg aagttgggat tgatcacaaa ttattagttc atagcgagga
     1621 acgggtgtta caactaaata atggatgtat gtgttgcagt cttcgcagtg atttattagc
     1681 aatattgatt tctttactgg aaacaataga agaacatcaa caaccaattt cacaaatcat
     1741 cattgaaact acaggtatag cagatcccca accaatcatt caaacacttt taacagcgcc
     1801 tcaactaaga ggaagagtat atatcgatag tttattgact gttattgata gtgacagttt
     1861 tgaacttatt gaaagagaac cacaagccct taagcaactt gtcatagcag atcgcatttt
     1921 tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa aaattacaga gagaaatcaa
     1981 aagtattaac cctttatccg atattcgaat gttcacacat agtgaaacga ttcaaaaaaa
     2041 ggatttttat aatttggaaa aattcaatca tcccttagtt tttgaaagtg attcatcaga
     2101 aaatcactat aatggcaaac actatcatca acatcatcaa gaacatgagt ttaaatcaat
     2161 tctcttaact tcagatcaag acataaaaaa agatttttta ttccaattaa ttgaccggct
     2221 gattttaacc aaccaaggga gcctgtatcg ttttaaagga ttgattgctt tgcaagatca
     2281 agattttata gttgccatac aaggggtaaa cgaacaggtt aattttcaat acaccactcg
     2341 acaaaaagac caaaccacta ttattctaat tggaaaaatg ttggatgaag aaaggatcaa
     2401 acagagtttt gataaattac tggcggaaag caaataatga taagtattga ttaggggtaa
     2461 tttctaatgg agaaaacgga tatatctagt gcatatcgac gacttaaaag cccgaatatc
     2521 aaaactagaa aaagggcatt aaaaattatc aaagaacata aacaaaataa aatgaagaaa
     2581 ttagcataat taaggaagga aaaagaatga agaaaatcag tgtaggactt attgggatag
     2641 cagctctagg attattggga gcttgctcca gcactaatga tgcaaaagta tcgaatgata
     2701 aagatggaaa attagaaatt gtaacaacat tctatccaat gtatgatttc accaaaaaca
     2761 tcgtcggcga tgaagcgaac gtggatttaa tggttccagc gggatcagaa cctcatgact
     2821 atgagccctc agctaaggat atggcaaaag ctcatgatgc tgatgttttt gtatatcata
     2881 acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag ttggaagaaa gctgggccta
     2941 atgtagtaga aggaacaaaa gacatgattt tattgccagg tagtgaagaa gaagatcatg
     3001 atcacggaga agaagatcac catcatgaac tagatcctca tacgtgggtt tcacccaaaa
     3061 tggcaattaa ggaagtttca aatatcaagg atcagttagt taaattgtat ccgaaaaaag
     3121 caaaagtatt tgagacaaat gctgaaaagt atttgactaa actgaaacgg ttggatgctg
     3181 actataccac tagtcttaaa gaagcgaaac aaaaaagttt tgtgacacaa catgcagcct
     3241 ttggatattt agctttggat tatggattga tacaagttcc tattgctgga ctaagtcctg
     3301 aagaagagcc atcttctggt cgattagctg aattaaaaga gtatgttaaa aaaaataaga
     3361 tcaattatat ttactttgaa aagaatgcta atgataaaat cgcaaaaact ttggccaatg
     3421 aagctgggat aaaattggaa gtattgaatc ctcttgaaag tcttacaaaa gaacaaatgg
     3481 ataatggtga agattatgtt tcagtaatgg aagacaatct aaaagcgtta gagaaaacaa
     3541 ctatggttgc tggtgaagag gtagttccag aaaaagaagc gaaggatgaa aagacagttg
     3601 ctaatgggta ctttaaagat gccgatgtga aggatcgcga attgagtgat tatacaggcg
     3661 aatggcaatc tgtttatccg ttgttaaaag atggaatttt ggatgaagtt ttcgattaca
     3721 aagcaaaact caataaagac atgacagctg ccgagtacaa agattattat actactggct
     3781 ataagacaga tattgatact atcaacatta aagacaacac cattgatttt gtagtcaacg
     3841 gtgaacatca tcaatacaca tacaaatatg ttggatacaa aattttgaat tatgaaaaag
     3901 gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc aggtgctggt cgttttaaat
     3961 atattcaatt tagcgatcat ggaattgcac caagtaaggc agagcatttc catattttct
     4021 ttggtggaga aagtcaagaa aaactttata atgaaatgca taattggcct actttctatc
     4081 cagcttcatt atctgagcat gagattgctc aagaaatgat ggcccattaa aatccaatct
     4141 ttggaggaaa tacatggtaa gaaaagtatt attgtctatg gattccaaga atattgatgc
     4201 ttatcggcgt tatcaatttg caaagcaaat gagattaaat gggagtacat tagctgatat
     4261 tgctgcaact ttacatgtta gtattgacac agttcataga gtattaaaac gggatcctga
     4321 ttgttgtttt atcaaggtca atactggaag aagtagacgt ttagcaaaac ggcataatag
     4381 cttggccaat taattgataa taccagagta gatcattttt cccttatttg gatttgatct
     4441 gctttttatt atttcttgaa ttgtcaaatc aagcgcaaca ctttatcaaa gaagacttca
     4501 taaggtgttc tccaatctaa acactttcta ggtctgttat tcagtttttc aatcatctga
     4561 tttatcgttg agttatctac cgtagctatc tctttcgatt ttggcaagta ttctcgaatg
     4621 agtctattcg tattttaatt tgttcattgt tgccaagggc atgaggatct ggaaaataaa
     4681 agggcatatt attcaatact tctgtaactg cgaaatgatt ggagaattct tttcctctat
     4741 ctggagtgat cgttttgacc gcctcatttg gaatactgct taaaagttca atcattttct
     4801 cgctcacata agcggctttc tttctgggga ccttctttcc tgctaaaagg aagcatgatt
     4861 ttctgtctgt caaagtcact aaacaagtac cactagtttt gccagctaca atatcggctt
     4921 tccaatgacc aaaatcactt ctatcatctg cggattttgg atggccatgg atcgaatgag
     4981 taaggcaaat cttcccacga gactcgacat gattttttgt atgtcttgtt tttcctcgat
     5041 ggtgcaatga acggatcagg cctttattgc cgtgtgagag gggttctgta tcgaaaagat
     5101 cattgcaaat tgcacggtag atcgtgataa aactaattga aaagtccata tgttcatgct
     5161 tcaaacgatt cgagattttc tctggcgacc actgaaaatc gacaaataaa tgacgaacaa
     5221 ttgaccaaac tcacggatca tttagaatgt gataacgtcc gcattttttt cttcgataag
     5281 cataattttt ttccgctttg gaaggtgatt agtttttttc ttgtttcttc tcagttcatg
     5341 taaaatagta aacgggctat gctgaatgtc gttgccaatt atactaatag aaaatccttc
     5401 ttcgtgaagc agaaagattc tttctcgttt gagtatagta agatgacaat actggctcat
     5461 aattattttc cttctaggag tgttctcgac aaacttatac tagttggata tcgattatga
     5521 ctcattttta tgtgttgcac ttaaagtgta aattcaaggt atagaaagag gttccctatg
     5581 aaaaaatacc tattacaatc ttaactggct atcttggttc aggaaaaacg atacttctaa
     5641 atcatattct gtacggtgat cacggaaagg aaattgctgt cattgtaaat gacatagggg
     5701 aagtaaatgt tgatggggta ttagtcgaca agaaaggttt taaaagaact ggagaaaaac
     5761 ttgtttcgat gccgaatgga tgcatttgtt gtacattacg cgaagatttg attgttgaac
     5821 tagatcaatt agtgcaattt tctgacttga atcaaattgt tattgaagct tcaggaatca
     5881 gcaaaccaat tccaattatt caagcaatta tattagctaa aaacccttta gacattgatt
     5941 tgacccaacg attagagatt ggtgccatta ttatagtagt ggatgtaaaa cgtttttggg
     6001 ataatttcga aacgggtgaa aagattcagg aaagaaaagc tgaaggtaat gtagttgaag
     6061 aagaacagga tattcgtggc ttgttaattg atcaattcga gtgttgtaac atattacttt
     6121 taaataatag tgacttagtg tcaaccaaga cgatttacaa aattaacggt ctaattgcta
     6181 agttacagtc caatgccaaa ataattagga caattaaagg tgaagtagac ttatcgaaaa
     6241 ttctaaatac aaatcttttt aatatagatg agacttccta ttcaatgggc tggttcaaag
     6301 agttagagct tggctatcaa aatcatattc ctgaaacaga agaatatggt atctcaagtt
     6361 ttatctatcg aaatcggtta ctccttgacg cacaaaaatt tgcccatctt acggataaaa
     6421 attttcctgg caatattaca agagcaaaag ggtttctttg gtttgcccaa catagtgatt
     6481 tttcgacgat ctttgaaaca tcgggtagcc ataaaagtat tgaatcatta aattattggg
     6541 tagctagttt acctaaaatt gagtgtcaaa tgattataca aaaaaacccg aattgaagga
     6601 agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg gtatgaagat
     6661 ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc ttaatgcaga
     6721 taatttccga aaaatttcac catttccatg gaaaaattct aatatgttta gccagatatc
     6781 gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa ttattggcat
     6841 tttgattatt ccctctttta ttcccttatt tgtattctct atagaaaagg agaatgaaac
     6901 aactgaagat gctatcaaat atgccatcgt tcaaattgat ggcaaggaaa tggatcgctt
     6961 cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg gacaatataa
     7021 tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc ctgatcaaat
     7081 cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt taccccataa
     7141 gttagttatt agtattgaga aaaaagaaag caaagattac tacatttact aatctggaaa
     7201 tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca ggggctttat
     7261 taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc acaagtatct
     7321 ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttattt tggctcttcg
     7381 tcaatgagtg ttggtgaaga aattcaatag aataatcgag atactcacac ggctagacag
     7441 ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag ttgggtagtg ttcttagttt
     7501 tcttgaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga aagtttctaa
     7561 agccataggc aattcgtttt agtactttga tatggttgtt caaacattct atagggccgt
     7621 tggaataggg aactcgcaac gctcgtttga tttctgttcg atatttcttg aaggtcgtga
     7681 acactggttg atagtgctct ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt
     7741 caggaacatc ttgtcttctg atagcggaaa gaaaaacctg gtagacttcg tagcctcgct
     7801 ttaaggagtc atcataatat aatagccgat cgactatctc cgcttctgtc agatgatctc
     7861 gaaatgacgg acgccagtag cgatgctgac cattcaattc ccacgcattc ttttgaagta
     7921 gtctccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag gtcttcatta
     7981 cttgaattcg gtggttggta aaggcccgac cgatgtgttg gctaatatga aagcgatcgg
     8041 cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt gtatagaaat
     8101 ctgaaaccac tagacgtact tgttcacgga cttttctagg gtagcgcaag aaatagtcgc
     8161 gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca
     8221 tcatgacaaa gctcatggct cccgagacgt ttttcaccga tttgaattca tcgaaacaca
     8281 ccacttctgg taacggctgg ttcaaagaaa cggttttagg ccgaaggctg cgaagtattc
     8341 gaatgacagt ggtcggagaa atgtgcttca tacgtgcaat caaagacata gaaataggct
     8401 cacttaatag ctcaagaatg gcttgtttga ctctgcgggc aatggagcag cgacgatcgc
     8461 taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag cgctgtttgt
     8521 taatccgcac aatggtcttg tagttactga catcattcaa taagatggat acttttttcc
     8581 agccccattt aatgatcgtt tgcttatttt gatagtggca atggaagcat tcctctggtt
     8641 gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attaatgcgc tctttgcgaa
     8701 aggcatcttc atgaaaggta agatttaggt ctagaatatc tagtaactct ttggtaaact
     8761 ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta ttctaaagga
     8821 tttcaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt
     8881 caccaacact agatattata gatcccttat tttcttcgct tcaaattgtc aaggagtcat
     8941 cataaatttt gtatggattc ttttgtttgg gtagtagtct tctaaataat cttggattaa
     9001 caccttcatc tctattttac ttttatattt gttggagtcg attaattctc atttgattga
     9061 tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc ccagtttggc tcattgaatg
     9121 tgtgatgtgg tggtcggtca acagctcttg ataaataaaa gaccgatgct ggctacctct
     9181 atcggaatga attaccgtag gcaattctcc ttaataaatt tgtaatggac ggacgacaat
     9241 ctcttttttc atgtgtgaat ccaccatata gtaacgtaga atacgagttg ctaagtcaat
     9301 ataggtggac aaatagaggc gaccgtcatt acatggaatg taagtaatat ccgtcaccca
     9361 aacagcatca atttggttct gttcaaatta tcgattcaac atttttttta ccatttcttt
     9421 gattgttttt tctctaccat gagaagattt gcgtcgatga aatcctcttg tgcataaatt
     9481 catttcacgc ataagaacag ctacaacacg tttattgatt tcgattcctt catcgtaaag
     9541 tttttgagtg atccctggag atccgtatcg atctttgtgt tcgtagaaca ctcgtcttcc
     9601 tcttttagtc gtttattttc ctgttcaaga atgtagaaat ctgctttatc aggtaatctt
     9661 tttccttgtc cagcaaaagc atttttcccg tatcgacgat attctgaaac ccatcgggaa
     9721 attgttggac gagagatatt gtattcttca gcaattttct tggtagttac ttttttctca
     9781 aggatatcct gtgaaatctt tagtttaaat ttacgatcat atgttttttt caatagagtt
     9841 cattccttcc ggtaacgtaa ctgatagtca agaaaagtgt atcatagccc aacttagtat
     9901 cagggtttgt caactaaact gtggaagttg atagataaga gtttttaaca accaaaattc
     9961 tcttgactat ttttgaaatc agacaaattt tgattggaca catagttgaa taaatctacc
    10021 gtcgttacgg aaggtaaatc gcatgctttt caattctaga ggagaagtcc tcgttgaagt
    10081 accatggccc agttctggat tcgacctcct gcccacttcg tacgtaaata aagtaatttt
    10141 aggagtgaga ctgtgacaaa agttatttta agctaaaaac gaattaaaaa acgttaataa
    10201 ttaaaatcat t
//
LOCUS       DAYYBG010000094        10137 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000094 DAYYBG010000000
VERSION     DAYYBG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.095"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHAUN_002323"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHAUN_002323"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFM8460996.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHAUN_002324"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHAUN_002324"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFM8460997.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHAUN_002325"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHAUN_002325"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFM8460998.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACHAUN_002326"
     CDS             complement(4028..5044)
                     /locus_tag="ACHAUN_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8460999.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5298)
                     /locus_tag="ACHAUN_002327"
     CDS             complement(5146..5298)
                     /locus_tag="ACHAUN_002327"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461000.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="ACHAUN_002328"
     CDS             complement(5315..6634)
                     /locus_tag="ACHAUN_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFM8461001.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7012..7104)
                     /locus_tag="ACHAUN_002329"
     CDS             complement(7012..7104)
                     /locus_tag="ACHAUN_002329"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8461002.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
     gene            complement(7375..8355)
                     /locus_tag="ACHAUN_002330"
     CDS             complement(7375..8355)
                     /locus_tag="ACHAUN_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8461003.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="ACHAUN_002331"
     CDS             complement(8368..9246)
                     /locus_tag="ACHAUN_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFM8461004.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="ACHAUN_002332"
     CDS             complement(9262..10062)
                     /locus_tag="ACHAUN_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFM8461005.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
BASE COUNT         2734 a         2352 c         1718 g         3333 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac actctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtag ctactacggt aacaactata aattcttttt gattatctgt caagagt
//
LOCUS       DAYYBG010000095         9968 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000095 DAYYBG010000000
VERSION     DAYYBG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.096"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..733
                     /locus_tag="ACHAUN_002333"
     CDS             254..733
                     /locus_tag="ACHAUN_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8461006.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACHAUN_002334"
     CDS             766..2994
                     /locus_tag="ACHAUN_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8461007.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACHAUN_002335"
     CDS             3123..4766
                     /locus_tag="ACHAUN_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8461008.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACHAUN_002336"
     CDS             complement(4839..5384)
                     /locus_tag="ACHAUN_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8461009.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACHAUN_002337"
     CDS             complement(5407..5892)
                     /locus_tag="ACHAUN_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8461010.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACHAUN_002338"
     CDS             complement(5882..6694)
                     /locus_tag="ACHAUN_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8461011.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACHAUN_002339"
     CDS             complement(6833..8365)
                     /locus_tag="ACHAUN_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8461012.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="ACHAUN_002340"
     CDS             complement(8474..9505)
                     /locus_tag="ACHAUN_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8461013.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3322 a         1844 c         1916 g         2886 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg
     9841 tctaacgata ttgttgggat aattcttaaa gaagtattag aaatagttgg tacttcatta
     9901 ctttacatga agatttattc tatggtagga gcaattatat tgagaattaa tcctttttta
     9961 tataagtt
//
LOCUS       DAYYBG010000096         9930 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000096 DAYYBG010000000
VERSION     DAYYBG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.097"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1186
                     /locus_tag="ACHAUN_002341"
     CDS             <1..1186
                     /locus_tag="ACHAUN_002341"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8461014.1"
                     /translation="KAFGLFLLTINRNVYSKGIEIMWLEKINDKKFKYTERYTDPLTE
                     KKRKVSVTLSSNSRQAWNQANLLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVK
                     ESSYRTTQVYLSLISKYIKDDVLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTG
                     MIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQ
                     SRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTI
                     ELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKK
                     VSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKI
                     EI"
     gene            complement(1268..3040)
                     /locus_tag="ACHAUN_002342"
     CDS             complement(1268..3040)
                     /locus_tag="ACHAUN_002342"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8461015.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3043..4755)
                     /locus_tag="ACHAUN_002343"
     CDS             complement(3043..4755)
                     /locus_tag="ACHAUN_002343"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8461016.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4870..5538
                     /locus_tag="ACHAUN_002344"
     CDS             4870..5538
                     /locus_tag="ACHAUN_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8461017.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5605..6393)
                     /locus_tag="ACHAUN_002345"
     CDS             complement(5605..6393)
                     /locus_tag="ACHAUN_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8461018.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6476..7948)
                     /locus_tag="ACHAUN_002346"
     CDS             complement(6476..7948)
                     /locus_tag="ACHAUN_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8461019.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8078..9271)
                     /gene="metK"
                     /locus_tag="ACHAUN_002347"
     CDS             complement(8078..9271)
                     /gene="metK"
                     /locus_tag="ACHAUN_002347"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8461020.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(9269..9348)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(9537..9608)
                     /locus_tag="ACHAUN_002348"
     tRNA            complement(9537..9608)
                     /locus_tag="ACHAUN_002348"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9573..9575),aa:Glu,seq:ttc)
     gene            complement(9620..9693)
                     /locus_tag="ACHAUN_002349"
     tRNA            complement(9620..9693)
                     /locus_tag="ACHAUN_002349"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9657..9659),aa:Ile,seq:gat)
     gene            complement(9706..9776)
                     /locus_tag="ACHAUN_002350"
     tRNA            complement(9706..9776)
                     /locus_tag="ACHAUN_002350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9742..9744),aa:Gly,seq:tcc)
     gene            complement(9781..9853)
                     /locus_tag="ACHAUN_002351"
     tRNA            complement(9781..9853)
                     /locus_tag="ACHAUN_002351"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9818..9820),aa:Phe,seq:gaa)
     gene            complement(9857..9929)
                     /locus_tag="ACHAUN_002352"
     tRNA            complement(9857..9929)
                     /locus_tag="ACHAUN_002352"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9894..9896),aa:Asp,seq:gtc)
BASE COUNT         3193 a         2109 c         1650 g         2978 t
ORIGIN      
        1 aaaagccttc gggctttttc ttttaacaat taataggaac gtatattcga aaggaataga
       61 aataatgtgg ttagaaaaaa taaatgacaa aaaatttaag tacaccgaac gttatacgga
      121 tcccttaact gaaaaaaaga gaaaagtgtc cgttacttta tctagcaatt cacgccaagc
      181 ttggaatcaa gctaacttac ttttgaatga aaaaatcgca gaaaaaataa aacgaaatga
      241 agaattacca ctaacttttg gagaattaaa aactaaatgg gatgaaaaat acaagccaac
      301 tgtaaaagaa agttcctatc gaacaactca ggtttatcta tcattaattt caaaatatat
      361 aaaagatgat gtacttgtaa aaaacgttaa tagtaatctt atacaagata tgctagatta
      421 ctactatttt gaaaaaaatt tatcatataa ttatgtaata catttaaaaa cttttacagg
      481 aatgattttt aaattcgcca accgtcgata tggattaaaa tataatcccg taaatggggt
      541 ttctttagca agaaaaccta agacaaacga agaaataaaa aaagaaaaag atggttattt
      601 atcaaaagaa gaagttcaaa tgattgctag taaacaaaaa agttcacatc aacagtcacg
      661 ttattcttta attactaaat ttttgttttt aactggacta cgttatggtg aattgatatc
      721 tttgactgaa tctgattatg atggtaataa aattacggtt tctggtactt atgattacga
      781 gttaaaaatt aaaacaacaa ctaaaaatac aggttcttat agaaccattg aattatcaag
      841 taatgctaaa gaaatcatag atcaattaat tgaagaaaac aaattaatta agaataataa
      901 agataaatac attttcatct ctaaaaatgg taacccgata tctattcaag catataatca
      961 atctttaaga gcagtatcca aagaattaaa cctagataaa aaagtttctt ctcacatgtt
     1021 aaggcattcc cacatttctc tacttacaga attaggaatc ccattaaaag ccattatgga
     1081 tcgtgtagga catgaagatt caaaaacaac attaaagatt tatactcata caactaaaaa
     1141 tatgcaaaat caactagtag aaaaactagg aaaaatagaa atttaaattg ccccttttct
     1201 gccccttata aaaacaaaca acataaaata tataaaaaga accccgttat aacgaggttc
     1261 ttttaattta actttcaaat acaaattgat tatgataaag ttccgcataa aatccatttt
     1321 ctgctaataa ctcgtgatga ctgccttctt caatgacttc cccatcacgc aagacaacga
     1381 ttctatctgc attcaagatt gttttcagcc ggtgagcaat cacaaagctg gttcttcctt
     1441 tgattgcttc atccattgct ttttggatcc ttgcttctgt taccgtgtct acattacttg
     1501 ttgcttcatc caaaatcaat aaagatggat ttgtgatgat cgttcgagca atactgatca
     1561 actgcttttg acctgtactg aataagttat tttcttcgga tatctctgta tcataacctt
     1621 tttctaacgt catgatgaag tcatgaatat tcgcttgttt tgctgcattg atgacttctt
     1681 catctgaagc gtccggtttg ccgaatgtga tattgtcttt gatcgttccg gagaacaata
     1741 cagattcttg caaaacgatt cctacatgtc tacgtaatgc atcaagatca taatctcgga
     1801 tatctattcc atcgatgact actgaaccat cattgacatc gtaaaaacgg tttagcagat
     1861 tcatgatcgt cgttttcccc gatcctgttg gaccaacaag agcaaccatt tctcctttgt
     1921 ccacattgat cgagacatct ttcaagacta gtttttctgg agaataacca aaatctacgt
     1981 gctgcaacgc aagacctttt tcaacaccat caaatgcttt acagttttct ggtttgactt
     2041 catctttttc atcaaatatt tcattcaatc gtcttgcccc tgtaatcgct aattggatca
     2101 tgctgtatcc agatgaaatc tgcatcagtg gttggtaata ttgttgtgaa tactggacga
     2161 atgtcacaac taagcctaat gccaccgaac gttccaaatc accatttaat gctaaccagc
     2221 cgccgaaaaa gataactatc gctgtattga ccagtgacat tccttgcatc atcggaaata
     2281 gtagaccaga ataagcttgt cctttgaagg ttgcttcgcg aacactttga ttatgctcta
     2341 agaatccttc aatcgtttct tcttgtaagc catttgtgat gatgacacgt tgtccgctga
     2401 ttttttcatc catataacca tttagtttcc ccacttcatc ttgctgaaga tccacatatt
     2461 tccgtgcttt gcggataatc aagacagcta tcaaaacggc tactggagtg gaagcaattg
     2521 tagcccaagc taactctaca ttttggcgga acatcatgat caagatcccg acaagtaaaa
     2581 cagcattcgt gatgacttga agcaaagctt ggttcaaact attttgaata ttatctaagt
     2641 cactggtaaa acgacttaaa atttcaccgt cttgatgaga atcaaagaaa cggatcgtca
     2701 atttttccaa tttgttgaat agaccgatac gcattcggtt cgttgacttc cctactactt
     2761 gtgtgaacaa aatactatag ataaagttgg ctgcactagc tagtacataa aacagcaaga
     2821 gtttccatat tacagaaaca aatttgcttt tatcgtctac gccttgcatc aagcctccta
     2881 cgtaattagc taattcttga aaagcttccc cgatgtattg aggtgccttt acttgtagat
     2941 aggtagcaat gatcactgtg ataaagataa agaaaaagga gagtttgtac cgcttcaaat
     3001 agtgatagaa aaatttactt gcttttatta gatcagtcat acttactcct ccgttccttt
     3061 ttgagtttca aagatttcct gatagacacg gtttccagct accaattcct catgggttcc
     3121 ttcgccgact aatctgcctt catccaatac taagatgcgg tctgctttga caacagaagc
     3181 aattttttgt gcgatgacaa tcgtcgttgt tcccttcaaa tctcgatcga gtgcttctcg
     3241 gaccagcttt tcagagcgag catccagtgc actcgtactg tcatctagga tcaagatctt
     3301 tggattaccg ataacgccac gagtaatgga taaacgttgc ttttgaccac cagaaaagtt
     3361 actgctacgt tcttctattg gtgcttcgta acgatcagat aacttttcga tgaattcttt
     3421 tgcttgagca atagaagaag catgttccat ctctttttct gttgcattct ttttcccttg
     3481 gcgcaagttt tgagcaatcg ttcctgagaa taaaattgct ttttgcaaaa caaaggaaac
     3541 cgtttgtctt aaactctttt catttacttg acgaagatct acaccaccta cttcaacttt
     3601 gccttctgtc ggatcaaata gacgagggat caattgtgct agtgttgact tcccggcacc
     3661 agtcgccccg acgataccaa ccatttctcc aggtttgata gaaaagctga tatctttaag
     3721 cgtatctgta tcctcaccag gataacgaaa agaaacatgg tcaaaagtga cagaacccgt
     3781 caactcttgt tctggaacgt ctaaataagt aagatctggt tcagcatcta aaatttccgc
     3841 aatccgcttc agcgatacgg ctgctcttga agtcatcatc atcatcattc cgccaataat
     3901 gattgccatc ataatctgca tcaagtagtt catgaatgaa gcaattccac caattaacgt
     3961 aggatcatct ttaacaagat cgctcacaaa aaagatcgcc ccaaccacag ccaaattggc
     4021 agccagcatg aaggacggga tcatcacaga aaataatgta ccgactatga tgttgtgttt
     4081 tgttaagtcc tcacttactt tgctgaatga cttcaattgg ttttcttctt gtacaaaaga
     4141 tttcactaca cgaatcccta gtaagttttc ttttgcaatg ctattcacac gatcaatcag
     4201 tttttgaatg atcatgaagt gcttgcccat ccttgtaaat gaaagtgccg taatcagaaa
     4261 taccgtaacg ataagcgcta cgatgatcca ccataattgc ggcatcgtga tcatagctaa
     4321 aatgaaggca ccaataaata agaatggaat tcgaaaaagt gattgcaaag aaatcatcac
     4381 taaattttgg atttgcgtga tatcatttgt tagacgaaca acaagatttc cagcagagaa
     4441 ctgttcgata tttccaaatg aaaatgtctg gatctttcga aatgcttctt ctcggatatc
     4501 tgcacttacg ccttgagcta cttttgcaga gaaaatcgtg ttcgttacac ctgcggctaa
     4561 tcctagcaaa gccagactga tcaggtaaat accaattgtt ttcatttcat cattatcttc
     4621 tgtaataata gcttccaata cttgctgtag taacttaggt tgccataatg ctgctgcaac
     4681 catgactgct acggacagca aggaaataaa aacagctact ttatattttt tcgcatgtcg
     4741 taagattaat ttcattcgtt gattacaccc ctttgtttaa attgacagga ctaatttaac
     4801 actgtaaaat tagaccgact aacaatttac catgtttaaa aagataatgc aatacacgag
     4861 gttgataaaa tggaaataaa gctttctcaa gcaatcatca ttcaggccgc ctttgatatc
     4921 ctggctgaaa cacaagctct ttcgcagctt tccatgcgaa acatcgcaaa cagaattcat
     4981 gttcaagcac ctgctttata ttggtatttc aaaaacaaac aacagctctt gcaaataatg
     5041 gctgaaacaa tggaagaaga attagcgctt ccagatccaa ctttgccttg gaatgaacag
     5101 ttgctgcagt atatggaaaa ttactatgac ctttatacac gtttcccttg tggggcagaa
     5161 ttagaaatca atactgtgcc cgcttttccg agtcgattag aacatttgga gcaaatgatc
     5221 caattacttg ttcgtgaagg attttcagtc acccaaagtc atcaggccat tgcctctctc
     5281 cacaatttat tgattggaca gttaatggat cagcagcatg aacagcagct tcgacaaaaa
     5341 attatggacg gaaatgaatt tttgaaagaa acagttctat ttatgcgtaa ttatgtagtt
     5401 gaaaatcaat tagagggatt taaagaaagt atgcagtccc atgcagaatt gaatgaaaaa
     5461 gcactctttc taacgagtgc acaaacctat attgaaggct tatgccaaca aaataaaaga
     5521 agagaaaacg agcagtaaaa aagctcctat agagatcact atgtttgtga tctctatagg
     5581 agctatcttt ttaagactct ttttttattt ttcttccatt agccacgcca atgcacgttg
     5641 aatccattgg tcccagaaga tccagttatg atcgccaggt ccttgttcaa aggtgaccgc
     5701 caagccttgt tcctgcatag cagataccat ttcctgattt gcctgataaa gaaaatcaga
     5761 ttccccacaa gccatataaa atcttggaac tggttttccg ctttttacta gttctttcaa
     5821 taagtattgc ggatcatttt ttgaaccttc gatttgatcc aatggtccaa agatcccttc
     5881 ccaaaaagat gctttacgaa ccatcaaaag atcatccatt cgatttccta cagaaacagc
     5941 gcctgaaaga gagattactg ctgcgaaatt ttctgatttt gcaaggccta gttttaacgc
     6001 gccatatccg cccatcgata atccagcggc atatgttttt tctctttttc ttgttaattg
     6061 cgggaataat tcatgacaaa tctcaggtag ctcttctgca atgaatgtcc aatacttcat
     6121 gtcataaact gtatctgtgt accagccaag atcagtagat ggcatgatga cagctaatcc
     6181 gtagtttgct acataacgtt caatcgacgt acgtctttcc catacactat gattgccacc
     6241 cattccatga agtaaatata aaacaggtat atccgttgtc tcttgctctg aactggtgcc
     6301 aatcaccttt tttgttgttt gcggtagtat cacatttaga ttgacttcca tttctaacac
     6361 attcgaataa atatttgctt gaataaatgc catattttcg ccttccttag taagaagtta
     6421 tttttgaata actttaaatt tgaatgattc ggctacgtac atatcttaac cgaaactact
     6481 gttttatttt accacttttt tcatgaacga aaagcgttaa gaccaaagca atgatcacga
     6541 gtaacaagcc aactagataa acggaatgta acccatcata aagaattttt cttaatggta
     6601 acaaaatatc cgcatcaata tatttagccg tttgtggatt caccagttta ttcatgatat
     6661 ctgcatcatt tgttaaattt gctgctgcaa gttctcgttt agttacccca tttatcaata
     6721 aaccaaaaat agaaaccatg actgtttgac caattgttcg aactaacgta ttaaaagaag
     6781 ttgctacacc aagctggtca cgagatacac tattttgtgc actgacagtc gaggtagtca
     6841 tggtcacacc taagcctata ccgatgaccg ttgatatagc aaaaaaccat aaatagttac
     6901 ttctgaaagg cataagatac aaccaagcgc ccccaatagc tgcgattagt agtccaattc
     6961 tcaagaccca atgagttttc atttgagtca tccacttgct tgcaaggaaa gaaccgacca
     7021 tccagactag tgataatggt gctaatacca gcccaccaat tgctgcattc agccctaata
     7081 caccttgcat ccacataggg atataaacat cgattcccat caaaaaacca ctagccaatg
     7141 ctgcagccaa gttgaccaca acaaatagtg gatttttgaa taaatgcatc atgatgacag
     7201 gatctttagc tcttttttcg gcaaaaacaa aaatacctaa cattacgata ctacctagca
     7261 gcaaagcaga aacaagcgga ctcattcctt cgtctcctaa taattggaac cctaacagca
     7321 atgagacaag catggccatc aaactaaaac taccaaaaat atccattggt ttagactcat
     7381 gtatgatttt tggttcattt aaatatagcc aaatcaaaat aattaataga attccgatcg
     7441 gcacattgat aaaaaagatc cagtgccagc tgatcgtttc aacgatgaat cctcccgcaa
     7501 gcggtccaaa aacactggca atcccccagg ctgaactatt caaacctaaa acttttgctc
     7561 gcttttcaat agaataaaga tcgccgatga tcgtcaaagc tactggcatc attgccccag
     7621 cacccatccc ttgaatcgca cgagcagcaa tcaacccaat catgctttga gataaaccgc
     7681 aaaataacga accgataata aacaataatg tcccaaaaat aaagattggt ttccgtccca
     7741 ctttatccgc aagttttccg taaatcggag taagcatagc gtttgtcagc aaataaatcg
     7801 aaaataccca gttcatgatt tccattccat gtaatgaacc gacaatcgtt ggcatagcgg
     7861 ttgttacgat tgtaccttca atagccgtca tgaacgttgc aatgaaaata ctcactgtga
     7921 ccaaagtaac atttgtttct tttttcatct tattcctccc tttcattatg acaggatcta
     7981 aaaatagata aataattact atctttttca agaggttaac tgccataaaa aaagctggaa
     8041 caaccaccac ttggcgatcg ttccagctga atataactta ttttgctaaa cttgctttca
     8101 acgcatctac tttgtccgta tgttcccaag gcaaatctac atctgtacga ccaaaatgac
     8161 cgtaagcagc tgtttgtttg taaatcggac gacgcaaatc aagcatctcg atgattcctg
     8221 ctggccgaag atcgaagttt tctctcactg cagcaatcag ttcttcttcc ggaaccgttc
     8281 cagtaccgaa agtattgatt gagatcgaaa caggttgagc aacaccaatc gcataagcta
     8341 gctgtacttc tgctttgcga gcaagccctg ctgcaacaat gtttttagca atataacgtg
     8401 cagcatagct ggcagaacgg tcaacttttg tcgcatcttt accagaaaaa gcaccgccac
     8461 catgacgagc ataaccgcca tacgtatcaa caatgatttt tcttcctgtt aatccggcat
     8521 ccccttgagg accaccaatg acaaatcggc cagtcggatt gatataatat ttcgtttgat
     8581 catctaataa ttcagctgga ataacttcct tcactacttt ttcgatgaca tcatgacgaa
     8641 ttgtttcatt atctactgca tcatcatgct gtgttgaaat aacgattgta tctacgcgtt
     8701 ccggttgtcc ttgatcatca tattcaaccg ttacttgaga ttttgcatct ggacgtaaat
     8761 acgttaattc atttgattta cgcaaatctg ccaagcgtcg taccagacga tgactcaaag
     8821 cgattggtaa aggcatcaat tctggtgttt catctacagc aaacccaaac attaaacctt
     8881 ggtcaccagc accaatctca tctaatacgt cttttttatc ttcatcccgg atctcaagtg
     8941 cttcatcaac tccttgagca atatcaggag attgttcatc aatcgcaacc aatacagctg
     9001 ctgtatctcc atcaaatccg aattttgcac gtgtataacc aatttctttt attgtttccc
     9061 gtacgacttt ttgaatatct acatatgccg tcgtagatat ttctccaaat actaaaacaa
     9121 gaccagttgt tacagacgtt tcacacgcta ctcgtgctgt tggatcttgt tttaaaattg
     9181 catccaagat tgcatcactg atttggtcag caattttatc tggatgtcct tcagaaacag
     9241 attctgatgt aaataagtgt ctttctacca ttataatgat tccccctagt taaattcggt
     9301 tacaaggcat ctccgtcctg cggatccttt cgggaacttg caacgaacca agtatagcag
     9361 ataaaacagt tttctgctcg tattttttca tttttttgct taaaaaatat aaaaatattt
     9421 tacattagaa ctgtccaaaa aagcttagca caatcatcca taaagataac tctgctaagc
     9481 tttttaaaat cattctttca gataaaaaga aaaagatact caaaaagtat cttccatgac
     9541 ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc
     9601 aacgggccaa taaaataaat gggcctaaat ggactcgaac catcgacctc acgcttatca
     9661 ggcgtgcgct ctaaccagct gagctatagg cccataaaaa ctaaaagcgg gtgacgagaa
     9721 tcgaactcgc gacaacagct tggaaggctg tggttttacc actaaactac acccgcataa
     9781 tggcgcggga cagaatcgaa ctgccgacac atggagcttc aatccattgc tctaccaact
     9841 gagctaccga gccaaacggt tccgacggga atcgaacccg cgatctcctg cgtgacaggc
     9901 aggcatgtta acccctacac cacggaacca
//
LOCUS       DAYYBG010000097         9813 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000097 DAYYBG010000000
VERSION     DAYYBG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.098"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACHAUN_002353"
     CDS             complement(34..2154)
                     /locus_tag="ACHAUN_002353"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8461021.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACHAUN_002354"
     CDS             2384..3562
                     /locus_tag="ACHAUN_002354"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8461022.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACHAUN_002355"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACHAUN_002355"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8461023.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACHAUN_002356"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACHAUN_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8461024.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACHAUN_002357"
     CDS             complement(4915..6168)
                     /locus_tag="ACHAUN_002357"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8461025.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACHAUN_002358"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACHAUN_002358"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8461026.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACHAUN_002359"
     CDS             7746..9092
                     /locus_tag="ACHAUN_002359"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8461027.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACHAUN_002360"
     CDS             complement(9400..9786)
                     /locus_tag="ACHAUN_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8461028.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3129 a         1782 c         2009 g         2893 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta tta
//
LOCUS       DAYYBG010000098         9770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000098 DAYYBG010000000
VERSION     DAYYBG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.099"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..1435
                     /gene="rlmD"
                     /locus_tag="ACHAUN_002361"
     CDS             62..1435
                     /gene="rlmD"
                     /locus_tag="ACHAUN_002361"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8461029.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            1438..1566
                     /locus_tag="ACHAUN_002362"
     CDS             1438..1566
                     /locus_tag="ACHAUN_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8461030.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            2006..2194
                     /locus_tag="ACHAUN_002363"
     CDS             2006..2194
                     /locus_tag="ACHAUN_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8461031.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            2272..3285
                     /locus_tag="ACHAUN_002364"
     CDS             2272..3285
                     /locus_tag="ACHAUN_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8461032.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(3352..4386)
                     /locus_tag="ACHAUN_002365"
     CDS             complement(3352..4386)
                     /locus_tag="ACHAUN_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8461033.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            4501..6726
                     /locus_tag="ACHAUN_002366"
     CDS             4501..6726
                     /locus_tag="ACHAUN_002366"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8461034.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            6744..8627
                     /locus_tag="ACHAUN_002367"
     CDS             6744..8627
                     /locus_tag="ACHAUN_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8461035.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            8608..9582
                     /locus_tag="ACHAUN_002368"
     CDS             8608..9582
                     /locus_tag="ACHAUN_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8461036.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         3251 a         1748 c         2041 g         2730 t
ORIGIN      
        1 taattagctg atttatagta gagttcagta ggattgaaaa ataggcttaa aggagcaaaa
       61 tatgacagaa cagcaaatag taaagaaaaa cgaacgatat acagtcgaaa tcgtcgactt
      121 gagttatgaa ggaatggggg tagcaaaaat cgatggttat ccattattta tcgaaaatgc
      181 actccctgga gaagaagtag aaatactagt agtaaaagca ggcaataagt ttggatatgg
      241 aaaagtcgaa acatttatta cacaatcacc tgatcgtcag cctgtagaaa acaatgcatt
      301 acttcgcata ggtatcgcac ctttagccca tttgaagtat gaacaacagc ttgtcttcaa
      361 acaaaaacaa gtagagcatg taatggcgaa gattgctaag atgccagaag ttcctgtact
      421 tccaacggtc ggaatggcag aaccattcgg ctaccgtaat aaagcacaga tccctgttcg
      481 ccgtatcgat ggacagttag ccaccggttt ttacaaaaag aatagccatg atctagtccc
      541 tatcgaagat ttttatatcc aagatcctgt catcgatcaa gcaatcaaag tagtcagaga
      601 tatcttgcaa cggttccaag tacgaggata taatgaagaa aaaaatgaag gacagcttcg
      661 tcacatcatt atccgtcgtg gtcattattc ccatgaaatg atggtcgttt tagttactcg
      721 taaagaaaaa ttctttaaat ccaaggaaat tgctgaagct attcatgaag aattgccaga
      781 agtggtttcg gtcatccaaa atatcaatga agaaaaaaca aatgtgattt taggagacaa
      841 agaaaaagtt ttatatggca gagactatat cgaagatcag ctattaggaa aaacctatcg
      901 aatctcagca aagtcatttt atcaagtaaa tactcaacaa gcagagtatc tgtaccaaac
      961 agccatcgag tttgccgagt tagataaaaa agacattgtg atcgacgctt attcaggaat
     1021 aggtacgatt ggcttgtctt tagcagacag agtggataag gtgtatggga tggaagttat
     1081 tccagaagca attgaagatg cacagttcaa tgctttaaat aataaaatcg aaaatgttca
     1141 ttatgaagtc ggaaaagcag aaaaagttat gaaaaaatgg caagaaaaag ggatcaaacc
     1201 atctgtgatc gtagttgacc ctccacgaaa aggattagat catcatttta ttgaatcagc
     1261 aatcgaaatg tcaccagaac gaatcgtcta tatctcatgt aatccggcaa cttttgcaag
     1321 agatgctaag ttatttgcag aagcaggtta tgccgtgaag aaagttcagc ctgtagatct
     1381 tttcccgcaa acccatcata tcgaattagt cgcttcattc gttaaaggag aataaaaatg
     1441 gaccaaaaag aaaaaatgaa agaattgatc aaaaagaaac aaggtggagg ccgatccaaa
     1501 caaatggaaa cacctaagaa tgatcgtaaa aacatgagaa aaggccctaa aatctttaat
     1561 aaatagacgt gctaaagaaa gactaagaag tgttaacttt tgttattgtt tcttagtctt
     1621 tttagttagt ctacattatt ttggcgattt gccaataagg actgttgagt tgtgcaaaat
     1681 caaatctggg tcagaatgaa cgtcattctg tctcagattt ttcgttattt caatttgatt
     1741 aactgattga tcaaaaagat ttttattttc atattctcaa atgattcaac actaaatata
     1801 catgtttgac gcggaaatta ttattcgatt ttggcggcat caggggggat gacgacgtag
     1861 cagacccatt attttactca agaaaatgtc cgtgcctatt atttagaata cgatagtgaa
     1921 cgtgctggga gttttgttct tttagagaaa gtctctgaag ataaattagt tgtcttagat
     1981 tttgactgta acgaaaaatg gcgaaatgga agagcaccag caagtgattg accggatcta
     2041 cgaagcgaca cgatatctgc ctttggatcg tttgtgctta agtcctcaat gtggatttgc
     2101 ttcaacagaa gaagaaaatg ttttgtttga agaacaatgg aaaaaatttc ttttgtcaaa
     2161 agcattgcag aagaagtttg gggaaaatca ttaaatcatc taggagagct tgataggaaa
     2221 taacactatg gtataatcag aatgcttaat actaactact gagggagcct tatgaaaaag
     2281 aaaattctta aatttatcgg atggttcttc ggaatcattc ttgggatcgc gtttgcaatc
     2341 tttcttgcct tccagctttc ccccagacct ggagcattct tgatcaacca tatgttttca
     2401 aatactgttc aaatcactga taaaaaaacg ttcgatcaag cagaaaaaaa cgtggcgaaa
     2461 aaaacagatt tgacgtatga gtccaagtat aaagagaata cttatgacat ctactatcca
     2521 aaaaacagca aggggcctgt tcctgttcta ttttgggtac atggtggagg attcgtcggc
     2581 ggagataaat cgggtgtcaa agaatttgcg acaaaattag tttcagacgc caacatcgca
     2641 gtggttgcga tgaattatga attagcgcct gattcagaat accctaatca agttcttcaa
     2701 gtgaatgaac tgatcaaaga tttattagct gaagaaaaag acgacaaatt gctagacatg
     2761 gaacaattat tttttggtgg agatagtgca ggttcacaaa tcgcattgca atatgcagct
     2821 atacaaacga atgatgcgta tgctaaacaa atgaaaatca aacagctact tccaaaagat
     2881 agtctgaaag gaaccatttc ttattgcgga cctgttgatt tgaagctaac agcacagcag
     2941 cattcggatg atcgatttat gaagtttttc gtcaaaaccg ttgcgtggtc gttgattgga
     3001 acaaaagatt ggaaaacgag tgagcaattg aaagaagcta gtgtggctga ccatgtttct
     3061 aaggaatttc cgccaacgta tataactgac ggaaatgcat actcattcca ggagcaaggt
     3121 caagcattag aaaatcggtt gacagaatta aaggtacccg tgcaatcctt gttttataca
     3181 gatacaaaaa aagagattac ccatgaatac caatttgatt acacgctaaa agaatcgcaa
     3241 aattgttacc agcagacatt ggattttgtg aataagtata agtaagaggt tgtgaaaaag
     3301 ccgtttcgtt ttgagtatga agaaacgaag cggcttttca caacctctct ttcaatacct
     3361 ttttgttctt gggataaaac tttccctaac aatcaatcgt gcaggataag tcaaaatgat
     3421 tggttcgtcc cgtataccgt caattcgctc caatgccatt cgaacagctg cttgcccaat
     3481 tgatttcgca ttgatttgaa cactggtcaa gccaggggag aggtttgcga ttgtctcgat
     3541 attatcaaag cttgctacaa caatctcttc tccaatttgg atattttttt gtttcaatgc
     3601 ccgatagaca ccaattgcca ttggatcgct cccgacaaga atggcagtag gaagagggga
     3661 ttcattttct aatagattag tcgcaagtac ctcaccttgt ttttctgtcc agccacctag
     3721 atgataagac atatattttt ctaagtgatg atcgatcata aaatgctgat aggcacgcat
     3781 cctcttttca tttatagaaa tctgcttccc accgaattca tccatatcga ctttatatcc
     3841 cccgatatag gctattcttt tatgaccatt ttctaagaac aattccagta cggttttagt
     3901 catccgttca agatctgcat acaccatatc gacctcttgc tggatgtcag ggttatccac
     3961 gacgactatt tgcatgtttt gttttaacaa ggcggtaatc gattctttcg taacagtccc
     4021 aacaacgatc accgcaccta actgatctag atcttcccat tgtttaggat tatcagatag
     4081 attatagatt cgattcatcc ctaaatgcag acgtcgagct tcttcttcca tccctactcg
     4141 caaatgtttg taatagatat cctgttcttc gctcgctttg ccgatcgtag agatcacacc
     4201 aatcgaaggc attctctttt tgatatattt acgagtactg atgtcataat ccaacgcagc
     4261 agctgcctct actatccgtt gccgagtagt ctgagccaca tggatcgtct gatcgttatt
     4321 caacacacgg gaaactgtag caggagagac atttgccttt cgtgcgacat ctctaattcc
     4381 aaccattttt acacactcct caaaaaaata aaaaattttt attgacaaaa ttgtttagta
     4441 aacagtatac aggaattata aaaaaaagta aacggtttcc gttttgttgg gagtgaaatg
     4501 atggatatta cgcatagaaa aacagataaa aatttcatct tatttgatga aaagagccga
     4561 gtcttccatt tacgaaatac atttctttct tatctgataa agatagaaga aagcaatgta
     4621 ttggcgcata tttattttgg caaaccagtc aagcagtaca aagacaataa aaattatccc
     4681 cgaagagatc gtggattttc aggaaatgtt cccttaaatc ctgaccggtc tctgtcgaaa
     4741 gatacactac cgcaagaata ctcttctcat ggcagcatgg actttcgaac gcccgcatcg
     4801 atcattcagc gcaagaatgg atcagactta ctggatctga gatatgacag ccattatatt
     4861 acagatggga agccagagat cgaaggcttg cctcaaactt atgtgatgga caagtcagag
     4921 gcacaaacgt tggtgatttc acttaaggat agagaaacag cgatttattt cgatttgttc
     4981 tataccatat ttactgatag agcagtgatc acacgatctg taaagataag aaatgaaacc
     5041 ggagaaacaa tcaaactaga aaaagcggct agtttccagc tagactttgc ccatactagg
     5101 cgatttgatg aagtcattgc tttgccagga gcacatgtca atgaaagaca gatttcacgg
     5161 caatctgttt tatctggaac aaaagtattt gaaagccgtc gaggaacgag cagtcaccat
     5221 atgaataact ttattgcttt agttcatcat catacgacag aaaatactgg agaagcgatc
     5281 ggacttcaat ttgtttattc cggcaaccat tctttcgaac ttgaaaaaga tcagatcaat
     5341 caattgagag tcgtcggtgg gatcaacagc catcgttttt cgtgggaatt gaatgcaggc
     5401 cagtcttttc aaacacctga aatgatcctt tcttactcaa gtcagggatt gaacaaaatg
     5461 agtcagatcc accatgagtt gctacgtgaa agaatcgcaa gaggtcgtca tcaatttgct
     5521 gaacggccga ttctggtcaa taactgggaa gccacttact ttgacttcaa tagtgaaaaa
     5581 atcaaggcga tcattgatga agcaaaggag ctaggcatcg aaatgttcgt attagatgat
     5641 ggctggtttg gaaaaagaga tgcggacaat tcttcattag gagactggtt cgagtatgaa
     5701 ggcaagttga caaatggatt acgtgaaata gcagattatg ctcatagcaa aggattgaaa
     5761 tttggtttat ggtttgaacc tgaaatgatc tcggttgatt ctgaactgta tcgaacacat
     5821 cctgattttc ttatgcagga gccaggtaga atgccttctg cttccagatc acaacatgta
     5881 ctggatttta caaggttaga tgttcggcag acgattgaga aacaaatgag aaagatttta
     5941 gatacgattc ctcttgacta catcaaatgg gatatgaacc gttcgctttc agatgtctat
     6001 tcgattaccc ttgacccaca gagacaagga gaagtagcgc atcgttatat gctcgggctt
     6061 tatgaactac ttgaacattt gacaactgat tatccggaaa tcttatggga aggctgttct
     6121 ggtggaggtg gacggtttga tgccgggttt atttattata tgccgcagtc atggacaagt
     6181 gataacacgg atgcagtgga gcgtatgaaa atccaatatg gaacaagcct tgcttatccg
     6241 atttcgtcca tcacagctca tgtatcagct gtaccaaatc atcagaccga cagatctact
     6301 tcgttgaaaa caagaggaga aacagctatg agcgctgttt ttggttatga actggatttg
     6361 acaaagctgt ctcctgaaga agaaaagcaa gtaaaagacc aaattatatc ctatcaaacg
     6421 attcgtccgg tgatccagta tggccattat tatcgtttgg cgagtccttt tgaagaaaat
     6481 attgccgcat ggatgtttgt gtctcctaaa caagatgaag ctattgtgtt tttagggagg
     6541 atactagctt cagctcaacc agcgttccat gaagtatatc tgatggggtt agatgatgag
     6601 gcactttatc aggaacagac ctcgaagcgg atattttcgg gggccgaatt gatgacagtt
     6661 ggactttact tccccgattt tcaaggtgat ttccaaacag aactgcttca tttcaaaaag
     6721 ttatgagaga gaaggaaaaa agtatgaaag caaatataaa atggttagat gatccacaag
     6781 tttttagagt gaaccaatca aatgcacata gcgatcatgt ttgttatgca acaagggaag
     6841 aagtagaaac cacatcctcc cttaaacagt ctttaaatgg atcatggcgt ttttgttatt
     6901 cgccaaatcc ttcggtacga ccaaaaacat tttataaaaa agattttgat gcctgttctt
     6961 ttgatgaaat cactgttcct agtcatattg aattagcaaa ttatgacaag atccattaca
     7021 tcaatacgat gtatccgtgg gagggacatg tatttcgccg tccccctttc acgacaagcc
     7081 ctgagaaaga ggcggaaggg atcttttcag ctgctaaaga caatccggtt ggttcgtatt
     7141 tgacgacttt tcgattagaa gaaggtctaa agggaaaaca aatctcgatt cattttgctg
     7201 gtgtagaaca agcaatgttt atttggttga atggtcattt tatcgggtat gccgaagatt
     7261 cgttcactcc gtctgaattt gatttaacac cttatttgga agaagaaaat attctagctg
     7321 tggaggttta caaaagaagt acagctgcat tcttggaaga tcaggacttt ttccgatttt
     7381 ttgggatctt cagagatgtc ttcttagttg ctcgaggaca aaaccattta gaagatcttt
     7441 ggatcaaacc tcgcttagat gacaaatgtc aaaatggcaa acttgaagta gatttgtctt
     7501 ttgagcaagt agccaaaaac agtcagctga atgtagcgat actagatcct gatgggaagg
     7561 aaatctatca acagcaatat ccgttaaaag agcaaatgac gattcgcatc gatggatttg
     7621 aaaatgtggc actgtggggg cattttcagc ccaatttata taagcttgaa ttggagacga
     7681 tagacgaaga aaaaagagta acggagtata ttgactattc cttcggtttt cgtaaaatcg
     7741 aaatcaaaga taaaatcatc tacttcaatg gaaaacggat gattatcaac ggggtaaatc
     7801 gacatgagtg gcatccgaaa aaaggacgcg ccatcactat agaagatatg gaaaaagatt
     7861 tggctatcat aaaaaatgca aagatcaacg gcgtacggac atctcattac cccaaccaga
     7921 tcacttggta ttatttatgc gaccaagcag gaatccatgt gatggcggaa acgaacctcg
     7981 aatcccatgg ctcatggcaa aaaatgggcg catttgagcc ttcttacaac gtaccaggtg
     8041 cagtacctca gtggcaggcg gctgttcttg atcgagcaaa aacgaatttc gaaacattaa
     8101 aaaaccatcc aagtattctt ttttggtctc ttggaaatga atcttatgcg gaagaaaaca
     8161 ttcgacaaat gaacgattac ttcaaaaaag tagaccccga tcgacttgtc cattatgaag
     8221 ggaattttcc aaatccagca ttcgaagatt cgatttcaga tgtaaaaagc tgcatgtatg
     8281 cgccaccaga agagatcatt gactaccttg aaaataaagc ggaaaaaccg ttcatcttat
     8341 gtgaatatat gcatgacatg gggaattcgt taggtggaat ggattcttac atgacgttgc
     8401 ttgatcgtta tgaaatgtac caaggaggct atatatggga ttttaaagat caggcgatcc
     8461 aagtgactga tccaatcaca ggcagagaag tgatgcgcta tggcggtgac tttgacgata
     8521 aaccaagtga ctatgaattc tcagggaatg ggatcgtttt tgcagatgga caagaaaaac
     8581 ccgccatgca ggaggtaaga tattattatg aaaaatacag taaataaaag tcatatggat
     8641 acggaaaaag ttgcaatcgt cttcggcgac tgtacattag gtgtcaaatc ggggaatacg
     8701 cattatattt tttcttatac aagaggcgga ctggaatcgc tcaataaaaa cggaaaagaa
     8761 tggctatacc gggaaacact tccagttttt tggcgagcaa cgaccgataa tgacagaggt
     8821 agcggatttg gatttaaatc cggtatgtgg ttaggagccg gattgtatcc aaaagtcaaa
     8881 gaaatagaag tactggtgga caataaaaag atcgatttgc cgattgctcc agtaaataat
     8941 caatattcaa gtaatgaata cgctgaaacg gttgaaatca tttttacact ggaaatcaac
     9001 acagtaccaa aaacccaagt gacaatttcc taccaaatcg ctggagaggg taaaatgctt
     9061 gtcaatatgc attattatgg acaaaaagga ttaccggaat tacccgcatt gggtttaagg
     9121 ttcattatgc caacgaaagc aaaatttttt gagtatgaag gcctatcagg ggaaacatat
     9181 ccggatcgga tggctggtgg tgtcgaaggt atttataaag tggcaggact ccccgtttcc
     9241 ccataccttg ttccccaaga atgcggcatg catatggaaa cgaaatgggt agaactaaca
     9301 agaaatcaaa cgttggataa tacggataaa gatcaagaag atttttcgtt gcgatttgaa
     9361 cagcaggata cgccatttgc tttttccgct ttaccattca aagcagaaga actggaacag
     9421 gcaacacata tagaagaact accgccagaa agacgaacgg ttcttacgat ttttgcaaaa
     9481 gtaagaggag tcgggggtat caatagctgg ggagcggatg ttgagtcacc ttaccgaatc
     9541 agcagcgaag aggaccatga aatcggcttt attattcagt agcaatcttt agattattat
     9601 ttgaaaaacg gagtttacaa gacaaagctg ctttattcta tatgtcatga acaagagagt
     9661 aaaaaagttc tcagatactt ttgttcatga ctattttctt caataaaacc aattgttatt
     9721 tttctgaaaa acaaagttaa ctatcagaaa attaaaaaaa gtttcataaa
//
LOCUS       DAYYBG010000099         9725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000099 DAYYBG010000000
VERSION     DAYYBG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.100"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            292..498
                     /locus_tag="ACHAUN_002369"
     CDS             292..498
                     /locus_tag="ACHAUN_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8461037.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(700..1701)
                     /locus_tag="ACHAUN_002370"
     CDS             complement(700..1701)
                     /locus_tag="ACHAUN_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8461038.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1706..3619)
                     /locus_tag="ACHAUN_002371"
     CDS             complement(1706..3619)
                     /locus_tag="ACHAUN_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8461039.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3787..4293)
                     /locus_tag="ACHAUN_002372"
     CDS             complement(3787..4293)
                     /locus_tag="ACHAUN_002372"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8461040.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4453..4893
                     /locus_tag="ACHAUN_002373"
     CDS             4453..4893
                     /locus_tag="ACHAUN_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8461041.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4919..6034)
                     /locus_tag="ACHAUN_002374"
     CDS             complement(4919..6034)
                     /locus_tag="ACHAUN_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461042.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6079..6435)
                     /locus_tag="ACHAUN_002375"
     CDS             complement(6079..6435)
                     /locus_tag="ACHAUN_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461043.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6733..7707)
                     /gene="sgrA"
                     /locus_tag="ACHAUN_002376"
     CDS             complement(6733..7707)
                     /gene="sgrA"
                     /locus_tag="ACHAUN_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8461044.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7903..8742)
                     /locus_tag="ACHAUN_002377"
     CDS             complement(7903..8742)
                     /locus_tag="ACHAUN_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8461045.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8927..>9725
                     /locus_tag="ACHAUN_002378"
     CDS             8927..>9725
                     /locus_tag="ACHAUN_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8461046.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLD"
BASE COUNT         2912 a         1954 c         1463 g         3396 t
ORIGIN      
        1 tatgattgtt atgtaaaata aaaaagttta ggatacatcg aatttttggg gaaattcttt
       61 tgggggtatc ctaaatcttt tctattatat tcccatattt tctattattg gtctagacgt
      121 atgatggtaa actttgtacc tatctgattg ctgaatcatt aattgcttat cattagccat
      181 ctttgtttca agttcgctag atgatcattt tattcacatc ctaacaaact tgtcacattt
      241 atagattctt aatctattta tcatacaatt ataaaaaagg aggtgctgct tatgaaagct
      301 atccatattt ctttgctttt tttcgctagt gcattgattt tatttgctat tcatctttgt
      361 tcccagcctt atatcaataa caacggaata ttagtggaac cagcattctt ttgcttacca
      421 ctcggctttt tgtcccttct tgcctccgca ggcttcggat ttgttgcttt ttttaaacga
      481 tcaaaacaac aaatctgact tataaaattt acgtaaaaga gatcgtgaca ataatcgtgt
      541 gatattccca aatttcgaat aaaaggtgcc ccttgccatc tctaactcct cagtcacaag
      601 ttacaatatt cataaaccat gaaaagcttg tttattgaaa ttgctccttg tgacttggag
      661 ctaagtgaca gattacaacc tcttttgcat cataagactt tactccttga aaaacattac
      721 taacttttga tatgtttctt gcgcttgttt attgctgaaa tcaaactgat attcatggtt
      781 caaaacagca tttttctccg cataatctac ttttgtgact ttcacccccg ctttctcaaa
      841 tacagaagtt acttcttcca tttgtttcgt aaacgtattt gtatttccat ccgttaagaa
      901 tgtgttttgc gtccaacgat ccgcatgtct gaccaaggct aaacttttga ccttctctga
      961 ttgttcaaag tccttattat caaagtaagc ccatccacat ttttcataaa gaaagctaga
     1021 gattttatca cttacttgag taagtgcaga aggatccaac agaccagaca aagaaataaa
     1081 cttcgtaatc tgcttgttct ccaaagtcgg atcaagatga tcagcttttt taatttcttc
     1141 attttgaagt gctaacaaat attctccact aatttgtgca cctgccgaat ttccaccaat
     1201 cgaaatattc gaccaatctg cttgatcttt tatctctttt ttagctgctt gaatggcttc
     1261 gttcaattgg ataactggtg taggatgttt tgcttccggt gatcgttcat aatctatatt
     1321 tacgatgatt tgctgcgtgt ctgaacatag atattccaaa taatcacgac aatcattttt
     1381 atcccctcct acatatgcgc ctccatgcat ccagaaaaga atcggtaatt ttttcttttc
     1441 cactttcggt gtgtagatat ccatattatt atttttgtac tttgaaggat aggatatttc
     1501 tttttttata tcgatttgtt cgattttttg ctggaaatca tctggtcggg catatacttg
     1561 ttctttttca cttgtgtcaa attgttttct aacaagcaag ctaatcggct ttggtgtgat
     1621 ttgattaatc aaaaatacag tgactaaaat aactacgatg ataactggta ctgcaatcac
     1681 tattcgcttg aaccatttca ttcatttacc ctctctttct tcgccaaatc catgtgataa
     1741 tcgtgctaac agataaaaat ccacctgaat aaatgagaag cgaatgtttc ttttctccag
     1801 ttttcggcaa cattcttttt tttgcatctg tctttttttg tactttcact gtcatctgaa
     1861 tcggttgcgt atcatcactt gattgatcct ctcgaggttc attctttccc ttattgtctg
     1921 cattctctgt cggatcttgc ttttttggtg gagctactgg gatttttttg taatagaaaa
     1981 taatagttgg tatcgtctca ttaaaaatcc cttcagtcgg tccttcaaca tttgctaatt
     2041 cgtatccttc gaatgtcttt tgctctgtca tataattttc tccataagct ccgtaaagct
     2101 ggatttcgtc ctggtctttg agtagacttt tattgttttc atccacatac tttacgttta
     2161 tctgatcatg gaacactact ttcaaaaagt ccgttacatg tttcgtgtag gcagcatggt
     2221 caacttcctg tgcctttcca tgccctgctc cttctactaa taaaagatct ttataccctc
     2281 ctgataactc atataatttt tgcgcgttag acacaggtac tacatcatct gctgttccat
     2341 ggataatcaa tttaggcaat gaatctgaca ttccaatcgt aggtagagca gcttttgtat
     2401 ccaaatgcat ctcctgatca aaatagtatt gtttcaaaag gcccataaat aaatcttcat
     2461 gctcttgatc cccaatgatg tctaactgat tttttatggc atccggtagt gcgggggcta
     2521 ctgtctggct gtacgtaaaa cgtagttgtt catcaattga tgcaaatccg caattttcaa
     2581 taattccttt gacattgaca ggaatatctt tcgctaatac agacatcatc gtagctgctc
     2641 ccatcgaacc accataaagt aaaatctttt gagatggccg ctctcgaact tcttgattta
     2701 tccagtaaag gacgtcatct gactcatatt gtccataggt cacataatcg cctccgctat
     2761 taccagtcgc tctggaatca acaaataata aattataccc ggccttataa aaatcattgt
     2821 attcttctgt aacgatcccg ttattccaat tccctcgaaa tccgccatgg atcatgactg
     2881 ttttatctga accttgatcg acataatatg catatacatc catcattttg ccatcttgtt
     2941 tgtttagtac agtttgatga agttgttttg tatttggata gaagcctgtg ttttctgtag
     3001 gcgttgtccc atcatctgta cctccaagca tcgacatcaa cacactgttt tttgcaaaag
     3061 cgatatcgta caatagataa gtagcaatca gatgtttgat aatctcttta tctacaccat
     3121 aatcattaac agcaagctca gtgactaata actctattaa ttttgacttg atgtcttctg
     3181 ttggtccatt aagtatttct gtcatctgtt taccgaagta attcaaatat tctttaccat
     3241 tgaaattttc acctttaccg atatcatcat tttctgtccc attctctttg tcacttactt
     3301 tagcagaaga ggattcgtcg tttgacattt ttgtagctgt tgcttcttct gtttgttggt
     3361 tatctgtggt agcggtcgtt tcttgatctg tgctagtaac agatgtctca accgttgttt
     3421 cagtatttgt ttctgtttgt gaagtactcg atttcgtttc atttccagca tcagtagttt
     3481 ctgtggtttc tacctcccga gattccgatt cggaagaaat gctctctcca tatactgtcc
     3541 attgcgggac gcttgtactt acagcaaata agatgaccaa cgctcccttt aatacttgtt
     3601 ttgttctcaa gctattcact cctttttatg tttctaggag aattatatgt aaacgcaaac
     3661 attttttata caaaaaataa atggtttatc ccataaaaat agaacaggat tattttctca
     3721 ttttacgtaa taaagcagaa tggataaacc agtatccatt ctgctttttt taaacgaaga
     3781 aacaacttat tgctccaaaa atgtcagaaa tcgtttatcc caaattttct cgtaaaaagc
     3841 gattgtttcc tttgctttca taaaaggatt atcataagtt gtcgttgctc ctctacacat
     3901 cataagctgg tatcctaatc catgagcaaa cttcacagtc gtatctatac aatactctgc
     3961 ttgtgcccca cataattcga tcgaatgaca gtccaattca gttaataaaa cactcaaatt
     4021 agtcttataa aatgaattcg catgggtttt tctcacaaaa taactgtgct caggaataga
     4081 taattccgac aatagctccc atggttttgt ttgtggcact aattgagcat cttcgtgttg
     4141 aacgaaaata actggtttcc caatcgtttc gtatgttttg attcttttat tcactaatgc
     4201 tgtcactgtt tctaattcgt gtatcggcga agaatcaaaa catacccctt tttgtaaatc
     4261 gatcactact attgcgtcgg ctgtgtttga catacttgtg ccttctttct tttttcttga
     4321 tttaatagta tttcttatct agctagttcc attatacgtg acaacatttt cttattttac
     4381 acctctaaat ggcacttttc gactatttct tcatgttatt atttcataaa tacttagcta
     4441 ttggaggaat gtatgatgat taaattgtat acaggttctg gctgctcgtc ttcaagacgc
     4501 gcaaaacgtt ggcttatcgt ccatgatata ccgtttatcg aacagaactt aattcgtcaa
     4561 ccactaacca aaaatgaatt tatccactta ctaaaattga caaacaacgg cgtgaccgaa
     4621 atgctctcta ctcattctat tagttataaa aatcttgttc acagaataga tgaactcagt
     4681 ttgagtgaac tatatattga gattcgtaca tccccttctt tattgaaaaa accgattatc
     4741 tacgaccaat ctgcaaaact tttgactgga tttagcgaag ataaaatcag gatgttcctt
     4801 tctaaaacta aaagaaagca agagctcaac gaactcttgc tttctggtca tcttgtcgat
     4861 tttaaagaaa ataatctttt tgcaatgtct taattttggc ataaacaatc tgcttttctt
     4921 aattaacgaa atccaatttt tccaatatgg atctattttt ctcattcctg atttttatga
     4981 tttcagcttt cagacgcgcc caatcatttt ttgtaggttg tccattccag taaaatatct
     5041 gtgtattcgt ttgatcatat gcctttttgt cgataggata atcgctaatg acaatatcag
     5101 gtaactgatc agcggatgca ctatatttta ttgggcgttc agtcatagat aatatccttt
     5161 tctttgccca taattcctgt gttttaccat actttgattg aatatagaat atgacaggtt
     5221 gttcgtgttt tataaaaata tccctgacta acatacagta ttgaaaaatc aaccgtctgt
     5281 tcttttcata ataagtatta agctctggaa ttctgcgagc taccccacta aaaaactgtt
     5341 gcatgattgg atacatcact ggaaatatat gctgaaaatc tctgtcttct gctgattttc
     5401 caaaagcatc caccttcttt tttgtcccaa aaacccttga atacaaatgg atcattgcca
     5461 aattgacata tagataattt ttttctgaag ttgtaaaaca aatcttgaag tttttttcaa
     5521 aattttctac aatagacgat tcaatccagc cccaactttc ttgtacttta tctggagtat
     5581 ccaacaaatc ttcttccaaa taggtgttca ttacaccaaa cataaaataa aggtaagctt
     5641 gctctgttgg agtgatgtga ttcatcgacc agaagtttgc ttcaaaaaaa ggctctagta
     5701 tgtaatgcgt aaactcttct tttgttatca atggatgcac aaatttgttc atctcttctg
     5761 ggacatacgt aatcgagtgg ccttgtttca ttcgttgcaa cgaaattctt agtaggctga
     5821 tccatttctt tctatctata ctacgcgtat atggcgcatg tttcgcaaat ttttctaata
     5881 gttgacgttt attttttata cttgataata tatcagaatg attttcgtcg aatgactgcc
     5941 aatacatcaa gtgaaaaaag tatcggatac ggtattcttc tccttctatc ggattacctt
     6001 ttttcaagct gcaagataaa tgatagtttt tcaaaatagt attaagttct cttaattttc
     6061 tttgaatcgt acgcaaactt atataatttt ttgactaaga ttttctaagg attgataagt
     6121 acctttgtaa agtgaatcca gtaaaatata accaatcgtt ccctctaaaa gtatagaata
     6181 aactttactc agtaaaaact gagaagaaaa ctcgactttt atcatcttgt tttctgaaag
     6241 aaaagataat ttatattctg gatacctttc gcaaaacgat tctgcttgtt gaatcaccga
     6301 attcatctta gccagggtgt agtttagatc taaagcgata tccttcacca catataattt
     6361 ctcgtctctt tcataacaat agttcaaaaa ttgatatact tgcctttcat tgatatcaat
     6421 aaattcttca aacaaagtac atactccttt ttatctatta tttagactta ttccgacagc
     6481 tacatgaata acgtacaaat agattcgtta ctgatttttc agagatacta ccatgcgcag
     6541 tgtgcattgt caattattct tgctcttatt ttccatttgt gttcctgtta tttcctgttt
     6601 ctcaaatatg ttagtaaaca aaaatagatc tggcaaagct acaacttcgc caggcaatac
     6661 agcactctcg aatgaaatat tcactcaaaa atctatcgtt ttattcaatt ttagatctgt
     6721 tgcttttatt ctttatttca ctcgatggaa gagagaacga aaatagcttg ctcctacaat
     6781 caaaatgagt aacacaccca tccatgtaat tagcttgttc aaggttctac taccagtttc
     6841 tggtaaatcg ctttttctat tatctgtttc tttcgctttt tcttgatgat caattccacg
     6901 agaaacaaaa tcaggctgtt ttagcacact ttttaggttg ctccctgcat ttctcttttg
     6961 ttccttagtt ggtatgatag gtaccaaatc aagttcatta tttacttttc ctgtgctgct
     7021 ttcgctgctc gtgcttcctg tgctactctc actactcatg cttcctgtgc tactttcact
     7081 actcgtactc tccgtactgc tttcgctgct cgtgcttcct gtgctacttt cactactcgt
     7141 actctccgta ctgctttcgc tgctcgtgtt ttctgtgcta ctttcactgc tcgtactctc
     7201 tgtactgctt tcaccgctcg tgttttctgt actgctttca ctacttgtgc tatccgtact
     7261 gcttggcggt ccaacaactg tccaactgct tggtttcaaa ttctccgtga tcgtcatctg
     7321 tccatcataa aaaacaatca aatcaacttt atctttactc ttgatctcat caatcgatag
     7381 ttctgcagaa atcattattg tatactcaat accagcggtc cattgtacgt cttttaaata
     7441 aacttcccca ataacttttt ctgaacttcc attaccatcc caaccatatg ttagaacagc
     7501 atgtacatca ctagcattca cgcttttgtt tggtttgact ggaatagtaa tgacttgttg
     7561 tgtcatgtca taaacaatcc gatcagtttt cacagtaaat gaaagattct catttgcccg
     7621 ttcatttgca gaagtactga ttccaatcat tgctgcaatt atactcagcc caagaataac
     7681 aaataatacg gtcgctgttt tcttcatttc tatcactctc catataatta ccttctattt
     7741 gataaaggaa ttatataaaa tgaaaatgat gagaacacga cagtcagttg tgaaaatcta
     7801 acaaaagatg tcgtgtattc gcttgaattt gtttactttt tcgtttaatc aatgacagac
     7861 ttaaaaaagc aagaccgata tcagccttgc tttcttcatc ttttattgat aaacagcagt
     7921 gtatttcttc atccgctttg ttttatatgc atagagtaac aaaccaacaa tcaagactaa
     7981 caagaataat gtagttgata tagatgcttc agccccaaag tttcccccag taattattgt
     8041 attttgcgat tggatcagag tggagaaaaa accttgttct tgcgttccgc tgactggtat
     8101 acccaaaata gctccttgtg tccaattcca agtaatatga gcaccaatag ccgtaaataa
     8161 acttcctgta ctgtagataa gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac
     8221 tgccagaaat gtcatgttag gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc
     8281 aatcattgct attaacggac caaatctaag catctgatgc tgaaaaaagc cacggaataa
     8341 aagctcttct gatgccccca ctgcaataaa taaaataaaa ctcgttatca gcggcacaat
     8401 gtttgcgttt cctgaatcta accgatacgc ccccgtcaaa tataatggac tagtaataag
     8461 cagaaacact ccaattccaa caacgagacc tcccgctaaa tacccccagt cttttcctcg
     8521 aacagaaaca ccataatctt ctattctttt tccttcgacc ttactagcaa gaaataaagc
     8581 tagtaaaaaa acagctaaat gtccgaccgt accggcaatt gctccaatcc attcactatt
     8641 tccaataaac atatccgcaa gtgcattatt gacaagaatt cctaaaacaa aaccaactaa
     8701 gactattaaa acagatccaa tcaaccgaat aatcctgatc atctttcatc cctcctttat
     8761 attaagttta acaacccttt tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc
     8821 atagagagaa tagattttta cgaactaaga atatctacag caaccttacg aaaatgaaag
     8881 actgtctccc taattcttct gttagactgt ttttaataag gaaaggatgg gaaatttttt
     8941 gaaaaaacaa ctactaatta tgtgcctttc agcatgtgca attttactat tctcaggttg
     9001 tagcaagtcc aatgaaacga ttgcaacgtt taaaggtgga acacttacag ttcaggattt
     9061 ttatgataac ccaaaagtta aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt
     9121 caaagcattt gatgatgtat acggaaaaga gatttcttca aaggaagtaa caaaagaata
     9181 taatgaacaa atcaaaaaac tcggtccaaa ctacaaggaa caattaaaag cagttggcca
     9241 aacagaagag acatataaga aactctttaa acagatgctg gctttccaat acggtttgaa
     9301 agccaatgta aaattaacag ataaagatct agacactgct tggaaagaat tctatccaga
     9361 agttagtaca cagatcattc tattttcgac taaagaagaa gcagataaag caaaaaaaga
     9421 agcaaatgaa ggagaaaatt tctccaagct tgttcaagct tatggaaaaa ataaaacatt
     9481 aaaggaaact gatggaaaaa tgaattttga ttcaaccaat ccagagattc ctactgaagt
     9541 aaaaaaagca gcatttaaac taaaaaacgg ggaagtttct gatatcattc ctgtaacaga
     9601 tccaacgact tatcagcaaa gctattatct agtaaaaatg gtaaaaaaac aggataaagg
     9661 ttctaataaa gacaaataca aatctgagct agaaaaaatt gctaccgaag caaaaactct
     9721 tgata
//
LOCUS       DAYYBG010000100         9629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000100 DAYYBG010000000
VERSION     DAYYBG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.101"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..364)
                     /locus_tag="ACHAUN_002379"
     CDS             complement(170..364)
                     /locus_tag="ACHAUN_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461047.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(377..559)
                     /locus_tag="ACHAUN_002380"
     CDS             complement(377..559)
                     /locus_tag="ACHAUN_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461048.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(556..696)
                     /locus_tag="ACHAUN_002381"
     CDS             complement(556..696)
                     /locus_tag="ACHAUN_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461049.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(1186..1551)
                     /locus_tag="ACHAUN_002382"
     CDS             complement(1186..1551)
                     /locus_tag="ACHAUN_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461050.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(1591..1893)
                     /locus_tag="ACHAUN_002383"
     CDS             complement(1591..1893)
                     /locus_tag="ACHAUN_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8461051.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(1893..2192)
                     /locus_tag="ACHAUN_002384"
     CDS             complement(1893..2192)
                     /locus_tag="ACHAUN_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461052.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(2185..2349)
                     /locus_tag="ACHAUN_002385"
     CDS             complement(2185..2349)
                     /locus_tag="ACHAUN_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461053.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(2355..2624)
                     /locus_tag="ACHAUN_002386"
     CDS             complement(2355..2624)
                     /locus_tag="ACHAUN_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461054.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(2636..3385)
                     /locus_tag="ACHAUN_002387"
     CDS             complement(2636..3385)
                     /locus_tag="ACHAUN_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461055.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(3382..4074)
                     /locus_tag="ACHAUN_002388"
     CDS             complement(3382..4074)
                     /locus_tag="ACHAUN_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8461056.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(4080..4751)
                     /locus_tag="ACHAUN_002389"
     CDS             complement(4080..4751)
                     /locus_tag="ACHAUN_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8461057.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(4744..5085)
                     /locus_tag="ACHAUN_002390"
     CDS             complement(4744..5085)
                     /locus_tag="ACHAUN_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461058.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(5263..5961)
                     /locus_tag="ACHAUN_002391"
     CDS             complement(5263..5961)
                     /locus_tag="ACHAUN_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8461059.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            6048..6518
                     /locus_tag="ACHAUN_002392"
     CDS             6048..6518
                     /locus_tag="ACHAUN_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461060.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(6561..6740)
                     /locus_tag="ACHAUN_002393"
     CDS             complement(6561..6740)
                     /locus_tag="ACHAUN_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461061.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(6727..7065)
                     /locus_tag="ACHAUN_002394"
     CDS             complement(6727..7065)
                     /locus_tag="ACHAUN_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8461062.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(7081..7284)
                     /locus_tag="ACHAUN_002395"
     CDS             complement(7081..7284)
                     /locus_tag="ACHAUN_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461063.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(7299..8075)
                     /locus_tag="ACHAUN_002396"
     CDS             complement(7299..8075)
                     /locus_tag="ACHAUN_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8461064.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            8374..8694
                     /locus_tag="ACHAUN_002397"
     CDS             8374..8694
                     /locus_tag="ACHAUN_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8461065.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            8712..9143
                     /locus_tag="ACHAUN_002398"
     CDS             8712..9143
                     /locus_tag="ACHAUN_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8461066.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            9273..>9629
                     /locus_tag="ACHAUN_002399"
     CDS             9273..>9629
                     /locus_tag="ACHAUN_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8461067.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQ"
BASE COUNT         2759 a         1894 c         1491 g         3485 t
ORIGIN      
        1 attctccaga attattttct aattttaccg aagacgacat tcctactctt aaacaattaa
       61 aatattattc tgattattgg acgcaaactc gtgagaatga attaaattta gaaaaactca
      121 ttattaaaaa gaaatagcaa ctccagccca cacggctggt tttttagttt caacttccct
      181 ccttacgggt caaaatcatc ggttccggca taatctgatc atcctttagc aacaaatcag
      241 gatatttttt ccttagcttc ttattgttcg tgtaagtttt caatagccat tggttggctt
      301 cgcttttgct ttctccagaa aaataaattt tccctgtttg gatgcctgca agttgaatca
      361 tcattttcac ctcaaatcaa attttttctg ctagatggag caaaagcaat ccgacatcaa
      421 gaatcttttg acccattatt tcagctactt tgctcggctt aactccttga gaaaagagca
      481 agcgtgcttg ctctatctct tcgtgagtcc aaataaattt ggcttcctca aggataatta
      541 ctggatcagt tgctctcatt gttcctctgg aaaaatgatg tcattcttat gcttggacca
      601 ggtatctgca tatggaacaa agtattgtct agctaattcg atttgatcaa ttaaagcagt
      661 ttctgataat tcatggtctg cagcaatttc attcatacta tcgccttcat ctaaacagat
      721 aagaccgttg acgttatcga tttttacatt tgtgactgct tgactttctc cacgctcata
      781 atattctaca gttgatactg atacatcagc ttcggagttt aattgcagaa tctgttactg
      841 ccgggataat ttaatattct tttcataatt cacctttttt cctactgaca tcgaatattt
      901 ttcatgttat gattagccgt gaaaggagag ctatattatg tgtttacaca tgatgtcctt
      961 agctcattcg gttctttgga agcttttttg gaaataactc tcaggttctt gacgcaaatc
     1021 tcatttattc tttgaaacaa aaaacttcca ttttccgaga gctttggatc acttcaaaag
     1081 agatttgcat ttatatttct gtgaccttga gtcgcgtaaa gaaccttaca gcaatggcgg
     1141 caattccact ttcaggaaac cgcctttttt cattgatatt ggtggttagc ggactaccca
     1201 ttttctttcg gcagtgccaa accacaaatg atctgtgttg atgtttaggt agttgtatac
     1261 cagataagct aaatccaaag tgcctgcatc ttcaagacga caatattcca aaaattctcc
     1321 catagctgga cataaaaatc ctggagctat catatcccaa cagttaatag tgcaaagaaa
     1381 ttcgtttagc ttctgaacgt tttctttggt ttcacgaatt acaacatact ccaattcttc
     1441 tccgctggca gagcattcca aaacttcaaa ttgattaaga aatggtagaa catatgcttc
     1501 aaacatcaac attccatctt tctgtttttg cacttgtttt gcatcaatca tgtctatcct
     1561 cctattcagt tatttcttcc attaatggaa ttatttcagg tcactggact taacgaaaac
     1621 accattgacc atctttccag tccgaccttt gatttcgtca taagcaaaat ccaagcactc
     1681 gtataaatcc atatcatttt gcattgccaa gatgatcaag gttactacta catcgccaat
     1741 tccgtctctt aaaccatgtt catcttttct agctagagaa gcggcaactt ccccgatttc
     1801 ttcgaccgtt tttaacattt gcttgctgaa atccgcttga tccaatccct tatcttttgc
     1861 ccactgctct actttcgcga ttagttcgtc cattatttct cctccacata cctaaactgt
     1921 cttccttttg aatcaatcca taagctccta gctctatccc aaataatatt tttgctcaga
     1981 ccagtaattt cagataactg tttagcggta cccgttacta gaattcgatc accatgccag
     2041 attgcaatct ttctcggcgt tctccgtttg ggcttttcag tccacattga tttaccgagc
     2101 ttttggactt ctgcaactat ttttttatct tcctgccaat cttctgaata agtcaattcg
     2161 ataattcgct gcattgccgc tttcttatcc atcccgacat tctcctttca gtaatttgag
     2221 tacttgatca agtgcgctct cacgtccgcc atgaaatgtg ttgagccact tgtcttcgta
     2281 cgacacactt tgtcttaaag cttcttgatg cattaattcg atctgtgctg taaatgtctt
     2341 taggtccatc ttgtttacac ctgctcaagt tcattaagat gtttttgcaa gcctttaaca
     2401 caatcaatga atagtaattt ggtatatgct aaatttctta attgcgttcc atcgatatag
     2461 agtgcgaaat agtatctgag tttactccaa ctcgaacgat cattcttaat tccttcaatt
     2521 ccagcttctt cgagttgatc atatacgtct ctcaggacgt ctaattcctc gcccttttta
     2581 taattcgcta tttcattaat tagttctaga taatcgatct tcatttttcc acctcttaga
     2641 atggtgcttt tgattgtcta ttagctcgtt ctagcgcttt tttcttgaga taggcttctt
     2701 ggtcgattgc ccattcagga agcttctctc gtcttcctgt gcgcttgtat ccactgcttg
     2761 cgttcttagg ttcacttttt tctttccttg cccaacttcg aatagttgcc aaatagtttt
     2821 tataagtctt accagatgat tcacaatact ctgacagtcg ttcgattcgc tcttggtaat
     2881 cattagggaa ttctgttttg agtttctcca tctgctcatc tgacaaaaga acatttttat
     2941 actctccgta tttatgacgg acgggcttag ccttcgattt tttcgaaggc ggtaactctc
     3001 ttatatattc ttttgtatta ttaaatgtat tattaataga tgtattatta tctttgactt
     3061 tttcgtcaat aggggtattg cgtttttcgt caatagggta ttgattaatt cgtaggtacc
     3121 tattgattat ttgattggta ccctctttgt aaatgatttc ccgattcaag tatccaaact
     3181 taatcaaatc acttacccat cgcgatatgg tctctttatt cacaccatat aaatctgcaa
     3241 agtactcatt gcctgcccaa caaaagcctc tttcattaca caaggccgtt atctctccgt
     3301 ataacaactt agtatttggt ttaagtcttt tgtcgtacct tacgttggct ggtataatcg
     3361 cataataact tcgatgttct gtcatttttc ccctccaata tttaactttt tgattgtttt
     3421 ctggtttaat ttgatccctt tgatttgata tttatttttg aaattaatca cacctatttt
     3481 gtgcttctct gtgtgatgga ttctgcagag tgctgcaaat gtgtactctg catgatcaac
     3541 ttctttgcgc tttcgtcttc ctagcgcttt gtcaaagtga tcgatgtcag ctcctgtttt
     3601 gccacagatg cagcagactc tttttgtgat gcatttgtag aagtaatatt cttgattcgc
     3661 aggtaaaatc tcatagcctt ctttgaaagg aatatgatgt tcaaagatga aatctaagat
     3721 gatatttgct aagacattgg catcactcac agttgtattc gattcgtctt tcaggcttat
     3781 tttgcgccct gtgacacctt caaaacgaaa gtagaagaat tccttccaga agtccgttgg
     3841 catgcctgta tcgataaaaa tatcgcctat cagcgcatag atgaagtttc gttgctgtac
     3901 ggtgaaacgt ctaggatcaa taaaacgaat ttcaatgact cgatcaccat cgtagccgtc
     3961 atacatcgtc tttagtcgat caatgttcac ttcctcattg atggttgcgc ttatgtcttt
     4021 tcctttgaac tttttcagaa ccgctgaata tgaatcgatt aatggtttaa acactcatat
     4081 cacttcttat ctaattcttt tctcttagct gctattgctc gctccatcaa ggcacattgc
     4141 tcatagctta actgttcaat agtttcaacg ttatcagcta agagccctaa tttatctgtc
     4201 tgctcattaa catattcgat taaggttttg gtcatatctt tacccatctg ctcattgaaa
     4261 gcttctagaa tcgtctctag catgtttaat ttctttgtat cgattctagg tggtgttgga
     4321 atatcttccc cttgaaatac atataatccc agtccgtgta gagccaatgc tttcacaaag
     4381 catcgcttca atgagttatt gatttgcatt gcatttggtt taacaactgg ttggtttcga
     4441 taatctaaaa caggaaataa ttcggtttcc gtgtgtcctt taaccgttac tgagacagat
     4501 acataagtcc cagtttcatc cataagaaaa ggtttatatt cctcaacaag aaagtcttga
     4561 tgagttccag aaacaaccct gtagtgtttg tactcattaa tagttaccgt tgcctgtgga
     4621 tcattctttt tcataatctc ccacgcgtga gcccaagata aataatcaaa atttcctttt
     4681 ttcttgagaa ttttatttaa cttacgacta aaaagttttt caaagttcgt tgtccctttg
     4741 atttcactca tcaaattctg cctccatttc agcaatgtat ttcttacctg gtccgtaata
     4801 agagatatca atcaagttat ctctgtcgta ctcttctagc gcatcaatca agccatcttc
     4861 gatgacgtaa atatattcag gtttattcga atgcttcgat agatggataa gataaacatg
     4921 atcccaaata gttacatggt tgcctaaatc atcttgatcc caagctagtt cttcattcgt
     4981 caaaagattt cgtctgattt ttcgaccact tgtttcctca attttcggct tgccccaatc
     5041 aggatcaatc aaatattgat ctagagtgga aagttctttt tccatatgct aaaatctcct
     5101 tatgatgtgt tttctttgtg actctttgct tgccggcgga gtcacttttt tatttgttgc
     5161 catgcttttt gcttgtcaat atgttgttgg cttaggatga ttggtttata gtatttccac
     5221 cagcaattag caattgccgt ccctattctt agcgcttcag ctctattcat tgtcatcacc
     5281 aaaaagtctc tgttgtctgt tcagttgatc aatttccata cggatcgcag tttctggcaa
     5341 ccacatttca ataaatgaaa cagcatcatc gaatctctta cgaggtaact cgccatatct
     5401 tgggattgaa aaggtacgtt taaattcaga ccaaaatttt gagaatactt ttttgctgat
     5461 ttcttcataa gctcggcttt cttttccccc tagaactccc ataactttca tatttccttt
     5521 ttgcttaatt tcaaactctt gttgtccgct aattcgcata gtatctttaa gcatggaaac
     5581 atctttttta acatctttca tttcttctaa ttgatagatc atcatatctt caattgtttg
     5641 aggaacagta ttcttgcgaa taacatcttc catttcgttg aatgcttcaa tgtatttttg
     5701 tttgaagtag atagctttct ttcctgtaaa ccccatagcc agcaagaaaa atccatctct
     5761 actaatgaag aaaactcgtc gatttctgcc atatgaatct ggttcattac cttctacaaa
     5821 catctgtcca aaattggaca catctttttt tagtgcatca atatctctta aaacatgttg
     5881 atgttttttc tcgaagcttt ctgccacttg taagctcgta gtcacagctt ctttattttt
     5941 caaaattact aattcttgca ttatttcttc tctccttttt ggtataattt agttaaaaac
     6001 tggtggtgtt tattttgaat ttttttcact taacaatttt tcaatggatg tcaataataa
     6061 gttttatatt gagtacgttt ttaacaggga taaaaataaa aggaatgcgc cctcaactag
     6121 aagttgaact taatgcttct tacttcgctg cagatatgat ttatacaaaa gtaatcattt
     6181 ctaactattc aacagaacca gctatgttag ttaatttaga actttcatcc gctaacttaa
     6241 accgcagatg gtcagctact ccatttaaga aattaattgc taaaggagga tcagtcgatg
     6301 ataatagaat atactctgaa gctgtaccgc taaatattcc tcctaaaagt gctatatctt
     6361 gttaccttgc atttgaagta gggaaaatta attttaataa actccttaat caccaaacaa
     6421 aaatgatatt tactctaaat agaacccaaa tccacaaaat tgttgatatc aaaaatacaa
     6481 acttcccaat agagaaatta gtgaaagaat taaactgagt aatagtaata tacattctag
     6541 tttctctttt ctattcattt ttagtcagcc ccctcggttg gctttttcgc tctgtactca
     6601 gcttcagctt catcaagccc cataaaaatc caaaccatgt aaacaatcgt tcctatcaac
     6661 gcttgcctgc ttccccaaag tcctaaagcg tagatgatta gcggtgcgct gaacactagc
     6721 gctctattaa attttcccat ccgcttgcct ccttaagatt tccgaaaaat ttgtttctaa
     6781 aaattcaagt gttttacttc ttaaaaatag atatgtgtct cttccttcaa ctggataata
     6841 gacaaatcca tttttgtttt tctcgatatc gataatattt ctatatcttg ggttctttaa
     6901 aactctagaa gtaaaccaat catattttct gttaatccgt tctagcactt ctggcaacgt
     6961 catccatcta ccagtatcat cagctttttt taactcctca taatccactt gggagataat
     7021 tacatagcct tctggaattg ggatttttgc ttctagatat tgcattagct gttcctccct
     7081 ttacaattcg tacatagtga taatcgaatc tataattctg tttgcttctg cagaagtctt
     7141 tttaccgttt aaaattaaag ataagtagct tttgctaatt ccaaatcttt cagcaagcat
     7201 ggtgtaagtt aagaactttg aactttcgac atattctttg attttttctc tatctcgttg
     7261 agtgatttct gcaatgtcag tcatactaaa actcctttct aaccaatttc ctctaaatcc
     7321 atttgagggt aatatccttc tttttttagt aattcgtaga taaatagacg ccctttctgt
     7381 gtccatttgg tattcattac aattttagtg ccaccatcgg ctttcgggat ctcagttgta
     7441 tgagattttg tgtatccttg gttcatgtgt tttttgcaca ataaccattg gttaccgact
     7501 tttttctgaa tacctagttt atgaagtaat ttattcatct gttgtggaga catcccataa
     7561 tctgctgcaa tctgactaat tgttactgaa tctgtagaag ataatatgct atctaaatag
     7621 gagattttag gttcgtactc tgcaatcttt tgttctgcga ttaatcttcc agttctttct
     7681 tcttttagtt gagttgctaa ttgaatgatt gtatctggat taagcaaagc ttcttctact
     7741 ttttctggag ttagataacc tccatgtttt ctaattgctg gcaacacttc acttgttacc
     7801 caacgtttaa attttttggc agagggaagt tttgatttta agattaaact gtacaaacca
     7861 gattcgttga tgattgtcat ctctcttgat tgacctgagg tcgtgattcg cgaccccatc
     7921 ttatcttcca aatctacatg ccgcgacaaa gcatctttag tgtttgaata acccaaaaca
     7981 ctcgcaacgt ctttgcctac aaaatatggt tcatcattta ctaaaattgt ccgaacttcg
     8041 ttttgttcga aattaaaaat ttgcggtgtg ttcatatttc tcattccttt cttttatatt
     8101 cgtaaacaaa tttaacaact tttttctaaa ttcaattgac agtatctgag taatactcta
     8161 tactaagagc ataattaaat aagacataaa aacattgatt ttaaagcttt cttggcggtc
     8221 tgcatttaca tatcaatagt gttttttgtt gtctttttag ttgttaaatc tgtttacaag
     8281 atatagtata gctcattact cagaattagt caaattattt ctgcgtattt ttctaaactt
     8341 tttttgtaaa catttagaaa ggttgtttta tcgatgaata cctatgaaat aataaaagac
     8401 ttggcgaaga ggaaaaagat atctatacgg cagttagaaa ttaattttgg atattctaac
     8461 ggttatctag caaagtggaa aaataatact cctaattcag atgaattaag tcggattgct
     8521 gactatttcg atgtctctgt agactacctt ctaggtagag aagaaagaga aacccctaaa
     8581 catgtggatt tatcagaaga cgatactgtt ttttcttttg atggaaaaga aatatctaag
     8641 gagacaatgc gtaaagcgat tgcaattgct aaagctttag aggaaaatga atagttggag
     8701 tgatggtttg tatgtatttg aaattgaaag aaatgttgag cgaatacaat ttgaaagtag
     8761 tttatatgga gatgaaagag cctggtttct actatccaaa acccagaatc atttttttga
     8821 atgaaaatct atacggtgaa actgcagaag cctttcattt atctcatgaa cttgcacatt
     8881 tcagcgcctc tcattttgaa ttttcagttt tgtacgatac ttctacaact tttcactcaa
     8941 aatttgaaac agaagcagat aaaatagcta ttctaatttt attaaatatc tatattgaga
     9001 acgaactgac tgatgaatct cagtttaatc tcgaaaaatt tatggagtat tattctattc
     9061 agaataagct cagatacact tgttatgctg tctgccaatg ctattttaag aaaaaatatt
     9121 cttacgcaag gcaatatgta tgaatacatc aagaattatt aaaaaaaccg atttcagcaa
     9181 aacattaaaa aagcccgtgc tgcaacacgg gctctttcct catatatgag cttctacaaa
     9241 aaaaatcata tcacagaaat gaggaagaaa aaatggaaaa agagatttta ataaaatctc
     9301 caggaaaaac cttgattaaa gtaactgatg attctatttc aattattaga aaaggattta
     9361 taaatttagt taatcaaggt attaaaggag aaaaaactat cccctttaaa aatatatcag
     9421 ccgtacaatt aaaaaaacca ggtatgagta atggttatat acaatttacc ttgctaggcg
     9481 gaaacgaaag tcgcggaggt atattagcgg ccacgaaaga tgaaaacacc gttatgttta
     9541 caaaaaaata ttggaatgaa atggaaaatt taaaaaaata tatagagaag caacaatcta
     9601 tattagataa taattccaaa gaaacacaa
//
LOCUS       DAYYBG010000101         9619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000101 DAYYBG010000000
VERSION     DAYYBG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.102"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACHAUN_002400"
     CDS             34..657
                     /locus_tag="ACHAUN_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461068.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACHAUN_002401"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACHAUN_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8461069.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACHAUN_002402"
     CDS             2153..3034
                     /locus_tag="ACHAUN_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8461070.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACHAUN_002403"
     CDS             3037..3807
                     /locus_tag="ACHAUN_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8461071.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACHAUN_002404"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACHAUN_002404"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8461072.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACHAUN_002405"
     CDS             5118..6281
                     /locus_tag="ACHAUN_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8461073.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACHAUN_002406"
     CDS             6398..6808
                     /locus_tag="ACHAUN_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8461074.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACHAUN_002407"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACHAUN_002407"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8461075.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..>9619
                     /locus_tag="ACHAUN_002408"
     CDS             9381..>9619
                     /locus_tag="ACHAUN_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461076.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIII"
BASE COUNT         3342 a         1632 c         2074 g         2571 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattattta
//
LOCUS       DAYYBG010000102         9583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000102 DAYYBG010000000
VERSION     DAYYBG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.103"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..631)
                     /locus_tag="ACHAUN_002409"
     CDS             complement(<1..631)
                     /locus_tag="ACHAUN_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8461077.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSL"
     gene            complement(633..1397)
                     /locus_tag="ACHAUN_002410"
     CDS             complement(633..1397)
                     /locus_tag="ACHAUN_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8461078.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1405..2082)
                     /locus_tag="ACHAUN_002411"
     CDS             complement(1405..2082)
                     /locus_tag="ACHAUN_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8461079.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2119..3504)
                     /locus_tag="ACHAUN_002412"
     CDS             complement(2119..3504)
                     /locus_tag="ACHAUN_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8461080.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3714..3798)
                     /gene="ffs"
                     /locus_tag="ACHAUN_002413"
     ncRNA           complement(3714..3798)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHAUN_002413"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3821..4336)
                     /gene="tadA"
                     /locus_tag="ACHAUN_002414"
     CDS             complement(3821..4336)
                     /gene="tadA"
                     /locus_tag="ACHAUN_002414"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8461081.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4443..5300
                     /locus_tag="ACHAUN_002415"
     CDS             4443..5300
                     /locus_tag="ACHAUN_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461082.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5381..5716)
                     /locus_tag="ACHAUN_002416"
     CDS             complement(5381..5716)
                     /locus_tag="ACHAUN_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461083.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5783..7153)
                     /locus_tag="ACHAUN_002417"
     CDS             complement(5783..7153)
                     /locus_tag="ACHAUN_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8461084.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7231..7767)
                     /locus_tag="ACHAUN_002418"
     CDS             complement(7231..7767)
                     /locus_tag="ACHAUN_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8461085.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8076..9557
                     /locus_tag="ACHAUN_002419"
     CDS             8076..9557
                     /locus_tag="ACHAUN_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461086.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1500 g         3206 t
ORIGIN      
        1 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
       61 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
      121 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
      181 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
      241 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
      301 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
      361 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
      421 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
      481 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
      541 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
      601 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
      661 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
      721 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
      781 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
      841 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
      901 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
      961 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     1021 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     1081 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     1141 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     1201 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     1261 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     1321 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     1381 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     1441 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     1501 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     1561 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     1621 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     1681 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     1741 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     1801 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     1861 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     1921 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     1981 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     2041 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     2101 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     2161 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     2221 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     2281 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     2341 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     2401 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     2461 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     2521 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     2581 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     2641 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     2701 ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     2761 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     2821 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     2881 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     2941 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     3001 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     3061 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     3121 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     3181 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     3241 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     3301 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     3361 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     3421 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     3481 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     3541 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     3601 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     3661 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     3721 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     3781 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     3841 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     3901 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     3961 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     4021 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     4081 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     4141 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     4201 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     4261 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     4321 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     4381 tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca
     4441 agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag
     4501 catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag
     4561 attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc
     4621 aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt caagaaaaaa
     4681 gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc
     4741 aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag
     4801 atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta
     4861 tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc
     4921 attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag
     4981 agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt
     5041 actatacaaa agatactgta aaagatagta agttgatcaa aagttacata gaactgatca
     5101 aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct
     5161 ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc
     5221 aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat
     5281 tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac
     5341 cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa
     5401 aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag
     5461 agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac
     5521 tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga
     5581 cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg
     5641 atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt
     5701 ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa
     5761 aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta
     5821 ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc
     5881 atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt
     5941 gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc
     6001 taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta
     6061 aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa
     6121 gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct
     6181 cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt
     6241 gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata
     6301 tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg
     6361 attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga
     6421 tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt
     6481 ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag
     6541 attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac
     6601 aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg
     6661 gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa
     6721 tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag
     6781 cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg
     6841 gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc
     6901 cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg
     6961 gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta
     7021 gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg
     7081 atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg
     7141 cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa
     7201 ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc
     7261 caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc
     7321 ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga
     7381 cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag
     7441 cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa
     7501 aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta
     7561 gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt
     7621 gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt
     7681 aattgacttc attccatatt cttccacgta taccatactt ttgaacagca aatattcttt
     7741 actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc tacccataaa
     7801 taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata tttagttata
     7861 tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa ataccctagg
     7921 aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata taaaaaagaa
     7981 ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag tttttgtcta
     8041 actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca agaaaaaaag
     8101 agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa gaattatcca
     8161 atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata aatgtatcac
     8221 tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga gagatccaaa
     8281 ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa ttatgctacc
     8341 taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca caaactgctg
     8401 taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc gaaaaactga
     8461 caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct gtaaaaaatt
     8521 gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt tttctttcag
     8581 atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca ctaaaaatga
     8641 acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc tacttgctgt
     8701 taactttatt catattacga aacaaacaac aggcatattt tccaaaaact tcttctgaaa
     8761 tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac tttacttctt
     8821 tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt aattattttt
     8881 tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt ggttttttat
     8941 tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact tacttaactc
     9001 aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat tttcttgtgt
     9061 tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct acactttgct
     9121 ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa ttaagagaaa
     9181 aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa taccttagtc
     9241 aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc catttgcaga
     9301 taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag ttatttcgca
     9361 aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt acagatcagc
     9421 atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac aatacagtgt
     9481 tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt tatgagaaca
     9541 aaacaaagag gatgtgacaa aattcttgtc acatcctctt att
//
LOCUS       DAYYBG010000103         9439 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000103 DAYYBG010000000
VERSION     DAYYBG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.104"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACHAUN_002420"
     CDS             168..362
                     /locus_tag="ACHAUN_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461087.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACHAUN_002421"
     CDS             631..930
                     /locus_tag="ACHAUN_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461088.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACHAUN_002422"
     CDS             complement(1027..1461)
                     /locus_tag="ACHAUN_002422"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8461089.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACHAUN_002423"
     CDS             complement(1693..2403)
                     /locus_tag="ACHAUN_002423"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8461090.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACHAUN_002424"
     CDS             complement(2393..3253)
                     /locus_tag="ACHAUN_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8461091.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACHAUN_002425"
     CDS             complement(3258..3896)
                     /locus_tag="ACHAUN_002425"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8461092.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACHAUN_002426"
     CDS             complement(3911..4210)
                     /locus_tag="ACHAUN_002426"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8461093.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACHAUN_002427"
     CDS             complement(4253..5737)
                     /locus_tag="ACHAUN_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8461094.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACHAUN_002428"
     CDS             complement(5757..6218)
                     /locus_tag="ACHAUN_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8461095.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACHAUN_002429"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACHAUN_002429"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8461096.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACHAUN_002430"
     CDS             7521..8291
                     /locus_tag="ACHAUN_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8461097.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACHAUN_002431"
     CDS             complement(8564..9391)
                     /locus_tag="ACHAUN_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461098.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2930 a         1934 c         1481 g         3094 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagacccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacac
//
LOCUS       DAYYBG010000104         9110 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000104 DAYYBG010000000
VERSION     DAYYBG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.106"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..1718
                     /locus_tag="ACHAUN_002432"
     CDS             87..1718
                     /locus_tag="ACHAUN_002432"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8461099.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1721..2800
                     /gene="uxuA"
                     /locus_tag="ACHAUN_002433"
     CDS             1721..2800
                     /gene="uxuA"
                     /locus_tag="ACHAUN_002433"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8461100.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2874..4301
                     /gene="uxaC"
                     /locus_tag="ACHAUN_002434"
     CDS             2874..4301
                     /gene="uxaC"
                     /locus_tag="ACHAUN_002434"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8461101.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4385..5032
                     /locus_tag="ACHAUN_002435"
     CDS             4385..5032
                     /locus_tag="ACHAUN_002435"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8461102.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5152..6528
                     /locus_tag="ACHAUN_002436"
     CDS             5152..6528
                     /locus_tag="ACHAUN_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8461103.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            6538..7332
                     /locus_tag="ACHAUN_002437"
     CDS             6538..7332
                     /locus_tag="ACHAUN_002437"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8461104.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            7345..>9110
                     /locus_tag="ACHAUN_002438"
     CDS             7345..>9110
                     /locus_tag="ACHAUN_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8461105.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         3167 a         1182 c         1858 g         2903 t
ORIGIN      
        1 ttcccttttt atattgacaa cggttacaat gggttttaaa atataaaatg tatcaaaact
       61 aacatatcag tttgaggagg ttaattatgg tttcgattaa aaattggcaa aattctatcg
      121 aagaattcaa tgaaatgcag attctactgc cgtgttttga tgtcaaagaa gttaagcaag
      181 aaggccagca acatccagta tggcttcatt ttggtggagg aaatctttat cgaggatttc
      241 atgcagaaat tgctcaatgc ttagcagatc aaaatgaatt agaaacagga gtagttgtgt
      301 gtgaaacatt tgatgaagaa gtagtggaaa aagcttatca tgactataac aatgacattt
      361 tggaaattgt tatgagtgga gatggtgaac tcaaaaaaag acttttggca gtaacggctg
      421 atagtttata ttgcaatccg aagaatatag cggactatca aagagtggtt aaatattttg
      481 aagatcctaa attacagttt acaacgttta ctattactga aaaaggttac agtttaaaag
      541 attctaaagg gcaattcttt tcggtagtca aagctgatat caaatctgga cctagcaatc
      601 ctcaacatac gatgagtatt gtaacatcct tgttatatag cagatttaat gctggagagt
      661 tgccgattgc gatggtttct actgataatt tctcacagaa tggtcaacgt tttcaagata
      721 gtatttttac gattgcagaa gcttggcaaa aagaaggata tgtttctgaa aaatttattg
      781 cttatttaaa ggataaagaa aaagtgactt ttccttggag tatgattgat cgaattacgc
      841 caaatccttc agagaatgta aaggaagatc tattgatgat ggggctcgag gatttagata
      901 tcattcacac aaaaaaacat accaatattg ctgctttcac taatactgag gtagttcatt
      961 atttagttat tgaagatagt tttccaaatg gccggcctaa attggaaaag gctggtgtga
     1021 ttatgacaga tagagagacg gttgataaag cagacatcat gaaggttacg acaagcttga
     1081 atccacttca tactgctttg gcagtaacag gttgtctgct aggatatcaa tcaatttcag
     1141 cagagatgca ggatgatgat ttggttggtt taatcaaagg aataggttat ttagaaggtt
     1201 taccagtagt tgaaaatcca ggcattattg atccaaaaca atttattgat gaagtaatta
     1261 ataaacgttt gcctaatccc tatattccag atacaccaca acgaattgca acagatactt
     1321 ctcaaaaagt agcgattcgc tatggtgaaa caattaaaaa gtatgtcgaa aaagacggaa
     1381 atgcagaaaa tctaacattt attcctatcg ctattgctgc ttggttacgt tatttactgg
     1441 cagtcgatga tcaaggggaa aattttgtgc caagtcctga tccgttattg gtagagttgc
     1501 aggataaatt atcagtattg aaattaggtg aacaagatcc ttcaaagatt cattgtgcgg
     1561 tacaggaaat tttagaaaat aaagaaatct ttggtttaga tttatatcaa gttggggtag
     1621 gaaagaaaac tgaaaaaatc ttttgcgaaa tgctagtagg tcctcaagca gttcgtaaaa
     1681 caatcaataa atatataaca gaagttggag gaaagtaact atggaaatga cttttagatg
     1741 gtatggggca aatgaagaaa aaatctcact acaagaaatt cgtcagatcc cagcaatgaa
     1801 aggaattgtt ggaacattaa tggatatacc agccggagag gtttggccga gagaacgaat
     1861 taaatcttta aaagatgaag tcgaagccaa tggacttacc ttgaaagtta ttgagagtgt
     1921 taatgttcat gatgatatca aaattggttt accaacacga gatcaataca ttgagaatta
     1981 taaacagacg attcgcaatt tggcagaatt tgggattgag gttatctgtt ataattttat
     2041 gccagtattt gattgggtaa aatcggattt ggattatcgt ttagatgatg ggtcttctac
     2101 gttagcctat atttcaaaaa atatccctac tgatccggag gaaattatta gagaagtaga
     2161 aaattctact aataattttg aattgccggg ttgggaacct catcggttat cacaaattaa
     2221 gcaattatta gaagcctata aagaagttga tgaagagaaa ttacgagaga actttgcata
     2281 ttttctcaaa gcaattatcc cgacatgtga agaagttggg attaaaatgg cagttcaccc
     2341 agatgatcca ccatattcca ttttcggatt acctcgtatt gttaaaaatc gtgacgattt
     2401 agattggttg tgtaatgtag tggattctcc ttctaatggt attacattat gtactggaag
     2461 tattgcagaa cgaccagata atgatgtcta tgaaattcta tctgaatttt gtcaacggga
     2521 tcggattcct tttgcacatg ttcgtaatat caaatattta agtgacaatg gactcgattt
     2581 ttacgaagcg ccgcataaat cagaatatgg ttctctagat atgtataaga tattaaaaac
     2641 aatgtacgat aatggattta atggctatat tcgtccagat catggtcgga tgatttgggg
     2701 agaaaccggt agacctggtt atggattata tgaccgagct ctaggtgcag tttatttaaa
     2761 tggtatttgg gaagcgcttg aaaagggggc gagtaaataa ttatataagg gagtttattt
     2821 agaggaaaaa ttttctcttc taaaaactaa cggaaggaaa ggagtgttta aatatgtttt
     2881 tagataagaa ctttttatta gagaatgaaa tagctaaaag actttttcat aaatttgcaa
     2941 aacaccaacc aattatcgac tatcattgtc atttagaccc agcatatttg ctagaaaata
     3001 agcaatacaa gaatttgact caggtatggt taaatgataa aggagctgga gatcattata
     3061 aatggcgatt gatgcgagct aatggtatag aagaagagtt gatcagtggt ccaggtgatg
     3121 attatgaaaa atttttagct tttgtaaaaa cagtggagaa ggcgccaggt aatcctattt
     3181 ttgaatggtc tcatttggaa ttacaacgtt actttgatat tgatttgaca atttctgaga
     3241 agaatgcacc agaaatttgg cgacgtgcta acgaaaaaat tcaaacggat tcctatcgcc
     3301 cgaaagaatt gttggagatg atggatgtga aagccatctg tacaacggat gatcctacag
     3361 atgacttgtg gtatcatact gagctttcta aacaggataa ttctttcaag gtattaccta
     3421 ctttccgtcc agataaggtc tggagtatca aatcatcagg cttttcgaac tatattttaa
     3481 aacttagtca aacagcggct ataccaatca aagactttaa tgggttgaag caagcattga
     3541 gccaacgagt ggatcatttt catcaaattg gctgtcgatt agcagatcac ggtttgaata
     3601 cttattttta tgaaatagca gatgaaaaaa tactcgacaa tctgttaata aaagctttag
     3661 caggaaaaac ggatttccat ttatcagaaa ttgcacaatt tgttacaggt attcaattac
     3721 atttgatgtc tgagtataca agatatggtt ggacgatgca aatgcattta aatgctttaa
     3781 gaaataattc aagtatatta aaggaacaaa ttggagtaga tgtcggtggt gattcgatgg
     3841 gggatcaagc tagtttgaca caaaatgttg tagcattgtt cgataccgct gaaaagagtg
     3901 ggtgcttacc aaaaacaatt ttatattcat taaatccaac tgattggctt ccactagcaa
     3961 cagcaatgca aagcttccaa gggggtatga ttcaaaaaat ccaattggga tgtgcttggt
     4021 ggttcaatga tacagctgaa ggaatgcgtc aacagttgac tacgatggca caacaaagtt
     4081 tgttgtcgaa ttttacaggt atgttgactg attctcgaag ttttctaagc tatcctcgcc
     4141 atgagtattt tcgtcgagta ttatgtcaat tactaagcga gtgggttaaa gcaggccgaa
     4201 ttccagatga tgaaatgtat cttagtcaga tagtggagaa tatttgttat cgtaatgcgt
     4261 ataattattt taattttttt gaaatcggaa gtagaaaata atacttgaaa taaaagctta
     4321 aatttattct ttgtaagtta gtacagctag acaaagtatg tatccataaa ggaaggagat
     4381 agatatgaaa aaagtagaaa ttttaagtaa gttagaagcg gctggagtca ttgcggttgt
     4441 acgtgggaaa aataaggatg aggccattaa agcaataaat tcaattattg cgggtggaat
     4501 taaagggatt gaactaactt ttacagttcc taatgctgaa gaaataatca aagaaattgc
     4561 taaaaaatat gctgatcaaa aagatgtaat aatcggagcc ggtacggttt tagatgcgtt
     4621 tactgctcgt atcgcaatta tggctggggc agaatatatc gttagtccat cttttgatcg
     4681 acaaacagct gagatttgta atttatatca aattccttat ttaccaggtt gtatgacaat
     4741 tactgagata aaggaggcac ttactgctgg cgtagatatc attaaactat tcccaggttc
     4801 tgcttttggt ccatccattg taaaagccta tcaagctcca ttaccacacg taaatataat
     4861 gcctactggc ggagtaagta tagagaactt agaagattgg tttgaagtag gagttgttgc
     4921 agttggtgta ggtgggaatt tattggcttc tgttgaaaaa ggtgattttg aagaagtaag
     4981 tcgagtagct agtaaatata tagataagtt ttatgagata aagaattatt aggagaaaaa
     5041 acaaataata ctctgaaatg taaaaaataa atactgactc tttacgcttc agagtatagt
     5101 acgtactttc atcagaatga aaacgcttaa caacagaagg gagagaattg tatggaaaga
     5161 aaatttggta taagagacaa aataggatat atgtttggag attttggaaa cgattttact
     5221 ttcatttttg ctagttcttt tttaatgatc ttttatacaa aagtattggg gataagcgga
     5281 gctgccgttg gaacattatt tttagtagca agatttatcg atgcagttac tgatgtaact
     5341 atggggcgta ttgttgatag atcgaaagca actaagcagg gtaagtttaa accgtggatt
     5401 ttaagaatga gtggtccagt cgctttagca agttttttaa tgtatcaaac ttctatgatt
     5461 tcagcttcta tgagcgttaa ggtagtatac atgtatgtta cgtatatatt atgggggagt
     5521 attttttaca catctataaa tattccttat ggatcaatgg catcagctat tacttctatt
     5581 acagaagaaa gaacaatgct atcgacattc cgaaatgttg gagctacttt agcaagtttg
     5641 gtcattggtg tattaacacc attatttatt tatacaagag atgattcagg cgctcaaata
     5701 gttcgtggtg gatcaacttt tacaattgtt gcgggaatat tttcgatatg tgcacttatt
     5761 tgttatttaa tatgttataa actaactact gaacgagtaa gaattgacca atctcaatac
     5821 aaagtttcat tgttaaaatc tttaaaaggg ttagttaaaa atagggcatt actttctata
     5881 attggtgcgg ctattttctt actccttgct caattattaa ttatgagtat gaataattat
     5941 gtatttcctg attactataa tgatgcacag ggaatagttt taatgaattt tatcaatcct
     6001 attttggttt taatagttgt ttcgccttta actttgtttt taagtaaaaa atatggaaaa
     6061 aaagaactgg catcagtagg aatgttcttt tcagcaatag tatatttgat cttatttttg
     6121 ataaaagcta gtaatatgta tgtattctta attcttagtt cggttggcta tatgggtttg
     6181 ggtgtgttta atactgttat ttgggcaaat attactgatg ttattgatga tcaagaagta
     6241 aagtctgatc aaagagaaga tgggaccgta tatgcggtat attcttttgc tagaaaaata
     6301 ggtcaagctc tagctggagg cgtcggaggt tggactcttt ctattatagg ctatgactct
     6361 ttagcaaatg tacaaacggg agatgtttta gaaaagttat ataacgcttc aacattaatt
     6421 ccatcaattt gttttctgat tgtaggatta attttatttt tcctttatcc attatctaaa
     6481 gataaagtct tagaaaattg cgagctttta aaacaaaaac ataaatagga gagaaatatg
     6541 aataattgga tattttgtat tgattcagat ggctgtgtta tggatacgat gacttataaa
     6601 cacaatctct ttttcggacc aattgctgca gagatttttg atgttcatca tagagaaaaa
     6661 ttcctacagg attggaataa tattaattta tattcagaga ctcgtggaat aaacagattt
     6721 ttaggtttag ttaaaacatt agaaaaaaat gatgtaacat cgattaatcg attaaagcac
     6781 tgggtatctc taacaaaaga attgtctaat ccatctttga agagggaaat agaatattct
     6841 cagtcagagg atttattaaa agctttaaaa tggtcggaag aagtcaatca acagattagt
     6901 caagcaaaag gattagataa acccttttta agtgctagag atactataaa agctctgaaa
     6961 aaatatggga agataattat tgtaagctca gctaataagg aagctgttca agaagaatgg
     7021 gaaagacatg aattattatc attagttgat gaactgtgtt gtcaagataa aggaaaaaaa
     7081 gaagatatta ttcgaagtgt aattgaaaat ggttgtgatt tggataagat attaatgatt
     7141 ggtgattctc ctggtgatct agaagctgca aataaaaata aagttttctt ttatcctatt
     7201 ttggtcaata aagaaaaaga atcatgggaa aacttaagaa ctgatgtttt atttaaatgt
     7261 ttatcgggag attatgtaga tattgaacaa aaatatatag atactttttg gaaaaatctt
     7321 actaataagt agaggagaaa gaaaatgact ataaaagtag atttacgaaa aaaaccatat
     7381 aatttagatg aagatgctat ttcttggatt gaagagacga ttgattctat gactttggaa
     7441 gaaaaaatag gtcaattgtt tgttaatatg ggatctagtc gtgataaaaa ttacttgagt
     7501 aatatggtca atcagtacca tattggagct gtacgatata atccaggaac tgctgatgag
     7561 gtatatgatc aaaactatat cttacaaaca gaaagtaaaa ttcctctatt aattgctgct
     7621 aatacggaag caggtggtaa cggagcttgt acagatggta ctgaagttgg tgtagaagta
     7681 aaaattggtg caacaaatga tgcgaaatat gcttatgaat tagggagagt ttctgggatt
     7741 gaagcatcgg ctattggatg taactggagt tttgctccta ttgtagatat taatagaaat
     7801 tggagaaatc ctatcatttc tagtcgatct tttggacaaa accctgataa agttttagaa
     7861 cttagtttag cttatatgaa aggtattcag gagagtggaa ttttgcctgc agctaaacat
     7921 ttccctggtg acggtattga cgaacgagat cagcatctat cttttagtat taatagttta
     7981 ggtacggaag aatgggataa ttcttttgga aaagtatact caggcttaat agaagctgga
     8041 ttaccttcaa ttatggcagg tcatatccat atgccagctt attcaaaaca ttttaatcct
     8101 gatctgaaag atgaagattt acttcctgca actttatcaa aagaattaat cactgactta
     8161 cttagaggaa aactcggatt taatggatta gtagttactg atgctagtca tatgttagga
     8221 atgactgggg caatgaaacg atcagaatta ttgcccactt ctattgcagc tggatgtgat
     8281 ctatttctat tttttaatga tccagatgaa gactttaatt atatgatgga aggctatcgt
     8341 aaaggaatta tcactgatga tcggttatta gatgccttaa aaagaatttt agggttaaaa
     8401 gctaaattgg gattacacaa aaaagcaaaa gaacaaattt tattacctaa gcaagaagct
     8461 ctggaaaaaa ttggtttaat tgaaaataaa tcaattttta aagaggtggc tgaccaagca
     8521 attacattag ttaaagataa acaagatatt tggcctattg taccagaaaa atataaaagg
     8581 attttactag tagatgtaca tggggtaaaa ggtgggtttg gtgaagtagt tgctggaaat
     8641 agacctcaag ccattgattt attgaaacag attttgtcag caaaaggatt tgaagttgaa
     8701 aaatacattt cacctgaaga agaattgaaa ggattatcgc aagaagaaaa aattaaaaat
     8761 atagctggaa tatatgctgc taagagacca attaaaaatt taactaaaaa atatgacttg
     8821 atcattaata tagctaatgt taatccaggc acagttcaac gaattgtctg gccagcaagt
     8881 aaaggaactc cggatatacc gttttacatt catgaaattc caacaatttt tgtatctgta
     8941 cagtgtccgt ttcatttggc tgatgtacct caagtaaaga cttatattaa tacttatgat
     9001 ggaaaacaag aaactttaga aatattggtg gacaaactaa tgggtctttc tgaatttaag
     9061 ggagtaagtc caatagatgc ttattgtggt ttaaaggata catttattta
//
LOCUS       DAYYBG010000105         8833 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000105 DAYYBG010000000
VERSION     DAYYBG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.107"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..183
                     /locus_tag="ACHAUN_002439"
     CDS             <1..183
                     /locus_tag="ACHAUN_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8461106.1"
                     /translation="QLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTSLLIISLV
                     IYFLKIKNRIIDDYNS"
     gene            196..1203
                     /locus_tag="ACHAUN_002440"
     CDS             196..1203
                     /locus_tag="ACHAUN_002440"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8461107.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1215..2219
                     /gene="neuB"
                     /locus_tag="ACHAUN_002441"
     CDS             1215..2219
                     /gene="neuB"
                     /locus_tag="ACHAUN_002441"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8461108.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2216..3364
                     /gene="neuC"
                     /locus_tag="ACHAUN_002442"
     CDS             2216..3364
                     /gene="neuC"
                     /locus_tag="ACHAUN_002442"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8461109.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3337..4014
                     /locus_tag="ACHAUN_002443"
     CDS             3337..4014
                     /locus_tag="ACHAUN_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8461110.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3965..5146
                     /locus_tag="ACHAUN_002444"
     CDS             3965..5146
                     /locus_tag="ACHAUN_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8461111.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5159..6136
                     /locus_tag="ACHAUN_002445"
     CDS             5159..6136
                     /locus_tag="ACHAUN_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8461112.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6136..7044
                     /locus_tag="ACHAUN_002446"
     CDS             6136..7044
                     /locus_tag="ACHAUN_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFM8461113.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            7045..7797
                     /locus_tag="ACHAUN_002447"
     CDS             7045..7797
                     /locus_tag="ACHAUN_002447"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8461114.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            7802..8749
                     /locus_tag="ACHAUN_002448"
     CDS             7802..8749
                     /locus_tag="ACHAUN_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8461115.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         3248 a         1114 c         1680 g         2791 t
ORIGIN      
        1 caattatttg acacaaaaaa aatatgtatc ataataattt ttgtatcaat tttttctttg
       61 atacttcata ttttaatcaa tcaaatgttg attcgttacg ttttgacatc attattgata
      121 attagtttag ttatatattt tttaaagatt aagaatcgaa ttatagatga ctataattca
      181 taggaggaga tataagtgtt taaaggaaaa acaatattag taactggcgg aactggttcc
      241 attggaagtg aaattgttcg tcagctttta aaatatgaac caaaagctat cagaatttat
      301 agtagaggtg aagaaaaaca attttatatg caacaagagc tctcaaatca cactaatttg
      361 agatttttaa ttggtgatat cagagactct caaaggattg attatgcatg tcgtgatgtg
      421 gatattattt ttcatgctgc cgcaatgaaa cacgttccag caagtgaata taatcctatg
      481 gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa tagatgcagc gatcaaaaat
      541 aatgtaaaac attttgtagg tatcagtaca gataaagtag tgagtcctac taacaccatg
      601 ggtgcgacaa agttattgag tgaaaaatta ataatagctg ccaatcagta taaaggaaaa
      661 acagaaacaa tattttcttg tgtaaggttt ggaaatgtca tggggtcaag tggatctgtt
      721 attcctcttt ttagtaaaca aataaaaaac aataaagata ttactttgac agatgttgat
      781 atgacacgat ttatgatgtc aataccggat gctgcaagat tagttctgtc tgctgctatg
      841 aattcaaaat ttggagaaac atatgtctta aaaatgccgg caatcaatat tttaacttta
      901 gctcaatcat taataaaaat atataatgat acacatgatg aaaaatatag aggtgaaata
      961 aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag agctaatgac tacagacgaa
     1021 attgaacgct catatgaaaa tgataaaatg tatatcattc caccacacta tcatgctact
     1081 gacgaactgt acaaagatta tgaaaaaatt aaaatcactg catactcttc agatgcaaca
     1141 gaattaatga gtggacagga atttattaga atcgcaaaag agaataatgt cgtgacttgg
     1201 taaggagttt tatgatgaaa tcagaagtat atgtaattgc agaaattggt gtaaaccata
     1261 atggaagtct agatttagct ttagaaagta ttgataaagc taaagaatgt ggagctaacg
     1321 cggttaaatt tcaaacattt aaaacgaata agctagtttc caaaaaggct ccaatggcaa
     1381 aatatcagaa ggataatctt aaagaggaca gttctcaatt tgaaatgcta aaaaaattag
     1441 aattatctca atctgatttt attagaataa aagattattg tatacaaaat aaaattgatt
     1501 ttttgtctac tccttttgat gaagaaagtg cagactttct aaaacaaatt ggtgtacaag
     1561 gatttaaaat tggctcagga gatttaacaa atttaccttt tcttagaaaa atagatatgt
     1621 atgggttgcc agttttatta tctacaggta tggctgatat tcatgaggtc agtgaggctg
     1681 tagattgttt catagatagt ccagttacta ttttacattg tacttcaaat tatccggcta
     1741 gttctgaaga tattaattta ttggcgatta acacattaaa agaaatgttc gggatacccg
     1801 ttgggtactc cgatcattca ttgggatatg atgttggtat ttgtgctgtt gcgatgggtg
     1861 ctaaggtaat tgagaaacat tttaccttgg ataaggaact acctggtcca gatcataaag
     1921 cttctttaga tcctcaggaa tttgcagatt ttgtaaagca tattagaaac actgaaatat
     1981 ttttaggtga tggtgttaag cgagcaatgc cttctgaact tagtacaaaa gaagtatcaa
     2041 gaaaaagcct agtggtcact gagaatttaa atgtgggaga tattcttaca gagaaatctc
     2101 ttacaataaa acgtcctgga aatggaatag ctcctaagtt tttatatcaa tatattggca
     2161 aaaaagttaa aagaccaata agtgcagata gtgttctgct tgaggaagat gttgaatgaa
     2221 aaaaatactt atagtaagtg gaacacgagc tgattatgga atatataaac ctgttgctga
     2281 gaatttacta caagataatt gggatgtatc atttgttgtt tctggaatgc atttatccca
     2341 taaatatgga tttactaaag aattgatctt agctgacgga tttagaatta gtggagaagt
     2401 tcaaagtttg tttttagaga acaataaggg aaacatggct cggtctgtgg cattagagat
     2461 aaatggtttc acaaatatat ttgaacaaac tagccctgat tttgtattat tactagggga
     2521 tagaggagaa atgttggcag ctgcgatagc ttgtttgtat cttggaatca aaacagcaca
     2581 tattcacgga ggcgaagtgt ctggtactgt agatgaatca ataagacatg ccataacgaa
     2641 aatagcatct gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa taaaaatggg
     2701 ggaagattct tggagagttt ttcccgtcgg agctcctaga atagatacta ttttaaataa
     2761 tagattacct agttttagag atataaaaga aaaatatcat ctaaaatttg aaaagaaaaa
     2821 atacgttttg caagtatttc atccagttgt aaccgaacta gaaaatatag aaaaacaaat
     2881 tttagctttg atcaattcaa tggaacaaca atctcaacca attatttgca ttttacctaa
     2941 tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa aaacggatat
     3001 tatttatatt acgaatttag agccggagga atacctgact atattaaaag agtgcaaatt
     3061 tatgatcgga aattcttctt ctgggataat tgaagctgca tcattcaaaa taccagtaat
     3121 aaatttagga accagacaaa atggacgaga acaatctaaa aatattataa atgcaaatct
     3181 aaatgaaaat gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt ttataaaaag
     3241 cttgaaaaat gttaagaaca tctacggtga cggcaacagt tctagaagaa taacaaaaac
     3301 attgaatcaa atattgaatg atggggaaga tattaagtgg attcaaaaac aaatagctta
     3361 ttaaaatata ctagtgatcc atacattatt attggggctg gcggacacgc aaaagtcatt
     3421 attgaaacct taagattaga aaataagtta atatatggat tttttgatga taagcaaaat
     3481 aaaatgcaag atctgaaata tcttgggaaa ataaacgaat tgaatagctg ggcggatagc
     3541 aaaaaaaaaa tctataaatt tattgtagct attggtgact gtgtagtaag aaaaaagatt
     3601 ataaaaaaat tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc aagtgctata
     3661 gtggcaaaag aaatttcaat tggttatgga actgttattt ttgcgaatgc tgtaattaac
     3721 ccagatgctg taattggaga gcatgctata ataaacacag gtagcattgt tgagcatgat
     3781 tgtagaatta ataactatgt tcatttatct ccaggtgtat gcttagcggg tggagtacat
     3841 gtaggtgtag gaactcaaat aggaattggt tctcaatgta tccagatgaa gaaaattgga
     3901 tcttggtgta tgataggcgc agggagcact attgttaagg atattccctc acattcctta
     3961 gcttatggca atccagctaa aattaagaaa gaaggaatta attttgaatt ctagaatttt
     4021 attatcaagt cctcatatgg gaggaacaga agaaaaatat gttgctaagg cttttaaaga
     4081 taattggata gcgccattag gtgaaaatgt ggataaattt gaagaaagta ttacaaaata
     4141 tactggttct aagggcggag cttctgcgtt aagttcaggt acagctgcca tacatcttgc
     4201 cttaagactg cttaatgttg gaaaaaacga tattgtcttt gctccttcat ttacttttat
     4261 agctacagta aatcctattt tatatcaagg ggctgtacca gtgttcattg actcagaacc
     4321 taatacatgg aatatgagcc ctgttgcact agagagcgca ttagaagaag ctgaaagaaa
     4381 caaccaatta cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg ctgattatga
     4441 acgaatttta gctattgcaa accaatttaa tgttccagtg atagaagatg cagcggaatc
     4501 gttaggttct acttataagg gaatttcgtc aggcttattt gggacaattg ggatctattc
     4561 atttaacggt aataagatca tcacaacctc aggtggagga gctctagtgt ctagtgataa
     4621 aaaatatgtt gagcattcaa gatttttagc aacacaagca agagatcaag caccgtatta
     4681 tcaacatagc gaaataggtt ataattaccg tctaagcaat atttctgccg gtatcggacg
     4741 tggacaaatg gaagtattga atgatagagt agctaaacga agagaaattt tcaatcgata
     4801 taaggaagct ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg atggcatggt
     4861 gccaaatcgt tggttgtcta ctttaacgat caatgattat aattctattg atcaagtagt
     4921 taacgtaata aataaactaa atgaaaaaaa cattgaaaca agagctttgt ggaaaccact
     4981 acatttgcaa ccagtatttc aaggggtaaa aatgtattac catgataata atcttagtgt
     5041 gtcagaaaag ttatttaata gcggcttatg cttaccttct ggaagtaata tgacagatag
     5101 tgaacaagaa tttgttattg atgagtttaa aaaaatattc aagtaggtgt gatattttat
     5161 gaacatagga attattggtt taggttccat ggggaaaaga agacttagac tcattcaaga
     5221 acactttaat gatattttac tatgtggtgt tgatactaat gaggcgagac gaaaggaagt
     5281 tgaaaaggaa tttggaatac caacttataa tagtctaaca gaggcaaaaa atttgtttga
     5341 aataaattca gtttttgttt gtacatcccc tataacccat gaatttatta taaaagaggc
     5401 tttaaattta gattgtaata tctttacgga aatcaattta ttaaatgaat attatgatga
     5461 aataatcgaa gtagctaaaa agaaagatag attactttat ctatcttcta catttttgaa
     5521 gagaaaagaa attcaatata ttaagtctaa agtgacgcat aataaaaaaa tttcttatca
     5581 ataccatgta ggacagtatt tacccgactg gcatccttgg gaagattatc gcaatttttt
     5641 tgtttcaaac aaaaaaacta atgggtgtag agaaatattc gctattgaat taccttggat
     5701 tatagataca tttgggaaag ttaaatcgtt tactgtaatg acccagaaat tatccaatct
     5761 tgatattgat tattcggaca gtttcactct tattattgag catgaaactg gtatagttgg
     5821 aactttaagt gtagatgttg tttcaagagt agctaaaaga aatttaacta taattggtga
     5881 aaagatttta atagaatggg aaggtaaccc ggattctcta ttttcatgga atgaagaaac
     5941 ttctagtatg acagatattc aattgtacga tgaggttatg catgataata attattcaca
     6001 tactattatt gaagatgcat atttagagga gattaaagaa tttttcacgc ttttggaaaa
     6061 gaaaatggaa ccccaatata cttttgaaaa agataagtat attattaact taattaacga
     6121 cattgaggta aattaatgat gaaagtaatt ttttttgggt tagggtcaat tggaaagaga
     6181 catctaaaaa atctaataag ctatggaaaa aagaattcta ttaattttga tataacagct
     6241 tatagtagca gtggaaaaaa agagtttgaa gaaataacgt atattcaaaa tagagacgat
     6301 ttggatacac attatgatat agcttttatt acaaatccga cttttaagca tctggaaaca
     6361 ctaaatgtaa ttgttgaaaa agttgattac atatttttag aaaaacctgc tttttctgag
     6421 agtattgatc ttactcaata taattatgat tacaaaaata tttatgttgc tgctccactg
     6481 aggtataaaa atatcatgga agttgttcgt aatagtattc aaagtaaaaa agtatattct
     6541 gtaagagcta tttgttctac ttatcttcct gattggagaa aaggtactga ttatagaaat
     6601 aattattctg cctttaaatc tatgggggga ggagtagaat tagactgtat acatgaattg
     6661 gattatatta taaacctatt tggtttccct aaatcagtaa aaaaacagtt tggtaaatat
     6721 tcgaatcttg aaattaacag taatgattta gcaacatata tattgagata cgatgataaa
     6781 attatagaaa ttcatttaga ttattttgga agagttccta caagaaaatt tcaattgttt
     6841 actttagatg aatgtatcaa tgttgactta ttaaataata aaataagttt tgaagaaaac
     6901 attgttaaag aaatgcctga atctgctaat gatatgtatg agaaggagtt gaaatatttc
     6961 tttgaaaacg tagtaacagg caaagaaaac tggaatgatt tatatcatgc aaacgaagta
     7021 ttgaaaatag ctgaggagaa ataaatggaa attttattta ctatttgtgg tagagctggg
     7081 tctaaggggt ttaaaaataa aaatttgaaa tatttggcag gaaagcccct agtccatttc
     7141 gctttagcaa tgatagatat ctatcaacaa aaacatcctg aacataattg cacgacttgt
     7201 ttgaatacgg acagtaatga gttagtagaa ttgacgacta agttaaatcc gtctgtgaaa
     7261 attgtagaac gtaaacctga attagctaca gatactgtag ggaaaattga agtgatacgt
     7321 gattcattct tacaactaga aagagaagat aatatatttg atttaattat agatttagat
     7381 ttgacttcac ctttacgtga agtttctaat cttgaagaat taatagatga acatctaaaa
     7441 aatttagata aagacgtcta cttttcagtt gtttctgcaa gacgtaatcc ctattttaat
     7501 atggtaaaaa gaattgaaaa tgaagttact atagttaata aatcaagata cacatctagg
     7561 caacagacac ctgaagtttt tgaaatgaat gcctcaatgt attcttttaa acctgaattt
     7621 ctaaaaaaaa gtaattatat ttttgatggg caatgtggga taataaaaat gagagattat
     7681 ctaattttag atattgatag tgaagaagat tttaaatggt tagaattttt gtttcctaaa
     7741 tttttagaag attcttcgga gcttcaagat atctataatt atttatatcg gaggtaaaag
     7801 gatgagctgt tatcttatca ccggtggggc tggcttcatt ggttctacct tagccaatca
     7861 tttaggagaa gaacatgtgg taatcgtcgt cgatgactta tcaatgggaa aagtagaaaa
     7921 tcttgatatg acgaaaaata tcacttttat tgagggagat gtcgctgaga aatccttgat
     7981 ggaagagatc atgaaagcta ataaatttga ttatatcttt catttagcag cagttgctag
     8041 tgtggcagat tcagttgccc gaccagtaga aacacatcgt gtgaattttg aaagtgtatt
     8101 aatgatttta gaattgatcc gcaagtatca acctgattta aaacggatcg tgtttagttc
     8161 ttcagcggct gtttatgggg atgaaccgac tttaccaaaa aaagaagagt ccgtgattcg
     8221 cccattgacg ccttatgcgg ttgataaatt cgctgcagaa caatatgtgc tagattattg
     8281 tcatttatat gatgtacccg gtagcgcagt tcgttttttc aatgtctatg gtccaaatca
     8341 aaatccgaat tctccataca gtggtgtgat ttctattctc gtagatcgct ataagaaaca
     8401 aatcgtagga gaagcaactt catttacatt atttggtgat ggaagccagt ctagagactt
     8461 tgtttatata gatgatgtaa tccaagcatt acttttagtt gcgaatgagg aagctgcact
     8521 ggggaaacaa ttcaatgttg gaacgggtaa agcaaccaca cttttggaat tgattcatgc
     8581 aatcaatgaa attctcggtg tagaattgac acttgaatac aaagaagagc gttcgggtga
     8641 tatccatgac tctctagcag atattactaa gatccagtca attggctatc aaccgaaata
     8701 tggtatttta agtggaatga ctaattattt gaaaactgaa atcaattaaa acatactaag
     8761 ataagattag agggtaaatt caggctcttt gtcaataagg actgatgagt tttgtaaaat
     8821 caaatctggg ata
//
LOCUS       DAYYBG010000106         8679 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000106 DAYYBG010000000
VERSION     DAYYBG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.108"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            204..404
                     /locus_tag="ACHAUN_002449"
                     /pseudo
     CDS             204..404
                     /locus_tag="ACHAUN_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1116..1721)
                     /locus_tag="ACHAUN_002450"
     CDS             complement(1116..1721)
                     /locus_tag="ACHAUN_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8461116.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            1903..2238
                     /locus_tag="ACHAUN_002451"
                     /pseudo
     CDS             1903..2238
                     /locus_tag="ACHAUN_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2403..2618
                     /locus_tag="ACHAUN_002452"
                     /pseudo
     CDS             2403..2618
                     /locus_tag="ACHAUN_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2756..3727
                     /locus_tag="ACHAUN_002453"
     CDS             2756..3727
                     /locus_tag="ACHAUN_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8461117.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            3867..5180
                     /locus_tag="ACHAUN_002454"
     CDS             3867..5180
                     /locus_tag="ACHAUN_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFM8461118.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5223..6089
                     /locus_tag="ACHAUN_002455"
     CDS             5223..6089
                     /locus_tag="ACHAUN_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8461119.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6086..6934
                     /locus_tag="ACHAUN_002456"
     CDS             6086..6934
                     /locus_tag="ACHAUN_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8461120.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            6948..8420
                     /locus_tag="ACHAUN_002457"
     CDS             6948..8420
                     /locus_tag="ACHAUN_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8461121.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8437..>8679
                     /locus_tag="ACHAUN_002458"
     CDS             8437..>8679
                     /locus_tag="ACHAUN_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8461122.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2736 a         1489 c         1785 g         2669 t
ORIGIN      
        1 gagaacacca cgcatagttt ttattttgag atattctaga atgaaacaca ttaagaacga
       61 aagcaaagca aaagacgcca gaacttctac taccttctaa gttttttcat atttgtttgt
      121 tatgctatac atagatctgc ataattagac ttgcgctgcc cgttattgaa gtttcaggca
      181 gcgctctttt tgggggatcg ataatggaag aagaaaaaat atttgagaaa cgctggcagc
      241 tagcatcaag tgaacaaaga gcaagataca ataatttgat cacctcctac cccactgttg
      301 actagacata taaggaaaag aaatacttac tttggctgtg ccagttagat atagatacct
      361 ttgaaacatt tgaagtgatc ctagataaaa ttaaacagaa ttgatcagaa acaagcgaag
      421 ccttctctcg gttcgcttgt tttgctattt attagttggc tcagggcaac tctaagctcg
      481 tttgaaagaa aaccgatgga taatcccctt gtttgtacca ctttagaata catggatgta
      541 cgtattgcca ttgactccta ttagttactg aaaaaaaatt taaacaagtt gagtattgca
      601 gataaatagc tccagtgagg tgaataactg ctccactcaa ggcaatagat agctccatcc
      661 agggcaacaa actgctccac tttcatattt aatagatgaa taaccttctg tataatagaa
      721 aggcatacct ttcggtatga caacttatac agaatgttat tttattggtt cactatcgaa
      781 aaattttgga gctttatttt tccggtattt ctcaacgaac gattagttta actgtgggaa
      841 gctccagaca tacgattcga gaagttactt ctcgagcaga caggaagggg gtgactgaac
      901 taaccgctga tatgagcgat acttggctgt aggaatatct ttttccggaa aaacaaacaa
      961 cagcacgagg ttaccttcct gtagactggg aatatgttca taaagaacta atgaaaaaga
     1021 atatgacatt gaaactcctt catagagaat atggggtaca tggaacatag aaacgaaaat
     1081 gcacggtaga aaagcgaatg aatatagtat aaattctaca aatcattcgc tttctttatt
     1141 ttatatatgg ttggtcgaga gagaccattt tctttggcaa tttctgcaac tggaactcct
     1201 tgatttaaca tgtccaccac agttttaaag actaatcgtt tttgcggatc tgttgagttc
     1261 tcagaatatt caattggtcg ccccttatat cgaccagctt ttttggccaa taatactcct
     1321 tgccgctgac gttcacggat tttctcacgt tcgttttgtg caatataact tagcaatgat
     1381 aaaaacatat cgaacatagc agtatctagg agttcatttc cagtattaaa atctaaaaac
     1441 tgtgcatcta gtatttttaa tgctactttt ttctgtttca taaaggtaac tgtatttttt
     1501 atgtcttcat aatctctgcc taggcgatcc agtgatgcaa caatgacaca atcaccttct
     1561 cggatataat ttagtaattt ttgaaactca ggtcgctgga tatctttacc actggctttt
     1621 tcttggaata atttatcaat gttaaggtct tccatcattg ccaattgtcg atctaaattt
     1681 tgatcttgtg aagacactct aatatatcca atttttgcca taaaaactcc ttttaaaaaa
     1741 tgatataatt tacatgtaaa atttacttgt agttttactg ttatcatagc atttttaaaa
     1801 ataaatttta agtctactta taatttactt tggaggtttt gaatgaatga aattaagcgc
     1861 aataaaaatt cttttggtgt ttatttagga gagcctagct cggaacagtt aaataaaggt
     1921 gaagctcgtc atgctctatg tcgaaatata ttttatggta gaaaaggcaa actttatcaa
     1981 acttattacg acggtatgga ggaacagctc aacgcattag gattagtaac taatgctatc
     2041 atatattgga attcactata tttagaaaaa gtctttgaac aaattaaaac ggaagggttc
     2101 gactgttccg aagaagtgtt acgcaagcaa tttccattaa ttagtgatca cattaatttt
     2161 gttggcaaat atagtttccg ctatgaaaaa gaattatcga atggaatact acgtccgtta
     2221 aatatagaga attattgaga atgttcgtct tttggttcct accgtgcatt ttcgcacgga
     2281 tgttccagat actatttttg aatgtataaa attgaaaaca caagaatact aataaatcaa
     2341 cgattcttgt gtttttaatt tttcactacc gtgcattttc gtttctatgt tccatgtacc
     2401 ccaatcttga aaaattaatt aaagacttag ataaagccat tgacgactta gtcgtggtgc
     2461 taccggagta tcaatgttta accagtatcc caggtattgg tcctgtttag gctgctggaa
     2521 tactcgctga aattggtcca attgaacgtt tttctgatga aataaaattg acaaagtatg
     2581 ccggcctata ttggcgacag agtcaatccg gtaaatctga atacgaaaat ttttttacga
     2641 aaagcttcac gtctaatatc tattttaaaa taaaaatatt cctgctaatt tggaatcgtt
     2701 tccttctttt ggtataataa tacctgaaat tcttttgaga ggagttgttc gtgaagtgaa
     2761 aactactatg aaggatgttg ctgcgttagc aggagttggt gtgggtacgg tatctagagt
     2821 aataaatgga gtaaaggtga aagaggttac caaagaaaaa gtaatggctg caattagtga
     2881 gcttaattat gagcctgatg aatatgccag aggtatgaaa acgaatagta gcaatacaat
     2941 agctcttatt ttacctacga tttggcatcc cttcttttcc gagtttgcct actatgtaga
     3001 ggaagctctc agccaaaaaa attataagct ttttttgtgt aatgcgaacg gcagtgcgga
     3061 aaaagaaaaa gaatatatcc aaatggtcaa gcagagtaaa gtagacggaa ttataggtat
     3121 tacctattca gacattgata aatatgtatc atcaaattta ccatttgtca gtatagaccg
     3181 ccatttttcg gaacaggttt cttatgtaac ggcagataat tatttaggtg ggcaaattgc
     3241 cgctcaagaa ctacttactc gtaactgtaa aaacttgtgc tatgttgggg gtgtttcacc
     3301 ttaccctaac gaaacaaaca atcgtaaagc tggtttttac aatatttgtg aggaaaataa
     3361 aatagctgtg aaggtattgg atatgcctga accaattagc aatttagagg aacaattgct
     3421 cgcttttttt tcggataatc ctgaaattga tagcattttt accatcaatg atttcatggc
     3481 tttggatgtg ataaaagccc aaaaaaaact agggaaggaa gcacctaagg attttcaaat
     3541 tattggtttt gatggagtca agctatcgtt gcaaaaagac tatttactat caacgattgt
     3601 acagccgata gaggaaatgg ccaaggtttc agtagaaaca ctcatggcga tgattttaaa
     3661 tgaagaaccg cctaagcgtg tggtagtacc ggttttcttt cacgctggag agacgactaa
     3721 agagtaaaaa accggtttat aacgattttt ttgagatagg ttgacagaaa gtgatttcaa
     3781 tgatatctta aaaccaatta attggaaacg ttaccaatta attgaaaaat aaaacggaaa
     3841 cgtttcaaaa aataggagga acatcgatga aaaagagaat gttagcaatt gcagcaattt
     3901 tagcattaac agctggagga cttgcaggat gtggcagtaa ggagaaaagt tcaggtagca
     3961 gtgatccagg agaaattaag atttgggttc agttttctga tgaaacgcct gaaggaaaag
     4021 cttgggcaga gattgtgaac aattttaatg atgaatacga gggtgaatat aaagctgtta
     4081 ctgagtatat tcctcgtagc ggaagcggcg gtggatatga ggataaagta aatgctgcaa
     4141 ttacaaccaa tacccttcct gatgttatta cgttagatgg accgaatact gcagcttatg
     4201 ctcattcagg agtcattgct ccattagatg attatttgaa agatgcggac atggatgatg
     4261 tattagacag cattattcag caaggaacct acgatggcaa aatgtatgcg tttggtttct
     4321 cagagtccag tgtgggtgtc tattacaata agtcgctgtt caaagatgct ggaattacag
     4381 aagatcaatt gccgacgtta gaagatcctt ggacttggac ggaatttcaa gcaatttgcc
     4441 aaaagctgac tgataaatac aaaacgcctg caattgatat gcaaatggcg tcgggagacg
     4501 aaatgctgac ctacgcatat acaccattta tatggtcgaa tggcggcgat gtcgttaatg
     4561 ctgatggaac caaagccgaa gactatttta acggtaaaaa atccgttgaa gcgttggaat
     4621 ttattcaaga attggttgaa aaaggttaca ccacaatcag tcctattgaa aaaggatttg
     4681 aaactggcaa atatgccatg atgctgagcg gttcttggac aatggcagat ttagaggaaa
     4741 actatcaaga gattgattat ggtattttgc cttaccccgt ttcagatgca actggggagt
     4801 tagtttcacc aaccggaagc tggcagttag caatgactga gaaaacagag aaaaaggatg
     4861 cttctgcggc ctttattaag tttgcaacca gtacagaatc tagtcgtatt atgagtttag
     4921 ggaacagtgt tctacctatc agaaattcaa cagttgattt aattaaagat gaagtgtcgg
     4981 aaggaatgca ggtattaatt aagcaaaacc aactttctgg acatgcccgc ccagtggttg
     5041 tagcttatcc gcaagtatct agagcattcc aacaagcagt tcaagatatt agctacttcg
     5101 aggaaaaccc agatgtacaa aaagtagcag atagtcgcgc tgctgaaatg caacaggcaa
     5161 ttgatacttc actaaaatag aagtttctaa agggggccaa tcaagtgtta aaaaaaccga
     5221 caatgaaaga caatttgata ggttacggct ttctagcgcc agctttgctg ctgctgacga
     5281 tttttctgat catacccgta atcatggtaa tttattatgc ctttactgat tattatttgt
     5341 taacgccaga tgctagacaa tttatcggct tagaaaattt taagcgactg ttcgctgatc
     5401 caatttttgt aaaaagtatt tggaacacgg ctaaatttgt tgtttggatt attccggtcc
     5461 aaataggggc agctttagga atggcactta ttatcaacaa aaatcgtaaa ggaaatatct
     5521 tttttaaagt agcctttttt gcgccagtcg tgatgtcctt agtcgttatt tcgattttat
     5581 ggttgtactt gttaaatcct aatgaaggtc tggttaatac gctatttgca aagattggaa
     5641 ttgcttcgca gccttttttg actagtccca aacaagcaat gtatacgctt gttttcgtat
     5701 cggcatggca aggtgcagga tatcagatgc tgctgttttt agggggaatg caaaatattc
     5761 cgcaagacgt ttatgaggca gccgaaattg atggtttttc taagtttcag caatttcgct
     5821 atatcaccat gccattatta aagcccacgg cgatttttat tttacttacc acattgattt
     5881 cggcctttaa actaatcatt cagccaatgg taatgactca aggtggacca atgaattcta
     5941 ctatgacaat ggtttattac atttaccaaa ctggatttac agaccgtatg gtaggctact
     6001 ccagctccat tgcgttaatc tttaccacta tgattggttt aattacaatt gctcaaagaa
     6061 aattggtaaa ggaggatgat ccagcatgac caagaaattt ggaccgctaa tgatcgtaga
     6121 atattgtttg ctgttccttt tagcggcatt attcattttc ccgatgctgt ggatggtagt
     6181 ttcctctatg aagcctgaag cagatgtcta tcaaaattta gcttccttca aagctttttt
     6241 acctagtttg aatccagcta attggtttaa aacttatcaa gaggttattt cccggttcag
     6301 tgtgggaact tatttattaa acagtatttt ttatggttta acctttgcct gtgggtcaat
     6361 cattgtaaac tctttggcgg gttttgcctt tgcgaaggtt aatttttcag gcaagaaaat
     6421 tatctttgga gctttgttag ctctgctgat tgtcccgatg gagacggttt taattccgca
     6481 atttaccatc gttaattcac taggcttagt gaatactcgc ttggcagtga ttttaccagc
     6541 gatggccagc gcgtttaata tctatctgtt tagaaatttc tttatcgcga ttccagaaga
     6601 aatcattgaa tcagcgaaat tagatggtgc aaatattgtt aaaattttct ttcacatcat
     6661 gctgccaatg tctaaaccag ccatagccac agtaggtgtc ttatccttta tctccagctg
     6721 gaatgattat atttggccgt taatggtctt aactgacagc agcaaatttt caatgcaagt
     6781 ggcaattact acgatcaata ctacacagcc agtttatatc aatcaagtga tggctgtttt
     6841 aaccattgca acgattccac tgattattgt ttatatcgtt gcacaaaaat atattctgca
     6901 gggacttggc ggttctggaa cgggtataaa ataggagtga atagtgaatg ttgcaaaaac
     6961 ttttaaaggc caatgagttt attgagaaaa atgctcaaac agtcaatcaa gaataccgga
     7021 ataaatttca tttgatggct ccgattggct ggatgaatga tccaaatggt tttgtttatt
     7081 atcaagagga gtatcacttg ttttatcaat attatcctta cgacagcatc tggggaccaa
     7141 tgcattgggg ccatgctaaa agcaaggatc taattcactg ggagcatttg ccagtagcac
     7201 tggcaccagg tgaaagctat gacaaggaag gctgtttttc cggcagtgcc atagaaaagg
     7261 atggcaagct gtatttgatt tatacaggac acaatgttgt ggaagggcaa gttcgacaag
     7321 ttcagtgttt ggcagtttca gaagatggca ttcactttga aaaatatgcc ggaaatccaa
     7381 tcatcgcaga agatcactta gcttctgttg caacgaccga agatttccgc gatcccaaag
     7441 tgtttcagcg aaaagaagac tattttactg tagtcgctac taaaactgca gatgctcgtg
     7501 gacaaattgt gatgttccat tccaaagatt tactaacttg ggagttttat tccgtattgc
     7561 tggagggaac tccagaacaa ggcattgtat gggaatgccc agacttgttt gaactagatg
     7621 gcaaagaagt cttgattctt tctcctattc aaatgactcc acaaggcaat gcttaccaaa
     7681 atacgaactc aactgttgct ttcatcggta ctgtcgattg gcaaacaggc aaatttctag
     7741 tagagaattt tcatgaaatc gatggcggtt tagattttta tgcaccccag acctgtgaaa
     7801 acgaagcagg ccaaagaata atgattgctt ggatgcaaat gtggcagcgt aaaataccta
     7861 cgaatgatct gggccatggt tggtcaggtt ccatgacctt gcctagagag ctgagggtgg
     7921 ctgataatca attgttgcaa cggccagttt catcaattta tcaagtaatt gatccgcagg
     7981 aaaggattga atgggcgaag actgaagaga atcgcttctt actgaaggac ttagtatatg
     8041 accagcacta tttacgcttg gaaatcaatt taaaacaggc aacgaaacta gaacttcact
     8101 atgcgaaggg acccgaccat acattactgc ttaactatga tgttgaacgg gagtgcttta
     8161 ctgcttccag ggaacacatg ccgtatgatt tatcaggagc tgaaaaggaa gtgttgaaaa
     8221 agcgcagtgt gacgattcct ttggaaaata attgtctaac catcgaaatt ttccgtgata
     8281 cttcagctat tgagcttttt ctaaatgatc gtctgactat gagtacaact ttttatgaga
     8341 cagaaaaagg acaggatttt cttttgctgg cagacagtga agtaaccttt tcattggaaa
     8401 gcggcttaat taatctatag aagggagagc tcagtgatga tttatggttc aatcgaagca
     8461 ggcggcacaa aatttgtgtg tgccattagc gatgaagaaa tgacgatcaa ggaaagagtg
     8521 agctttccga caacgacacc tgaggaaaca atacctttag tcattgactt cttcaaacaa
     8581 taccaagcag atttagcagg gattgggatt ggctcatttg ggcctatcga tatccatcgc
     8641 gattccgcca cttatgggta cattacctcg accccgaaa
//
LOCUS       DAYYBG010000107         8546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000107 DAYYBG010000000
VERSION     DAYYBG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.109"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..492
                     /locus_tag="ACHAUN_002459"
     CDS             <1..492
                     /locus_tag="ACHAUN_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8461123.1"
                     /translation="RGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTS
                     MIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVS
                     LTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTR
                     KED"
     gene            585..1747
                     /locus_tag="ACHAUN_002460"
     CDS             join(585..861,861..1747)
                     /locus_tag="ACHAUN_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8461124.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2027..3007
                     /locus_tag="ACHAUN_002461"
     CDS             2027..3007
                     /locus_tag="ACHAUN_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8461125.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3129..4547
                     /locus_tag="ACHAUN_002462"
     CDS             3129..4547
                     /locus_tag="ACHAUN_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8461126.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4618..6087
                     /locus_tag="ACHAUN_002463"
     CDS             4618..6087
                     /locus_tag="ACHAUN_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8461127.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6097..6564
                     /locus_tag="ACHAUN_002464"
     CDS             6097..6564
                     /locus_tag="ACHAUN_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8461128.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6600..7472
                     /gene="scrK"
                     /locus_tag="ACHAUN_002465"
     CDS             6600..7472
                     /gene="scrK"
                     /locus_tag="ACHAUN_002465"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8461129.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7554..>8546
                     /locus_tag="ACHAUN_002466"
     CDS             7554..>8546
                     /locus_tag="ACHAUN_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8461130.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSG"
BASE COUNT         2944 a         1287 c         1739 g         2576 t
ORIGIN      
        1 agaggtgttt atgtggttga taaaattcag tttaatcaag ttatagaaga attcatcaaa
       61 gtaaataatt taatgaccca tattcaaaaa cgaccaatca gaataactaa tgatctaaaa
      121 ctcagtacga gtatgattca tttgattgat gtgattggaa attatcctga aactagtatt
      181 acggaattag ctgatcgatt aggcgttacc aaaggagctg tatcccaaca gattcccact
      241 ttagagaaaa tgaatcttat aagcatttct caaaaaaaag gaaataagaa aactaaactt
      301 gtatcgttaa caaaatatgg tcgaaaggtg tacgaatcac ataattattt acatgaagag
      361 ctttatagct caataaatga agcgttagaa tcattctcac cagaccaaat ttcaaccatt
      421 aaacagattc tagaaaaagt atcttataca atcaatgaat atcaagtaag tctgacaaga
      481 aaggaagatt gacatgaaaa aaatgatttt tgaatcggct acactagact agacagaaaa
      541 aataaggtgt gtaaaataaa tgaaaacaca ctggaggaat ttttatgtca agacgtcaac
      601 gtagaaccta ttcaaaagaa ttcaaacaac aagtcgtcga tctttatctc gcaggaaagc
      661 cccgagctga aatcattcga gattatgagc tgactacctc ggcctttgat aagtgggtca
      721 agcaagcgca aactactggc tcgtttcagg aaaaagataa cttgacccct gaacaagtag
      781 aactaattgc attacgaaag aaaaataaac agcttgaaat ggagaatgac attttaaagc
      841 aagcggcgct gatattggga cgaaaagaca agtaattgat accaataagc acaaatactc
      901 aatatcagcg atgtgtaaag tgttaaacat ctcttgccaa acctattatt atcaagccaa
      961 accagttgaa gatgaatcag aactggaaga actcgttcaa gaagaattta ttcgcaatcg
     1021 aaaagcttat ggtaccagga agctgaagca gtgcttggcc aatctaggtc ttcaagtgag
     1081 tcaacgcaga atcggccgta tgatgaaaca gcgtggcttg cgttcaacat acacattggc
     1141 tcatttcaaa ttccattctt ctacttgtaa cgaagccaag accgccaata ttctagacgg
     1201 aaccttccta caggaaaagt cattagaagc gattgtaacc gaccttacct atgttcgtgt
     1261 tgggaagaaa tggcagtata tctgcctgat tcttgatttg tttaatcgag aaatcatcgg
     1321 ctattcatgc ggggagaaga aagatgcgac gttagtcaag gaagcttttg caaagattcc
     1381 gtactcgttg aataatgtac aactttttca cacggatcgt ggaaaagaat tcgataacca
     1441 gacgattgac gaaattttat gggcctttaa tatcacgcgt tccttaagca aaaaaggctg
     1501 cccctatgat aacgccgttg cagaatccac ctataaatct gtcaaagtag aattcgttca
     1561 tcaataccgc tttgagacat tggctaattt gcgattagag ctatttgatt acgtccattg
     1621 gtggaatacc atccggctgc acggcacgtt aggctatgag acaccgatca gttttcgaca
     1681 gcagagattg gcgcagcgaa tccttgataa tgagctcgga tgtgataccg gtggcgaggc
     1741 accttaatta tttgaatgag cttctgcctt agagaatcag attcgaggag gctcgttgtc
     1801 aagggcagtc ggaacaccag cagcaagctt acgcaatacc ttataatttt tgtcaaaaaa
     1861 agtgttgcca ttccacatat atctttaaac agctattgcc agataaaaat aaatgatatt
     1921 atatatataa atacttatcc gaaaagggga atgagagagt caaggttctt ggaatgtcga
     1981 atagtcgagt aggtagattt tctatctaca accttgttgt ttaaaaatga aaaaaattgg
     2041 aattagagaa gttgcgaaag aggcaaatgt ttctcctacg actgtgtcga gggtattgaa
     2101 tgatcgtgga tatttgagtg aggaaacaaa aagaaaagtt tttgatgcca tgaaaaaatt
     2161 agaatactat cctaatgaac tggcgagagc actttttaaa aacgaaacat ataccgtagg
     2221 tttgattttt cctacaatta ctaatccatt tcacgctgag ttaattcaag atatcgagtt
     2281 cgagctttcc agcaggggat ataaagtgtt gctgtgcaat agcttaaaca atccaacaaa
     2341 agaaaaagag tacttgacga tgttgagaaa gaatcaggtt gatggaatta tagtaggaac
     2401 gcacaataaa gatattgcgg attatgatat tccagggcta cctattgtag cgattgatag
     2461 aagtcttggt gaaaatacag ttacagtatc ttgtgataat tacaggggtg gggaattagc
     2521 tactcaacta cttgtaaatc atggctgtca gaatatactg tgtattcggg gagatagtaa
     2581 gataaagcta cccgcgaata gtaggacttt agcatataaa gatgttttga ataaattcat
     2641 gctggaaccg ttagtcctag aagtaccatt tgtaaaagaa atcaatgaaa aaaatgaaat
     2701 aattaagaca tatctggaaa ctcatccaaa tattgacggg gtttttgcag gagatgattt
     2761 attggcttct ttggcgatta acaacttagg aagttcgggc aaaagagttc caaaagaggt
     2821 taaagtgatt ggatttgatg gagctcaaca aacattgatt tataatccag aattatcaac
     2881 tattcaacag cctatagatc aaatatctaa agtagcagta aaaaaattat tgaatatgat
     2941 taagggcgaa aaggagacgg atgaactttc acttccagta aagctcataa aaaaaaatac
     3001 agcataagtt aaatcggcga aaaattcctt cgtcgattta aaaatatttt tatgaaacgg
     3061 gttgacatat gaaacgggtt gacatatatt agttgtgaag ataaaaacct tttcactgga
     3121 ggaagaaaat ggagaaatta ttaattaaaa gcaataatca tcaactttac taccaaccta
     3181 aggatgtttg ggtcggagat gttatgcctt acgggaaaga aggaaaattc tttatttatc
     3241 atcaacgtga tactagaaat ccagttcctt ttggagaacc gtttggctgg gctttagcaa
     3301 ctacgaaaga ttttgttcat tttgaagact ttggggaatc tcttttaaga ggggatgaca
     3361 atgagagaga ccagtttatc tacgctggtt ctgtatttga ggcaaatgga aaggttcacg
     3421 cattttatac agggtataat cgtgaattct tagcgcaggg aaagacatct caagtgttgc
     3481 ttcatgctga gagtgatgat tttgttcatt gggaaaaatc agaaacagca ttggagttag
     3541 aaccacaaga agggtatgat attcgaaact ggagagatcc atttgtcgtt tgggatgatg
     3601 aaaaagaaga atatttgcta attttagggg cgcgaaaggg tcaggataaa catctacaaa
     3661 ccggacgttt agtggaattt acatcaaaag atttaactaa ttgggaattc aaaggtgatt
     3721 tttgggctcc gggattgtat accatgtttg aaatgccaga acttttcaag attggtgact
     3781 ggtggtatct tgtctattca gaatactcag atggaaataa aatactctat cgaatgagtc
     3841 aaagtttgga aggtccttgg attaagccta aagatgaagc ttttgatgga cgggcttatt
     3901 atgcaggtag aacagcttat gatggaaaac gtcgagtcct atttggatgg gtaccaacaa
     3961 aagatcaaga aaatgataaa aacaattatt tatggggagg tactttggtt cctcatgaag
     4021 tctatcaaaa agaggatttt tcacttgggg taaaacccat agacgaagtg tggaatgcat
     4081 tcataaattt acatgatatt gccgatgtag agatagaaac agcagacaca agagaagaag
     4141 taacgcttgt caaaaatagt ggaactttgt ttaatttaga aacaacaatt tcatttgatg
     4201 agaatcttag tgacttttca ctgagattat ataaaaacct tgagacagat gaatcttacg
     4261 aatatcgctt caatttagat gaggggcgtc ttacatttga taaaaaccct tgttaccctt
     4321 ggtttcaata tatggataaa ggacttatta gacctatatc tttaaagcct aaccaatctt
     4381 ataaactaaa agtgattgtt gatgacaata taatgacact ttatattgat ggaatcgctc
     4441 ttaacgctcg tgtacatggt tgttttggtc aagcaattac tattacagca acaaatggat
     4501 caatgactgc aataaatacc aagttttcaa atgaaataaa gaaataataa acgtcaatca
     4561 tgtggcaggt ctgaaaatca aacatgacct gtcacattta aaagggagga taaatgaatg
     4621 gactacaaaa aaattgccga agatgtattg gaaaacgttg gaggaaaaga gaatatctca
     4681 agcgctacac actgcattac tcgtttacgt ttaattctta aagacaaaag caaagcgaat
     4741 atggaaatgc ttgaggaaat agaaggtgca aaaggtgttc ttttcaatag tggacagtta
     4801 caagtaattt tcggaacagg tataattgaa aaggtttaca aagagttcgt tgaattgaca
     4861 ggagctaagg aagtatccgt ttctgaaatg aaagacgaag gagtcaataa ttcaggaaat
     4921 agacttcaac gagcttttaa agttttctcg gatatcttta ttccgatcat tccagctttt
     4981 attggggcag caatggtttt aggaattcgt tcgttgatta ccactcctgg aatgtttgga
     5041 atggatggct cattcgctga tcacaatgta ttcctaaatg atttatccaa atttctgggt
     5101 attattgcaa ccacatttga ttatttacca gttttagtta tgtattctgc aacaaaacgt
     5161 tttggcggaa accctatttt aggattactt gtaggatttg ttatgattca tccaggacta
     5221 gccaatcgga atgatttcgt catgggaact ataaagcctg attattggca tttatttgga
     5281 ttaagcattc cagcagttgc gtttcaaggg ggtgtttttc cagcaatttt gacttcttgg
     5341 tttttagcaa aagttgagaa aattacaaat aaatattctc ctcaagtact tgcttacatt
     5401 ttagtcccaa ctgtgacaat tgttcttgcc aactttgcac tatttttagt gtttggtcca
     5461 ttggggaatt ttatcggtaa tgttttaggt ggaatcattg atttcctata tatgaagatg
     5521 ggtgcctttg gtgcttttgt ttttgcaggc gcgttacaac cattagttgt aacaggaaca
     5581 caacatgcta ttcaaggtat tgaggcaaac ttagtcgcaa caactggatt caattatatc
     5641 caacctattt ggtcagtttc aattattgcc caaggtggcg gttgtattgg tatgtattta
     5701 ctctttaaga aaaaatctaa agatagagat attgcgattt caagctttat tccaacactg
     5761 gtaggtatta ctgaaccggc tatctttgct gttaatttga aatattcaat cgttcctttt
     5821 gtttgctcgt ttattggtgc tggttttggt ggagcattta tgaaaatttt tgatgtaaaa
     5881 gccattggac aaggattgac tgtattgcca ggtctgacta ttgctaatcc agttcttcct
     5941 tatatatttg gtaatctcat agcattcatt ttaccaatca tttttattct cgcttatgat
     6001 aaatataaag tgtttatccc tgaagctaaa caccgtaaat ttgcagaaaa aactgactca
     6061 aaaaaattgc cagttcaagt aaagtgagat gagagcatgt taaatttttt tagaaaaaat
     6121 gaaataagcg ttgtagcacc tgcaaccggt gtttttgaaa aactggagaa attaagtgat
     6181 ccagtctttt caaaaggaat gatgggacaa ggatttgcaa ttgatgcaaa aaacgaaacg
     6241 attgtatctc caatcgatgg ttttgtttca agtatttttc ctacaaaaca tgccttagga
     6301 ataaaaacaa aatcaggaat tgagatactt gttcatgtag gaatagatac agttgagcta
     6361 aatggtgagg gatttgatat taaagttcaa gaaggacaaa aagttaaggc tggggatatt
     6421 cttatgacca ttaattttgt agtttttgaa aagaatcaca agactaaaga tgtcattgtt
     6481 gtttttccag actaccaaga cgaaattttc attccgaaac ttgaagaagt aaaacaagga
     6541 caagaattgt ttgttcttgg ctaatgataa gtaattaata atagaatcga ggaaaatgta
     6601 tggtttttgg ggctattgaa gctggtggaa caaaatttgt ttgtgccatt ggagataaag
     6661 atttgaacat atttgaaaaa gtcagctttc ccacgactac acccaaagaa acaatgaatt
     6721 tagttttaga tttttttaat catcataaag atgatttaga aggtatcgga gtgggatcgt
     6781 tcggtcctat tgatattcat tgtgaatcta aaacgtatgg cttcattaca tcaacgccaa
     6841 aacttgcttg gcaggatttt gattttattg gaagtttaaa aaagcaactt aatattccaa
     6901 ctgcttggac gactgatgta aatgcctctt gctatggtga atatgtttct ggaaatggca
     6961 gaggcctttc aagtgttgtt tactataccg taggaacggg cataggaggt ggcgctatcc
     7021 aaaacggaaa cttcattgaa ggttttagtc atccagaaat gggacatatg cttgtaaaac
     7081 gacacaataa agacaacttt tctgggacat gtccattcca cggagactgt ttagagggct
     7141 tagctgctgg tccggcaatc gaaagaagat cagggaaaaa gggacaagaa gtgactgaaa
     7201 atgacgttac ttgggaaata gaagcttatt acattgctca gtgtgcatat aatacaacat
     7261 tgatgttatc acctgatgtc attatatttg gtggcggagt aatgaaacaa aaacacttaa
     7321 aaaagaaagt acaagataag tttattgagt tgatcaacgg atatgtcgct attcccgatg
     7381 ttgacaatta tattatgaca ccaaaattgt tagataattc tggtattgta ggttgtttag
     7441 ctttagcaag agacgttaaa gaaaaatctt aatttggaaa aaagatcgtc tcgactattt
     7501 gtttggttga tgcgatcttt tttttcaatt agtaattgaa gggacgccta aaagtgaaaa
     7561 aaaaaatgaa agatgaagta ttagagtata tcaaaaataa tttaaagtat tacgattttt
     7621 ctgctcaaga tattgcaatg aaattttgta ttaaaagaaa tgtcgcttcc cactatttga
     7681 atcagttatt ttcagatgga aaattactaa agaatgattc tgttagacca gtaatgttca
     7741 agtataatca acaaaagcca aaagactgct tttctaagtt tattggagca gacatatctt
     7801 tgaaatccac tatagataaa tgcaaagcta ctgtaatgta tccccctaat ggcctcccat
     7861 taataataaa aggaaacagt ggcgttggaa aaagtttttt agcaagtttg atctatcagt
     7921 atgctcttga tagaaaagta attcataatg acgcaaaatt tgttgttgtg aattgtgcag
     7981 attatgcaaa taatccagag ctattgtcag cagttttatt tggatataaa aaaggagcct
     8041 ttactggggc tgaaaaagat actgcaggga tgctatcttc tgctaatgga ggataccttt
     8101 ttttagatga ggttcacaat ctaagtgcag aaaaccaaga gaaattgttt cttcttatgg
     8161 attctcaaaa gtatcgtaaa cttggtgaaa gtgtgcaatg ggagtatgca aatgttcgcc
     8221 ttatcttagc cacaacagaa gataaaaata cctctttgtt agcaactttt agaagacgca
     8281 tcccagctga aatcactctg ccagattaca ataatagaag tacatctgaa aaaattcaat
     8341 tattatttga atttcttaaa gatgaagcaa ttacaattga ttcaaaaata ttttgttcga
     8401 ttgaactttt tactgatctt ttatcgaaaa catttgaagg aaatgttgga gaattgagga
     8461 atgaaatcaa gcttctttgt gcagaaggtt atttaggaaa taagagaaag tcttcaattt
     8521 atttagggga caaactagac agcggt
//
LOCUS       DAYYBG010000108         8494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000108 DAYYBG010000000
VERSION     DAYYBG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.110"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHAUN_002467"
     CDS             complement(<1..149)
                     /locus_tag="ACHAUN_002467"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8461131.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHAUN_002468"
     CDS             complement(593..1714)
                     /locus_tag="ACHAUN_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM8461132.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHAUN_002469"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHAUN_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM8461133.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHAUN_002470"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHAUN_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM8461134.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHAUN_002471"
     CDS             complement(4304..5215)
                     /locus_tag="ACHAUN_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8461135.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHAUN_002472"
     CDS             complement(5232..6239)
                     /locus_tag="ACHAUN_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8461136.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHAUN_002473"
     CDS             complement(6236..8365)
                     /locus_tag="ACHAUN_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8461137.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACHAUN_002474"
     CDS             complement(8370..8480)
                     /locus_tag="ACHAUN_002474"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461138.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAYYBG010000109         8331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000109 DAYYBG010000000
VERSION     DAYYBG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.111"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..466)
                     /locus_tag="ACHAUN_002475"
     CDS             complement(<1..466)
                     /locus_tag="ACHAUN_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8461139.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANF"
     gene            complement(596..1306)
                     /gene="budA"
                     /locus_tag="ACHAUN_002476"
     CDS             complement(596..1306)
                     /gene="budA"
                     /locus_tag="ACHAUN_002476"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8461140.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1319..2983)
                     /gene="alsS"
                     /locus_tag="ACHAUN_002477"
     CDS             complement(1319..2983)
                     /gene="alsS"
                     /locus_tag="ACHAUN_002477"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8461141.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3188..3850)
                     /locus_tag="ACHAUN_002478"
     CDS             complement(3188..3850)
                     /locus_tag="ACHAUN_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8461142.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3860..4675)
                     /locus_tag="ACHAUN_002479"
     CDS             complement(3860..4675)
                     /locus_tag="ACHAUN_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8461143.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4937..5380)
                     /locus_tag="ACHAUN_002480"
     CDS             complement(4937..5380)
                     /locus_tag="ACHAUN_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM8461144.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5514..5852)
                     /locus_tag="ACHAUN_002481"
     CDS             complement(5514..5852)
                     /locus_tag="ACHAUN_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8461145.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5840..6217)
                     /locus_tag="ACHAUN_002482"
     CDS             complement(5840..6217)
                     /locus_tag="ACHAUN_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8461146.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6441..7634
                     /locus_tag="ACHAUN_002483"
     CDS             6441..7634
                     /locus_tag="ACHAUN_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8461147.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7800..8222)
                     /locus_tag="ACHAUN_002484"
     CDS             complement(7800..8222)
                     /locus_tag="ACHAUN_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461148.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2444 a         1692 c         1407 g         2788 t
ORIGIN      
        1 taaaatttgc taataaaaga tctacatatt tttctagaaa tgcttgatcg atttcatcat
       61 accctttcgt tactccgcaa tcgatatcta aaacaccgat taaattacct tcttttacca
      121 ttggcactac gatttctgat tgtgcggctg aatcgcagga aatgtaattt gcatgggttt
      181 tgacatcatc aacgatcaat gtttcttgtt tttcagctga ttctccacaa actccttttc
      241 ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg tcctaaaatc aattcattac
      301 catcgaataa ataatatcct gcaaagacag tctcaggcaa agtctcattc aacaatgcag
      361 atgaatttgc taaatttgct aaccagttat cttctgcttc taataatcct tgttgctgta
      421 ataataaaat ttcgtatgca tcttttttct gtttttctgt ccacatgata aaaaccgctc
      481 ctttttgatc tatggagaag attatagctc agtttttttg taaacgctag gtttcctcct
      541 caaaaaaacg agatacgtca ggttaatcac cttgaacgca tctcgctgtc tttttctatt
      601 ctgcttgttg gatttctgct gctaaatcag cataatcaat cgtttcgtga acaaaagtcg
      661 gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg gctaatctca acttttcctt
      721 cagccacttc aaaatccatt acatgtccgc caaatgtatg tgtatcatcg ataaaatgca
      781 agtgaaatcc cgctgcagca actccttgga ataattcagg cgtaaagaac ccgactaatg
      841 ttccttggac attgctttgt tggaattctg gttgagactc tgaaattttt gctagtcttc
      901 tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat atttttgaat aatccagtta
      961 tcttcactgc caaaaatagg ttttccccgg cttctcttac taatatgtct tttttcaatt
     1021 gttctccatc tgcagttttt gagacagaaa aactagtatc tggcgtaaaa tctgtaattg
     1081 ctgcataggg ggtaagttca cttccatcta attgggcaac tgagccatct gatcttcctt
     1141 gataagctac gccatctaaa aagatgactt caccatcaag cccatgcaat gttcccaatc
     1201 ccagagttcc tttttctaat atatgagcaa tcgtttctgt tccatccatc aagccagcca
     1261 tcaaagctcc taatgtcccg tgctggtata agattttttc agtcatgtcg acacttcctt
     1321 aatagaattg atctggtaag attgtttctc ccaatttctc attatcacgg taatcaatcg
     1381 gaatatcaat aattacgggt ccttctgtcg caaatccttc ttctaaagct ttttctaatt
     1441 cctctacact tgtagctcgg attcctttag cgccaaaagc ttctgcatat ttcacaaaat
     1501 caactggacc aaagtctaca cctgacgccc gctgatattt cattttttct tggaactcta
     1561 ccatattgta gtgtccatca ttccaaatca aatgaacgat attcaatttt ttacgtaccg
     1621 ccgtttctaa atcctgggca gagaataaaa atcccccatc gccggaaact gatacgattt
     1681 gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt
     1741 gcataccatt actgaataac aaatgacgag gttcataact tctaaagtgg cgtgccatcc
     1801 aaatataatg actacctaca tctacggtaa cagtcatatc atctgttact tttgactgaa
     1861 gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc tccttttgat tgaacaatgt
     1921 cccgctccgt taattctgct tgaagagatg ctaaatattc ttttgcatct tctgacactt
     1981 gttgaggctc taatgaacct gtcaataaat caagagtggc tgagatatca ccgatcaatt
     2041 ctctttcagg ctgcataaaa ggatcgattt cagctggcgc ttcatcgatc acaatgatcc
     2101 gcgcatcttt ttccgcattc cagtttcttg cttcatattc gattggatca tagccaatag
     2161 caataactag atcacttcgt ttcaaaagca tatctcctgg ttggtttcta aataaaccca
     2221 cacgaccaaa gaaatgcgct tctaattcac gtgagatcac tccagctgct tggaatgtct
     2281 ctacaacagg caatgctgtc tttccaacta attgcctgat ggctaacgtt tctttttcac
     2341 ttgatcctcg catacctact aatagtaccg gtagttttgc ttctctaatg cgttcagcta
     2401 ggtaacggat atcatctgca gaagcagaac ctaatttagg atcgctcaat ggcttgatga
     2461 cattcccttg gacaggtgca tccactacat cttgcgggat actgataaag ctcgcgcctt
     2521 tttttgaact cttagccata cgataagcat tggcaatcac ttctgaaatt gtttctggat
     2581 cttggatttc tgcactgtat tttgtgattg gctggaacag tgctgcatta tccatgcttt
     2641 gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt
     2701 caccttctgc tgtcgctgtt accaaacctg tagctaagtt actagcacca ggaccacttg
     2761 ttgcgataac tactcctggc tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag
     2821 cattttgttc gtgacgagtc acgattaatt caggaccttg atcctctaat tcattaaata
     2881 caccatcgat ttttgcacct ggtataccga atacataatc aacatgatga ttaatcaaac
     2941 tttctactac agatttcgaa ccttgtttca ttttttcgtc cattgaacat cgtccttttt
     3001 atgctttatt tttaattatt agttagaaat aatcacaaat aattgataca gtttctaaac
     3061 ctgtttcatt caaaagtata gcacgtttta tttcatttgt ttagccatat gtttcaaaaa
     3121 atgaaaaaga atgacagttt ttacataaaa actgtcattc ttaattgaaa gtttcaattt
     3181 ataaagtcta cagatccata gttctcatca atttacgatt gttgaagtaa atcaaaccaa
     3241 atacgataga aggacctagt agaatacacc attggggctt tacgaaaaag aagagaaaca
     3301 aacttgtcaa tagtaaaaaa tctagtaaac tcacatacca ttttttcaac gtatagtaga
     3361 acgctatccg tagaacttga tttggtttcg acgctggatt tctcacctga aaataccagt
     3421 agttgatgct aaaggaaact gataaacaag caagtaaaac aaacaacgga atcaaccatt
     3481 ttccataaga aaaacgtata aaaaagaaac aatcaaaaat cattatcaca ctaactatcc
     3541 aagcaaatag ccaataaaaa aatcccttta gccaaaattg gcgaaacgtc tttatgaaca
     3601 aacgtactgg tgaaaaactt ttttcttttg ctaattcttc tgtcatacct atcatcacga
     3661 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
     3721 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
     3781 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
     3841 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
     3901 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
     3961 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
     4021 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
     4081 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
     4141 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
     4201 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
     4261 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
     4321 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
     4381 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
     4441 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
     4501 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
     4561 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
     4621 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     4681 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     4741 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     4801 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     4861 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     4921 caattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     4981 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     5041 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     5101 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     5161 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     5221 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     5281 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     5341 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     5401 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     5461 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     5521 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     5581 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     5641 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     5701 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     5761 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     5821 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     5881 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     5941 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     6001 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     6061 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     6121 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     6181 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     6241 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     6301 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     6361 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     6421 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     6481 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     6541 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     6601 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     6661 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     6721 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     6781 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     6841 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     6901 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     6961 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     7021 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     7081 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     7141 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     7201 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     7261 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     7321 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     7381 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     7441 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     7501 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     7561 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     7621 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     7681 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     7741 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     7801 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     7861 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     7921 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     7981 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     8041 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     8101 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     8161 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     8221 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     8281 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc t
//
LOCUS       DAYYBG010000110         8310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000110 DAYYBG010000000
VERSION     DAYYBG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.112"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHAUN_002485"
     CDS             476..1339
                     /locus_tag="ACHAUN_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8461149.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHAUN_002486"
     CDS             complement(1468..3090)
                     /locus_tag="ACHAUN_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8461150.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHAUN_002487"
     CDS             3339..3860
                     /locus_tag="ACHAUN_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8461151.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHAUN_002488"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHAUN_002488"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8461152.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHAUN_002489"
     CDS             5941..6759
                     /locus_tag="ACHAUN_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8461153.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHAUN_002490"
     CDS             complement(6862..7059)
                     /locus_tag="ACHAUN_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461154.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHAUN_002491"
     CDS             7228..7806
                     /locus_tag="ACHAUN_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8461155.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHAUN_002492"
     CDS             7901..8038
                     /locus_tag="ACHAUN_002492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461156.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2504 a         1489 c         1638 g         2679 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacat cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg
//
LOCUS       DAYYBG010000111         8129 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000111 DAYYBG010000000
VERSION     DAYYBG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.113"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACHAUN_002493"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACHAUN_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACHAUN_002494"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACHAUN_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACHAUN_002495"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACHAUN_002495"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8461157.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACHAUN_002496"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACHAUN_002496"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8461158.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACHAUN_002497"
     CDS             5704..6489
                     /locus_tag="ACHAUN_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8461159.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACHAUN_002498"
     CDS             6502..7353
                     /locus_tag="ACHAUN_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8461160.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACHAUN_002499"
     CDS             complement(7477..7929)
                     /locus_tag="ACHAUN_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461161.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         2736 a         1380 c         1719 g         2294 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatga
//
LOCUS       DAYYBG010000112         8023 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000112 DAYYBG010000000
VERSION     DAYYBG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.114"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..1797
                     /locus_tag="ACHAUN_002500"
     CDS             259..1797
                     /locus_tag="ACHAUN_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8461162.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1794..2759
                     /locus_tag="ACHAUN_002501"
     CDS             1794..2759
                     /locus_tag="ACHAUN_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8461163.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2746..4068
                     /locus_tag="ACHAUN_002502"
     CDS             2746..4068
                     /locus_tag="ACHAUN_002502"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8461164.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4131..4235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4309..5052
                     /locus_tag="ACHAUN_002503"
     CDS             4309..5052
                     /locus_tag="ACHAUN_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8461165.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5067..6059
                     /locus_tag="ACHAUN_002504"
     CDS             5067..6059
                     /locus_tag="ACHAUN_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8461166.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6071..6808
                     /locus_tag="ACHAUN_002505"
     CDS             6071..6808
                     /locus_tag="ACHAUN_002505"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8461167.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6798..7520
                     /locus_tag="ACHAUN_002506"
     CDS             6798..7520
                     /locus_tag="ACHAUN_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8461168.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7604..>8023)
                     /locus_tag="ACHAUN_002507"
     CDS             complement(7604..>8023)
                     /locus_tag="ACHAUN_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8461169.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2570 a         1389 c         1751 g         2313 t
ORIGIN      
        1 aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag
       61 atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct
      121 tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt
      181 aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat
      241 aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag
      301 ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca
      361 ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta
      421 ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc
      481 ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt
      541 ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca
      601 gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca
      661 ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact
      721 ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga
      781 aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc
      841 agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca
      901 gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa
      961 accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa
     1021 gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg
     1081 agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt
     1141 actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa
     1201 gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta
     1261 aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa
     1321 catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc
     1381 acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg
     1441 aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga
     1501 gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg
     1561 actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa
     1621 gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata
     1681 acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca
     1741 gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta
     1801 aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc
     1861 taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa
     1921 aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact
     1981 tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg
     2041 tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc
     2101 ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa
     2161 aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag
     2221 aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg
     2281 acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt
     2341 ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa
     2401 tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac
     2461 cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga
     2521 aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa
     2581 taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc
     2641 ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga
     2701 caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat
     2761 gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca
     2821 ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga
     2881 tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg
     2941 agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa
     3001 cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc
     3061 gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa
     3121 gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg
     3181 gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa
     3241 gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct
     3301 gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg
     3361 tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt
     3421 gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg
     3481 gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta
     3541 gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg
     3601 caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa
     3661 ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca
     3721 agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct
     3781 gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt
     3841 accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt
     3901 gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc
     3961 gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc
     4021 atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa
     4081 ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc
     4141 tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag
     4201 ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc
     4261 atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat
     4321 ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt
     4381 tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca
     4441 gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg
     4501 gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta
     4561 gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg
     4621 ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg
     4681 ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag
     4741 caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa
     4801 ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc
     4861 agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata
     4921 cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta
     4981 ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt
     5041 tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt
     5101 tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt
     5161 tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc
     5221 tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag
     5281 gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt
     5341 atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg
     5401 ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt aacatccatt
     5461 caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg
     5521 tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa
     5581 gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag
     5641 gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag
     5701 aattagatga acggttagat tactatactc cggtaattat tacgaatgat caactgatca
     5761 aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag
     5821 ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc
     5881 ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga
     5941 attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg
     6001 gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg
     6061 agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat
     6121 attagaagac atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag
     6181 cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg
     6241 aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc
     6301 gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa
     6361 aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt
     6421 aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc
     6481 cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc
     6541 gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc
     6601 aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag
     6661 gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa
     6721 acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt
     6781 attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt
     6841 tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag
     6901 aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc
     6961 atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa
     7021 ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa
     7081 aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc
     7141 acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt
     7201 tgatccattg gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt
     7261 tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc
     7321 caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg
     7381 ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg
     7441 agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt
     7501 tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg
     7561 ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg
     7621 cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg
     7681 ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt
     7741 aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga
     7801 tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag
     7861 caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa
     7921 caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt
     7981 gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAYYBG010000113         7856 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000113 DAYYBG010000000
VERSION     DAYYBG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.116"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..787
                     /locus_tag="ACHAUN_002508"
     CDS             86..787
                     /locus_tag="ACHAUN_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8461170.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1396..2412)
                     /gene="tsaD"
                     /locus_tag="ACHAUN_002509"
     CDS             complement(1396..2412)
                     /gene="tsaD"
                     /locus_tag="ACHAUN_002509"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8461171.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2409..2873)
                     /gene="rimI"
                     /locus_tag="ACHAUN_002510"
     CDS             complement(2409..2873)
                     /gene="rimI"
                     /locus_tag="ACHAUN_002510"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8461172.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2880..3422)
                     /gene="rimI"
                     /locus_tag="ACHAUN_002511"
     CDS             complement(2880..3422)
                     /gene="rimI"
                     /locus_tag="ACHAUN_002511"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8461173.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3406..4128)
                     /gene="tsaB"
                     /locus_tag="ACHAUN_002512"
     CDS             complement(3406..4128)
                     /gene="tsaB"
                     /locus_tag="ACHAUN_002512"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8461174.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4319..5299)
                     /locus_tag="ACHAUN_002513"
     CDS             complement(4319..5299)
                     /locus_tag="ACHAUN_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8461175.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5323..6807)
                     /gene="galT"
                     /locus_tag="ACHAUN_002514"
     CDS             complement(5323..6807)
                     /gene="galT"
                     /locus_tag="ACHAUN_002514"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8461176.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6819..7808)
                     /gene="galE"
                     /locus_tag="ACHAUN_002515"
     CDS             complement(6819..7808)
                     /gene="galE"
                     /locus_tag="ACHAUN_002515"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8461177.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2196 a         1695 c         1394 g         2571 t
ORIGIN      
        1 ttctaatgtt ggtatgttaa aactttacaa attctttaaa aaaagtgaca ataaatatag
       61 tgaacaataa ggagaggact ttttaatgcg tgttaataaa atcgattttc acttttcaaa
      121 actcagatat caacctgatt ttgaacattt ctcggatgaa gagttccaac agatcaagga
      181 acatactgtt cttcgttctt ataaaaaagg acagatcctt ttcgacgatg gtgatcccag
      241 acaacggttg tatatcttga ccaaaggact cgttcgttta gaacgttatg atgaaagtgc
      301 aacttattac tactatgact atgtgaatac aaataacctt tttcctatgt caggaatgtt
      361 tggagaagag acctacactt atacagctca agcgatgact gatattgaaa cattctacct
      421 tcctgtttcc tactatgaaa aattagcacg aaaaaatccc gaacagttga tctatatgat
      481 acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt atccaaatcg gattgacctc
      541 tagcgccttt gaccgggtaa aacaaaattt attcattttg aaagaagaat tgggcgtacc
      601 aacaaaagat ggtcatatca ccattcctta tccgatcacc ttaaaagaat tagcagtgaa
      661 tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa caactacgtg attccgggct
      721 gttagattat gacaaaaagc gttttacttt ctgtaagaca gctactactc cagctatgag
      781 agaataacgc gaacaaaaat aagcagtgac cctgataaat atcagggtca ctgcttattt
      841 tcctccctaa aacttctgaa agatccggca gttttagatc actggacaag actcaaagat
      901 tttctatgtg tgtttcagtt atgtaatatt cactgtttgc aaaaaatact gttagattaa
      961 ctagatgaag cgtcttgtcc tcatccgatc tagattattc gatattcctc gacaataaac
     1021 gattactcag tgagggcaat cagaggaacg gataatgata aataagctgt aaaaaatagc
     1081 aaaataacaa taatgattaa aagtatgtaa aaaatagggc tttgacaaaa acgtgtcaca
     1141 gccctatttc gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc
     1201 ataaacatga ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc
     1261 ttacgaactc ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca
     1321 ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcagc
     1381 tttttcacaa cctctttatt cagataaaac caagcttgga tcagcattca actgatagct
     1441 agcaaaattt cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc
     1501 tccacaaagt cgaagagggg gaatcactag ttctacctca gggagtttag ttgccaaagc
     1561 atcctgcaat ccttcacgca acccttgatt ggccgctaca cctccagcta taatcagttg
     1621 cttgactgga taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact
     1681 tgcttgaaag ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt
     1741 atgcaccaaa ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc
     1801 atggatcatc gctcgcggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat
     1861 ttcttttccg cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc
     1921 tgctgcgtca tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac
     1981 taattctgta tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac
     2041 cagacgggct gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg
     2101 tgcccaagca aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg
     2161 tccataagtg acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc
     2221 atcttccaag cataaggtga tttgttctac gtgatggcgg ctagctactt caggtactac
     2281 gccaccaaaa cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt
     2341 tccatttcta acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa
     2401 ttcttcattc atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc
     2461 gtcggattgc gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa
     2521 agttttcttg ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt
     2581 atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa
     2641 actgcaacag caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag
     2701 ccacagatct ggttatcttc agctaaaagc agataatcag agtgaggctg ttggatgtct
     2761 gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc
     2821 catacgcatt ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct
     2881 cactcatttt ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat
     2941 ctttttttga tcgttgttgc ttgaaacccc aatttccgat aaagtctttg tgcatcttga
     3001 ttactcatac gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg
     3061 atttcgtcaa taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata
     3121 ttcgtgatat ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca
     3181 ttatcaaaga tacctagata tagatgagga agcggtgact tcaattctga tataaaggca
     3241 ctttttgtcc atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg
     3301 atatcttctg tagtcaattc tcttagtacg taacgacgat cgatttgttc gactgttttt
     3361 tctgtataaa tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac
     3421 ataactttcg tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctactcgttt
     3481 taaatagcga ggaagaaaag cttgtttctc tgcaactggt tcaactaaag cacctaattg
     3541 agccacagtt acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg
     3601 caatatttcc tttatttcag agaagaattt gtgacaatca ctcccgacga aataaatagc
     3661 ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag
     3721 tacagtttct aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg
     3781 agcatcaaac atgggcacaa taatattatc cacaccgatg caattagccg cgatggtttt
     3841 cagactagat actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt
     3901 tacacctata cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat
     3961 gtcttttgga cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac
     4021 actgtggttg cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac
     4081 accgatcgcc aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct
     4141 ttctgctatg acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat
     4201 accaaaggca aaactagtac aataatacat gattatctgc tggcgggcaa attttgcccc
     4261 ttcctgaaac acaaaaaagc tgcggtatca ctccattttc ccacagcttt tccatctttt
     4321 atttcgttga ttctcgaata atcaactctg tttccacaat aatctttctt gaaaccggtg
     4381 ctttggaatt acttaattga agaatcgtct ccactgctag ctctcccatc cattcggtat
     4441 aaacttttac agtagaaagc gagggcgtta catatttcgc tacactgaca tcattgaagc
     4501 ccatcacaga aatatcttct ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc
     4561 caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta
     4621 aatactgctt cactgcttga tatcctgact cgacagaaaa atctgcttcg ataattggac
     4681 cttcgtcgta gcctttttct gccaaaactt gttgaaatgc cataattctg ggatctttta
     4741 gccgctgatg gtttttcttc gtatattctg ttccagcaag catagcgata tgttgcgctt
     4801 tatgggagac aaagtgttca atgacttttt gaacacttga cttaaagtcg acaactagag
     4861 agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt tgggatcctt
     4921 tcatttctaa taactgagct tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa
     4981 gatcactaat ggaatcccac gtttctttca ataaagtaac gtttgcttct tctgcctttt
     5041 tttcgattcc taggcgaata gataaataat aaagatcttc aagctcttcc gtatcgtcgt
     5101 accactggat caggcgaagc gtttgcttag tatttgctgc atttttacga tgtttcgtat
     5161 aatttaagcg ttctgctgct tcaaaaattt tttgctttgt ggctaagcca acggaaagat
     5221 caggatcata gttgagaact cttgaaacag tagcaggaga aactcctgcc aactgcgcaa
     5281 tgtctttaat tgttgccatt tgtctctcct ccttttttac gtttataaaa tcgcagtaaa
     5341 tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat actccagcgt cttccagtac
     5401 acgtgcaaaa acctgaccta cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa
     5461 atcaggatgt tcttttttta attgttctgc ccacggacga tgatattcag ccatctggtt
     5521 ttcttctcct aataagaaat gctctacttc tactagttct ggtttcaaac gaggtggtaa
     5581 aacagcaagc cccattactt cgatcaaccc aatgttctcc tttttgatat gttggacatc
     5641 tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa acattgttgt ctcgaaggac
     5701 aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg
     5761 tgtaccatct gctgaaaaag ctctgataga tactgtttca tccgaatatg ctttccattt
     5821 ttccaatact gagttagctg cttcaataag caagtttttc tctgttgatt gcaaacggat
     5881 cacagacatc ggccatttca cgactcctgc gatgacttgc ggataatctt ctagtttgat
     5941 aagtttttct atcggtgctt ttgccatagg aaattcatga cgtcctgcct gataatgatc
     6001 atgagaaaga atcgatcctc caacaattgg taagtcagca ttcgaaccaa caaagtaatg
     6061 aggcaagact tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg
     6121 gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa taagcataag gggaatattg
     6181 gaatccccag ctttcaccgt ctagattcat tcgaataatc cggtgattgg tgcgggctgg
     6241 atagttcaag cgacctctat atccttcgtt ttccatgcaa agcatgcagg taggatagtc
     6301 tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg gcttagataa
     6361 attgattgtg atttccagtt caccatactc cgactctgcc ggaaatttga tgttttttgc
     6421 gatggctctt gttttgatgt agtcattttt cttacttaaa ttaaagaaat actcagttgc
     6481 ttcctctggg cttttcgaat agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg
     6541 tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt tctgctggca attctgaaag
     6601 aacaccattt tctcttgcct tagcgacaag ctgatccaat aagtctacag attcttcctt
     6661 aactggtctt acttcaatgc tttctagagc atcttctcct accatcccta aaagacgatt
     6721 ctgtaaatat aagcggtcca tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa
     6781 atcacaaatc gtttgactga ttgacatatc tcattcccct attcttcata accatttgga
     6841 tggctgacat gccaatccca tgctgttttg ataatcgctt tgatatctgt gtattcaggt
     6901 ttccagccca gaatctctct cgctttttca cttgaagcga ctaatcggct aggatctcca
     6961 gctcttcttg gtgctatttc tgctggaatt tcttttcctg tcacttctct agctgcttct
     7021 agcatttcct tgactgagta tcctttgctg ctgcctaaat tgaagaagtt gctttcattt
     7081 ccttctttca agtattccaa agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca
     7141 taatctctga tacatgttcc atctggtgta tcataatcgt ctccataaac agccaaggct
     7201 tttctttgac caagagctac ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga
     7261 tcttctccga tagatgcatc tgcttttgct ccggctacat tgaagtagcg caaagcgaca
     7321 tagcgcatgc catatgcttg gtcacaccac ttcatcattt tttccatcat caatttactt
     7381 tcaccatacg gatttttagg attagctggt gtatcttctg tgattggaga ctctttcggc
     7441 tctccataag ttgctgcagt agaagagaat acgattttat tcacgttgaa ctcgtgcatg
     7501 acttcaagta agatttgcat accgtatacg ttgttgttga aatacatcaa tggtttttcg
     7561 acagattctc ctactaaaga gcttgcagca aaatggatta ctccttcaat cggttccttt
     7621 tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc cttcataaaa atgagcatct
     7681 ggatgtactg cttgtttgtg acctgtgagc aaattatcta ccacagctac ttgatacccc
     7741 ttttgaacta gctggtctac agcgtgcgaa ccgatataac cggctccgcc tagtactaaa
     7801 attgtcataa aaaacactcc tttgattagt aaaattgtgt ttgttgattc tatata
//
LOCUS       DAYYBG010000114         7631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000114 DAYYBG010000000
VERSION     DAYYBG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.117"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..2346
                     /locus_tag="ACHAUN_002516"
     CDS             19..2346
                     /locus_tag="ACHAUN_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM8461178.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2343..3257
                     /locus_tag="ACHAUN_002517"
                     /pseudo
     CDS             2343..3257
                     /locus_tag="ACHAUN_002517"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3330..3845
                     /locus_tag="ACHAUN_002518"
     CDS             3330..3845
                     /locus_tag="ACHAUN_002518"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8461179.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3842..4288
                     /locus_tag="ACHAUN_002519"
     CDS             3842..4288
                     /locus_tag="ACHAUN_002519"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8461180.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4438..5253
                     /locus_tag="ACHAUN_002520"
     CDS             4438..5253
                     /locus_tag="ACHAUN_002520"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8461181.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5459..6631
                     /locus_tag="ACHAUN_002521"
     CDS             5459..6631
                     /locus_tag="ACHAUN_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8461182.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6793..7287)
                     /locus_tag="ACHAUN_002522"
     CDS             complement(6793..7287)
                     /locus_tag="ACHAUN_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461183.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7284..7478)
                     /locus_tag="ACHAUN_002523"
     CDS             complement(7284..7478)
                     /locus_tag="ACHAUN_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461184.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2570 a         1443 c         1520 g         2098 t
ORIGIN      
        1 tttttattga gaggagggat gaaaatgatt aagaaaactc aaaataaaat ccaacgtata
       61 tgtcaaaaac attttgtttc tagactcaaa gctgtatcta aaagaaaata cttacatccc
      121 attatgattt cgatcatctt gatttttact ttcttaggga gcttatcaag tatttatgta
      181 aacgtttata cagaaacgtc aaattttgct aaccaaacga cagaggagac aaatagtcca
      241 ccctattctg aaatacagtc catagaaagc accaatcagt ctagcaacaa cgagacaacc
      301 tcaaccacat cctcgactca actgattgat ccgacagaaa aagaaatcac agcagcaaaa
      361 gcagcagggg agaaagctta ttcagaaaca ggtagaagcc agacatttag tactgttgcg
      421 gccactgcga atttcaacgg acataccata ccagatggat atacagtgat atatgccaaa
      481 gatggagtca ttgttgcggt taacggatcg atgagtgcgg ataaaactgc gttacaaggc
      541 gtaaccaaag caatcgcttt tattgataac cgtccagata tccataatct gtttattggc
      601 tccacagtaa atggtactac tatcacattc ccaaaaagta tctatatggg ggtggctacc
      661 gctggaaata cgatcgattc agttacaccc aatgcggata aaagttattc ttttacaccc
      721 tcaacatggg gagggcttcc tgccggagga ggtatgagta ctttttcaat cggtatacag
      781 ttagcaaatg atattccatt cacggggtac tcgatcaaaa atgggcagat cgttgatggt
      841 aagggaaatg tttcaaattt tccaacctat caagcagcaa cggttcaggt caatgcgaat
      901 cggacaggaa ctttttctta tacagtcaat ggtggagcag caaaaatcgg aacgtacggg
      961 actacattat ctggaatcca tgtgggtgac aaagtggtta tcactccaac agcagtaacg
     1021 ggctattctt ttgcacaaac aaacggttct ttcacagccc tagaaagtaa cgtcgatacg
     1081 gtgacctata caggcaaaca aacaccaatc actattaagt atcaagacta ttttggacag
     1141 atgattgctc cttctaaaat aatgaatctt acttacggaa gtgctgcaca agacctgacg
     1201 actaatacgc ctactatcaa cggatatacc tttacagctg tttcagcgat agaaacaaag
     1261 aaccaatcag caatgagtgt tttagctagt ttagatacaa atggaaatgt aatcgtaaag
     1321 gacgcaaatg gaaaacagat ttctgaagtc atcctatatt ataagacaaa tgcaacagta
     1381 tcaatcaatg caaatggcag taagtactat gatggtcttt ctgttgtacc gatcgttaaa
     1441 tatgccttta atgataaaac agaatcaaca tcccttttaa acaatgatct cagtattgat
     1501 caaatcacat ggaatccaac tgatttcaaa gcgatgaatg aaaagggagt tgaagtaact
     1561 gcgcctactg aaattggaac ctatacgtct tggcaattga caaaaagcgg actagctaaa
     1621 ctagctgcca gaacaaacta tttatttact atcgtgcaga catctgatgt ctatacgatt
     1681 aagcaaatca gcggtgcggt agagctcagt ggcagtaaga catatgatgg aaaagcaggt
     1741 gtcccgtcaa tccatgtcaa gctagcagag ggtgtcacta gttcgcttac tccagtacaa
     1801 gtggcactta gcagtatgga ttacacagca gatgcacaat cagcaaaaaa tatggttaat
     1861 gctggcagct atacaattaa actaacgaat agcggaatag acaaagtcaa agcagcaaat
     1921 cccaagttgt catttactga tatagccaac acatcaggaa cttataccat ctataaagct
     1981 aacgcagtga ttactgtcga tgacgctgcc tttaattatg atgcccaaag tcactcgatt
     2041 tcggttggaa atctccacgt taccggtgtt gtcttaggcg aatcattgga ttatacccta
     2101 acagttaaca gccggacaga tattggatca caaatagtaa cgattagttt aggcgaagga
     2161 agtgtaaaca ataattatac gattatcgca acagataccg ctaagttgac gatcaatccg
     2221 actctcttgc ttccttcgac aggtgggata ggtctactgc cctttgtctt tttaggactg
     2281 atctttattt tttctggctt cttttatttt atccatcgta agaaaaaagc aggtgaacag
     2341 cgatgaaaaa aacaaaaaat gtacgtgtgg atatatttgc tgttctcttt tttatcctac
     2401 tggtcttttt tataggttct ccgactgtgt cagctagaga atcacctatt acatttactt
     2461 tgcataatcg aatgttcgaa agcgataaga tgccaagtat cagtcaaaac gacggaataa
     2521 atatcccgga taactttcaa ggagtaaaag ggttgaacgg cgttactttc gctatatatg
     2581 atgtctcgga tgaattttac aagctgcggt cagaaggaag tagtgtggaa gacgcacaaa
     2641 gaaaactagc acaaaaaagt gatagtgaaa aaatactggc agaaaatgtg acaaaaacag
     2701 tagacgagga agagggcatt gcttctttta gtgcatcgga caaagatgag caaggtcgtg
     2761 acgctgttta ccgatttacg gaaataaaga cttcagatta agtaaaagaa aaatcagcac
     2821 catttgtcgt tgtgcttcct gtgacagatt catctaatca taaattgacg accatccact
     2881 tgtatccaaa gagcgaacaa aaaatacctg ctgcactcac attaacaaaa acagtagaga
     2941 ataagcagac agattttgca gatggggata aagtccctta tctcatcacc gtaacaattc
     3001 ctgagaacat caacgagata ggaacctata cgatcaaaga tacagctgat cctcagttat
     3061 ggctagaacc agaaacgatc gattttttga ttggtggaga caagattcat acatttcaca
     3121 cacaaaaaat aaaacatggt tttgtcttat cagattctgg aaaagatctt agttcgtttg
     3181 ctggaaaaaa attaacgatc cgttaccaaa tgacattgaa agaaaacagt gaaaaaacga
     3241 catttgataa cgatgtacgt ttgacgactg acaatcaaac agtagaaaca catcttgcca
     3301 tccaaacagg aggcaaacaa tttgtcaaag tggatcggca aacgaaacaa aaactagcag
     3361 atgctcaatt tctagtcaaa aaaaatgacc aatacttagc gcaagaaaac gggataaatc
     3421 attgggtagc caaagatgat tcaagagcaa cgatttttac ttccagtaaa gatggaacat
     3481 tttctgtcca tggattgtct tttggaagtt acgaactagt ggagattaaa ccgcctaaag
     3541 gatatatatt gagtcaatca accattctct ttgaagttga aaaaggcagt tatgatccgc
     3601 ttcatcctat accattggaa atcatcaacg aaccaaaagc aaatccgacg aataatggaa
     3661 aaacgccagt tcagatgcag acaaatggtc catcaaatga aaaaaatgga ggaagctttc
     3721 caaaaacaag cgaagagatg cttggaggtt tctcaatttt agggttattg ctagtccttt
     3781 caacagggac agcttggttt tataaaaaac aacaaaaagg aaacaatagg agggaaataa
     3841 aatgaaaaac atcaaaacaa aaatatggac gtttttagga actgctatca tgctgcttcc
     3901 tttcgttctc ggcctaggaa ctgcagaggt ttctgcagcc gtatcaccta caccagaaaa
     3961 tgttacggtt aatttgcata aattgaaatt cacttcagca ccagaaaatc aaataaacaa
     4021 cggaacggaa ttgacatttc cgaatagcga accgttgaac ggcgttgaat tcaacgtata
     4081 tgacatcact gctacgtact atccaagtaa agacacagca gttccagcag acgctacacc
     4141 cttcgcaagt gtaacgactt ctggtgaagg attagctaac ctgactcttc caggtaaatc
     4201 tgatgggaaa gatgctgtat acgtctttgt agaaacacca aagcccggtg tcgaaacgtc
     4261 gccaaatatc gtgttaagct taccctagac cctgacaaac ctttagacac gattcaccta
     4321 tatgctaaaa atgtcatcaa agaaagcaat atcaaaatcc agaaagctgg gaacttttca
     4381 ggggtagata agttagcagg ggcgcaattc cagatcaaaa attcagagaa tcaattcgtg
     4441 accggttttg atgaaaacac tggtgtcgct ctctatgatc ctgctgagtc agcgttgaca
     4501 ttcacaactg atactgaagg atccatcagt gtcaatggat taatagacgg caattatact
     4561 ttagttgaaa caaaagcacc agatggttat caattaacca atccacaaaa agaaactact
     4621 tttatagtaa aaaacggaaa attgcaacaa acaaccggta tcatccatgg cgcagatgaa
     4681 gccaatcttg ggaatccaaa cggtgtagtc aatcgggaaa atacaactac ggataacacg
     4741 attactgtag aaaatgtcca aaactacggt gatttccact tcatcaaaca agatgtgaac
     4801 acgcaagaaa aattagccga tgcagaattt tctattctaa gagcaaaaga tccaactgtt
     4861 aaattaatga aaaaaatagc aaaccttgcc tcaggagaat acccatatgc ttggtcagat
     4921 gaagtcacag gttctgactg ggaagaagtg aaattgattt ctgattcaga agggaaattt
     4981 tcaatcgaag ggctgaaaca aggggcatac tgccttcaag aaacaaaagc accagaaggc
     5041 tacgcattac ctaagaatga agctgcgtac actgaattta cagtagatga tgatccaaca
     5101 acttctgatg aaacaacgat cgataaccaa cctaaaggaa tcttgccttc aactggtggt
     5161 atggggatca tcgtattcat cttggtaggt actgctttag ttggaggagc agtgatctat
     5221 ttcaaaaaac gccatcaaga aacagaagct taaactattg gaaacaaaaa tcatacgata
     5281 aataaacgat agtgattcaa atggggagaa aggttgtgac aagtggtcgg tctcttggta
     5341 ttcgctcaac aaacggcaaa ataggaggaa ctattttgcc gtttgcttcg ctaatactca
     5401 ggtcaaggcg ctgatttcac aacctcttca tatacatatt agaaaatagg tggacatgat
     5461 gaataaaagc agaataagaa tactggacat cttcatggca atcattttag tcgttgggat
     5521 cgggatcttt tcttatcctt ttgtagaaga ttcactgaat gattttcttg cccagcaaat
     5581 gatcatccat tatcaaaagc aagcatcgaa taagaattca gaggaaataa aaaaacagca
     5641 ggaaaaaatg acaaagaaaa accaacagtt ggcagagcaa aacgtatcac cagggatagg
     5701 cagtttcaac caagcagttg atgcaaaagc attaaaagat ttacctagta atgccttttt
     5761 tatggaacat atgcttggag tcatcgaaat cccaaaaatc aatgtcagct tacctatatt
     5821 cgatcagaca acggaaatat ttttgcaaaa aggaacgtca ttattggaag aaagttctta
     5881 tccgactggc gggaaaaaca cccatgccgt tctttctggg catcgtggac tcccagaagc
     5941 aaaattattt acagatttgc ctaagttgaa aaaaggagac cagtttttca tccagatcaa
     6001 tggaaagaca ttggcgtatc aagtagaaaa aatccaagtg gtcttacctg atgaagtaga
     6061 ttctttgggt atccaaaaag gcagagatct cgtgactttg cttacctgta ctccttatat
     6121 ggtcaataca catcgactgc ttgtgacggg gcatcgtatt ccttatcaag caaaagaggc
     6181 aaaaaaagcg atccaaggaa ttgatcaatg gaaaaaatgg aaatttttta tttggtttat
     6241 cggtatacta cttggatcaa tcggcttagt ttggctactc atcgcttatt tagattcttt
     6301 ggccatcgct aagcgaaact atcctttatc tttttatgta aagaacacaa acggacgacc
     6361 aatcgaagga atggtctttt ctgtaaaaac gctcaacggc aaacactata ttactagaga
     6421 aaaagttcca tttgtgaaag cttctgatga atacggacta gtgagatttt ctgatttaaa
     6481 aggcaggaac tatcgcttgc aacatgagga gctcctgctt aaaatccatg taaaacataa
     6541 acattcgaaa cagttttcaa tgaaattgaa aaaaggaaga tataaactgc gaaaagaaaa
     6601 agaggtgtac tatttgattg agaaagaatg aatgaagatg tccaaaaaag cagagggaac
     6661 agactaaatg aaaaaagaga gaagctgacg aaatggacgt gtaaactgtt atttttggtt
     6721 tactatctgg aacgccatct tctatttttt tatgtttcag aagaatttta ttattatagt
     6781 ataacttttg aattagtttc cataacattt ggagcaaggg gttaaaccac gagcttgagc
     6841 ttcgcttaaa gtggcagggg tatacgtacc atttccacat ttatgtgtat ggtattttga
     6901 accggtcggt gtgacataga cagtttgttc attattttgc tcaggagctg cggcttgtgc
     6961 ctgctgagag gcctgttcag ctgctgattg ctgtcgcgcc tgttctgctt gttgctgtgc
     7021 gattgcttct tgcctcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7081 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7141 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7201 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7261 tgacaaagca gattccgctt ctgtcacttt ttttctagtg cagctttttc cgctttttgt
     7321 ttctcttctt ttttcttttc aatttgttct tgttctttcg atgaatctgc cgtactttcg
     7381 gagatcgtgg tactgctgct ggaagcctga tctgaacttg tgctttgagt agtagacgta
     7441 gtagcaccac acccactcaa caaacttcct gcgaccaaaa acaatgcaac taattttttc
     7501 gctaaatttc ctccttaagt aaaaaacaac cgttttcaat aaatgtcctt ttcaattgtt
     7561 tacttacttg ttataataat tgtgttatga atagtgattg aggaaactta atctaatccc
     7621 gtgttgatac a
//
LOCUS       DAYYBG010000115         7537 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000115 DAYYBG010000000
VERSION     DAYYBG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.118"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACHAUN_002524"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACHAUN_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8461185.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACHAUN_002525"
     CDS             1483..1935
                     /locus_tag="ACHAUN_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461186.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACHAUN_002526"
     CDS             1949..2638
                     /locus_tag="ACHAUN_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461187.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACHAUN_002527"
     CDS             2763..5114
                     /locus_tag="ACHAUN_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8461188.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACHAUN_002528"
     CDS             5194..5589
                     /locus_tag="ACHAUN_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461189.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACHAUN_002529"
     CDS             5914..6531
                     /locus_tag="ACHAUN_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8461190.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACHAUN_002530"
     CDS             6572..6871
                     /locus_tag="ACHAUN_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFM8461191.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACHAUN_002531"
     CDS             6905..7072
                     /locus_tag="ACHAUN_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8461192.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACHAUN_002532"
     CDS             complement(7117..>7537)
                     /locus_tag="ACHAUN_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8461193.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAYYBG010000116         7241 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000116 DAYYBG010000000
VERSION     DAYYBG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.119"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..508)
                     /locus_tag="ACHAUN_002533"
     CDS             complement(<1..508)
                     /locus_tag="ACHAUN_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8461194.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQ"
     gene            843..1277
                     /locus_tag="ACHAUN_002534"
     CDS             843..1277
                     /locus_tag="ACHAUN_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8461195.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1300..1620
                     /locus_tag="ACHAUN_002535"
     CDS             1300..1620
                     /locus_tag="ACHAUN_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461196.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1761..1952
                     /locus_tag="ACHAUN_002536"
     CDS             1761..1952
                     /locus_tag="ACHAUN_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461197.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(2023..2280)
                     /locus_tag="ACHAUN_002537"
     CDS             complement(2023..2280)
                     /locus_tag="ACHAUN_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8461198.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2438..2701
                     /locus_tag="ACHAUN_002538"
     CDS             2438..2701
                     /locus_tag="ACHAUN_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461199.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2725..2913
                     /locus_tag="ACHAUN_002539"
     CDS             2725..2913
                     /locus_tag="ACHAUN_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461200.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2996..3181)
                     /locus_tag="ACHAUN_002540"
     CDS             complement(2996..3181)
                     /locus_tag="ACHAUN_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461201.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3396..3767
                     /locus_tag="ACHAUN_002541"
     CDS             3396..3767
                     /locus_tag="ACHAUN_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8461202.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3917..3989)
                     /locus_tag="ACHAUN_002542"
     tRNA            complement(3917..3989)
                     /locus_tag="ACHAUN_002542"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3954..3956),aa:Gln,seq:ctg)
     gene            complement(4035..4463)
                     /locus_tag="ACHAUN_002543"
     CDS             complement(4035..4463)
                     /locus_tag="ACHAUN_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461203.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4523..5281)
                     /locus_tag="ACHAUN_002544"
     CDS             complement(4523..5281)
                     /locus_tag="ACHAUN_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8461204.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5576..5773
                     /locus_tag="ACHAUN_002545"
     CDS             5576..5773
                     /locus_tag="ACHAUN_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461205.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5879..6361
                     /locus_tag="ACHAUN_002546"
     CDS             5879..6361
                     /locus_tag="ACHAUN_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461206.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6419..7015)
                     /locus_tag="ACHAUN_002547"
     CDS             complement(6419..7015)
                     /locus_tag="ACHAUN_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8461207.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2406 a         1123 c         1446 g         2266 t
ORIGIN      
        1 attggtaacg gccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt gattcttttt
       61 gtgcgatcca ttcttttgca gagcttgtac ttgctggagt agcagcagtt tctgttacag
      121 caggagcagg ttgtgtttca gcaacaggcg ctgcttgtgt ttccgtttga accgtttctt
      181 gtacaacagg ttgttcagct tgaacaggtt cagcttggac tggtgcttct tgaacggctg
      241 cttgaggttc agcagctgct tgtgcagagc cttctgttgg gattgttaat tgttgtcctg
      301 agtagatcag gttaatatca gaaattgaat ttgattctgc aatggcattg atcaaggagt
      361 tatcaccaac gtatttttga gaaatcgttg atagagtatc acctgattga acggtataag
      421 cttcgtccgc atgagcagtc gttcccatga agaatgcagc gccggcagcc aaagttgttc
      481 caaaaagtaa agttttaagt gatgtcatga aaaatctcct ttattcgtta aaaagtttta
      541 tttgttgtgt tcaacgagga atactttaac acgtgaaaat atttcatagg taaaagaagc
      601 atgacaattt atgacaaatc cacgtttttt gtaacagaat gatgttcgct cgatgtgaaa
      661 aatgatgagg aatttcacat aatgcagaaa taatgggatt agaacaggtg ttctgatgag
      721 aaaagaaatg tataaataac ttgaaagaaa ttgcttttgt tcatcatttt atttacatat
      781 tgcgtatatt ttttgtattt ttgcttgaga tgttacaata aaagaaaaag gaggcggttc
      841 caatgagtaa aagataccag aacatcatgg tagcagttga cggatcaaga caatcgatca
      901 aagcgttcgc tgaagccctt gacttagcaa aggacaatga agcccactta tttatcgtgt
      961 caatcatcaa caaagttgag ctgacccata gtgcctatgc cttttcaaaa atctatgcag
     1021 atgaaaaaca aagaacagaa gtagccatgc tgaaaaaaat caatgatgcc aaagagtacg
     1081 gcattgctga gatccacgca atagttgaaa ctggagatcc aagaaatttg attgccaatg
     1141 tcatccctgg acaagaaaat attgatttga tcgttatggg agcgacagga aaaggtgcta
     1201 tacaacaagc attagttgga tcgaccgctt cttatgtcgt atcacatgcc ccttgcagtg
     1261 tgttggtcgt gaagtaatga gataagggag gaatagaaaa tggttcgtcc tcaagaagtc
     1321 cgtgcgccaa aagaaaaaat agaaatttta gcaattattg aagacggcac acaaacaaag
     1381 aagggatact cgatcgcttt agtaaagtgg aaaggaaaaa aaggtgttgc tctgagatgg
     1441 gatggagata accaacaaga taaaggattc cctgtcacag ctaacggtta tcatccagcg
     1501 tggtttgttc tgcctgataa atttatggaa ttatacagct atgattatgc aagagccgca
     1561 gcatcaatca aagcattaga taaattaaca gaagagcaga atgaaatgga ggaaaaataa
     1621 aaaagacgaa ccaatcagcc tttagaaaac tgattagttc gaggagtaaa aatgaaaaaa
     1681 ggttatttgg gatgaaacta gaataatgcg tataacttaa ataaaactta aagcaataaa
     1741 atggcaagag ggtgaggcaa atggaagcag gaaaagtatt acaggaagta aaagcactat
     1801 gtgcagataa aaaatatgaa gaagcgaaaa gcctgatcga aaagaacaaa gaaaatttgg
     1861 aagaaaagta cccgatcgca caagaattta tagaattaaa acaaggaacg atccttcacc
     1921 gattcaaaca tttttttggt ataacagaat agaaaaaacg attaaagatc ggcagaaatt
     1981 tgctgcatct ttaatcgttt tctttttaaa catgcgaggg attcaattct gattcttata
     2041 taaaaaatag atccaaataa taagaaaaat tactgaaagc gtcaattcga tgatgtgtgt
     2101 tattaataca gacgttgttt cccaatgcct aaaagaaaaa atttgataag taaaatatac
     2161 agcaggtaaa gaggccagta aaactgaaaa cttctttttt gtccatttta tgtaatagaa
     2221 aagaatgctt atcataataa aaaaaggaat ataaaaaaca aattgaccga tcgcgatcat
     2281 actattgcct ccgataaata aagtccatga tacaaaataa cactttgtgc gaaatggcgc
     2341 aagcagtttt gtggatttat ccggtaaaat ctgtatcgat tttgaaatta gaggagaaac
     2401 ttgctataat caaagtgaaa gaaggagtgg gattttcatg gatcatgtag tgttagaaat
     2461 cgatttgcat ttgatcaata atattcgggt gatttactat gttgtaagta acagtgtcga
     2521 acaacgtgta ctgacgaata aaataaatgg tattttagca aagaaagacg ttcaccgatt
     2581 caataatggt gaaggaagtt attatagtat cccagtagaa aaaattattt atacgacagt
     2641 caaagtcaga gaagatctag ataccaaaaa agcatatgaa cctattttta caacttattg
     2701 attgacagca gatggaggaa aaaaatgaat ccatatgaac aacttattca agatttgatc
     2761 gatggaaaaa tcgaaaagat tgatgtaaaa agagaagaat taatggcttt tcgagaagcg
     2821 tggctgaagt tggagaatcg taaatatgtg gtgggaaaag cctcactgaa cggcaatgtg
     2881 acttatcatt acgatccgac acgtttattt tagtatgaag tattcagaaa agaaccaaag
     2941 gaggacgatg cagcagcatt gtcctccttt gattttacaa aagtgaaagt tttacttatt
     3001 tgaatagatt tttaaatttg tttttgacac tgtcgatatt tacatctttt aaaagatttt
     3061 gggctttctc tttataaggt cctaattgtt ctttatgttc ttcgataaat cgttttgcgc
     3121 catcgaaatt gccgttctta atcatttcct tgactttttg aaataattct tcttgattca
     3181 ttttgaacac atcctttgct atcaacatga ttttttacat ggacaaaacg agaaaaactg
     3241 atttatccac acgtcaataa tatcaaataa cgatcatttt tactaaaaaa acactcgatt
     3301 ctcgcttcta aagtaggaga aaaatctcca acttgggacg gatggaattt ctttaactga
     3361 cccatatact gaatatataa caaggaggta gaaagatgaa agtgattaaa tatttgattg
     3421 gactggcggc gattagtggt attttgctat ttggactgaa atattatact gaaggaagtt
     3481 atggagaaat ccctggtata cttgatcagc taaatccatt agttgaaaaa ggagaggtct
     3541 atgtaaagac acaaaaacca gaggaagtaa atgaatatgg tacagcaagg tatgtgcaga
     3601 aagctgctga tgccaatggg aaagaacgaa cggttgaatt taatggtctt tctgtgttaa
     3661 aagaaaatca ttatttgaaa attagcaata aaggagcaca catagaaacg tatgaggaag
     3721 ttcctagaga aaaagttccg gagaaagcat tagcaattat tgggtaaatc agtttttgta
     3781 ttaagaatac gcccgatttt attattcata atttaattat gtaaactaaa tacaagtagt
     3841 aaaaataata caataatccc cctgcaaaaa gattgttctt ttacaggggg ttcatttttt
     3901 cagcaagcac atcaaattgg gatggcaggg ttcgaaccta caattgcttg attcagagtc
     3961 aagtgcctta ccagtttggc tacatcccat ttattcaaca aaataatcat agcactttcc
     4021 taaaaaaacg caagttattt ttcttgatcg gatagttgtt gctttactcg taaaaggcgg
     4081 cgtactagtt tcttttctaa tccagcagga agggcaatca gttctgctac tgacaagacg
     4141 gtgtccaata actgatattc tttgacaggt tcattcataa tatgatagat gtttgctaaa
     4201 cgttcaatat attcaaagcg agtatactct gttcgagcaa ttagaaattt caataaatcg
     4261 gtggctttct cgtactcttg atctgcgtaa taaacaagag attgacgata caatttgatt
     4321 tccagttcat tgagggtagc aggtctgcga cgctcgttcc actcattaat agagacttta
     4381 ccttgaacat ttcccttgca gtcacggaca aaaataatcg gaatttcatt tggatccatt
     4441 tttttatgtg tgaatttttc cattatctac gctcccttca aaggtttggt atcttgagta
     4501 taagggattt ttgtttgaat tactagcttt tctccaaagt tagcatctgt ccacccattg
     4561 cagtgaaaat cgccgaaaaa tcgttgatcg gtactgctcg attttttaca ccatagggat
     4621 cgttgacata aatattttct ttgtcatatc cagtgactac acaagcatga acaagaggag
     4681 tgactcttac ttctccatta tttgtggtcc aagtcataaa atcagtttca tctgggactt
     4741 caaaattgat agtcacagaa gtccagacag gatttccttt agatagtgct tttttgatat
     4801 catcgagttg agcatcggtt tttggaaaag tgtgataatc cgtttggacg atttgctgag
     4861 caagggaggc aactggggga acatagacga ccatagccca gtctccgcca ctgatatcac
     4921 caacaaatcc ttcattagga tcgccatgag tcccatctgc attgaaaaca gggacgtaat
     4981 ccaattgctc agctaactgg tttttagttg tttcataacc ataaaaagat aaaagcatag
     5041 aaagcgaagt gacttcacag ccattttcta aagaaggaga atcaaactgg ctctctaatg
     5101 gaacatctag taaaacttct gctttctctt ttgtcttttt cgttttttgt gttgaacttt
     5161 tttgtgcagt gttcattgta tgttctggag aatggatatg taggtaataa cttgtaccgc
     5221 ttaataaaag agcagctgtg ccccctagaa acaatagttt tttgaacgtt tttttcttca
     5281 tttgaaatct cccttttcag ctgtttatct ttcctattat aaatgtattt ttattggaaa
     5341 tggaagtatt atattttaat atgtaggaaa taattagttt ttaaggaata tgttttaatt
     5401 ttttgaaaaa aattcttcta aatacaacag ctgttttaat tgtgaaaacg aaaacagtac
     5461 gctataatgc ctgtaaggtt gatttttaac tgtaaataaa aatcaatgag ttaaatctcc
     5521 aaagaaatag cagtggtaat gtggagaaaa gttccagcag tttaggagga ttttgatgac
     5581 ggaagaaatg attaatttag gagaacaata tgcatgcaaa ccaattggtt ttacaaaaac
     5641 ggtcattggt gaagttgtat caaaaatgac aaactgcgcc gtggtcaaag ttgcccaatg
     5701 tgctgcagag gaccaagaat tattagatga aaaagcaagc atggttgttg caaaatatga
     5761 tacatttgaa taaagaaaac aattggacgt gcggcgtgct gcaagtccaa tttctttgag
     5821 aaggtaaatc agtattcttt tcctattttc tggagaagaa aaagcgaggc gagttgtcat
     5881 ggtaacattc tttacggttc tattgttttt attcgttgtt gtgatgtttt tatttctttg
     5941 gctggttcga aaagaaatta tttatcggac tgtgcgaaat cgatgggtct acttggtgat
     6001 acccttctta gtgtcactag tcatttggta tacactcatc agtcatccta cagcagatga
     6061 attggcaaaa gggatcttgt cagcaatgat ttttattagt tttttgttgg acagtagagg
     6121 aatcacagaa gaaggacttg ttttgaatag ctttgacaaa aaaggtgttc ctttttctga
     6181 gatcaataaa attgtattat atcagccaaa aggttctaaa atcgtaaaaa tgaatttttt
     6241 ccgaaacgga tggcgaggac cgatgctgaa attttctgca tcattagaag aattagttcc
     6301 atttttgtcc caacgcttaa acgatgaggc agagattgat attatgattg atccagaata
     6361 aaaacagagg ttgcgacaag atgtttgtca caacctctgt tttttatttc aaataaaatt
     6421 atccaaatac gcggaagcga tcggcatcgt tcatgtattc tttttcctca gccggtgttt
     6481 ctggtgaacc gaatactaat tgcgaacgta gtttccagtt tgaagggata ttccattctt
     6541 ttgcaactgc ttcgtcaata actgggttgt aatgttgcaa gtttgcgccc aatcctttat
     6601 caactaatgc tacccaagta ttagctgttg cgattccgtt tgattgttct gaccaatctg
     6661 ggaagttgtc tgcatataat tcaaattgtt cttgtagacc ttttaccacg tcagtatctt
     6721 tgaagaataa tactgtacca taaccatttt tgaaaccagc taatttgttt tgtgtatttg
     6781 ggaaagcttc tgctggagtc aatgggcgaa gtgcttcttc agtgatttcc caaagttttt
     6841 catgtgcatc tccgaaaagg ataactgcgc gtgttgattg agcattgaaa gcagttgggc
     6901 tttctttaat tgcttctttg atcaaagttg ttaattcttc attagaaagt gaaacattac
     6961 gtcctagatg atagattgaa cggcggtttt tcaaagtgtc agtaaaagtt gtcataataa
     7021 aatttctcct ttgatttgcg ttttgcattt ttttaattga ttacgaaatg aagtatagca
     7081 ccttacaaaa agtaagtaaa cagatatgct cacaaaaaat aagcgaagtt caaaaaacaa
     7141 ttttaataag aaaaatcgtt atgtaaggat tttcttagtt atcgctttca ttgttgtgtt
     7201 ttaggaattt ttgcttattt gaaaaaaaca gctgatatat a
//
LOCUS       DAYYBG010000117         7217 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000117 DAYYBG010000000
VERSION     DAYYBG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.120"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACHAUN_002548"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACHAUN_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8461208.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACHAUN_002549"
     CDS             complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACHAUN_002549"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8461209.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACHAUN_002550"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACHAUN_002550"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8461210.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACHAUN_002551"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACHAUN_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8461211.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACHAUN_002552"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACHAUN_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8461212.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="ACHAUN_002553"
     CDS             complement(4433..5290)
                     /locus_tag="ACHAUN_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8461213.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACHAUN_002554"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACHAUN_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8461214.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DAYYBG010000118         6915 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000118 DAYYBG010000000
VERSION     DAYYBG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.121"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..768)
                     /locus_tag="ACHAUN_002555"
     CDS             complement(16..768)
                     /locus_tag="ACHAUN_002555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8461215.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1132..3798)
                     /locus_tag="ACHAUN_002556"
     CDS             complement(1132..3798)
                     /locus_tag="ACHAUN_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8461216.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4139..5590
                     /gene="cls"
                     /locus_tag="ACHAUN_002557"
     CDS             4139..5590
                     /gene="cls"
                     /locus_tag="ACHAUN_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8461217.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(5919..6734)
                     /gene="thiD"
                     /locus_tag="ACHAUN_002558"
     CDS             complement(5919..6734)
                     /gene="thiD"
                     /locus_tag="ACHAUN_002558"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8461218.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         1969 a         1469 c         1196 g         2281 t
ORIGIN      
        1 cataaaaaga acccttcacg agtcgtgaaa ggttcttttt ttatctgcgg accaaaggag
       61 cagtctatag gtcagcttag tcgtactaag gatttctcgt ttttcactga atagtttgcg
      121 aatgtacctg gtcacaacag gatcttttgc gtacgcgtca tagtatagct tcatcaattc
      181 ttcagtaatt gattcctgtg taaaataagt aaaatctttt ttcttcactt tgaatccttc
      241 atcaattaag tatgtttcaa attgatccat tacaactggc gatgtattag gatcttcttc
      301 tatcattcct agttgctttt cgataggata aaaaacatca tcgattgatt ccactactct
      361 gattatcacg caccatttgc ttgacttttt catcaatttt tctaattgaa taagatgtac
      421 caatgctgga tttgctgctg taaaaaccat ttcatatgtc tcatttgatt ggataaattc
      481 ttctaacgac tgtttgataa aggaaacatt tggcaactca tttttttctg cttctttttt
      541 tgcattttct agcattcctg aagaaaaatc gatcaaatca tagcgattaa tttcttttgc
      601 taaggaaaga aatctgccag atcctccagc aacgtctgct gctgtttcag caggcaggac
      661 tttctccgtt ttcagataat tttttacatc ttcagcaata gttacttgcg attcttgctg
      721 gatttcataa tattcttcag caaattcatc ccattctttt tcattcattt gatttcctct
      781 aattttttgt tatatgtttt cacttttcgg aataaatcat tctcctcttt tatctattct
      841 atctcttttg atgaataata aaatgattgg ctttccgtct atcataaata aaaaaaggga
      901 ctgtgaccaa agtcgtgtca aagtccctct ttccgaataa acagtgtcca atcagatgac
      961 cctcagtatt agctcaaaat gctgatttta caacttcttc cttaaacggt gttctcctgc
     1021 caactcttcg gtcacaagtc acaatattca acaaacatga gaagctgttt gttgaaattg
     1081 caccttgtga ctcagaatta aacggcaggc tcacaacctc gttgattcgt tttatttatt
     1141 tccttgtact cgtacaatga tcaatttagt gatttccatc aaaacaattg tcacaagtgc
     1201 gattcccata catgtaaaga cttctgtcaa tccaaatgct gatgggatag agaagacttc
     1261 tttcagtcct gggatcattg ttaagcagta aagcgcgcta catacaacta cagctagtaa
     1321 gacacttctg ttagagaaga accctgcttg gatagctgtt tgtgtgtttg aacgtgctgg
     1381 gaatgtttgc aatgtccgac accaaatcaa tgttgaaaat gacatggcta caccgaattc
     1441 aggtgaatgc tgcatgccaa gccattgagc aaagataaca gcaatcccaa tcagcacacc
     1501 acgatacgtt acagaaatca atgttttgtc cgtaaagatt cctgaatttg gatctcttgg
     1561 cgggcggttc atcgtagcag gttcagcttt ttctgtacct aatgcaattg ctggcagtga
     1621 atcattgact aaattgatga acaacagttg taatgctgta aatggattgg accagtcagc
     1681 aactaaggca aagatgatcg caatgatcgc acctaaattt cctgcaaata gataagaaat
     1741 agctttcttg atattatcaa acacattacg tcctacttct actgcgctga caatagaagc
     1801 aaagttatca tccgtcaata ccatcgctgc tgcatctttt gccacatctg tacccgttcc
     1861 catagcaatt ccgatgtctg cttgtttcaa tgctggtgcg tcatttacac catcacctgt
     1921 catagctgag atatgacctt tattttgcca agcacgaacg atacgaattt tattctcagg
     1981 tgaaacacgt gcataaacag tgactttttc taaaacttcg tttagttgat cttctgtcaa
     2041 ttcatctagt tccgtcccgg tcaaagccag gtttccttct tcaaaaatcc cgatttgttt
     2101 tgcgatagca acagctgttg ttttgtggtc accagtaatc atgattgttt tgattcctgc
     2161 acttcttgct tctgttaccg cctgagtcac ttcagcacgt ggaggatcga tcatagcaag
     2221 caaaccaaca aggatcaaat cgtcttcatc ttcaaatgat aaatcaaaat tatcaagtgg
     2281 acggtaagca aaagctagta cacgtaatgc gcgtgttgaa aaagcttcat tttgctcttg
     2341 gaattttttc atcaattcat ccgtcatcgg aacgacttca ccatttagca atactttttt
     2401 actgcgttgg aagacgatat ctggtccgcc tttaacaaac aagtaatttt tgttatcaat
     2461 tgtgtgtcca gtagacatta attttcgatc tgagtcaaaa ggaagttctg cttgtcttgg
     2521 gtagttgttt cggatttcct gataaggctg gtttaatttt tcactaaagt cgataaacgc
     2581 tacttccgta gggtcgccca atttagtacc gtcttcactg atcgatgcat cattcgctaa
     2641 aacactgatt tcgatcaagc gacgttcgtc agctgtccat tttgaagggt catttgtgaa
     2701 atccccagta tcgccattac ctaaaaagta gtcaacaact gtcattttgt tttgtgtcaa
     2761 tgtaccagtc ttatctgtac agatgatact tgtcgaacca agtgtttcaa ctgctggaag
     2821 cttccgaata atggcatgac gttttgccat tttattcgtt cccaaagata acacgattgt
     2881 tacgatagac tgtaatgctt ctggaatagc agctacggct acggctacgg caaacatcac
     2941 tgcgctgacg atatctgcag tcatatctcc tgtaccttct ccaagataaa tacgaaacag
     3001 ttggatacct aaaatcaata aagacaatgc caaaatagcc aaacttagtt ttttactgaa
     3061 tttatccaga cctcgttgta acggagtgac ttgctcagtt gttgattcca aaaggccagc
     3121 aacttgtccg atctccgtat tattcccagt tgaagtcact aagaatttac cgcgtccgta
     3181 agttacgatc gttccactga ataccatatt tgtacgatct ccgattggta ccgtacttgt
     3241 gatatccgtt acttcttttt ctgcagaagt cgactctcct gtcagcatac cttcatctac
     3301 ttttaatgat cccgcatcta gtaaacgtcc atcagcaggt acataatcgc ctgcgtctaa
     3361 caacacgata tctcctacca caagttcgtt tgccggaatc gttttttcct taccatcacg
     3421 aatcactcga gcatcgggcg ctgaaaggtt tttcaacgct tctaaagaac cttctgcttt
     3481 ttttgtttgg acaacactga ttatagaatt caacatcaat actgcaaaaa taactaagaa
     3541 ttcgatgtag tcacccatga ccatctgaat cagtgcgaca atcagcaata cgataaccat
     3601 tgcatctttg aaagtttcta agaacaattt ccaagttggc ttcttcggct tttcttttag
     3661 ctggttcaag ccatctcttt ctaatttttc cttcgcttgc gtggtgctca atccatcggt
     3721 tgagctctgc gtttgctgca tcaacgagtc gatcgtttct ttatagaccg cttgttgtgc
     3781 ttgttccttg ttttccaaat ttcatccccc attttttgat cttggcccta gtcctgtctg
     3841 aaaaaaaaga gacctaagac aaatacgcat ttgtcttagg tctcaccatt tcatacaata
     3901 ccagaaaacc atttggttaa cttactgttg atattatcgc aacaaacgtt gccggttact
     3961 cccctaagga gatattcaac tgtactaata tcctacctta ttaacctaca aaaagcaact
     4021 actttagccc gatttaccta cattttcatg aaaggagtga aaagtatatt caccttggta
     4081 ggcttctttc ttctatcatg ctatactaat gttattgtat gtaaggagga acatttttgt
     4141 ggtatctagt attataaccg ccctttatct attaaatgca cttattgctt tggtggctat
     4201 tttgatcaaa ccccgagatg tagcagccat ttgggcatgg ctcttagtat tgtttgccct
     4261 tcctggggtg ggctttgtat tatatttatt tttcggacgt ggattaacgg ataagaaaaa
     4321 attctatctc cgacaaagtg acttgaaaga attagaaaac tttcagtctt ttagaggaga
     4381 taccattgaa cattacgatc ctgacatggg cgataaagac aaacagcaat ttgttgactt
     4441 cttctcttca ttaaatcgta tgccgctgac aagaatgaat tctgtcactc ttctcacaga
     4501 cggacaagag aaattggatt cactgcttca agatctaaaa aaagccaaac attcgatcca
     4561 tatcgaatat tacgcatttg tgacagataa tatcggccag caagtcttac atgttttaga
     4621 agaaaaagcc gcagaaggcg tggaagttcg aatattatat gatgcatttg gctctcatgg
     4681 cacaaaagca aaagatttca atcgtctaat caaaaatggt ggacatgtcc atacatttgt
     4741 tacctcacaa agggcattac ttcgtttccg attgaattac catgatcacc gaaaaatcgt
     4801 tgtgatcgat ggaaagatta gttataccgg tggtttcaat attgccaatc aatatgtaaa
     4861 tacaacaaaa aaattcggct attggcgcga tacgcatata cggattttcg gtgccgcttc
     4921 ttctttgctc cagcttcgct tcttaacaga ctggaacgtc tcggtacctg aagaaaaaaa
     4981 ggtcggctat catttgaatt atttctttaa aaaagcagat cgagatgaat ctaagcttgc
     5041 tgatacatcc atccagcttg tttcaagcgg accgaataac gaaagggaac aaatcaagct
     5101 ttcatttatc aaattgatta cttctgctaa aaaacgtgtt tggatacaga caccttacct
     5161 tgttcctgat gaaagtgtca ttgctgcttt aaaaatcgca actgcctctg gtgtagatgt
     5221 gaaaatcatg attcccaaca aaccggatca tccttttatt tatcgagcaa cacaatatta
     5281 tgctcggcag ctgatcaagg aaaatgtaca aatccttgtc tatgagaacg gcttcctcca
     5341 tgcaaaaaca ttgataatgg atgatgaaat ctgcatggta ggttcagcaa atcaagatat
     5401 tcgtagctac cgattgaatt ttgaaacaag tgctgtcatt tacgatcctg agtttttaga
     5461 agaacttgct actcagttca aagaagatga gacacattgt tcatccatga caactgaaac
     5521 agtcaaggaa atgtctaact ggctattatt caagcaacaa atttctcgat tattttctcc
     5581 aatcctataa tttttttatt caagtagaga aaaatatact aagattaaag ggactgtgac
     5641 aaaaatcttg tcacagtccc ttcttccgaa caaacggtgt tctcctgcca actcctcggt
     5701 cacaagtcac aatattcaac aaacatgaga agctgttttt tgaacttgtt ccttgtgact
     5761 cggagctgga cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
     5821 cggtaataag tccaaccaag ttaaaaacat aagaagctgt ttttacttgg ctgttcttat
     5881 tacttgatgc agggcaactt tttcacaact tctttctttt actctaattc accagctgcc
     5941 caatgattga tggggccata tttgtgccct acatttattt cttcacgaat tgcacggttt
     6001 acatagtctt ttgccaatcg gatcgcttgt tcaactgttt ttcctttcgc taactcagct
     6061 gcaatacatg cagatagcga atcgccagtt ccgttgatcc ggtcggtagg ataaaatggt
     6121 gcatttaacc aaaatgattt tcctgattcg aacaatacgt agtcattgac tgtttcttgc
     6181 tgcgcatttt tagcatgatg tcctttgatc ataacgtttt ttgcacccat atcttgcagt
     6241 tcttttgctg cttgcacaaa atcttcttca gctgctaatg tccgtccagt cagcttttct
     6301 gcttcataaa aattaggtgt cagtacttcg gccaaaggga caagcttttc tttcaagctt
     6361 tgcaaagcag attcttctag taaaagattt ccatgtttcg ttacgattac gggatccacg
     6421 actaatggtc caaaatcata tttttggtaa ttttttacaa ctgtctcaat caactttgaa
     6481 tctgctagca tacctgtttt agctgcacgt atttgaaagt ccttcgcaat cagttcaaat
     6541 tctttgtcga taaaatctgt tggcaatgtc acacttgctc cgattccgta agaattacct
     6601 gcaacacatg cggtcatgac agaaacaccg taaacatttc ttgtaaagaa tgtatgcata
     6661 tctgcttgca tccctgcgct tccatcgcta tctgatcctg cgatcgtcat tgcttgtaca
     6721 tactgatctt ccatcaaatc gattcctcca tttttataaa aagatttttc ataatactgt
     6781 tactgtttct atcatatgtt tttcaaagta tccctcagta aatataccac taccttttct
     6841 tggtaatcaa atttatttga aaaaacattt gcaataaatc agaaaatagt ggtcgtttat
     6901 ttgtattcgc tattc
//
LOCUS       DAYYBG010000119         6813 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000119 DAYYBG010000000
VERSION     DAYYBG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.122"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            527..721
                     /locus_tag="ACHAUN_002559"
     CDS             527..721
                     /locus_tag="ACHAUN_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461219.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            912..1161
                     /locus_tag="ACHAUN_002560"
                     /pseudo
     CDS             912..1161
                     /locus_tag="ACHAUN_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1405..2235)
                     /locus_tag="ACHAUN_002561"
     CDS             complement(1405..2235)
                     /locus_tag="ACHAUN_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8461220.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2443..3777
                     /locus_tag="ACHAUN_002562"
     CDS             2443..3777
                     /locus_tag="ACHAUN_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8461221.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3818..4729
                     /locus_tag="ACHAUN_002563"
     CDS             3818..4729
                     /locus_tag="ACHAUN_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8461222.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4791..5579
                     /locus_tag="ACHAUN_002564"
     CDS             4791..5579
                     /locus_tag="ACHAUN_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8461223.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            5576..6192
                     /locus_tag="ACHAUN_002565"
                     /pseudo
     CDS             5576..6192
                     /locus_tag="ACHAUN_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            6194..6712
                     /locus_tag="ACHAUN_002566"
     CDS             6194..6712
                     /locus_tag="ACHAUN_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8461224.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2045 a         1185 c         1367 g         2216 t
ORIGIN      
        1 ttattttata ataaagatat gatgactaat ttagctcaac tattccagcg tcagctgaag
       61 atattgcaat tcccgattcc ttaatcattt atctggatga gacagaaagt gaagttgcaa
      121 cagatgtgca tgtagttgag gataaaaatg atagtttaaa aattaatagt tcagatactt
      181 tactaagagt aagtcctatc ttttcagctg ttatttagat gcaagtagca actaagttag
      241 gtagtactcc tgcaatggat aggtatttgt gtgttcgata tataagtggt cgaagtatca
      301 aggttatgta tactggatgg gagatagaag agttaaaaat tggaggtcac cgccacaaat
      361 ggaatttaga tttagaggta ccttagattt ggtatagtta ttaaacacaa gatggcctgg
      421 tttgtaggga aagtcgaaga gtatttttcc acttctgcgc ccctttaatt tttctagtaa
      481 tgccgtgaat ttgtgattaa ttggaaatat gaagaaagga gagtctatga aaacaacact
      541 agttctattc tataaaaagc atccttattt tacacttctg ataaatatat tgttggctag
      601 tgttatcggg atttctgttg aatatctaat aaataaagat ttcataggtt cttgttttta
      661 tacggcttta tttcttggac ttcttgaagc attttccatt tataaaaaat caaaaaaata
      721 aaatagattg ccgtaagaga tctttttcat ttcatttttt tctgaaaacc cgttgtttct
      781 gtcagtgaag cagcgggttt ttagatttat tgtctttaga cctaattgag cgcgccgagc
      841 gattgaaata caaaaccact tgctatgcgg gtggagcaat aaagggaaat agtttagccc
      901 gtacaaagta gatgtgtaaa taccacattg tattcacacc aaagtataga agaaaaataa
      961 tttataatca atatcgagcc aatattcagg agatcatcaa gagattgtgc aaatctaaaa
     1021 tataaatttg ggtatcgaca cttttgggtg gaaggatact atgtgacaaa agtaggacaa
     1081 aatgaagcga ccatcaaaaa gtacattcaa gaacaagaaa aacatgatag tgcgttagat
     1141 aagctgagtg tacgagagta agacgatcct tttaagggct aaggagtagt acataaacct
     1201 tttaagaggt gggcaaaact ggcaaaggca atatggcttg aacataaaat atgaaagcca
     1261 gcgtctttag gcgctggccg gtgatttgag cttatagctc tagagaaaac cacccttttg
     1321 aagggtggtt atgattaatg actttagtca gttttgctca caaagcgtgt ggaacaaaaa
     1381 acgggtgttt ctacgggtag atatttattt cttgaatgga ggtgtcccat acatttttgg
     1441 tgttactggt tctaattttg gtgcagcgcc ttcgatctta ggttctttca accattgata
     1501 ttcgccttcg ccatccagtg ctttgccact agcccaagaa ccttcactac tttggtctcc
     1561 ctctgagaag ttatagaagt cataagcaat gtcttttcgt tctttgtcac gcgggaaagt
     1621 acttgggact aagattgaac cttctttttc ttctaattct ttcactgcac attcccatag
     1681 attttgatga tagctatctc ttgcaaggag aacagacagc atatctttta cgccacgatc
     1741 atcgatcatt tcgtaaatac gtgctacttg aagtcttcct tgtgattcgg catttaaatt
     1801 agctctaaag tctgctaaca agttgccact cgcaatgata taggcaccgc tccatggatt
     1861 gcccacgctg tcagcaggac gaggtcctaa gccagctacg atcgcgtgct gtggattcat
     1921 tccagagata atggcaccga ttgttggatc tttttgcatc gctgcttctt gatcttcaac
     1981 aggcgcaccc tctaataaac gagcaaccat tgtcgccaac atttccacgt ggccgatttc
     2041 ttctgttgca atgtccatca gcatatcttt atatttttct tctcctcggc agctccagcc
     2101 ttggaacagg tactgcatag ctacactcat ctcgccatat tggccgccga tcagctcctg
     2161 cagatatttc gcgattaatg gattcggacg atctggtttt gcttcaaact gcaattcttt
     2221 ctggtgtcta aacatcaaaa ttcctcctaa tctattttta atacattctc attctgctac
     2281 gaaagattaa aaaattcaaa gaaagcgctc gcgaattata taaagtattg gtatcattaa
     2341 taattttttt agaatcatac cttataaatt gttattttca gaacaaaatg atatcgttat
     2401 attaaaaccg gcatcggtta cattaaaatt tggaggtaaa atatgaaaaa attattgttt
     2461 acagggttgc tggcaagttc agcattgttt ttattaggag gatgcggatc aagtaatgca
     2521 agcgatgcca gttcaagtgc agcaaattct tctgaaaaaa caagtgatgg aagaaaaaat
     2581 attgatttct ggtccttttg gggatccggt gcacgaaaag aagctatcga agaaatcatt
     2641 gaggatttca atcaatcaca aaacgagatc catgtaaatt ataagtatca gccatggggt
     2701 gacatctgga ctaaatcttt atctgcaata acagctggaa atgcacccga tgttattgta
     2761 caggacataa attccgttgc ccaaagagca gaagcacagc aagcaactaa tctctccaaa
     2821 tatgtagaaa aagacacttc taaggatttt tatccgcagc tttgggatac agtagaatat
     2881 gaaggtgatc cttatgcgat cccattcaat acagatacac aagtcatttt ttataataaa
     2941 aagatattta aagaagctgg aatcagtgaa caagatctac cgacaacttg ggatgaatta
     3001 gaagaaacag cacgtaaact agataaaaaa gaaggaaaag aatttacacg catcggtttt
     3061 tatccattat ggaatttagg ggctgatgta tgggcgctaa acgcagataa cgggactagt
     3121 tggtttgaca aagatgaaaa agtcaagatt gatactaaaa ataaagtgga agccttggag
     3181 tggatccaag actggcaaga ttattatggg aaaaatacga tcaataaatt agaagccgaa
     3241 tttgggtctg gtgtatctga tccatttatt tccggattag tagccatgcg tgcccaaaat
     3301 atcaattatt actctagttt gatggaaaat gcaccggatg attttgattt tggcgtgatg
     3361 cagctcccag aaaaagaaga aggttcaggc cattggtcgt ggggtggcgg attcgtcttg
     3421 gaagtaccat acggagcaaa agatcctgag gcctcttatg aattcatcaa atatctttct
     3481 tctccagaag tacaagaaaa attcggcgaa aaaagttttg atatcatggc gagcaagaca
     3541 gccaatgaga atttagtgaa taatgacaac ctaacggata aaggaaaaat gatctatcaa
     3601 atggcggacg aaaactttaa aaatacggtg atcacaccag ttcctttaga tgctccggat
     3661 tacacgacat tagtgaatga acagattgac cagattcttt taggaacaaa atcaccaaaa
     3721 gaaggattgg cagatgcaca aaaagcagta gaaaacttag tcaagcaaaa tcaataatcc
     3781 atttttgact gtctaggtac agaggaggca agaaaatttg aagcgtttat ctatccgaaa
     3841 gaaaaaagaa gcaagatggg cctatttatt tatctcgcct tttattatcg ggttcttggt
     3901 atttatgcta gggcctatgc ttttttcagt tattggtagt ttcaccgatt ataatttgac
     3961 atccaagatg aatttcattg gtctagcaaa tttcaaaaga atgttcgttc acgatgattt
     4021 attttggaaa tcgttataca acaccgttta tttcgtcatc ttcaatgtac cgctgacgac
     4081 gattttcagt gtgtttttag ccacagtact caatcaaaaa atcaaaggaa tcagtgtatt
     4141 ccgtacagct ttctatttac cggcagtctt atcaggagta gcagtctaca tcttatggat
     4201 gcagctgctt tctccatctg caggattgat caacacattt ctgggctggt tccatatccc
     4261 tggaccagct tggctgtttg atccacaatg gacgaaacct gcgttgattt tgatgaaaat
     4321 atggagttct ggaggagcaa tgctacttta cttagctaca ttacaaaatg ttcctagatc
     4381 tttatatgaa tcagcagaaa tcgatggagc aggcgtttgg ggaaagttca aaagtattac
     4441 tttgccatta atcactccta ttatttttta cgatgtgatc acctctacga tcggttcttt
     4501 ccaaattttt caagaagctt atgtcatgac gaaaaatgga acaggcggac ctgcaaattc
     4561 tttgcttttt tataatctgc atatgtggaa caaggcgttc gtagcttttg atatgggtta
     4621 tgcgatggct atgtcaatga tcctgtttct gatcgtgttg gttctgacgt ttgtcaattt
     4681 gaagcttgca cctaagtggg tctattattc tggaggtgac ggcaaatgat ggattatttc
     4741 aaaaagacca gtgtgaatcg gcaaacaaaa aaatactaag ttatgtcctc atgacagtaa
     4801 taggaatcat tttgatcatt ccattgcttt ggatggtgtt tacttcattg aaaccaatgg
     4861 aagaaatcgt tcgttatcct ccgacctttt tcccagaaaa aatcgtatgg gaaaactatc
     4921 tggatacgat tgccgccttt cctttttggc gctatgcaag aaatacatta ttcatcactg
     4981 tgctagtagt gatcggtaat gtcctttcaa attcttttat tgcttatgga tttgcgaagc
     5041 ttgattttcc tgggaaaaag ctgatgtttg ctttggttct ttctacgatg atgatcccgg
     5101 ggtttgtaac gatgatccct caatatgtat tattctccaa gatcggctgg gtaggtactt
     5161 atttgcccct gatcgttcca tcgttcttcg gcaatgcgtt caatatattt ctgatgcgtc
     5221 aattttactt atcgatcaat aatgaattga tcgaagctgc tgaaatcgac ggagcgaatc
     5281 atttgtatat ttggagtcat ttgatgcttc cattgacgaa acctgccttg attaccattg
     5341 cgatcaattc cttcaacgca gcttggaatg atttcttagg tcctctgtta tatatccaag
     5401 atcaagaaaa atacacgtta cagattggct tgcaagtttt tcagaaccaa gcaacgacac
     5461 aatggaatta tctgatggca ggagcaacac ttgtcttgat cccgacgatt ttgctgttct
     5521 tttttgcaca acgttatttc attgaaggga tggatttgac aggaggttca aaaggatgat
     5581 gaatcgatcg attggtaaaa aagccaatga ttatttctta gaaatcattg aatggattcc
     5641 acgtcttatc catttacagc tactatggtt cctgtgcgtc ttgcctgttt tcacattagg
     5701 aactgcaagt cgaacagttc tgtatatgat ttatcattac aaaaaaatga agagaaaaag
     5761 ctcggttcac tattctggaa aaagtttcgt gagaacttct ctctttatgg aaagcaagat
     5821 agtttagcaa gtgtttattt tcttcttcta ttgatcgatt atcagatttt ccgctattgg
     5881 ggtgggggaa ttggctatac attgatgtat actacgttgt ttttgcttct attaagcgcg
     5941 cttctcttta gttacaaaat cttgcttcaa ctggaacaaa aagaagcttc ttggattact
     6001 gcatttttct tgttttttaa tgatttcaaa agcgcgcttt tctatttggc aggcacgttg
     6061 atccttcttg tcgcattctg gtttgcagga ccaatctatt ttgctttatt aggtttcagt
     6121 ttcttgtttt attttcaagt cctgcttttt atccatcgta cgcaagaaaa ggttcttaaa
     6181 aaggaggaat aacatgttgc gtttgattgc taaaatcgat gatagtatta tcaacaagtt
     6241 cgccaagcaa cggaaaacgg atagtcgaaa aatcaatttt ttgaaattta tcagtaatat
     6301 ctctaatggt ggttttattt ggttgctcct tgctgctgtt ttagtcttga tacctgatac
     6361 tagagaatat ggtctagcca ttcttatcgg cagtttgatc tatttttttg ttggtttgct
     6421 ttttatcaaa aaaacgacaa gacggaaaag accttatgaa aaaaatcgtc ggcttgtcct
     6481 ttacacaagt aaaccgaaga cttcttcttt tccttcagga catacagcgc tagccttttt
     6541 atcagtagct gcgttgttcg aattaagtcc agttgcagga atattagcct ttttatttgc
     6601 ttgtttgatc gcttattcga ggattttttt acttttacat tatcctactg acattttagg
     6661 aggagctatt ttaggaagtg ttattggcat cctatgcgct gccgttatct gaattaattg
     6721 tacgaataaa gcatgagtgc gtaaatgcgc atttgtgctt tatttttttg tttcgaggaa
     6781 agcgtagttg tttattcatt tgtcttaata act
//
LOCUS       DAYYBG010000120         6758 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000120 DAYYBG010000000
VERSION     DAYYBG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.123"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..625)
                     /locus_tag="ACHAUN_002567"
     CDS             complement(167..625)
                     /locus_tag="ACHAUN_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8461225.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(638..1969)
                     /locus_tag="ACHAUN_002568"
     CDS             complement(638..1969)
                     /locus_tag="ACHAUN_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8461226.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1980..3422)
                     /locus_tag="ACHAUN_002569"
     CDS             complement(1980..3422)
                     /locus_tag="ACHAUN_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8461227.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3409..4173)
                     /locus_tag="ACHAUN_002570"
     CDS             complement(3409..4173)
                     /locus_tag="ACHAUN_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8461228.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4277..5152)
                     /locus_tag="ACHAUN_002571"
     CDS             complement(4277..5152)
                     /locus_tag="ACHAUN_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8461229.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5407..5491)
                     /locus_tag="ACHAUN_002572"
     tRNA            complement(5407..5491)
                     /locus_tag="ACHAUN_002572"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5455..5457),aa:Leu,seq:caa)
     gene            complement(5509..5580)
                     /locus_tag="ACHAUN_002573"
     tRNA            complement(5509..5580)
                     /locus_tag="ACHAUN_002573"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5546..5548),aa:Gln,seq:ttg)
     gene            complement(5590..5662)
                     /locus_tag="ACHAUN_002574"
     tRNA            complement(5590..5662)
                     /locus_tag="ACHAUN_002574"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5627..5629),aa:His,seq:gtg)
     gene            complement(5724..5808)
                     /locus_tag="ACHAUN_002575"
     tRNA            complement(5724..5808)
                     /locus_tag="ACHAUN_002575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5772..5774),aa:Leu,seq:caa)
     gene            complement(5829..5899)
                     /locus_tag="ACHAUN_002576"
     tRNA            complement(5829..5899)
                     /locus_tag="ACHAUN_002576"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5865..5867),aa:Cys,seq:gca)
     gene            complement(5945..6016)
                     /locus_tag="ACHAUN_002577"
     tRNA            complement(5945..6016)
                     /locus_tag="ACHAUN_002577"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5982..5984),aa:Gln,seq:ttg)
     gene            complement(6028..6100)
                     /locus_tag="ACHAUN_002578"
     tRNA            complement(6028..6100)
                     /locus_tag="ACHAUN_002578"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6065..6067),aa:His,seq:gtg)
     gene            complement(6103..6176)
                     /locus_tag="ACHAUN_002579"
     tRNA            complement(6103..6176)
                     /locus_tag="ACHAUN_002579"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6142..6144),aa:Trp,seq:cca)
     gene            complement(6183..6263)
                     /locus_tag="ACHAUN_002580"
     tRNA            complement(6183..6263)
                     /locus_tag="ACHAUN_002580"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6227..6229),aa:Tyr,seq:gta)
     gene            complement(6265..6340)
                     /locus_tag="ACHAUN_002581"
     tRNA            complement(6265..6340)
                     /locus_tag="ACHAUN_002581"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6305..6307),aa:Phe,seq:gaa)
     gene            complement(6376..6448)
                     /locus_tag="ACHAUN_002582"
     tRNA            complement(6376..6448)
                     /locus_tag="ACHAUN_002582"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6413..6415),aa:Asp,seq:gtc)
     gene            complement(6458..6530)
                     /locus_tag="ACHAUN_002583"
     tRNA            complement(6458..6530)
                     /locus_tag="ACHAUN_002583"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6495..6497),aa:Val,seq:tac)
     gene            complement(6546..6617)
                     /locus_tag="ACHAUN_002584"
     tRNA            complement(6546..6617)
                     /locus_tag="ACHAUN_002584"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6582..6584),aa:Glu,seq:ttc)
     gene            complement(6632..6721)
                     /locus_tag="ACHAUN_002585"
     tRNA            complement(6632..6721)
                     /locus_tag="ACHAUN_002585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6685..6687),aa:Ser,seq:gga)
BASE COUNT         2051 a         1482 c         1093 g         2132 t
ORIGIN      
        1 tttaagaatc ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa ttcgataatt
       61 aacaaaaatc aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt
      121 aaattcaccc ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac
      181 aactatcttt aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg
      241 gagaaactac aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa
      301 tagctaacag tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac
      361 ttccttcgag ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac
      421 tttcgatcgt tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg
      481 catggatata tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa
      541 agacctcgtc ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa
      601 tgtttcttgt tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt
      661 ttttgtttac tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg
      721 tttgattcaa tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta
      781 ctagtaactt ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg
      841 gttttcttag tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa
      901 ctgcatctga ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac
      961 gacgatcatc attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa
     1021 aattaaagtt caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg
     1081 tatttttctc tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct
     1141 caacgaccat atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa
     1201 aatttttcgt gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat
     1261 taaaatcggc gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt
     1321 ctcgttgcaa ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat
     1381 acgtaacgat ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg
     1441 aaatagtcat atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt
     1501 cagaaacgat cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt
     1561 gtatatgatc gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc
     1621 cgagtccaca cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt
     1681 ctcgcatctc caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag
     1741 caagctttgg atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca
     1801 taaatttaac aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta
     1861 tttcagataa tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat
     1921 acgtactccc gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc
     1981 tattcttcta ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata
     2041 atttgataat ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc
     2101 tgttcctcga ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag
     2161 acatccagga tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca
     2221 taatttttac ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct
     2281 gccttagctt cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta
     2341 acgtaataca aagcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg
     2401 attcgaaaat tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata
     2461 ttgaataagt aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac
     2521 ataggagaaa gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa
     2581 gttcctgcac cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct
     2641 ttatagatag ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat
     2701 aaaaacagtc cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact
     2761 tccttgctag gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg
     2821 tgcagtccaa aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct
     2881 cccatatcaa cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata
     2941 aagcccaaaa aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt
     3001 tcgcccgcaa aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc
     3061 catgccgcta ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc
     3121 ataatttcta agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt
     3181 cctttgtcct tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag
     3241 tttccaaaaa cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct
     3301 gataaacgca tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt
     3361 ccaagcttct gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc
     3421 atatgttact aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat
     3481 taaaaattcg acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg
     3541 atccaaatac gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag
     3601 taaacttgcc aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc
     3661 tcctacgaca tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc
     3721 aaagttgctc cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt
     3781 ttcatccaaa gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg
     3841 ataaattttt tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa
     3901 tggatgctga taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt
     3961 tttgaggagt ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat
     4021 ttcctttctt agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga
     4081 tgtcaaaatt cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc
     4141 aaaatcatcc gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa
     4201 ataggcgcaa ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga
     4261 tagttacgcc cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat
     4321 tttcttgaat ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac
     4381 aatacgcttt gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt
     4441 cttcctgata aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag
     4501 tcagtgattc tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg
     4561 tcgtcatttc tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa
     4621 tgatatgcat agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat
     4681 tgattcgctc gaccacttct actgctatat catattcatt tggataaagg aatctcattt
     4741 ctggcaacat aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa
     4801 tatgatctgt caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta
     4861 tcagttcaat cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct
     4921 cactgaatcg tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa
     4981 catcaaatgg cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt
     5041 ctattccatc gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg
     5101 ttaattcacc tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt
     5161 ttgtctccaa acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc
     5221 gtgcctaatt gaatagtagc accctacata atttacaaaa atagtttaac acataataaa
     5281 agcgttatca atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga
     5341 ggtaattaca taaaaacaaa aaaaatatag caactctcta taagaaagtt gctatattga
     5401 taattatacc ggcggccggg gtcgaaccgg cacgtcctca acggacactg gattttgagt
     5461 ccagcgcgtc tgccaattcc gccacgccgg caaatttaaa aataataact ggggtagctg
     5521 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
     5581 agaataaaag gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg
     5641 ttaaccactt cgccacattc gccatcataa aagatgttat ttaatttctc aataaaaaac
     5701 taagatgaag acatcttagt caataccggc ggccggggtc gaaccggcac gccctcaacg
     5761 ggcactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa gttgatttat
     5821 tagtactaag gcggtaaccg gatttgaacc ggtgatgaag gttttgcaga cctctgcctt
     5881 accacttggc tataccgcca ttatactatt atcaatctat aaaataaaga ataaattata
     5941 gaaactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc
     6001 gcttggctat accccaagaa taaaaagggc gaatgatggg aatcgaaccc acgaatgccg
     6061 gagccacaat ccggtgcgtt aaccacttcg ccacattcgc catggcaggg gcagcaggaa
     6121 ttgaacccac actaacggtt ttggagaccg ctgttctacc tttaaactat gcccctataa
     6181 aatggaggag agtggattcg aaccactgaa ccctaaggaa cggatttaca gtccgtcgcg
     6241 tttagccact tcgctactcc tccatggtgg cgcgggacag aatcgaactg ccgacacacg
     6301 gagcttcaat ccgttgctct accaactgag ctaccgcgcc atattttaaa attattaatt
     6361 gaataacaag aacgacggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca
     6421 ggcatgttaa cccctacacc acggaaccag ttcattatgg aggttaacgg gatcgaaccg
     6481 ctgaccctct gcttgtaagg cagatgctct cccagctgag ctaaacctcc aacattcaat
     6541 taaaatgacc cgtacgggat tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac
     6601 cgcttgacca acgggccagg taaatttgct acggagagta agggattcga acccttgaga
     6661 cagtttggca ccatctacat gatttccaat catgctcctt cggcctctcg gacaactctc
     6721 cagagtgaaa gaagctcaat actctttctc aaaatgag
//
LOCUS       DAYYBG010000121         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000121 DAYYBG010000000
VERSION     DAYYBG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.124"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHAUN_002586"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHAUN_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHAUN_002587"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHAUN_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8461230.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHAUN_002588"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHAUN_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHAUN_002589"
     CDS             complement(2143..2373)
                     /locus_tag="ACHAUN_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8461231.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHAUN_002590"
     CDS             complement(2603..3421)
                     /locus_tag="ACHAUN_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8461232.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHAUN_002591"
     CDS             3582..4271
                     /locus_tag="ACHAUN_002591"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8461233.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHAUN_002592"
     CDS             4285..5787
                     /locus_tag="ACHAUN_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8461234.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHAUN_002593"
     CDS             5800..6276
                     /locus_tag="ACHAUN_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8461235.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHAUN_002594"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHAUN_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYBG010000122         6324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000122 DAYYBG010000000
VERSION     DAYYBG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.125"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHAUN_002595"
     CDS             complement(99..1229)
                     /locus_tag="ACHAUN_002595"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8461236.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHAUN_002596"
     CDS             complement(1878..3080)
                     /locus_tag="ACHAUN_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8461237.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHAUN_002597"
     CDS             complement(3140..3343)
                     /locus_tag="ACHAUN_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8461238.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHAUN_002598"
     CDS             complement(3485..4552)
                     /locus_tag="ACHAUN_002598"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8461239.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHAUN_002599"
     CDS             complement(4610..4717)
                     /locus_tag="ACHAUN_002599"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461240.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHAUN_002600"
     CDS             4880..5692
                     /locus_tag="ACHAUN_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461241.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHAUN_002601"
     CDS             complement(6066..6257)
                     /locus_tag="ACHAUN_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461242.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1881 a         1299 c          997 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaa
//
LOCUS       DAYYBG010000123         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000123 DAYYBG010000000
VERSION     DAYYBG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.126"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHAUN_002602"
     CDS             725..811
                     /locus_tag="ACHAUN_002602"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8461243.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHAUN_002603"
     CDS             complement(932..1621)
                     /locus_tag="ACHAUN_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461244.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACHAUN_002604"
     CDS             complement(1628..1906)
                     /locus_tag="ACHAUN_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461245.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACHAUN_002605"
     CDS             complement(1866..2780)
                     /locus_tag="ACHAUN_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8461246.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHAUN_002606"
     CDS             complement(2762..3142)
                     /locus_tag="ACHAUN_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8461247.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHAUN_002607"
     CDS             complement(3592..3879)
                     /locus_tag="ACHAUN_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8461248.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHAUN_002608"
     CDS             complement(3899..4123)
                     /locus_tag="ACHAUN_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8461249.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHAUN_002609"
     CDS             complement(4578..4748)
                     /locus_tag="ACHAUN_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461250.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHAUN_002610"
     CDS             complement(4763..5296)
                     /locus_tag="ACHAUN_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8461251.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHAUN_002611"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHAUN_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8461252.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1634 a         1152 c         1041 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtacatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYBG010000124         5954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000124 DAYYBG010000000
VERSION     DAYYBG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.127"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHAUN_002612"
     CDS             <1..813
                     /locus_tag="ACHAUN_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8461253.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHAUN_002613"
     CDS             786..1229
                     /locus_tag="ACHAUN_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461254.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="ACHAUN_002614"
     CDS             2399..3367
                     /locus_tag="ACHAUN_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8461255.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="ACHAUN_002615"
     CDS             3384..3908
                     /locus_tag="ACHAUN_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8461256.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="ACHAUN_002616"
     CDS             4284..4565
                     /locus_tag="ACHAUN_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461257.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="ACHAUN_002617"
     CDS             4567..4983
                     /locus_tag="ACHAUN_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461258.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="ACHAUN_002618"
     CDS             5613..>5954
                     /locus_tag="ACHAUN_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM8461259.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2259 a         1017 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggcatagtgt acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DAYYBG010000125         5904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000125 DAYYBG010000000
VERSION     DAYYBG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.128"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(223..1005)
                     /locus_tag="ACHAUN_002619"
     CDS             complement(223..1005)
                     /locus_tag="ACHAUN_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461260.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1070..1906)
                     /locus_tag="ACHAUN_002620"
     CDS             complement(1070..1906)
                     /locus_tag="ACHAUN_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8461261.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1924..3333)
                     /locus_tag="ACHAUN_002621"
     CDS             complement(1924..3333)
                     /locus_tag="ACHAUN_002621"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8461262.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3347..5170)
                     /locus_tag="ACHAUN_002622"
     CDS             complement(3347..5170)
                     /locus_tag="ACHAUN_002622"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8461263.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5722..>5904)
                     /locus_tag="ACHAUN_002623"
     CDS             complement(5722..>5904)
                     /locus_tag="ACHAUN_002623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461264.1"
                     /translation="NITMIVGNFYVIHCYNNIKEMKKKKLLWICLVFLLIIGGIEILT
                     TCRLEINQFKHLLVNF"
BASE COUNT         1817 a         1278 c          953 g         1856 t
ORIGIN      
        1 aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt
       61 aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga
      121 aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata
      181 acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt
      241 aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatctttttt
      301 cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact
      361 gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt
      421 atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc
      481 tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc
      541 tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc
      601 tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt
      661 agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc
      721 aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag
      781 cattttgttg tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg
      841 gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc
      901 tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt
      961 agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt
     1021 agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc
     1081 ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt
     1141 cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca
     1201 ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa
     1261 taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt
     1321 tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca
     1381 tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc
     1441 cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc
     1501 agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa
     1561 aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa
     1621 atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga
     1681 cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag
     1741 catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc
     1801 ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat
     1861 ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc
     1921 tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt
     1981 tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg
     2041 cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa
     2101 aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc
     2161 aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc
     2221 attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg
     2281 tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc
     2341 tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac
     2401 atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat
     2461 aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa
     2521 tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc
     2581 taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat cttctggact
     2641 ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt
     2701 caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg
     2761 aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg
     2821 aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc
     2881 agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc
     2941 gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat
     3001 tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt
     3061 tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa
     3121 gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac
     3181 ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc
     3241 atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt
     3301 ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca
     3361 tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac
     3421 tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat
     3481 cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa
     3541 agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt
     3601 ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa
     3661 caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct
     3721 tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt
     3781 caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc
     3841 caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt
     3901 gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa
     3961 caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa
     4021 tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag
     4081 taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt
     4141 tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc
     4201 ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca
     4261 ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa
     4321 ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca
     4381 acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca
     4441 tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat
     4501 aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta
     4561 ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg
     4621 gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg
     4681 ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca
     4741 aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa
     4801 tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa
     4861 tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa
     4921 ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca
     4981 ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg
     5041 ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg
     5101 ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc
     5161 tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca
     5221 atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata
     5281 acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt
     5341 aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta
     5401 gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta
     5461 aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat
     5521 ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag
     5581 taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt
     5641 tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcgata
     5701 gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc
     5761 taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat
     5821 ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa taacataaaa
     5881 attcccgaca atcattgtaa tatt
//
LOCUS       DAYYBG010000126         5868 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000126 DAYYBG010000000
VERSION     DAYYBG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.129"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..684)
                     /locus_tag="ACHAUN_002624"
     CDS             complement(37..684)
                     /locus_tag="ACHAUN_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8461265.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(692..1378)
                     /locus_tag="ACHAUN_002625"
     CDS             complement(692..1378)
                     /locus_tag="ACHAUN_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8461266.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1384..2457)
                     /locus_tag="ACHAUN_002626"
     CDS             complement(1384..2457)
                     /locus_tag="ACHAUN_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8461267.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2679..3353
                     /locus_tag="ACHAUN_002627"
     CDS             2679..3353
                     /locus_tag="ACHAUN_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8461268.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3358..4698
                     /locus_tag="ACHAUN_002628"
     CDS             3358..4698
                     /locus_tag="ACHAUN_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8461269.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4847..5833)
                     /locus_tag="ACHAUN_002629"
     CDS             complement(4847..5833)
                     /locus_tag="ACHAUN_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8461270.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         1771 a         1123 c         1038 g         1936 t
ORIGIN      
        1 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
       61 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
      121 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
      181 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
      241 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
      301 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
      361 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
      421 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
      481 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
      541 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
      601 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
      661 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
      721 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
      781 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
      841 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
      901 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
      961 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
     1021 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
     1081 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
     1141 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
     1201 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
     1261 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
     1321 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
     1381 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
     1441 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
     1501 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
     1561 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
     1621 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
     1681 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
     1741 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
     1801 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
     1861 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
     1921 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
     1981 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
     2041 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
     2101 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
     2161 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
     2221 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
     2281 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
     2341 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
     2401 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
     2461 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
     2521 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
     2581 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
     2641 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
     2701 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
     2761 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
     2821 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
     2881 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
     2941 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
     3001 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
     3061 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
     3121 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
     3181 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
     3241 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
     3301 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
     3361 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
     3421 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
     3481 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
     3541 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
     3601 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
     3661 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
     3721 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
     3781 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
     3841 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
     3901 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
     3961 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
     4021 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
     4081 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
     4141 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
     4201 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
     4261 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
     4321 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
     4381 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
     4441 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
     4501 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
     4561 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
     4621 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
     4681 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
     4741 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
     4801 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
     4861 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
     4921 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
     4981 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
     5041 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
     5101 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
     5161 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
     5221 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
     5281 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
     5341 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
     5401 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
     5461 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
     5521 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
     5581 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
     5641 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
     5701 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
     5761 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
     5821 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggt
//
LOCUS       DAYYBG010000127         5689 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000127 DAYYBG010000000
VERSION     DAYYBG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.131"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACHAUN_002630"
     CDS             complement(488..1297)
                     /locus_tag="ACHAUN_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8461271.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACHAUN_002631"
     CDS             complement(1297..2043)
                     /locus_tag="ACHAUN_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8461272.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACHAUN_002632"
     CDS             complement(2061..2528)
                     /locus_tag="ACHAUN_002632"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8461273.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACHAUN_002633"
     CDS             complement(2528..2938)
                     /locus_tag="ACHAUN_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8461274.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACHAUN_002634"
     CDS             complement(2949..3962)
                     /locus_tag="ACHAUN_002634"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8461275.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACHAUN_002635"
     CDS             4179..4913
                     /locus_tag="ACHAUN_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8461276.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACHAUN_002636"
     CDS             4897..5607
                     /locus_tag="ACHAUN_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8461277.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAYYBG010000128         5633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000128 DAYYBG010000000
VERSION     DAYYBG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.132"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..2187
                     /locus_tag="ACHAUN_002637"
     CDS             34..2187
                     /locus_tag="ACHAUN_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFM8461278.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2156..3571
                     /locus_tag="ACHAUN_002638"
     CDS             2156..3571
                     /locus_tag="ACHAUN_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFM8461279.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3604..4638
                     /gene="sppA"
                     /locus_tag="ACHAUN_002639"
     CDS             3604..4638
                     /gene="sppA"
                     /locus_tag="ACHAUN_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8461280.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4640..5161
                     /locus_tag="ACHAUN_002640"
     CDS             4640..5161
                     /locus_tag="ACHAUN_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8461281.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5158..5544
                     /locus_tag="ACHAUN_002641"
     CDS             5158..5544
                     /locus_tag="ACHAUN_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8461282.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2021 a          765 c         1070 g         1777 t
ORIGIN      
        1 taaaaatttt ttagaaggag ttgtgttaga taagtgataa aatcatttcc ttttgtgcag
       61 caacaagatg aaaaagattg tggtgttgct tgcctttcga tgattttaaa atattttaaa
      121 actgatatcc cgattcataa attacgggac ctatctggga ctgatttaga gggtacttca
      181 gcatttggat tgaaaaagac atttgaaaaa ttgtcttttg attgtatggc tattcaagca
      241 gatagtgaga tctggaaaga aagagatctt atcttaccat taattgcaca tatattggtt
      301 gataacagct atatgcacta cgttgtaatt tataaaacga agggaaacgt actatggatt
      361 gcagatcccg ctaatggaaa aaatagaaaa acgattgaag cgtttcaaaa agaatggaca
      421 ggtattcttt tattacctat gcctagagaa acttatattc ctattaaaga aaaagtagct
      481 ggattaagtt cttttttgcc tataatttgg aaacaaaaag gattagtatt ccacatcgtt
      541 ttagcatcga ttttcattac gttttttggc ataggaagct catattattt tcaaggtatt
      601 cttgattttt tcatccctaa tcaagcacgt tcaactttaa atataatttc cattggcttg
      661 atcgctgtct atttatttcg ggtattgttt gaatatagtc gtagttatct tcttattatt
      721 ttaggacagc gaatgagtat ggcaattatg cttcagtatt tcaaacatgt gttaacttta
      781 ccaatgaatt tttttgctac gagaaaatca ggggaaatta tttctcgttt tcttgatgcc
      841 agtaagatta ttgatgcttt agcaagtgct acgttatcaa tatttttaga tattggtatg
      901 gtgttgcttg ttggggttac gttagctatt cagaatagta cgttgttttt aatcactcta
      961 tgctctttgc ctttttatgt agttgcaata ttcgctttcg tgaaaagcta tgaaaaatcg
     1021 aacagggaag aaatggcagc cggtgcagct gtaaattcta gtatcataga aagtcttaaa
     1081 ggaatagaaa caattaaagc atatagcggt gaagagaaag tatataaccg tatagaccat
     1141 gaatttgtta aattaatgaa aaaatcattt cgtacggtta cgcttgataa tattcaacaa
     1201 ggaattaaac atttgattca attaattagc agtgcattaa ttttatggct aggttcttat
     1261 tttgttatgg ataatagaat tagtctaggt caacttatca catacaatgc tttactagta
     1321 tttttgacag atcctttaca aaacattatt aatttacaag tgaaaatgca aacggctcaa
     1381 gttgcaaata atcgtttaaa tgaaattttc gcgattgatt cagaaaaatc gaaccagtct
     1441 tttgaatata tgctttctga acaattattt caacaagata tcactgtaaa aaacgtttca
     1501 ttctcgtata gcatgaaggc cccaacgttg aacgaaattt catgtacaat tccttcgcac
     1561 agtaaaacgg ctatagttgg agttagtggt tctggaaaat caactcttgc aaagttattt
     1621 gttcgttttt atgaagcatc agaaggatcg atccattatg gaaacattaa tcacttagac
     1681 attccttttc agatattacg cgatcgagtg acatatttgc ctcaagagtc atttttcttt
     1741 agtggaacaa tcattgagaa tcttatattc ggtttagata gcccaccaag tttcgaacaa
     1801 ataatagaag tttgtgagat agtccaacta aatgaattta ttaatcaaca gccattacgt
     1861 tacgagacat tgttggaaga aggtggaaat aatctttcag gagggcaacg acaacgatta
     1921 gccttagcac gggctttatt aaaaaatccg gacatattaa ttttagatga agcaactagt
     1981 ggattagata ccttgttaga acatgcaatt atgaattatc tcctcaattt aaaagaaaaa
     2041 acgattattt ttattgcgca tcatttacca atagcaaaag catgtgatca aattttagtt
     2101 ctccatgaag ggcatttggt agaaaaagga acgcacgatg aacttcgttt tgccgatggt
     2161 atctatcaaa aattatggga aatctaggag agggaaacaa tttatgcaga ataataattg
     2221 gttcgaacat tctagtatct atactaaaca acatcatagc ttctaccgtt gggtgttata
     2281 ccctgtaatt ctttttttat gtattattgg attgttttta acctttgcta aaaaagaggt
     2341 tgttattcgg acaacggctc aattgaaccc cgaaaaaata gagaagttac aagtgccatt
     2401 agaagctaaa ataatggaaa acttcctaaa agaaaatcaa tttgttcata aggacgatgt
     2461 gttggttaga ttggattgtt ctttaataga gaatgaaaag gctcagatag aacaagaaaa
     2521 ccaacgtatt actcaacaaa taaagatggc tcagctattt attgaaagta taagtaaagg
     2581 aaaaaatttg ttttcaacgg atgacagttt tggctacagt aatcaattaa agagcatgtt
     2641 gtcagaaaaa gaatcactcc gctacgcttt gaagcaaagt gaattaaatg atcaaaagca
     2701 attagaagta tacgaaaaga caaaaagaca actagaaaaa caaattgaga gttcagatag
     2761 taaattacaa gaatggcaac aagtacaggt agcttggagt aataatcaat cattaaaaga
     2821 tttttcaaaa gaaatgatgg caaactatga gaattggcaa gaacaactaa ataatgtttc
     2881 tgatgatcaa aaaaatcaag tgaaactgac aatttcagca agcataaatg aacaaattga
     2941 gcaactaaaa aaagaagtag aacagtatca gtcagaaaaa gctaaattag ttaaaccaac
     3001 tacttctgag aatgacagaa ttagtcaaac ggaaaaagga aagcaagagc tagaacaagc
     3061 aataacaact gtaaaacaaa cagttacaga gctaaatgaa aaacaagaga agaaccagtc
     3121 agttatcaaa agtttggacg atcaactttc aaaaggaata ctaaaagctc ctgtatctgg
     3181 aacggttcat ttaaatgaag aaacaaaggg acaagcagaa ctagccaaag gaacagtgct
     3241 tgcagaaatt tatccgatcc acgaaaaatc aaaaatgaaa tttacagcac ttttacctgc
     3301 gaatgaaagt acctatatta aagaagggat gaaggttcat tttaaattgg atcaaaaagg
     3361 tggggctccg attacgatcg acggaacttt agatgaaatt tcagaaaatt ctactgccac
     3421 tgagaaaggt gttttttatg cagtgaaagg aacgttaaaa acaactcaat cgtttccgtt
     3481 tcgttatgga ttaactggag agttgtcttt aattgtagga gaaaaaacat attggcaaca
     3541 aattgtcgat gtgtttctaa ataaaaaata attattttta ctaagaatat aggaggagtt
     3601 tatatgaata aaagaagatg gattgctgtg ggagtagcgt gcggattatt gttactatcc
     3661 attatcgtcg cgctaattcc aggaaaagat aaagaggaag catcaaatac aaccctaaca
     3721 ggaataaata agatttttta cggatctaac gaaatcacag aagaaacgtt agaagaaggc
     3781 gcatcaaata aaaagattgt caaactatcc gtcaatggag ttattgcaga tacaggtgaa
     3841 agcaatcttt ttagtaggga acaatataac catcagaatt ttttaacaca gctaaagaaa
     3901 attcaagaag acaaagcggt taagggagtg ttattagaag taaattcacc tgggggagga
     3961 atatatgaaa gtgctgagat tgctaaagaa atggcaaaaa ttaaaaagct agatattcct
     4021 atctatactg catttaaaaa tacagcggca agtggagggt actacatttc tgctggttca
     4081 gataaaatat ttgctacgga agaaacaaca actggatcaa ttggtgtcat tatttctggt
     4141 ttaaattatt ctggcttatt agaaaaactt ggcgtaactg atgcgacgta taaaagtggt
     4201 gcattaaaag atatgatgtc acctcaacac aaaccttcag aggaagagaa taaagttata
     4261 caggaatttg tgatgagtgc ttatgatcgt tttgtcaatg ttgtagcaaa aggaagaaat
     4321 atggatacta atgcagtgaa agaacttgcg gatggaagaa tttatgatgg taaccaagca
     4381 gtagaaaatg ggttggtgga tcaaattggt tactctgaag atgcattaga ttccctaaaa
     4441 aaagagaaga aattaactga tgcgacggtt atagaatata aaaatgacac aactggcttt
     4501 gcttcttctt ggttaggtaa taagattgct gaatggcaag ggctaaaggc aactacatca
     4561 gatcgtgtaa tttcagtttt tgaaaaactt ggattttctg atactccaaa accaatgtat
     4621 tactatggag ggctataaaa tgaataagaa gagtggcatt gaaactttgc ctaattatgt
     4681 atttgcggga ttttggttac gattttttgc ttttcttatt gatttaattt gtattagcat
     4741 catcacccat gctactatag gtctctttta caaaatcatg ggactggaat ctgacaaatc
     4801 cattattact ttttatggta tagcgtcatt agctatttat ttaacctatt ttgtactttt
     4861 gactaagctg aattcaggac aaacaattgg gaaaatgatt tttgggataa gagtagtgag
     4921 ctttgaggaa aaagagctaa gttggcaaac tatctttatt cgtgagtgta tatgtaggtt
     4981 tatattaaaa aattttgtat ttcaaattgg gtatttggtg gctgctttca cgcccaagaa
     5041 acaacatatt ggggattatt tttctaacac gtcagtggtt gttattcgtt cattagaagc
     5101 tattaactat ggtcaaaaaa tttctaattc taaaacactt aatctggaga gaattaaatg
     5161 aataagagaa aaacagagtt tattttgatt ttaattagtg gaataacagg gattttatgt
     5221 agtcttttag cattgatggg tggtatgttt ctatcttcta ttgattcaac tactttaaat
     5281 actacagaac taaccagtaa ggatatagat atgttagtca atctaagcgg gaatgttgtt
     5341 ttatttggag gaattaccct agttttttcg ataatgattt tcataatagg ttttttagta
     5401 agaaaaaatc aaaaaacagt ggtctatggt attagtttac tcattttatc tattattcca
     5461 ttttttcttt taacattatt gtgggtaatt cctggagttt taggtattat tgcgggattg
     5521 atgcttctaa taagaaaatc ttaattaggc aatgtgtaga caacgtgaaa cgattaatca
     5581 attaaaagct aaatacgttt tgagaaatga taacttgtag tcgatggaag aca
//
LOCUS       DAYYBG010000129         5554 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000129 DAYYBG010000000
VERSION     DAYYBG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.133"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACHAUN_002642"
     CDS             <1..377
                     /locus_tag="ACHAUN_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFM8461283.1"
                     /translation="KDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEI
                     VPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKA
                     DYVTVSNDKDGVSQAFERILTI"
     gene            658..1878
                     /locus_tag="ACHAUN_002643"
     CDS             658..1878
                     /locus_tag="ACHAUN_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8461284.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1875..4595
                     /locus_tag="ACHAUN_002644"
     CDS             1875..4595
                     /locus_tag="ACHAUN_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8461285.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4579..5523
                     /locus_tag="ACHAUN_002645"
     CDS             4579..5523
                     /locus_tag="ACHAUN_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8461286.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2068 a          893 c         1095 g         1498 t
ORIGIN      
        1 aaaaggatgc tatcagcatt tacaaatttt ttatttttat tcctaatcgt caattgaaag
       61 ctaatatatt taaccatttg aaaaagatgc taaatgtgtc tgttactgaa tcggcacctg
      121 taaatattga aatcgtacct caaaatgtat ctaaaggtgt ggtgtttgat cgtttggaat
      181 caatctacaa tttaaagaaa cctttgagaa tcgcgatcgg agatagttta aatgacttat
      241 ctatgtttga aagtgcagat attagttttg caatgggaaa tagtcatcaa gaaatcatga
      301 aaaaagctga ttatgtcact gtttcaaatg ataaagacgg agtatctcaa gcatttgaaa
      361 gaattcttac catataaact aattaaggta gtatagaaca tagaattcta aatacggaaa
      421 aatggagcat aaattccagt acaaatagta cttcttcagt gtcaaacgaa gcgaatataa
      481 catagatatt taggattgtg acaaaaatca tgtcacaatc tttttttcga ataaacttat
      541 tttttgaagt cggccattta tcgtaatttc tttgcataat acgaacaaat tttcggttat
      601 tgatgaaaaa aaacattgaa aatggtatga taaaagaaaa aaaggaggaa atttttaatg
      661 gtgaaattta tccatgcggc agatttacat atggatcggt catttgaagg actgacaact
      721 cttgataaaa ctgtacagga aaaactattg aagacgaatc tgaccgtttt atcaaatatc
      781 attgaacagg caataataaa taacgttgat tttgttttac ttgcaggaga taattttcat
      841 caaaatcgtc cttcattaaa aatacagaaa catttttcag aacagatgaa acggttagaa
      901 aagaaccata tacctgtatt tataatattc ggaaatcatg atttctatca aaaagagcgg
      961 tattggttct cctttcctaa aaatgtccat ttatttacat ccgaaacggt agagacaaaa
     1021 aaaataacga taaaaagtgg agaaactgtc tctctcagtg gatttagtta tcgtcaaccg
     1081 tggattcaaa aagacaaagt gatggaattt ccttctagag aactcacaga ttaccatatt
     1141 gggttgtacc acggggaacc tggcgtctct caaaaaggaa actatgctcc tttccaaccc
     1201 tcaaagatgc aagaaaaggg atacgattat tgggctttag ggcatatcca tgtgccaacg
     1261 gtcttaaatg aaaagcaaac aattgtttat ccaggtgcgc ctcaaggtca tactaaaaag
     1321 gaacaagctt ctacatctat tctactagtt gaattaagta aaggaagttg ccaaatacat
     1381 ccgattaaag tagcagaggt ctattggaag acaaaggaaa tatcattgag gaaagcaaga
     1441 acgacaaaag aggtcattgt tcacattcgt cagcagcttt caaaagtaga ggatggattt
     1501 agtttaattg agataaaatt aaaagattat gaccatctca atacagatgt tttggaacgg
     1561 atacaatcag gagagctatt agactatttg ttagaggaat tctcagatag gataaatgat
     1621 ttctttattt ggcgtatttc tctcatagag aatacttttt atacgaagac cccacttgct
     1681 gcttctgcta aattgatgga acagttattt caatactatc agacacctca agatttccaa
     1741 caaattttaa atgaagtata tagtcatcaa gaagcggcta gaatattgag cgaactgccg
     1801 gaatatcaag aagaaacact tgaaaaagcc aagcagctgc ttaatcaaga ttttttattt
     1861 gaggaggatc aggaatgaag attttaagga tcgagattgc tgcttttgga aaatggcgtc
     1921 aaaaaagttt tgacttttat tcaggcaatc agctgatcta tgggggaaac gaagcaggaa
     1981 aatcaacgat ttatcaattt attcaagcaa tattatttgg tttcccttca aaaggccgta
     2041 aaaaaaagga ttatacacca aaagacggct cagcctatgg cggcaaaata tggcttaaac
     2101 atcctgtata tggtgaattt gctgtcgagc gctacaagca gcaaaatcgc ggaaaatcga
     2161 aggtgtggct gggggatcaa gtgggaagtg atgagttact agaaaaactg atcgcacctt
     2221 tgacgaaaga gttattccag caggtattta ctttccagca agagcagttg atggaactag
     2281 atcatttgag agaaaaagag ctacatgatg cgctgatttc tttaggcatc acgggcagtt
     2341 ctcaagtatt ccaaaagcgg caagaatatt atcagaaagc acaacaatta ttcaagaaaa
     2401 aaggccaaaa attactaatc aatcaaaaat taaatgaatg gaaagagctg caagaaaaaa
     2461 ttcatcaaaa acaagaacag gaagcttatt ttggcaagtt gatccaacaa gaacaggaat
     2521 acgagtcgca acagcagcag ttattggcac aattaaaaga ggcgaacaaa caattttcat
     2581 tagccagaca gcaagaactt aatttttctt tatatgaaga atggcaatct ctcaaaaaaa
     2641 acagcactac aggtcttcct gatgagcaac ttcttttaga tctggagtct ttctccaaac
     2701 ggtatcagca aacaaatgag cgtttggaag aattagaagc aacactggaa aagcatagcg
     2761 ggatggatca gcagtcagcc agatattatt tttatttaga gcaggagcag gaactaaaag
     2821 atctccagca acttcaattt acagcggaac aattaaacga agaatggcaa agattacaaa
     2881 ttgagactag agaaacaaat tcagaattga agattttgga acaaaggtgg cattggcaga
     2941 aaaatccgtt accggaagaa tttttatatg aagaagaatg gaagcatctt ctggctgaag
     3001 aaagaaggat tgctgatcta gaaagccaga tttctgtgcg tctccaaata gctaaagaac
     3061 agcaagagat gattgaagca gaagtgaatg acttggaaga gacctatcca gcattattac
     3121 aaaatcagtc agagatagca ccagtctctt catctaaacc gtatatgctg atggctggcg
     3181 gagcaataat cattgcaggt atatttgtac caatgccctt gaaagtgatc cttttactaa
     3241 ttggtgcagg catgattggc tatttcttct acaaaaggca accaaaaatt aaaactaaaa
     3301 atgatttctc tgagataaaa ggaatgtggc aagaaaaact aggacaactt gatctagcct
     3361 tagctcaatt agctgaagca gaagaaaaac aaaagagaac tggagaagaa aaacagattc
     3421 ttcagcaaga agtatatcgt tttgcggatc atcggcactt aggaaagatg gatacgctca
     3481 aactcatgaa agaatatgga caggaagtca tagattatca gaaattatat ggcgtatttg
     3541 agcggaaaca taaaagagaa caggaaatac aagaaaaact acgacaaatc gaccaaaaat
     3601 ttgagtttgt taaagaatgg ttaccgatac aagataagac aatttcagaa aaaatggtat
     3661 tgctttcccg ttttatacaa gagatggaac agatgaaatt tgccagaagc tatcagcaaa
     3721 atactttatt gaagcaagaa atcaatcaat tgaaaaagaa acaaaaagta tggatacaag
     3781 aatacagcga acaattgact caagcaggct tatcttatcc ttcggaagta cctgtctttt
     3841 tacaaaaagc caaacaagcc gaacagcaga tgaaaagata caatgaatta tcggaaatgc
     3901 ttactccact atttcctgaa gagctgacaa aagagaaatt atctgtaaga ctgcgccaac
     3961 tgaaagaaaa gcaatcccaa ctccagcaag aagaaaaaga agcaagtgaa aatcggcaac
     4021 gactccaact tcaagtagaa gagttgcaaa gagacggcac attggacgaa ctttatcaac
     4081 aagctagccg gcaaaaagca aaattacagg aattactgga ggaatgggga gcacttgaaa
     4141 tcgccgggac attcttaggt gatctttcaa cagagatgag tgaaagacaa ttaccccagt
     4201 tgttaagagc agcaggtgat tatttaggta ttttaacaaa aggaaattat cagaagatat
     4261 tactgaaaaa tgatacactg actttaacag acggatatac agaatttccc atctatgagt
     4321 tatctacagg tacgaaagat cagctgatta tggcgatgag atttgcttac ctctcattag
     4381 aagctaaccg ttcgatatgc ccgatcatca ttgatgatgg ctggcttcat tatgatcatc
     4441 agcgaaagta tcaattagct gaacttttag ctaaatttgg agaaaagtat caaataattt
     4501 gtttttcttc tgataaagaa atggtaagct attaccatga gatgaaccaa atcgtaaaac
     4561 aattagaagg agcaaataat gaaaaaaatt cgtgatttag taatcgatga gcagtttgaa
     4621 caatttgtcc tgatcaaaag tgcagatgta agaatcgcta aaaacggaaa aaaatttatt
     4681 gcttttactt tccaagatac ttcaggtact atcgatggta aatactggga tgcttcggag
     4741 gaggaaatca ataaatttac agccggcaca gtcgtttttc taaacggtaa aagagaggtt
     4801 tatcaaggaa accctcaagt aaagattctt catatgcgct taacaagggt agatgagcca
     4861 ggagagccta gtttatacat ggaaagagcc cctttgaaga aagaagaaat ggaagaagaa
     4921 atcaatcaat tgttgtttga gatcaccaat gcgcattgga atcgtattgt tcgctatcta
     4981 cttaataaat atcaaaaaca attttttgag tttccggcag caaaaagaaa ccatcatgca
     5041 tttgccagtg gattagctta tcatactttg acaatgcttc acttagggaa atcaatcgcc
     5101 gatgaatatc ctgagctgaa tcgagcattg ttgtatgctg gtatcatttt gcatgattta
     5161 ggaaaggtga ttgaactttc cggtcctatg tcgacagaat atacgcttgc aggaaatttg
     5221 atcggtcatc tggtattgat tgacgaagaa ataactaaag cctgtcttgc gttgaaaatc
     5281 agcgaagctg acgaagatgt tctcgtatta cgtcatatgg ttttagcaca ccatggctta
     5341 ttagaatatg gctcacctgt acgtccaaga ataatggaag cagaaatatt acatcaaatc
     5401 gacaatatcg atgcttcgat gcagatgatg ttgacttcga ttcgtcaaac agaaccagga
     5461 aaatacaccg atcggatttt cggtatggac aaccgaagtt tctatgtacc aaaagattta
     5521 tagcaaaaat ttattttttt cattcaaaac aaaa
//
LOCUS       DAYYBG010000130         5481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000130 DAYYBG010000000
VERSION     DAYYBG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.134"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACHAUN_002646"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACHAUN_002646"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACHAUN_002647"
     CDS             complement(580..1353)
                     /locus_tag="ACHAUN_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8461287.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACHAUN_002648"
     CDS             1497..1847
                     /locus_tag="ACHAUN_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8461288.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACHAUN_002649"
     CDS             1828..2544
                     /locus_tag="ACHAUN_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8461289.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACHAUN_002650"
     CDS             2544..3992
                     /locus_tag="ACHAUN_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8461290.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACHAUN_002651"
     CDS             complement(4062..4571)
                     /locus_tag="ACHAUN_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461291.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACHAUN_002652"
     CDS             complement(4561..5286)
                     /locus_tag="ACHAUN_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461292.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAYYBG010000131         5286 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000131 DAYYBG010000000
VERSION     DAYYBG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.135"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..286)
                     /gene="prgO"
                     /locus_tag="ACHAUN_002653"
     CDS             complement(11..286)
                     /gene="prgO"
                     /locus_tag="ACHAUN_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8461293.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(258..1211)
                     /locus_tag="ACHAUN_002654"
     CDS             complement(258..1211)
                     /locus_tag="ACHAUN_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8461294.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1823..3316
                     /locus_tag="ACHAUN_002655"
     CDS             1823..3316
                     /locus_tag="ACHAUN_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8461295.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3451..3747
                     /locus_tag="ACHAUN_002656"
     CDS             3451..3747
                     /locus_tag="ACHAUN_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8461296.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3848..4699
                     /locus_tag="ACHAUN_002657"
     CDS             3848..4699
                     /locus_tag="ACHAUN_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461297.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4720..4932
                     /locus_tag="ACHAUN_002658"
     CDS             4720..4932
                     /locus_tag="ACHAUN_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461298.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1803 a          822 c          997 g         1664 t
ORIGIN      
        1 ttactatatt ttaatctttt gatcgtctat tatatataat ccgttcctcg tcgtttaaag
       61 tatcaacata ggcgtccatt gcaagtttga ctatttcata cataggaata tcttttgcat
      121 aactaagatc ccgaatggtt tttagtacgg gaggatctac ttgaactggt tttctttctt
      181 tcgcagtgta ctttcgttcc cctaactcga cattaatatc atctttaatt gaaatatctt
      241 cagcaccaat tttcttctta tctttgttcg ttggtaataa tgccatttag tttaatctcc
      301 tttccaagag gtaggatatg attggccatt gagtcagcat atttaggtgt ataatcaaat
      361 ccttcaatat caccatgttg ttcatagtat tcaattcttt cttctaattc tgtaaagatg
      421 tctgcaaaaa tagcccacca tcttcgatct acataatcat ttaattggat accattctca
      481 ccatacattt ctaaccgatc cgagccttta acaatgcttt taaaaatgtt tgattcacca
      541 tacttatcaa ttgttttttg atagtttgcg tgttgagagg aacgtcgcac tgagaaaaga
      601 acaggcaaaa ttccaatgat ttgaagatga gactcttcaa aattcgtgac aatcggaact
      661 aaaactttat taataaaatc ttctgttcct tccattgcgt accgttttaa ttcttgcatt
      721 gcaataatat aatctgcagc agttaagaat gcacgaacca cattatctgt tgatggtggg
      781 cagtcgataa agatataatc gaaatttttt ctcaattctt ctaaaggttt aatgaaggcc
      841 aatgatctct tacgacgatc aggatatttt tcggacaagt attcattaag ttcttcagat
      901 ccaggagagc cagccataat atataaatgt ggacttactt gagtaatacc tgaaacccag
      961 tccccttttt tgattgctgc agacatggat cgatcaaagc tagtaatttg atacgttgat
     1021 ccaatgttgg aagtagtatt tctttgttgg tcataatcaa ttagcaacac gtctttttca
     1081 aaaagttgac tagaaacaat cgctaataaa tcattgtctg tagtcttacc aatgccgcct
     1141 tttctacctg taagcattac aacttttcct agtttatctg atgttttaaa tgcagtccgt
     1201 aagttttcca tagttccctc caatttccta tactaaatat atttagtata cttcatatac
     1261 ttaatatatt cgatatatct aatatactcc aaattgataa gaattcagac tttataagga
     1321 atatagtgga tatactaaat atatttgcta tattcagtat atcagatata ttcaaaatgt
     1381 caaaatatat ttgtgcgttt tgcgttgaca atttagagtg aaaagaggta aacttaattc
     1441 tatagaaacc acagataatt cactatttac aaagtagcaa aagcagtggt actctttaaa
     1501 cagaaaaatt acataaaaaa aagagacgga aataattgat tttagcagaa tcaattattt
     1561 ctgaatcaca tggttaggac atgtgttcac gctcgtctca agaatatgta agtagctacc
     1621 aactacttat attaaaagtt gatcggatac cttttccgat tgcctttatt ctaccaaaaa
     1681 aaggcgagca actcaacaga agaacgttta tttagtgtac ttaaattaaa tattcttaaa
     1741 cactgaagag agaacacata ctatggctga gattaaagct aaactgtgtt ctctttttgt
     1801 ttgagaaagg acgttacaat cgatgttgag cgaaatttat tcagctattc ttaaaaacgg
     1861 cttacgtaca tacaaatatc gaaacagcac catgaaacct gcaggtcata ttgattccag
     1921 taggagtggt gctatctttg cgtaccgtag caagcaatta atgaatgttg ggcgtggtat
     1981 ggtcattact tcggaagaag ctattttaga gaacgaaaag aaactaacgc attggacacc
     2041 taatacctat cgctttggta cttatgcgga tgattatcga acagtggtta aaggtcattc
     2101 tgagaaaaac ttaagtcaaa tcaacacatt tgtagttgat atcgattcaa aagagaacca
     2161 tcaaggcgaa attattttag cctgtttaga tcaagtgggc tacatgccaa cgctaatttt
     2221 agaatcagat catggttatc aagtttattt tgtactaaag acaccaggct atgtcacaaa
     2281 gaaaagcaat ttcaaggtga tcgatgtagc aaaaaaaatt tcaaaaacaa ttcgtctaca
     2341 gttggctgaa aaattgtccg gagtggatct aggatgtaac cacttcggaa ttgctcgatt
     2401 cccacaagaa aataatattg tttttaaaga aatgaactat cagtattctt ttgctgactg
     2461 gattcaatgg tcgatgaagc ttgaatcaga tcaaaaaaca aaagaagatc gagaacgaaa
     2521 actaattgtc tttcctgaga aaaaagaaat tcgtcaagta gatgaaccgt ggttcgatat
     2581 gttacttcat aaagcaaaca tcgtcggcgg agaaggacgt cttggtcgaa ataatgtgat
     2641 tttcacgctt tctttagctt attactcttc aggttatgca ttggatactt gcgaatacaa
     2701 tatgttcaca ttcaacgaac ggttacagga accgctatct gaagcagaat tagtaaagat
     2761 tgtacgtagt gcttattctg gtaattatca agcagcaagc agagaattca ttattgaatt
     2821 gtgccagcaa tgggtttcac caaccttaaa aaataatgat ctgttcattc aacgacgtgg
     2881 ctggtggaaa tttaaaaagc cacgagatca aagaaaatat agtcataaaa atgaatggaa
     2941 agaagatcta ttggcttatc taagcgaaaa aagtcttgct agtgagcctt atttgaacct
     3001 gtcaaaaaaa gaattggctt cacaactaac gatgcccaag cgaacacttg ataagatctt
     3061 aaatgagtta cgagaggaga ataaagtctt ctacaaggta acacgaggaa gaaatggcgg
     3121 aatgatcatt gcttcagtca aaacgttgtt cgcgagggtt attcaactaa gtaaagaaaa
     3181 gagactagat tattcaacaa ggatcacaga ggtatttgga ctcaacgaga gcttagtaga
     3241 agagacattt agacaattat caaccccctt gaaccctctt aaagaagtga atctttttga
     3301 agtagataca ggttgaaaaa taaaaggtgc aaattgccct atacaatata tctatgatac
     3361 ttctgtaggt gttgctccct ttgcctagct acgtgtaggt ggtatactta taactgcgta
     3421 tgcagtctta tgtatcatgg aggaagttgt atgtcgttaa aaaattatgt ctattcacat
     3481 cctgttaatg tgtttatcat tggtcgtttg ggattatctg tggaagtgtt ctgtgagctt
     3541 tatggtttta agcaggggac tttgtcttct tgggttacca gggaaaaaac agttgcttct
     3601 ttgccgattg agtttcttca tgggttgtcc ttagcttctg gggagactat ggatcaggta
     3661 tatgattgtt tgtgtgtgtt agagcaggag tatattgagt ataaaatagc taacgagtta
     3721 agaaaaagga agaagtatat ccagtagtag tttttattta cttaaaaaat tggttcattg
     3781 agcaacctat cgctgctaaa gtagcaaagg aaaacgttaa aggaaagaat ttagggaaga
     3841 aatcgaaatg aaatcggacg actggattga acacacctgg cttcctctga aaagaaatag
     3901 caaacgaaag aaattttggg tattgcaatt atggccatta ggaaaacttt gtagttacgg
     3961 ggcacgactt attcttgatt taaccgttta ctcgattgga gtatttggaa cggcttatta
     4021 cttgataaat gatcccaata ggattgcagc atcagacatt tttacagtgc taatgacgtt
     4081 gatttttgtc atgctattgt ctatttttac tcaattagtc atgcaattta ccaggcaaaa
     4141 attaacagca ggaaaacttt tcaaaagcct attcgattta attgttgtaa atgcaattct
     4201 agcgacttta ataaaacaaa ttgaacaatc tggaagaaca acaaaaggag tgaaggattt
     4261 tttattgacg tttattcact ttgtaaatgg acacatgtat ttgtttgaag tgggtattat
     4321 tctctttttt gtcgggtatg tatccacgaa acttattaaa cgttcaattg aaaaagaaat
     4381 tgaaattgac ctgattccga cacttcttgg ttatgagtac aatgttgatt ttggaagata
     4441 tgacgaattg atcaatacgg atatcgttca cacgagtatc catgaatcta ctgttaaggg
     4501 catcagatct attttaaaaa atggccagga aatgaaagca atagatcgaa gaaaagagat
     4561 ctacaatttt tatgaagtcg aagcaagcct acgatatggc atcggaaaat tgtggttaga
     4621 tggaagtaat attcgtcaaa acaaaccata cattttttgg aacagtagtg atttagaact
     4681 tccaatgaaa gataaataat tatggagaat cgtttgaaaa tgattcctct atacaggatt
     4741 gtgcttatag ttggcatgga gttaaaaacc ttaaagaagg agcagttatt caaagtgaaa
     4801 gaacgtcata atcagttact atgtagtaaa acgggtacga tgaacattga ataccgaaca
     4861 gaatatttag aattaacgaa tcaaactgat tttttgttga ttcgaaagca acaagagcaa
     4921 gaaaaaaatt aatggctact tatagccaat tgattgatga atgggaacaa ttcaaggaat
     4981 ctaaaattcc agaacgtctc taaaagcctt tatttgtaaa gagcataagg aaacgtggga
     5041 acacttttta aaaaaagtgt tccaataggc tgtgcctatt acgtttctca aaaataaatg
     5101 tgggaacaaa atatttaaaa ataggatagt gaaaggatag tgaaggatag taaaaggagg
     5161 gtagtaggat agcaaaaagg ataggctcct tttcgctatc ctttttaaat tggaggaatt
     5221 aatatggaag taagaataag aaatgtggat gagaaaatcg taagagaaat tgatcgaaga
     5281 tgtgac
//
LOCUS       DAYYBG010000132         5191 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000132 DAYYBG010000000
VERSION     DAYYBG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.136"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACHAUN_002659"
     CDS             137..748
                     /locus_tag="ACHAUN_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8461299.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACHAUN_002660"
     CDS             821..1750
                     /locus_tag="ACHAUN_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFM8461300.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACHAUN_002661"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACHAUN_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACHAUN_002662"
     CDS             complement(2693..3895)
                     /locus_tag="ACHAUN_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8461301.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACHAUN_002663"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACHAUN_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5191)
                     /locus_tag="ACHAUN_002664"
     CDS             complement(4706..>5191)
                     /locus_tag="ACHAUN_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8461302.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1641 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta a
//
LOCUS       DAYYBG010000133         4998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000133 DAYYBG010000000
VERSION     DAYYBG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.137"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..1791)
                     /locus_tag="ACHAUN_002665"
     CDS             complement(25..1791)
                     /locus_tag="ACHAUN_002665"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8461303.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1751..3505)
                     /locus_tag="ACHAUN_002666"
     CDS             complement(1751..3505)
                     /locus_tag="ACHAUN_002666"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8461304.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3615..4184)
                     /locus_tag="ACHAUN_002667"
     CDS             complement(3615..4184)
                     /locus_tag="ACHAUN_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8461305.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4202..>4998)
                     /locus_tag="ACHAUN_002668"
     CDS             complement(4202..>4998)
                     /locus_tag="ACHAUN_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8461306.1"
                     /translation="IDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIE
                     KVSADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGK
                     IIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQ
                     LPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVI
                     ATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1655 a          943 c          674 g         1726 t
ORIGIN      
        1 taggttaatt taaagcctat ttcatcatcg tgattgagct tctttttcat agacgaattt
       61 ctcagatagt ttagttttct ttatgagatt tttgtatatt tctgaccttc gatacaactc
      121 actatgagta ccttgatcaa ctatttttcc ttgatccatc actacaattt tatttacctt
      181 ttcaatagac ttcaagcgat gcgatataat tagtactgtc ttatcttgta ttaaacgatt
      241 taaactttcc tgaatacgtt tttcattttc cgcatctaaa ctggcagtaa tttcatctaa
      301 aattaaaatt ggcgcatttt tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc
      361 tccagaaagt gaacttccgt tttctccgac caatgtatga taaccgtcag gtagtttttc
      421 aataaagtct tcgcagttag ctaaacgagc tgcttttttc acttcctcat ctgacgcttg
      481 actatttcca atacgtatat tttccaaaat acttgtatta aacaaagtta cctcttgaaa
      541 aacaatagcg attttgctaa acaaagaagc tggagataca cgtttaatat catacccatc
      601 aattaaaata cagccttcat cataatcata cagtctagac actaatttca agatactagt
      661 tttcccggaa ccactagcac ctactaacgc agtcacttct ccttgtttag ccgtaaaaga
      721 gatatgatct aaaattgggg tattgttatc atatgagaaa gagacatctc gcaactcaat
      781 gtcaaaagat ttcagaggac tatctgatcc ctcttggata ctggtttcct tcatagctct
      841 aattcgctgt attttaggag ctaaataaaa tatttcaagc actgcttctt tcatagaatc
      901 gaaggaatcc tttattttta ttgcggctag taagtaacca acgacgtaga gtatagtcac
      961 ttcacccgtt aacagtaagt gaacacctac tagaatgact actgctaaac taacatatga
     1021 gaaaatagag gataaggcca tgactgaaaa agtacttaat tctaccttta aatgaatcct
     1081 ttcactctct tccatttttt tgaaaagtct gtcttgaact tttttagata gattaaagct
     1141 attaatctct tgctgcaatt ctatagtttc ttgaaattct tccgagtttt ctctcaaagt
     1201 atcgtaatat ttagtattgg ctttcgtttg ggatttcttt gataacaaga ttaagacaaa
     1261 actaaataac gttggcaaaa taacagctaa tcccattttg acattaccaa ccaaaagcat
     1321 cactgaaata aaagggaaaa acagagccat accgccggct ttaggtattg catgactcat
     1381 cgcatgctca ataccttcaa cgtcagacat gatagtttga gataaatctg ataaattatg
     1441 ttttgaaaaa taggataatg gtaaatttga caaatcgtct gcaatttgca ttcttaaatg
     1501 cgcactttct ttataagtac tattatataa gttttcatac tccctatcta acaaccaata
     1561 catgaaaagc aaagtaacga acgatactgt aaaataaagc caaggcggct ttacattttc
     1621 taaaactaat tcgtctatca gcatcattat aatgaatgct ggtaccatat taatgcagta
     1681 tacaaaaaag ctagaaatag atgctttaac caaatcttct gcgccctgat tagttaaagc
     1741 gaatttgttt ttataaaaac ttttcattgt caagcctcca atcatttgca ttctcataat
     1801 tagtgaccaa ttgtttatac acttgatgtt tagccattaa ttcattatga tttccacgtt
     1861 ctactatttt cccttctgac aagacaagaa tttcatctac agatttaatg ctggataatc
     1921 gatgagctat cataataact gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt
     1981 catactcatt atctggatca atagatgcac tggcttcatc cataataaca atctttgcat
     2041 cttttaaaat tgctctggca atagctatac gttgtttttc tcctcctgac agataaactc
     2101 ctttactacc aattaaggta tgctctcttt cgggaaattt ttctaaaatc gattcgcaac
     2161 cggctaatct tagcgcgtca aatactttac ttgctggcgc ttgaggattt gctatagaaa
     2221 cgttatcgta aatactctga ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt
     2281 taataattgt gtcttttgaa tagtgattta aaggcttacc accaatttta atctgtccct
     2341 tatccacatt gtagaaacct gaaattagtt tagctatcgt actctttccg cttcctgatt
     2401 ttcctactaa tgcataaatt cttttttgtt ctaaaactaa gttgaaattt tctaaaacac
     2461 actcttcttt gtatgaaaag ctgacatttt caaatgcaat gtcacaattt ttaatctgtg
     2521 tctcgttccc atattttagt tggtcttgct tcatttctaa ataaagggag tctagtgttt
     2581 cgactgcata tcttgcttca aacaagtgca tacttacata cattattctc atgaaagaaa
     2641 caaaaataat tccacttaaa aaaaacaaca tcaacaagtc aacagctaat atcttgggag
     2701 tggtaagaga agttataaaa aaggttatag gaactatcaa aatgcaaact atgccaaaaa
     2761 atagccattg aaaaagaaca aaaggtgttt tcccactcaa agaatactta tatgcaaact
     2821 tagcataatc gttaattgct ttattcaagg ctttaaatga tgttacttta gcaccaaata
     2881 tctttataac ttgaattcct cggatatatt ctaccgtttc agagcttaac acatctaacg
     2941 attcttgata tttttgaata aaatttgtat tctctgtcat tttctttaac aagaatacac
     3001 caattactaa catggcgatt agaaccaatc caattttaaa attaatgaaa aagcctaagg
     3061 caactgataa aacaggtgtt actatggctt gagcactatc tggaatcaag tgagcaattg
     3121 ccatatgtgt tttagccgcg ttgtcatcaa ttgtctttcg aatatatccc gaagaattta
     3181 aatcaaaaaa acgaaaactt gcttctgtta aaccgctaat tccctttttt ctcaaattag
     3241 tctctaagcg aaacccaagg atatgagaaa ataagccaga aaaaatataa aataaagctc
     3301 ctaaagttaa ccaagcaaca gtttgaaccg cataactctt agctaaatat tcatctccta
     3361 caataattag agacttaaga aatttgtaaa tactgtaata tccatagact gttaatacga
     3421 cagaaaaatc agaaaataaa gtggctaacc atccgtagta tttcatttta gggacataac
     3481 taaataattt tttgtatact ttcatttttt cctcccccgc attcaatttc ttctcttatt
     3541 cgtttacaac tgttaatgct ttccgtaata gcgtaaatta gagatgtaca aacagataga
     3601 aggtaaacta cgttctattt tttgtatttt tttaagactt ttcttccaat caatacacca
     3661 atcatagata aacttgctac aactaacact gaaatgattc cccatatacc taccgcatag
     3721 tggatcattg catccattgt tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa
     3781 cgatctgtaa agaaccatat aggaatccac tgtccggcag cactgcctaa ttgcattaga
     3841 gcccaagacg caacttgagg aattgctcga tcatatccaa atacagatag aataatatca
     3901 gctaagatcg ccatactaat cgaactgata aaccaaggta agtacgcccc gcccattaac
     3961 gtaaaaaata acaataaaac aattgtgtaa atagtaaaaa ttccttttct agggtagtta
     4021 tagctaatat aaacaaatat gattccagat aataatccaa aaatccctgg ggatatgctg
     4081 tgaccaaacg ccccaccaat actactaacc attattgaaa aaaagtaaat tactagggaa
     4141 ataactgtag ctaaaccaat aaaaatataa tcttttgttt tcatatatat tctcctcaat
     4201 tctatttcgt tatgtcatta aaaatctgtt ctatcaaagt tgaatcttct actctaagtt
     4261 ttatatcttt ttttattcca ccatttttta aataaataat tctattgcat gttttataaa
     4321 gaaattccgg atcatgagtg gctataacta ctgcaccctt ttcttttaag tctttaataa
     4381 ggtcagcaat cctttccata ttaatataat ccaacccact tgtaggttca tcaaatagaa
     4441 ataaattagt ttttgacaat gaagctatag aaaccaataa tctttgtttt tctccaccgg
     4501 ataaatcaaa cggtaattga tctttcattt tataaagttc catttttttt aaaatttttt
     4561 cttgctgatc tttactaact atttcattcc ccagggctaa ctcgctcctc actgattctg
     4621 tgaaaaattg gtaatccatt tcttgcatta ctaaaaacgg cgatgacaaa ttttttttat
     4681 tttcgattat ctttcctttg gtagggcgaa taatcttcat gagtattctt agtaaggttg
     4741 ttttccccac accgtttttc cctaccaaac caataacatc tccaactttg acatcaaaat
     4801 ttatgtcttt tagaatattt tcataagtaa tttcttcaac ttttaataca tctgcactta
     4861 ccttttcgat atagggaact tcaatcgaac tattaaaaat gtcaaatttc cttaatgcgt
     4921 tattgttttc agtttttaac aactgaatag ttgtagtttt tacagttcta tttttaacaa
     4981 atagaacttg atcaatta
//
LOCUS       DAYYBG010000134         4977 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000134 DAYYBG010000000
VERSION     DAYYBG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.138"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHAUN_002669"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHAUN_002669"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8461307.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHAUN_002670"
     CDS             complement(1297..2133)
                     /locus_tag="ACHAUN_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461308.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHAUN_002671"
     CDS             complement(2317..3534)
                     /locus_tag="ACHAUN_002671"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8461309.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHAUN_002672"
     CDS             complement(3683..3844)
                     /locus_tag="ACHAUN_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461310.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHAUN_002673"
     tRNA            3917..4000
                     /locus_tag="ACHAUN_002673"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4977)
                     /locus_tag="ACHAUN_002674"
                     /pseudo
     CDS             complement(4240..4977)
                     /locus_tag="ACHAUN_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1526 a         1027 c          798 g         1626 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttatt
//
LOCUS       DAYYBG010000135         4971 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000135 DAYYBG010000000
VERSION     DAYYBG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.139"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(489..863)
                     /locus_tag="ACHAUN_002675"
     CDS             complement(489..863)
                     /locus_tag="ACHAUN_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8461311.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(886..2286)
                     /locus_tag="ACHAUN_002676"
     CDS             complement(886..2286)
                     /locus_tag="ACHAUN_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFM8461312.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2290..2700)
                     /locus_tag="ACHAUN_002677"
     CDS             complement(2290..2700)
                     /locus_tag="ACHAUN_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8461313.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2718..3209)
                     /locus_tag="ACHAUN_002678"
     CDS             complement(2718..3209)
                     /locus_tag="ACHAUN_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8461314.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3219..4010)
                     /locus_tag="ACHAUN_002679"
     CDS             complement(3219..4010)
                     /locus_tag="ACHAUN_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8461315.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(4003..4776)
                     /locus_tag="ACHAUN_002680"
     CDS             complement(4003..4776)
                     /locus_tag="ACHAUN_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8461316.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1575 a         1012 c          667 g         1717 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agtaaattct cattaaacta
      421 ttgaaatgct gataccaaaa aatgaagtac ttttcgttca taccaactta gagcacttcg
      481 ttatctattt atttcttcat ttggcttatc tctataatct catgatttgg tccgtagaaa
      541 ttaaagtaac ggattccatt ttcccagaac ggtaattcca taatattttt atctaataat
      601 tcatatccta aatcttgaat tgaattgaat acctcatcta tgtcatccgt atctaatgct
      661 agatgatcaa tggctcctct acactcagta gttttatctt cccatatttc tattatcgtt
      721 ccattcaact cgaaaaagcc gactttactt cccacattca tttcttcatt aatcagacta
      781 aaccctaagg attcataaaa gatttttgaa gctccaaaat cttttgttgg aattccaata
      841 tgttgtaaac catttaattt cacttacatc atcccttcaa ttgttttaaa atccacattc
      901 ataatttatt gaatagtcta gtttatctac cttgattgct ccctccgtaa cagtcatact
      961 taatgctgtt ccgactttat catacattct agtacttaaa gcaacattat caatatataa
     1021 aacagctatc gtatcatcaa caattaactt tagttcatat tctttgccta tttccaaaaa
     1081 tagtggtcgt tgcagcccct tatcaaacat ttgaggccaa ggatagttcg gagtttttcc
     1141 gaaagtaagc tgattattaa tcaatgaaat acgaaattcg tatccttctt gagtatcact
     1201 atctacaaat aattttaaag caaactcctt tgttaacgat gtaatagtca aagttgtttc
     1261 aaatagaaaa taatcttcga ttttaggaac aattactctt tctttttttc caaattcttt
     1321 ctctaactta aaacttgaaa atggagtgat ttctttaaaa ttctcaatta ttttttcagg
     1381 cagtttaaca ccaagagttt tgtctgtctt ttgaataatt tgatgtggaa caagtgcacc
     1441 gccccaatcc cagttatttt gatcgttctc cccttctttt gttgccaccc agccagttag
     1501 aaaacggcca gtttcattag ttccgactgt tcttgctgca taataggctt ttccgtcgaa
     1561 agcttcgtct actggtgatt tccaatctga aaataatcct gctccgactc gatatttagt
     1621 ctttttatct tcacaatact ctgaaaaaag taaataccac gttccatcaa tttcaaatat
     1681 atcaggcatt tcaatcatgt taaattcatt attagcccag tagtctcctt tgaaattcca
     1741 atcttttaaa tcttcggatt caaaatagac caatctcccc gtcggctttt tcttacttgc
     1801 tggttttcta gctcctagta tcaaaacgta ttttccatga tcttgatcaa aaattgcaaa
     1861 aggatcgcgc caatcattct tatcatatcc ttcttgagga acaagttttg ctgcttcgcc
     1921 atcctttttc caatgaatta aatctttaga acttgcaatc attaacactt ctgaagtttt
     1981 tccatccaac ttagctttgc gattatgtcc agtatacaaa gctaaatact cattgattcc
     2041 ttggacaata cttccagcgt aaatgtattg atcaatgtca tcgttgccac cacgatgaat
     2101 tacttccccg tattcttcaa atttcacaaa atcagtggtt gtaactagtg accacccaaa
     2161 aggatcagtt aaaggtttag gatttctcgt atctcgttga tgaaatagat aaaaagttcc
     2221 atctttataa aaaggcatgc aatccccaaa ccaaaaatct ttaggcttgt aaaaaatttt
     2281 ttgcatttat cactcatcct cttcgttagt atgtctttgc atatctgcaa gacacgcaat
     2341 tgtattcata tttcttagat tttcgatgat atcatcgctt ttttttcctt gtaatctcaa
     2401 attgacagcc tcaatcaaaa ttcctaaatt catgccacca agaagttgaa acccattttg
     2461 aaatacttca cttcttttag caaattcgtt gaaaggtgtt ccaccaatca agtcagcaaa
     2521 taccataact tcttgatttt ttacttgaga aactattttt tcaatgtttt cacggaattc
     2581 ttcaatgcct gcatctccaa gtgttaccac atgacaattt aaattatcgc caaaaaacat
     2641 ttttaatgta tctacaaatc caatggcaat atttccatga gatgtaatta taaactccat
     2701 ccactcatcc tctgttctta ataaataaaa tcctttaaaa attcttttga atctctagga
     2761 gtttgtatca ctgtcacttc aactccatca ctaattagtt ctttaaaagc ctctctctct
     2821 tcttcagtaa tatttatcgt cggacggatg cgttctgttc cattccttct tgataaattt
     2881 cctacgttta cacttgttat tggtaaaccg ttatcaatca aaaatttaac atcgattgga
     2941 gaaactgcaa caataagaac tttttgtcct tcatatttac cagaaagaat atttttaact
     3001 gctacttccc ttgtaattat tgaagttcta acattaggtg gtgcagcgag gcgaactaca
     3061 gcttttttta cgtcatcatt ggctacttca ttattaataa ccataattct gttagcactc
     3121 aattgatttg tccattgtgt tgctacttgt ccatgaatca ttcgatcatc aatacgaaca
     3181 tgtataatac ctttcatttt ttcatccata ttcaacacct acttcaaaat atttaacgct
     3241 cttaacgcaa tagaaactac taagattaat agaattacat aaactggttt aacctttcgt
     3301 cctaacaaat aatacacaat tgctactaca catgcaggta ataaaccagg cataacttga
     3361 tcaaagattt catttccggt cattgttaag tcgccttgtt taaattcaaa gccaacattc
     3421 acttttacaa ctgtggcaat gagtgcaccg accaccatta accctaaaat tgatactgca
     3481 tcagtaatag actttaattt tgtttcaatt tgagttagta ggctcgcacc cgattctttc
     3541 ccagcaaggt ataaaggata agcagtaatc cttaataaaa ttgatgccag tatccacaaa
     3601 ataattccaa caaaactacc ttccaatgcc atattagctg caattgctcc aaaaatagtc
     3661 catggaataa ctacaaatac actatctcca attcccgcta aaggacccat tagacctgtt
     3721 ttcagagcac ttactgcttc caaagactta tttttttctt ctttttcaat tccaacattc
     3781 ataccaataa tgtaaggcgc tgtatacggc gtagtgttaa aaaattgtag gtgagctaaa
     3841 acggattgtt tcattccttc aggatcatct ttatatgtgt ccttgataaa aggaagcata
     3901 gaatacgcat aacccaaccc ttccattttt tcataattta atgaagttgc accattgaga
     3961 atccaccgga aaattacttg tttgtcgatt tttgtcagtt tcttattcat cttcgtcaaa
     4021 acctcctgtt gcagctggtg ctacttgagt attatttgat ctatttttat atacgattag
     4081 tgcgattgca gcgccaagta acgaaatccc taaaattggt atttggaaat aaactgccaa
     4141 aacgaaacca actaacaaat aggcaaagta atcttttgta ggtaaatatt taagtagcat
     4201 tcctatccca acagcaggta atagtccccc tgctaccttt aaaccaccgg taacccattc
     4261 aggaatataa ttgattgcac ttcccactaa cgttgggcct aatatcacag ttaacaaaac
     4321 aggaattcca gatacaaagc ttgtattaat tgatgccaaa tattgcatcc actctaattt
     4381 ttgataatct tctttttgtg cgaatttttc tgccttttgt tgaaaaaaaa tgtttgttga
     4441 ccactttaga acatctagtt gtacaattaa cattgctacc ggtattgcta aagttattcc
     4501 tatagaagca tcctggttag ttgagattgc taaataagta ccgatcaatg ctgctgtttg
     4561 ataatccggt actgatgcac caccaaaact agtaatacct agtgtcatta attgcaaagt
     4621 cccaccgata taaagtccag tttgaatatc tcccataata agtccggtca agaaacctgc
     4681 aacgacaggt tgggaggttc cgattttggg cccttcctta tcccagttaa tgaaaaatcc
     4741 atacaacaca actaaaaatg tttgccaaaa cgccattttg ttctctcctt tcttcttata
     4801 cttttatata agcaattact gtgccaacta acattatcca ataaagcggg aaaatacagt
     4861 aaatattaat taaatgagcg tctaaatata ctttggacac cctttcattt ttttagacgc
     4921 tagtatgcta tttagaaaat agtctgctca aaaattgtat agattaactc a
//
LOCUS       DAYYBG010000136         4931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000136 DAYYBG010000000
VERSION     DAYYBG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.140"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..970
                     /locus_tag="ACHAUN_002681"
     CDS             83..970
                     /locus_tag="ACHAUN_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8461317.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="ACHAUN_002682"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="ACHAUN_002682"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8461318.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="ACHAUN_002683"
     CDS             2272..2718
                     /locus_tag="ACHAUN_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8461319.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="ACHAUN_002684"
     CDS             2991..4343
                     /locus_tag="ACHAUN_002684"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8461320.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="ACHAUN_002685"
     CDS             4514..4801
                     /locus_tag="ACHAUN_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8461321.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1395 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt t
//
LOCUS       DAYYBG010000137         4924 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000137 DAYYBG010000000
VERSION     DAYYBG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.141"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            198..998
                     /locus_tag="ACHAUN_002686"
     CDS             198..998
                     /locus_tag="ACHAUN_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8461322.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1017..2885
                     /locus_tag="ACHAUN_002687"
     CDS             1017..2885
                     /locus_tag="ACHAUN_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8461323.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            2878..3369
                     /locus_tag="ACHAUN_002688"
     CDS             2878..3369
                     /locus_tag="ACHAUN_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8461324.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3357..3911
                     /locus_tag="ACHAUN_002689"
     CDS             3357..3911
                     /locus_tag="ACHAUN_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8461325.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            3929..4924
                     /locus_tag="ACHAUN_002690"
     CDS             3929..4924
                     /locus_tag="ACHAUN_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8461326.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1719 a          769 c         1033 g         1403 t
ORIGIN      
        1 aacaattata tatcaaaaag agaaacattg cttatataac gctcaggaat tttagcaatc
       61 tatgcaaaaa gatgaaaatt ttttttgctc ttccattagt acacattttt tagctgaagc
      121 tgtaaatttc atgtcttaat tttttttagt gagcttttat actggatttg taagaaaaaa
      181 gaaggagagg atcacatatg tcggattggt tggatattgc aggaaaagta gtaattgtaa
      241 ctggaggttc ctcaggaatt ggaagaagta tcgtcgaaaa tttgttgaag cagaatgcac
      301 aagtagcgaa ttttgatgta actgaatgcc gtatacagca tgaaaaccta ctatctttaa
      361 aagtcgatgt gtcttctaaa acggacatag aagaagggat atataaagta atgaaacatt
      421 tcaagacgat cgatggtcta gtcaataatg caggaataaa tattccttct cttctaatag
      481 atagaaatca tccaaaaagc aagtatgaat tatcagaaca agtttttgat aagatgatcg
      541 ctgtcaatca aaaaagtgta tatttgatga gccaagcagt tggaagaatc ttggtgcaaa
      601 agggatcagg agtgatcgtg aatctttctt ctgaatcagg attggaagga tcagaaggac
      661 agagctgtta tgctgctaca aaagcagcga tgaacagttt tactcgttct tgggcaaaag
      721 agttaggaaa gaggaatgtt cgtgtggtag gtgttgcacc agggatactg gaagaaaccg
      781 gtttgagaac acaagaatat gaagaagctc tttcttatac gagaggtata agcgtcgaac
      841 agctgagaaa cggttacagt cgaacaagta cgatccctct aggaaggagt ggtaagctgc
      901 aagaagtggc ggatctagtc tgctattatc tttcagaaag atcaagttat atcactggag
      961 tcacgactaa tatttcagga ggtaaaacaa gaggataaag gatagggggg acgcacgtga
     1021 cgctagtaaa cagatggtac caattattga aattactagt cactcacaag aaagtttcaa
     1081 tccaggaagt gcaaaaagaa atcaaaacca gtaatcaaac cataaaaaaa gatattgggg
     1141 agttgaatga agaaattgcc ggtattgcaa aaatcatcca aaaaaacaaa cattttgaac
     1201 ttgaaattta tgattttgat ttttttgatg agatcatgca aggaaaattg aaaacagcca
     1261 gtgatttcaa ttcggcatcc aaaagaatcg cttatttact aaataggctg atcgaaacag
     1321 aggatttctt acgtatggat gatttgtctg aagaagtcgg tgtcagcaga ggaacagtgg
     1381 tcaaggatct aaagacatta aaagagatga tcaatgattt tgatgtaaag attacaggca
     1441 caccaaataa aggactaaga ataataggag atgagctaga attacgacta cttttatttt
     1501 attttgtttc tccctacttt tcagagacga aaacagaaca gtttctagaa gaaaaacaag
     1561 caatcaaaca ttttcagaag aagacgaatg ctagtaaaca agaaatcacc cttttaacga
     1621 aagttatcgg tatttctttg aatagaatct ctagtaacta tctgttgaag aagcccatca
     1681 atcgttacag tgggtgtttt gaatatatcc aagaattcaa tgaactgatc aatcagttgg
     1741 aagaaatcta tgaactaact ttgagccaat atgaaaaaga ttttatcagt tttcctttga
     1801 acatcatcaa cacaagtata acgatgataa ggaaagctaa ctctgatttg cgcttatatt
     1861 tcgaccccat gatgatgcgt attcgacgat tagatctcat tgatctggat gagtccttct
     1921 tatatcaaga gatgaaacag cacctctctt tattgataaa tcgggtaatt tttcgttatc
     1981 gattgactga tttattttat ggtgagatag aaaaaaagta tcctttttct taccaaatcg
     2041 ctagcgaatg catacaagca ttagaagcta tcttgcaaca taagatctct aaagtagaga
     2101 tcagctacct aacgttgtat ttggagttag catttcgata tcaaaaagag catttttcct
     2161 ctaaaaaagt agccattgtt catacgacag gaaaaggaac agcattaatg atccaaagac
     2221 aaatcaaaag gatattgggg agggatatac aaataacaca tgtttcagaa gaacaatatg
     2281 aaaaagtaga tctaaacaaa tatttcgctg tttttacaac gattccgtta aaaaatgtaa
     2341 atgaagagac accggtgatc catttgacta atctttttga tgactcttgg ttaagggcac
     2401 aatggcaaaa agcaaaaaaa aagcgattag ccgggtttca ttctactgtt tttttctatc
     2461 aaatatggaa agaaaaaaag acgtatcaag aatatttaaa caaattagtc gctcctttga
     2521 tcagaggcta ttatgtggat gaagaatttg aaaaaaggat cttgaaaaga gaatatcagc
     2581 aaagcacatt tgttaacaaa gaatttgctt ttcctcacac aaccaaccag ctatctcaac
     2641 acattgtttt atcagtaggg ctaataactg atcaaggttt agcagaaaca ggaggaaatg
     2701 gagttatttt tttatttgcc atacctgaag aaatgacggc tgaagttgag aatgaattat
     2761 ttgagctata cgatatccta tttgaactca taaaaatggg aatagatgag tttattgaat
     2821 tagcaccgac acaagaatca tttttaaaac taatcaaaag taaaggagag agacttcatg
     2881 aatgatatgt atctattcat agttttcgca gcttcagctt atgtgctgca agcagttatc
     2941 agttggaagc aactaaagca tttcaatcaa acatatagcg aactgcgaag aaaaggaaaa
     3001 gtagcgattg gtaaacgacc tgggaaaatc cgttcaggca cgattgttat gctagctata
     3061 gacgatcatg atcaagtttt agaagtcaga aaaatgcaag gggttacatc tttggcaaaa
     3121 tttcattcat tacctgtcta tgcgggtgaa gatatccatt acatcgatcg ttaccatccc
     3181 tcagttcgag cggaagatcg tttgacacaa gcagctttag aaaatgccag agaagtatac
     3241 ttacgaatag aggcaggaaa tgaaaaaagt gaacaacctt cattgccttt tattgctatg
     3301 ctcaataatt ttcggatatg gaaaagtacg atcaaagaaa aaaggagtga caaacgatgg
     3361 atatcataac aaaatttgct gagggattta tgaggctgtt ccaaacaggt gcagaaacat
     3421 tcataagctg gatgacagga atcgtaccag ttgtcttgat gctgatggtt gcaatgaata
     3481 cgctgattgc ttttttaggt gaagaccgag taacgaaagt agccaaatta tcagcgaaaa
     3541 atccgcttac tcgttacctt gtgttaccgt tcttatctgc ttttatgttg ggaaatccta
     3601 tgtcatttac aatggctcgt ttccttccag aatactacaa accaagctat tatgctgctc
     3661 aagctcaatt ttgtcatacg agcaatggtg tattccccca tattaatcct ggagaattgt
     3721 ttgtatggat ggggattgct caagggatcg aaaccttagg gctaaattcg atggagctgg
     3781 ctatccgata cttgcttgtg ggactgctga tgaactttat tggtggttgg ataactgatt
     3841 ttactactgg atttgtatgc agacagcaag gaatcgttct tagcaaaaaa gtagagcttt
     3901 cagttgatta gagaaaggag agttttaaat gtatggtgat attaagatcg taagaggaaa
     3961 cggcgggtat ggaggtcctt tgattattag gccgacaaaa gataaacaca agttcatcta
     4021 tgtcacaggt ggaggtgaga ggccaatgat cgtagataaa atagaagaac tgacaggtat
     4081 ggagtctatc aatgggttca aaacatcaat acctgatagt gaaatcgcgc ttgcaatcgt
     4141 tgactgtgga gggactctta gatgtggaat ttatcctaaa aaagggatac caaccatcaa
     4201 tatcgtttcc acaggaaaaa gtggcccatt agcacaatat atcactgaag atatttatgt
     4261 ttcagcagta gggttagaac aaatacagcc actaatggaa actgatgata acaaagaaac
     4321 agtaaatact gcatctccag ataaaaagta caaatatgat acttctaaaa agataaccga
     4381 gcaaaaagca gagcagcaaa actggatagc aaaaattgga atgggcgcgg gaaaagtagt
     4441 tgctacattc aatcaatcag caagagaagc agtagatacg atgatccgga cagtgatccc
     4501 ttttatggct ttcgtttccc ttttgattgg tgtgatacaa ggttctggga tcggtaatgt
     4561 atttgctaac attatggcac ctttagcagg aaatgcagtt ggattaacaa tcattggctt
     4621 tatttgttca ctgccatttt tatctccttt gcttggtcca ggggcagtta ttagccaagt
     4681 gattggaaca ttgatcggtg tagagattgg gaaaggaaat attcctcccc aattagcatt
     4741 accggcacta tttgctatca atactcaaaa tgcatgtgat tttattccag tcggtctagg
     4801 tttggaagaa gcaaaagctg agacagtaga agtaggtgta ccatcagttc tttattcaag
     4861 atttcttaac ggcatcccac gagttatcat tgcttgggta gcaagttttg gattatatag
     4921 ctga
//
LOCUS       DAYYBG010000138         4892 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000138 DAYYBG010000000
VERSION     DAYYBG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.142"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACHAUN_002691"
     CDS             complement(287..649)
                     /locus_tag="ACHAUN_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461327.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACHAUN_002692"
     CDS             complement(723..1103)
                     /locus_tag="ACHAUN_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8461328.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACHAUN_002693"
     CDS             1185..2360
                     /locus_tag="ACHAUN_002693"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8461329.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACHAUN_002694"
     CDS             complement(2437..3021)
                     /locus_tag="ACHAUN_002694"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8461330.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACHAUN_002695"
     CDS             3222..4220
                     /locus_tag="ACHAUN_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8461331.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACHAUN_002696"
     CDS             4332..4841
                     /locus_tag="ACHAUN_002696"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8461332.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1553 a          914 c          851 g         1574 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt tt
//
LOCUS       DAYYBG010000139         4757 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000139 DAYYBG010000000
VERSION     DAYYBG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.144"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACHAUN_002697"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACHAUN_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM8461333.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACHAUN_002698"
     CDS             724..1878
                     /locus_tag="ACHAUN_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM8461334.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACHAUN_002699"
     CDS             2093..3061
                     /locus_tag="ACHAUN_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM8461335.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACHAUN_002700"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACHAUN_002700"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM8461336.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACHAUN_002701"
     CDS             4091..4699
                     /locus_tag="ACHAUN_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFM8461337.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DAYYBG010000140         4700 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000140 DAYYBG010000000
VERSION     DAYYBG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.145"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACHAUN_002702"
     CDS             complement(136..1098)
                     /locus_tag="ACHAUN_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8461338.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACHAUN_002703"
     CDS             complement(1100..2539)
                     /locus_tag="ACHAUN_002703"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8461339.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACHAUN_002704"
     CDS             2724..4670
                     /locus_tag="ACHAUN_002704"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8461340.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1487 a          807 c          776 g         1630 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt
//
LOCUS       DAYYBG010000141         4692 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000141 DAYYBG010000000
VERSION     DAYYBG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.146"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1642
                     /locus_tag="ACHAUN_002705"
     CDS             152..1642
                     /locus_tag="ACHAUN_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8461341.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            1990..2160
                     /locus_tag="ACHAUN_002706"
     CDS             1990..2160
                     /locus_tag="ACHAUN_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461342.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            2174..2791
                     /locus_tag="ACHAUN_002707"
     CDS             2174..2791
                     /locus_tag="ACHAUN_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8461343.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            2791..3729
                     /locus_tag="ACHAUN_002708"
                     /pseudo
     CDS             2791..3729
                     /locus_tag="ACHAUN_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4422..>4692)
                     /locus_tag="ACHAUN_002709"
     CDS             complement(4422..>4692)
                     /locus_tag="ACHAUN_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFM8461344.1"
                     /translation="EMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNSSFLLPIITIG
                     KFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT         1715 a          732 c          858 g         1387 t
ORIGIN      
        1 aataatttat tcgattagtg gaaaaaaatt gacttataaa ggaaaaaaat ctttttcaaa
       61 acatgcaata ttgaaacagt tgaatgaaaa agcaaaccaa gttaattaaa caacctattt
      121 tataggattt ataggaaagg agaacagctg aatgaatatc ccttttgttg tagaaactgt
      181 gcttcatgac ggcttgttaa agtacaaatt taaaaatagt aaaattcgct caatcactac
      241 caagccaggt aaaagcaaag gggctatttt tgcgtatcgc tcaaaatcaa gcatgattgg
      301 cggtcgtggt gttgttctga cttccgagga agcgattcaa gaaaatcaag atacatttac
      361 acattggaca cccaacgttt atcgttatgg aacgtatgca gacgaaaacc gttcatacac
      421 gaaaggacat tctgaaaaca atttaagaca aatcaatacc ttctttattg attttgatat
      481 tcacacggca aaagaaacta tttcagcaag cgatatttta acaaccgcta ttgatttagg
      541 ttttatgcct actatgatta tcaaatctga taaaggttat caagcatatt ttgttttaga
      601 aacgccagtc tatgtgactt caaaatcaga atttaaatct gtcaaagcag ccaaaataat
      661 ttcgcaaaat atccgagaat attttggaaa gtctttgcca gttgatctaa cgtgtaatca
      721 ttttggtatt gctcgcatac caagaacgga caatgtagaa ttttttgatc ctaattaccg
      781 ttattctttc aaagaatggc aagattggtc tttcaaacaa acagataata agggctttac
      841 tcgttcaagt ctaacggttt taagcggtac agaaggcaaa aaacaagtag atgaaccctg
      901 gtttaatctc ttattgcacg aaacgaaatt ttcaggagaa aagggtttaa tagggcgtaa
      961 taacgtcatg tttaccctct ctttagccta ctttagttca ggctattcaa tcgaaacgtg
     1021 cgaatataat atgtttgagt ttaataatcg attagatcaa cccttagaag aaaaagaagt
     1081 aatcaaaatt gttagaagtg cctattcaga aaactatcaa ggggctaata gggaatacat
     1141 taccattctt tgcaaagctt gggtatcaag tgatttaacc agtaaagatt tatttgtccg
     1201 tcaagggtgg tttaaattca agaaaaaaag aagcgaacgt caacgtgttc atttgtcaga
     1261 atggaaagaa gatttaatgg cttatattag cgaaaaaagc gatgtataca agccttattt
     1321 agtgacgacc aaaaaagaga ttagagaagt gctaggcatt cctgaacgga cattagataa
     1381 attgctgaag gtactgaagg cgaatcagga aattttcttt aagattaaac caggaagaaa
     1441 tggtggcatt caacttgcta gtgttaaatc attgttgcta tcgatcatta aagtaaaaaa
     1501 agaagaaaaa gaaagctata taaaggcgct gacaaattct tttgacttag agcatacatt
     1561 cattcaagag actttaaaca agctagcaga acgccctaaa acggacacac aactcgattt
     1621 gtttagctat gatacaggct gaaaataaaa cccgcactat gccattacat ttatatctat
     1681 gatacgtgtt tgttttttct ttgctgttta gcgaatgatt agcagaaata tacagagtaa
     1741 gattttaatt aattattagg gggagaagga gagagtagcc cgaaaacttt tagttggctt
     1801 ggactgaacg aagtgaggga aaggctacta aaacgtcgag gggcagtgag agcgaagcga
     1861 acacttgatt ttttaatttt ctatctttta taggtcatta gagtatacct atttgtccta
     1921 atatgatttt agcagtataa ttgacttggt gaataggtca tttaagttgg gcataatagg
     1981 aggagtaaaa tgaaaaaatt tatttatcga gttttagaaa atgacgaagt ggtggctatt
     2041 tttaatgagc aacaatatgc gcaagatttt atcgcttacg aaaagacaat ttctgataag
     2101 caatttgaaa ttgaaaaagt agatattgct gattggttat tgcaaccgag agaattttag
     2161 aggttggttg aaaatggcta aaattggtta tgcacgtgtc agtagcaaag aacagaactt
     2221 agatcggcaa ttacaagcgt tacagggcgt ttctaaggtc ttttcagaca aattaagcgg
     2281 tcaatcggtc gaacgcccac aattacaagc tatgcttaac tatattcgtg aaggggatat
     2341 tgttattgtt actgaattag atcgattagg acgaaataat aaagaattaa cagaattgat
     2401 gaatcaaatt caaattaagg gggcaaccct ggaagtctta aatttaccct caatgaatgg
     2461 tattgaagat gaaaatttaa ggcgtttgat taatagcctt gtcattgaat tgtacaagta
     2521 tcaagcagaa tcagaacgaa aaaaaattaa ggaacgtcag gcacaaggaa tcgaaattgc
     2581 taagaaaaaa ggcaaattca aaggtcgtca gcataaattt aaagaaaatg atccacgttt
     2641 aaagcatgct ttcgatttgt ttttgaatgg tttatccgat aaagaagttg aagaacaaac
     2701 tggaatcaat cgccgaacgt ttagaaggta tcgagcaaga tataacgtga cagtcgatca
     2761 aagaaaaaac aatgaaaaga gggatagtta atgagtacgg ttattttagc tgaaaaacca
     2821 agccaggcat tagcctacgc aagtgcttta aaacaaagca ccaaaaaaga cggttatttt
     2881 gagatcaaag acccactatt tacagatgaa acgtttatca cctttggttt tgggcattta
     2941 gtggaattag cagaaccagg tcattatgac gaaaagtggc aaaattggaa acttgaatct
     3001 ttgccgattt ttcctgatcg atacgatttt gaagttgcaa aagataaggg aaagcagttt
     3061 aaaattgttg cagaacttct caaaaaggca aatacaatta ttgttgcaac agatagcgac
     3121 agagaaggtg aaaatatcgc ctggtcgatt atccataaag caaatgcctt ttcaaaagat
     3181 aaaacattta aaagactatg gatcaatagc ttagaaaaag atgtaatccg aagcggtttt
     3241 caaaatttgc aacctggaat gaattactat cccttttatc aagaagcgca aacacgccaa
     3301 attgccgatt ggttgatcgg catgaacgca agccctttgt atacgttaaa tttacaacag
     3361 aagggcgtac aaggtacatt ttcactagga cgtgttcaaa cgcccacctt ataccttatt
     3421 tttcagcgcc aggaagccat agagaatttt aaaaaagaac cttttttcga ggtggaagct
     3481 agtataaaag taaaccaagg gtcgtttaag ggcgttctaa gccccacaca gcgttttaaa
     3541 acccaagagg agcttttagc ttttgtttct tctaaacaag ctaaaatagg caatcaagag
     3601 gggataattg ctgatgttca aaccaaagag aagaaaacga atagtccgag tttgttttct
     3661 ttaagtagtt tgcaatcaaa agtcaatcag ctttataaag cgacagcgag ccaaacttta
     3721 aaagctattt cttttttaat aacttaaaaa taaacttaat gtaacagcaa gcacagtcaa
     3781 ggtatacacc tttgacaaaa aatagcacat tctctatcga aaatttttgc ttatttttta
     3841 aattattttg ggaaattttc ccaatccctt tttctaactc aaaaaatata atcactcaaa
     3901 atttaaaagg gcgcacttat acatcatttt aaaaaattga tgtaacgtgc taagttcaaa
     3961 acaaagggcg cacttataca cgattttcaa tcttgtatat ttctaacgaa aagcgtgcgc
     4021 caaaaaaccc ccttcgtcaa ttttgacagg gggctttttg atgtaaaaat ttctatcgaa
     4081 atttaaaaat tcgcttcact catgttataa agacttaaaa taaaataact ctttaaaatc
     4141 ttttgctagt tgttcttcaa tattttttat tcggtgcatc ttccaagtaa agtataacac
     4201 actagactta tttactacgt ttcataagtc attaatgcgt gtgctctgcg aggctagttt
     4261 ttgtgcaagc acaaaaaatg gactgaataa atcagtccat aagttcaaaa ccaaattcaa
     4321 aatcaaaacc acaagcaacc aaaaaatgtg gttgttatac gttcataaat tttatgatca
     4381 cttacgtgta taaaattaaa ttcactttca aaatctaaaa actaaatcca atcatctacc
     4441 ctatgaatta tatcttgaaa ttcattcata aatagtgaag catggtaacc atcacataca
     4501 gaatgatgaa gttgcagagc aactggtata taaattttat tattctcact ataaaattta
     4561 cctatcgtaa taataggcaa taaaaagctg ctattgttac caatatttaa attaaatgaa
     4621 ctaaaatcaa tccaaggaat cattgaaatc ggtatggtgt tttcaggtat cggtttttta
     4681 ggaaacattt ct
//
LOCUS       DAYYBG010000142         4672 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000142 DAYYBG010000000
VERSION     DAYYBG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.147"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(485..988)
                     /locus_tag="ACHAUN_002710"
     CDS             complement(485..988)
                     /locus_tag="ACHAUN_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8461345.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1347..2372
                     /locus_tag="ACHAUN_002711"
     CDS             1347..2372
                     /locus_tag="ACHAUN_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8461346.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2695..3573
                     /locus_tag="ACHAUN_002712"
     CDS             2695..3573
                     /locus_tag="ACHAUN_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM8461347.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4049..4165
                     /locus_tag="ACHAUN_002713"
     CDS             4049..4165
                     /locus_tag="ACHAUN_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8461348.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(4446..>4672)
                     /locus_tag="ACHAUN_002714"
     CDS             complement(4446..>4672)
                     /locus_tag="ACHAUN_002714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8461349.1"
                     /translation="RITDFFEEKKWSELNVRDIGSFRNCLLNDNGYLQPYCSLLHEIF
                     HEIFRKTFDYVVTFPFLTKNVSKTNKGVKR"
BASE COUNT         1549 a          752 c          849 g         1522 t
ORIGIN      
        1 aaaaaataac gacacagaaa ataccgtgtc tcagatttct ctactacttc taaaaactac
       61 tgtttttttc ctttgaaatg ccttcaaaaa gtaggatcag gcagaatata ctggcagaat
      121 caaggagaag ggagcctaaa taaggagaaa aaccatagaa aatataaagt aattacttat
      181 aaaacaagct ttttatagaa aaataatata cctgagtagg gtataaatta agacggaatt
      241 cctttctaga ttgagatatt gactaggaag aatgttaaga ttttaagcct ttaagctaaa
      301 aaaaaaccct ttttgttaaa taaggggaat atagcctgtc agtttcaaac tgcaagtcgt
      361 cctgctgcaa taatatagta gattagagat gaaaatagat ttttttaaac caatgactga
      421 ataaaaagac cgtcatagat acacattgga atgtgtctat tacggtcttt ttttacgaac
      481 gattttattg aagacggaca gttcgggtca aaaataaaaa agctacgatc agaaataaga
      541 ataaaatgta tcccaaaata atggctagac ggtctaactt atttgtcgat agatgtttgt
      601 ttagaagttg ataggcccat aaacctatca gtcctcctgt tgtattagtt atcaaatcag
      661 tgatgtccgc tacgccaata ccgaatagga attgaatcaa ttctgccaag aagcttacaa
      721 ggaaaataat ccctaatttt ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca
      781 ccccaaaagg aaaaaagaca atcaagttat aagtggtctc acgtaatacc gtcgtatttt
      841 caaaagaaaa agtagaaaaa gggacgagat tgacattgct gtaagaataa gtgagaaatt
      901 gtggaagatt aaatgatagc ttgaataaaa tcaaccataa tagaaagaat aaataaatcc
      961 ctaataaaaa ttttgataca taccgcatag acatctcctc catttaaaaa gtataccatc
     1021 gaaaaccgct gaaaaatcat aaaaaagtat gaagtttaag gaccaacgta gttatcgtac
     1081 gaaaattaga agcttttttt tgttgggaat ccggtttctt taacaaagct aagatagcga
     1141 gttgcttgga aaagttgcta tcttttttct gtcaaaacga ctcggaatgt tcgttttttg
     1201 ttctaatttc tccaagttgt ttactttttg atactaagtt tatgaaaaat caagaaatag
     1261 ttgttatact aggtaatgct gacaaacggg attggcattt catcttggtt cagctttttt
     1321 tccaattact aggaaggagg gatagattgt tcaagactta ttttcgtttc aataaaatac
     1381 aagataatcc ttttcgtatc atgaatctga tgctacttat ctttattgta ttatggttag
     1441 gttacataaa tcatgatttt ttgatttcaa gtttttcatc attagggatc tttacttttt
     1501 tctattatca aaacataccg atgaaatatt tgctgaaacg tatgtttctg atcggttgcg
     1561 gactgttggt tgctttcgtt ttggggatct tatcgactta tgttttatgg cttgaaccat
     1621 ttgccgtagc tttggttgct ttttccagca gattcgtgtt gcgattattc catatttcta
     1681 aaccaggtgg cttgtttttt gcgatgcttt ctgccatggg aacgagcatg cagctgccaa
     1741 tcgctcaatt accgatcgtc tcactttatt tttttatggg tgtcgttttt gccctgattg
     1801 cggcagtgat tacaaaatta ctggattcac ggcctgagca gacaattgaa aaagcaactt
     1861 taaaagaaag atttcacgaa gagcctttag tgatcattga ttctgttttc tacagcgctg
     1921 ccttatttct ttccgtttat gtcagtcacg gcttgaatct gcataatcct tattggttga
     1981 ccttgtcatg tgcatcgatt ttgctagcag aaaacttgga tgcgatgaag catcgacaag
     2041 tccaatatct gattggatcg atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc
     2101 cgtttacgca gttgcagacc atttttctga tcacttttct atatggaatc gcacaatttc
     2161 tagttgctag aaattatgca gttgctaata tttttcttaa tccgatggcg ttgatgctat
     2221 ctacacttat tcgtggagca tacttgattt ctttgatcga atatcgtttt ttagggatcg
     2281 tcataggaag tttcatcggt ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt
     2341 atttaacggt tgttcgtcaa aaattagaat agaaggacaa atatatgtgc caaaagaggt
     2401 tatgacaaga tttttgtcac aacctctaat tcaaggtgtt caaaagccag attctcagca
     2461 ttaagtcaaa ttcacacaaa aatgtggaaa ggaactttca aaaaaatttt atatttattc
     2521 tatcaaaaaa ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt
     2581 tccttgaaag tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt
     2641 acaataaaag tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg
     2701 ttcaaagata tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg
     2761 gcagaaactt taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac
     2821 atccttgatg aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag
     2881 gcgactgcaa agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt
     2941 tatctatatg cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga
     3001 actatctatt tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta
     3061 acaggaagct atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc
     3121 tttcggattg gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta
     3181 agattagaag gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag
     3241 aataagataa gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat
     3301 taccattacc aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg
     3361 ttgaaaaaag agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat
     3421 gaacaggacg atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat
     3481 tatccagaaa gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac
     3541 attggctctg gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag
     3601 agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     3661 aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     3721 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt
     3781 tctatcgaac acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt
     3841 aacggacaag agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga
     3901 gataggagtc aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact
     3961 tttgatacca tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta
     4021 caatcaaaga agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg
     4081 ccgcttgaaa agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt
     4141 gaaacggaat agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa
     4201 acgtttttcg gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt
     4261 tgattttcca tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca
     4321 ttccctaatt ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag
     4381 tattatctgt agttattttt caattccctt tcaacctctt attttgacca gtagtattga
     4441 tcactttacc ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac
     4501 gtaactacat aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag
     4561 caatacggtt gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct
     4621 ctaacgttca gctcactcca tttcttttct tcaaaaaaat cagtgattcg ta
//
LOCUS       DAYYBG010000143         4461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000143 DAYYBG010000000
VERSION     DAYYBG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.148"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACHAUN_002715"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACHAUN_002715"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM8461350.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACHAUN_002716"
     CDS             2298..3221
                     /locus_tag="ACHAUN_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8461351.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACHAUN_002717"
     CDS             3237..3776
                     /locus_tag="ACHAUN_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8461352.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAYYBG010000144         4425 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000144 DAYYBG010000000
VERSION     DAYYBG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.149"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACHAUN_002718"
     CDS             690..1658
                     /locus_tag="ACHAUN_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8461353.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACHAUN_002719"
     CDS             1726..2484
                     /locus_tag="ACHAUN_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFM8461354.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACHAUN_002720"
     CDS             2552..3262
                     /locus_tag="ACHAUN_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8461355.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACHAUN_002721"
     CDS             3298..>4425
                     /locus_tag="ACHAUN_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8461356.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAYYBG010000145         4395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000145 DAYYBG010000000
VERSION     DAYYBG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.150"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            516..662
                     /locus_tag="ACHAUN_002722"
                     /pseudo
     CDS             516..662
                     /locus_tag="ACHAUN_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(750..1043)
                     /locus_tag="ACHAUN_002723"
     CDS             complement(750..1043)
                     /locus_tag="ACHAUN_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8461357.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1080..1265
                     /locus_tag="ACHAUN_002724"
     CDS             1080..1265
                     /locus_tag="ACHAUN_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461358.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1446..2624)
                     /locus_tag="ACHAUN_002725"
     CDS             complement(1446..2624)
                     /locus_tag="ACHAUN_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8461359.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2728..3042)
                     /locus_tag="ACHAUN_002726"
     CDS             complement(2728..3042)
                     /locus_tag="ACHAUN_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461360.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3149..3364
                     /locus_tag="ACHAUN_002727"
     CDS             3149..3364
                     /locus_tag="ACHAUN_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461361.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3664..3906)
                     /locus_tag="ACHAUN_002728"
     CDS             complement(3664..3906)
                     /locus_tag="ACHAUN_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461362.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3893..4321)
                     /locus_tag="ACHAUN_002729"
     CDS             complement(3893..4321)
                     /locus_tag="ACHAUN_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8461363.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1388 a          834 c          654 g         1519 t
ORIGIN      
        1 tttttttctc tttttattct aaggttattt tagtcttgtc ctttaataca aagattttat
       61 ttttaaataa aaaatactct ttttttacga ttattgaaaa aaaaagataa aacttgatta
      121 attgaagtat tgcttaaatt tggaagctat gagggaagaa taaaagagtt aggacgggat
      181 tatctactat ttttctttgt tttaaatatt tgttatcttt gaacataaaa gagaaatgtg
      241 tcgactgcag tgttgtgtcc ctcatcacga ataaacggtg ttcaactagc tgatcttgta
      301 ttcacttaaa aaatggtaaa gtacgagttg ctattcccca ttttcaattt tctaacgctc
      361 aggacaaaat actgttttca caacctcatt ggaataaaag catatatatt gtttcaagaa
      421 cgaaatcttg ttatcttttt aaagggcatc tgtccaaaat ctaattttag gaggtacgtt
      481 ttggtattta ttcgtacaaa taaaagagaa ttatctttat tcatgtaacg catccaattg
      541 attttttcat gtacgctcct ggaacgattt tactgacgat tgcttattta actgcgaatc
      601 gctcattagc ttttatcata gctatccata ttttgaataa cgttcttggc tttgtattat
      661 agatacagat gacaaaaaaa gttcaagtat gcggtgtaga accaatatcg catacttgaa
      721 cttttttcaa tgagtataaa ttaaaacttc taattttcaa ttcccatcat ctgatatgcc
      781 cgaccgtctg aacttctttt gaaaaagcca aatgtgatta gataacgtct ttgttcgaca
      841 taatcgccac tagaaaatac gttcagcagt atcgaattaa cctctgtttc tgtataagtc
      901 actccatggt caaagcgttt caagacttct ttcaatagga tgtatttctt ttttgttgaa
      961 cttggtactt gttttacttt tccatcattc atgaatgtgt ctaaaacttt catcttttct
     1021 tgctccgtat aagtataaac catcttcttt ctccttttga ttctaagaat actccattta
     1081 tgttagaata ttctggattt tttattaaag cggttactct taagaaagat attatcatag
     1141 tgctcttttt tttactacta cttataggta gatttattct aatccattat tctcccctac
     1201 caataattaa agaaaaaact ggatcagggt gttcaaatta catgctaaga aaaaagaacc
     1261 cttgaatacc aagggttctt tctcagtctg ataacaatga ttaacgacgg atttctttga
     1321 tacttgtgaa aagtcgttaa agtattgata taaaaggatt tttattacct ctgactacat
     1381 attgactaat ttgttaaaaa atcgtttttc aagaatcctg taataccagt gctttcaccg
     1441 tttttttaac tctctaaaga actaaaagca atcagaggtt ttaccttttc atttgacgtt
     1501 aagtggcgat aaattttcga agttgtttgg tcagtatggc ctaagcgatc tttcaagact
     1561 tcccagctac agtgttccgc tactaagctt gccattgtat gacgagctaa atgagtatga
     1621 aatggcttat taattcctga gcgattcacg caccgtttga cgtgatcatt tacatactct
     1681 cgacgataag gaacaccacg tttactagaa tcaagataca caaacaaata tgtttcttct
     1741 ataataaacc tgtaatgttg cctcatctca cccaaagcct taatcaactg ttgtagcttt
     1801 tccataacta gaggagttac tagaactcgt ctagcagaac taactgtctt agtagtttca
     1861 attcgaaaat ccttactctt acctttaact gccgtttgct tatagaacag aatttcattt
     1921 cttttgaaat ctatatctcc aggctgtaga gcaagcgcct cgctgactcg gcagccagtg
     1981 taaaatagaa catggaaaag tacagacatc ggtaattcct catgaacaat gccataattc
     2041 aaaaaagttc tcaattcatc gacagtaaga aacttgtcct caatattatt tatggcttct
     2101 tttaaatcag ttaccgttaa ctgatatttt ggcattctca cagacttcat tggattttca
     2161 cttatcaaat tctttctttg acaatattta aaaatcatat ttagaacttg aataagtgat
     2221 tgtaaactat ttttggcata gccaacattt gttccattaa cgccacattc ctttaaggta
     2281 aaaagcaatt cttgaaggca ctctggtgtc aaatcagaca actttttatc accaatccat
     2341 cgaacaataa tttttaattg ttcctctcgc ttatcatagg ttatttcctt ttgttcattt
     2401 aacgaacggt aatattggaa ccattgctca cctacaacac taaacttgtc attgcttgca
     2461 acaatttttt tcttaagcct ttgttgtttc tccatttcca gataagcgag ttgcgcctca
     2521 cgttttgaat taaagccacg tcttgttgtt cgatatgctt gaaagtacca tgtgatccta
     2581 ccagtagaac tgatttgctt cttgaatcga tctgtttttt ccatttattg attcctccta
     2641 ttttgaagga acccaacaag cagaaaccct ttggaaatct ttctatctat gtcatcattg
     2701 taccgcatct tagaaagaaa atgtaactta taaagatcga agtagtcgca atttttctcg
     2761 atagtcattc gaatcactct ttctcatcca ttctaaaaca tcatcacgaa aatagtgtcc
     2821 tcgaatcccg taccaacagg gaggaaactc accaagtttt actaaacggt tcagtgtact
     2881 atcacttatc tgaaatagtc gctttaaatc agtatttgtt aataccagag gtaaatgact
     2941 gactgcaagc aatttttccg tgtaatcttc cagttttttt tgtaaaattg aatagaactc
     3001 tatttccaga gaattaacat ttgttttaac ttccaactcc atcacccttc tctctcaaaa
     3061 tcaaaacgac caccacggta ccccttaaaa ttgtaaaaaa acactccagt actccatcaa
     3121 aacggactag aaaagaggaa tttttaatat gaaacaagga attgcagaca taaaaataat
     3181 taaagaaatt cttgaaaaat ctacggctaa tgcaatagca ttcggaacag gaatcaactt
     3241 gagtacagta aaaaaactta aaagtggaga gagagctgaa gaaaaattaa atcttgccga
     3301 tgctattaaa atcacagaat ttggcatgaa aaatatgcca accaaaatcg aaatatggaa
     3361 atagtagttt ttctgatcta tcagctgttt tgccaaagca cataggaatc tcacctcccc
     3421 ccagtaatgg cgggccgatt tggttacgga agtatcatta tcgtagtcgc gattctcata
     3481 ttaaaatttg tcgcttaaaa gctaaccttg tcatggcgta actagagaat caaaatctac
     3541 atgtactgat agatttgcta ttcatttgtc aaagagcgct aggctatttt cagcctatgg
     3601 aaatccttta ttttgacccg ataaaaaact tccatagaat gaaaaggaaa gggaaaaaat
     3661 actttaacga gttgtatcaa attcaagtat cttctcaatc aattttgttt ccagttgtct
     3721 acgcatatat tcatctacat acataaccat tcctttttct tcttcataaa ctggtttcat
     3781 ggctaaccgt gaaatataat tttgatattt tgacagaacg atggacatgg caattgaatc
     3841 accagaagct gcggctttga ttgtcgttag tggtatgaat ggtatatatc gtttattttg
     3901 cttcatattt gacacccaaa tactttccta gctcttccaa agccatttgt cgttggtacc
     3961 aaacactgcc gagacttttc ccaataatat cgccaatttc acgatcatta agaccgatga
     4021 agaaggacat aaaaatgatt tcttgtcttt gactaggtaa tgctgcaatc gcatttgcca
     4081 actcctcatt ggaaataaag attttcatac caaggacaat aaaagtacca ctactattca
     4141 aatttggttc caaataatag aactctgtac ctaacccttt ttgttccaat atttccattg
     4201 atatttgccg ttggcgaaat ctagcgtact tcttttgaat atttaacgcc tcattgcaga
     4261 ctactttctt acaaaaactg tcaaacgtat gttgaatgtg ggtttcctct gagtcgccca
     4321 taatattctc cccctcaaaa aattcattca actcgtttta cttctatccc tttccattat
     4381 tactacaatc tagga
//
LOCUS       DAYYBG010000146         4377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000146 DAYYBG010000000
VERSION     DAYYBG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.151"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACHAUN_002730"
     CDS             73..234
                     /locus_tag="ACHAUN_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8461364.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACHAUN_002731"
     CDS             253..1530
                     /locus_tag="ACHAUN_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8461365.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACHAUN_002732"
     CDS             1540..2196
                     /locus_tag="ACHAUN_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8461366.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACHAUN_002733"
     CDS             2196..3572
                     /locus_tag="ACHAUN_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8461367.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACHAUN_002734"
     CDS             3702..4115
                     /locus_tag="ACHAUN_002734"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8461368.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACHAUN_002735"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACHAUN_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAYYBG010000147         4355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000147 DAYYBG010000000
VERSION     DAYYBG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.152"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1383
                     /locus_tag="ACHAUN_002736"
     CDS             82..1383
                     /locus_tag="ACHAUN_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8461369.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1410..1670
                     /locus_tag="ACHAUN_002737"
     CDS             1410..1670
                     /locus_tag="ACHAUN_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8461370.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2669..3904)
                     /locus_tag="ACHAUN_002738"
     CDS             complement(2669..3904)
                     /locus_tag="ACHAUN_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8461371.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1272 a          694 c          894 g         1495 t
ORIGIN      
        1 taattatata attagttatg aacattcttt gcgttgtcat gcaatgaatg gttcatattt
       61 tattgaaaag gagtggtttt agtggaaaag ctgatgaaat gggtcgaaga gaaacttgtc
      121 ccaccaatgg caaaaattgg tacacagcgt catttattag caattcgtaa tggggttgtt
      181 tcaacattgt cattgatttt gattgggaca ttttttatgg tctttatcaa tttaccgttt
      241 cctggatgga atgaatttat tgccccttat tcggcaacga ttgttttacc ctttaggatg
      301 acgatgggat tgatggctat ttatgcaact tttgttatgg gttcggatct agctaaatca
      361 tatggattag atagtgtgac aggtggtata ttatctttag gaacgttctt tatgttacaa
      421 gttcctgtaa acgttttaac tcctgaagag gcacctcttg gctgggtctt acctatgagt
      481 tctttgggag catctgggat gtttgcgggg atcttatcta tgatttttgc agtggaagta
      541 tatcgtttct ttaaaaatcg aaatattact atcaaaatgc cagaacaagt gccacctgct
      601 gttgcccgta gttttgaggc attgattcca ggtgcagtca ttttaacaat tacttggctg
      661 attcgaagcg tcgcgggctt tgatgtaaac gctgccttat tatcgttatt cgagccttta
      721 accaatattt tagggaataa tttgcttggt gttttactac caatgttttt gatccatttg
      781 ttgtggtcat ttggtatcca tggcatgagt attgtagggg ctgttgttcg tccaatgtgg
      841 ctgatcatgt tggatgagaa tgcaaaggca ttggcagatg gtacagctgc cacccaatta
      901 ccctatatcg ctcctgaaca gttttatcaa tggacggtaa caattggtgg tgcaggagca
      961 acgatcgtgg tatctgtttt gtttatattc ttctgtaaat caaaattctt gaaagaggtt
     1021 ggtcgttttt ctattattcc tggtatcttc aatatcaatg agcccataat ttttggagct
     1081 cctatgatgt tgaatcctta catgttcatc ccgttcaact tagttccttt agttttgact
     1141 attatcagtt attttgcagt caaattagaa atggtcaata gatttactgt tttgccaaca
     1201 tggacattac cagctccaat cggcggttat ctttcagcag gaaatgactg gcgtgttgtt
     1261 atcttgatca tgatcaacac attgatagca tttattatct attatccatt tgtaaaagct
     1321 tatgataaaa agatgctgtc agatgaacaa agtcgtggag aaaacgaagt ccagactgtc
     1381 taagtttggt ttttagaagg tgggaaacaa tgtattggct gattttattt tttgttttta
     1441 tctttttatt aacggctagt caccttatat tgaatatgct agctacatat catattcaaa
     1501 tcaatcgatg gatttgggca ctggcatcat ttttgatcgt gattcttcca aagatcattg
     1561 taccgcatat gaatgttttg ttttcatggg ggacatatgt actttgtgga atttttgcta
     1621 tcaattttat gatcgagcag catagatggt ttgtcacatc gaagctttaa atgttctaaa
     1681 agtggttgta acagaagtgg ttagcttcaa gaaataagaa gaaatttacg aaaattgctt
     1741 cttaaatttt tgtgaaatgt tggcttattt ttcgtttggt tgaacttatt accgcagatg
     1801 caagcttttg accaccgttt attcggaata tgggactgtg acaaggcttt tgtcacagcc
     1861 cttttttaca taaagattat tattgtttta actgttttgg cagaaagaag gaggaaaaaa
     1921 tggaaatttt atcagcaatg gatttagaat tagtagatct actaccaaag tgatcaagtc
     1981 aaatatagat ttgggagctt acaaccatat agtaggggct gcagtatggt gtatgaatgg
     2041 taatatgtat gatgtgagtg tttattcaaa tcatgaaacg tgtatagaaa taattgtgct
     2101 gggaatagca atcagtcatg gtgaacgtga cttttagtgt atcgttgctg acgacataga
     2161 acattctgac atgatctact caatatgtgg ggattgctgg cagtttatat aggagtatgc
     2221 atcggattgc gagatcattg tctcaacaga agagggaggt tgattatcaa agataaagtg
     2281 gtaaatccta catttgattt agtaattgtt gaggggacta gcggcacaaa tctctacaaa
     2341 gcgatgcttc tagatctata tgacacattt taagaaaatg tatttgccta attattatgg
     2401 tctttctttt gaagaaacgt tgagatgatc caaccagaga aaggtgtcaa agaatttgac
     2461 taagaataaa tggcagacgc tacctatgct catttggata gcgtcagatc ttttatttta
     2521 aggatgattt tgtgtttttt agacaccgta atttaagtaa ctctaacttt tcaatccact
     2581 atttactttt tttgtatttt cactgtctgg gtctcgaata gcgaaattac tgatgagtct
     2641 ctattaaaaa agctttttct acttgaaact atattgggtg gttgctcagg aaggtttcga
     2701 tttcttgaat tgcttgttgt ggaccattta ttggaaaaac ggcttgcagc gtgcgtgcct
     2761 gctgacgaat cttgggtgaa gaaacttcgc gcagagcaat ctgcagtttt ctttcagtca
     2821 ctggttttgt gagggcgatt gcgtttccgt attttacaca atagtcaata ttacagcttt
     2881 gctcaaattg catccccaca ccgataaacg gtttgccgga ttcacaggca gtttggacgg
     2941 tcccttcccc gccgtggata aaagaaaaat caatgaattc atgcaaaaga tgactagaca
     3001 gatattctgt tattaaaaga tcttctgagt cagattgaat gtcatcaagc agatagtcaa
     3061 taggtgcaat aaccgctaga tccggttgtt tttgtaagaa gaacaaaatt ttgcgtaaca
     3121 tacgcggatt gcctgaacta cccattgcga aatagataat acgttttctt tcttgttgct
     3181 ttcgctgtat aaaagttagc acttcttcag gcaaggaagt actgagctta gcaaaaattg
     3241 gaccaacgat tcgataattt tccggcagct gttcttcttc caaaaatagt ttaggcgttg
     3301 tgatgagatt tagatcagca tccatcattt gtaaacttgt ttttggtaaa ggaagcattt
     3361 ctttttgtgc agcgtgtttg aaggcattgg ggatccatcg ggtatttact atacgctttt
     3421 ttgtccagtg ccaaagggga ttgcttaaag ggccgaagaa acgtaactcg tggggcatca
     3481 gcgcatgctc tgattgaaaa aaaggttcac tcaaagcata gggtttaaca tatactaaag
     3541 gaatcttttt attgcgggcc gataaaaaaa ttgtcacatt cgaacctgtc acaatcagtc
     3601 gaggctcaaa cttagcaatc agactctgtt cactagtgat acgagaggca accatttctt
     3661 tggtaaaagg gtttttaagt gtacgcaact ggtcaaattt tatgatttgt tcttcttgtt
     3721 ttttcgtaag gatcggcgct agtaaatgat actcaaagcc actttctgtg atcaaaggag
     3781 caaaagtttt agaaaagcca aagaatacac agtaataacg gtctttcata gctttggcaa
     3841 cctcaatcat tcgtgttgtc tcagctaaat tgaatactgc aggagcaaaa agaattgtct
     3901 tcatgaaatc ctccttattg gaatgagtct atcccttcgt ttcttactag tatactaaaa
     3961 aacacagcgt tcgagtagcc tatgtatatt ttgaatatta aactgtctct cgctgttttc
     4021 acaacttcta aatttaaatt ttgtaatcaa gttgaaactc caattttcaa aaaaacacgc
     4081 aacaattttt tgatcgattt atatgttgtg ccagataaat acgagaagtt gtgtcattaa
     4141 aaataaacat gagaacataa aaaaataaaa taattatatt aattgatagg tgtgctgtcg
     4201 tcattataca cacgtaagaa agaaatagtt atgtttaaaa atattcttag actttctcgc
     4261 catgacaatt ttcactatgt gttataactg taaacgataa ttattcagat aacacaaaaa
     4321 gaaatcaagg agcagatacg ccagttgttt aattt
//
LOCUS       DAYYBG010000148         4295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000148 DAYYBG010000000
VERSION     DAYYBG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.153"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACHAUN_002739"
     CDS             <1..792
                     /locus_tag="ACHAUN_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8461372.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACHAUN_002740"
     CDS             889..3138
                     /locus_tag="ACHAUN_002740"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8461373.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4295
                     /locus_tag="ACHAUN_002741"
     CDS             3141..>4295
                     /locus_tag="ACHAUN_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8461374.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1394 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctaga
//
LOCUS       DAYYBG010000149         4282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000149 DAYYBG010000000
VERSION     DAYYBG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.154"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACHAUN_002742"
     CDS             complement(37..1257)
                     /locus_tag="ACHAUN_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8461375.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACHAUN_002743"
     CDS             complement(1277..1888)
                     /locus_tag="ACHAUN_002743"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8461376.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACHAUN_002744"
     CDS             complement(1931..2863)
                     /locus_tag="ACHAUN_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8461377.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACHAUN_002745"
     CDS             complement(3284..4144)
                     /locus_tag="ACHAUN_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8461378.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAYYBG010000150         4224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000150 DAYYBG010000000
VERSION     DAYYBG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.155"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..461
                     /locus_tag="ACHAUN_002746"
     CDS             24..461
                     /locus_tag="ACHAUN_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461379.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            556..1440
                     /locus_tag="ACHAUN_002747"
     CDS             556..1440
                     /locus_tag="ACHAUN_002747"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8461380.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1662..>4224
                     /locus_tag="ACHAUN_002748"
     CDS             1662..>4224
                     /locus_tag="ACHAUN_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8461381.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1377 a          711 c          957 g         1179 t
ORIGIN      
        1 aaaatgcacg tagaagtaga taaatggagt ggttttgcaa atttctttta tttctagaag
       61 ttattcacgt atacggattt gtttttctca caaataaaaa ggcaggaagt acaatggatc
      121 atttgtatat aaaatatatt catcaatttt ccgttttggt attaggcaca ctaattatag
      181 cggggattgt tgcatttatt attcaaggtc gtgacatatt aacagtacat caagagtttt
      241 tcagttatgc atttggtatt cctctgatcg ctataaggtc tgataacaaa aagtggaaga
      301 aacgctatta cattctcttg ctatttttgt gtttttatct gccacctaag atttttactt
      361 tattaggtat acaatgccta tcattttctt ttatttccta tatcattgtt ttaattttcc
      421 tcagtttcat agtatgggta ttttatcttg tcaaaaaata aatgaatgtt tcatgaacag
      481 tacttcatca cagcctaatt tttttcttta tgctatcgtt atattaaaga aatcaatcag
      541 aagggaaaga aagtgatgaa aaaagtttta ttagtggtaa accccagttc aggcggtgaa
      601 caagcaaagg aatttgagca attagcgata gcaaaattgg aatcggtgtt tgatgaagtg
      661 gtcgtcttgc atacaaaaaa agcaggggat gcaaaaaact ttactcgcga agcggctacg
      721 gaagggtatc atagtgtgtt tgtcatgggc ggagatggaa cagtcaacga aggaatcagt
      781 ggaatcgctg aacaagaaca tcggccaaat ttcggatttt tcccattggg tacggtgaat
      841 gacttggctc gagcattagg gataccgcta gaacctgagg aagcgatcaa tcatttttcg
      901 attgaatcag tcaagccatt ggatattgga aaaataaatg acgactattt tatgaatgtc
      961 gtcgcaattg gctctatccc tgaagcaatc aatgatgtag atgcggagaa gaagacgaaa
     1021 tttggaaaac tagcttattt catgtcagga atcaaacagt tagcttccac acaatcctac
     1081 tcgtttcacg tagaagttga tggaaagaag gaagaaattg agagcagtat cttgctgata
     1141 ggactaacta actcagtagg cggttttgaa acattacttc caaatgcaaa agtggatgat
     1201 ggtaagctcc atttggttta tttaaaagat tcttctttga tcgatacgct taaaacattg
     1261 ccagacctat taaaaggagt agatgaatct acgaaaaatc tagcctatct tacttgtgag
     1321 caattagttg tgtcattagc tgatgaggaa gaattgacga cgaatgttga tggagatgaa
     1381 ggggataaat tacctgtgac aatcaaagtg ttaccctcac atttacaggt ttattgttaa
     1441 aatggaggtt gtgaccgaag agttgtcagg aggacatcgg ttgttcggaa taagggactg
     1501 tgacaagact tttgtcacag cctttttttg tacaaaaaag aaattttcac ctaacagaaa
     1561 aatttgccat aaaaatacat attttaaata tgaaagtaca attacaaata aattaaaacg
     1621 ttaaaataaa attgtcaaaa cgtcagagag gatggtggag aatgaatcaa tatgtaaaaa
     1681 agctgattgc gtcgattttg tttgtcttcc ctctcttgat ggtgacatcc gtttttgccg
     1741 atgtagagaa aacaagcgaa tcaacgcaat cagagaatca aagttttgac aagactactt
     1801 tttcagaaga atcaattact tctgaaacta cagaatccca aagtgaggcg cctacttcta
     1861 atacaaggca agaagaaaat aagacaagtg agacctcaga agtagctgaa caaacgtctg
     1921 atagcagtga agaggtacag gttagagttg tttcagcgga tgggtacagc gaagctgcca
     1981 caacagaaga actgacgcag cttctcgcag acgaatcagt agctaagatt cgtttaactc
     2041 agcctttgac tttagacagg gaattggaga tcaagcgaga tatcgttatc gattttggcg
     2101 gacttgctca taatttcggc acacatcaca tctatatcaa tgagacacct cagtcgatcg
     2161 agtttcagaa ttttaaagga acggcagctc atccaggtgg gttgatacca aatatcgatg
     2221 gaaatgccat tatcattgct tatatgtccg atttttgggg aaacagatat cattttacgg
     2281 gagaaataaa atttaccggg acacttgact tatatgatgg ttctaaatta ggcctgattt
     2341 acgcccctcg tgcaactgtc acattagatg gtgtttccgg tgtattggat gtccaaccag
     2401 tgaaagaagg gatgaatgcc gctccgggta aagcttattt ttgtagaagc tatcagctaa
     2461 atgtaatcaa tggtagtcag ctttatggac catatctagg aaaattctat ggttttctag
     2521 attcaggtga tgagacgggc agtggaaaaa cagcaccagg actccatatc ttgagtggtt
     2581 caaaggtctc tcttgactat gatcgggcag atggcgtcag cgatgaagga gaggctgtcg
     2641 atacgctacc cgggaatgtc acatttaaag tcagtggctc aggaagtgaa ttttcggtaa
     2701 atacaaagat caatattacc aacgacaaca atcgcggaat tatccaaatg agaggctcag
     2761 gatcaagagt cgctgtgtcg aatgatggga aaatcacaat caatacagcg actactggcg
     2821 gatttcgcct gcaaggagat tctagtcaaa tccatgtatc aagtggtgga caaatctcgg
     2881 tcaaaatggc tggagatggc gaccaacctg gaaacaatgg gatgcgtttt gtagggaaag
     2941 gactttcttt gatcgtagaa ggagccggat cagtcatcga tattgataag caaagtggtc
     3001 gttctagcgc agttcgtttt gagaacggta cgcaaaaact aacagtaact aatggaggct
     3061 cattgaaggt aagaaatgca ggggatggca aatcttatgt gaacagactc aatcaaggaa
     3121 accaagcgat ccaattttat gacgcttctg gttttctaga aagtccaggg tcagctgatt
     3181 ttacggttac tggagaaaaa tcaaatatcg atatacgcgc agattccgga gctaccgtag
     3241 atacaactgg agatattgat ttgaacttta tggctggcga aaaaacttat ttagtcatgg
     3301 tcgggaaaac tggggaagat aagactggca tcatcagtgg ggatgtgttg aatgtagact
     3361 tagaatctcc tacctatttc gatttccaaa acaaacggat cggaagcgag actaatggca
     3421 taggtggctg ggtatttcaa ggaaatggcg attcttcttt gaagatcgct cattcagaaa
     3481 ttgctttatg gcgaaaggca ggagtaacgt tcgataactt tgaaaaagat ccggattatt
     3541 tttctgatat gacagactta tcgatcaatg gaaccgactt aggtaatttt gtatcaagcc
     3601 ctagtgcagg tttaacaaat gaatttgctc agacaggtgc cggaatgaaa aattataatc
     3661 gaatcagtgc caataaccag cttccgatga ttgaatcctt aagagtacca acgaatgcag
     3721 ataaaaaaat ctatgggcat gtcgttgttc ccgaaggtgt cgaggcaaag ccaagagatg
     3781 cttggacagg cgaagtagaa gtgactttac gtattactta tgcagatacg acgaaatcac
     3841 ctgtagaaat tactgctctc acacaaggac aaacagatga aaatgaaggg acaggatata
     3901 atccgtgggg agaaggcgaa caaggcggtt tgtttgaggt tcaagtaccc aacgaagaaa
     3961 gactaacaac gggggatcaa gtgcaaatag ttgctgctaa aaaagtaaga ggcggccaag
     4021 aagtgacaga actggactct gccaaaacga cagtcgatgt gattcctcct gagcctgcta
     4081 aattaagtac cacattgatc aatgcagctt ctcgctcaat cacaggtaca ggcgaagcag
     4141 gagctttggt ttcattgaaa aaaaatcaag ctgttttgtc cgaaacaact attgatgata
     4201 caggtaaatt tgttctttct atcc
//
LOCUS       DAYYBG010000151         4167 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000151 DAYYBG010000000
VERSION     DAYYBG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.156"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..370
                     /locus_tag="ACHAUN_002749"
                     /pseudo
     CDS             21..370
                     /locus_tag="ACHAUN_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(642..932)
                     /locus_tag="ACHAUN_002750"
     CDS             complement(642..932)
                     /locus_tag="ACHAUN_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461382.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1334..1570
                     /locus_tag="ACHAUN_002751"
     CDS             1334..1570
                     /locus_tag="ACHAUN_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461383.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1577..3529
                     /locus_tag="ACHAUN_002752"
     CDS             1577..3529
                     /locus_tag="ACHAUN_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8461384.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3601..4098)
                     /gene="dfrG"
                     /locus_tag="ACHAUN_002753"
     CDS             complement(3601..4098)
                     /gene="dfrG"
                     /locus_tag="ACHAUN_002753"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8461385.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1420 a          731 c          711 g         1305 t
ORIGIN      
        1 gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa atctacgata
       61 gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg acctatggat
      121 tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata ttgcttttaa
      181 aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag gtttaactgt
      241 cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt acgactatga
      301 taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat cctacaattc
      361 cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc cccattgagc
      421 gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc ctcaaatgag
      481 tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata tatagattga
      541 ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc catctttcca
      601 aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat tctttcatct
      661 tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat tttgggtttc
      721 cctaacacat gataggatct cacaacaaat tgctttctat tattataata acctgtaacg
      781 actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga agcaatcaaa
      841 caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg caaagtacgg
      901 atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc attatacgaa
      961 catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt tgtctattac
     1021 ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact aaaaaaaaga
     1081 ggcagcagta tagatttaag tagttcagtt ttggagtaca aaaagtgaaa atgtaaacct
     1141 aaatgagtat caaattgcaa ctgaatttga tactcgtttt ttattgttaa atcaacgttt
     1201 gttgttaatt tacaccacaa aatgtaagtt aaaatgtata taaaaatgcg aacgtgaaat
     1261 aattattgta ggaatgcaaa ttggtgtgtt aaaatggtta caatttacta agcaaaaagg
     1321 ggtgcatttt cctatgcaat ttcttgagcc aaaaaataag aacgctaagt ctgtggattg
     1381 ggaaatatca gagcaagtaa gggtgatagt taagcaatac gctgaatatg cagagcgtac
     1441 tgaaagcgaa gcagtggatg aattcttatt gaacatttta gacgataaga agtttattga
     1501 atggattgcc aacaagcgaa gtaataagcg gattgttgaa aaaatgggta tcaaagatag
     1561 agtgggttga tagacaatga ccaagcttaa aagattagcc acgaaggaag atgaaattgt
     1621 agatgttaaa attccgattt caaacgaaga attaaaagat agggctaaac aatatgattt
     1681 attaactcca aaacgatttg caacaaggta taacaaaatg ttgttcttgc caacgtcttt
     1741 taaatggaac ggttctgaat atcccattca atataattac tgcatcaatc cgttttgttg
     1801 taattttggg aaagaacagc acaaattcaa agatgtaaaa gggaaaccaa gtcgatataa
     1861 aatgactggt agttccaaag ataaaggtca taaaggtatg tactgcaatg acaatcctat
     1921 tggcagaggt gtatcgcaaa actgtacagt aaccccttta tctaattggt ctgttgttga
     1981 agaaattaaa cgccttatag aaatcaatag catccaagat gttgagccag attatcaatt
     2041 tcataaagaa ggctgctcgg aagaagaatc aaccccattc aatgagccga agcaatttta
     2101 caaaaggggg aaaagtaggg gcaaatctca acgctatcaa tgtaaggcgt gtaagaagtt
     2161 cactaatgtt ctaccaaaaa gagaagaaac cactacttat caccaacaaa agaacaccat
     2221 tcttccaatg ttcgctaaga tggttgttgg aagggtttca gtcagtcgta cttgtgatat
     2281 tttaggaatt ggagttggaa cctactatca taagctggag tggctgtata ggcgttgttt
     2341 ggagttttta gaacgatatg agacacaacc ccttcaaaca aagaaattta acgagatgtg
     2401 gcttaatacg gacaagatgc actactacct aaataacgtc cgtaagaaag gacaaggttc
     2461 aaagaaatat acggggtttg aagaccttaa tatgcaaacg tatatagttg tatctgctga
     2521 ggtcttatca agatatgtgt tccgttctga tgttgcctat gattggaata tttcaatgga
     2581 tgaattaaat gaagatacaa gaaaattcaa agaagaccat ctcaatactt ttagtcgtaa
     2641 aaatgatagg ttagattggt cttattatcc acaagaacca tccgctaacg atagcgaaaa
     2701 taggaatgct tatcttcacg aattagggaa aattactaat aggagtagat ttgtcgatgg
     2761 tttaaatgtt gatgctcctt atactacaac tgctcactat tggttaatta aacaaatggt
     2821 taatgccgac gaatggcgaa tgattagtga tgatgacttc tctattagaa acgcctttta
     2881 tagagtattc acgaaagaat taaggttgag cgatgcacat cattttattt gccaagttaa
     2941 caagacgaaa agcagaaaac aatgcttaaa agaattcgga caagcaaaag cagaactgct
     3001 cgattgggga gatatcagag gattcaaaac aaagtttcta cggactttgg catctcatta
     3061 tctaactgaa ttactaacaa gtcatcaatt ccacgaagag gctattagta aagatggaga
     3121 aagatatcgc aaatatgccg ataaccctat caaacatcca ttagccacta aggataaagg
     3181 attttattca gttgattgta gaacggattt atctgctctg gaaccaaacg aaatcgcaaa
     3241 gatgctatta aatgttaatg accattcgac caatagcttt attcaacaga taagacgtta
     3301 tatatcatct cttgaaagac cattaacaac agctcgtggt gataagaaga gctatattta
     3361 cgctaacttc aatccgaagt atgctcaatt tgcgattaca attctaagaa cttactacaa
     3421 cttttgtcgc ccttttaaat cagcggataa aaaggttttg acacctgctc aaagattagg
     3481 gattacagat aaacaatttg attggaaaga tataatttat ttcaaatgac aaaaagagct
     3541 tggagtaatc ccaagctctt gtgctttatc tttactgttc ttcttcaatt aacgcacccg
     3601 ttaactcaat aagttttttc tttcatatac atgaaaatag tagttatacg gatttttatc
     3661 attctttatc cctttttggg caaatacctc attccattcc tcataattca cttctggaaa
     3721 aaaagtatct ccttcgaatt catgatgtat ttttgtgatg tacattttct caacataagg
     3781 gaaaaacaaa ttataaatct gttctcctcc gaaaataaaa atttcttctt cgtttttaca
     3841 taactcaaaa acatcttcta ttgaatgaac aatttcacaa ccattaaagg taaacccctt
     3901 atctctcgtc agaataatat ttcttctgtc aggtaaggct cttccgattg attcaaggtt
     3961 cttcctacct aatattatcg gatgtccctt tgtagtattt ttaacatatt cccagtcctt
     4021 gggaatcctc caaggaatgt cattctcttt gccaatcact ctattcttat ccatcgcagc
     4081 aatcaaagaa actttcattt ataacacagc ctttttaact aatttaccat ctaatttctt
     4141 atttctatca aaaaaccttt tctcatt
//
LOCUS       DAYYBG010000152         4062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000152 DAYYBG010000000
VERSION     DAYYBG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.157"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(171..1676)
                     /locus_tag="ACHAUN_002754"
     CDS             complement(171..1676)
                     /locus_tag="ACHAUN_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8461386.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1673..2359)
                     /locus_tag="ACHAUN_002755"
     CDS             complement(1673..2359)
                     /locus_tag="ACHAUN_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8461387.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2552..3973)
                     /gene="gndA"
                     /locus_tag="ACHAUN_002756"
     CDS             complement(2552..3973)
                     /gene="gndA"
                     /locus_tag="ACHAUN_002756"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8461388.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1117 a          857 c          693 g         1395 t
ORIGIN      
        1 ttttattgtt ttttcgttca tctaaaagat acttacattt aattaacgga tgttattcta
       61 tttaattgtt cgctagtttt tttataatga catccctgaa tttctcactt gcttgtattt
      121 tgtaccttat tctttgttgg atatataaaa aaacaagtaa agcacatgct ttacttgtct
      181 tctgcctttg cgataggaat aaagattcta aaaattgtcc cttgccctac tacactttct
      241 gctaagatac gtcctttata gttttcaacc agttgtttag cgatagacaa ccccagacca
      301 tttccaccct tcgttctagc tcttgcatta tccactcgat aaaaacgatt gaagatctta
      361 ttaaggtctt ctttgggtat tccttcgcca aagtcctgaa tcgcgatctc aaattcattc
      421 agagtggaag aaatagagat atggacttct tttcgtgtag ttgaatactt tactgcgtta
      481 tccaatatga taatgattaa ttgttcaaag tgattacgat aaatttgcag ggtcacttct
      541 tttgtcaaat catcatctaa agtgatcaca tagtctggat ataatatctt gaaattatta
      601 taaacttgat aggtgacttc ttttccttta gttgtttcat tcgcataatg aacatctact
      661 tgttctgctc ttgataagtc tagcatctct tgaactaagc ttttcatacg gctgatttct
      721 tgcatgctgg cgcttaatga ttcttctaat atctcaggat catctttccc ccaacgattt
      781 agcagactaa gatgcccttc aataattgca acaggtgtcc ttaactcatg agatacgtct
      841 tccacgaatt gctcttgttg ttcaatatac aaacgcatac ggtcaagcat ctcattgaat
      901 atttcagcta aatctgccaa ttcatcattt gtgtcgattt caggcatgtg gatatctgat
      961 tgcggatctt tacgtatagt atccattgta tctctgagta ccttgagtgg ttttaagaaa
     1021 taagaagata aaaagaaacc taaaatacta cttaagatca aagaaacaac ttctaaaaca
     1081 ataagagtca ataatagctt atttcgaata tcatagaaag aagataattc atagaagctt
     1141 tgcgcatagc cgatcttttc tcttgtacct ttagaatata taggctggat agataaaaag
     1201 ccagtttttc cttctagtgt aacgatcgtt ggaactttac gtgtagtctg aatcaggttt
     1261 cgactgtttt catgggtttt aaaaatcaag ttctcgtcca aatcataaac ataaagatcc
     1321 attgatggct gccctagctc agctataaag gaatcgatct tcattaagtt tccttcgacc
     1381 agtgaagtat gatctacgac ctcatctccg tcatttgtcg ctttcgtcag attacgatac
     1441 gtatttacta atgtcagttc ttcatccgag ttagccaaac gagaagctac ttctgaaatc
     1501 gtacgctcta cattgtttct ttcttttgcc acaatcagat tgatagatga tttataggta
     1561 atgacagcga aaattgtaaa cacaacgaat atgaagaaag aactcgcaaa ggcccacttg
     1621 attgtcaagg agggcccttt cagttctctt ttttccgatt ttttcggctt tatcacgagc
     1681 gcatcacata acctgttccg cgtacagttt ggatatagct ttcctccccg ggaacgtcaa
     1741 ttttgttgcg aagataacga atatatacgt ccactacgtt tgtttcaact tctgtttcgt
     1801 atccccacac tttatttagt aaaacgtcac gtgctaatac cacgttcacg ttttccatta
     1861 aagtaagcag caactcgtat tctctttttg ttagttcaat tacttcattt ccgcgacgta
     1921 cgacacggtt ttctttttcg atagtcaaat cgcgataagt aatagtagtt tgtttagcca
     1981 catttttatc gccttcaata tcgattcgac gcaacaatgc acgaagacgt gctaataatt
     2041 cttcgatagc aaatggtttg acgatgtagt catctgctcc gtggtcaagt ccagatacac
     2101 gatcgatcac agaatctctt gcagtcatca taatgattgg tgtatttttg acttggcgta
     2161 cacgtctgca gacttctaaa ccgttcaatt ctgggagcat cagatccaac aatatggcat
     2221 cccactcatt atttaatgcc gcatctaaac cagttcggcc gttgtagtgg acttctgcgt
     2281 tatacccttc atgcttcaac tctagctcga caaatcgtgc caagttcttt tcatcttcaa
     2341 tgattagaat attgctcatt tattatttta tcctttctat tctactggct ttttaaaaac
     2401 tctatcatta taccgataat atgcttaaaa atctatagtc attttatcat ggatagcaat
     2461 tttaaaaaag agctgaagtc tactcataac aatcgacttc agctctttcg tcctcttttt
     2521 attttctcat ttcctaacag ccgaaagact attattcttc atggtaccat gagtaatgga
     2581 agattccttc tttgtcgaca cgctcatagg tatgtgcgcc aaagtaatcg cgttgggctt
     2641 ggattatatt tgcaggcaga cgttctgctc tgtatgaatc gaagtaagcg atggctgatg
     2701 agaaagtcgg tacaggtacg ccagcttgga cagccaaggc aacgacgtct ctgactgatt
     2761 gctgatattt tttcgtgatt tcaacgaagt aatcatctag caataaattt tcgatctcag
     2821 gatttttgtc ataggcatcc gtaatttttt gcaagaatcg agctcggatg atacatccag
     2881 cgcgccagat ttttgcgatt tcaccaaatg gcaagtccca atcaaattct tttgaagcga
     2941 cacgtaattg agcaaatcct tgggcataac tcatgatttt actgaaatac aacgcttctc
     3001 ggattttttc gaccaattct tttttgtctc ccgtgaactc aaaatcgttc gtacgagaca
     3061 aaatcttgct tgcttgtacg cgttcttctt tataagcaga aatatagcgg gcaaatacag
     3121 attcagtgat caatggaagt ggcacaccta agtcaagcgc gctttggctt gtccatttac
     3181 ctgttccttt attcccagct gcatcaagga tcacatctac tactggttta cctgttcctt
     3241 catcgtcttt acgtgtcaga atgtcagcag tgatttctat caaatagctg tctaattcgc
     3301 cttgattcca ttctttaaag atatcagcca tttcttctac tgaaaggttt aaaatattct
     3361 tcattaagtc ataagattca gcaatcagtt gcatatctcc atactcaata ccgttgtgta
     3421 ccattttcac ataatgtccg gctccatttg ggccaatata tgtgacacat ggtgctccgt
     3481 cttctgcttt tgcagaaatt tgctctaaga ttggtgcaac tagcgcatag gcttcttttt
     3541 gtccaccagg catgattgaa ggtcctttta aagctccttc ttcaccaccg gaaacgcctg
     3601 taccgataaa gttgatgcca gagttggcta actcttcatt acgacgcatc gtatctttaa
     3661 agaatgtatt tcctccgtca atcaaaatat cgcctttatc aaggtgagga agtaactctt
     3721 ggattgttgc atctgttgcc ggtcctgctt tgaccattag caagatccga cgtggtttct
     3781 cgatagattc tacaaattct tcgattgtat atgttgcttt gaaattttta tctgggtgtt
     3841 cttctactac agcggttgtt ttagcccctg tacgattgaa taaagccact gaatagccgc
     3901 ggctttctat gttcaaggct agatttttac ccataacggc cataccgaca acgccgattt
     3961 gttgttttga catatgaact cctcctacat acgggatttc tccctaatct cttacattat
     4021 agcttaacac ggctacattg caaactaaag aacaacatat tt
//
LOCUS       DAYYBG010000153         4030 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000153 DAYYBG010000000
VERSION     DAYYBG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.158"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..392)
                     /locus_tag="ACHAUN_002757"
     CDS             complement(243..392)
                     /locus_tag="ACHAUN_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461389.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            854..1066
                     /locus_tag="ACHAUN_002758"
     CDS             854..1066
                     /locus_tag="ACHAUN_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461390.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..3143)
                     /locus_tag="ACHAUN_002759"
     CDS             complement(1344..3143)
                     /locus_tag="ACHAUN_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8461391.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3267..3863)
                     /locus_tag="ACHAUN_002760"
     CDS             complement(3267..3863)
                     /locus_tag="ACHAUN_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8461392.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1181 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatct cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc
     2821 aaagagattg gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg
     2881 aaataaacta acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga
     2941 tgttgagaaa aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt
     3001 aaaataaaac gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc
     3061 atcagcaaga catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct
     3121 aagctacgtt tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa
     3181 tgaaaaagga aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat
     3241 caatgactaa ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca
     3301 taaatcaagg ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt
     3361 gtatatcgtt tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga
     3421 gcaactggaa gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc
     3481 cagccaatat atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca
     3541 taacgacgcc ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag
     3601 ataaatccag cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga
     3661 atgattggaa taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg
     3721 atggaatcac ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata
     3781 aaataaatcg tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt
     3841 tttcgagagg cagcaatatt cattattcat caactcctta gaggttcttt gttaactata
     3901 ctttactaga aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt
     3961 gtaagttcat cataagtata aaagattgat ctcaaaaaaa catccaacta aagacttatt
     4021 taataacttg
//
LOCUS       DAYYBG010000154         3931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000154 DAYYBG010000000
VERSION     DAYYBG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.159"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(579..1136)
                     /locus_tag="ACHAUN_002761"
     CDS             complement(579..1136)
                     /locus_tag="ACHAUN_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461393.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1315..1926)
                     /locus_tag="ACHAUN_002762"
     CDS             complement(1315..1926)
                     /locus_tag="ACHAUN_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461394.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2039..2296)
                     /locus_tag="ACHAUN_002763"
     CDS             complement(2039..2296)
                     /locus_tag="ACHAUN_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461395.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2893..3171)
                     /locus_tag="ACHAUN_002764"
     CDS             complement(2893..3171)
                     /locus_tag="ACHAUN_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461396.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3205..3456)
                     /locus_tag="ACHAUN_002765"
     CDS             complement(3205..3456)
                     /locus_tag="ACHAUN_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461397.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          803 c          521 g         1412 t
ORIGIN      
        1 gcctccttat tccgaataaa tggtgtccaa aagcttgcat ctgccagatt atctcatttc
       61 ttatgacaag ctaagcaaat tatctccacc aaataattta ttcatatttt tgacttgcgc
      121 atactaacta attcttaaat tttcatacaa aatttctcaa tttcccttaa cgagtaatat
      181 ttatgtacgg tgattctcac tagtatccca atcacttttt aagttatccc gatttcaatt
      241 ctacttcaaa aatagttgct tttatgtatg ctgttagttg attagatggt catttgttga
      301 gctaggtcgt ttattttaaa caagttgttt ctttagctta tacctatctc gtctaccaaa
      361 cgcaagatgg caaacacaaa tgcatacgca tctgtatttg ccactttgat tcgtataaag
      421 ctaacaacta ttaactcctc tcagctaata gttgctctct tttttattca gattcatttt
      481 tgctgatcac agcctagtat ttcaatggtt ttgaacacta aaccaaattg ttccattaca
      541 acattcactg gctgatgaaa tggattagta gatttcgatt attcaaagcg attttctatt
      601 ggtggatcgt gttcccaact aataagacgg attccaaacc tatcgtacag atcttctaaa
      661 ccacttataa attttgcacc tatttcaggt gttttattgc gtggatattc tattgttacg
      721 ccattaaatg gataccaatt aggattaggc tctctttttt tctgtacatc tcccatagaa
      781 tagttagcac tttctgggac ataatatatt cttcgaccat cctctaattc atctaataag
      841 atatttcgtg atcgtctatc acttgctaaa tgtagacgcc acgtgcgata ccatgccatg
      901 ctacctgcat ctgcagaaaa tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc
      961 ctttccccat ctacttcata ttctaatcta aaatcaaact cccctcgggt aatttctggt
     1021 cttggtggct taggacccaa tattattcta cttaaaaccg cattcgttcc cagatgaaac
     1081 acagctataa caacaataat gattaatatt ttgtttctat ttgataaatt tctcattatt
     1141 cgcacatcac ctctacattc tcacatatta gttcgtaata ctcctttaat atcaaaatat
     1201 ctactggtcg taataatatt cgctatgaat tcctactact cgtataagaa aggccaaatc
     1261 atcagctttt acttgctgat gatttggctt gtttctattt atcgtaattg ttttctaatc
     1321 accaaaattg ttaacaatcg gttcagatat ttcccagttg attaaggtaa ttccgtattg
     1381 tgtcaaaact tcttgcgctt ctccgaaatt aagtacagtt cttatgtatc cactttctcc
     1441 taatataaag tctacctctc ttttaaaacc ttcctcgaga tttctaaggg ctatatgtgg
     1501 ttttaatgac attgattgta cattttcccc catataatat tgcgcacttc ctcgactaaa
     1561 atagattcct agatcgtccc caaaaggtag atcttctcta ccactagcta atctacttct
     1621 ccatcttcgt tctcttttca tagaacctgc gctgaactca cacccgttaa actcagctat
     1681 gacagtatct tcaattatgt taatctgtcc atctagttcg tattcaatcc taaatggaaa
     1741 ttctccatag gtgatttccg gttcgggtat tttaggtgag attaatgcaa agaaaggaaa
     1801 tattaagctt ggcaacatca aaaaaactcc tcctatgatc acaattcccg atgtaatcca
     1861 aaatataatt tgtacaattc tcattgatcc attgtattcc attttattcc ccctattact
     1921 attcatatcc gcctatctat atcgatcttc caatgcagag ataattgctt ccataccttg
     1981 attttttact gagttaccta tgcgagatat cattctccac taaggtgcat catattgtct
     2041 atctaacatg attaattctc caataggtgt atactcctcc aaaaacaaca tttaatgcat
     2101 ctccctcgac aacataagct accatgtttg ctgtatatgt gtttgagtct aattcataag
     2161 cagataaatt tactgtcgct ggattaaata taatcgcatt cgtatttata gctactgaat
     2221 ttgctgcttc agcaccacct tttgaatgtc cgatcattgt gatttcaata gtaggattta
     2281 cgtggacagc cctcaccgtt taccctgacc attatagaaa tcgcttactt ttttgaacta
     2341 cgcaacaaag gatacaatgc tcctaacttc actgtctcca tttttgtcca ctaaggaata
     2401 taaaaaatag gcattaattc atttaattcg gtcacaacac gttacctttt aaatcattat
     2461 actgtatctc atacattatt atcaataaac tattataaaa atgcaaaaaa tgctttatca
     2521 atctattttg taattggtca taatatcatt gaaatcatac cctgtatcga tttttcctat
     2581 gaatctcgtc tatttgtgat tgacgaaaaa gaggcaagac agacactgga atggtaattt
     2641 tacccactat tttgctccaa ctatttgtaa tcgtacccac ttttgaaata ccgagatgat
     2701 cattttaatt gatatgctga ttcccaagca aaaccccaag aaattaaatg ctctatcgca
     2761 tctagcttct tgggttttgc ttatataaat tccaacattt aaatttttta tgctgttcgg
     2821 tctcagaaat gccgtttttg cttatatcac aatatcaaga tgtcaaatta ttcgtgttgc
     2881 cgatgacttt gattaatttt tttcaaattg tttattaaga ctaatgctgt ctttttgcag
     2941 ataccaatgt tcgaactttt taacattatc atatgatcct gcaagtttta acttcttacc
     3001 attagatgtt atcaaagtta taattaacct tgcctttttc ccattaggat cttttatttt
     3061 atgaattttc tgcaggtaat ctctcctaat ggcagctatt tttacctcat cagtgctgat
     3121 aaaccattga tcatccacat acatactgcc tgttatgttg tgtttttcca tttcttccga
     3181 gaacgaaaag tttaaggctc gttcttactt ccaaagttga atgagtccat ggattggact
     3241 tagtgtgaga attgagaaca acaataagaa tccatatcct cccgcaaaca tccctaaggc
     3301 tatactgtct ctccatccat aagtaggatt agaagagaaa agagtcaccg tttgtaataa
     3361 tatgaaaagg aataatccag cacaaacacc aaagataaca actccaaaca ttgaattttt
     3421 atacaatgac catttattta taggcttttc tttcatttta cttcccccta ctgttctgtt
     3481 cacacggaat gaattcaaag ttcataagcc ctcctctttg aacatatgaa tacgacttgc
     3541 taactatact ttaaaaaagc ttgttttaag cggttatagg cgagttgtaa tctctattca
     3601 tctcattagc aaataacttg ccgaaaagtc tactgaaatt aaagcagata gtggaagaat
     3661 tatttagcca ggatagacac tatttgtgaa acacatgtct ttacttttga aattctatga
     3721 tgaaaagcct tggaatacag tagctcttaa ttggtaatac tttttccatt ataaataacc
     3781 tcttttcatt ttatatttat cccctctaaa aataatatct aatctaatat tttaaactta
     3841 tatttccctc gctgtgctaa tagagcatta ctaaataaat agaaactaga aaaaagcatt
     3901 acgcctgcca gaataaacca attatttgta t
//
LOCUS       DAYYBG010000155         3863 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000155 DAYYBG010000000
VERSION     DAYYBG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.160"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACHAUN_002766"
     CDS             complement(245..466)
                     /locus_tag="ACHAUN_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461398.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACHAUN_002767"
     CDS             complement(564..809)
                     /locus_tag="ACHAUN_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461399.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACHAUN_002768"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACHAUN_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACHAUN_002769"
     CDS             complement(2033..3034)
                     /locus_tag="ACHAUN_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8461400.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACHAUN_002770"
     CDS             complement(3193..3459)
                     /locus_tag="ACHAUN_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8461401.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACHAUN_002771"
     CDS             complement(3449..3805)
                     /locus_tag="ACHAUN_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8461402.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAYYBG010000156         3826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000156 DAYYBG010000000
VERSION     DAYYBG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.161"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1238)
                     /locus_tag="ACHAUN_002772"
     CDS             complement(<1..1238)
                     /locus_tag="ACHAUN_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461403.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSF"
     gene            complement(1336..1611)
                     /locus_tag="ACHAUN_002773"
     CDS             complement(1336..1611)
                     /locus_tag="ACHAUN_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461404.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            1843..2577
                     /locus_tag="ACHAUN_002774"
     CDS             1843..2577
                     /locus_tag="ACHAUN_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8461405.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            2616..3758
                     /locus_tag="ACHAUN_002775"
     CDS             2616..3758
                     /locus_tag="ACHAUN_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8461406.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         1381 a          659 c          565 g         1221 t
ORIGIN      
        1 taaaaactca tttttttagc taattgttcc cccgacaaaa tacaagtcac actttcctta
       61 ctatcatcaa aaaaattaac atcagaaagc aagacatcat agacacgtcg catttcctct
      121 agataattcc ttattgtcct gaaaagtttg tctttcattg attcaagagt gtcaaaaagt
      181 acttttgatt cctctttcaa ttttcgaaaa tgtgtaggat acaagtctag gttctctaat
      241 agccaattaa atgtttctac ccgactgtct ttctttaaaa ttagcatcac aactccaata
      301 aaattttcac tgatatcaca attactggaa tgacaataaa aaatggaatg cccagtttta
      361 ctggtaaaaa tactagcaga gggattatga tcgttgtgaa acagacatct taagttttta
      421 cttgacttaa gtaatttatt catatcaatg gttaacaatc tttcataaat ctcattaaaa
      481 gacatgtcgg aggaatcaaa caggcagtct ttaaaccact gtttcatttc ttctttgtcg
      541 tcatttttta tcaacttaac aaaatcggtt tcattgacta gttcatactc tctccctgaa
      601 tcactttgac aatttttaga acatgtcatg gtaaaatcaa tcggaagctc agtaatatct
      661 aacatgttgt cttcattaat tattatcact gcatctgact tatatcctcc aaaaaacatt
      721 ctattcgaag catttacatt gtcatcacac ccggggatgt tctttttcaa gtattcgtaa
      781 acagcaacca ttttaccata gtcagtaatt gcttctttta atagaaagac aatcttgtat
      841 ttttctagat tttcagagta ccgaatactt ttttgtatcg caaacgcaga attatgaact
      901 aaattgatag ataaaacctt ttctatgctt aagtatctac tatcatcttt actcaaatct
      961 actaccttcc ccttctcatc tttaacagtg ttatcgacat ccagaataat tactctggtt
     1021 tccacaaaac tctccttgtc attgccttta ttctttgtct tgcttggaat aaatgattgt
     1081 gatgaattta gtatctctac aaactcatct cttgtaactt ctctatagtt atctaaacac
     1141 ctatctctta catcagtttt ttcttttttt gtaactggct tctctaataa tttttcttta
     1201 tctacataaa ttggaatatt aatcattgat gtttccattt gtgtttcctc cttataaaat
     1261 tctgttaaag actaaaacat acagataata aaaacaaact ctttattact atatgtttta
     1321 gtcttatttg tttactcaac ctttataccg ttccatgaaa ctatcaatat cagatttttt
     1381 aaatctaatg accgtatcaa tttttataac aggtaacccg tattgagaaa catattttct
     1441 aatcgttcct actgatacac ctagatattc acttgcctgc tgatagtcca tatactctag
     1501 agtgactcca gaaataatgt cttctagcat aggctttagc actgtaatta tctctttcac
     1561 ttgttgttca acttgtatag aaacaatgct atcaatgatt tgtagagaca tatttccccc
     1621 tcctttcgtg attgcttgaa caaattttct aagaattatc ttcgtatttt ttgctcaaaa
     1681 taaaagtatt ttgttattaa ccgattaatc aaacaaaaaa tgattaccag ttcatcgaca
     1741 tacacataat acctcgataa aaaagaggtg tcaatatctt tgtaaaattt atttacaaca
     1801 cccctttttc gaggtatact aattttagga ggtgtggtca atatgataaa acgtccacca
     1861 ataaattatt tagaacgaaa aaagatactc ggaacaaaaa taaaagctat aagaaagagt
     1921 aaaaaattaa cacaaccagc atttggtctt atgattaaca acggtcaatt aattgacaaa
     1981 aaaactatct acgaatggga gaagggaacc tatttaccaa taccagagcg attatcccga
     2041 attgctgatt tgggaaatat gagtatagag gagttagttt gtggcaatgt agaagaatat
     2101 attttgggaa ttattcttta tagagatagc attgtactag atggtataac atttccagat
     2161 aaaaacctgt tccagcatct tcgtcaacag ttcccaccag tacactctaa tttagataca
     2221 tggctggata gatattccaa attagaacca gagatgcaag aatttattgc taacaagact
     2281 tgtaataaag ttaaaaatga aaaaattagt cttttcaata tcttaaaaat agaagaatta
     2341 ttcatcaatg ctattgtaga agaatttgac aataacattc ttttcctcac tagcagtata
     2401 gaagaactac tagaaagaat ggtagatgaa tggctaccta ttcaattgaa agacatgtct
     2461 tatcccgaag aagctgtgag agaaattact gataatataa acaagctaga acaaacgatt
     2521 tcatcaattg ggaaaaaata cactaagaaa aaaatgaagg gaggtgatac catctagtgg
     2581 taagtactcc ttgtaaacta agaggagatg atttcatggc aaaaatatca aagtacaaaa
     2641 agaaaaacgg tcaagtggcg tggatgtata aaggacacat tgccaccgat ccacgaactg
     2701 gtgaaaaaat aaatactacg agaagaggtt ttagtagcaa acaagaagct caaaaagatt
     2761 atgacgagta cagacatcaa atattatacg gtgttaaaaa gaaagcacct gatatgacat
     2821 ttgaagacct gtataatgaa tggataactc atcagcgtac cagtgttaaa gcttcaacaa
     2881 tcgcaatctc agtaagatac gcaaataatc aaatacttcc agcatttggt aagcttaaac
     2941 tatcgaatat ctctgtccca tattgtcaaa aagtggtaga tgaatggcac agtaaatatg
     3001 aaagttacga ttatatgaga aaacagactg cacaaatttt acgctacggg gttgcaatgc
     3061 aatatattga caataatcct atggaaaaaa cacttcttcc acgaaaaaaa gagtatgaga
     3121 aaaatcgtaa attttactca aaagaggagc ttaataattt actggatgct tttaaagatt
     3181 ttggtaacat gaaacaatac gccttcttca gattattggc atacacaggt atgagaaaga
     3241 gtgaagtact cgctttacag tggaaagaca tagacacgtt taataaagaa ctacatgtca
     3301 ataaaactct tgctgtagat gaatttggta aagttataat tcaatctccg aaaacaagag
     3361 cttctagacg agttatctct ttagatactg agactctttc tatcctcaac aactggaaat
     3421 tacagcaaaa agaagagtat ttaaaacttg gatacaatac tagctctaaa gagcaacatg
     3481 tttttacaac agttaaaaac actctataca ttccaaacac tgttaatgat tggttaagat
     3541 atattttgaa aaaatataac ttgcctcgta ttacacctca tgggtttaga catacacatg
     3601 ctagtttatt attagaagca ggagaatctg tcaaggtagt tcaacaacga ttagggcatg
     3661 aaaatagtaa agtgacatta gacatctatg cacatattac aaataatgca cctaagaaaa
     3721 caggtcaaga ctttgctgat atgatggctc atcagtaaaa gagctggtca aaatctggtc
     3781 atttctagta tacaggcaaa acaaaaaagc tagaaccctt atatat
//
LOCUS       DAYYBG010000157         3752 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000157 DAYYBG010000000
VERSION     DAYYBG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.162"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHAUN_002776"
     CDS             <1..1087
                     /locus_tag="ACHAUN_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8461407.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACHAUN_002777"
     CDS             complement(1305..2447)
                     /locus_tag="ACHAUN_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8461408.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACHAUN_002778"
     CDS             complement(2452..2637)
                     /locus_tag="ACHAUN_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8461409.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACHAUN_002779"
     CDS             complement(2991..3236)
                     /locus_tag="ACHAUN_002779"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461410.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACHAUN_002780"
     CDS             complement(3230..3631)
                     /locus_tag="ACHAUN_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8461411.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1238 a          679 c          567 g         1268 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc ta
//
LOCUS       DAYYBG010000158         3664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000158 DAYYBG010000000
VERSION     DAYYBG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.163"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..277
                     /locus_tag="ACHAUN_002781"
     CDS             <1..277
                     /locus_tag="ACHAUN_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFM8461412.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="ACHAUN_002782"
     CDS             420..641
                     /locus_tag="ACHAUN_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461413.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="ACHAUN_002783"
     CDS             657..1499
                     /locus_tag="ACHAUN_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461414.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="ACHAUN_002784"
     CDS             1562..3364
                     /locus_tag="ACHAUN_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFM8461415.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="ACHAUN_002785"
     CDS             complement(3423..>3664)
                     /locus_tag="ACHAUN_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8461416.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DAYYBG010000159         3602 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000159 DAYYBG010000000
VERSION     DAYYBG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.164"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACHAUN_002786"
     CDS             <1..186
                     /locus_tag="ACHAUN_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8461417.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACHAUN_002787"
     CDS             329..553
                     /locus_tag="ACHAUN_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461418.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACHAUN_002788"
     CDS             568..1437
                     /locus_tag="ACHAUN_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8461419.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACHAUN_002789"
     CDS             1418..2152
                     /locus_tag="ACHAUN_002789"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8461420.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACHAUN_002790"
     CDS             2185..3093
                     /locus_tag="ACHAUN_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8461421.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACHAUN_002791"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACHAUN_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAYYBG010000160         3542 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000160 DAYYBG010000000
VERSION     DAYYBG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.165"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..970)
                     /locus_tag="ACHAUN_002792"
     CDS             complement(266..970)
                     /locus_tag="ACHAUN_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461422.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1157..1402)
                     /locus_tag="ACHAUN_002793"
     CDS             complement(1157..1402)
                     /locus_tag="ACHAUN_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461423.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1419..2270)
                     /locus_tag="ACHAUN_002794"
     CDS             complement(1419..2270)
                     /locus_tag="ACHAUN_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8461424.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2436..3440
                     /locus_tag="ACHAUN_002795"
     CDS             2436..3440
                     /locus_tag="ACHAUN_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8461425.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1099 a          603 c          688 g         1152 t
ORIGIN      
        1 ttttttctct atcagaaaat atgtttatta ttagaatttt ttatagttaa ggttaggcat
       61 cttaagaaat caggcaactc caagatatct tcaggtagaa gagaagtcgc ctaacattgt
      121 aggatcatag gtgatttgtt ggacaggtca agctgatatt tgagcctgat gttagaagga
      181 atttcttttt acttgctgta gaaattatta tatgactaaa aaggctggga ttttaaatcc
      241 cagcctttta tgacaaatag aatttttatc gatcagtaat cgctgtcgct gcaaattgtt
      301 cgatcaaagg aatcaaagtc aatttgactt tgtatttccg ttcgagtgct ttttcgataa
      361 cgaactcatt ttcagtagtc atagaaatag ttgctaattt acgaggttca tcagcatttt
      421 tttcattaat aactacttca tgttcatttt gttctgtgta aaatttagga tactcagcaa
      481 cggcttttga aaaatcagca aaacttaaag cggcattcgt atcagttgag cgtgtatttt
      541 cagttgtgtc cgcttttggt ttttccgttt tttcttctgg cacactgact gtttcttctg
      601 taactggtgt cgcaacagct tgcgcagttg gttcatcgtt tttcaatcgt tgatcattcc
      661 agtaagacac gatttcaata agaagattgt cacggaatcg ttcgaaaatc ccgttttctc
      721 ctgaacgaat tctctttaat acagtattga agttgtcatc gattggcacg ttgatccgtg
      781 tatcttcatt tgttttccca gctaatccga tcgttaagat ttgacggttt ttgggatcaa
      841 gcatgacttg taacggcaaa ccataacgtc gcaagctctc ttcttctaga gaacggaata
      901 attgtcctct tacaccatcc aatgaaggac ggaattgatt taataatttt tgtttctgtt
      961 ttttcttcat atgttctctc cttcgctcat accctttcga ttataagaaa aaaacagcca
     1021 aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa attacaacag aaaaatttgc
     1081 gtattttccc gttttttgaa agaaaaataa aaaatggaac ggattatgta tatatacata
     1141 atccgttcca ttctttctat tttttcggta tcaaggcaaa aacaggtagt tcaggtgaat
     1201 aaatgacttc atgttcgtgc cagggtttga ccattagcga atgatcgaac cgttttttca
     1261 gttcttcgat gatttgatca tgtgtccagt ttcttgcagg aaaatgttgg attttctctg
     1321 catcgatcaa aaagacaaaa tcggaatgaa aagtctgggt aacttcttcc gagagagaat
     1381 tcaagcgttc gattggatcc ataaaatcac ctttcgactt aaaattgggt agtatctggg
     1441 tcttttgctt tgccgactac accatctaac tgatcgattt gtttcatttc ttcttctgtt
     1501 aattcaaaat caaataattc agtattttct ttgattctgc ttggtgtcac tgacttagga
     1561 agaggaagga attcgtgttg cagcgaccaa cgcaaagcaa cttgtgcaat cgtcttgtca
     1621 tgtgcttcag cgatttgttt catttcaggc acatcgaaga ttttaccagt gcctaatgga
     1681 ctatatgctt cgaggatcat atcatgtttt tttgcatact caactacagt tgtctgtaat
     1741 tcgcctggtg caaggaaaac ttgattgacc atcggcataa tttttgctgt ttttgctagt
     1801 gtatctagat ggtggggtaa gaaattgctc acaccgattg ctttgatttt acctgcttca
     1861 tacagttctt caaatgctcg ccatgtatct gcatttgctt gttcccaatt atcacgaaaa
     1921 gcaacagggt ttggccagtg gatcaaaaac agatctaaat aatctgtttg gagcttcttc
     1981 aatgattctt caaaagaaga catcacaagt tcatagctgt ggtttgcatt ccataatttt
     2041 gttgtcaaga atatctcttc tctaggaatt ccactttcct taatcgcttg gccgacgctt
     2101 tcttcattct tgtatccttg ggcagtatcg atatggcgat aacctgcagc cagcgcttct
     2161 ttaacagagg aaacagcgac atccccatca ggtgtttgcc atgtgccgaa tcctactacc
     2221 gggatttcgt aaccatttga tagacgataa gtgcttgtta atgaattcat actttcagct
     2281 ccttttttat tcgttcaact ataagtgtag cgcatttttc gaaaaaaaga atccattttg
     2341 tttctactct tgaaattttt gtttaaaaga aaatgatttt gattctggta tttttgattt
     2401 tttttccgta aactaagaca gaagggaatg agaaaatgga tatctattta aaaaaagcaa
     2461 ttctgcatat tattgacaga gaaactggtt ctccagtctt ttcacaaaaa gagctggatc
     2521 tgacaaaaga atatattcgt gattttcttc aaaagaagat ccaaaaaatc tcttctgctc
     2581 aaacaaaaac tggacaacta gcagaagaaa gcatgatcgt tgaatcactt aaaatcgctc
     2641 aagaaaattt tgtggaagca agtgaagcaa tcgttcagcg gtggttcgat atctaccaag
     2701 aaagtgaaga agcacctagt gcagatgtat ttgttgtctt gtatgaatta gatacagtga
     2761 tgtacttatc tttattgaaa gtcaattatc gtgaggctta cactcacttt gttgaagcag
     2821 acgaagcagg gatcgacaac cgcttgattt tgaatcgagc aattctaggg gcgaaaacac
     2881 aaaaagcaga tgaagcattc gctgtgaatt tgactgattg gacctacgaa ttaattgaaa
     2941 aaaaatatga gttttctggc aaaaaagagc tttatttttc tggaagagtg atcgaaagtg
     3001 ttcctgcacc ttctttagaa gaaaatgtaa aagtgatcca aaaagtagcc aagcatcttg
     3061 gaaaaaaatt tgagacagaa gaatttgata tcatggctga tttgaaagaa gctgtctatg
     3121 atacgataga agaaaaaggt cggttggatc atgagatgat tgcggaaaaa gtcttcaagg
     3181 aaaacatcac ggcaaaaatg gcttttaaag aagaagtaca agagcaagga tttgtcccag
     3241 aagcacctcc tgtaaaagaa gtacaagaaa tttctgagaa aaaatttgga aaacaaaaat
     3301 tcaagctatc aaacgggatc gaactgatcg taccagtaga tgtatatcgc aacccagatt
     3361 tgattgaatt tgtgaataat ccggatggta cgatatccgt gatgatcaaa aatgtggatg
     3421 aagtattgaa tcgtttataa aaatgactag aatattaggt gttcaagtac cagtgatcag
     3481 ttaaatgaat tgtgtcaaga gaaggatcgg aaaagacgaa tataacaaaa aaagagcgtg
     3541 ag
//
LOCUS       DAYYBG010000161         3394 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000161 DAYYBG010000000
VERSION     DAYYBG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.167"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACHAUN_002796"
     CDS             complement(<1..2177)
                     /locus_tag="ACHAUN_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8461426.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACHAUN_002797"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACHAUN_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          954 a          747 c          538 g         1155 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaa
//
LOCUS       DAYYBG010000162         3200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000162 DAYYBG010000000
VERSION     DAYYBG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.169"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHAUN_002798"
     CDS             301..585
                     /locus_tag="ACHAUN_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461427.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHAUN_002799"
     CDS             588..1004
                     /locus_tag="ACHAUN_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461428.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHAUN_002800"
     CDS             complement(1141..1860)
                     /locus_tag="ACHAUN_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8461429.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACHAUN_002801"
     CDS             2522..2608
                     /locus_tag="ACHAUN_002801"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8461430.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..>3200)
                     /locus_tag="ACHAUN_002802"
     CDS             complement(2911..>3200)
                     /locus_tag="ACHAUN_002802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461431.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAYYBG010000163         3200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000163 DAYYBG010000000
VERSION     DAYYBG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.170"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHAUN_002803"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHAUN_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHAUN_002804"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHAUN_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="ACHAUN_002805"
     CDS             2092..3132
                     /locus_tag="ACHAUN_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8461432.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT         1185 a          447 c          461 g         1107 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta
//
LOCUS       DAYYBG010000164         3188 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000164 DAYYBG010000000
VERSION     DAYYBG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.171"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..825
                     /gene="fabI"
                     /locus_tag="ACHAUN_002806"
     CDS             70..825
                     /gene="fabI"
                     /locus_tag="ACHAUN_002806"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8461433.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(935..1987)
                     /locus_tag="ACHAUN_002807"
     CDS             complement(935..1987)
                     /locus_tag="ACHAUN_002807"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8461434.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2113..3033
                     /locus_tag="ACHAUN_002808"
     CDS             2113..3033
                     /locus_tag="ACHAUN_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8461435.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          936 a          643 c          605 g         1004 t
ORIGIN      
        1 ttattttagc tattattctc tattttttta cgactatcaa atttttatca gaaacaaagg
       61 agagtcatta tgtcatttct agaaggaaaa aaaatcgtca ttatgggcgt cgctaacaaa
      121 aaaagcattg cttggggttg tgctgaagca atgttgaaac aaggggcaga aattatctat
      181 acttaccaaa atgaacgaat gaaaaaatca ttagtcaaat tggtcggcga agaagcattc
      241 actgtagaat gtgatgtttc tagcgacgaa agcatcgaaa aagcaatgaa tgccatcggt
      301 gaatatgctg gggagatcca cggccttgtc catgcagttg cttacgccaa caaagaagaa
      361 ttgtccggaa atgtgtctga tatttccaga gacggctatc aattagctca agatatcagc
      421 agctactctt taattgctgt atcaaaatac gcacaaccgt ttcttgcacc aaacagcggg
      481 atcgttagca tgacttatct aggttcagaa cgcgcaatcc cgaattataa catgatgggt
      541 attgcgaaag cttctcttga agctgctatc cgttatttgg cagctgagtt ctcgcctaaa
      601 ggaattcggg tcaatggaat ctcagctggg gcaatcaaaa cattagctgt cacaggtgtt
      661 aaagactatc aaaaactgat ccagttatct gaatctcgta cgcctgatca gcaaggtgtg
      721 acgatcgaag aagtcggtaa tgcttgtgct ttcttaatca gcccactttc cagcggaatc
      781 atcggtgata ttatctacgt tgacaaaggt gtccatcttt cataaacttt tgttgcattc
      841 tttaaagtaa aacatataaa aatcaggaaa aaacttttta aagtctcttc ctgatttttt
      901 ctttttacgt ttttttcaac tcattacttt gaaattattc cacgacttca atccagcctt
      961 ctggtgcttc tatgtctcca aattgaatac ctgtcaattc atcatacaac cgttttgtta
     1021 ctggtcctac ttccgtttca ctataaaata catgaagatt gttattgtga taaatcccac
     1081 cgattggaga aataacagcc gctgtaccac atgcaccagc ttctgcaaat tgatctagat
     1141 catcaatgaa aatatctcct tcaattgctt gcagtcctag tcgttcttca gctaactgca
     1201 acaaggaata tttcgtgata cttggtaaga tagagggcga ttgcggagtg atgaattttc
     1261 cgtcttttgt aattccaaag aaatttgctg cacctacttc ttcgattttc gtatgtgtgg
     1321 ctggatcaag ataaacacaa tctgagtaat ccagttcttt tgcttctgtt cctggaagta
     1381 aactcgctgc gtaatttccg ccgacttttg ctgcaccagt ccccttagga gcagcacgat
     1441 cgtaatcaga tacaataaaa ttcgttggtt ttagtccacc tttgaaatac gctcctactg
     1501 gcgtacagaa gacgacaaat aaatattctg gagctggatg gacaccaatg ttttcaccga
     1561 ccccaatcag taaagggcgc aagtataatg tagcgcctgt tccatagggc ggcacaaaat
     1621 cttcatttgc ttttaccact tgcttgacag catcaacgaa catcttttca gggaccattg
     1681 gcatcagcaa tcgtctagca ctttgattca tccgtcgact attttgttca ggtcgaaaga
     1741 gattgacctt gccgtctttt cgacgataag cttttagacc ttcaaaacat tgctgaccat
     1801 aatgaagcgc tggagagcct tcatgaatcg tcaggtaatt atcttctgtc aattctcctt
     1861 cttcccattg tccgtctttc catttggcaa taaaacgcca aggagttttg atataagaaa
     1921 aacctagatt gttccaatcg atttgcatat acttaccacc ttcgttattt tctccacatt
     1981 ttatcattat ccatattatt tgtcatttta ttttcagctt ttttccttca ttatagttat
     2041 ttttgctatt tccttttttg aaaacttctt ggtacaatac agaaagaatc aaattttaga
     2101 aaagaggatt ttatgttttc attattagca actgccggta cgacagactc ttctggcgag
     2161 acattgacag atcaagctgt cagcaaagta aatgcgtttc agcggttttg ggacgggatt
     2221 gattgggacg ccattttggc tgcctttatc caaaaaagtt tatatctgat ttttttgatc
     2281 gtcctttttt ggattcttca ccgtgtggga aaatatctga ttgataagtc ttttaatcag
     2341 tattctaaaa aagccattct aaacgagagc cgcttaaaga ctttacatag tcttttaaat
     2401 actatttttc attataccct tggtttcttt tttatctatg caattttatc tgctatcggt
     2461 gtgccgatcg gatcactttt ggctggtgcc ggaattgcag gtgtggcaat cggtcttggt
     2521 gcccaagggt tcatgagtga tgttattacc ggatttttta ttatcatgga acaacagatg
     2581 gatgttgggg actacatcaa gctagcaaac ttaacgattg aaggaaccgt cgtatctgtt
     2641 ggtatccgta cccttcagct gaaagcaacg gatggtactg tccatttcat cccgaatcga
     2701 aacatcacca cgatcagcaa tctctctcgt gcaaatatgc aagtactttt agatattcgg
     2761 attgttcctg aagaaggata tgacaaaatc tatgagatca ttgatcgagt aaatcgtaca
     2821 ttagctgaaa aataccaaga agagctccaa actgaacctt ctatctttgg attagtggat
     2881 cttggaaaca gcaattttgc gattcgtacg gtctgctacg cattgaacgg aaaacaatat
     2941 gccttaaaag aagagttctt gagctcctat gtcaaagaat tgacagcttc tggtttcacg
     3001 attccgaaca gtccaattgc tgtcggaaaa taatttggac aaacgatgca acgaatctca
     3061 ctaaaaagag cgtgggacaa aagtaaagaa cacttttgtc ccacgctcta aacacgtata
     3121 aacggcggaa gcagaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa
     3181 gcaatttt
//
LOCUS       DAYYBG010000165         3065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000165 DAYYBG010000000
VERSION     DAYYBG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.172"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(495..779)
                     /locus_tag="ACHAUN_002809"
     CDS             complement(495..779)
                     /locus_tag="ACHAUN_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8461436.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(1298..1783)
                     /gene="vanZ-A"
                     /locus_tag="ACHAUN_002810"
     CDS             complement(1298..1783)
                     /gene="vanZ-A"
                     /locus_tag="ACHAUN_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFM8461437.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1936..2847)
                     /locus_tag="ACHAUN_002811"
     CDS             complement(1936..2847)
                     /locus_tag="ACHAUN_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFM8461438.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          925 a          566 c          434 g         1140 t
ORIGIN      
        1 ttttgtttct aagtagatat ttctcttttc taactctttc ttaaacgttt ctagtgtata
       61 gatatttgct aattttctta tctccaataa actatttttt atataagttt tacattcatc
      121 atgattcata caaactccac cttctataaa tgaatacaaa aaaagcaatc aaacgatttc
      181 cgattgattg cttaacaatt cttaaattca gtagcttaga tacttgaaaa ctctctgatt
      241 tccctatata atgatagtac ggttatatac cgtcttcaaa caaagttaat taaataactt
      301 cttacgaggg aagagttcat ctgactaact gataagcgtt ggtttggcaa tcttatcggg
      361 ctatgcattt ataaaatgtc gtcaaacatt ttataaatgt gtcatggctc ttttttcgtt
      421 tctattcagt tcgttgtttc gttatatcta gtataccgct tttaaaaaaa ataagcaacg
      481 atttcgtgca ttattcacac gaagtcattg cttttttctt cttccatttc taaatccaat
      541 gttacttgtt ctgattctgt ttctggttct ggttctgttg gctcatttgg gattaaatcc
      601 actactagcg ttgagttagt taactttgca atttgttcta gtgtttttat ggttggatct
      661 gattttcctg attctattcg tgaataattt gatctactca tttctaattc ttgggagaat
      721 ttttcttttg atttggtacc tctcatcttt tttaagcttt ctctaaatgc tagttccaag
      781 tttctccccc ctctttttgt gtaataaaat tcacgcttta tttaaattat agcttttaat
      841 atcttaataa acaagtgatt aatcacttgt gatttgtgat tattccaaaa gtaggtaatt
      901 tcaccaacct ttttcaaaag tttttatcga cgattattgg actggatcgt gtttcctaaa
      961 gcgattctaa gcgcatgttt aattttaaag tgggtaattc tctttaaaac gtcctacaaa
     1021 gattcaaaaa aagcatggct gctagtgggg gtaccgtcag gaaaatgcgg atttacaacg
     1081 ttaaggaaat ttattaaaga gaccaattcc tcagcactaa aattgataat tgggctttct
     1141 ttgttactat agtgttaggt aagcttttta ctttatcacc ggttttcccc tcacttcaca
     1201 cctacgggcg agtttcccca catcccgttt tccatctaat ccaattcatt caatatcttt
     1261 atgtgtatga aagatcagat tgcttgattt agacatctta cacgtaattt attcttaaat
     1321 gggtacggta aacgagcaat aatacgagca aaagtatacc tacaaaaata ataactctgt
     1381 ctaatttttt ttgattcata tgcttattgc ttaaaccata taatttcagt ccaagaaagc
     1441 ctccaacagt atttgtaatt acatctgtta tgtctgtcgc tccaatagcg aagataaatt
     1501 gaattatttc aaaagtaaga cttaaaacca gtacaaaagc aaacttaggt aaaaatccga
     1561 tttctttaaa attgacattc aaaagcaagc caaatggaat aaagattata acattatcta
     1621 tcatctctct gaaattccca gtagcagtaa atggagtcaa gttaagactt ctttgatgat
     1681 aattaaatac tgataaaata ttgtattgta atttgaataa cactaaccag attagtgtca
     1741 ctaaatataa agctagcaat cctctagata atatttttcc caatataatt tcctctaatt
     1801 cttttgttta agttgtatca ttagtatagc aatgtcctag ttaacgtggg ttttaaatta
     1861 aaaagctaac gttaccccaa aagtgctagt acaataatgc tgttcattaa tttcgttggt
     1921 ttcattccgc catccttacc tccttgaatt agtatgtgtt gatccgggaa acactgtcac
     1981 aattacaccg tctatattgt ttcctgatat ctcataacga agattagtcg gcacatgaat
     2041 ggtggtattt ttagtaacag gataataaaa gatctcatat ttttccccat ttacactaac
     2101 agaaatggtt ttttcttctt ttaggtaatc catatattcc tcgagaacga aattcttttc
     2161 tttcataatc gcactatgtg gtaaaccaac atagcgaata tgccatggtt catattgaat
     2221 tcctgttaac tctgttttgt cctctggata acgtaaaatg aacccgtatt tccaagcatt
     2281 ttcttctatc cactttcctt caggggctcg ttccattttc gtcaagcttg atcctacatc
     2341 tagtgataaa cctgaattat gctcactata acctgctggt aaggcatact cagcccccat
     2401 ttcttggtaa agcacacttt gctcatcaaa gtctcgatag ccactattaa taataaaatg
     2461 actaacgcca ccctttacag catcattgac catctctgaa aatttttgtg ctatttcttt
     2521 tgacatataa atattactat caagcaaccc gtatccattt attaattcgt catgtttaga
     2581 taaattcacg atatctgact tcacactttc ttggcgaaca ggatatttac tattgattaa
     2641 tagcagattt ccttgataaa cctgttcttc tgtaattgtt ttctcttggg tattttcaga
     2701 agtcccacta ttttctaaat gttctttggg attttgatca taattttgaa attcgacttt
     2761 ttcctgagaa aacagtgctt cattaacgta gtcataacct aagtatatta agaataacaa
     2821 taacaataaa aaaaacaact tcttcatttt cagtctcctc cttaactata tagttggaag
     2881 tatgaaatat ttctagaatc ttccccttaa acggcacaat ctcgtttgag attgtgccgt
     2941 tttgccccgt aaattacagt caattatttc gccctactgg atttactatt atcaccaatg
     3001 tagatgaatt ttcataagtg atacccatgc tgaatttaac gaggctgaac tgcctgttga
     3061 caatg
//
LOCUS       DAYYBG010000166         2979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000166 DAYYBG010000000
VERSION     DAYYBG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.173"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACHAUN_002812"
     CDS             <1..189
                     /locus_tag="ACHAUN_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8461439.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="ACHAUN_002813"
     CDS             complement(275..1369)
                     /locus_tag="ACHAUN_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8461440.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="ACHAUN_002814"
     CDS             1558..2319
                     /locus_tag="ACHAUN_002814"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8461441.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="ACHAUN_002815"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="ACHAUN_002815"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8461442.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          958 a          585 c          499 g          937 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatag
//
LOCUS       DAYYBG010000167         2977 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000167 DAYYBG010000000
VERSION     DAYYBG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.174"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            651..>2977
                     /gene="esp"
                     /locus_tag="ACHAUN_002816"
     CDS             651..>2977
                     /gene="esp"
                     /locus_tag="ACHAUN_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8461443.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1087 a          449 c          579 g          862 t
ORIGIN      
        1 aatagattat aagtatttga gtgaaagcaa ttaattattc atgtagagta ctagacactt
       61 taaatatgga caattcaatt ataaagaaaa agaatcaata atttaatgag gctgtgacaa
      121 atgtcatttt ttgtttcgct aaaaacgagt gaaaaaatgt tgataactcc aatcattcgt
      181 ttaagattct aggaatcatt caggaatgct gtgaaaccga tactttataa ttataaagaa
      241 ggtgatttca aattccgaag aaggaatttg aagtcacctt tttacttatg tcacagtctc
      301 attcagtaat gcgggataag gctccttatt ccgctatttg aatactctga agtttaatct
      361 aagaaagagt aatattttga aaacatgctt catcgcatag aaatgaactt aatttcagaa
      421 agaatacata catatctttt tatatgtttt cagctgctga atggttaaaa agcacaaata
      481 tccgaaaaaa acatgaagaa atgtactttt tttgttgtaa tgttcatgaa attctctaag
      541 taaattagtt aacataaggt tagtaagatt cctattctgt gaataatacg tatcagattt
      601 atcttttatt cttactagga aagtaattat taagggagtt gatttgaata atggttagca
      661 agaataataa gagagtattt ctagaaaaaa caaagaaacg agtattgaag tacagtatca
      721 aaaaattaag tgttggggtt gcatctgttt tgattggtgt tggtcttgtc ttgggaacaa
      781 ctgaactagt taaagcacaa gatgaaattt ctccaagcag cccacttgaa acagcgataa
      841 gctccgtaca agtaggtgac aaagtagctt cggggaatac ttttcaggag aatcctggtt
      901 atacgaaaaa ttataacttt tcggatttac aatttagtcc tcaagaacta actggagaca
      961 cactaaaagg caatacaatt ggatttgaag tttacggaaa acataatatt gcagcatcaa
     1021 ctaaaaactg ggaaatccgt cttcaattag atgaacgatt agctaaatat gtagaaaaaa
     1081 ttcaggtcga tccaaagaaa ggaattggat cttctagacg gacctttgta agaattaatg
     1141 attcgctggg tagacctaca aatatttgga aggtcaatta tattcgtgca agtgatggat
     1201 tatttgctgg ggcagaaaca accgatacgc aaaccgctcc taatggggta attacgtttg
     1261 aaaagagcct agatgaaatt tttaaagaga taggcataga caatctcaag accgatcgtt
     1321 taatgtatcg aatctacttg gtaagtcatc aagacgatga taaaattgta ccgggtattg
     1381 atagtacggg ctacttttta acagattcag atgatttcta taatagctta gatgtatctg
     1441 aaaataatcc tgaccagttt aaacatggat cagtaagtgc aaaatatgag aaacctaata
     1501 ctcaaacaaa agatggttca ggatccacag gtgctaatgg agctataatc ttagatcata
     1561 agttaacgaa aaattataat ttctcatatt catcttccgc aaagggcacg ccttggtatg
     1621 ctaattacaa aattgatgaa cgattagttc catatgtagc gggtatacag atgcacatgg
     1681 ttcaggctga caaagtaaca tatgatgttt cgtttgaatc cggaacaaaa gtagctgatt
     1741 tagcaatcga gcgacgggaa ggtcatgaaa attatggcat aggttcaata actaataatg
     1801 atttaactaa acttatcgat tttgctaatg caagtccacg tccagtcgtt attcgatatg
     1861 ttttacaatt gactaagcca ttagatgaaa tcttagaaga tatgaaagca acagcacaag
     1921 ttgaagaaaa taaaccattt ggtgaagatt tcatctttga ttcttggttg tcggatacga
     1981 ataaaaaatt aatccagaac acttatggaa caggttatta ttatttgcaa gatattgatg
     2041 gtgatggaaa ccctgacgat aaagaagaga gcggagacac gaatccatat atcgggaaac
     2101 ctgaattaga agaagtatat gatgttgaca caacagttaa ggggaaagta ttcatccacg
     2161 agttagcggg aacaggtcac aaagcccaac ttgttgataa agaaggtact gtattagcag
     2221 aaaaaactat cgctccaaat gaaaaagatg gggctccaat ttcagatact gtagaatttg
     2281 aatttacggg tgtagattca agtaaattaa tcgcgaaaga tgaattaaaa atccaaatcg
     2341 tttctccagg ttttgataaa ccagaagaag gttcaaccgt tattaaggaa tcaccaaaag
     2401 cggttgataa acaaaccgtg gtagttggat ttaaaccaga tgctaaagaa tcaattcgga
     2461 ataataaaaa cttacctgaa gatgcagagt attcatggaa aacagagcct gatacttcta
     2521 acgttactga tagtacgaaa ggtattgtaa ctgttaagat cggaaatcga actttcgacg
     2581 tggatgtaga gtttgctgta aaagcttctc aagctatgga aaatgatgca acatacgtac
     2641 ctataacaac aactccagaa acgacagttc aaagtggaaa acctacattt gataaaccag
     2701 atgttccttt agctaaagat gccttttcaa tttcagatgt ctataataag tacttcggta
     2761 atgcaagtgt tgatgcaaac actggtattg ttacattcac tccagctaaa ggtgtaggag
     2821 aatcggagcc gattactgga acaattccta ttaaaattgt ttaccaagat ggttctgtag
     2881 gcacgaccga tttagcagta actgtaagta aagatattta tgaaaatcca ggagaaaaca
     2941 ttcctgcagg ctaccacaaa gtaaccttca ccgcagg
//
LOCUS       DAYYBG010000168         2929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000168 DAYYBG010000000
VERSION     DAYYBG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.176"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACHAUN_002817"
     CDS             85..981
                     /locus_tag="ACHAUN_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8461444.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACHAUN_002818"
     CDS             1073..1288
                     /locus_tag="ACHAUN_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8461445.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACHAUN_002819"
     CDS             1306..1578
                     /locus_tag="ACHAUN_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8461446.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACHAUN_002820"
     CDS             1580..2443
                     /locus_tag="ACHAUN_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8461447.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          510 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAYYBG010000169         2848 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000169 DAYYBG010000000
VERSION     DAYYBG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.177"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..1559
                     /locus_tag="ACHAUN_002821"
     CDS             234..1559
                     /locus_tag="ACHAUN_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8461448.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="ACHAUN_002822"
     CDS             1552..1902
                     /locus_tag="ACHAUN_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461449.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="ACHAUN_002823"
     CDS             1899..2069
                     /locus_tag="ACHAUN_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461450.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
BASE COUNT         1011 a          448 c          542 g          847 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaa
//
LOCUS       DAYYBG010000170         2786 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000170 DAYYBG010000000
VERSION     DAYYBG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.178"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(177..1196)
                     /locus_tag="ACHAUN_002824"
     CDS             complement(177..1196)
                     /locus_tag="ACHAUN_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8461451.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1193..1417)
                     /locus_tag="ACHAUN_002825"
     CDS             complement(1193..1417)
                     /locus_tag="ACHAUN_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8461452.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1414..1707)
                     /locus_tag="ACHAUN_002826"
     CDS             complement(1414..1707)
                     /locus_tag="ACHAUN_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461453.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1744..1881)
                     /locus_tag="ACHAUN_002827"
     CDS             complement(1744..1881)
                     /locus_tag="ACHAUN_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8461454.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1883..2314)
                     /locus_tag="ACHAUN_002828"
     CDS             complement(1883..2314)
                     /locus_tag="ACHAUN_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461455.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2328..>2786)
                     /locus_tag="ACHAUN_002829"
     CDS             complement(2328..>2786)
                     /locus_tag="ACHAUN_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461456.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
BASE COUNT          776 a          576 c          450 g          984 t
ORIGIN      
        1 cataaagcca taaacaatca accagtagaa tgtgtggcaa caaacctgtt atcgcatagc
       61 ttggagtgtg actattatgg gtgatagtga agatatgcga taacatcact attttatcga
      121 atgattttta tagttgtcaa tatagttatg taatgctcct caacgaggag ctatttttat
      181 cgtttaggaa tatttaaata ccaacgtttg tcatggaaat cttgcgcacc gcctttagtg
      241 tttccctctg gatcattcgt tgcccgcatc atgacataga ctttcttatt agggaagtta
      301 cgcatgttaa aagatacatg atagccaaca tttccagaag tattataagc ttgatttaca
      361 tctgatctat aaattccatc agctcttact cgagctaatt ctttcccagt attgtaatcc
      421 ataatgaaga tatactcgta tttatagtta gcaatgtgcc atccagctac atgcaaattt
      481 gcattttcga tttctccaaa ctgatcaatg tgggcgtaat tcgttccatc tgtcagtgta
      541 ggattagctg caccagctcg tgttggatca atgacaggct tgttttcaga agttgttgga
      601 ttttcatcgg taaatccatg agctaaatca tatgctaatt tttctttact tacgcccatt
      661 tcagaaagat aaccgtaagg atctgtatga tcgccccaga tgttttgtgt tacccataaa
      721 tgcgatttga ttcccggttg gttataagga gtgtccaacg ttaatggaat accatatttc
      781 attgctgaat ctctagccaa ttcaacgtat gccttgtagt ttttctcaaa cgttgcttta
      841 tcatgtgtgt gttgtaactc aatctgcaca ggactgttgg cattagcata cgaaccagca
      901 ccgtactgta cataaccagg ttgaccgact tgataaacaa ttccgccgtc tcccacaata
      961 taagcagtat aagcgctagt ccatgaacgt tgcatatact gcgcttcatt gcgtcctgtt
     1021 gctgtttcat tagccgtttc atgcagtaaa atatactgat tatttgctac ttgtgagcta
     1081 ccttcatttg cgcccaaatt aaattcattg ttgatagtat aggcaaatcc gttaattggc
     1141 aataaaaaaa gagccgttaa caggctcatc gcagtaatag taattttctt tttcatttgt
     1201 ttcctccttc ttcgctttca gccgagaaca ttttgtaggt tcgatttgat acacccaaca
     1261 cactccctaa aaacgcgcca aaaccagtaa tgatgacaac acagatatct gtgtactgcc
     1321 aattgagcgc tttaccaact aaccccacga aagtagctag tgcgggaata attaccagtg
     1381 cgaaccattt tagtacttcg aacgttttat tattcatttt cttctctccc taaacaaagt
     1441 tttgatttgt tgcgtgtgtt ccaccaattt ttctgtatgt gtatctaatc tttcatcgtg
     1501 tttctttagt tcttcatgaa tcatcaatcg atctgatttg ctcgattcta aatctttagt
     1561 cagcaaatct aaattgtggc ttacttttga aagagtctca gtaattttcg agaaagatgc
     1621 agcaatcggt ctaattacta ataaaatcaa agaaacgata gcggttattg atcctgctat
     1681 cattccccat tcccctaaat taatcatgtg acaactcctt taccttaaat aaaaacgcat
     1741 caattaagat gcgctcttat ctttattaat gattttatct gcttcttcgt ctgtaatgca
     1801 taatggaacg aaaaccatta cttgttcgtt agtgaaacag ccccagtcat acattagttt
     1861 gatatcttca tatgaataca tgttacttcc caccttgtaa ttgttttttg atttcttcga
     1921 tttccttggt attttgtaca gaagcaagca tcgtttttga attcaactgc gctaaacttt
     1981 ctgacttttc tttcaataag acattttctt gcttgattgc tacattgttt agcattgctt
     2041 tagaatttag ctgtttcaat tcatcatttt ctgccttcag cgtttcgtat aatgccttaa
     2101 tattagccaa ttcatctacg ctatcaacag cattcccttt tttctcttct tcagtagcta
     2161 actcaaccca ctgtttttta tcaaaatcaa atcttggtct ccagttagga actggtggtt
     2221 taatctctgt acagttttca gggatatttt cttgattgtt caaaatgatt tgctcgaatc
     2281 cgtaaggctt gattgatttg taaactactt tcattagatt tcctccctta aattgaataa
     2341 gtgataacaa atgaataagc tgatccataa cttgaatttc ttctccattt aatggctccg
     2401 tctgcgccaa tagatagttg agcactgttc aaagtagaac ggtctattga cccaaccagt
     2461 tgttcaaaac taattggtgt ccgatagcct tctggaacag ttaaaataac cgaatcattc
     2521 ccgccactac tttttccgtt taaagccaca aaatatatag aaactgtttt tccttcacga
     2581 taaagctttg ctgtccctgt attaccgtta gtgacaacca gatttaccac ttcatatctg
     2641 ttatcatcag tcgtcaaaac acgtttatta ttgattgtta cagaacctga aaaattctta
     2701 tgtcctgcaa tggcttggtc tccatctgtc tgcaccaatg cttctttacc atcaatagct
     2761 tctacatgcg tttttaagta tttagc
//
LOCUS       DAYYBG010000171         2757 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000171 DAYYBG010000000
VERSION     DAYYBG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.179"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1300
                     /locus_tag="ACHAUN_002830"
     CDS             <1..1300
                     /locus_tag="ACHAUN_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8461457.1"
                     /translation="GGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKY
                     EPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTY
                     PDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYA
                     WKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNK
                     EPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1434..>2757
                     /locus_tag="ACHAUN_002831"
     CDS             1434..>2757
                     /locus_tag="ACHAUN_002831"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8461458.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVT"
BASE COUNT         1081 a          372 c          537 g          767 t
ORIGIN      
        1 cggcggtaca attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta
       61 tccagacggt acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga
      121 cgccgataaa tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga
      181 tttaacagat aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt
      241 tactcccggc ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt
      301 aacgtatcca gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg
      361 ttctaatgca gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga
      421 gccagatgca tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc
      481 ttggaaagag aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac
      541 atatccagat ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc
      601 cgatgcagat aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc
      661 agatgcatct gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg
      721 gaaagaaaaa gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata
      781 tccagatgga tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc
      841 gaataaacct caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga
      901 accaaatgca gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga
      961 taaaaatggt aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt
     1021 aactgcaact gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa
     1081 caaaaaagac agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa
     1141 cagcaatcaa gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg
     1201 agagagtgac agtaatatct ttactattag cggtggttta atactgttag gaacgttagg
     1261 attattaggg tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct
     1321 tctctgagaa gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa
     1381 gtgttagtta aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa
     1441 aaatagtaat tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa
     1501 gcgattcaaa ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat
     1561 gtgggacatc gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa
     1621 atagaatgac actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa
     1681 ttgatattca aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag
     1741 aatatgacaa agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga
     1801 ataatttagc taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag
     1861 ctttggatga tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg
     1921 aaattgctga agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata
     1981 gagtgttgtc gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa
     2041 aaaatggaat acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga
     2101 ttgaaaaaat agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta
     2161 taggatttct atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag
     2221 catatctaac taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt
     2281 gtgctacaac tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg
     2341 ctctaagaac agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg
     2401 gttcccaatt tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga
     2461 atttaaaaac agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca
     2521 ttagacctgc ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa
     2581 atacaaagag aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat
     2641 caatgttttc tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt
     2701 atttatttca tcctagtttt agccaaccct ataattttat tttaaaggca gtaacag
//
LOCUS       DAYYBG010000172         2744 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000172 DAYYBG010000000
VERSION     DAYYBG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.180"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..931
                     /locus_tag="ACHAUN_002832"
     CDS             <1..931
                     /locus_tag="ACHAUN_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8461459.1"
                     /translation="FWISLPLCLIERFMVTYPTGTIVGDMIGGVFWNYSGCNMPLNLI
                     LCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFIIFVAIVFTLKV
                     DFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLEYASRDSGYDGV
                     GDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSFGNLHYQYFHHI
                     WLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVFFFVYNTTLRGE
                     TTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            928..1647
                     /locus_tag="ACHAUN_002833"
     CDS             928..1647
                     /locus_tag="ACHAUN_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFM8461460.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1662..>2744
                     /locus_tag="ACHAUN_002834"
     CDS             1662..>2744
                     /locus_tag="ACHAUN_002834"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8461461.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
BASE COUNT          960 a          299 c          541 g          944 t
ORIGIN      
        1 attctggatt tcactgccac tttgtttaat tgaaagattc atggtcacct atccaactgg
       61 aacaattgta ggagatatga taggtggagt tttttggaat tattcaggtt gtaatatgcc
      121 attgaattta attttgtgtg tgacagttac tgttgttaca atcggatatt tcaagggaga
      181 aaaaaatttc atccatttta ttttatgtgt ttgtgcagca ctttttatgt ctgcattagc
      241 tgaattgaaa gtttttattc ctgaattatt tataatattt gttgcaatag tattcacttt
      301 aaaagttgat tttaaagtag tgatcaagat attatcatgc gtaatacttg tgtcgatagt
      361 attatccttt tttataacaa tatttattac attgaacgat aatggacaaa gctatgctga
      421 taatttcaca attcaaggtc ttttagaata tgcgtcaaga gattctggat atgatggtgt
      481 tggtgattta aatcgaataa ctggtatctc tagagtagta gatgaaatat ttaataataa
      541 ttttagatta gtcctaatgg gagtaggcat tggcaatgca gagtttacca atttttttgt
      601 cagtgagttt tataaatcgt ttggtaatct tcattatcag tattttcatc atatttggtt
      661 atttatagaa gttggaatat taggagtttc gatattccta ttttttataa taaagacatt
      721 tttcaatgca aaaagatctt ttatgaagga taaagatgca agtcttgtta aagttattgc
      781 aattatgtcg gtattctttt ttgtgtataa cacaacactt aggggtgaaa caacggggcc
      841 actgatttat atgattttag ccatgcctga agttgtgaaa aggaaagagc attattatag
      901 tgaaaaaaat acaaaaggag agatattatg attcctaaga agatacatta ctgttggttc
      961 gggggaaatc cacttccgaa aaaagataga aagtgcatag aaagctggaa aaagtattgc
     1021 cctgattatg agattattga atggaatgaa tcaaattatg atttaaataa gaaccgttat
     1081 atggccgatg catataaaga acgtaaatgg ggttttgttc cagattatgc aagatttgat
     1141 attatttata atcatggtgg attttattta gatacagatg ttgagctaat taagtcgtta
     1201 gatatacttc gtgaaaatca tgcctatatg ggatttgaag gggaaatctg ggttaatggc
     1261 ggtattggtt ttggtgcaga agaaggacat tctgttatta aaggtttaag ggatatttac
     1321 gattcattaa gtttttatca aaatgatggt acattgaact tgaaaccgag tccgtattat
     1381 attacagatt atcttgaatc tattggattg gttcgcgata acagcgaaca aataataaat
     1441 ggcctgcata tctatcctac ggattatttt gctgccaagg attatgatac tggaaaaatt
     1501 tataaaacta gtaatacaat aagcattcat caatataatg catcttggaa aacacctaaa
     1561 caaaaatttg tttatagatt aaaatcaaca attggacaaa aaaaatttaa caggttactt
     1621 gcactgaaaa aatttttaca cttataaagg agaaaagtac tttgaagaaa atttgtttca
     1681 ttagtggtgt aatttctcgt tctgggggaa ctgaaagagt agggagtatt attgccaata
     1741 gcttgtctga tttaggttat gatgtcacta ttttaagctt ttggaatagt ggagagcctt
     1801 attttgggtt agataagacg attaaggttg attatatgtt aaatcctaaa acggagggaa
     1861 aattatatag aacttattta tatccggttt ggaaattgca caagtatata tgcaaaaatg
     1921 aatttgatat agtaattgat attgatactt tgttgtcttt gtatactaca agggcaatta
     1981 aaggaacaaa gtgcaaattg atttcttggg aacattttaa ttactggaca atggaaatgc
     2041 tgaaagaaaa gaagagattc aaggctaaaa aattgataaa aaaatatgca aagaggcttg
     2101 tggttctcac agaagaagat cgtcaaaaac atattgatga atatagttta tcagaagatt
     2161 ttgttataac tatgccaaat ccgtgtatat ctaatatcga gtacgaatat cattatgata
     2221 aaaagagatt tcttgcagta gggcgattag tggaagagaa aaattttgct gacatgataa
     2281 gtgcttggaa aattgtagaa aaaaaagtag atgattggga tcttatcatt gtaggaaaag
     2341 gtgaattaga aaaagatctt cgccagcaga taaaagattt aaatctgcaa agagtaacta
     2401 tagcagggca ttcagaccat gtggaggaat attataaatc tgcatcttgt tatgtgctta
     2461 gttctgttta tgaaggattt cctatggtta tattagaagc acagtcttat ggattaccag
     2521 ttatttctta tgattgtaaa actggtccga gagatttggt acatcataat tttaatggga
     2581 tgttggttga agataagaat atagaccaat tagctaagag catgataatg tttactaaga
     2641 atactgattt agcaatgaga atgtcactga acgcatataa taatgtacaa aaatttaatt
     2701 tgaaagaaat tacaaaacaa tgggttgctt tgattgaaaa tgta
//
LOCUS       DAYYBG010000173         2677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000173 DAYYBG010000000
VERSION     DAYYBG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.181"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..367
                     /locus_tag="ACHAUN_002835"
     CDS             44..367
                     /locus_tag="ACHAUN_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461462.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            531..785
                     /locus_tag="ACHAUN_002836"
     CDS             531..785
                     /locus_tag="ACHAUN_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461463.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            855..1100
                     /locus_tag="ACHAUN_002837"
     CDS             855..1100
                     /locus_tag="ACHAUN_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8461464.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1176..1541
                     /locus_tag="ACHAUN_002838"
     CDS             1176..1541
                     /locus_tag="ACHAUN_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8461465.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1602..2165)
                     /locus_tag="ACHAUN_002839"
     CDS             complement(1602..2165)
                     /locus_tag="ACHAUN_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461466.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          785 a          474 c          422 g          996 t
ORIGIN      
        1 tttgcttatt ttagaaaaaa tgttattctt ttatttattc tcaatgttag gagtgattat
       61 tttgccattt attgcgattt tattcgattt agttgcggca gcagcttatt ttctccaatt
      121 aaattatcaa acctcaggaa ttctgtttat tggaatgatt tttcaaggtg tgattaccct
      181 tttgcttctt gtcatgatgc tcagctataa aggaaaaaga tatgcccgta ttcaaacaaa
      241 agtatttgtt aaatatgttt ccattcgcta tgggatcatc attctttcct ttttagtgaa
      301 tgcgatcgtc ctctttttat atgtattgaa ttacttagat atcaatccat tgattttttc
      361 tcgatagata agacacattc agcggaacag ttcctgctta tcagcagaaa actgttctgc
      421 tttttcattt ttcattgatc tattcttctt ttattttcct agcatttcat ttgtgatgta
      481 tgcaataaca tgttatcttt tattatatga atcgttgagg agtccgttac atgattacgt
      541 tggtgagagt tttgttttgg gtgcctgcgg tcgcactggt cgcaagtatt gtttatctta
      601 tgaattggaa caaagaacgg ttttatttag ctattttgac tcttccggct atctacttta
      661 tgtggaaagt ttttaattac aactactttg aacccgatag tgtttttata gaagaattat
      721 caggactggt tttgtcttta ttgatcgtca ttctttattt gatacgttta aataaaaaac
      781 attgagtact attgattgca tgtgtagctt tctatagtca ttataactat tctactataa
      841 tgaaaggtga ttaaatgagt atcgaagaag ctattttact ggatacttta cgttgtcgtc
      901 tgaatttctt aagaactggc cgatctttat ctcaagtagc tgtttctagc gcgttaagta
      961 ttcctttatt caaatatcgt aagtacgaag aaggagaaga aattccaagc atcattgaat
     1021 taatctatat cgctgacttt tacgatattt ccgttgatta cctaattggt cgctcagaaa
     1081 cagcaagcaa actgaagtga ttttttccag ccttggtgct ggttttttca tttaacgtct
     1141 aaggttaaca atctatatat aaaggagaca tttctatgcg attatggcat gaaaaattga
     1201 tttcaaagct tcctcgtcaa cagcttttag gacaacatcg ggaatgctgt gcattaagag
     1261 gaaatggctg gggaaagaaa catgctactg tggattatgt ttttcactat tctccttata
     1321 gattattcca atatcatcag cttgtcatgg atgaaatgga aaaacgtggt tacagcccag
     1381 caccagagtg gaaaaatcct atgtatcgtg gcaaatcatg cgaagcattt tttgatttac
     1441 ctactgtaga gcgcaacgtg ccgatttatc cggaacacga tgaagtttat ctagcagaat
     1501 gtttagcaaa tttggagcaa aaaggaattt atcttaccta atataactag aaaagccttc
     1561 tatctcctct tacttcatta aaaagcttta accatcttag atcaagctaa aatgtcgtaa
     1621 gttttagggt tatcttcatc ctcacgtgca aaactaaaag cgtactctag ttttggttca
     1681 tcgccaactt ccctagacat aaatgaacat ctcctgacgt ggtttaaaag taccgcatct
     1741 tcgacgtctg tatacgatgc acagcacaga ttgcttcttt tgatctttct tttgattttt
     1801 ctgttgcttc ttctacacct tcttcgatta ctacatcgat gcttacaatt gcttctgcct
     1861 aatattccca tgcatcgtcg atttaagcct ctgtagtcac tgtgatcgtt tccacatttt
     1921 aactcctctg tcgtggcttc agttgatttt gtgatcagtt cagttgctaa gccagagtca
     1981 gaacaacctg ccattgctag cgttaatatt gatagtaaaa atagtttttt tatcataagg
     2041 ttccccccct gaaaatagac taaaataata acgctttctt tttttgtttt cagacgaaaa
     2101 ttaataattt ctaaatattc tttaaaaaag cttataacta ttatgccttt tttaatatat
     2161 aacacagtta tttttaaaaa taagaatgct aaatcactct acaataacgg cgaaagcaca
     2221 aaaaataaaa aaattattac gttattaaaa aaacagatga aagatcaggt tttattagat
     2281 ctcattcttt catcacttga agggatttaa tgaactactt tttcctcttt gtcgttttta
     2341 tatattcgta ttccttcaaa cagtaggata gcctctttca atgcttgata tttgaactgt
     2401 ccgttactta aaaatttaga tgtgccgccc tcttcctaga acatattctt ttttactttt
     2461 tcattaattg acagaccaat cgaacgaaac tgtaaatttc gataggataa tgattggtca
     2521 aactcattca ttttcaagac atctcgattc atattcaaga taggaatctt tttatcaaca
     2581 tcactttatt cgtattttcc tctaccataa aagcttcacg ctccacaaaa ttgttttata
     2641 cactactatt cagcccattc aacgaccaat gaatttt
//
LOCUS       DAYYBG010000174         2633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000174 DAYYBG010000000
VERSION     DAYYBG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.182"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACHAUN_002840"
     CDS             complement(137..427)
                     /locus_tag="ACHAUN_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461467.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACHAUN_002841"
     CDS             complement(545..805)
                     /locus_tag="ACHAUN_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461468.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACHAUN_002842"
     CDS             complement(942..1355)
                     /locus_tag="ACHAUN_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8461469.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="ACHAUN_002843"
     CDS             complement(1811..2296)
                     /locus_tag="ACHAUN_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461470.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2632)
                     /locus_tag="ACHAUN_002844"
     CDS             complement(2435..2632)
                     /locus_tag="ACHAUN_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8461471.1"
                     /translation="MALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKIIYIVVG
                     ICAIYCLKFFPMITRKVDERY"
BASE COUNT          975 a          437 c          406 g          815 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca ata
//
LOCUS       DAYYBG010000175         2619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000175 DAYYBG010000000
VERSION     DAYYBG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.183"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..317)
                     /locus_tag="ACHAUN_002845"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="ACHAUN_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(349..672)
                     /locus_tag="ACHAUN_002846"
     CDS             complement(349..672)
                     /locus_tag="ACHAUN_002846"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM8461472.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            1134..2516
                     /locus_tag="ACHAUN_002847"
                     /pseudo
     CDS             1134..2516
                     /locus_tag="ACHAUN_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          940 a          456 c          433 g          790 t
ORIGIN      
        1 tttcgtaacc agatatctaa ggttaagccg tccgcatcaa ttgcacgata aagataatgc
       61 caacgtccct taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta
      121 tttttcttct tccaaagata atagaggact ttgctgtact cttgtaccca acgataaatc
      181 gtagtatgac aaacatttat tccacgatca tatcacaatt cctgaacttc acgatagctt
      241 agattgtaac gcaggtagta accaacagcg acaataatga cgtctttttt gaattgtttg
      301 cctttaaaat gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat
      361 agattttttg gaaaactttg caacagaacc gaaatgagta tagtatgaat tagtaatttg
      421 gttttcatct aagtttagct ttgatttact aaaattgata tctcgataca tgttcaatac
      481 atctaataca aatcgagaat cttctttcgg aaatggctca gaaagatcac tccaaatttc
      541 atcatcataa tgatactcgt aaccttctct caatatttcg atttttcttt cataataaga
      601 tctgttttgg tcctcaaatt tagccaataa ctcaaattga tttactagtg acaatctctg
      661 aaaactgtcc attaaatgtt tcttcctatc ttgttcatta acaaaaaatt ataagccctt
      721 acaacgaata ttatatatta aatttaattg taggtgaaca cgaattttat aaatatttat
      781 ggtgttaatg acaaatgata tccgactcct cttcatacgt tctggactat ttgctaatag
      841 attaaaattt cagcatctat ttctgaccat aaataaatga gctgccgaaa agctgccaaa
      901 tcagacttga aaataaagga tcaaaaactt ttaagtttct gaccccgtaa ctcacacttc
      961 aaaatcttac ttaaaaagga gtctgaaaga aaaaaagagt attcaaaatt tttttctaac
     1021 tagctacatc acatctatgc attgacactc aaagaaccag aataagtcat cagaaagcaa
     1081 gggggtgcgg acataatcct ggacgaaaaa ataaacttag gaggaatgtc cgtgtgtttt
     1141 cccacaaaga gcgtgtaagg gcaattcagt tatttttaaa atacgattgc tcttatgctg
     1201 caaccattcg agaattaggg tatccgtcga ttggtgcact acggaaatgg tataaagagt
     1261 atttggaatc cggagagctc catcaggagc accgaaaaaa atcaaagtat tctgaagaac
     1321 aaaaacgaat agcggtgaat cattattttg aatatggtca atgttatgca cgaaccattc
     1381 gcatgcttgg ttatcctaat agagagtcct tgcggcagcg gtgtgaagaa cttgcaccag
     1441 gagctagaaa actccgaaaa agtgcggtaa agttaacaca agatcaaaaa gaagctgtgt
     1501 tgaaaaaatt ctataaaccg caaactaata ggaaaaattt agccgaatct ggaggaatta
     1561 gtagagttac tctatatcaa tggaaaaaca cggttcttgg aaaggatttt ccattgtgta
     1621 tgactttaaa aaatcaggaa aaacaaaaag agctactttt aaaggaagta gaagacttac
     1681 aaaaacaagt acaccaatta cagctcgaaa aagcattact cgaaggagcg gctgaactgc
     1741 taaaaaaaga aaaaggcgtc aatcttcttt gtctatcaaa tcaagaaaaa acgatattga
     1801 ttgatgcctt acgaaatcag ttcaccttga aagagcttct tcaacagctc cagtttccta
     1861 aaagcagcta tttttatcaa aaacaagcac ttgagaaacc agataagtat tacaaagaac
     1921 gtcaaattaa ttatcacgat tttcaatcat aacttctgtg cgtatggtta ccgtagaatt
     1981 caccaagctt taaaaaatat gggtaaaaag ctttctgaaa aagttgtacg tagattgatg
     2041 actgaggaaa atctatttgt taaattttct agacgaaaaa agtattcttc gtatgctggg
     2101 gaaatttctc ctgcacaccc caatttatta aatcggaatt ttaaagcaaa gaagccaaat
     2161 gaaaaatggt taacagatat tactgaattt agaattctcg cagggaaaat ctacttatca
     2221 ccacttgtcg attgttacga tggtgctatt gttagttgga ctattggaac aaagccaact
     2281 tgcttcactc tatgtctaaa aagagttatt caccagataa ttcagcgtgc gaaggtttct
     2341 ttggacgatt aaaaaacgag atgttttatc aacgtgactg gaagaataca acaatcaatc
     2401 agtttataaa caaattggat gattatattc attggtataa taatcaacga attaagttat
     2461 ctttaggaag gctaagccct cttcaatatc ggaagaagca aagatatatt tgttaaaaag
     2521 gtccaaaaaa aatatccgca cccccttttt ttgtaaaaaa ctattgcaca atacaagtag
     2581 cctacggcta tcgaaatttc cataactttc gagcaagaa
//
LOCUS       DAYYBG010000176         2592 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000176 DAYYBG010000000
VERSION     DAYYBG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.184"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..2524
                     /locus_tag="ACHAUN_002848"
     CDS             83..2524
                     /locus_tag="ACHAUN_002848"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8461473.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          865 a          461 c          611 g          655 t
ORIGIN      
        1 taatcttaga aaaaaataac tggaacaaga gctatttgtt tttacagcaa agaatggtat
       61 atttttgtag gaggtggctt acttgaaaga agtcgttatg atagatgctg caagaacgcc
      121 gataggaaag tatcgcggaa gtttaagtcc atttactgca gttgaattag gcacacttgt
      181 gactaaagga ttattagata aaacaaaact gaaaaaagat aaaatcgatc aagtaatatt
      241 tggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc agacagattg ctttgaacag
      301 cggtctccct gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat ctggtatgaa
      361 agcagtgatc ttagcacgcc agctgatcca attaggtgaa gcagaacttg tgatagccgg
      421 cggaacagag agtatgtctc aagcacctat gctgaaacag tatcagtcag aaacaaatga
      481 atatggtgaa ccaatttcca gtatggtcaa cgacggattg actgacgcat tttcaaatgc
      541 acatatggga ttaaccgcag agaatgttgc aacacaattt tctgtgagca gagaagagca
      601 ggatcgctat gccttgtcgt cccagttgaa agcagcacat gctgtcgaag ccggtgtatt
      661 ttctgaggag atcatcccag tcaagatttc tgatgaagac gtgttatctg aggatgaagc
      721 agttcgtgga aatagtacat tggaaaaact gggcacgtta cgtacagtat tctcagaaga
      781 aggaactgta acagcaggaa atgcttcccc gttgaatgac ggtgcctctg tggtgatcct
      841 tgcatccaaa gaatacgcag aaaataataa tctgccttat ttagcaacca tcaaagaagt
      901 agcggaggtc ggtattgacc caagtatcat gggaatcgct ccaatcaaag ccattcaaaa
      961 attgacagat agatcaggaa tgaatttatc aacaatcgat ctttttgaga tcaatgaagc
     1021 atttgcagct tcttctatcg tagttagcca agaactgcag ttagatgaag aaaaggtcaa
     1081 tatatatgga ggagcaatcg cattaggcca tccaattggt gcaagcggtg cacgtatctt
     1141 gactacatta gcttacggat tgcttcgtga acagaagaga tacggtattg cttcattatg
     1201 tattggtgga ggtttaggct tggcagtctt gttagaagcg aatatggagc aaacacataa
     1261 ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct gaacggcgca gtcaattaat
     1321 agaaaaaaat gtattgacac aagaaactgc gttgattttt caggaacaaa cactttctga
     1381 agagctctcc gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc caatggggat
     1441 cgcgcagaat tttcaaatca atggcaagaa aaaatggatt ccgatggcaa cagaggaacc
     1501 atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct gcgcagaaac
     1561 acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt atcaagcagt
     1621 gatcaatgca gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg aaagttatcc
     1681 ttcaatcgtg aaacgcgggg gcggtgttca agatatctct actcgggaat tcatggggag
     1741 tttccatgca tacttatcta ttgatttttt agtggatgta aaagatgcaa tgggcgctaa
     1801 catgatcaac tcgattcttg agagtgtggc aaacaagtta cgggagtggt tccctgaaga
     1861 agagattcta ttcagtattt taagtaattt tgctacagaa tcattggctt ctgcctgttg
     1921 tgaaatccca ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa ttgccaaaaa
     1981 aatccaacag gcaggggaat atgcgaagct tgacccttac cgtgcagcaa cgcacaacaa
     2041 agggatcatg aatggtatag aagctgtagt ggcagctaca ggaaatgata cacgtgcggt
     2101 tagcgcttcg atccacgcct acgcggcaag aaatggatta taccaaggat taacagactg
     2161 gcagatcaaa ggggataaac tagttggaaa actaacggtg cctttggctg ttgccacagt
     2221 aggcggagct tccaatatcc tgccaaaagc aaaagcatct ttagctatgt tagatattga
     2281 ttcagccaag gaactagcac aagtaattgc tgcagtaggg ttagctcaaa acctggctgc
     2341 tttgcgtgca ttagttacag aaggaatcca aaaaggccac atgggacttc aagctcgttc
     2401 attagcgatc tcaattgggg ctattggtga ggaaatcgag caagttgcga aaaaattacg
     2461 tgaagcagaa aagatgaacc agcagacagc tatacaaata ttggaaaaaa tccgggaaaa
     2521 ataaaaaagc aatatcttta gaagaataaa gcaagattta cagttatttt atttactgca
     2581 gatcttgctt tt
//
LOCUS       DAYYBG010000177         2580 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000177 DAYYBG010000000
VERSION     DAYYBG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.185"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..65)
                     /locus_tag="ACHAUN_002849"
     CDS             complement(<1..65)
                     /locus_tag="ACHAUN_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HFM8461474.1"
                     /translation="MTFNIIELENWDRKEYFEHYF"
     gene            complement(195..1133)
                     /locus_tag="ACHAUN_002850"
     CDS             complement(195..1133)
                     /locus_tag="ACHAUN_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8461475.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRLWK"
     gene            1624..2580
                     /locus_tag="ACHAUN_002851"
                     /pseudo
     CDS             1624..2580
                     /locus_tag="ACHAUN_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT          890 a          445 c          402 g          843 t
ORIGIN      
        1 ttaaaatagt gttcaaaata ttcttttcta tcccaatttt ctaattcaat aatattaaaa
       61 gtcatatata acttcctcct aaattttaaa tttttatatt taggaggaat aatcctctga
      121 ttttttcata cgttatgtca cctcgtaaat attaattata ctgaattagc aatttttatc
      181 aaataaaact tattttactt ccaaagccta aattcacgtt gccaaaaatc aatctgcttt
      241 tgcaattgtt tttcgttcgc ttttaaagtc gatttcatta attccgttaa atcaattgga
      301 gatatttctc taatcaattt tttaaattta gtcttagtat tcttacttag ctttccccac
      361 atactttctt catgcaacaa agtataaacc atagcttgct cattaatttt ttctaaagta
      421 gcccacgcag gtttcaagat gtgtaaatca ttaaaacaat cattccagta atcaaccata
      481 tctcttttta attcaacttc tacacgccat aaatgttcag acacaacttc aacatctgcg
      541 ttatctttac gttcttgttt tttattataa attctaataa atctatcact atcacggaca
      601 ccaaaatatt ttgtttctgg cttgccatta cgaccataaa aaacagtttt cttaactgct
      661 ttatcagtca ttgcatagta atcgctcaaa tcatcttcaa aatcaaaagc taagtctaat
      721 cttgtaaaac cgtcatcttc catgtagtcg ataatatttt gttttaacca aatcatttct
      781 tcatgtgtga gtttattggg attaaattca acacgcatat tacgtctatc ccaagtatct
      841 gcttttactt tgtcatattc gatataaact ttttcttgaa gtgccttagc tttaaacttt
      901 gtttgaagta tatcccaaag tcgtatttgt ggctctacac tcataaagtc agatagcttt
      961 ttagcattag ttttgttcaa atttccaacg attgtcatgg catcaaaact taatgcgggt
     1021 tgagattttc ccaaagtttg accacttaac cggctattac ttaaccggct attagagacg
     1081 gccggttttt ttagtgagat ttctgcagct aatttttcat caattttctt cattttttat
     1141 cgctcctaaa ataatttttg gaacgaaaaa aggacgagtg gaatcgcaaa ataaatttcg
     1201 cctgtatcca ttcgtccatt ttgttttgta ttcaaaatca aaaattgata tactaatcat
     1261 cgaatgaata acaagtgtgg tttgttattc cgggcgagaa cgttggtagc gttgctcgtc
     1321 tttttttatg tctttttttc gttaaaacta ttttaaaact aaatatatct taaagcaaat
     1381 aaattcaagg ggctaaaagc gccccttaaa aaccccttgc ctctgaaaag ttttgtgttt
     1441 cctttccgtc ggtttcaaaa gctaatttac tcaaaaaaat agatcaaggg taaatgcact
     1501 cactgcgttc gcccttgagc tattttattt cgcaaatttt aacgcatttg ccgacgaaaa
     1561 gataaaacac ataaaaatat acgcagatat atttaaaacg tcttagaacg caaaatttta
     1621 cgtttaaaag ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat
     1681 acaccattta ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc
     1741 ggttttttag gacttgatct tgaaatggtt caaacagagc ctagaaagcg ttatgtggac
     1801 ggtagtaagg tacaggaaca ccacgccatt atcccaacaa aacaagtacc taccgaatct
     1861 gcattagcga aaatggacga tttacaacga aaaatttatg ctttagtcgt taaaacgacc
     1921 gttgccatgt ttttacctga ttacttgtat gaagaaacca agatacaaac gaaagtagct
     1981 gatttgctgt ttcaatcgat cggaaagaca ccaaagcaag aagggtggaa aattcttttc
     2041 aaacaacaaa ccaaagaaga aaaagaggac gttcaaacgt taccactcgt tatcattggc
     2101 gaacgtgccg aagttggtgt taagagtgtt gaaaaagaaa cgcaaccgcc aaaagctttt
     2161 acagagggta cattattaac tgctatgaaa acggcgaata aaacggttga tgatgaagaa
     2221 gcaatcaaga ttttacaaga agttgagggg attggaacag aagcgacaag agcaagtatt
     2281 attgaagcgt taaaacaaaa agaatatatc caagtgatta agaataagct tgttgtaact
     2341 gaaaaaggaa aattattgtg ccaggcagtt gaaagtcagc accttttaac gagtgctgaa
     2401 atgacggcta aatgggaaac gtatttaaaa aaaatcggta aaagagaagg caatcaagag
     2461 aactttatta cgaatatcaa aaaattcatt gttcatttac tggaagctgt acctaacgat
     2521 atagaaaaac taaatttttc tgattaccag gaacagaaag aaaaagaagc agaaaaaagt
//
LOCUS       DAYYBG010000178         2489 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000178 DAYYBG010000000
VERSION     DAYYBG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.186"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..392
                     /locus_tag="ACHAUN_002852"
     CDS             156..392
                     /locus_tag="ACHAUN_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFM8461476.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            411..626
                     /locus_tag="ACHAUN_002853"
     CDS             411..626
                     /locus_tag="ACHAUN_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFM8461477.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1329..2081)
                     /locus_tag="ACHAUN_002854"
     CDS             complement(1329..2081)
                     /locus_tag="ACHAUN_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8461478.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2096..>2489)
                     /locus_tag="ACHAUN_002855"
     CDS             complement(2096..>2489)
                     /locus_tag="ACHAUN_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8461479.1"
                     /translation="KLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEA
                     SIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIE
                     EINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          806 a          396 c          414 g          873 t
ORIGIN      
        1 acagttcttg aacactaact ttttcattcc tgaatcaaat ttattttgga aattttacta
       61 ttcaagaata aacttgataa gtttcaggaa gatttttact gttaatcaaa tacgtatgtt
      121 acagtagcac cgtaaaaaat attatttaaa gaagcgtgtt taaacatgca aaatgtaaag
      181 gaactaagta ttgtggaaat gcacaaactg ttggtggagg caataatgct tggggaaaat
      241 tggggcaagt tgtaggtgga cttaccacag gggcagtagg tggtgcaggt ttaggaacag
      301 caatttgcgg accagcttgc ggtgttgttg gagggttata tggagctgtc gctggaggtg
      361 ctgcagctgg ctgggatgct cgaaaaaaat aaaaaaggag aataagtagt atgcatatta
      421 aaaacacaaa aacaacattt atattatctt cagaagagtt gaaaaatatt caaggtggga
      481 gtgccgtggg agttttaggc actacttttt ctggggcaac agcaggagta aaactttgtt
      541 cagccggagg accttatgca atagctgctt gtggtgtagg tggagcttta ttaggtgctg
      601 gattcagcat gtggaccggt gcgtaaacaa ttaaaaaata tcaaacctca agattggttt
      661 gtgtcgatta cgaacatagg tatgttcttt atcttttcaa aagtttggaa tggacagaac
      721 tttttactaa agcttcttgt gctagttttt ataattatat taaattttta tcttttatta
      781 aagtcagata aaaattcttt ggaaatgtaa tggtagagat atttttacaa aaggacttgc
      841 agcgctgtag ttggtgctgc aggaggacct atttcggccg ttacaggagg tattggtaat
      901 gtacttgtcg gatgtgcatt aaagcctgtt cctgcaagtt aaatttataa aaacaggaaa
      961 gtaattatct caaatcctat caagtacaat cataggattg cacttgacag gacaaatttt
     1021 ttaactaaaa aatgaaaaac actttctaaa aattagtaga atttgccaaa tctttcgcat
     1081 cttccaccat acatttaatt ttaattcccc tttatggtac ttaataaaaa caagaaaaga
     1141 actattcatt tggccctttt ttggttccag accatagaaa tattcctcca gtaactaata
     1201 ttgctgttat gcacgtaact aataacagct ataaaaattg gtacaacttt aataggattc
     1261 agaaaaattt gcactagtga aggaatctat tttattagaa aaatgcaatc tccctaaaga
     1321 aaaaaccact aaacagtgct ttctttcaac ttctccttaa ttttttgtgc tttccctaaa
     1381 gtaaagtttc tggccaactc tgaatcaaaa tatagtgaac gttttttaaa atctatttct
     1441 ttaacgttaa agatatttac taaacacgct cgactaattc ttgttaacat aggatatttc
     1501 ttttcaagct cactaattct tccataaaat tcgtactgcc catctttagt acacaatgat
     1561 aatctatgtg gtaattttga agcttctaca aaataaatgt catttatatc aaaagtaaat
     1621 gtttgagaac ctatggaaaa aacaaatgct tgattcgtaa cctgttttgt tgcatctatc
     1681 ctttcttgcg ctaataacaa taactcaaca atctcgtttc gatactcatc tagtgtttga
     1741 tcttttgtta caaatcctaa cgtttcaact cttcttttta ttgttacggg taacatctca
     1801 tcatgagtag ttgtaaaaat gatttttgct tgaacatcat attttctgat tacttctgct
     1861 aattctatac cgttaacttc atgatttaaa tcaatatcta agaaatatat tccttgcttt
     1921 ggtcggaatt gtttgaggta ttttttaact tctaacggac tttgtgtttt caatactatc
     1981 ttaaatactt tatcgtgaaa aagaatgaaa ttatcaataa ttcgttctaa ctgatttaat
     2041 tgtataattt gatcttcaca taatattatt ggataactca taaatcctcc ttccctcaaa
     2101 acaacaaaat aacttgagta gtaaattgat acggatcgtt ttgatagttc acaaacatat
     2161 tgggattctt tttattgatt tcctctatac tagataaacc taacccttga tgtccctttt
     2221 ttgttgttat tccttttttc attagagtat ctatagagag tagtatctct ctacaactat
     2281 tcgatatcga aatttctatc tgtttatcat ctcggtaaat agctagagac agttctctat
     2341 cttgactctc tatagatgct tcaattgcat tatcgagaat aattccaagc accctaatac
     2401 agtcaaatat tggaacagga acttcaataa tagtacttgg acattcgaat ttacaacaaa
     2461 tcttattgtt atttgcttgg tatagcttc
//
LOCUS       DAYYBG010000179         2478 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000179 DAYYBG010000000
VERSION     DAYYBG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.187"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..903
                     /locus_tag="ACHAUN_002856"
     CDS             <1..903
                     /locus_tag="ACHAUN_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8461480.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..1824
                     /locus_tag="ACHAUN_002857"
     CDS             916..1824
                     /locus_tag="ACHAUN_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8461481.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1824..2444
                     /locus_tag="ACHAUN_002858"
     CDS             1824..2444
                     /locus_tag="ACHAUN_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461482.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          843 a          365 c          582 g          688 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagatgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataagaata cagaggttat ggaagcgact
     1021 atagttgaaa atggtcttcc ctacgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gtaggtggga aatatccagt ccaattagaa acaaaaattg tggatgctaa aaaagggaaa
     1141 attaactata tttttgataa atattccatg cagtgtttac atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctctta ttttgtcatt
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat cgaagattta
     1321 attgcggata tgacggactt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga acgttggaga caagaacagg aacacagttt ccaagattgg
     1441 agagaaggac aagaatcaga ctatttggca tggtttgaat caatcaagga tattttaaag
     1501 acgattgatc caggtggagt catgttagcc gaactaacgg atgcgcgagt agatattcaa
     1561 ggagttcgtc atgcgtctat ttcagagcgt ttgctggcag atatggacta tttatatcag
     1621 aaattgcgag caacgctttt cacgattgaa tacggggaga ttgaagtgac tgatattttg
     1681 caggatgatc tcttttcaga taatcatgaa gtcgaaaaag ttggaactgt agcattcccg
     1741 attgaagaag gagccttgat catcgcaacc gttgatgatc caaagcaaaa tgtgttcact
     1801 cttgagaaag ttggggtgat ctaatggcta aaactaaacg aatgatggaa acggatgaaa
     1861 aaacgggtgt acaacgccaa ttctttccaa tcacacatgc ttccgcagtt cttgggttag
     1921 aagaaataat agcaggagaa gcaacagttt tatctgttaa tggaaaaatt ggagccgtcg
     1981 tcattactaa agaagatttg ggattagaga atgttctcac agaattaccc tatgcaagtg
     2041 aagaagatga cggcattatc acggcagaaa tgtatcaaaa aattttaaac agtggagaag
     2101 gtgactacgt gttgccagtt gccactatcg accgtttggg tggcataaaa gttggtgaat
     2161 tattgacgat tgatgagaca gggaaagtct ctgcagtcag acaatctgat gtcaattttt
     2221 cgctggagtt aaaagaaaaa ctcgattcac tgaaaaatta tactgctgga gaaaacatca
     2281 ctattgatga agatggaaca attagttcta caggtggtag tgggactggt ggtgtcaatc
     2341 agtcatatgt agatcaaaaa ttccaagaag ctgtaaatca agcagaaaat tacacaaacg
     2401 aaagaattcc aaattttact tttgaaaaaa ttggggaggt atagagaatg acagatatcg
     2461 taaaaataaa acaaagca
//
LOCUS       DAYYBG010000180         2458 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000180 DAYYBG010000000
VERSION     DAYYBG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.188"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACHAUN_002859"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACHAUN_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACHAUN_002860"
     CDS             1320..1778
                     /locus_tag="ACHAUN_002860"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8461483.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="ACHAUN_002861"
     CDS             1937..2407
                     /locus_tag="ACHAUN_002861"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8461484.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          459 g          716 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctt
//
LOCUS       DAYYBG010000181         2400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000181 DAYYBG010000000
VERSION     DAYYBG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.189"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHAUN_002862"
     CDS             207..2069
                     /locus_tag="ACHAUN_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8461485.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACHAUN_002863"
     CDS             2069..>2400
                     /locus_tag="ACHAUN_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8461486.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAYYBG010000182         2395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000182 DAYYBG010000000
VERSION     DAYYBG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.190"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHAUN_002864"
     CDS             316..570
                     /locus_tag="ACHAUN_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8461487.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHAUN_002865"
     CDS             571..888
                     /locus_tag="ACHAUN_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8461488.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHAUN_002866"
     CDS             1447..2382
                     /locus_tag="ACHAUN_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461489.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAYYBG010000183         2354 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000183 DAYYBG010000000
VERSION     DAYYBG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.191"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..2207
                     /locus_tag="ACHAUN_002867"
     CDS             54..2207
                     /locus_tag="ACHAUN_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8461490.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          809 a          416 c          528 g          601 t
ORIGIN      
        1 agaagttttt gggtacaatg aagcatgaat gtaaataggc ggaaggatag gaaatgatga
       61 aagattttat gaaaaaaata ttaaatactt cttggttaga aaaaataaga aaaggtgcac
      121 aagaacctaa gaacaaaacg tttcgccggt cccatgttcc ttttcgctta aattttcttt
      181 ttttcattat atttacgtta tttgttgcgt tgattgctag attgggttat ttgcagatcg
      241 tgaacggcga agagatggag gcgcgagtga agtctacttc tacgattacg gtccaagaaa
      301 gtactcctcg cggaatgatc tacgactcca caggaaaagc tttagttacg aataaagcga
      361 atcaggccat cacatatacg agaggaacac aagttacggc taaagatttg cttgaaatcg
      421 caacgaagct gaactcgctg atcgatgttc cggtagatga aaatctgaca gatagagata
      481 aaaaagattt ttggctggct gatcccaaac atttgaaaga agccacaaaa agactttcgg
      541 caaaagaaaa gcaattaggg aacagcgaac aatatagtgc gatggtcaat aaagtcacag
      601 ctgatgagat taatttaaac gaggagcaat taaaaattgc taccatcttt aaacggatga
      661 atagtgcata tgaattaaac actgtctata tcaaaaactc tggtgttact gatcaagagt
      721 tagctgttgt ggcagaacgc gcttctgaac ttccaggagt ttcgactggc acagactgga
      781 cacgagaata taatgcagca agttcactga aaagtattct tggcagtgtg acgactgaaa
      841 agcaaggact tccagcagat gaagcagaga aatacctagc aaaaggttat tctcggaatg
      901 accgtgtagg acaaagttat ttggaaaaac aatatgaaga tgtcctgcaa ggaaccaaaa
      961 cccaatatga agtttccctt gataatgaag gaaatgtttc gaatcaaaaa gaaatcttct
     1021 ctggggaaaa aggttctaat ttaatgttat ctatgaatgc agaattccaa tcaaaagtag
     1081 aagaaatttt gaaaaggaat tatcaaacat tgatcaacaa cggcaaagca cagtattcgc
     1141 ctggtgctta tgcagtggct atgaatcctc agacaggaga agtgctagca atgactggtt
     1201 tcagtcatga acaaggttcc aaagaaatca ccgagaatgc tttaggcacg attactagcg
     1261 cctttgatcc agggtctgta gtcaaggctg gtactttgac agcaggctgg gcaagtaacg
     1321 cgatcagcgg aaatcaagtt ttgattgatg agccgattcg tctgcaaggt gcttcagaaa
     1381 aaagctctgt ctttaatcga agcggacaag ttgctcttga tgcagtcaaa gcatcggaat
     1441 tgtcttctaa tacgtatatg atcaaagtgg cgttaaagat gttaggacta gactatacac
     1501 ctggaatggg actacctagt ttagatgaag aagctaaagc ttatcagcaa ttgagagact
     1561 ctttcaaaga gtttggtctt ggtacaacga ctgggatcga tttgccgaat gaatctcctg
     1621 ggatctcacg ttctgttgat tatatgaaga agttcaatgc ggataatggc aaagaatggt
     1681 atgctccagg taactttacc gatttggcat ttggacagtt tgacacatat acaccaatcc
     1741 aattagctca atatgcttca acagtcgcta atggcgggaa acgagtacag ccgcacctag
     1801 tcaaagcgat ctatggcaat gacgaaaatg gaaacttggg tgaagtgaag aaagaaattg
     1861 gcacaacagt agaaaatacg gtcaatattt ctgcagaaaa tatggcgatc ctccgtgaag
     1921 gattccacca agttgtccat ggtaccgatc cttacacaac agccaaacca ttagccagtg
     1981 cgaaaatgga cctttccgca aaaacaggga ctgcagaaac ggttgcggaa ggtcatccag
     2041 atattgcgac agtcaatagt aatatcgtgg catacggacc aacagacaat ccggaaattg
     2101 ccatcagtgt tgtcttgccg aacttattag acgaacaaga ccacataaac ttgacgattg
     2161 ctaaagaaat aatggacgcg tattatgata tgttcatggc aaaataaaaa agaggtcgtg
     2221 aaaaagccgc ttagtttcga gtatgaagaa aaattttgtg aaaatagctt ctcacatttt
     2281 tgcttaaatt tgacttcata ccgagaaact ggcttttgaa caccgtttac tcggcaaaaa
     2341 aggttgtgac aaaa
//
LOCUS       DAYYBG010000184         2344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000184 DAYYBG010000000
VERSION     DAYYBG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.192"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..437)
                     /locus_tag="ACHAUN_002868"
     CDS             complement(111..437)
                     /locus_tag="ACHAUN_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461491.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(483..845)
                     /locus_tag="ACHAUN_002869"
     CDS             complement(483..845)
                     /locus_tag="ACHAUN_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8461492.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(882..1096)
                     /locus_tag="ACHAUN_002870"
                     /pseudo
     CDS             complement(882..1096)
                     /locus_tag="ACHAUN_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1224..1559
                     /locus_tag="ACHAUN_002871"
     CDS             1224..1559
                     /locus_tag="ACHAUN_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8461493.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1635..>2344)
                     /locus_tag="ACHAUN_002872"
     CDS             complement(1635..>2344)
                     /locus_tag="ACHAUN_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461494.1"
                     /translation="TFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNFNHKRSNVYRL
                     DVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSK
                     EKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVCERLRNVGNNSVENALKENRGYFVK
                     QGFTLERASKDIKRLYNVKASSSFLVYLAKGAGIDEVIKELILSGVELVPLYQFTSVT
                     RDDGRRVRKRKDPIVLN"
BASE COUNT          717 a          474 c          324 g          829 t
ORIGIN      
        1 tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact ccctagtgaa
       61 tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa tcaatttaac
      121 attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg atagtactca
      181 atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta tgtgttatct
      241 ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta ctgttcttgt
      301 aaaatctgtg ttaccaatgg tttcgatgac ttctctaatc cagatgtacc tgtcattttt
      361 gaaaaaatga tacctataat agccaagaca acgataacta taatgataaa cttcttgttc
      421 atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc ttatccctct
      481 tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac ccctgctatc
      541 atcaaaatcg tacccatttg ttcatcttta tctcgatata agtagcccat aataacagca
      601 acagcaccaa atacaacagg aataaatgct aacgctacta ccatagaaat ccaaccaata
      661 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
      721 gtctgctcag tacttttctt tactgcgtct gctccagttg tctcatctgt ttcttgtagt
      781 gctccgcaaa actggcaata cttactttca tctgttattt ctttgccaca ttttctacaa
      841 tacatgtctt ctcctcctaa aaacattcaa ttgtttatta gttaactgtt tactttatta
      901 aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag catcttcatt
      961 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
     1021 ttcatttttt tgctaactcc gtttgagaaa gtcctttttc ttttctgtat cggctgagat
     1081 tacttacttt tttcatgatt ctcccctcct gttttattat atacaacaat atgatgtatt
     1141 tcaactcata aaatataaaa aaacttcaaa taccattaca aatacaacaa cttgttgtac
     1201 tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc attagagaaa
     1261 agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc tctacacaac
     1321 aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc ttaaccgctc
     1381 tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc gtagattatg
     1441 aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt cgaacaattg
     1501 actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg aaagattgaa
     1561 aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa gattaagagc
     1621 tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac tctccgtcca
     1681 tcatctcttg ttacacttgt aaattgataa agaggaacta attcaactcc tgataatatc
     1741 aattctttta ttacttcatc tatccctgct ccttttgcta ggtagactaa aaaactacta
     1801 cttgctttca cattgtatag tctcttaata tccttagaag ctcgttcaag agtgaatcct
     1861 tgtttaacaa agtagcctct gttttctttt aaagcatttt ccacgctgtt attcccaaca
     1921 ttacgtagac gttcacaaac agtgttagta agttctttct ctgtcattag atatgttcct
     1981 tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg tttttccttg
     2041 ctaagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc gttaaaacat
     2101 atcttaaccc attcataaga aaaatattga tatgtaaaat gaaatttttc cagaagtgga
     2161 aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc taatcgatag
     2221 acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa cctatttagg
     2281 tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa tcctataaaa
     2341 gtca
//
LOCUS       DAYYBG010000185         2304 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000185 DAYYBG010000000
VERSION     DAYYBG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.193"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1583
                     /locus_tag="ACHAUN_002873"
     CDS             51..1583
                     /locus_tag="ACHAUN_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8461495.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            1761..1982
                     /locus_tag="ACHAUN_002874"
     CDS             1761..1982
                     /locus_tag="ACHAUN_002874"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8461496.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          782 a          240 c          438 g          844 t
ORIGIN      
        1 tatatagtat taggtaacaa taaaaaatag aaagtttgga gccatatgat atgataatta
       61 atcgtaaaca aaatgctaaa aggaatataa ttactggaac aatttttaag ttatatcaga
      121 ttatcattcc cttttttatg cgtacactta tgatttatca gatgggaatg gagtatgctg
      181 gattgaacaa cctgttcaca tctatatttc aaatgttaaa tttagcggaa ttgggaatag
      241 gtgctgcact aactgtaagt atgtataaac ctatagcaga agacaataga aaagagattt
      301 gtgctttact gaacgcatat aaaaagtact tttcaattat tggatggata gtgtttacat
      361 taggattgtt atgcattcct tttttgagat ttttagttaa aggttcgatt cctggaaatt
      421 taaatatgac agttttgtat ttaatgtatt tgataaatac tgttttgtcg tattggttat
      481 tttcatatag aaagagtcta atatatgcac atcaaagaaa tgatgtaatt aacgtgtcta
      541 tgctaattac ttttacattc caatttgtaa tgcaggcata tgttctgatt ttcttaaaaa
      601 attactattt gtatttattg gcttctattt tgggacaaat agttcttaac ttaatgtgtg
      661 ctgttgtttc tcaaaagatg tatcctgatt atactcctgt tggtgagtta gagaaagaaa
      721 ctattgacga tattataaaa aaagtaaaag gattagttac aaacaaggta ggcggtacaa
      781 tattacgatc ttcagattca attattattt catctttctt aggtctttca gtacttgcga
      841 tttatcagaa ttattacttt attatttctg ccataatgag tatatttgca ataatttatg
      901 aatcatgtat agcaggtata ggtaacagtc ttattgtaga aagtcaagaa aaaaattata
      961 tagatttttg tgatattaca acaattatag gttggtttct ggcaattagt tgtgcatgct
     1021 ttattacatt atttcagccg ttcatgatta tctgggtggg aagtgataat cttatgagta
     1081 tttcatttgt tatatttatg atagttcatt ttttcttata tgaaattgat cagttaattg
     1141 ggttatacaa agatgctgct ggaatatggt acgcagacag gtttagacca ttaattacag
     1201 caatgataaa cattgtatta aatatcatcc tcgttaattg gttcggatta tatggagttt
     1261 tggcggcaac cattgtttca gtattgttgg ttgatttacc ctggttatta tataatgttt
     1321 tccaaaatat atttaaattt gaatcggcag gtaaatatgt ttggaagctc ataagtttgt
     1381 gtgtaatagc ttttgttgct tcttatttaa gttattttgc ttgttcgtat gttaatatcg
     1441 gaaatttcaa agtgataagt ttagtgataa atttagttat atcggttgtc atatcgattt
     1501 tagttattct tctgtttaat atccgtaata agaagtttaa gtatattttg aaagttctta
     1561 cgtcggtatt ggtaaaaaga tgagaagaga catcagaatg atatataaag taactacaat
     1621 ttttaggctg ccgaataggc gaacgatgta tagtttttgt tccaacgaag actcttgttg
     1681 aaattggaga tgatatgaag gagttacaat tttgactcat gtttatgatt gatcggtttt
     1741 aaaagtagta tggagaagct ttgggatcat cggagaggat caaaataggt aataatgttt
     1801 ttataggaat gaagtctaga atactaaagg gtgtacatat aggaaataat gtagttatag
     1861 gtgcaaatag tttagtaaac aaggacgtat cagacgattg tgttgttgca ggaaatctag
     1921 caaaagtaat tatgtcccta gatgaatatt ataaaaagat aaacaatatg aagaatccgt
     1981 aaaacttgtg aaactatacc gagaataata tgggaaaaat attgatgaaa aagcattata
     2041 tgagttttta gggctgttta cagatggaga tgacaatttg tcgaagagtt gggagcaatg
     2101 caaaatttag taggggatta taaacagact gacaaggtat ttaaagaaca tgcacaagta
     2161 tttcataata ttgaagaatt tttgaatact gtgagatagg agttgctaca ttgagaaaag
     2221 tatgtatttt tccaatgtaa aagattacga gtttgaaaac gacaccataa tattattttt
     2281 taagaagata ttatagagct tagg
//
LOCUS       DAYYBG010000186         2296 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000186 DAYYBG010000000
VERSION     DAYYBG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.194"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..462
                     /locus_tag="ACHAUN_002875"
     CDS             <1..462
                     /locus_tag="ACHAUN_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8461497.1"
                     /translation="YYDEQYIYQAVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYN
                     GRFTIRQTETFEIYNPEKYSSKPLIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLE
                     EAYRKLDGNLEHQDQVTLFLDFPFLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            459..>2296
                     /locus_tag="ACHAUN_002876"
     CDS             459..>2296
                     /locus_tag="ACHAUN_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8461498.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          858 a          279 c          448 g          711 t
ORIGIN      
        1 tactatgatg aacaatatat ttatcaagct gtagtaatag aaggacctga atttaaagga
       61 acaagaaaaa caggaaatat tgtccctttt gaatttacag tgagtattcg accatttaag
      121 gaaaattaca atggtagatt tactattcga caaacagaaa cttttgaaat ttataaccca
      181 gagaagtatt cttcaaaacc gcttattaaa ttgagtggtt ctggagatgc ttctttttat
      241 attaataagg ataaatatga tttgaaatcg ttggacagag aattatttat agattctaaa
      301 ttagaagagg cttatcgaaa attagatggt aatttagaac atcaggatca ggtcacctta
      361 tttttagatt ttccattttt atatcctgga aaaaatgaaa ttaaatggac gaaaaatatt
      421 cattcatttg aaataatgcc taggtggtgg agaaaagtat gaaaccaaga atatatagtc
      481 ctactgaaac agatttctca acgaatggct taggaatttt aaaggattgt acaagatgtg
      541 gaatatatga agtagctaat ggaaaatatg aattagagtt ggattatcct ttaggaacta
      601 gatttgatga atattttgaa aatgactatc aaataaaagc aaagccaaac gatcaagaag
      661 agtatcatat cttttttatt gatgataaag atatagatac ttttttaaat acagtaacta
      721 tttatgctca gagtcgtaca aatcgacttg gaagacgggt agtcactctt gtggaagtgg
      781 actctaaaac tggtcaagaa gcgatatcaa ttattgaaac taaaatggat aaaaaatctg
      841 acatacgact ttattctgat attacggctg tttctagcac aatctttgaa gcgagaaatg
      901 ttttaaattg tattgctggt gaacaaggat cattgcttca atattggggc ggagaaatta
      961 aacgtgaacc atttaagctt tctttgttaa agcgaagagg tcgcaataat gttggaacga
     1021 ttcggtatgg aaaagatatg tctggtttga aggtcaaatt agattggaca ggtataaaaa
     1081 caagaattat tccatatgct gatcctcaaa gtgaagtagg tacgactaat cgaatttatg
     1141 gttcgccagt agatagtgcc tatattaata actatcctga tgtctataca gaacatgttc
     1201 agtttacaga agaacaagga gtaaaagaca ttaagagctt aaataaaata gctaaaaatt
     1261 actttaaaac gatcaatcct ggctgtgata agcctaaagt ttctattacg gttgaatttg
     1321 ataagttgac tgatagtgaa gaagcgaaag aatttgcgaa gattagaaat tatggtctgt
     1381 ttgatacatt taaaatatat cataaaaaat ataatattta tcttgaatca aaggttagtg
     1441 gactacaata cgattcttta tctgaaaaaa ctttgaaatt agaagcagga gacattcaag
     1501 ttgcttttta tcagcaacaa gctgtaacta ttcaggataa attaaaagat tatgcaacga
     1561 ataattatat gagtgatttt aatgattatg tttcttcaat gattacaggt caaggcagtg
     1621 caggaggata tgtagtttta tggccaaaag aaaaaccttc caacatcttt attatggata
     1681 atccagattt agaaaaggca agagaagtac taagaatgaa taaaaatggg attgcttttt
     1741 ctaagaaagg atggcggggg ccttttaatt cagcttggac attagatagt atatttaatg
     1801 ccaattttat acaaactgga ttaatcaaag cagatatctt tcaaaattct tttaacaaga
     1861 ctggagatgt attgaaatta gttaatggac tactccaaat ttggaacaac aaaaagaaaa
     1921 taatggagtt aactaaaaaa ggaatggaat tttggaattc taagggttca attggaacga
     1981 ttggaacaac tgattctgtt ggtaatcctt ttcctggggc ttctactcca acccctattg
     2041 aagataattc tttagttatt cgtacaaatg gagacggcaa atatattttg atttctccta
     2101 aagtaggaaa aggattagtt ttattaggaa atggtaaagc aatttatttt ggagacttag
     2161 atgtacaagg aaaacttaca gttaaaggaa aagaaataat tcctggacaa ggtggtggtc
     2221 catcgggagg tggagaatct actggtgggt atcctagtga attaaaaaca gatgcagaaa
     2281 agagagcttg gagaat
//
LOCUS       DAYYBG010000187         2290 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000187 DAYYBG010000000
VERSION     DAYYBG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.195"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHAUN_002877"
     CDS             278..820
                     /locus_tag="ACHAUN_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461499.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ACHAUN_002878"
     CDS             976..1425
                     /locus_tag="ACHAUN_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8461500.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>2290)
                     /locus_tag="ACHAUN_002879"
     CDS             complement(1455..>2290)
                     /locus_tag="ACHAUN_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFM8461501.1"
                     /translation="CKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKL
                     KKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          795 a          376 c          434 g          685 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc cccggtctgt atgaaaaagc
     1801 ttgacgtctg ttaaagaata cggtatccgt ccaaaggctt cttttaccaa tgaggcatct
     1861 ttcttctcac cacaggaata accaataatt tctcgattaa acaaatcaag tattaagcag
     1921 atataatgcc actttttccc cacgcgaaca taagtaagat ccgtaacgat ggcttccaat
     1981 ggctgttctt gtgtaaaggt ccgatctaat acattcgctg ttttcgcttc attacaagct
     2041 gttcgttgcc ctttaaaatg agcgatcgta taggtagatg tcaatccgcg gcgtttcatg
     2101 attcgaccga ttcggcgccg actgagttga agcccacgct ttgctaaaca cttcttcaat
     2161 tttcgggtac cgtaagcctt tcggtttcga ataaactctt cctgaacgat ctcttctaag
     2221 tcggactcat tttcgatcgg tttcgcttga taatagtagg tctgacgaga aatatttaga
     2281 attttgcaca
//
LOCUS       DAYYBG010000188         2285 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000188 DAYYBG010000000
VERSION     DAYYBG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.196"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..530)
                     /locus_tag="ACHAUN_002880"
     CDS             complement(162..530)
                     /locus_tag="ACHAUN_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8461502.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            680..1162
                     /locus_tag="ACHAUN_002881"
     CDS             680..1162
                     /locus_tag="ACHAUN_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8461503.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1199..2209
                     /locus_tag="ACHAUN_002882"
     CDS             1199..2209
                     /locus_tag="ACHAUN_002882"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8461504.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          801 a          354 c          461 g          669 t
ORIGIN      
        1 cagtgatttt tttcagcaca agaaagactc cttataaaaa tagcagatac tttgtcaatt
       61 aagtgtttaa ccagggatga taaaaaggat aggattgaac aaaacctgta taaggtgatg
      121 aatagtttcc tcgccagtaa aaggtttatt cgtataaagc tttatcgcca aaatttatca
      181 tgaccttttt tatcttcaat attaaatgaa atcaattgtt caatcaattc ccagttgatt
      241 acacttttcc aaggaacttt gatgacgtta tccgtatata cgtagcctgc ttcttggata
      301 tttttttcaa gggcacgaat cgctgctttt tccggtgcga tagaaaaatg atttttagca
      361 aaactaaatg caataataaa ggtaccatgg tcagtaaaca taggttggtt ccattttaca
      421 gttgtgtcta gctggggata cgtttctgtt atcttagcaa aaaggtcttt gatcttttgt
      481 tgatgttctt tttctttgat ttgattgatg atcggctgaa atttttccat tctaaaacgc
      541 ctccattaag tggtttgatt tattttacca gaaaaacaat agttatgtat aaaaaacgaa
      601 caaaacacaa gtaatgttgt aaccgctata tttatatgat aagatgtctc aaataataaa
      661 atagaaaaga gacataaaaa tgattgactt ggcaaagaaa ttcttacacg aaaatatata
      721 ttttgagttg tatcatcatc gagccattta tacaaatgaa gatgctttgg taataaagaa
      781 agaacaagga tttacaggta cagagaccaa aagcttattc ttaaaagata aaaaagataa
      841 taactacgta tatctgactt ttactacgaa acaaacggat ttcaaagcat tagggaagat
      901 cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg gaagaacaaa cgggacaaaa
      961 gcagggcgct gtttctccgt ttggttatga aaaacagatt ccgattatta ttgatgaaga
     1021 gctcttatca aatgaaaaac ttgtttttgc accaggtaga ccagatcaaa cgatggtcat
     1081 ccaaacggtt gacttaaaca aaatcatcca gctacttgag atgccgatat ttttgatgcc
     1141 ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat acttggaggt gaagcaacat
     1201 gataagttat ccattattag aaagaaatgc aacgattggg gttactgcac catcctcagg
     1261 cgtatcagag gaacaccatt cgttactcga atcagcttta aagagaatgg aagaaaaatc
     1321 tttccaagta gttactggaa agacgctttg gacgcaagat aaggaaaaat caggtccagc
     1381 aagaaaacga gcagatgaat tcaatcaaat gatgcaagat gaaaatatcc aaatgatctt
     1441 tccgacttgg ggaggagaat tattgattga gattctcgat tttgtcgatt ttgatgctct
     1501 acaaccaaaa tggatagtag gttattcaga tattagtacg ttgctacttg ctgtcacatt
     1561 aaaaacaggg atagccactg cacatggaac taaccttatt gatttaagag gagagtactt
     1621 ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca agagaaggag aatcgattct
     1681 acagcaatct tctgaaaagt atcaaaaagt gtggccccat gagcattcat catcttctgt
     1741 tttccatctc actgaaccaa caaattggca atcagtcagc ggagatgcag taaggatgaa
     1801 gggacgatta ttaggtggtt gtattgatac gatcaggcat ttaatcggta ctccttatgg
     1861 agatgtccaa tcttttaaaa gaaagtataa tgatggaaag ccgatcttgt ggtttctaga
     1921 aaactgtgag ctatcgtcag ttgatttaag aagatcactt gttcagatga aactggcagg
     1981 ctggtttgat ggcagctcgg ggatcatgtt cggtagaagt gcggcagagg atttgttaga
     2041 ttataaaatg aaggatgtat acgaagatct gtcaaaggaa ttaaaactac cgatcttgta
     2101 tgatattgat tgtggtcatg taccgccaca actgacattg attaatggtg cctatgcaga
     2161 agtagcggta gaagatagaa aagcgactgt tttgcaaacg tttatttaaa aaattttttc
     2221 ctgccttgga gtctatcaaa gaccaataaa tctaaataga aaagaaagtg aaagaagctg
     2281 acaaa
//
LOCUS       DAYYBG010000189         2282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000189 DAYYBG010000000
VERSION     DAYYBG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.197"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            854..2023
                     /locus_tag="ACHAUN_002883"
     CDS             854..2023
                     /locus_tag="ACHAUN_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8461505.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          830 a          315 c          334 g          803 t
ORIGIN      
        1 aattttctac gttttccttt tcatatatat taaatattac ggaaaattat ataaactaat
       61 ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt taggtctatt
      121 taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc tttcgtactt
      181 aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac tagtatgata
      241 tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt cctaaaacct
      301 ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa catgaaatta
      361 tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa catttattga
      421 ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt gataataaag
      481 tactacctat taaaggtagt actttattta atttagtata aattaacata ctactatatt
      541 ttaggtttca tattatataa attaataata tactaccatt tgttggtagt ataatttaaa
      601 ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa gtttaataag
      661 agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct ttagtgatat
      721 aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa tagatattta
      781 gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg aaattttata
      841 ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa tcaataaagg
      901 tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat tattgcaggc
      961 tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca attcaggtaa
     1021 ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat taaatttggt
     1081 aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt ataaaagaac
     1141 caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta ctatgtctga
     1201 aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa ccatttcaaa
     1261 tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct tagaatccag
     1321 atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa ctgtttcaaa
     1381 agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg ttcttgggtt
     1441 tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag acctaaaaga
     1501 ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca ttaacgatag
     1561 tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata tatctagaaa
     1621 tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt tcaaattagt
     1681 ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga tagataaatg
     1741 gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat taacctttta
     1801 taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg aaggttttaa
     1861 caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag aatctctgga
     1921 gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc gagttcataa
     1981 aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc aacagaatgg
     2041 gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct tgcgtaaagt
     2101 tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg ttattcatat
     2161 gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa acttaaattt
     2221 catataattt aattatgtaa attagctatt ctaagaactt ctacggataa ctgcccgtct
     2281 aa
//
LOCUS       DAYYBG010000190         2265 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000190 DAYYBG010000000
VERSION     DAYYBG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.198"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1125
                     /locus_tag="ACHAUN_002884"
     CDS             <1..1125
                     /locus_tag="ACHAUN_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8461506.1"
                     /translation="SSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTE
                     GQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDL
                     YASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQS
                     KFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQY
                     YLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAA
                     TQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQI
                     IGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="ACHAUN_002885"
     CDS             1474..2106
                     /locus_tag="ACHAUN_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8461507.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          844 a          308 c          454 g          659 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaac
//
LOCUS       DAYYBG010000191         2221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000191 DAYYBG010000000
VERSION     DAYYBG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.199"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2139
                     /gene="acm"
                     /locus_tag="ACHAUN_002886"
     CDS             <1..2139
                     /gene="acm"
                     /locus_tag="ACHAUN_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8461508.1"
                     /translation="SMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGN
                     IKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSA
                     QVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGS
                     SSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQ
                     IHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTII
                     ENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYIND
                     TEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWID
                     FDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRAS
                     TNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQK
                     IENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDD
                     WRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITP
                     WKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKT
                     SSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          876 a          354 c          423 g          568 t
ORIGIN      
        1 tcaatgttat ttattattac taatataaca tcactgattc ctgttcatgt ctatgcggat
       61 gcaggcagag atatcagcag taatgtcact tcgttgaccg tggacccaac gaatattaca
      121 gatggaggaa acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca
      181 ggtgattatc tttggatcaa ttggccaagt gaaggaaata ttcgcggtga aggattccaa
      241 aaagaaatcc cattaatgat tgaaaataaa aatgttggaa ctttaacggt cagaaaagat
      301 tctgcgcagg ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga aggttggggg
      361 caatttgaga tacaggccag aaacgtaacc gataccggtg aagaaaatac gggaccattc
      421 accgtgacga gcggtgataa aacagctact gtaaatgtga caaagccagc ttctggttct
      481 tcttctagcg ttttttatta taaaacagga gacatgctac ctgaagatac taagcacatt
      541 cgatggtttt tgaatattaa caataacggc acttatgttg aacaaccagt taaaatcagt
      601 gatgaaatcc aaagtggaca aagattagat ccaagtacgt ttgaaattaa tcaaatacac
      661 ttaggtgaac agaaggttta tagaggagaa gagggaatcc aacaattttt acaagatttc
      721 cccagcgcta catttaattt ttcagtaact gataattaca ttgaaataac tataccaaaa
      781 aactttgtca atcttaggaa aataatggtg agttataaga cgatcataga aaacccagaa
      841 caaataaatt ttgagaatca ttcggaagct tggtttaaag aatttaataa acctgctgtt
      901 gatggagaaa gtttcaatca tacagttaaa aatatttcag cttctggtgg agtcaacggt
      961 acagttagag gcgaattgaa gattttcaaa tatatcaatg acacagagat tggtatccca
     1021 aatgtgactt tcgaattaag acgagcagat gaacagccta ttcaaggaca gtctagtatc
     1081 ctgttgactt cgaatgaaca gggagaagct agtatcaaag gcctgcaagt aggagactat
     1141 gttgtaaaag aaaaagaagc acccaattgg attgattttg atccactaag ttcaaatgaa
     1201 ctcaaattct ccataaacga aaatgataca gagggagtca gtttacctat ttataacaag
     1261 aaaaaggtta cgaacatcac agcaactaaa atttggaacg gtggaaccac tccaagaccg
     1321 agtatttatt ttaaattatt cagagcaagt acaaacaatt gggaaccagt tcctgatgca
     1381 gaaacaaaac gattagacaa tggaataact tcagtgactt gggaagatat ccaacagtat
     1441 gatgatagtg gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat
     1501 tatgtccctt caggttatca aaaaattgaa aacgggctag tcgttacaaa tgagaataaa
     1561 gaagtcattt cagtctcagg acaaaaaaca tgggaagaca atgagaacca agacggcaaa
     1621 cgaccgacga cgatcacagt aaatctatta gccgacggtc aaccgatcca acacaaagaa
     1681 gtaagtgaaa aagatgattg gcgctatcgt ttcacaaatc tgccaaaata cagagacggg
     1741 caagaaatca tctatactgt aacggaagac aacgtgcccg aatacagtac gacgattgac
     1801 gggtataaca tcaaaaacag ttatacaccg gaaaaaacaa atatatctat accaggtagc
     1861 catataacac cgtggaaaaa ttttccagag aagacagaga gagagaaaat tagaaatcta
     1921 ttctctcaaa aattgcaaat atttgctcat acaggagaat ccagcgaaca aagaagcaat
     1981 tatattagta agagagctgt acctaaacag ataacaaata aatcaaaaag cattcttcct
     2041 aaaacaagta gtaaaaaatc tagttttgct gtaatcattg gtttattgat agtaactatc
     2101 tgtgctggga tattcttaca aaaatataaa aaagtttagt tagaatataa tattttttgt
     2161 taaatatgac tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt
     2221 t
//
LOCUS       DAYYBG010000192         2206 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000192 DAYYBG010000000
VERSION     DAYYBG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.200"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHAUN_002887"
     CDS             complement(<1..914)
                     /locus_tag="ACHAUN_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8461509.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACHAUN_002888"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACHAUN_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8461510.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACHAUN_002889"
     CDS             2006..2176
                     /locus_tag="ACHAUN_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461511.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          748 a          384 c          421 g          653 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataa
//
LOCUS       DAYYBG010000193         2022 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000193 DAYYBG010000000
VERSION     DAYYBG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.201"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            635..967
                     /gene="traE"
                     /locus_tag="ACHAUN_002890"
     CDS             635..967
                     /gene="traE"
                     /locus_tag="ACHAUN_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8461512.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1586..1927
                     /locus_tag="ACHAUN_002891"
     CDS             1586..1927
                     /locus_tag="ACHAUN_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8461513.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          702 a          271 c          428 g          621 t
ORIGIN      
        1 caaatataga aaagggaacg tatgtttgca tactcctttt ttttgagtta taataatacg
       61 cgaagagaga taatttcatt atttaaacaa gctaaaaatc ttgatattac ttcttttcag
      121 gcaatacgaa tattattcgc ttacattttt tgtataaatt taattgtctg ctatgcttgt
      181 gatgaattga ttttcgtttt gcacttttgg atgataaatt tggacaattg aaaatacaaa
      241 aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt catacacggc
      301 attaagattt ggcccttaat gcgttaacct ttcagtgtta cggtattacc cgcaatggat
      361 ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa ggttaaaaat
      421 acgtgatatt acgatgaatc tatgcctaag cataatgtta taaaaatgtc cgtgtggctc
      481 tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttggatgcg ttccctcttt
      541 tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag aaaaacttgt
      601 taaaaatatt taaagaaatt gggtggttta ttttatgtca tatacaggaa ttttgagtct
      661 caaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca cgaagaaact
      721 atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa
      781 agtctcagag gaaatgattt attacattgg gaaacaaaag caaataattt taaaagatcc
      841 tatcccctta aaagagctat atccaacaat caaacatgtt tatgaccaaa acggtgtact
      901 gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg ggcgtttgat
      961 cggctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat
     1021 tgtcgagact tctaaaggaa cttagagtca ctagaaaaat aaggagtaga cttgatctat
     1081 caacaattcc cttcggggag tgacggggta ggttttaacg attggtaacg taaaaaattg
     1141 gtcaatcgag taaatgcgct tgtttggata gtggtgaaat gaatttgtgt aaataataag
     1201 cgcagagtaa acaagaggga atttagaagt tagacaagtt agaatagcgt ctagattaat
     1261 catgagcagc tactaaggga tctagacttt gacgcaaact catggaataa ggagtaggtc
     1321 ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac
     1381 ttgcgaggac gttgattatt ggatcaatcg agcatttacc gctgtctact agggtttctc
     1441 gtttcggacg ccataaacga gtcgaaattt tttatgtatt tttaagtaaa cgaaaaacga
     1501 taaaatatga acaagtcctg aataaagtga aaaaattaat ataaagcata gagaaaatga
     1561 aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac gattgggcac
     1621 acatactatt aaggtttctt tccaacgttg ggattacaaa ggatttgtga cgtttagaag
     1681 gggcggaaat tgtaaaggat tagatgtttt agcattagac gaggatgatt tatacgatca
     1741 aacattgact gataatccaa ttggattcgg cttgctacct gaagatgatg aaggtaatga
     1801 atggtttaaa atgacgctta tgaatgataa tggagacgaa ttatctgttg aagatacttg
     1861 gtcatatttg agtgattata ttgtcagctt tgaaatcatt gaatttgtag cggataaaga
     1921 agaataaaaa ctggaggaat aaattttaca gataaaggct tagaaggagt cacgacgatt
     1981 aataaaaagg tagtattacc ttaggcattg agtaaggaat tt
//
LOCUS       DAYYBG010000194         2019 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000194 DAYYBG010000000
VERSION     DAYYBG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.202"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..570
                     /locus_tag="ACHAUN_002892"
     CDS             100..570
                     /locus_tag="ACHAUN_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8461514.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="ACHAUN_002893"
     CDS             792..1895
                     /locus_tag="ACHAUN_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8461515.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DAYYBG010000195         2003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000195 DAYYBG010000000
VERSION     DAYYBG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.203"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACHAUN_002894"
     CDS             <1..378
                     /locus_tag="ACHAUN_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8461516.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACHAUN_002895"
     CDS             426..1826
                     /locus_tag="ACHAUN_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8461517.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAYYBG010000196         1979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000196 DAYYBG010000000
VERSION     DAYYBG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.204"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACHAUN_002896"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACHAUN_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACHAUN_002897"
     CDS             complement(728..1744)
                     /locus_tag="ACHAUN_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8461518.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAYYBG010000197         1901 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000197 DAYYBG010000000
VERSION     DAYYBG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.205"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..913
                     /locus_tag="ACHAUN_002898"
     CDS             5..913
                     /locus_tag="ACHAUN_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8461519.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            913..1530
                     /locus_tag="ACHAUN_002899"
     CDS             913..1530
                     /locus_tag="ACHAUN_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461520.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1534..>1901
                     /locus_tag="ACHAUN_002900"
     CDS             1534..>1901
                     /locus_tag="ACHAUN_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461521.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          693 a          271 c          405 g          532 t
ORIGIN      
        1 tttaatggca aagtggaatg tcatattaag tacaacagaa ccctataatt acgtggggat
       61 gattcaagtt cgacaaggca ataaaaacac tgaagttatg gaagctacaa ttgtcgaaaa
      121 tggtcttcca tatgatttat cagaatgtaa ggtatatttt gaatcagttg ttggcggaaa
      181 atatccagtc caattagaaa caagaattgt ggatgctaaa aaagggaaaa tcaaatatat
      241 ttttgataaa tattccatgc agtgtttgca tcgacaaaca gccaatttca ttatatttaa
      301 aggagaagac ttgattggaa caactcaaga cttctcctat tttgtcatca atgctgtttc
      361 aaaaacagaa ggagaaatgg gttcttattg gcaatcaatt gaagatttaa ttgctgatat
      421 gacagatttt attaatgaaa ataaaggcga ttttacggac tggatgaatg aaaggaaaga
      481 agaatttgat cgctggagag aagaacagga gaatagtttc caagattgga gagaaggaca
      541 agaatcagat tatttgtcat ggtttgaatc aatcaaggat attttgaagt ctatcgatcc
      601 aggtggagta atgttagccg aattaatgga tgcacgtgta gatcttcaag gtgtacgcca
      661 tgaatcgatt tctgaacgtt tcctatccga tctaaattat ttgtatcaaa aaatgaaagc
      721 agcacttttc acgattgaat acggtgaaat tgaagtgact gatattcttc aagacgatct
      781 cttttcagat aatcacgaag tcgaaaaaat tgaaacagta gaatttccaa ttgaagaagg
      841 agctttgatt attgcaactg ttgatgatcc aaaacagaat gttttcactc ttgagaaagt
      901 tggggtgatt tgatggttaa agtaaagaga atgatggaga ctgatgaatc tggtgttaaa
      961 cgtcaattta tgccaattac acatgtttct gcagttttag gcttagaaaa aatgatatca
     1021 ggacaatcaa aagttctttc ggttaatggt aaatatggtg cagtcatttt aacgaaagct
     1081 gatttaggat tagagaatgc aattactgaa ctaccttatg caagcgagac aagcgatggt
     1141 attcttactg ctgaaatgtt tcaaaagatc gtaaatggag aaggtggaac atatattttg
     1201 ccgattgcta ctcctgaaca attaggaggt ataaaagttg gggaactctt agaagttact
     1261 gaagaaggtg ttttatctgc aactaagcaa acagacttca atttctctga agaattaaag
     1321 ggaaaattag aatctctaaa aatattaaaa gcaggtgcaa atatatcaat tgcagaagat
     1381 ggaacaatta gttctacagg tggtagtggg actggtggtg ttaatcagtc atatgtagat
     1441 caaaaattcc aagaagctgt aaatcaagca gaaaattaca caaacgaaag aattccaaat
     1501 tttacttttg aaaaaatcgg ggaggtatag aaaatgacag atatcgtaaa aataaaacaa
     1561 agcggtgtac aggtttatcc acaaactcat tggaatgcta tagaaggcaa accaacaacg
     1621 gtgaagggag ataaaggcga tcctggccaa gcagcaacaa ttaccatagg aaccgtttct
     1681 agtggttcaa ccgcttctgt tacgaatgta ggaacatcat ctgcagctag atttaatttt
     1741 gttttaccaa agggagataa aggtgatcct ggaataaatg ccacaactac agctgtagct
     1801 acaactacag caaatgggtt gatgtcgtcc acagataaaa ctaagttgga tggaattgca
     1861 gctggagcac agaaaaatcc aggtaatgct acaactacga c
//
LOCUS       DAYYBG010000198         1895 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000198 DAYYBG010000000
VERSION     DAYYBG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.206"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1693)
                     /locus_tag="ACHAUN_002901"
     CDS             complement(227..1693)
                     /locus_tag="ACHAUN_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8461522.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          596 a          374 c          320 g          605 t
ORIGIN      
        1 aaaaacaagg gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat aaatggtgtt
       61 caatcagctg accttcagca ttagctcaaa aaacggcaaa atatgaggag ctatttttgt
      121 ttggctgttc ttattacttg atgcaggaca gctttttcac aacctctgat tcacggtgtt
      181 ccagaagtag cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
      241 ctgtttctgg ttcagtaatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
      301 tacctaataa gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg
      361 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
      421 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
      481 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
      541 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
      601 tatctttgat accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
      661 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
      721 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
      781 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
      841 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggga
      901 tattatcgct agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
      961 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
     1021 tgacataagg ggatattttt tccgcccctc caactgcaaa tactagcatc gaaacggttg
     1081 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
     1141 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
     1201 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
     1261 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
     1321 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccacca gcaatcagaa
     1381 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
     1441 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
     1501 tatccttgaa ggctgatcct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
     1561 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
     1621 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
     1681 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
     1741 ttcaattttt aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat
     1801 acacatcgtt ttttcatatt ttttccatca aaaaaataag ttattttctt ttatttcatt
     1861 gaccttattg aatttcaagc tattttgaat tttct
//
LOCUS       DAYYBG010000199         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000199 DAYYBG010000000
VERSION     DAYYBG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.207"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHAUN_002902"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHAUN_002902"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8461523.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAYYBG010000200         1819 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000200 DAYYBG010000000
VERSION     DAYYBG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.208"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..233
                     /locus_tag="ACHAUN_002903"
     CDS             <1..233
                     /locus_tag="ACHAUN_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461524.1"
                     /translation="GDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIFMIVIWFLNE
                     RSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(485..1057)
                     /locus_tag="ACHAUN_002904"
     CDS             complement(485..1057)
                     /locus_tag="ACHAUN_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8461525.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1073..1678)
                     /gene="fic"
                     /locus_tag="ACHAUN_002905"
     CDS             complement(1073..1678)
                     /gene="fic"
                     /locus_tag="ACHAUN_002905"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8461526.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          536 a          367 c          271 g          645 t
ORIGIN      
        1 aaggagactt gaatgccatt attactttaa tcgtagttat ggtaaaatca ataggtaata
       61 tcacaatagg aatgctgttt attatattag cagcagtgct gatttttatg atagttattt
      121 ggttcttaaa tgaaagatct aaagacaatc aacaccgtag ggatatggag ataaaatcaa
      181 tagagtcaat agattattta tcgataataa cactaaaaaa gccacgttcc taaacgtgac
      241 ttacgctgaa cgatatgtgt tgtttggtag acgaacaaat cgttcattat aaatatatga
      301 tggtatccta atatagtaag cgtggctagc atacctttaa ttactttacc ttttacagtt
      361 accatacaca ttttttgagc agaaatcaag taaataaaaa aatattatat ttcacaacat
      421 gctaatagac tcatatctag aacgcattaa gtatttacct gtctggttcg ctgagacttc
      481 ttttttaact ttcttcaatt ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg
      541 ttatagcttc aacttcctta tacgatttac ctgaagtaag cagttcatat gcgtgcctct
      601 tcttcggtgt gattgttgct ttcggacgtc cttctctgaa attaggatta ttctttcttg
      661 caaacatttt tccttcttgg gtacgttcca caatcatatc acgttccatt tctgctacac
      721 tcaacaacgt ccgtgtcacc attcgcccca tggacgtatt ttcaatcgtt cctagattca
      781 aaaccttgat cgttacgtta tcatcgagta aaggttcaat gatctctaat gcttctctcg
      841 tgttacgagc aaatcgatca agcttcgtaa caactatttc atctccaggc ttcagaccag
      901 ccaacagctg attgaatttt ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa
      961 tattcttctt ctctacgccg ttctcaagca actgttgaat ttgttctttt gtactttgag
     1021 aagcggttga gacacgtgca tatccatatt tcgtcatttg gtccacctac ttttacaaat
     1081 catctatcgt atactcagaa tacccttcat agaaatagct gacatcgatt cctttcatat
     1141 ataactcgcg atcgtcaatt ttatcagtca gagcattgga aattagatag cgtatttcca
     1201 aatcgtttat gggacttcgc tccattgcag aaagatagtc tgctttatcg attaagttcc
     1261 aatctacaac tttttgcaat tcttccttta aaatgagatc cagccaaatt ctagtacttc
     1321 ggccatttcc ttctcgaaat gggtgagcga tattcatctc cacatatttc ttgataatct
     1381 cttcaaaaga agtttgtggc atctgatcaa tatgctgtaa cgaatgttct aaatacataa
     1441 ctggtgcaaa tcgaaaattt ccttttgcaa tattgaccga tcggattttt ccggcaaaat
     1501 cgtaaatatc cgaaaataga aattcatgaa tttcagataa acctttaaat gtaccaactt
     1561 cgatttcatt tatttttcca caatcgtaca attctttcgc tttttgttta ctgagttttt
     1621 cttcaacctt agccaactca acttgattgg ttaatcccaa tttattctct aaaatcatgc
     1681 gaaatctcct taactttatg acataagttt atgaaacact ctcaacccca ttatatcgcg
     1741 attttgttta tgtgtcaaaa actattaagt ttctgacccc cgatagattt ttcaagtgtc
     1801 tctattttgc acagtctag
//
LOCUS       DAYYBG010000201         1805 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000201 DAYYBG010000000
VERSION     DAYYBG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.209"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..>1805)
                     /gene="uidA"
                     /locus_tag="ACHAUN_002906"
     CDS             complement(12..>1805)
                     /gene="uidA"
                     /locus_tag="ACHAUN_002906"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8461527.1"
                     /translation="MTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESYPISVP
                     SSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNGKFITQ
                     HKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKKENLVN
                     FDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILIAIYDE
                     EAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGIRSVQV
                     TNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYS
                     EEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKTVIKEL
                     IERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPD
                     VCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTEYGADT
                     VAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILRVQGNK
                     KGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          549 a          375 c          223 g          658 t
ORIGIN      
        1 aaaaatccaa attatttttt ataactgaaa ttgggtattg atgaccatcg tttctgtaga
       61 tagtaagcaa tcattttagg ttttctatct ctagtaaaga ttcctttctt atttccttgc
      121 actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat tccacacttg ttcaccaaca
      181 aaattttcac atgtatctat tacttcgtga tttgcttcgt aataagccac ttgatactct
      241 tctgtaaaca ttgttggtac tgtatcatga aatccagcta ctgtatctgc tccatattct
      301 gtaaacatta ttggcgtatc aggacatctt tccatccatc catctagctc ttttttcaac
      361 agatattttg catgtgccag atcccctccc gaaacatacc aaccataata acgatttaaa
      421 cataaaacat cgacaatatc tcctacctta caaacatctg gcgttgattt tagataagta
      481 acgattgtta caggtctttt ctgcggatca agttctttag ttaattcaac taaaggttca
      541 aaatattctt tagcaccttc ggaatccgaa tctggttcat ttgcaataga ccacattaca
      601 acacttgggt ggttcttgtc gcgttctatc aattccttga tcaccgtttt atgagaattt
      661 tctgttcgta tctctttcca cgtattatgt ttttccacat tatcatctaa taaagtggcc
      721 atgaaattta aatgtaaccc tactgcaggt acttcatcaa taactacaat accttctcga
      781 tctgctaatc gcataatttc ttctgagtaa ggataatgtg atgttctaaa agaattagca
      841 cctatccatt tcattaaatt aaaatcttta atatttacag cctcattcaa accacgtcca
      901 ttaacatacg aatcctcatg cttaccaaat cctttaaaat aaaaaggttt attattaatc
      961 aaaaatttgc cattggtaac ctgaacactg cgaataccaa attcttctgt ataggtatcg
     1021 atcactttat tatcatcaat taaactaatt ttcatactat ataaataagc gtctagaggt
     1081 tcccaaaaaa ttggattctt aagctgaatc attcctttct ttccttcagc ttcagctaca
     1141 agttgagcct cttcgtcata tatcgctatt aagatttttc cggtccattc tgtatcgata
     1201 ttgaattcta cacttgcatc caaatcagtt attcttgtat taatagtaat atcacttata
     1261 tagtctttcg gagttgtata taacttaaca ggtctatgaa tacctgcata attgaagaaa
     1321 tcaaaattaa ctaagttttc cttttttata tccccatttt cctgtactgt ttccttcata
     1381 atccccacag gtaaagtact atcgtcaata atattgttta cagcaactgt aattcgatta
     1441 ttcccgtctt ttacaaaccg attaatttca gactcaaatg gtgtaaaccc acctttgtgt
     1501 tgagtaataa actttccatt caaaaaaact tttgcttcat gtgttgcaga tccaaaacga
     1561 agaactaatc tttcttctaa taatctttta ggaagagtca cttctttttc ataccacacc
     1621 catccaacat gatttctaat gttctctgtt tcaaataaat cattataaga agagggaact
     1681 gaaatcggat aactctcttt ttcatttatt ggttctaaag caagttcttc ctgtaagtca
     1741 tcatttgtat ctaatttaaa tctccagaga ccgcttaagt caattacttg cctcgtctct
     1801 gtcat
//
LOCUS       DAYYBG010000202         1802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000202 DAYYBG010000000
VERSION     DAYYBG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.210"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..898
                     /gene="erm(B)"
                     /locus_tag="ACHAUN_002907"
     CDS             161..898
                     /gene="erm(B)"
                     /locus_tag="ACHAUN_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8461528.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1068..1328
                     /locus_tag="ACHAUN_002908"
                     /pseudo
     CDS             1068..1328
                     /locus_tag="ACHAUN_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1462..1701
                     /locus_tag="ACHAUN_002909"
     CDS             1462..1701
                     /locus_tag="ACHAUN_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8461529.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          707 a          273 c          311 g          511 t
ORIGIN      
        1 aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa ttcctaccag
       61 tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc gtttaacttc
      121 tgtatttgtt tatagatgta atcacttcag gagagattac atgaacaaaa atataaaata
      181 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      241 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      301 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      361 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      421 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      481 acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc tgattgttga
      541 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      601 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      661 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      721 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      781 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccattac
      841 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      901 ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat ggagataaat
      961 tattagatat actactgaca gcttccaaga agctaaagag gtccctagcg cctacgggga
     1021 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
     1081 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
     1141 ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg
     1201 taaaagaatt actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa
     1261 agccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atatctttct
     1321 caaaataaac ataaaagccc tttaaagagg gcttttatat attaatcaca aatcacttat
     1381 cacaaatcac ttatcacaaa tcacaagtga tttgtgattg ttgatgataa aataagaata
     1441 agaagaaata gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa agaaaaacga
     1501 aatgatacac caatcagtgc aaaaaaagat ataatgggag ataagacggt tcgtgttcgt
     1561 gctgacttgc accatatcat aaaaatcgaa acagcaaaga atggcggaaa cgtaaaagaa
     1621 gttatggaaa taagacttag aagcaaactt aagagtgtgt tgatagtgca ttatcttaaa
     1681 attttgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
     1741 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
     1801 ca
//
LOCUS       DAYYBG010000203         1742 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000203 DAYYBG010000000
VERSION     DAYYBG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.211"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACHAUN_002910"
     CDS             5..403
                     /locus_tag="ACHAUN_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8461530.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACHAUN_002911"
     CDS             387..1082
                     /locus_tag="ACHAUN_002911"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM8461531.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          648 a          254 c          336 g          504 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 gg
//
LOCUS       DAYYBG010000204         1701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000204 DAYYBG010000000
VERSION     DAYYBG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.213"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..845
                     /locus_tag="ACHAUN_002912"
     CDS             174..845
                     /locus_tag="ACHAUN_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8461532.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(1005..1196)
                     /locus_tag="ACHAUN_002913"
                     /pseudo
     CDS             complement(1005..1196)
                     /locus_tag="ACHAUN_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          611 a          259 c          226 g          605 t
ORIGIN      
        1 ttcagaaaaa taaaatactc cttctttttg gactgatttt atcgaaactt attttttctt
       61 tttttcatgt attcttttat ttttataact agtgtattac ttcaaaattt gatattatat
      121 ggcatgaaca gaattaaaat atcagttaaa ataaaagtta aggagcattg tgtatgacaa
      181 ttaaaactag tctccaaaat caagcttacc aatctattcg ccgacaaatt atctatgctg
      241 acttagaacc tggaaaaaaa gtatcagaaa aaaaattaga agaaacactt tctattggtc
      301 gtacaccaat acgtgaagca ctgattcaac tccgacaaca aggtcttgtt gatacaattc
      361 ctcaaagcgg aaattatatc tcattaattg atttagattc ggccaaaaat gctcgatttg
      421 tcagagaaca tttggaacga caaattatgt tagagtgttg tgccaaaatg aataaccaaa
      481 aaaaacggat tttagaaact ataatttcag aacaagaaaa agctgttagt caaaaaaatg
      541 ctcgtagttt ttttcataat gataatttat tccataaagc ttgttttgaa attgcagagc
      601 gagaagaagt atggcaatgg ttagaaaata acaatacaca cttagaacga tttcgatggc
      661 tacgagtaaa aacggaagga ttaaagtggg aaaaaattat ggaacaacat tatcaactat
      721 ttgaggcatt aacaaaacaa gatccagaag aagctaactt tctaacatcg ttgcatcttc
      781 atttaatgct taatgagcaa aatattgttt tagctactca tccaaaatat ttcaaaaata
      841 tataactttt aactcttttt aaattaaatg gttgttctga tcagaaaaat atcgcttgtt
      901 ttcttattat gtaatatatt tcataaacta ttctgcataa caactattgt ttgtactttt
      961 tacattggat aactaattat gactgctgat aacttttgat attaaactag tatctccaat
     1021 acaaataaaa aatagatatc ttgttcatca aaattagttc gtggatactt accttcattt
     1081 acatcattca gcggtttccc attttctttt attctattat ttcattttct aatagacttg
     1141 gcttcatttc gcaatacttt ttttcaatag gcatcaaatt ggtgttgttt agatatttag
     1201 ttgtagtttt gtgacatgat aagtatgctc ctttttctct tttccactaa tacaacgatc
     1261 aaagggtgct tttttcaaaa aactagaaaa atcaaaataa gattcgtatt ttgattctat
     1321 atatcctgtt tcaactacat cttatatttc acaatagaat aaatagcctt ttgcatagct
     1381 gcttaactta taagaaattg caatttactt cttcaaagtg aaaacgaata tgagtaaaat
     1441 aaaattattc ttcttacaga taattaaaaa tagcgcgtaa caaaaatcgg cattcaatca
     1501 cataattagg attgatgccg attacttata ttctacgtat agttttagta ttcttatttt
     1561 ttatcaccag taaccaattg agaacagccc taaagcagaa tagtctttta tctcaaaacg
     1621 cttaatttca ggcttaggaa tatgaataat cttttgagta tattctttct cttttaatct
     1681 tactgaagaa ataccactgc c
//
LOCUS       DAYYBG010000205         1631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000205 DAYYBG010000000
VERSION     DAYYBG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.214"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHAUN_002914"
     CDS             131..505
                     /locus_tag="ACHAUN_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8461533.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHAUN_002915"
     CDS             complement(619..1248)
                     /locus_tag="ACHAUN_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8461534.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHAUN_002916"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHAUN_002916"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          572 a          238 c          279 g          542 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt a
//
LOCUS       DAYYBG010000206         1581 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000206 DAYYBG010000000
VERSION     DAYYBG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.215"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(846..1376)
                     /locus_tag="ACHAUN_002917"
     CDS             complement(846..1376)
                     /locus_tag="ACHAUN_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFM8461535.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1373..>1581)
                     /locus_tag="ACHAUN_002918"
     CDS             complement(1373..>1581)
                     /locus_tag="ACHAUN_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8461536.1"
                     /translation="NIKKPKIWSSIVYGGKIAFYFSAIIEFLQLFLRLGTFQISDFVY
                     NTLGGILGVILYKLILITKRDGER"
BASE COUNT          487 a          316 c          181 g          597 t
ORIGIN      
        1 taacattgat tctcctatga aaatatcttt tacctttctc ctgtgaaaag gaaatttcct
       61 tttttatagt gttattgata taatcagaat attcatcaaa ctctgtatct ggagttaaaa
      121 atctacagta acatctataa gctttgctag taatcttctt gggttctctc tccattgtca
      181 agcaacatca aaacacctac ttactattcg attcttttct gccaaaaacg agtcagcatc
      241 actcacaact tatgtgttgc tgactccgac tagtcctttg ttcaatatca acttattaaa
      301 cagatataat atttttcttt atttcgtata ctccaagact tccatctttc tttcatctta
      361 tctctgaaaa aaacacagca aagtgccctt aataaatata cattagaatt tgcaaatttt
      421 ttcatcttaa gatatatcct attcatattc tttacaatcc taatgggtga ttacaaatct
      481 atttagttca agttctacat tataaacacc tttcttgata gtatcatctg ctgcacaagc
      541 gatgtcgatc cacttattag gggatttacg tgctcaaaag tggctattcc ttttgggaag
      601 tccatccata ataatggtcc atggattttt ttagggtctt gtcgttcaaa tttctcaaaa
      661 actttattat acttccatag ttttataatt atttggtttc actttggctc gttcaaacac
      721 caacttgtat ttcttttcaa cgttcgaact ggagcccccc ctataatctc attattattt
      781 tcatgacttt ttactaccgt ggaattacaa gcaacaacaa tattatctgc taaatgaaca
      841 cctttctata tcacgctttt tgctccaatc caacaatgtt tacctataac tattttatta
      901 tttttattca aaatcctatg agtattcaat tcaaaaatcg tatgtgaatc ggtatccata
      961 aataatgtat cccacgatac tgtacagcca tctttgaatt gaatttcctt aaagcaaatt
     1021 atactcattt ttcctgtcga attaaacttt gacccaatat ctaaaactgc atttttcctt
     1081 atgtcaatat gagccccttg tgcgataaac gctggccctt gtattcttag tattccttca
     1141 atacttataa tcgtttcttc atttcccgca gtatttaata ctggaaagcc caaataaact
     1201 atttttccag attgtaatac tatctcaatt ttccctctcg atttgcgtgt aatccgtact
     1261 ttacgatgca ccaaaatagg taatctccac gcatctgaaa tatgaagaaa tttataattg
     1321 aagtatatcg ttttaggtaa atttaatata ttctttatta tcaatccgag tctcatcttt
     1381 caccatctct ttttgtaatc aaaattagtt tataaaggat tacacccaat ataccgccca
     1441 acgtattata tacaaaatcc gaaatttgaa acgttcctag cctcaaaaaa agttgtaaaa
     1501 attcaattat tgctgaaaaa taaaatgcaa tttttcctcc atatactatt gaggaccata
     1561 ttttgggttt ctttatattt t
//
LOCUS       DAYYBG010000207         1536 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000207 DAYYBG010000000
VERSION     DAYYBG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.216"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACHAUN_002919"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACHAUN_002919"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAYYBG010000208         1522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000208 DAYYBG010000000
VERSION     DAYYBG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.217"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..1478
                     /locus_tag="ACHAUN_002920"
     CDS             12..1478
                     /locus_tag="ACHAUN_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8461537.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          323 g          493 t
ORIGIN      
        1 gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg aagggattgt
       61 caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg cgtgcattac
      121 ttatctatta tatgtacgat acagttgcca acgggggact tggattacca cgagcaacag
      181 cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc gtcggcggat
      241 gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga gttttcatta
      301 tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt gtttcgatgg
      361 cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg gtcggacatc
      421 tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac atgggtgtca
      481 acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa tataactatc
      541 atttagggtt ctctttagct gctattggga tgttctttgg attattacaa tacatcatcc
      601 aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg acaactgaag
      661 aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct gcagtattcg
      721 gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca atcagtgtat
      781 tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa gatgtaactg
      841 ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca atcattttct
      901 ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa cgtacacaat
      961 ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat ccaattttca
     1021 tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa cgtcaaccat
     1081 ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac ttgctattga
     1141 tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg ttgttgggaa
     1201 gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg tctgtgacaa
     1261 caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc ttagctgatg
     1321 cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga acagaatctg
     1381 cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta tttactgtga
     1441 aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata aaaacaggaa
     1501 ttgcttattt gcagcagttc ct
//
LOCUS       DAYYBG010000209         1470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000209 DAYYBG010000000
VERSION     DAYYBG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.218"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..620
                     /locus_tag="ACHAUN_002921"
     CDS             <1..620
                     /locus_tag="ACHAUN_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8461538.1"
                     /translation="VNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICHPIIYK
                     SIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKLMESYI
                     GNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENASFFDT
                     DLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            743..1381
                     /locus_tag="ACHAUN_002922"
     CDS             743..1381
                     /locus_tag="ACHAUN_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8461539.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          499 a          225 c          229 g          517 t
ORIGIN      
        1 aagttaatga agcaagcttg aataaaggat ccttttttca tatctatgaa aacgaagatt
       61 ttgaactgta taatatcctt tcacaaaaag aggtcatcga aaaccaaata atctgccatc
      121 caattattta caaatctatt ttcaagtatg cattttttgc ttccaatgat tttcaaagaa
      181 tggagattac cgactgtata ttcgaaaact gtgatttttc aggctgtata ttgacaaaaa
      241 gcatgttgca tcgcgtgaaa tttgtcaatt gtaaattaac aggtactaaa ttgatggaaa
      301 gttatattgg gaacacgtta ttcgagaatt gtaaaatgga ctatgtaaat ttatctggtt
      361 caaacataaa agaaagtacc tttgagcact ctgtcttatc aagcgcagat tttgtagact
      421 gttccctcac taaaataatg ttttctacta atgatttaga aaatgctagc ttttttgata
      481 ctgatttaaa aaatatcgat ttaagtagca actcctttga aatgattgaa gtacgctcgg
      541 aacatctaaa aaattgtacg cttaatcagt tgcaagcact tggttttgct agaaaatttt
      601 tacaaataaa agtagtataa tcaaaatgaa tatataagat aaatttttag caatacaaat
      661 atccataaaa aaacattgcc tattttaact cttcatgtat actgactcta tcttcaaaaa
      721 ctgaatagta ggagttatta tcatgaataa cattttagga aaaaaaatag aacttaaaaa
      781 agggatattg ggcactattg tcgttgtact cggtttgtta gggttgctat ttgcgccatc
      841 cattattagc attttttctt taactatcac tggatttata gcatttacac ttgtttttgg
      901 taaaaaagat acgaaaaaaa tgttctcgaa acctgtcgct cctgtcaaaa atatagccaa
      961 atattttttt atcaatgtag taatcagtgc cgcagtctcg gtatttttgc aacaaatttt
     1021 gaaatggggt ttaacaggca atccaataaa tgaggctttc agcccgttat tatttattat
     1081 tttgcctatc atgatacttg gagaagaatt gttctcagtg tattttctcg ctattttttc
     1141 tagtaagttc agcattcctg tcgctagttt cttgtcagca atcatctttg gatttatcca
     1201 ttacagtact tatgataatg gaaatatttt acacactgta gcacacatcc ttttgataca
     1261 aggggtggca agattgttat tcaaccaagc tgccattaaa agcaactcga tcattacaag
     1321 ttgggcagtt catgtgatat ttgatttcac agctatattg ctagtcgttt tattctccta
     1381 aatttttata gaaataatac atgctctatt gctaagaaag catactttat ttcctttaaa
     1441 caaaaagaca gatgtatcaa tggattaaaa
//
LOCUS       DAYYBG010000210         1432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000210 DAYYBG010000000
VERSION     DAYYBG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.219"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..460
                     /locus_tag="ACHAUN_002923"
     CDS             197..460
                     /locus_tag="ACHAUN_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8461540.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            460..732
                     /locus_tag="ACHAUN_002924"
     CDS             460..732
                     /locus_tag="ACHAUN_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8461541.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            809..1138
                     /locus_tag="ACHAUN_002925"
     CDS             809..1138
                     /locus_tag="ACHAUN_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8461542.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1128..1415
                     /locus_tag="ACHAUN_002926"
     CDS             1128..1415
                     /locus_tag="ACHAUN_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8461543.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          535 a          175 c          283 g          439 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggtataaag gaaacggata acactttaca atgctgtttt ggtactccaa
      121 tttaaacaaa acaatcgaat ttacgtactt aattaagtac gttagaatgg agatataaat
      181 tagaggggga gtagatatgg caaaaacagt cttaaatcct agctcagcac ggaaaaattt
      241 ttataagttg ttgaaagatg taaatgagaa tcatacggaa attgaaatca ttagtgagcg
      301 cagtgaaaat aatgcggtgc ttattggttt ggatgattgg aaagcaatca aagaaacgct
      361 attacttgag caaataggca ctttgggtgt tgttcgtaag cgagaaaaag atgatagtgg
      421 atttactaat gttgatgaaa ttgattggga taacttataa tgaataacta ttctgtgatg
      481 attaaaaact cggctaaagc tgatttaaag aaaatcaaac agtctaattt gaaacctcaa
      541 tttgagaagg ttattcagac tttaaaggaa gatccctatc tccctaccca atcatttgag
      601 aagttaaaac ctacacatga aggtaggtat tctagaagat tgaatagaca acaccgggtt
      661 gtttataagg tagatgaaga tgaacacgtg gttgaaattt attcagcaag gacgcattac
      721 gagtcacagt aattacttgt aaatacatta tctcgtggta taatgtattt actaagtaat
      781 tacatactgt tagaaatgag ggatttaaat gattacaaaa acaagaaaac aaggcaattc
      841 aatcatgttg acagtaccta aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa
      901 gttggtagag aatggtattc tttatgaatt tgttgaacca caaaaagaat tttatgattt
      961 tagcgaagat attttgtctg atataattgc agaaggctac gataaagatg agattcttgt
     1021 agaatttaag aatagaaaaa acaaaatgca ttcttccttt agagatattg ctgaagatac
     1081 acttactaat tctaaagtaa tgactaaaga ggagttggca acagaaattg gcttatgaaa
     1141 tcgttccttc aaaacatgtc attaagtatt taaaaaagct aaaagagaag ccattgaaag
     1201 aaaaattttt gaatttaatt tatgatgaaa ttgcgattga tccatataag ggtgaacaaa
     1261 aaactgggga tttatcaggt atttggtcta gtgggttcaa atatgcgggc acaacatata
     1321 gagtagctta cgagattata gaaaacaagg ttattccagt attgttatgt ggaacgcatg
     1381 aaaactttta tgaacagttg aaaaaattaa tctagtgatt tgttgtcgtt ct
//
LOCUS       DAYYBG010000211         1417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000211 DAYYBG010000000
VERSION     DAYYBG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.220"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACHAUN_002927"
     CDS             complement(150..1337)
                     /locus_tag="ACHAUN_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8461544.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAYYBG010000212         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000212 DAYYBG010000000
VERSION     DAYYBG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.221"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACHAUN_002928"
     CDS             82..>1364
                     /locus_tag="ACHAUN_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8461545.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAYYBG010000213         1311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000213 DAYYBG010000000
VERSION     DAYYBG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.222"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(428..784)
                     /locus_tag="ACHAUN_002929"
     CDS             complement(428..784)
                     /locus_tag="ACHAUN_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8461546.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(798..1208)
                     /locus_tag="ACHAUN_002930"
     CDS             complement(798..1208)
                     /locus_tag="ACHAUN_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8461547.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          416 a          241 c          181 g          473 t
ORIGIN      
        1 gatttcgttt ttcgaaatct tacagcaact catttatcat aacttatcat ttgttatttg
       61 tcaagaactt ttttgaagtt tttttcgata gaatcaagct tcacaagtgg ttctttttta
      121 acaactcagt tatcttatca tgtcatttct aacatgtcaa caactatttt atatgttttt
      181 taaaatacta ttttattgct ttgctactta aaacaacaga aatgaatata tcatgtaaat
      241 ttacacatgt caatatattt ttaactctat atccccacct agatttgaat tttgaccaca
      301 taaattgagc atgtctctat agtagaaaaa taaagtcaaa aaaagaacac ataacacgct
      361 tgatgctaag tgttcgactg ttacaactgt atgaaatgct cagtgacttg attaaaacag
      421 ttaaagtcta tttatttaag ataagctgtt ccatcaactg ctcattgtat ccaataaata
      481 gaacttcatc gataataatc aaaggacgtc tgaggagatt ggtatgttgt gtgcataaaa
      541 aaatcagttg tttttttcct acacaggaaa ggtcaatcgc aatgttgtga aaagcctcag
      601 accaagtcgc aaatagctgt tctgcttgca cttcttcaaa actgcataat tcttctgctt
      661 cttctgtact tagatcgcgt tcttctaagt caacaaattg ataaggcaat tgatggtgtt
      721 ctaaccattg tttcgccttc agacttgtta cacagtgcgc tgatccatag actttcactt
      781 ccacgaaggc tccctcccta tgaatttaag atgttattgt tttgtaaata gatcagattt
      841 ctggcaacac atcgaatttc acgagggaca aatgaccgga tctcatcttc attaaatccg
      901 attagtattc ttttttcatc aatcaaaata ggtcgtctca ataatgaagg atattctttg
      961 aataagacga tcatttgctt taaagttaat tcgttaaaat ctatgcccag cttttcgtaa
     1021 attttcgacc gagtggaaat aatatcttga actcctcctt cagtcgctgc caagatcttt
     1081 ttgatctcac tttctgttaa cggttcggca aaaatattct tctcttcaaa ttcaagttga
     1141 ttcgcttgca accatgctct tgcttttcta caggaagtac agctaggcga agtatatact
     1201 tttatcattt tctcactcct ttttaattat aatccaattc tacagtaatt tcttttaaaa
     1261 taccaattat tagctaataa aatataaaaa aaatcaaaat aactgattat t
//
LOCUS       DAYYBG010000214         1302 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000214 DAYYBG010000000
VERSION     DAYYBG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.223"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..>1302
                     /locus_tag="ACHAUN_002931"
     CDS             52..>1302
                     /locus_tag="ACHAUN_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8461548.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DAYYBG010000215         1298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000215 DAYYBG010000000
VERSION     DAYYBG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.224"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACHAUN_002932"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACHAUN_002932"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8461549.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          445 a          173 c          220 g          460 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaa
//
LOCUS       DAYYBG010000216         1294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000216 DAYYBG010000000
VERSION     DAYYBG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.225"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACHAUN_002933"
     CDS             <1..1244
                     /locus_tag="ACHAUN_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8461550.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAYYBG010000217         1292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000217 DAYYBG010000000
VERSION     DAYYBG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.226"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..89
                     /locus_tag="ACHAUN_002934"
     rRNA            <1..89
                     /locus_tag="ACHAUN_002934"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            184..299
                     /gene="rrf"
                     /locus_tag="ACHAUN_002935"
     rRNA            184..299
                     /gene="rrf"
                     /locus_tag="ACHAUN_002935"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            313..385
                     /locus_tag="ACHAUN_002936"
     tRNA            313..385
                     /locus_tag="ACHAUN_002936"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:346..348,aa:Val,seq:tac)
     gene            389..461
                     /locus_tag="ACHAUN_002937"
     tRNA            389..461
                     /locus_tag="ACHAUN_002937"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:422..424,aa:Lys,seq:ttt)
     gene            469..550
                     /locus_tag="ACHAUN_002938"
     tRNA            469..550
                     /locus_tag="ACHAUN_002938"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:503..505,aa:Leu,seq:tag)
     gene            568..640
                     /locus_tag="ACHAUN_002939"
     tRNA            568..640
                     /locus_tag="ACHAUN_002939"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            652..723
                     /locus_tag="ACHAUN_002940"
     tRNA            652..723
                     /locus_tag="ACHAUN_002940"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:684..686,aa:Gly,seq:gcc)
     gene            740..825
                     /locus_tag="ACHAUN_002941"
     tRNA            740..825
                     /locus_tag="ACHAUN_002941"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Leu,seq:taa)
     gene            836..909
                     /locus_tag="ACHAUN_002942"
     tRNA            836..909
                     /locus_tag="ACHAUN_002942"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:acg)
     gene            919..992
                     /locus_tag="ACHAUN_002943"
     tRNA            919..992
                     /locus_tag="ACHAUN_002943"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:953..955,aa:Pro,seq:tgg)
     gene            1012..1084
                     /locus_tag="ACHAUN_002944"
     tRNA            1012..1084
                     /locus_tag="ACHAUN_002944"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1045..1047,aa:Ala,seq:tgc)
     gene            1112..1185
                     /locus_tag="ACHAUN_002945"
     tRNA            1112..1185
                     /locus_tag="ACHAUN_002945"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1146..1148,aa:Met,seq:cat)
     gene            1199..1272
                     /locus_tag="ACHAUN_002946"
     tRNA            1199..1272
                     /locus_tag="ACHAUN_002946"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1233..1235,aa:Ile,seq:cat)
BASE COUNT          314 a          288 c          362 g          328 t
ORIGIN      
        1 cctgagagat gatcaggtag ataggtcagg agtggaagta cagtgatgta tggagcggac
       61 tgatactaat cgatcgagga cttaaccaat aggttaaaag aaaagaactc ggaaaaaaac
      121 ttatgtgact tcaaatccag ttttgagtga acaaaattta ctcaataaaa gataacacca
      181 cagtgtggtg gcgatagcga gaaggataca cctgtaacca tgccgaacac agaagttaag
      241 cttcttagcg ccgattgtag tgaggggttg ccccttgtga gagtaggacg tcgccacgct
      301 taagttatcc atggaggttt agctcagctg ggagagcatc tgccttacaa gcagagggtc
      361 agcggttcga tcccgttaac ctccatatga gccgttagct cagttggtag agcatctgac
      421 ttttaatcag agggtcacag gttcgagccc tgtacggctc atatttttgc gggtgtggcg
      481 gaattggcag acgcactaga tttaggatct agcgccttac ggcgtggggg ttcaagtccc
      541 ttcacccgca taatttaagt tttacatgcc ggcttagctc agttggtaga gcatctgatt
      601 tgtaatcaga gggtcgaggg ttcaagtcct ttagccggca tctttaaaca cgcggaaata
      661 gctcagtggt agagcaccac cttgccaagg tgggggtcgc gggttcgaac cccgttttcc
      721 gctttgccaa agcaatcgcg ccggggtggc ggaactggca gacgcacagg acttaaaatc
      781 ctgcggtgag tgatcaccgt accggttcga ttccggtcct cggcatttca aatatgcgcc
      841 catagctcaa ctggatagag tgtttgacta cggatcaaaa ggttaggggt tcgactcctc
      901 ttgggcgcgt taattaaacg ggaagtagct cagcttggta gagcacttgg tttgggacca
      961 aggggtcgca ggttcgaatc ctgtcttccc gataattata aatttaaaca cggggcctta
     1021 gctcagctgg gagagcgcct gctttgcacg caggaggtca gcggttcgat cccgctaggc
     1081 tccatttcat ttaaataatg aatatctttt tcgcggtgta gctcagctgg ctagagcgtc
     1141 cggttcatac ccgggaggtc gggggttcga tcccctccgc cgcgattttt aagagcctgg
     1201 acctttagct cagttggtta gagcagacgg ctcataaccg tccggtcgta ggttcgagtc
     1261 ctacaaggtc cattgtggcg ttgtaagttt ca
//
LOCUS       DAYYBG010000218         1235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000218 DAYYBG010000000
VERSION     DAYYBG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.228"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            281..973
                     /locus_tag="ACHAUN_002947"
     CDS             281..973
                     /locus_tag="ACHAUN_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8461551.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          527 a          167 c          200 g          341 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactaagt agttcagttt tggagtacaa aatgacaaaa tgtacttcaa aatggactac
     1081 ttttttcatt ttaacttaca taccaaatta catacaaaat gatttataaa atgcaattga
     1141 aaatgataat tgaatgaaaa tttgtgattt tgtggtatta tgataatata acttatagaa
     1201 aaaattgctt attcaactaa cgggcaggat aatga
//
LOCUS       DAYYBG010000219         1216 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000219 DAYYBG010000000
VERSION     DAYYBG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.229"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..1157)
                     /locus_tag="ACHAUN_002948"
     CDS             complement(3..1157)
                     /locus_tag="ACHAUN_002948"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8461552.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          342 a          260 c          223 g          391 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagctgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     1201 gtatcataaa aagatt
//
LOCUS       DAYYBG010000220         1207 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000220 DAYYBG010000000
VERSION     DAYYBG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.230"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            203..517
                     /locus_tag="ACHAUN_002949"
                     /pseudo
     CDS             203..517
                     /locus_tag="ACHAUN_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(822..>1207)
                     /locus_tag="ACHAUN_002950"
     CDS             complement(822..>1207)
                     /locus_tag="ACHAUN_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFM8461553.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          439 a          167 c          214 g          387 t
ORIGIN      
        1 aaaccaaatt taaactttat attataataa tgaagcttaa tcttctttat agagaattgg
       61 gctattttta atggcaaaga ggggaagaag atgtttcaaa acaaaggaca acgctatgtt
      121 gtaaaaggag taaaggaatc ttactggaat caaaaatctt tattgagaaa attatctttg
      181 aatttaagca caaagaaatg aaggtacact agatagcaat gaagatatct ttgtcattaa
      241 acgtcgctat tttacacaag taagtgatga tttgattcat gtcttttcca atgagtggtt
      301 tgtttctgag aaaaaattac atgcttctaa tctgcaatat atgccaggtg aagatccaat
      361 tcctaatatg aaagcaatta tcaacagtaa ggattacgaa ggatataaag ccaaacatcc
      421 agaagcgaaa ccatttaaat acccacaaga aatgaagcga gcatggcgta aaatgttaga
      481 tgatgagctg attccccttg aaaatgaatt gaggtaaacg agaagaattc aataaactgg
      541 atgataaaaa tataatatga ctataaattg gttacagctt caacctataa gttgtaataa
      601 attagcctaa aagtaattta ttaatgatga ggtgaatttg attggtacaa caagatatat
      661 cacttagaga gggagaagca gcactacaaa ctacttttca ataagtagga attatagaaa
      721 ttttactcaa aaaatcttca gtaaacaatt tggctaaata agattcagtt gttatacgag
      781 aaggttacgg ttcttcttaa ctaagcttag tatgttttta ttcaaggcat gattttctct
      841 atgtgatcat tcagtttctg taagagttct aataattctt ttttggagta cttgtaattg
      901 ctttctaccc accgattgag aacggataga caaccttgga cgtggtacgt agtaaagaat
      961 ctcatttctt ctgtgataat cgttttcttg tcttcatata gccatatgtt cagatagttt
     1021 tcttcaataa cttgtttaac tttcttctga aacgaaatat caccatggtt gaggataaat
     1081 aagcgatata aaaaagtatt tgcgtaaaca tagtcaatca agcgagtgta tagttcctca
     1141 tagctatggg ctggatcata agagagaatt tcatttagat cccgaaaaaa gttgctttct
     1201 aattgat
//
LOCUS       DAYYBG010000221         1193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000221 DAYYBG010000000
VERSION     DAYYBG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.231"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHAUN_002951"
     CDS             <1..1076
                     /locus_tag="ACHAUN_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8461554.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAYYBG010000222         1179 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000222 DAYYBG010000000
VERSION     DAYYBG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.232"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..1001
                     /locus_tag="ACHAUN_002952"
     CDS             3..1001
                     /locus_tag="ACHAUN_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8461555.1"
                     /translation="MSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLAIPNKIILHE
                     TGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQ
                     IELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVW
                     GNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGT
                     NHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQA
                     YNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          394 a          206 c          235 g          344 t
ORIGIN      
        1 cgatgagcct gttaacggct ctttttttat tacccattaa tacgtttgct tatactatta
       61 atgacgagta taatttagcg ccgaatcaag gagactccag attagcaatt cctaacaaaa
      121 ttattttgca cgaaactgga atagatgcac cagcaagaaa cgtagccgcc aacatgaaaa
      181 ataattataa cggaagcaat tcttatacta cagatgttat tggtgacggt gggattgttt
      241 accgtgtggg tgagcaagga tatgtttcgt ggggagctgg taacgctaat ccttatgcgc
      301 ctgtacagat tgaattacag cgcacatatg ataaagcatt gtttgaaaaa aactatcgag
      361 cttatattga atatacaaga gatagtgcaa aaaaatatgg aattccattg actcttgatc
      421 aaggaacttc tttatttaca aaaggaatca tttctcattt gtgggtgaca aattatgttt
      481 gggggaacca cacagatcca tatggttact tatcgcaaat gggagttagc aaagaaaagc
      541 ttgcttatga tttagctcat ggatttaccg atgaaaatcc aacaacttct gaaaacaagc
      601 ctgtcattga tccaacacga gctggtgcag ctaatcctac gctgacagat ggaacaaatc
      661 acgcccacat tgatcagttt ggggaaatcg aaaacgcaaa cttgcatgtg gctggatggc
      721 acattgctaa ctataaatac gagtatattt tcattatgga ctacaatact gggaaagaat
      781 tagctcgagt aagagctgat ggaatttata gaccagatgt aaaccaagct tataatactt
      841 ctggaaatgt tggttatcat gtatctttca atatgcgtaa ttttcctagt aagaaagtct
      901 atgtaatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
      961 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     1021 agcagtacat aactatattg acaactataa aaattattcg ataaaatagt gatgttatcg
     1081 catatcttca ctatcaccca taaatagtca cactccaagc tatgcgataa caggtttgtt
     1141 gccacacatt ctactggttg attgtttatg gctttatgt
//
LOCUS       DAYYBG010000223         1158 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000223 DAYYBG010000000
VERSION     DAYYBG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.233"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..276
                     /locus_tag="ACHAUN_002953"
     CDS             16..276
                     /locus_tag="ACHAUN_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461556.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(638..877)
                     /locus_tag="ACHAUN_002954"
     CDS             complement(638..877)
                     /locus_tag="ACHAUN_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461557.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          408 a          163 c          216 g          371 t
ORIGIN      
        1 aaggatggat gaaatatgaa taacgaaact atcattttag gaaaaaaata tatttgccaa
       61 cctattggct tgaaacaaaa agtggttgga gaagtaatca gtaaattaga aaattgtgtt
      121 gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga
      181 aaagtagtgg caaaatatac tcacgtttat gagttggaaa caacagaata tctatataac
      241 tatagcaaca aatatgaaca tactttagtt tcataaagat ttaaataaag tgccttcaaa
      301 agcgagaaga ccaagctttt gaaggcactt tatttagttg gcagtgattg ttcatgattt
      361 ttgatttatt caaaaggttc gcctagagaa tctgtctctg taacgaggcc tggtggagaa
      421 actcgataaa aacctatact gccagaactg tcctattttc ggatagacaa actagtagtt
      481 ttaaaaagat catccgatcc aatcatttca aaaaatgcaa aactgatatc aggatcattt
      541 agctcaggct tgttatcgta aagcatttgt aaaggttgca tttagttagg agtaaacgcg
      601 aaactatcat cattatttgc tgaagaagat gaagaagcta attcagagtt tgtttgaaat
      661 aaaggagacg tgctgacagt ttctttaccc tgttttttgc agaggtagaa acttttatat
      721 cggaagtttc taccggagaa tttttctcat ttgtagaaat tgttgaatcg atactattcg
      781 aaatttgcgt atctgaaggt atgttctttg atgcagaagt cccacaagca gtaagtaaca
      841 ataaatttga taaactgata aacagaattc ttttcattat gtacacactc cttatagtat
      901 cgtttagcat acacctatgc tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt
      961 tatagtatct attagtctta gtaatacata tatggagagg gtgatatccc agttcaaacc
     1021 agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt tttatgaaaa
     1081 aatttataag tgaaaacgta ggtatgatat tatctagagg taattttttt gggtatttaa
     1141 atagttgcta ggaggata
//
LOCUS       DAYYBG010000224         1137 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000224 DAYYBG010000000
VERSION     DAYYBG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.234"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACHAUN_002955"
     CDS             <1..355
                     /locus_tag="ACHAUN_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461558.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACHAUN_002956"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACHAUN_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ACHAUN_002957"
     CDS             985..>1137
                     /locus_tag="ACHAUN_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8461559.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAYYBG010000225         1073 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000225 DAYYBG010000000
VERSION     DAYYBG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.235"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACHAUN_002958"
     rRNA            <1..>1073
                     /locus_tag="ACHAUN_002958"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAYYBG010000226         1061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000226 DAYYBG010000000
VERSION     DAYYBG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.236"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1061)
                     /locus_tag="ACHAUN_002959"
     CDS             complement(<1..>1061)
                     /locus_tag="ACHAUN_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8461560.1"
                     /translation="SSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFS"
BASE COUNT          355 a          193 c          125 g          388 t
ORIGIN      
        1 tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc tccctctttc
       61 aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc agaaagatgt
      121 agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt ttcatacaaa
      181 tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg aagcttagta
      241 ctagcgcctt caatagtaaa gagtttaact aattcattta agcagaactc acagctatcc
      301 gagtccatat acttatttaa tttttctaag gtttgattcc cattaattag attttctaaa
      361 tttaaacaat cgattccttg aatgtaaaat tcagtggttg aaagaacaca aattgtattc
      421 ttaaaaatca tagtatcttt ttcctttgct attttcttta atgcgttaaa atcaaaaatc
      481 aaaatctcca cttgattatc gagataccgt tttaaaatat ccttaacttt ttcagaaatc
      541 ccctcaccag agagacaaga tactattaca tttttctttt gtgataatcc tttataatac
      601 tgaactttaa cttgaaacga atcaaaatcc atctggtaaa aatgactaat tggctttttt
      661 tggcaaactt gaatcccaca ttcaatcgct aaagctgtag aaacattgtt taaaattacc
      721 aaatcaccta ttacattgga ttcaattttt tcatacattt gttccaatga tcccatatca
      781 actagcagaa tgagtccatt cgtcgtatct atacttttac aaaaattgtt gattaggtta
      841 attaagtcac tagtctcacc ttcaattggc atatcgaaag cctcataaac atatgtattc
      901 agcaacttat ttgtttccct tgcgatcgca gaagctgtta ttttaccgtg cataactatc
      961 agacaatggg tttctggtat tttcacatag aacttcttta caagaaaaag tatccatttc
     1021 aacaaaagtt cttgtttact ttcttcgact tctaaactac t
//
LOCUS       DAYYBG010000227         1059 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000227 DAYYBG010000000
VERSION     DAYYBG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.237"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..988
                     /locus_tag="ACHAUN_002960"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACHAUN_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          370 a          193 c          214 g          282 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
     1021 catccgagga ggctcattgt caaggacaat cggaatagc
//
LOCUS       DAYYBG010000228         1055 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000228 DAYYBG010000000
VERSION     DAYYBG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.239"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..289)
                     /locus_tag="ACHAUN_002961"
     CDS             complement(<1..289)
                     /locus_tag="ACHAUN_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461561.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQG"
     gene            complement(291..>1055)
                     /locus_tag="ACHAUN_002962"
     CDS             complement(291..>1055)
                     /locus_tag="ACHAUN_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8461562.1"
                     /translation="TLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMV
                     SILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDN
                     SEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHG
                     NMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFI
                     DEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          401 a          185 c          128 g          341 t
ORIGIN      
        1 ccccctgtat aaaaatttca tcataaacgg ataaatcatc cgagaaaaat tgattatggt
       61 tagttaacaa gttaatttta cgtttaaagg aagaaccgat tttccggtga ttcacaaaaa
      121 caacatcatt aaatagccct aattctttta tacgctcact atatcttcta gcgttttcaa
      181 attcagaata aataaataaa tctccttttg attctcggta aaaattttga taaacattta
      241 atatattaat caaatgtaaa ggagtccaag caatgaaagc tgtcttcata ttaattctcc
      301 ttttttaaat acaagtaaga aatgcttccc aaataatacc agaaaacaat tgaaatgaca
      361 tcgcgcttat tgaaaagtac atgagcctcc accatttcat ctataaaaat agatagtata
      421 aaaattaaaa caataccaag aaacgcataa ttcactttac ttctaaaaaa tgttttgagt
      481 aaactaaaga tatttaacac atagaaagca tagatacata taaaagcaat tataccactt
      541 gatacaagta cttgaagaaa tccattatgc atgtttccat gtgctctttt tagttcattc
      601 acatctgtag tatcaagatc tacttttttc aaaaattttg catcgctatt cccatagaaa
      661 tcggtctctc cataaccaaa taacaaagtt ggtcttataa ctttaagcgc agctttccaa
      721 atcgtaaaac gaccagctga aatttcggag ttatcactag aatgctgtaa tattacttct
      781 ttttctggaa gtggagtttt atctgattca tttgactcta taacttctac ttttctatca
      841 atcgatacag gaattgttgc taaaaaatcc ttagcataat tagtaccaaa agaaaaatat
      901 accaatagaa ctaataatat gcttaccatc catttgattt ttacaggtag tacttctcta
      961 atgctctttc taaattttga attggcaaac aacaaaagaa ataatactaa aaaagaacaa
     1021 attactacct gggcacctct agacgaagaa agtgt
//
LOCUS       DAYYBG010000229         1028 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000229 DAYYBG010000000
VERSION     DAYYBG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.240"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..877
                     /locus_tag="ACHAUN_002963"
     CDS             62..877
                     /locus_tag="ACHAUN_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461563.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
BASE COUNT          385 a          157 c          174 g          312 t
ORIGIN      
        1 gctggactac aagtaaaaaa tactgtatga tcatgacaga ttcaagaaag gagcaataag
       61 attggttaag atagcaggac tttctaaagg tggaaaaacc gtttcacaac tttataaaca
      121 aaactacaga gactggagac agttcagaaa aaacattaag gattcttact ttattctacc
      181 aacagaactt gaaaaatatt taccgaataa caaagctatt aatttatatc tgtattactg
      241 ttttaaggca aagaatgaag gcggggattc ctggcattcg gtcaatacca tagcagagtc
      301 tctaggagtg acaactaaaa ctataaacac ctggaataaa accttagttg atttaggtat
      361 gattgcacgt atagacgata accatctcag taaatcgaca attttacttc ctctctctag
      421 ttactataaa ttagcccttg atagttcttt aaaggatgtt accgagagca ctgtccatga
      481 aattgatggc aacttggttg cagtttatca tttgtttgaa tggagaaaaa atgaggctga
      541 tgaaaattat aacgttcctt ataacacact ctgttgtgtt tttaaaagaa aaacacaaaa
      601 agatactatc tataaattta tcttggtcca agatgagaaa ataagtaatt tcgaattaag
      661 cactccttct agtaagattt atgacgatgc atatagattt gaatgtcaag atctagaagc
      721 cctcctttcc gcaaataatc taaacaatat agaacatgaa aactttgttg taaacactag
      781 atttaattta gtttctgaaa aggactcaga tttgttagat ttgctgatag agcttactaa
      841 aaatctagat aagaaagaaa acattactgt actataacta gcatcggtcc atcattaagt
      901 ttggactatt agaacacttg ctattacaga aaaaggtatg ctagccacgc ttactattta
      961 ttggtatcaa acaagtatct taatttgaaa ggaagatgat ttatggctaa tttatatata
     1021 gagaacaa
//
LOCUS       DAYYBG010000230         1021 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000230 DAYYBG010000000
VERSION     DAYYBG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.241"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..>1021
                     /locus_tag="ACHAUN_002964"
     CDS             120..>1021
                     /locus_tag="ACHAUN_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461564.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKK"
BASE COUNT          347 a          126 c          137 g          411 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt taaaaaaaat aaatgatact atatgtaata taaaaaaaca
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      481 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      541 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      601 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      661 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      721 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      781 ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta
      841 tgattgagca gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa
      901 gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat
      961 ttggtattgg cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat
     1021 t
//
LOCUS       DAYYBG010000231         1005 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000231 DAYYBG010000000
VERSION     DAYYBG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.242"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..772
                     /locus_tag="ACHAUN_002965"
     CDS             <1..772
                     /locus_tag="ACHAUN_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8461565.1"
                     /translation="FSIIQTWIDLKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIK
                     NTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEI
                     GKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQG
                     IIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNV
                     YGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            762..>1005
                     /locus_tag="ACHAUN_002966"
     CDS             762..>1005
                     /locus_tag="ACHAUN_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8461566.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITE"
BASE COUNT          387 a          124 c          185 g          309 t
ORIGIN      
        1 gttttctatt attcaaactt ggatcgattt aaaatataat gccattacta cttggaataa
       61 tattaaacag tttttcaaag atacttggaa aaatattaaa gatacggcat acaatacgtg
      121 gatttctata aaaaatacca tgattaatac atggaataac attaaggact ctttctggaa
      181 tattgttact ggaattgtta attctgctga aaatgcatgg acaaatctaa aaaatggtgt
      241 ttcaaatgct attaatcggg taaaagaaat ctttaattcg ttaagggaag tcaatttatt
      301 tgaaattggc aagaacatca ttgatggact tataaatggt gtaaaagaaa agtggaatgc
      361 tttgaaagat actatcaaag gtattgcagg taatatcaaa aattggatta aaggagcttt
      421 agatattcat tctccatcaa gatggatgcg tgatatggtt ggtaaaaata ttgttcaagg
      481 tattatcgtt ggtattgata aagagcaaag taagttagat caaaccatga caaatttagt
      541 aaaaacaccg tctgttcaac ctgtagtaaa tggaataagt cacctaccaa ttacacaaac
      601 aaagcaaaat atatctgcta gtgaaacaaa agaaattcat ttacatttaa atgtttatgg
      661 agatttacct gattcaatgg ttcggaagat taccacaaaa attaaaacag aattgacaag
      721 acaaatgaaa cgagatgctg gtgcagtagg aggaacatta tatgcaactt aagagaggac
      781 aattttttat taatcaacat tattcttctg aatttaatgt gtatattcaa aacaggcctg
      841 cttctgtttc agctagtcga gtaattgaat tgagagagcg agaaggaaat gattctatca
      901 ttattgataa aggctattac aaaaatgtga ctagaaagat tgagtgttac tataaagcac
      961 cttcaattga tatggtgcag gagtgggaag atcgaatcac tgaat
//
LOCUS       DAYYBG010000232         1002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000232 DAYYBG010000000
VERSION     DAYYBG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.244"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1002
                     /locus_tag="ACHAUN_002967"
                     /pseudo
     CDS             369..1002
                     /locus_tag="ACHAUN_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAYYBG010000233          951 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000233 DAYYBG010000000
VERSION     DAYYBG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.246"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(196..594)
                     /locus_tag="ACHAUN_002968"
     CDS             complement(196..594)
                     /locus_tag="ACHAUN_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8461567.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(591..827)
                     /locus_tag="ACHAUN_002969"
     CDS             complement(591..827)
                     /locus_tag="ACHAUN_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8461568.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          303 a          167 c          129 g          352 t
ORIGIN      
        1 aatcagccca ctagttctaa accactattt cctactattc actcttttgt attcctttta
       61 aatcaattat ttcaaaatta atccatattt tccatatttt tatgaaaaaa ctattttgta
      121 tgattaaaca taaaaaaatc agcatattcc ttaatgacat gctgatctaa ttgagaaata
      181 aagtttttaa ttcgttcatt ttattgcggt ttgtcgtaaa taactggtta tcttggactt
      241 tagtgaatcg aaatccaatg aactagtggt aatcattatg ataaaatcaa cagcttcttg
      301 acgagaaaaa gcaactggac aattattcaa ttgaagaaac aacaacatgg caacaagagc
      361 agttcgttta ttgccattat gaaaaggatg ttttttagct aaattaattg ctaaaatcgc
      421 cgctttttca tagatttcag gatagagttc ttgattgaat actgcttgtg aaggctgttt
      481 gacggccata tctaatgcat ttgcatcttt tacaccgatc atttcgcctg gcgagtgacg
      541 taaaataact ttcgcgttga ttttgataat ttctttagtc gaaagatacc tcataaatca
      601 accaaatctt ttaacgcgtc ttcatattga ttcatgatcg aatcaactaa tgaatcaata
      661 tcaggttctt cttgtgcttt gatcattcgt acagaaccat cagattgaaa aacataactg
      721 attgcgtcac ctgtctgcac acccatgaat tcttttactt ctggtgggat agttgttaca
      781 tcgctattgc cagattttct aattttatac tttttcattt ttttcatatt tcgccaccct
      841 ctataccatt ctcattctat agtaagtaca ttatatccaa gtatatactt ttaatcaatg
      901 tatatacttt tattttgctt tttcttacga aaaaatgaca aagaaaaaag t
//
LOCUS       DAYYBG010000234          947 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000234 DAYYBG010000000
VERSION     DAYYBG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.247"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>947)
                     /locus_tag="ACHAUN_002970"
     CDS             complement(76..>947)
                     /locus_tag="ACHAUN_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461569.1"
                     /translation="KKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGE
                     KSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLI
                     RRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDK
                     FFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAG
                     KPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQ
                     IDDFPEEIIDRIE"
BASE COUNT          303 a          160 c          145 g          339 t
ORIGIN      
        1 tggaaagttt tgtctcgctt aatttgacaa ataatatttt tagaaaaagc agtcggactt
       61 tctaaaagaa gttcactatt cgatcctgtc gatgatttct tcaggaaaat cgtctatttg
      121 cttgtcaatt ttgctgtctt tgtatttctc ttcttggata gtcaacaaaa tctttgtaag
      181 attcccccag tctactgctc ttggcggatc gagccaaacc actacttgag atgcactttc
      241 ttcatataga ttattcctat tcgtgattat aacatccggg aactcaggct ttcctgcctc
      301 tatcacttcc gtgttcagat taatgtacaa gcttaaattt ttcttgataa attgattata
      361 ggcagcacca taagaaaaat cgatgatgat ctttactggt tcagaaatca gttgaatggg
      421 tatgtgattg atcaataata ataaataact gaaaaaaaga aaaaatttat cttgaagcaa
      481 ctctttgcgt tttgacgtta gatcatttat atactctatc aaatagcaat aaaatgctgg
      541 atagttttct ctaaaaaaag aaacatcaag tggaaattca tcagctttat taatagtgta
      601 atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg atcaattggc tcgtctcttc
      661 tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat cgttcattca accattcgat
      721 tgtttcatct tcattttttc tcaagtattt ttgatccaat ttatcatgaa gataaagcca
      781 agagagtgtc ttattgataa aaataacgga tttttctcct aatactcctt ttaagtggta
      841 aaatcctata tccgaaaaaa atagcgactg attgctagca atatctaaag aatcagcttg
      901 ttcagccaac aggttgattg ctaaaaagtg gaataatttc ttttttt
//
LOCUS       DAYYBG010000235          893 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000235 DAYYBG010000000
VERSION     DAYYBG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.248"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            252..875
                     /locus_tag="ACHAUN_002971"
     CDS             252..875
                     /locus_tag="ACHAUN_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8461570.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          328 a          129 c          167 g          269 t
ORIGIN      
        1 aactaatgtc cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt
       61 gatgtagcca gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt
      121 ttgggtatac tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt
      181 tttctttatt gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg
      241 aatgatggca aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc
      301 ggcaacataa gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa
      361 aaattttcga ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc
      421 ttatttcttt catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa
      481 gaaatagtaa agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg
      541 aaatattaga cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa
      601 caaatttagt catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag
      661 agcgtcaaaa acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga
      721 ttcaatacca tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgttgatg
      781 gtctgaagaa aaaagaaact atttctaaaa tagcaaagaa cgcaggagtt acaagaaaaa
      841 ccgtttatcg agtaaaaaga gatttaggaa tgtaatatgt tccagttaga aag
//
LOCUS       DAYYBG010000236          853 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000236 DAYYBG010000000
VERSION     DAYYBG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.250"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..251)
                     /locus_tag="ACHAUN_002972"
     CDS             complement(<1..251)
                     /locus_tag="ACHAUN_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461571.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLT"
     gene            complement(258..806)
                     /locus_tag="ACHAUN_002973"
     CDS             complement(258..806)
                     /locus_tag="ACHAUN_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8461572.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          286 a          177 c           85 g          305 t
ORIGIN      
        1 cttgttaaag aataattaaa cacttcattt ttacttgtta ttttttcatt gactataact
       61 agttcatctg gtaaaaaaat taaccaacaa acatcgacaa acagtaaatg atataatcca
      121 tttccacctc caagcatatt caatactggt gaacatgcct tagaaacacg aatatagtta
      181 tggtttaaat agatattttc tttaaacatt gcttgtttaa aagccatatg ctcttgttct
      241 aaacttttca taaactttta ctctaatgca cagctagcag cagctgctgc aattcctgct
      301 acaatattta ccttagctaa ctctttagct gcagcttttc ctaattgttt ttctgcaatt
      361 tttaataata aatccgcagc tttttcaaaa ttaaaagctt ttaatgcttc aataaaatct
      421 ccctgcgtac ctatagccca agccactcct cctccaggaa gagaattaat ggctacacat
      481 ccagcatatc gtttccaatc aactttctcc attctaggaa caataatatg ttcataaaag
      541 actcttgctg ctcttccttg ttcgttatct tgactgattc gttcacgaat agcaccaaaa
      601 tcctttatat ggtatacacc atgttcgtct aaaaaaccaa ctgtatcaaa ataaaaagtt
      661 aattcatcta ctaatttatt ctttatttct aaatcaactg tattcacttt ttctgtggca
      721 tacacacttg ttccagtact aacaaaagta cttaaaccta ataagaaaac acctaaaaca
      781 ccaaatattt taattattct tttcataatt tctcctccat aaaaaataag ttacttttta
      841 aatatcttgt tta
//
LOCUS       DAYYBG010000237          811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000237 DAYYBG010000000
VERSION     DAYYBG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.251"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACHAUN_002974"
     CDS             <1..672
                     /locus_tag="ACHAUN_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8461573.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAYYBG010000238          794 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000238 DAYYBG010000000
VERSION     DAYYBG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.252"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACHAUN_002975"
     CDS             85..624
                     /locus_tag="ACHAUN_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461574.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          309 a           97 c          146 g          242 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aatt
//
LOCUS       DAYYBG010000239          792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000239 DAYYBG010000000
VERSION     DAYYBG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.253"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>792
                     /locus_tag="ACHAUN_002976"
     CDS             <1..>792
                     /locus_tag="ACHAUN_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8461575.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNN"
BASE COUNT          269 a          137 c          153 g          233 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata at
//
LOCUS       DAYYBG010000240          748 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000240 DAYYBG010000000
VERSION     DAYYBG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.254"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(350..463)
                     /locus_tag="ACHAUN_002977"
                     /pseudo
     CDS             complement(350..463)
                     /locus_tag="ACHAUN_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          272 a          119 c          115 g          242 t
ORIGIN      
        1 catccatctg gaatttgtct ttttcgttgc tttgctcata aaaatctgca tattcatcac
       61 taatattttt tctaataaat tcttccatca taaagatcac ctcgaaaaga gtatacgaac
      121 aaacgttctt tttgtaaagg tggaatttat tatactaaat aaataggtga acaagtactt
      181 gtgccaaatt atgtgccaaa aaaaatcgaa tttaataaaa cacaaacaaa aagaaatcct
      241 attatgataa catttcttat aataacaaac acaacagaag acgtgtaata gttagtcagg
      301 aacgtacaaa taacccctgt atcctttgcg gtacaggggg ttattttgta taagtagaca
      361 ttgtagtttc taaactgcta tgtcctaaac gtttcgatac gctaagtata tttactcctt
      421 gataaagtaa aatagatgca tgcgtatgtc ttagtccatc aataaacagt cgaagagaaa
      481 atagggaaaa attgattact aaagaaaaac ttactaggtc gacggtgtag ttgattttca
      541 tacttcaata gattcgaatt aaacgatttc gattggctta taccagtcct atccaacgaa
      601 agatatatcg ttcttaactc tagcggtata acttgaagct ttacgtaaag ccaatgggaa
      661 tgaactggag atgacttttt attattttta gtaatagctg atgggttaat acttacaact
      721 ttccttttca aagtaccata taaaaaaa
//
LOCUS       DAYYBG010000241          724 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000241 DAYYBG010000000
VERSION     DAYYBG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.256"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..696
                     /locus_tag="ACHAUN_002978"
     CDS             28..696
                     /locus_tag="ACHAUN_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8461576.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          193 a          124 c          130 g          277 t
ORIGIN      
        1 aaattttcaa aagaaaagag gctagaaatg aaaaaattaa atttatttac agaattgtgc
       61 ggaatcattt taggcgcagg aatctacgga ttagctgtga caggaatcaa tattccatct
      121 aagctggcag atggtggggt tactggtatt gctttgcttt tgcataatct ttttggattc
      181 gctccttctg tcacttcact gctttttaat ttacctttat tgcttatctc attgtttctt
      241 tttggaaaac acgcttttct ccggacgatc gtcggaacat ttgcgctagt tttcttttta
      301 catttatggg agagccttca tatacatttt gctgtagata gtctactgat caatagtttg
      361 atgacaggta ttctttctgg cattggttgc ggtctcgtct ttcgattcgg cggaagcaca
      421 ggcggaacag atatcattta tcaagccatt gaaaaatatt ttcatattaa gattggcaaa
      481 agtcttttct tgattacttt tggtatttta gttgtctcct tgctttattt ggatctcaca
      541 cattttctgt atacattact tagctgttct attctatctt atacattgaa taaagtgaag
      601 tatctggaaa ttggatctgt tttgccccaa ttggaagcaa aacagactac agaaacattt
      661 gaaccacttg atcctcttga acagttagag cactgattct tatttttcac tactttattt
      721 aatt
//
LOCUS       DAYYBG010000242          720 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000242 DAYYBG010000000
VERSION     DAYYBG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.257"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>720
                     /locus_tag="ACHAUN_002979"
     CDS             82..>720
                     /locus_tag="ACHAUN_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFM8461577.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGC"
BASE COUNT          237 a          127 c          141 g          215 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
//
LOCUS       DAYYBG010000243          715 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000243 DAYYBG010000000
VERSION     DAYYBG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.258"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(497..>715)
                     /locus_tag="ACHAUN_002980"
     CDS             complement(497..>715)
                     /locus_tag="ACHAUN_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461578.1"
                     /translation="SDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRFIG
                     VDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          258 a           99 c          130 g          228 t
ORIGIN      
        1 tacttctttt ttaatttgtt cttgcaatct tttttgctac gtaatttttg taaaaaagtg
       61 ggttgagaga aatctaattg attttcaaag aaggagtatg aaaatgagaa aaatatttat
      121 tcgtttattg tgggtactat cttagaggaa taaataatag tggagaaaac tatggtttgg
      181 cgcagattct atcaggtttg atggaacgaa gacaacctcc cgacatcaag tcaaaagcta
      241 cttgagattt tgatctgaaa ataaaaaaag aaaacaaggt aggaacatca atgtttctac
      301 cttgttttcg taatcaatgg gaaaaaacta agagtggtgt acgtaatcaa taaaaaggat
      361 ggtgacaaca gtacctcttt actctctgaa agcatcataa ctcgtttttc ctcattatgc
      421 caataaattg ttgttatacc acatataagg atttgtaata gctaaaaaca gacaggaaag
      481 ttgaaatctg tgaatcttat tttgtatgga aggatttata gaacgcaaaa atcaagataa
      541 agatgaataa aaatcctgtt tcggctatat attgcgtatc tacgccaata aaacgagttt
      601 gttctgtcat agccatataa agcaaaggca aaaatacaac tacagcgaga taagatccga
      661 tgatttctcg aaaagttttc caggaagtat gtttcctgtt tttatattta tccga
//
LOCUS       DAYYBG010000244          713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000244 DAYYBG010000000
VERSION     DAYYBG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.259"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..443)
                     /locus_tag="ACHAUN_002981"
     CDS             complement(144..443)
                     /locus_tag="ACHAUN_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8461579.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          236 a          127 c           88 g          262 t
ORIGIN      
        1 attaaaggat aaaatacaat ataaagaaac atctgtttct ttatttagaa taaaaaaata
       61 cagactctgt tcaaataaat gaacctagcc tgcattatga gattgactat actttttaat
      121 tcattgagat gaataactcg tttttataaa tgcccaaaag taatcggtgc cgaagccatt
      181 cctcgaccta attctttttc aaattttcct attgttgata tctccatata aaacggtgat
      241 actggtttag acaattctcc cctgattgct aacggacgta aactacgttc caaattattt
      301 atgactttag gataatactg acctttttct aagcctgtct ttgcgtctat caaaagtata
      361 cgttcctttt ctttgatctc tggatttaaa acgagatcat ataattttag taccatgttt
      421 tctatttctt cacttttaga catcacgatt tcctcccaaa aatatatttc ttcccactac
      481 attctataaa caaagtatac gctacatcta gattttttta aatcattact ggattgttaa
      541 aacctttcaa tacactgtta tggcaacgat ttttatttta aaaacattct aaattaaatc
      601 agtaaaaatt tttattctaa gatttttatt agaattacgc ctaatttgtg tctctcaggg
      661 caattctaag aacgtttcaa gagatagcga cactatcgtt atcgaaaaag gcg
//
LOCUS       DAYYBG010000245          711 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000245 DAYYBG010000000
VERSION     DAYYBG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.260"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..>711
                     /locus_tag="ACHAUN_002982"
     CDS             290..>711
                     /locus_tag="ACHAUN_002982"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8461580.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEY"
BASE COUNT          268 a           79 c          118 g          246 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg a
//
LOCUS       DAYYBG010000246          709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000246 DAYYBG010000000
VERSION     DAYYBG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.261"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..600
                     /locus_tag="ACHAUN_002983"
     CDS             <1..600
                     /locus_tag="ACHAUN_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8461581.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          180 g          189 t
ORIGIN      
        1 accatgaaaa aagaatttcc gatcccgatt tcttggacaa cagatgtcaa tgcggctgcc
       61 tatggtgagt atgtcttcgg cagtggcaaa ggattgagca gtgtcgttta ctatacgatc
      121 ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct ttatcgaagg gtttagtcat
      181 ccagaaatgg ggcatatgct cgtcgtccct catccaaaag atgactttgc tggcagctgt
      241 ccatttcatg gcaactgttt agaagggatg gccgctggac ccgccatcga aaaacgctta
      301 gggaaaaaag gacaagaggt tgcagaagat gatccttatt gggaaatcga agcctcctat
      361 attgctcaat gtgcctacaa tacaaccttg atgttttctc cagatgtgat catttttggc
      421 ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc acgacgcatt tgcaaaattg
      481 gtcaatgggt atgtcgaaac acctgagtta agcaaatata tcgtgacccc agcattagaa
      541 gacaacgctg gaacgctcgg ttgtttggca ttggctcgag aagcggtctt acattcataa
      601 acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa gatacaactg aaaatttagt
      661 cataaccagt taggcagagt gcttaactgg tttttgtttt acttggaag
//
LOCUS       DAYYBG010000247          691 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000247 DAYYBG010000000
VERSION     DAYYBG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.262"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACHAUN_002984"
     CDS             complement(<1..461)
                     /locus_tag="ACHAUN_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8461582.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          220 a          126 c           98 g          247 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga c
//
LOCUS       DAYYBG010000248          679 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000248 DAYYBG010000000
VERSION     DAYYBG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.263"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>679
                     /locus_tag="ACHAUN_002985"
     CDS             <1..>679
                     /locus_tag="ACHAUN_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8461583.1"
                     /translation="NTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELT
                     LEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQ
                     TMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGT
                     KEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQH
                     CCIHVSR"
BASE COUNT          241 a          127 c          117 g          194 t
ORIGIN      
        1 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
       61 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      121 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      181 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      241 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      301 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      361 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa
      421 aacgtcaaac cgtatccaaa gaagcgattt atattgccat tggtatacga gaagacggca
      481 ctaaagaagt actgagttat gcgattgctc caactgaatc aacatacgtt tggaatgagc
      541 tgctacagga tattaactcc agaggagttc aagaagtctt gctttttatt acggacggct
      601 taaaaggcat gaaagatact atccatcaaa tttatcctaa agcaaaatat cagcattgtt
      661 gtatccatgt atctcgtaa
//
LOCUS       DAYYBG010000249          656 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000249 DAYYBG010000000
VERSION     DAYYBG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.264"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACHAUN_002986"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACHAUN_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8461584.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAYYBG010000250          635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000250 DAYYBG010000000
VERSION     DAYYBG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.265"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..609
                     /gene="spxA"
                     /locus_tag="ACHAUN_002987"
     CDS             211..609
                     /gene="spxA"
                     /locus_tag="ACHAUN_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8461585.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          227 a           95 c          120 g          193 t
ORIGIN      
        1 atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt tactgtatat
       61 taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag ctatttaaaa
      121 gttgattatg tataatagaa acagatggga gattattatg atcttctgtt acaaaaaagt
      181 gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc cccaagttgt
      241 acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt tgttgaacgt
      301 aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca aatgacagaa
      361 gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact gaacatggat
      421 ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc tggattgctt
      481 cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga agatgaaatt
      541 cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca attgatggct
      601 ggattataga aggataatac gaaacttgga aaaga
//
LOCUS       DAYYBG010000251          593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000251 DAYYBG010000000
VERSION     DAYYBG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.266"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..580
                     /locus_tag="ACHAUN_002988"
     CDS             <1..580
                     /locus_tag="ACHAUN_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8461586.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DAYYBG010000252          582 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000252 DAYYBG010000000
VERSION     DAYYBG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.267"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>582)
                     /locus_tag="ACHAUN_002989"
     CDS             complement(37..>582)
                     /locus_tag="ACHAUN_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8461587.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          166 a          110 c          100 g          206 t
ORIGIN      
        1 aatccactcc ttttttcatg ctctaaaatg atatttttac acaactgtta cggttgtttt
       61 acgaaattgg acgggacttt gccagtttaa tttctgtttt atccgttcat tattgtagta
      121 atagatatag ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg
      181 aaaaatctct tgttttaata agccaaaaaa attctccata agtgaattat ctaagcagtt
      241 ccctttccga gacatgcttt gaaagatttt gtactccttc aatttgtttc tataagcttt
      301 catttgatac gcccaacctt gatctgagtg aaacgtacga cgatagggac aatcgttagt
      361 ggtctggata gcttcctcca aaccttccat taccgctaat gcattgggct tttcagagat
      421 acgataggat aaaatctcac tattgaacat atccataaaa ggatctagat acaatttctt
      481 ttgacggata atgcctttgg catccacttc atggtattta aattcagttg tatccgtcgt
      541 gattttctga tggcagatac tggtgtagaa acgacgatgg at
//
LOCUS       DAYYBG010000253          582 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000253 DAYYBG010000000
VERSION     DAYYBG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.268"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..296
                     /locus_tag="ACHAUN_002990"
     CDS             <1..296
                     /locus_tag="ACHAUN_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461588.1"
                     /translation="PTALSFSVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSV
                     SLPPQVNDINFNINARNVLVEEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            310..>582
                     /locus_tag="ACHAUN_002991"
     CDS             310..>582
                     /locus_tag="ACHAUN_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461589.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNL"
BASE COUNT          219 a           77 c          112 g          174 t
ORIGIN      
        1 ttcctactgc tttatcattt tctgtaacag ctgtggtgtc tgagatagag gatacagaaa
       61 agataaaaat agtagaaatt gaagttttaa acaagaatga aaaacaaatt ttttctacgg
      121 gtgaagtatc ggtaagcttg cctcctcaag ttaacgatat aaactttaat ataaacgcca
      181 gaaatgtctt ggttgaagaa gcaggagagc attatgctgt tgttaaattt aatggaactg
      241 agattggaag gcaaatcttt gatatcaagg tcaacaaacc agtggaaaaa aattaaggag
      301 acgatacgga tgcttgatat agtaccatca aatacacatc ggagtggtag cttaataaat
      361 tttgctgcga tttttgcttg cgcatcggta attctagcat ctccagttgt tgtggatgca
      421 agttctactc cgaaaactaa aaatgataat caacttgtaa ttactacaaa aaatgagatt
      481 aacacaattt caagtaatga tggcaaaatt tttgatgtga taaatacggt tttaaaaaga
      541 aaagctgaaa tcaatataaa atatgatgaa gatttaaact tg
//
LOCUS       DAYYBG010000254          543 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000254 DAYYBG010000000
VERSION     DAYYBG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.272"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            215..529
                     /locus_tag="ACHAUN_002992"
     CDS             215..529
                     /locus_tag="ACHAUN_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461590.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          162 a           75 c           85 g          221 t
ORIGIN      
        1 agtgtgttta tcgctttttc taagtgcgaa cggaataatt catttaaatc gcctttagtg
       61 actagagttt gcacaatttc tgtagtaaaa tcattcatag ggaagtcctc ttttctgtga
      121 attggttgtc gttaacttta ttctacagaa gggacttcct tttttgtatg gattttttca
      181 tttacacaaa atattttata ctctcggata gcttttggat ttttacagta attttatctt
      241 aataatagca atattattac ttctaaatat ttggtttttt gataagagta gaaatgcagg
      301 gataggtttt agaaccaaac gaagtacgtc ttctgaaaaa aagtgggtat attcacagac
      361 tattttttac ggaggggtta tttcaatcag tttactttct tcaacacttt attcttttaa
      421 tgttatagac gtttccatgt caaattttat atctattatt ggaatcctta tttctgcaat
      481 tattacacaa ctacttcttg tgtttgaaga gaagtcaaaa aataattaaa gcaattatgc
      541 ctt
//
LOCUS       DAYYBG010000255          529 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000255 DAYYBG010000000
VERSION     DAYYBG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.274"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..350)
                     /locus_tag="ACHAUN_002993"
     CDS             complement(48..350)
                     /locus_tag="ACHAUN_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8461591.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          181 a           92 c           74 g          182 t
ORIGIN      
        1 tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta atctttatac
       61 ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc gctccgtgcg
      121 tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat tctggttttt
      181 gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa taagctatct
      241 tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta
      301 ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat aatgttcacc
      361 tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat tgataatatg
      421 gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg ccattttcat
      481 tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaa
//
LOCUS       DAYYBG010000256          526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000256 DAYYBG010000000
VERSION     DAYYBG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.275"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..354
                     /locus_tag="ACHAUN_002994"
     CDS             <1..354
                     /locus_tag="ACHAUN_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8461592.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAYYBG010000257          522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000257 DAYYBG010000000
VERSION     DAYYBG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.276"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>522
                     /locus_tag="ACHAUN_002995"
     CDS             <1..>522
                     /locus_tag="ACHAUN_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8461593.1"
                     /translation="VDYIKEKVTLGWTPDVIIGRKERSVSCGMRTLYRLFSKGIFDID
                     TLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVI
                     TLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISN
                     QHDIDIYFADPGTP"
BASE COUNT          172 a           98 c           98 g          154 t
ORIGIN      
        1 agtagattac attaaagaga aagtcactct tggttggacg cctgacgtca ttatcgggcg
       61 aaaagaaagg tctgtttcat gcggtatgag aacactttat cgtttatttt ctaaaggaat
      121 atttgatatt gacacactac cgatgaaagg taaaagaaaa cccaatggcc atcaggaaaa
      181 acggggaaaa caacagtatc agcgctcaat ccatgatcga cctgataatt atcctgattt
      241 caattctgag tttggtcacc ttgaaggtga tacgatcgtt ggcattcatc ataaaagtgc
      301 cgtcattact ttagttgaaa gattatctaa agtcattatc acgattaaac ccaacggccg
      361 taaggcatta gatattgaaa ctgcccttaa tcaatggttt tctcgcttcc ctaaaaactt
      421 ctttaaatct attacgtttg actgtggaaa agaattttct aactggaaag ccattagtaa
      481 ccaacatgat attgatatat attttgcgga ccctggaaca cc
//
LOCUS       DAYYBG010000258          514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000258 DAYYBG010000000
VERSION     DAYYBG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.277"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHAUN_002996"
     CDS             complement(<1..132)
                     /locus_tag="ACHAUN_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8461594.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAYYBG010000259          511 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000259 DAYYBG010000000
VERSION     DAYYBG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.278"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..161)
                     /locus_tag="ACHAUN_002997"
     tRNA            complement(88..161)
                     /locus_tag="ACHAUN_002997"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(125..127),aa:Ile,seq:cat)
     gene            complement(328..>511)
                     /locus_tag="ACHAUN_002998"
     CDS             complement(328..>511)
                     /locus_tag="ACHAUN_002998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461595.1"
                     /translation="IKVHLKKSVRTKVLFVNLKIDFPEYVLGISKCLNVFLNAKSMQK
                     ILYFSTFLSLTKIKKR"
BASE COUNT          174 a           83 c           97 g          157 t
ORIGIN      
        1 catttgagtg gcaatgaaga aaagctttag cttgtataat actcttcaaa aaattctaac
       61 acagaacgat tcaattggct acattaatgt accctgtagg actcgaacct acgaccggac
      121 ggttatgagc cgtctgctct gaccaactga gctaagggta gtggttgttg ccacataaag
      181 ccataaacaa tcaaccagta gaatgcgtgg caacaaacct gttatcgcat atcttggagt
      241 gtgactattt atgagtgata gtgaagatat gcgtctccat atatgtatgg ttgagacaga
      301 taaacatgta tgaaaaacgt tgttaaatca acgttttttt attttggtca aggagagaaa
      361 agtagagaaa tacaaaattt tttgcattga ttttgcattc aaaaaaacat tcaaacattt
      421 agaaatcccc aatacgtatt caggaaaatc tattttaagg tttacaaaaa gaacttttgt
      481 tcgtacactc tttttgaggt gaacttttat g
//
LOCUS       DAYYBG010000260          508 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000260 DAYYBG010000000
VERSION     DAYYBG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.279"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DAYYBG010000261          507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000261 DAYYBG010000000
VERSION     DAYYBG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.280"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..351
                     /locus_tag="ACHAUN_002999"
     CDS             64..351
                     /locus_tag="ACHAUN_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461596.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          188 a           74 c           87 g          158 t
ORIGIN      
        1 aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga
       61 aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat
      121 caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat
      181 aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac
      241 atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa
      301 ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata
      361 agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg
      421 tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt
      481 tctactttat tggctgtttc tttttat
//
LOCUS       DAYYBG010000262          495 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000262 DAYYBG010000000
VERSION     DAYYBG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.281"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..165
                     /locus_tag="ACHAUN_003000"
     CDS             43..165
                     /locus_tag="ACHAUN_003000"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461597.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            138..269
                     /locus_tag="ACHAUN_003001"
     CDS             138..269
                     /locus_tag="ACHAUN_003001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461598.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          185 a           73 c           87 g          150 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catcgccgat tgtaaaatta
      361 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      421 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      481 gtcttatttc aaaat
//
LOCUS       DAYYBG010000263          494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000263 DAYYBG010000000
VERSION     DAYYBG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.282"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..326
                     /locus_tag="ACHAUN_003002"
     CDS             <1..326
                     /locus_tag="ACHAUN_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461599.1"
                     /translation="KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQR
                     EGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
BASE COUNT          198 a           66 c           90 g          140 t
ORIGIN      
        1 aaaaggcaag tttaaaatca ctaaaagata catggcttga tttatcagaa gataatccat
       61 acagccaatt cttaattact gtaatggctg gtgttaacca attagagcga gatcttattc
      121 ggatgagaca acgtgaaggg attgaattgg ctaagaaaga aggaaagttt aaaggtcgat
      181 taaagaagta tcataaaaat cacgcaggaa tgaattatgc ggtaaagcta tataaagaag
      241 gaaatatgac tgtaaatcaa atttgtgaaa ttactaatgt atctagggct tcattataca
      301 ggaaattatc agaagtgaat aattagccat tctgtattcc gctaatgggc aatattttta
      361 aagaagaaaa ggaaactata aaatattaac agcctcctag cgatgccgaa aagccctttg
      421 ataaaaaaag aatcatcatc ttaagaaatt cttagtcatt tattatgtaa atgcttataa
      481 attcggccct ataa
//
LOCUS       DAYYBG010000264          479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000264 DAYYBG010000000
VERSION     DAYYBG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.284"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACHAUN_003003"
     CDS             complement(<1..339)
                     /locus_tag="ACHAUN_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461600.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..>479)
                     /locus_tag="ACHAUN_003004"
     CDS             complement(356..>479)
                     /locus_tag="ACHAUN_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8461601.1"
                     /translation="VKQFLWKEMFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          140 a           82 c           77 g          180 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat tgtttgact
//
LOCUS       DAYYBG010000265          476 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000265 DAYYBG010000000
VERSION     DAYYBG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.285"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>476
                     /locus_tag="ACHAUN_003005"
     CDS             22..>476
                     /locus_tag="ACHAUN_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8461602.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DAYYBG010000266          476 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000266 DAYYBG010000000
VERSION     DAYYBG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.286"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>476
                     /locus_tag="ACHAUN_003006"
     CDS             <1..>476
                     /locus_tag="ACHAUN_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8461603.1"
                     /translation="IIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSS
                     NLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKK
                     SYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          157 a           86 c           85 g          148 t
ORIGIN      
        1 gattattgct tgtgttatat ggggcataat taatggctat actagccaaa gagccacgta
       61 tagagcggtt tgttccgtct tatttcctaa tggtgacttt cctagtagga gtcgattcac
      121 tcgcttaagt tcaaacttag cttacactat caagattatt cgatactttt tcatcaagaa
      181 gctcaccaaa ggtgaactag tcggaattat agatagtttt cctagtccat tatgtaaacc
      241 agttagaaat agacaagcaa aactattgaa tcaaatagcc aaagttggct ataactcaac
      301 aaaaaaatct tatttttatg gtcttaaaat tcatatgatc gttactaaga ctggctttcc
      361 gattacttac tcaatcacga atccaggtgt ccatgacgtg aaagtgttag agactttatc
      421 ggaagaagcg aatcttccca atatcctagg ggacaaagga tatattagcc ataaaa
//
LOCUS       DAYYBG010000267          460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000267 DAYYBG010000000
VERSION     DAYYBG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.289"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHAUN_003007"
     tRNA            complement(20..91)
                     /locus_tag="ACHAUN_003007"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHAUN_003008"
     tRNA            complement(113..200)
                     /locus_tag="ACHAUN_003008"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHAUN_003009"
     tRNA            complement(225..298)
                     /locus_tag="ACHAUN_003009"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHAUN_003010"
     tRNA            complement(312..382)
                     /locus_tag="ACHAUN_003010"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHAUN_003011"
     tRNA            complement(384..459)
                     /locus_tag="ACHAUN_003011"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAYYBG010000268          433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000268 DAYYBG010000000
VERSION     DAYYBG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.291"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          149 a           72 c           66 g          146 t
ORIGIN      
        1 tactttgatt tttaaataga aagacatttg ggttaaattg tgagataata ataaagaaga
       61 gtttaaagcg caccccaaac cacttcccca taagtgtgtt acgctttaaa ctcttttata
      121 tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttacaa
      181 ttggagtggt agttaattag tgacttattt ttgattttat agcactgata ctataaaata
      241 tagatatcat catattacac aatcttaata ctaacttaaa aaatatctcc tttcacaagt
      301 atggtgataa aattcgttcc gggctacctt tttaggtagc ctactttaat ctttatacct
      361 ttctggatca acgaaagtat actttatata gtcataacgc cgatgatcgc tacgtgcgtc
      421 cggcacgtca gtc
//
LOCUS       DAYYBG010000269          411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000269 DAYYBG010000000
VERSION     DAYYBG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.292"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACHAUN_003012"
     rRNA            <1..>411
                     /locus_tag="ACHAUN_003012"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAYYBG010000270          388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000270 DAYYBG010000000
VERSION     DAYYBG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.294"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..>388
                     /locus_tag="ACHAUN_003013"
     CDS             146..>388
                     /locus_tag="ACHAUN_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8461604.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT          141 a           62 c           74 g          111 t
ORIGIN      
        1 attagaattt taaaaaatga taaaaattca aaaagaaaaa atagtagtta tttacttgtc
       61 atgttactaa ataaaaaaat aatttaaata tgatatatag tcatttattt agtgatttca
      121 ttctaaaata aagggagaaa tgtctatgct atacggtgca attgaagcag gcggcacaaa
      181 atttgtgtgt gccattggcg atgaagaaat gacgatcaag gaaagagtga gctttccgac
      241 aacgacacct gaggaaacaa tgcctttagt cattgacttc ttcaaacaat accaagcaga
      301 tttagcaggg attgggattg gctcatttgg gcctatcgat atccatcgcg attccgccac
      361 ttatgggtac attacctcga ccccgaaa
//
LOCUS       DAYYBG010000271          386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000271 DAYYBG010000000
VERSION     DAYYBG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.295"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACHAUN_003014"
     CDS             <1..>386
                     /locus_tag="ACHAUN_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8461605.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAYYBG010000272          386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000272 DAYYBG010000000
VERSION     DAYYBG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.296"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACHAUN_003015"
     CDS             <1..>386
                     /locus_tag="ACHAUN_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8461606.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAYYBG010000273          369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000273 DAYYBG010000000
VERSION     DAYYBG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.299"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..>369
                     /locus_tag="ACHAUN_003016"
     CDS             98..>369
                     /locus_tag="ACHAUN_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461607.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          139 a           52 c           70 g          108 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttt
//
LOCUS       DAYYBG010000274          362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000274 DAYYBG010000000
VERSION     DAYYBG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.300"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACHAUN_003017"
     rRNA            complement(<1..>362)
                     /locus_tag="ACHAUN_003017"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAYYBG010000275          344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000275 DAYYBG010000000
VERSION     DAYYBG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.301"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACHAUN_003018"
     CDS             <1..>344
                     /locus_tag="ACHAUN_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8461608.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAYYBG010000276          341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000276 DAYYBG010000000
VERSION     DAYYBG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.denovo.302"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACHAUN_003019"
     CDS             <1..>341
                     /locus_tag="ACHAUN_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8461609.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAYYBG010000277        21767 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.guided.046, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000277 DAYYBG010000000
VERSION     DAYYBG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.guided.046"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1508)
                     /gene="whiA"
                     /locus_tag="ACHAUN_003020"
     CDS             complement(573..1508)
                     /gene="whiA"
                     /locus_tag="ACHAUN_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8461610.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1542..2540)
                     /gene="yvcK"
                     /locus_tag="ACHAUN_003021"
     CDS             complement(1542..2540)
                     /gene="yvcK"
                     /locus_tag="ACHAUN_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8461611.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2537..3421)
                     /gene="rapZ"
                     /locus_tag="ACHAUN_003022"
     CDS             complement(2537..3421)
                     /gene="rapZ"
                     /locus_tag="ACHAUN_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8461612.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3556..4287)
                     /locus_tag="ACHAUN_003023"
     CDS             complement(3556..4287)
                     /locus_tag="ACHAUN_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8461613.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(4291..5556)
                     /locus_tag="ACHAUN_003024"
     CDS             complement(4291..5556)
                     /locus_tag="ACHAUN_003024"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8461614.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(5819..8635)
                     /gene="uvrA"
                     /locus_tag="ACHAUN_003025"
     CDS             complement(5819..8635)
                     /gene="uvrA"
                     /locus_tag="ACHAUN_003025"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8461615.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(8645..10639)
                     /gene="uvrB"
                     /locus_tag="ACHAUN_003026"
     CDS             complement(8645..10639)
                     /gene="uvrB"
                     /locus_tag="ACHAUN_003026"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8461616.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            10898..12400
                     /locus_tag="ACHAUN_003027"
     CDS             10898..12400
                     /locus_tag="ACHAUN_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8461617.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            12402..13139
                     /locus_tag="ACHAUN_003028"
     CDS             12402..13139
                     /locus_tag="ACHAUN_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8461618.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(13310..14503)
                     /locus_tag="ACHAUN_003029"
     CDS             complement(13310..14503)
                     /locus_tag="ACHAUN_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8461619.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(14582..14788)
                     /locus_tag="ACHAUN_003030"
     CDS             complement(14582..14788)
                     /locus_tag="ACHAUN_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8461620.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(14781..15923)
                     /locus_tag="ACHAUN_003031"
     CDS             complement(14781..15923)
                     /locus_tag="ACHAUN_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8461621.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            16435..17070
                     /locus_tag="ACHAUN_003032"
     CDS             16435..17070
                     /locus_tag="ACHAUN_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8461622.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            17224..17301
                     /locus_tag="ACHAUN_003033"
                     /pseudo
     CDS             17224..17301
                     /locus_tag="ACHAUN_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            17615..17662
                     /locus_tag="ACHAUN_003034"
                     /pseudo
     CDS             17615..17662
                     /locus_tag="ACHAUN_003034"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17678..19597
                     /gene="tet(M)"
                     /locus_tag="ACHAUN_003035"
     CDS             17678..19597
                     /gene="tet(M)"
                     /locus_tag="ACHAUN_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFM8461623.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            19716..19883
                     /locus_tag="ACHAUN_003036"
     CDS             19716..19883
                     /locus_tag="ACHAUN_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8461624.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(19943..20296)
                     /locus_tag="ACHAUN_003037"
     CDS             complement(19943..20296)
                     /locus_tag="ACHAUN_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461625.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            20800..21219
                     /locus_tag="ACHAUN_003038"
     CDS             20800..21219
                     /locus_tag="ACHAUN_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8461626.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            21245..21451
                     /locus_tag="ACHAUN_003039"
     CDS             21245..21451
                     /locus_tag="ACHAUN_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461627.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         6406 a         4366 c         3953 g         7042 t
ORIGIN      
        1 gcacattttt tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat
       61 agttcaagtt caggtataac gtataccaac catacatccc aattactatt tatgaactta
      121 tgattatcac tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct
      181 ggttgttttc gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga
      241 gaatcttgac cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt
      301 ttttaattaa tctccagtta tagataaata cttgatccta tctatatcag gacttctata
      361 ttaagcaaaa gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat
      421 acgattgtat taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga
      481 attatagttt gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat
      541 ctcctgcttc atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc
      601 gcaaattcat ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag
      661 ggaatcattt cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg
      721 atttcctgaa gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg
      781 ttttcgattt gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg
      841 actagacgat tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc
      901 attgagttgg ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa
      961 tacgaaatat agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac
     1021 atatcgcaaa tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga
     1081 ctcgtttccg gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc
     1141 attttttgcg aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc
     1201 atgatatcaa gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca
     1261 ttgttctttt ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga
     1321 tctttcaaaa gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa
     1381 ataaactgct ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc
     1441 gctttcgcat gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca
     1501 aatgacatcc tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa
     1561 actaaacgga acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc
     1621 aattctaaaa agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg
     1681 tgtgcgactt gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact
     1741 ttttccgtat tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca
     1801 cgtatatgat cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag
     1861 atatagacgg tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa
     1921 tttggaagga tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata
     1981 attgctgaaa cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca
     2041 tacacacggt caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt
     2101 ccgtctttaa aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca
     2161 tctacatgca tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg
     2221 gaaactgcgg caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt
     2281 ttgaaacggt attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag
     2341 acatttctca agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt
     2401 ccaccatcat ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt
     2461 ttcaggataa ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc
     2521 cggtaggttt tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat
     2581 gtgtcacatt gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca
     2641 cagaacggtg ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt
     2701 taatgtatcc aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg
     2761 tttcatcaaa ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta
     2821 gttccgggat ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga
     2881 tcggcaaccc gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct
     2941 ttgatttgaa tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat
     3001 caatgatcac ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc
     3061 cttcagtgat caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag
     3121 ccactagttc ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat
     3181 tttcgatttc gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca
     3241 ctcctaaagc catttttgtg actttacctg attctttgat caattcccag aattttggaa
     3301 tcaatcgagg cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag
     3361 ctactgtttt ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca
     3421 tatttacgaa cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt
     3481 accagttttc tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc
     3541 ccaaatccaa tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa
     3601 tgcacgttcg atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt
     3661 atcttcagca aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca
     3721 gtttaggcgc tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc
     3781 cgattccttg atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg
     3841 aatcactgtt tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact
     3901 tccttctgtt cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc
     3961 tgcttctctt ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa
     4021 gtagtgtgcg gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc
     4081 aatatcatcc agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt
     4141 acgatcagga actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt
     4201 agctttcgtt cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc
     4261 gcctaaaata ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt
     4321 gaatcttgga atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg
     4381 gtctttttca taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag
     4441 ttttttgtct tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca
     4501 taatcttgct ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa
     4561 cactcgatct tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact
     4621 gctacgacct tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata
     4681 cttcatatcg aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt
     4741 ttgatagatt ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg
     4801 tgtaggatct tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc
     4861 tacatacgca tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat
     4921 catttcactt ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct
     4981 agtctctaaa ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact
     5041 gtttgcaggt ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt
     5101 tactaacatt tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca
     5161 aacttcataa aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg
     5221 acagataaaa tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc
     5281 accacaagca atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg
     5341 aagcatcgtc tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac
     5401 aattttactg tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc
     5461 gtatgcttca ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat
     5521 agggataaat tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc
     5581 ttgtttttct tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag
     5641 gaaagtcctc cgattgctca attctttttt gaacggattg aacttatagg aagtttttat
     5701 ctataccaat cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata
     5761 aaacaagcaa aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt
     5821 ataccattat ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt
     5881 ctggtgttcc agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa
     5941 tgatataatc tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac
     6001 cagcatctac taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc
     6061 ctgtcgttgg ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac
     6121 tggcaagttt catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg
     6181 tcacataacc taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt
     6241 gcttgaagaa ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc
     6301 ctttgtaatg cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag
     6361 gaacatagac atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag
     6421 cttcacaacg accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt
     6481 ttgcctcgtt cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg
     6541 ctggattact tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga
     6601 tactttcgta gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt
     6661 tttgcgctaa cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca
     6721 cacctgtgac tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt
     6781 tttctgaagc accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta
     6841 cagggattga ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta
     6901 cttcttcagg agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga
     6961 cgtcaatcaa ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg
     7021 tgttgccaag atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat
     7081 gcaatccgat cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt
     7141 gtgtagccaa tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca
     7201 aagttagata gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta
     7261 aaatcggttt ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta
     7321 ctgctttttg aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag
     7381 atagagcttg gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat
     7441 aaagacgcat ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat
     7501 tcttcaatac cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat
     7561 aatggaaatg gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta
     7621 attcttcgaa tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt
     7681 gaggataata ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct
     7741 tcgttctatc tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac
     7801 agtctggaca agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa
     7861 cagtaaatcc gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga
     7921 tcacgtcaac cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata
     7981 ggcgagaacg aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt
     8041 ttttgttttt ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa
     8101 cgcggacgta tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct
     8161 ttaccacgat cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat
     8221 ctgccatttg atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat
     8281 gtcctacacg tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg
     8341 tcgaccgagg atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt
     8401 cgatgctatc tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa
     8461 ggctctctac gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt
     8521 ttccagaacc ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta
     8581 tatttttcaa gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt
     8641 cctcctactt tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa
     8701 aatccaaagc ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt
     8761 tttcttcctt cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg
     8821 attctttcgt aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc
     8881 catgttcttc attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta
     8941 gacgcataga atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag
     9001 cagctcgtcc aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg
     9061 catccaagat agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga
     9121 ttccaactaa cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca
     9181 gcgttttgat atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg
     9241 tgaggtcttc tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga
     9301 ctcgttcatt tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga
     9361 tcactgggtc aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt
     9421 catgttcata tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa
     9481 actcttctaa ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta
     9541 ctagcatctg ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta
     9601 cgtgcgattc gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag
     9661 gcggttcacc ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt
     9721 agcccatttc taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt
     9781 ccagaagttt gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat
     9841 tcgctacggc atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg
     9901 ctacgtgttc cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt
     9961 cgacctcatc tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa
    10021 tctccacgac atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt
    10081 cgaattggat actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc
    10141 gaatcgaaac tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa
    10201 cggatgcaat gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta
    10261 atttgtcgat ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca
    10321 cataagcttc aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag
    10381 ggaaaaattc cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa
    10441 tcagtgttgg tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc
    10501 cagtcgcacc taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt
    10561 taatcgcttc aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg
    10621 aggtatctct ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac
    10681 caataaaaag aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca
    10741 tactttcgcc acttttttat cttattatac atgttatgtt ttataacata aaatgttaga
    10801 aactcgcatt tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc
    10861 tataataata aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca
    10921 ctcatttgtt ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc
    10981 tgacaacact acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa
    11041 aaaagacgta tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc
    11101 aaagggcgag tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg
    11161 atttaacatc cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg
    11221 acaagcagac ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga
    11281 tttttctgat tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc
    11341 gatcaagtct tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag
    11401 tgctgaattt ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc
    11461 tgctgatcaa ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta
    11521 tccggtaatc ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga
    11581 atctggcgga aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat
    11641 gttcaatgaa ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga
    11701 atatgtagcg gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt
    11761 tcttggactt atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct
    11821 cctaactttg atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag
    11881 tgtagcacca atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg
    11941 aattccaatg atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt
    12001 tacgattcct gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat
    12061 cgcagagatt gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag
    12121 aagtttaggt ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg
    12181 catcatgatc ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat
    12241 ttccgcaatc ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac
    12301 acaaagtacg tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt
    12361 gacaaaatta gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag
    12421 ttgagcattt agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca
    12481 tcaacgaagg agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct
    12541 tgcgttgctt gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc
    12601 acttgatgga taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc
    12661 aacactttaa cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg
    12721 acctgaagaa agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag
    12781 ttggtctaga agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc
    12841 gtgtggctat tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta
    12901 cttctgcact cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag
    12961 aacaaggtat gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca
    13021 atcgcgtcat gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct
    13081 ttgaaaatcc gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa
    13141 ataaattata tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct
    13201 tattttatag gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat
    13261 cttcttatat tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta
    13321 gacgctgaac ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga
    13381 tactgatatt tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg
    13441 ccagtcttgt gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt
    13501 ccttgtggtg cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg
    13561 caactttggg cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa
    13621 atggttctgc ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc
    13681 ttaatggtac ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt
    13741 ccttgctctt tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg
    13801 ctactgtcag tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat
    13861 catcataatg cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct
    13921 gttcctctgt caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga
    13981 aatcaaaggg atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta
    14041 acgagcgttt atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg
    14101 cccattcctt agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct
    14161 ctttcaataa ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc
    14221 tctgtgcgtt ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga
    14281 taccgtcctc aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc
    14341 gttttccctt tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt
    14401 ttccaaatgc gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag
    14461 tatgcaggat acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc
    14521 ttgttggaaa gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc
    14581 attagatagc gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc
    14641 ggttgccatt cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact
    14701 tgttttctcc gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc
    14761 atacgggaat atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct
    14821 atgtccgtac acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca
    14881 tcaatcattt tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt
    14941 gacggcatga cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg
    15001 ttcatttcaa aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga
    15061 aattgcacaa aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac
    15121 aattcctcaa ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata
    15181 atatccgtat gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat
    15241 tttgtgcttg cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt
    15301 agttttccac cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg
    15361 tatttttcag acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca
    15421 aaacgtcgga taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg
    15481 ttctgtgcct gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat
    15541 acgcccatgt gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct
    15601 ttatagccat aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc
    15661 tttcggataa tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc
    15721 agataaaaag gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt
    15781 tccttgctgg cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg
    15841 gtaagacttg caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca
    15901 agtgcaaatt ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct
    15961 gtaaacctct attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact
    16021 cacaactttg ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta
    16081 gataacgggg gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct
    16141 tgacacaagg tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt
    16201 tgtcagcaag ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct
    16261 acggatttaa tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct
    16321 tattcgatta caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc
    16381 tttacaaatc atattaagca tgatagactt ataatagcga agatataacg agatatggag
    16441 ggttatgcaa tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg
    16501 ggaatgaaac aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag
    16561 attgaaagag ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg
    16621 gacgttacgc ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag
    16681 gaagtctgtc agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac
    16741 tatgataact tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg
    16801 gaatatatta cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc
    16861 aaagaattta tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac
    16921 agtatggata tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc
    16981 gtgaaaatga taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg
    17041 aataacaatt ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa
    17101 acaaaaagcc cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc
    17161 tttttctttt ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta
    17221 aatcaggcaa aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt
    17281 aattggaaga ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg
    17341 tactctcttt gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt
    17401 taagtatcta tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc
    17461 aatttgatag tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta
    17521 cccagtttaa gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct
    17581 ttgtatgcct atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt
    17641 ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt
    17701 tttagctcat gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg
    17761 agcgattaca gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt
    17821 agaacgtcag agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac
    17881 gaaggtgaac atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc
    17941 attatcagtt ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca
    18001 aactcgtata ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa
    18061 taagattgac caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact
    18121 ttctgccgaa attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa
    18181 ctttaccgaa tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa
    18241 atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt
    18301 tcagaattgt tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga
    18361 taaccttata gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga
    18421 actttgcgga aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat
    18481 acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa
    18541 aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc
    18601 ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg
    18661 agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac
    18721 aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat
    18781 ctcagatagt gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact
    18841 ttctttctta gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca
    18901 tgtggagata gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc
    18961 agaatatacc attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc
    19021 tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata
    19081 cttaaatcaa tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg
    19141 attgtatggt tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag
    19201 ccctgttagt accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt
    19261 aaaaaaagct ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca
    19321 ggaatatctt tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac
    19381 tcaattgaaa aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga
    19441 atatcgtagt gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa
    19501 agggtaccat gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat
    19561 agataaagta cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa
    19621 tatggtttct tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa
    19681 aggaggagat agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa
    19741 cacgattaaa gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat
    19801 gcagacaaga aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag
    19861 acgcaaagac gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc
    19921 cgttatgtat ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg
    19981 ctaaagattc cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct
    20041 gtaatcgtcg ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa
    20101 catgaagtaa cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt
    20161 caatattagt taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta
    20221 aacctcgttt caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat
    20281 cttctttttt acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa
    20341 acaggtatag aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact
    20401 aaacgccaat aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat
    20461 attacttcca aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa
    20521 taaatttcct tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac
    20581 gtccaccaat gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga
    20641 aataaatctg tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc
    20701 agtaatgcaa caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca
    20761 aggctcactt cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag
    20821 catatcgtca gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa
    20881 agtcgaaaca gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc
    20941 gaaacgaaac aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt
    21001 aaagtcttgc actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga
    21061 ctatcagaaa aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga
    21121 gaaatttcag aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga
    21181 ctaaggaagt taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt
    21241 tgaattgatt tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt
    21301 ttatgacggt tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa
    21361 aatcattatg caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc
    21421 aatattgaaa tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca
    21481 cagtttcgtg ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg
    21541 taaattcgtg gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt
    21601 atgcggataa ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca
    21661 tgaagggacc gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc
    21721 ttgttttcct gcacaaacaa gcaatcgctc tacctgttcc atcgcat
//
LOCUS       DAYYBG010000278         9182 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.guided.105, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000278 DAYYBG010000000
VERSION     DAYYBG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.guided.105"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACHAUN_003040"
     CDS             complement(134..577)
                     /locus_tag="ACHAUN_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8461628.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACHAUN_003041"
     CDS             complement(795..2135)
                     /locus_tag="ACHAUN_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8461629.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACHAUN_003042"
     CDS             2404..3300
                     /locus_tag="ACHAUN_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8461630.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACHAUN_003043"
     CDS             complement(3450..4391)
                     /locus_tag="ACHAUN_003043"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8461631.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACHAUN_003044"
     CDS             4517..5638
                     /locus_tag="ACHAUN_003044"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8461632.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHAUN_003045"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHAUN_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8461633.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACHAUN_003046"
     CDS             7465..7992
                     /locus_tag="ACHAUN_003046"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8461634.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..>9182
                     /locus_tag="ACHAUN_003047"
     CDS             8203..>9182
                     /locus_tag="ACHAUN_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8461635.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVA"
BASE COUNT         3060 a         1691 c         1835 g         2596 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aa
//
LOCUS       DAYYBG010000279         7950 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.guided.115, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000279 DAYYBG010000000
VERSION     DAYYBG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.guided.115"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..1452
                     /locus_tag="ACHAUN_003048"
     CDS             16..1452
                     /locus_tag="ACHAUN_003048"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8461636.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1641..4088
                     /locus_tag="ACHAUN_003049"
     CDS             1641..4088
                     /locus_tag="ACHAUN_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8461637.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4318..5385
                     /gene="rlmN"
                     /locus_tag="ACHAUN_003050"
     CDS             4318..5385
                     /gene="rlmN"
                     /locus_tag="ACHAUN_003050"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8461638.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5375..5581
                     /locus_tag="ACHAUN_003051"
     CDS             5375..5581
                     /locus_tag="ACHAUN_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461639.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5578..6681)
                     /locus_tag="ACHAUN_003052"
     CDS             complement(5578..6681)
                     /locus_tag="ACHAUN_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8461640.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7234..7782
                     /gene="aac(6')"
                     /locus_tag="ACHAUN_003053"
     CDS             7234..7782
                     /gene="aac(6')"
                     /locus_tag="ACHAUN_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8461641.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2666 a         1358 c         1730 g         2196 t
ORIGIN      
        1 gaaaggaaga gatgaatgga acacaaagaa cttacctcat ttccaaaaga ttttctgtgg
       61 ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa
      121 tcagtctggg atcgatttgt acggatacct ggaaagacat ttaaaggaac gaacggtgat
      181 ttggcagtag accactatca tcgttataaa gaagatattg ctttaatgaa agaacaaggt
      241 ctgaaagctt accgcttctc tgtagcttgg acacggattt ttcctaatgg acgaggagaa
      301 gtcaatcaag ctgggctcgc tttttatgag agattgatcg acgaattgat tgaaaacgat
      361 attgaaccta tgttgacttt atatcattgg gatcttccac aagcattgca agatgaatat
      421 aatggttggg aatcacgtca gatcattgaa gatttcactc attatgcaga aacattattt
      481 gaggcattcc gcgggaaagt ccattactgg attagtttga atgaacaaaa tatcttcact
      541 tcattaggtt atttgttagc agcacatccg ccaggtgtga cagacccgaa acgaatgtat
      601 gaagttaacc atattgctaa tttagcaaat gcatctgtca tcaataaatt tcacgagtta
      661 gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc caaattatcc aattgacagt
      721 aatcctgaaa atgttctggc tgctgaaaat gcagaagacc tgatggccaa ttattggatg
      781 gatgtctata tgtggggaaa atatccgatt gctgccatga ggttcttaga agaaaaagga
      841 tgggcaccaa cgattgaagc tggcgatgca gaattgctag aatctgctaa accagatttt
      901 cttgggatca attactatca aacggcgacg aatgcattca atccattaaa cggagttggt
      961 gctggaaaaa tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg agttcctgga
     1021 atgtataaaa aagttgagaa tccatttgtc gaacgaacaa attgggactg ggagattgac
     1081 ccagaaggat taaggatcgg actgcgtcgt atcacaagcc gttaccgcat gccggtactg
     1141 attacggaaa atggcttagg agagtatgac aagttgacag aagataaaca aatccatgac
     1201 gactatcgaa tcaattactt acaaagccac gtcaaagcca tcaaagaagc aatttcggat
     1261 ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac
     1321 ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct
     1381 ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga
     1441 gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt
     1501 tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa
     1561 agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt
     1621 gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga
     1681 cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt
     1741 ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga
     1801 ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag
     1861 ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac
     1921 gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat
     1981 ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca
     2041 gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc
     2101 tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat
     2161 tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta
     2221 tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc
     2281 gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc
     2341 gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg
     2401 tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg
     2461 agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag
     2521 agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt
     2581 tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag
     2641 actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg
     2701 agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg
     2761 tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc
     2821 cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt
     2881 tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg
     2941 aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa
     3001 accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa
     3061 cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt
     3121 cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa
     3181 aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc
     3241 tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac
     3301 aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt
     3361 acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat
     3421 tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg
     3481 aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag
     3541 ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca
     3601 gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg
     3661 cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga
     3721 tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta
     3781 cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga
     3841 caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg
     3901 aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag
     3961 cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca
     4021 agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg
     4081 acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca
     4141 tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt
     4201 gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta
     4261 aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg
     4321 gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac
     4381 ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt
     4441 aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt
     4501 atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat
     4561 ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc
     4621 ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc
     4681 gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg
     4741 gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg
     4801 gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat
     4861 gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag tggattagca
     4921 cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac
     4981 gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag
     5041 aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa
     5101 tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta
     5161 ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa
     5221 catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag
     5281 aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc
     5341 ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt
     5401 tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa
     5461 gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa
     5521 gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta
     5581 actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag
     5641 aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt
     5701 tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt
     5761 taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa
     5821 ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta
     5881 tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt
     5941 atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac
     6001 ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata
     6061 agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc
     6121 tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat
     6181 acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg
     6241 tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac
     6301 acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt
     6361 agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa
     6421 caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt
     6481 tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt
     6541 tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc
     6601 atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg
     6661 taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt
     6721 ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga
     6781 taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga
     6841 acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac
     6901 cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga
     6961 aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat
     7021 catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt
     7081 gaataaagtg tttctgtata atttttttat tttataagta agataaataa tgaaagaata
     7141 gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac
     7201 tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat
     7261 aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat
     7321 ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca
     7381 gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca
     7441 ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact
     7501 cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta
     7561 ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca
     7621 tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa
     7681 ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt
     7741 tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca
     7801 gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt
     7861 gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca gaggttgtga
     7921 aaaagtcgct tagtttcgag tagtaagaaa
//
LOCUS       DAYYBG010000280         5765 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.guided.130, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000280 DAYYBG010000000
VERSION     DAYYBG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.guided.130"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            284..748
                     /locus_tag="ACHAUN_003054"
     CDS             284..748
                     /locus_tag="ACHAUN_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8461642.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            732..1361
                     /locus_tag="ACHAUN_003055"
     CDS             732..1361
                     /locus_tag="ACHAUN_003055"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8461643.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1715..3193
                     /gene="msr(C)"
                     /locus_tag="ACHAUN_003056"
     CDS             1715..3193
                     /gene="msr(C)"
                     /locus_tag="ACHAUN_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8461644.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3240..3686
                     /locus_tag="ACHAUN_003057"
     CDS             3240..3686
                     /locus_tag="ACHAUN_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8461645.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3885..4400)
                     /locus_tag="ACHAUN_003058"
     CDS             complement(3885..4400)
                     /locus_tag="ACHAUN_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8461646.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4517..5719
                     /locus_tag="ACHAUN_003059"
     CDS             4517..5719
                     /locus_tag="ACHAUN_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8461647.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1785 a          959 c         1226 g         1795 t
ORIGIN      
        1 gggattcttg caaaaggcga tttcggaaat ttttggcggt gtaaaattat ctactgcaga
       61 agagctatct tctatagttt gataagaatc aataatcttg gttttagcgt tgtgctttct
      121 ttatcatttt ttccgttcat tctacaaaaa atttcttttc tctatacttt tctattgaga
      181 gcgttttttt tattacacag ctgattcttt gacttttttc tttttttatt ctattattga
      241 caagtcaatc gttgacgagt caattattag aaaggaggaa attatgtctt tagaagagat
      301 tagtcacctt ttatatcaaa tcaaattagc cgatcaaaaa atcgtccaga tctttgaaga
      361 aaatatcggt ataagtctta ctagatacga gatcatgatg agactgaaag aagaaaatcc
      421 attgctacaa agcagtttgc aagaaaagtt gcagattgat caaggagcag ttactcgtca
      481 tctgaaggta ttggaagcaa atggctatgt ttcgcgtcag agaaatcctc agaacaaccg
      541 ggaagtgtat gtagcgctaa cagaaaaagc ccagaagaaa atcaagcatt gtgaaatgga
      601 ccataaagat acaggttccg tgctatcccc ggctttcagc gatgtagaaa tgaaacaatt
      661 tctaacactt ttagataaat tgaataccca actttgcaat ataacaacag aaacgaaaga
      721 ggttaataat aatggaacaa aaaaatgatt tttcaacaat tatgactggt agaagatccg
      781 ttcgcgtata tgatgaaaca tacaaaatcc cacacgaaga aatgctggaa atgatcaaag
      841 aagcgacgac tgcaccttct tctgtcaata tgaaaccatg gcgctttgtg gtcgctgaaa
      901 gcgaggaagc aaaagaaatc ttgcgtcctt tgatccgttt caatacgaga caaaatgata
      961 cttcttctgc aatggtcatg atttttggcg atatgcaatg ttacgaatat ggcgaagaaa
     1021 tctacaacca agcttacgaa tcagggaaga tgccaaaaga agtcagagat caacaattag
     1081 ctgcgatcat tccttattac aagagttttt caagagaaga aatgaacgat gtagtcaaag
     1141 tcgattctag cttagcggct atgcaattca tgttagttgc gcgtgatcat ggctatgaaa
     1201 caaatccaat tggtggattc gaagcagatc agttggcaga ggcatttggt ttggataaag
     1261 accgttatgt tccagttatt attttatcaa taggaaaagc ggtagaagaa ggttacgagt
     1321 ctgttcgttt agcaccagaa aagatcacta tgttcgaata aaaggttttc agttactatt
     1381 cagtagctat tgcttttttt gaatcgtgtg tggtaaccta ataaaaaagg aggtcggaaa
     1441 aatgactgca tcgatgaaat tacgtttcga acttttgaat aacaactaaa taaattcaaa
     1501 agacatgtat aaacaacaat ttcatggatg gagtatgtcc attttttcga acgacagtag
     1561 aataggtgaa agctatgaca gatttggtga tttagtcata gttatttcgt tcagagtagg
     1621 aaaagatgtg atgcagtttt tttgcaaaca gatttttctg ctctttttta gtatcgataa
     1681 ttgttgtttt acatgaaata ggagtgtaaa acatatggaa aatttagcag taaatataac
     1741 aaatctacaa gtcagctttg gcaaccagct agaactatct attgattctc ttcgtgtcta
     1801 tcagcaagat cgtataggga tcataggaga aaatggtgtc ggtaaatcga ctttgctcaa
     1861 actaatagcc ggtgaacttt ttcccgatca tgggaaaatc caaacagaga tcaccttcaa
     1921 ctacctgcct caattaacct atctcgctga agcaaaggac ctaaatttgg aattagccag
     1981 tcatttccag ttaagactgg aagaaacttc agagcggaaa tggagcggag gggaagaacg
     2041 aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa gggatgcttc tagacgagcc
     2101 aacaacccat ctagatagaa aaagtattga tcgactgatt gaagagcttc gttattatta
     2161 tggtacgctg gtttttgtta gtcatgatcg ctattttcta gatgagctag catcgaaaat
     2221 ctgggaagta aaagacggag aaatccgaga gttttcgggg aattatagtg cctatctcac
     2281 tcaaaaggaa ttggagaaaa agactcagct acgagaagca gagtcgatca tgaaagagaa
     2341 aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa gcggaaaagt tggaaaaagt
     2401 gtccagtaaa aagaaaaagc aacaaatcag actggatcgg ttgtcttcct ctaaacaaaa
     2461 agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca ttagagagaa gactccaaaa
     2521 aataggagaa acaaccaagc cgcagcagat gaaacaaatc cgttttccag taccaaaatc
     2581 tcttgaactc cacagccgct atcccatcat gggacaaaat gtccaattgg aaagaagtgg
     2641 gagaacatta ctggtaaatg gtgattttca gttttcttta ggtaaaaaaa tcgcgattgt
     2701 cggtgaaaat ggttcaggta agacaacctt attagaacat atccgcaaac aaggagaagg
     2761 aatccttctc tctccgaaag taagctttca agtatatcag caaaagggtt atcaaatgac
     2821 atctgaagaa tccatcattc gttttgtcat gagacaaaca gagttttcag aatcgcttgt
     2881 ccgtagtttg ctgaatcact tagggtttgc tcaggaaact ttgacgaaac cgttatgtac
     2941 attaagtggg ggagaagcga cccgtctgac gattgctttg ctttttacta agccaagtaa
     3001 tgtgttgctg ttagatgaac cgactaattt tattgatatg gcaacgatcg aagctttaga
     3061 gaagctaatg caaatatatc cgggaacgat tttgtttact tctcatgatt cttactttgt
     3121 cgagcgtacg gctgatgaag tttatgaaat aaaagggcag aaaataaaaa aagtacttac
     3181 gagaaatttt taaatgtgct acgatgattt tcgtgacaaa tttaaaggag tggtacagat
     3241 tgcctacaaa taaaaaagaa ggaataattt ttacaacaat gatgtgtttt ttgatggtct
     3301 tggggatgag cgcgtataat ttatggctgc atcaagatct tcatttatca agtttgttga
     3361 ttggtctagt gcctggattc gtggtggctt tcattttgga tgtcttcatc atcggtgtgc
     3421 tggcgaaaaa aattgccttt aaactaccag taaatccaga aagcaaggtc caaatgatct
     3481 taactatttc atgcttgatg gttttaggaa tggtgacatg tatgtcttta ttcggtgtga
     3541 tcatggaaca aggaatacct gatcaattat tctctgctta tatgaatgct tggaaaatga
     3601 atgtgattgc cgctttaccg ttgcagttga tcatcgttgg cccgctttca agaagtgtgc
     3661 taaaagcaat tcaaagtaat gaatgatgga acactgttat ttgaaaaaag gccttggcat
     3721 gctttttgtc atagtctctt gaatgcagtt ctagaaaaat agtgatccga acgataggtt
     3781 ttggcctgtc gttcggatcg ctttttcttt tccaactgta ttgctctttt attgaagata
     3841 atttgctctt caatagatat ttctagaaag gttctgttta tcccttactc aaaaaactgc
     3901 tgcatttgtt ctcttacttg ctgacaccat tggagttcct gctgacaaag cataattctg
     3961 cgctggatca caacataacg tccaaattct tgtttcttgt ctagcgagag gtcattcaat
     4021 tttttttgat ttttttgcat gattttgttc agctgttgtt ctctagtgaa tagcagtcca
     4081 gtagctgttg ttttatcgag cgtcgaaaaa gcatagattt ttgctagaaa ttcatctttt
     4141 tgcgtaggag cgggagtttc ctctttgatc cattcctcta caagagcacg tccttcatca
     4201 gtcaagtggt agatcttttt ttgagagtgc tcgtctttgt ccaaaacggt cacataaccc
     4261 tgctggtcta acttggcaag cgttgtatag atctgactgt gatgggcttc ccaaaaaatt
     4321 gtgatcagct gcttcaattc atacccagaa cgtggtttgg ataaaagcac gcataataat
     4381 atataggcta aagtattcat aaaaatccta cctctcgtct tattttcttt attatatcac
     4441 agtaaagttt ccacgtttta catgtgcata ttgacatgta aaataaatca tgtatgatgc
     4501 taatagagag aaagggatgg aaatattgga gaatcaaata gatatacaag aacaaacaaa
     4561 tgaagaaaca aaggggaaaa gttcagcgct agttgtagca gctacctgta tgatcgcttt
     4621 tatgggagtg ggtctagtag acccaatctt aaaaacgatt gctgcacagt taaacgctac
     4681 gccagctgaa acgaccctgt tatttactag ttacatgtta gtcactggtg tggtgatgct
     4741 atttacaggt ttcttatcta gtcggatcgg tttgaaaaaa acattgatga caggattgtt
     4801 tatcatcgtt gtattcgctg gtcttggcgg attatctggg aatatcggaa tgctgattgg
     4861 gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc tctactgctt tagcaacgat
     4921 cgtcagtatt ttagttggta acacagaaaa agcaatcatg atgtatgaag cagcacttgg
     4981 ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg ggcagtggtt catggcgtta
     5041 tccatttttt ggggtagcat ttttgatgtt ttgcggtttt atagccctag cggtactttt
     5101 acctgaaact gagaaaccaa agagaaaagt aggcctttgg gaaggtgtca aagcgttaag
     5161 caatcataaa cttcgttcag ttggactaat cgcattgtta tataatttcg gattctttac
     5221 tttacttgcg tatgcgccat ttttattgat tggctattct gaattagaag tcggttttgt
     5281 attttttggc tggggtgtgc ttctggctat tgcttcgatt ttcgttgcac cggctcttga
     5341 gcggaagttt acgacttatc ggacaatttt agcagcattt gtcttattca ttatttgttt
     5401 gctgttatta ggagctggaa cttctatgcc agtccttgta ccgatcagca ttgtgttagc
     5461 aggatttttc caaggaatta tcaatacact gttgacgacg attgctatgg aaataccagg
     5521 tctgcaacga aacgttgctt cgtccagcta tagttttgtc cgctttttcg gtggagcttt
     5581 agcaccattt attgcaggtg tgattggtga acatattggt gaaagctatg cgttttattt
     5641 tgcaggtgcc attgttctta tcagtgtatg ttgtatcatt tatcaccgta attattttgc
     5701 tacaaatgaa gaagaataag aaatcagagg ttgtgaaaaa gctgtcctgc atcaagtcat
     5761 aagaa
//
LOCUS       DAYYBG010000281         4804 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.guided.143, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000281 DAYYBG010000000
VERSION     DAYYBG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.guided.143"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..787
                     /gene="vanR-A"
                     /locus_tag="ACHAUN_003060"
     CDS             92..787
                     /gene="vanR-A"
                     /locus_tag="ACHAUN_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM8461648.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="ACHAUN_003061"
     CDS             765..1919
                     /locus_tag="ACHAUN_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM8461649.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="ACHAUN_003062"
     CDS             2134..3102
                     /locus_tag="ACHAUN_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM8461650.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="ACHAUN_003063"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="ACHAUN_003063"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM8461651.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="ACHAUN_003064"
     CDS             4132..4740
                     /locus_tag="ACHAUN_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFM8461652.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          922 c         1119 g         1281 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 ccct
//
LOCUS       DAYYBG010000282         3502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.guided.166, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000282 DAYYBG010000000
VERSION     DAYYBG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.guided.166"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..607
                     /gene="dfrG"
                     /locus_tag="ACHAUN_003065"
     CDS             110..607
                     /gene="dfrG"
                     /locus_tag="ACHAUN_003065"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8461653.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(679..2631)
                     /locus_tag="ACHAUN_003066"
     CDS             complement(679..2631)
                     /locus_tag="ACHAUN_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8461654.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2638..2874)
                     /locus_tag="ACHAUN_003067"
     CDS             complement(2638..2874)
                     /locus_tag="ACHAUN_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461655.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3276..>3502
                     /locus_tag="ACHAUN_003068"
     CDS             3276..>3502
                     /locus_tag="ACHAUN_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8461656.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIV"
BASE COUNT         1069 a          605 c          598 g         1230 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcat aatgagaaaa ggttttttga
       61 tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa tgaaagtttc
      121 tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca ttccttggag
      181 gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga taatattagg
      241 taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata ttattctgac
      301 gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag aagatgtttt
      361 tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga tttataattt
      421 gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat tcgaaggaga
      481 tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc aaaaagggat
      541 aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa aaaacttatt
      601 gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag agcttgggat
      661 tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa attgtttatc
      721 tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt taaaagggcg
      781 acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact tcggattgaa
      841 gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc tttcaagaga
      901 tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat taacatttaa
      961 tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac aatcaactga
     1021 ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg catatttgcg
     1081 atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg ttagtaattc
     1141 agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc tgatatctcc
     1201 ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt ttctgctttt
     1261 cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt ctttcgtgaa
     1321 tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc gccattcgtc
     1381 ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag gagcatcaac
     1441 atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt gaagataagc
     1501 attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc aatctaacct
     1561 atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg tatcttcatt
     1621 taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca catatcttga
     1681 taagacctca gcagatacaa ctatatacgt ttgcatatta aggtcttcaa accccgtata
     1741 tttctttgaa ccttgtcctt tcttacggac gttatttagg tagtagtgca tcttgtccgt
     1801 attaagccac atctcgttaa atttctttgt ttgaaggggt tgtgtctcat atcgttctaa
     1861 aaactccaaa caacgcctat acagccactc cagcttatga tagtaggttc caactccaat
     1921 tcctaaaata tcacaagtac gactgactga aacccttcca acaaccatct tagcgaacat
     1981 tggaagaatg gtgttctttt gttggtgata agtagtggtt tcttctcttt ttggtagaac
     2041 attagtgaac ttcttacacg ccttacattg atagcgttga gatttgcccc tacttttccc
     2101 ccttttgtaa aattgcttcg gctcattgaa tggggttgat tcttcttccg agcagccttc
     2161 tttatgaaat tgataatctg gctcaacatc ttggatgcta ttgatttcta taaggcgttt
     2221 aatttcttca acaacagacc aattagataa aggggttact gtacagtttt gcgatacacc
     2281 tctgccaata ggattgtcat tgcagtacat acctttatga cctttatctt tggaactacc
     2341 agtcatttta tatcgacttg gtttcccttt tacatctttg aatttgtgct gttctttccc
     2401 aaaattacaa caaaacggat tgatgcagta attatattga atgggatatt cagaaccgtt
     2461 ccatttaaaa gacgttggca agaacaacat tttgttatac cttgttgcaa atcgttttgg
     2521 agttaataaa tcatattgtt tagccctatc ttttaattct tcgtttgaaa tcggaatttt
     2581 aacatctaca atttcatctt ccttcgtggc taatctttta agcttggtca ttgtctatca
     2641 acccactcta tctttgatac ccattttttc aacaatccgc ttattacttc gcttgttggc
     2701 aatccattca ataaacttct tatcgtctaa aatgttcaat aagaattcat ccactgcttc
     2761 gctttcagta cgctctgcat attcagcgta ttgcttaact atcaccctta cttgctctga
     2821 tatttcccaa tccacagact tagcgttctt attttttggc tcaagaaatt gcataggaaa
     2881 atgcacccct ttttgcttag taaattgtaa ccattttaac acaccaattt gcattcctac
     2941 aataattatt tcacgttcgc atttttatat acattttaac ttacattttg tggtgtaaat
     3001 taacaacaaa cgttgattta acaataaaaa acgagtatca aattcagttg caatttgata
     3061 ctcatttagg tttacatttt cactttttgt actccaaaac tgaactactt aaatctatac
     3121 tgctgcctct tttttttagt acatgtcctt catcttcgca ggaagtgcca atatgatttt
     3181 aagaaaggta atagacaaaa agtcaaaaag ataaaacaga gtttcttctt tacaaaggga
     3241 atgtatgttc gtataatggt attgaggtga aggttatgga gaaaaaatat ggaacagtag
     3301 ataagatccg tactttgcaa gttagtgcca gaccgttgat ccgtttcagt ttaaatgggg
     3361 tcaattgttt gattgcttcc catagtttga acttcctagc tgaagtagac gaagggatga
     3421 agttagtcgt tacaggttat tataataata gaaagcaatt tgttgtgaga tcctatcatg
     3481 tgttagggaa acccaaaatt gt
//
LOCUS       DAYYBG010000283         3278 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.guided.168, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000283 DAYYBG010000000
VERSION     DAYYBG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.guided.168"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..188
                     /locus_tag="ACHAUN_003069"
     CDS             3..188
                     /locus_tag="ACHAUN_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8461657.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ACHAUN_003070"
     CDS             203..1072
                     /locus_tag="ACHAUN_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8461658.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACHAUN_003071"
     CDS             1053..1787
                     /locus_tag="ACHAUN_003071"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8461659.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACHAUN_003072"
     CDS             1820..2728
                     /locus_tag="ACHAUN_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8461660.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="ACHAUN_003073"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="ACHAUN_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAYYBG010000284         2931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.guided.175, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000284 DAYYBG010000000
VERSION     DAYYBG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.guided.175"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..1079
                     /locus_tag="ACHAUN_003074"
     CDS             168..1079
                     /locus_tag="ACHAUN_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFM8461661.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1232..1717
                     /gene="vanZ-A"
                     /locus_tag="ACHAUN_003075"
     CDS             1232..1717
                     /gene="vanZ-A"
                     /locus_tag="ACHAUN_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFM8461662.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2236..2520
                     /locus_tag="ACHAUN_003076"
     CDS             2236..2520
                     /locus_tag="ACHAUN_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8461663.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
BASE COUNT         1081 a          414 c          544 g          892 t
ORIGIN      
        1 tgaaaattca tctacattgg tgataatagt aaatccagta gggcgaaata attgactgta
       61 atttacgggg caaaacggca caatctcaaa cgagattgtg ccgtttaagg ggaagattct
      121 agaaatattt catacttcca actatatagt taaggaggag actgaaaatg aagaagttgt
      181 tttttttatt gttattgtta ttcttaatat acttaggtta tgactacgtt aatgaagcac
      241 tgttttctca ggaaaaagtc gaatttcaaa attatgatca aaatcccaaa gaacatttag
      301 aaaatagtgg gacttctgaa aatacccaag agaaaacaat tacagaagaa caggtttatc
      361 aaggaaatct gctattaatc aatagtaaat atcctgttcg ccaagaaagt gtgaagtcag
      421 atatcgtgaa tttatctaaa catgacgaat taataaatgg atacgggttg cttgatagta
      481 atatttatat gtcaaaagaa atagcacaaa aattttcaga gatggtcaat gatgctgtaa
      541 agggtggcgt tagtcatttt attattaata gtggctatcg agactttgat gagcaaagtg
      601 tgctttacca agaaatgggg gctgagtatg ccttaccagc aggttatagt gagcataatt
      661 caggtttatc actagatgta ggatcaagct tgacgaaaat ggaacgagcc cctgaaggaa
      721 agtggataga agaaaatgct tggaaatacg ggttcatttt acgttatcca gaggacaaaa
      781 cagagttaac aggaattcaa tatgaaccat ggcatattcg ctatgttggt ttaccacata
      841 gtgcgattat gaaagaaaag aatttcgttc tcgaggaata tatggattac ctaaaagaag
      901 aaaaaaccat ttctgttagt gtaaatgggg aaaaatatga gatcttttat tatcctgtta
      961 ctaaaaatac caccattcat gtgccgacta atcttcgtta tgagatatca ggaaacaata
     1021 tagacggtgt aattgtgaca gtgtttcccg gatcaacaca tactaattca aggaggtaag
     1081 gatggcggaa tgaaaccaac gaaattaatg aacagcatta ttgtactagc acttttgggg
     1141 taacgttagc tttttaattt aaaacccacg ttaactagga cattgctata ctaatgatac
     1201 aacttaaaca aaagaattag aggaaattat attgggaaaa atattatcta gaggattgct
     1261 agctttatat ttagtgacac taatctggtt agtgttattc aaattacaat acaatatttt
     1321 atcagtattt aattatcatc aaagaagtct taacttgact ccatttactg ctactgggaa
     1381 tttcagagag atgatagata atgttataat ctttattcca tttggcttgc ttttgaatgt
     1441 caattttaaa gaaatcggat ttttacctaa gtttgctttt gtactggttt taagtcttac
     1501 ttttgaaata attcaattta tcttcgctat tggagcgaca gacataacag atgtaattac
     1561 aaatactgtt ggaggctttc ttggactgaa attatatggt ttaagcaata agcatatgaa
     1621 tcaaaaaaaa ttagacagag ttattatttt tgtaggtata cttttgctcg tattattgct
     1681 cgtttaccgt acccatttaa gaataaatta cgtgtaagat gtctaaatca agcaatctga
     1741 tctttcatac acataaagat attgaatgaa ttggattaga tggaaaacgg gatgtgggga
     1801 aactcgcccg taggtgtgaa gtgaggggaa aaccggtgat aaagtaaaaa gcttacctaa
     1861 cactatagta acaaagaaag cccaattatc aattttagtg ctgaggaatt ggtctcttta
     1921 ataaatttcc ttaacgttgt aaatccgcat tttcctgacg gtacccccac tagcagccat
     1981 gctttttttg aatctttgta ggacgtttta aagagaatta cccactttaa aattaaacat
     2041 gcgcttagaa tcgctttagg aaacacgatc cagtccaata atcgtcgata aaaacttttg
     2101 aaaaaggttg gtgaaattac ctacttttgg aataatcaca aatcacaagt gattaatcac
     2161 ttgtttatta agatattaaa agctataatt taaataaagc gtgaatttta ttacacaaaa
     2221 agagggggga gaaacttgga actagcattt agagaaagct taaaaaagat gagaggtacc
     2281 aaatcaaaag aaaaattctc ccaagaatta gaaatgagta gatcaaatta ttcacgaata
     2341 gaatcaggaa aatcagatcc aaccataaaa acactagaac aaattgcaaa gttaactaac
     2401 tcaacgctag tagtggattt aatcccaaat gagccaacag aaccagaacc agaaacagaa
     2461 tcagaacaag taacattgga tttagaaatg gaagaagaaa aaagcaatga cttcgtgtga
     2521 ataatgcacg aaatcgttgc ttattttttt taaaagcggt atactagata taacgaaaca
     2581 acgaactgaa tagaaacgaa aaaagagcca tgacacattt ataaaatgtt tgacgacatt
     2641 ttataaatgc atagcccgat aagattgcca aaccaacgct tatcagttag tcagatgaac
     2701 tcttccctcg taagaagtta tttaattaac tttgtttgaa gacggtatat aaccgtacta
     2761 tcattatata gggaaatcag agagttttca agtatctaag ctactgaatt taagaattgt
     2821 taagcaatca atcggaaatc gtttgattgc tttttttgta ttcatttata gaaggtggag
     2881 tttgtatgaa tcatgatgaa tgtaaaactt atataaaaaa tagtttattg g
//
LOCUS       DAYYBG010000285         1056 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.guided.238, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000285 DAYYBG010000000
VERSION     DAYYBG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.guided.238"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..76
                     /locus_tag="ACHAUN_003077"
     CDS             26..76
                     /locus_tag="ACHAUN_003077"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8461664.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="ACHAUN_003078"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="ACHAUN_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8461665.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            943..>1056
                     /locus_tag="ACHAUN_003079"
     CDS             943..>1056
                     /locus_tag="ACHAUN_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HFM8461666.1"
                     /translation="MSRFFKFGKLHVTKGNGDKLLDILLTASKKLKRSLAPT"
BASE COUNT          394 a          182 c          162 g          318 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacg
//
LOCUS       DAYYBG010000286          735 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3460
            SAMEA115706733-rid22593333.guided.255, whole genome shotgun
            sequence.
ACCESSION   DAYYBG010000286 DAYYBG010000000
VERSION     DAYYBG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706733
            Sequence Read Archive: ERR13196467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289974.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 41 of 95
            Pseudo Genes (incomplete)         :: 50 of 95
            Pseudo Genes (internal stop)      :: 23 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706733-rid22593333.guided.255"
                     /isolate="VRE3460"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..401
                     /locus_tag="ACHAUN_003080"
                     /pseudo
     CDS             52..401
                     /locus_tag="ACHAUN_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          223 a          140 c          116 g          256 t
ORIGIN      
        1 gctttaagca ttacttaaag ataacttaaa agttatcttt taaatactta attgttaaat
       61 accttatttg atgcaaaaga aatctacgat agtcaacaaa aaaattttca ttatatccag
      121 aaaaattttt cttagttaag gacctatgga ttgcagtaat gcagaattcg tcgaataaac
      181 tgccaaagac atacaatcat attgctttta aaattgacga acaagatata gattcgtttg
      241 tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg
      301 aggcttcttc aatttacttt tacgactatg ataatcacct cttcgagtta cataccggta
      361 ctctccagga acgtttaaaa tcctacaatt ccactacctg agactagtaa tgactagtct
      421 ctttttatgg cctctgatcc ccccattgag cgaatactaa tagggcttcg aattgatctg
      481 ttcaatcttt aagcaatatc cctcaaatga gtttggaaag tttagtgatt catcacaaat
      541 acttaaatac atgtagttat atatagattg aggttcttgt gagcgtatgc tcgtgtgggt
      601 tcaaatccca tcgtctgcat ccatctttcc aaaacaagcg gctaggacat ttttgtctca
      661 gccgcttgtt ttctagttta ttctttcatc ttgtcggacc ttttttcttg ggtatagact
      721 tttctcatat tctac
//
